Rh2AG116800

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000448
Physical Location & Seq
Forward (+)
10524087 .. 10525358
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG116800.1

Sequence Viewer

Length: 741 bp
ATGGCCAATAACAATGGGTGGACACCCTTGTTTTCTGTTTGGCTTTTTGTGTTTGTATATCTGGCCATGCTAGGTTTCCATGCATCCTCTTCACAGCAAATTGTGCCTGCAATTTACATATTTGGAGATTCTTCAGGAGATGTTGGAACCAATAATTACTTGCCTGATTGTACCGCAAGGGCTGATATCCTCTATAATGGGATTGACGACTCTCACTCTAAACCAACAGGAAGATTTAGCAACGGTTACAACGTCGTAGATTACATCGCCCAGTTTCTGGGTTACAAGAAGAGCCCGCCACCTTTTCTCTCTCTTTTAGACGAGAAGAAGCAATTTCCTAACAGGAAGGTTCCAAATAAGGGAGTTAACTTTGCGTCAGGAGGATCAGGACTTCTGGACGATACTGGTAAACTACAGTGGGGAAATGTCATTTCCTTGGGGGAACAAGTGCAGCAATTCCAAACTTTACGGAACAAAATCTCAGAATTATTGGGTGCTGGTGGATTGGCTAATATTTCTCAGTCTCTCTTTGTCATCAGCGTTGGAAGCAACGACATCTTTGAATTATTTGCTGCTAATAACCTGACTAAAAGAACCGAGCAAGACTACTTGAGCATACTCATCTCCTCTTACGAAACTCATTCAAGGAACTTATTTATGCTCGGAGGCCGGAGCAAAGAAGTTTGGGATTATCAGCGTCTCACAAATTGGGTGCCGCCCTGCCCAGCGAAAGCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

246

Amino Acids

27.24

Weight (kDa)

6.82

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 218 4.5e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 712
AccB7I CCANNNNNTGG 1 cut(s) 277
AciI CCGC 3 cut(s) 174, 296, 716
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 2 cut(s) 3, 63
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 1 cut(s) 172
AfiI CCNNNNNNNGG 2 cut(s) 277, 359
AgsI TTSAA 2 cut(s) 563, 645
AluBI AGCT 1 cut(s) 734
AluI AGCT 1 cut(s) 734
Alw26I GTCTC 2 cut(s) 528, 704
AlwI GGATC 1 cut(s) 391
AlwNI CAGNNNCTG 1 cut(s) 277
AoxI GGCC 3 cut(s) 3, 63, 667
ApeKI GCWGC 2 cut(s) 451, 572
BalI TGGCCA 2 cut(s) 5, 65
BanI GGYRCC 1 cut(s) 712
BanII GRGCYC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 463, 559
BcoDI GTCTC 2 cut(s) 528, 704
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 413
BisI GCNGC 3 cut(s) 452, 573, 716
BlsI GCNGC 3 cut(s) 453, 574, 717
BmiI GGNNCC 3 cut(s) 148, 351, 714
BmrI ACTGGG 1 cut(s) 265
BmsI GCATC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 265
BpuEI CTTGAG 1 cut(s) 631
BsaJI CCNNGG 1 cut(s) 435
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 359
Bse1I ACTGG 2 cut(s) 271, 409
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 1 cut(s) 83
BseLI CCNNNNNNNGG 2 cut(s) 277, 359
BseMII CTCAG 2 cut(s) 495, 533
BseNI ACTGG 2 cut(s) 271, 409
BseRI GAGGAG 1 cut(s) 616
BseXI GCAGC 2 cut(s) 463, 559
BseYI CCCAGC 1 cut(s) 724
BsgI GTGCAG 1 cut(s) 470
BshFI GGCC 3 cut(s) 5, 65, 669
BshNI GGYRCC 1 cut(s) 712
BsiSI CCGG 1 cut(s) 670
BslI CCNNNNNNNGG 2 cut(s) 277, 359
BsmAI GTCTC 2 cut(s) 528, 704
BsmBI CGTCTC 1 cut(s) 704
BsnI GGCC 3 cut(s) 5, 65, 669
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 1 cut(s) 383
BspACI CCGC 3 cut(s) 174, 296, 716
BspANI GGCC 3 cut(s) 5, 65, 669
BspCNI CTCAG 2 cut(s) 494, 532
BspLI GGNNCC 3 cut(s) 148, 351, 714
BspPI GGATC 1 cut(s) 391
BspQI GCTCTTC 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 712
BsrI ACTGG 2 cut(s) 271, 409
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 1 cut(s) 383
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 2 cut(s) 245, 417
Bst6I CTCTTC 2 cut(s) 94, 284
BstAPI GCANNNNNTGC 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 108, 296
BstDEI CTNAG 3 cut(s) 481, 519, 735
BstF5I GGATG 1 cut(s) 83
BstKTI GATC 1 cut(s) 386
BstMAI GTCTC 2 cut(s) 528, 704
BstMBI GATC 1 cut(s) 383
BstMWI GCNNNNNNNGC 2 cut(s) 103, 546
BstSFI CTRYAG 1 cut(s) 413
BstV1I GCAGC 2 cut(s) 463, 559
BsuRI GGCC 3 cut(s) 5, 65, 669
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 108, 296
CaiI CAGNNNCTG 1 cut(s) 277
CseI GACGC 2 cut(s) 363, 686
Csp6I GTAC 1 cut(s) 171
CviAII CATG 2 cut(s) 67, 80
CviJI RGCY 8 cut(s) 5, 43, 65, 182, 294, 509, 669, 734
CviKI_1 RGCY 8 cut(s) 5, 43, 65, 182, 294, 509, 669, 734
CviQI GTAC 1 cut(s) 171
DdeI CTNAG 3 cut(s) 481, 519, 735
DpnI GATC 1 cut(s) 385
DpnII GATC 1 cut(s) 383
EaeI YGGCCR 2 cut(s) 3, 63
Eam1104I CTCTTC 2 cut(s) 94, 284
EarI CTCTTC 2 cut(s) 94, 284
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 117
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 435
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 704
FaeI CATG 2 cut(s) 70, 83
FaiI YATR 7 cut(s) 58, 68, 81, 119, 195, 617, 659
FatI CATG 2 cut(s) 66, 79
FauI CCCGC 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 452, 573, 716
FokI GGATG 1 cut(s) 70
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 3 cut(s) 452, 573, 716
FspBI CTAG 1 cut(s) 71
GluI GCNGC 3 cut(s) 452, 573, 716
GsaI CCCAGC 1 cut(s) 728
HaeIII GGCC 3 cut(s) 5, 65, 669
HapII CCGG 1 cut(s) 670
HgaI GACGC 2 cut(s) 363, 686
Hin1II CATG 2 cut(s) 70, 83
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 2 cut(s) 128, 209
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 1 cut(s) 670
Hpy166II GTNNAC 3 cut(s) 21, 367, 410
Hpy188I TCNGA 2 cut(s) 484, 665
Hpy188III TCNNGA 4 cut(s) 135, 378, 387, 395
Hpy8I GTNNAC 3 cut(s) 21, 367, 410
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 2 cut(s) 245, 417
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 3 cut(s) 83, 110, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 546
HpyF3I CTNAG 3 cut(s) 481, 519, 735
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 2 cut(s) 70, 83
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 1 cut(s) 383
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 1 cut(s) 672
Lsp1109I GCAGC 2 cut(s) 463, 559
LweI GCATC 1 cut(s) 92
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 245, 281
MalI GATC 1 cut(s) 385
MboI GATC 1 cut(s) 383
MboII GAAGA 5 cut(s) 81, 123, 243, 301, 337
MhlI GDGCHC 1 cut(s) 296
MlsI TGGCCA 2 cut(s) 5, 65
MluCI AATT 8 cut(s) 99, 111, 154, 332, 455, 485, 563, 706
MluNI TGGCCA 2 cut(s) 5, 65
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 2 cut(s) 124, 523
MnlI CCTC 5 cut(s) 97, 200, 374, 637, 659
Mox20I TGGCCA 2 cut(s) 5, 65
Mph1103I ATGCAT 1 cut(s) 85
MscI TGGCCA 2 cut(s) 5, 65
MseI TTAA 1 cut(s) 366
Msp20I TGGCCA 2 cut(s) 5, 65
MspI CCGG 1 cut(s) 670
MwoI GCNNNNNNNGC 2 cut(s) 103, 546
NdeII GATC 1 cut(s) 383
NlaIII CATG 2 cut(s) 70, 83
NlaIV GGNNCC 3 cut(s) 148, 351, 714
NsiI ATGCAT 1 cut(s) 85
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 1 cut(s) 128
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 3 cut(s) 453, 574, 717
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PspFI CCCAGC 1 cut(s) 724
PspN4I GGNNCC 3 cut(s) 148, 351, 714
PstNI CAGNNNCTG 1 cut(s) 277
RsaI GTAC 1 cut(s) 172
RsaNI GTAC 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 3 cut(s) 452, 573, 716
Sau3AI GATC 1 cut(s) 383
SchI GAGTC 1 cut(s) 203
SduI GDGCHC 1 cut(s) 296
SetI ASST 6 cut(s) 76, 255, 304, 351, 585, 736
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
Sse9I AATT 8 cut(s) 99, 111, 154, 332, 455, 485, 563, 706
SsiI CCGC 3 cut(s) 174, 296, 716
SspI AATATT 1 cut(s) 514
SspMI CTAG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 245, 417
TaiI ACGT 1 cut(s) 255
TasI AATT 8 cut(s) 99, 111, 154, 332, 455, 485, 563, 706
TauI GCSGC 1 cut(s) 718
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 1 cut(s) 422
TseI GCWGC 2 cut(s) 451, 572
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 1 cut(s) 422
Van91I CCANNNNNTGG 1 cut(s) 277
XspI CTAG 1 cut(s) 71
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.