Rh6DG232000

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000448
Physical Location & Seq
Reverse (-)
41371513 .. 41377000
5488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG232000.1

Sequence Viewer

Length: 1143 bp
ATGGAAAACAAATGGGTTCTTCCTCTAGCCCTATCCTTGTTTATAACTGTTCTTAGCTTGGCAGCAGCAGCTAAGCCAGTACTGCCACCAGTCTTCATATTTGGTGATTCAACAGCAGATGTCGGCACCAATAACTTCTTGCCGGCTAGCAAGGCAAGAGCTGACTTCCCCCCCAATGGCATTGACTTTCCTGGCACCTCAAAACCCACCGGAAGGTTCAGCAATGGCCTTAACAGTGCTGATTTTCTTGCCCGGTATTTTGGTTACAAGAGAAGTCCAAGCCCCTTTCTTTCTCTGAAAACTACTTCTCTTCAAAAGAAGAAGTTTAATGGTATCAACTTTGCTTCTGGAGGGTCTGGTCTTCTTGACACAACTGGACAGACAATGCTGACATTGATGAAGTTTGGAACTGGAAATATTCCATTTGGTGCCTCATTCAAAAACCAGAAAAAATTTATCTCATTAACAGAGCAGATACAGCAATTTTCATCTGTCAAGAACAATCTTACGGCCTTGATGGGTTCGGTAGCAACTGAGAAGTTTCTTAAAGATTCTTTGATCTTCATCAGCACCGGCAGCAATGACCTTTTCGGATACTACCATTCAAAGAGTTCCATTCCGAAGGAAAAGTTCTTGTCCTCTTTAGAAGTAGCTTATGAGAATCACTTGAAGACTCTATACAATCTTGGAGCAAGGAAGTTTGGCATCATCAGCATTGCCCCGATTGGCTGCTGCCCATCTCAGAGGATTTTCAATGCTACTGGGGGATGTTTGGAAGAGCTGAATGATAATGCAATAGCTTTTCATTCAAGATTGGATGCCCTCTTGTGCAAGCTTAGCTCAGAATACAAGGGCATGAAGTACTCACTTGGAAATTCATATGAAATGACAATCAATGTCATACAGAACCCTCTTCCATTCAATTTTACACAAGTGGCAGCTGCATGTTGTGGAACTGGGAAGCTCAATGCTGAAAACTTCTGTAAACCAGATGCAAATCTCTGTTCGAATCGCGATCAATACTTGTTCTGGGATCGATTTCATCCAACACAGGCTGCTTATAAGTTGGCTGCTGTAACCCTCTATGGTGGTGGACCACAATTTGTAACCCCAATTAATTTTGCTCAGTTGGCTGAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

380

Amino Acids

41.38

Weight (kDa)

9.18

Isoelectric Point (pI)

21.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 358 8.7e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 44, 1062
AccB1I GGYRCC 3 cut(s) 125, 194, 428
AccII CGCG 1 cut(s) 1014
AclWI GGATC 1 cut(s) 1041
AcsI RAATTY 2 cut(s) 452, 874
AfaI GTAC 2 cut(s) 81, 863
AfiI CCNNNNNNNGG 2 cut(s) 176, 213
AgsI TTSAA 8 cut(s) 111, 314, 439, 606, 670, 754, 810, 922
AjnI CCWGG 1 cut(s) 190
AlwI GGATC 1 cut(s) 1041
AoxI GGCC 2 cut(s) 226, 510
ApoI RAATTY 2 cut(s) 452, 874
AseI ATTAAT 1 cut(s) 1116
AspS9I GGNCC 1 cut(s) 1094
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 116
AsuII TTCGAA 1 cut(s) 1007
AsuNHI GCTAGC 1 cut(s) 146
AvaII GGWCC 1 cut(s) 1094
BanI GGYRCC 3 cut(s) 125, 194, 428
BarI GAAGNNNNNNTAC 2 cut(s) 662, 694
BbsI GAAGAC 3 cut(s) 85, 353, 677
BbvCI CCTCAGC 1 cut(s) 1134
BccI CCATC 2 cut(s) 511, 745
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 1 cut(s) 192
BciVI GTATCC 1 cut(s) 587
BcnI CCSGG 1 cut(s) 253
BfaI CTAG 2 cut(s) 26, 147
BfuI GTATCC 1 cut(s) 587
BlpI GCTNAGC 2 cut(s) 72, 836
BmcAI AGTACT 2 cut(s) 81, 863
Bme1390I CCNGG 2 cut(s) 192, 253
Bme18I GGWCC 1 cut(s) 1094
BmgT120I GGNCC 1 cut(s) 1094
BmiI GGNNCC 3 cut(s) 127, 196, 430
BmrFI CCNGG 2 cut(s) 192, 253
BmrI ACTGGG 2 cut(s) 771, 966
BmsI GCATC 3 cut(s) 714, 808, 982
BmtI GCTAGC 1 cut(s) 150
BmuI ACTGGG 2 cut(s) 771, 966
BpiI GAAGAC 3 cut(s) 85, 353, 677
BpmI CTGGAG 1 cut(s) 369
Bpu10I CCTNAGC 1 cut(s) 1134
Bpu1102I GCTNAGC 2 cut(s) 72, 836
Bpu14I TTCGAA 1 cut(s) 1007
BpuMI CCSGG 1 cut(s) 253
Bsa29I ATCGAT 1 cut(s) 1036
BsaBI GATNNNNATC 2 cut(s) 563, 996
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 213
Bse118I RCCGGY 2 cut(s) 142, 572
Bse1I ACTGG 6 cut(s) 77, 89, 379, 415, 766, 961
Bse3DI GCAATG 3 cut(s) 229, 586, 714
Bse8I GATNNNNATC 2 cut(s) 563, 996
BseBI CCWGG 1 cut(s) 192
BseCI ATCGAT 1 cut(s) 1036
BseGI GGATG 3 cut(s) 773, 823, 1042
BseJI GATNNNNATC 2 cut(s) 563, 996
BseLI CCNNNNNNNGG 2 cut(s) 176, 213
BseMI GCAATG 3 cut(s) 229, 586, 714
BseMII CTCAG 5 cut(s) 525, 755, 855, 1125, 1139
BseNI ACTGG 6 cut(s) 77, 89, 379, 415, 766, 961
Bsh1236I CGCG 1 cut(s) 1014
BshFI GGCC 2 cut(s) 228, 512
BshNI GGYRCC 3 cut(s) 125, 194, 428
BshVI ATCGAT 1 cut(s) 1036
BsiSI CCGG 4 cut(s) 143, 210, 253, 573
BslI CCNNNNNNNGG 2 cut(s) 176, 213
BsnI GGCC 2 cut(s) 228, 512
Bsp119I TTCGAA 1 cut(s) 1007
Bsp143I GATC 3 cut(s) 558, 1015, 1033
Bsp1720I GCTNAGC 2 cut(s) 72, 836
Bsp68I TCGCGA 1 cut(s) 1014
BspANI GGCC 2 cut(s) 228, 512
BspCNI CTCAG 5 cut(s) 526, 754, 854, 1126, 1138
BspDI ATCGAT 1 cut(s) 1036
BspFNI CGCG 1 cut(s) 1014
BspLI GGNNCC 3 cut(s) 127, 196, 430
BspOI GCTAGC 1 cut(s) 150
BspPI GGATC 1 cut(s) 1041
BspQI GCTCTTC 1 cut(s) 771
BspT104I TTCGAA 1 cut(s) 1007
BspT107I GGYRCC 3 cut(s) 125, 194, 428
BsrDI GCAATG 3 cut(s) 229, 586, 714
BsrFI RCCGGY 2 cut(s) 142, 572
BsrI ACTGG 6 cut(s) 77, 89, 379, 415, 766, 961
BssAI RCCGGY 2 cut(s) 142, 572
BssMI GATC 3 cut(s) 558, 1015, 1033
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 49, 236
Bst6I CTCTTC 3 cut(s) 315, 771, 918
BstBI TTCGAA 1 cut(s) 1007
BstC8I GCNNGC 3 cut(s) 144, 148, 833
BstDEI CTNAG 8 cut(s) 53, 72, 534, 741, 836, 841, 1125, 1134
BstF5I GGATG 3 cut(s) 773, 823, 1042
BstFNI CGCG 1 cut(s) 1014
BstKTI GATC 3 cut(s) 561, 1018, 1036
BstMBI GATC 3 cut(s) 558, 1015, 1033
BstMWI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 478, 576, 711, 837, 1130
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 948
BstSCI CCNGG 2 cut(s) 190, 251
BstUI CGCG 1 cut(s) 1014
BstV2I GAAGAC 3 cut(s) 85, 353, 677
Bsu15I ATCGAT 1 cut(s) 1036
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 2 cut(s) 228, 512
BsuTUI ATCGAT 1 cut(s) 1036
BtsCI GGATG 3 cut(s) 773, 823, 1042
BtsIMutI CAGTG 1 cut(s) 241
BtuMI TCGCGA 1 cut(s) 1014
Cac8I GCNNGC 3 cut(s) 144, 148, 833
Cfr10I RCCGGY 2 cut(s) 142, 572
Cfr13I GGNCC 1 cut(s) 1094
ClaI ATCGAT 1 cut(s) 1036
Csp6I GTAC 2 cut(s) 80, 862
CviAII CATG 2 cut(s) 856, 945
CviQI GTAC 2 cut(s) 80, 862
DdeI CTNAG 8 cut(s) 53, 72, 534, 741, 836, 841, 1125, 1134
DpnI GATC 3 cut(s) 560, 1017, 1035
DpnII GATC 3 cut(s) 558, 1015, 1033
Eam1104I CTCTTC 3 cut(s) 315, 771, 918
EarI CTCTTC 3 cut(s) 315, 771, 918
Eco47I GGWCC 1 cut(s) 1094
EcoRII CCWGG 1 cut(s) 190
FaeI CATG 2 cut(s) 859, 948
FatI CATG 2 cut(s) 855, 944
FauNDI CATATG 1 cut(s) 880
FokI GGATG 3 cut(s) 780, 830, 1029
FspBI CTAG 2 cut(s) 26, 147
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 2 cut(s) 228, 512
HapII CCGG 4 cut(s) 143, 210, 253, 573
Hin1II CATG 2 cut(s) 859, 948
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 5 cut(s) 107, 551, 661, 673, 1009
HpaII CCGG 4 cut(s) 143, 210, 253, 573
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 2 cut(s) 986, 1094
Hpy188I TCNGA 5 cut(s) 297, 593, 621, 744, 844
Hpy188III TCNNGA 5 cut(s) 348, 365, 496, 810, 1013
Hpy8I GTNNAC 2 cut(s) 986, 1094
HpyAV CCTTC 2 cut(s) 207, 616
HpyCH4III ACNGT 2 cut(s) 49, 236
HpyCH4V TGCA 4 cut(s) 794, 831, 944, 995
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 478, 576, 711, 837, 1130
HpyF3I CTNAG 8 cut(s) 53, 72, 534, 741, 836, 841, 1125, 1134
Hsp92II CATG 2 cut(s) 859, 948
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Kzo9I GATC 3 cut(s) 558, 1015, 1033
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 1 cut(s) 689
LweI GCATC 3 cut(s) 714, 808, 982
MaeI CTAG 2 cut(s) 26, 147
MaeIII GTNAC 3 cut(s) 263, 1075, 1105
MalI GATC 3 cut(s) 560, 1017, 1035
MboI GATC 3 cut(s) 558, 1015, 1033
MboII GAAGA 9 cut(s) 11, 85, 302, 331, 353, 553, 682, 788, 905
MluCI AATT 7 cut(s) 452, 482, 874, 922, 1100, 1113, 1117
MlyI GAGTC 1 cut(s) 667
MmeI TCCRAC 1 cut(s) 1070
MroNI GCCGGC 1 cut(s) 142
MseI TTAA 6 cut(s) 231, 327, 464, 546, 1116, 1141
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 4 cut(s) 143, 210, 253, 573
MspR9I CCNGG 2 cut(s) 192, 253
MvaI CCWGG 1 cut(s) 192
MvnI CGCG 1 cut(s) 1014
MwoI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 478, 576, 711, 837, 1130
NaeI GCCGGC 1 cut(s) 144
NciI CCSGG 1 cut(s) 253
NdeI CATATG 1 cut(s) 880
NdeII GATC 3 cut(s) 558, 1015, 1033
NgoMIV GCCGGC 1 cut(s) 142
NheI GCTAGC 1 cut(s) 146
NlaIII CATG 2 cut(s) 859, 948
NlaIV GGNNCC 3 cut(s) 127, 196, 430
NruI TCGCGA 1 cut(s) 1014
NspI RCATGY 1 cut(s) 948
NspV TTCGAA 1 cut(s) 1007
PciSI GCTCTTC 1 cut(s) 771
PdiI GCCGGC 1 cut(s) 144
PfeI GAWTC 4 cut(s) 107, 551, 661, 1009
PleI GAGTC 1 cut(s) 667
PpsI GAGTC 1 cut(s) 667
PshBI ATTAAT 1 cut(s) 1116
PsiI TTATAA 2 cut(s) 44, 1062
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 3 cut(s) 127, 196, 430
PspPI GGNCC 1 cut(s) 1094
PvuII CAGCTG 1 cut(s) 941
RruI TCGCGA 1 cut(s) 1014
RsaI GTAC 2 cut(s) 81, 863
RsaNI GTAC 2 cut(s) 80, 862
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 6 cut(s) 231, 327, 464, 546, 1116, 1141
Sau3AI GATC 3 cut(s) 558, 1015, 1033
Sau96I GGNCC 1 cut(s) 1094
ScaI AGTACT 2 cut(s) 81, 863
SchI GAGTC 1 cut(s) 667
ScrFI CCNGG 2 cut(s) 192, 253
SfaNI GCATC 3 cut(s) 714, 808, 982
SfuI TTCGAA 1 cut(s) 1007
SinI GGWCC 1 cut(s) 1094
Sse9I AATT 7 cut(s) 452, 482, 874, 922, 1100, 1113, 1117
SspI AATATT 1 cut(s) 418
SspMI CTAG 2 cut(s) 26, 147
StyD4I CCNGG 2 cut(s) 190, 251
TaaI ACNGT 2 cut(s) 49, 236
TaqI TCGA 2 cut(s) 1007, 1036
TasI AATT 7 cut(s) 452, 482, 874, 922, 1100, 1113, 1117
TatI WGTACW 2 cut(s) 79, 861
TfiI GAWTC 4 cut(s) 107, 551, 661, 1009
Tru1I TTAA 6 cut(s) 231, 327, 464, 546, 1116, 1141
Tru9I TTAA 6 cut(s) 231, 327, 464, 546, 1116, 1141
TscAI CASTG 1 cut(s) 241
TspDTI ATGAA 9 cut(s) 85, 413, 477, 553, 794, 867, 872, 897, 1031
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 1094
VspI ATTAAT 1 cut(s) 1116
XapI RAATTY 2 cut(s) 452, 874
XceI RCATGY 1 cut(s) 948
XspI CTAG 2 cut(s) 26, 147
ZrmI AGTACT 2 cut(s) 81, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.