Rroxscaffold_2G00144410

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000448
Physical Location & Seq
Reverse (-)
82316032 .. 82323709
7678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144410.1

Sequence Viewer

Length: 1119 bp
ATGGCCAATAACAATGGGTGGACACCCTTGTTTTCTGTTTGGCTCTTTGTGTTTGTATATCTGGCCATGCTAGGTTTCCATGAATCCTCTTCACAGCAAATTGTGCCTGCAATTTACATATTTGGAGATTCTACAGGAGATGTTGGAACCAATAATTACTTGCCTGATTGTACTGCAAGGGCTGATATCCTCTATAATGGGATTGACTACCCTCACTCTAAACCAACAGGAAGATTTAGCAACGGTTACAACGTCGTAGATTACATCGTTAGCCCAGTTTCTTACAAGAAGAGCCCGCCACCTTTTCTCTCTCTTTCAGACGAGAAGAAGCAATTTCCTAACAGGAAGGTTCCAAATAAGGGAGTTAACTTTGCGTCGGGAGGATCGGGACTTCTAAAATCTTATGTGTACGTACAACAGGGAAATGTCATTTCCTTGGGGGAACAAGTGCAGCAATTCCAAACTTTACGGAAAAAAATCTCAGAATTATTGGGTGCTGGTGGATTGGCTAATATTTCTCAATCTCTCTTTGTCATCAGCGTTGGAAGTAACGACATCTTTGAATTATTCGCTGCTAATAACCCGACTAAAAGAACCGAGCAAGACTACTTGAGCATACTCATCTCCTCTTATGAAACTCATTTAAGGAATTTATATGAGCTAGGAGCCAGAAGATTTGGGATTATTAGTGTTGGGGCACTCGGGTGCTGTCCGATCCTACGTAGTAAATATGCCGGTTGTTGTTTGGAGCCCATGAATAGCGATGCTCGGTTGTTCTATAGTGCACTCCGAGACCTCTTGCAAAAGTTGAGTTCAGAGTGCAAAGGGTTGATGTATGCACTTGGAGATTCATATAATATGTCAAAATATATTCTCGACCACCCCGATAAATCTGTTTTTTCCGAGGTTAAAAGAGCTTGCTGTGGAAAAGGAAAGTTGAACGCACATGAGGCATGTAAACCGAATTCGGATTTGTGTAAAGATCGCACAACCTACTTGTTTTGGGACCAGTACCATCCAACACAGGAGGCTTCTTCTCTCGCAGCCAGTATTCTTTATACTGGTAACAAACCATTCGTGGTGCCGATGGGTTTTGGTGAATTGGCCCTTCTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

372

Amino Acids

41.13

Weight (kDa)

8.4

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 350 1.2e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1081
AciI CCGC 1 cut(s) 296
AclWI GGATC 2 cut(s) 391, 709
AcoI YGGCCR 2 cut(s) 3, 63
AcsI RAATTY 2 cut(s) 649, 964
AfaI GTAC 4 cut(s) 172, 410, 414, 1013
AfiI CCNNNNNNNGG 1 cut(s) 359
AgsI TTSAA 2 cut(s) 563, 940
AleI CACNNNNGTG 1 cut(s) 703
AluBI AGCT 2 cut(s) 661, 917
AluI AGCT 2 cut(s) 661, 917
Alw21I GWGCWC 1 cut(s) 787
Alw26I GTCTC 1 cut(s) 786
Alw44I GTGCAC 1 cut(s) 783
AlwI GGATC 2 cut(s) 391, 709
Ama87I CYCGRG 1 cut(s) 701
AoxI GGCC 3 cut(s) 3, 63, 1104
ApaLI GTGCAC 1 cut(s) 783
ApeKI GCWGC 3 cut(s) 451, 572, 1043
ApoI RAATTY 2 cut(s) 649, 964
AspS9I GGNCC 2 cut(s) 1006, 1105
AsuHPI GGTGA 1 cut(s) 1109
AvaI CYCGRG 1 cut(s) 701
AvaII GGWCC 1 cut(s) 1006
BaeGI GKGCMC 2 cut(s) 700, 787
BalI TGGCCA 2 cut(s) 5, 65
BanI GGYRCC 1 cut(s) 1081
BanII GRGCYC 2 cut(s) 296, 753
Bbv12I GWGCWC 1 cut(s) 787
BbvI GCAGC 3 cut(s) 463, 559, 1055
BccI CCATC 2 cut(s) 1023, 1081
BcoDI GTCTC 1 cut(s) 786
BfaI CTAG 2 cut(s) 71, 662
BfmI CTRYAG 2 cut(s) 132, 778
BisI GCNGC 3 cut(s) 452, 573, 1044
BlsI GCNGC 3 cut(s) 453, 574, 1045
Bme18I GGWCC 1 cut(s) 1006
BmeT110I CYCGRG 1 cut(s) 701
BmgT120I GGNCC 2 cut(s) 1006, 1105
BmiI GGNNCC 6 cut(s) 148, 351, 667, 750, 1007, 1083
BmrI ACTGGG 1 cut(s) 269
BmsI GCATC 1 cut(s) 754
BmuI ACTGGG 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 631
BsaAI YACGTR 2 cut(s) 412, 722
BsaI GGTCTC 1 cut(s) 786
BsaJI CCNNGG 2 cut(s) 435, 903
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse118I RCCGGY 1 cut(s) 734
Bse1I ACTGG 4 cut(s) 275, 1009, 1047, 1066
BseDI CCNNGG 2 cut(s) 435, 903
BseGI GGATG 1 cut(s) 1015
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 4 cut(s) 275, 1009, 1047, 1066
BseRI GAGGAG 1 cut(s) 616
BseSI GKGCMC 2 cut(s) 700, 787
BseXI GCAGC 3 cut(s) 463, 559, 1055
BsgI GTGCAG 1 cut(s) 470
BshFI GGCC 3 cut(s) 5, 65, 1106
BshNI GGYRCC 1 cut(s) 1081
BsiHKAI GWGCWC 1 cut(s) 787
BsiHKCI CYCGRG 1 cut(s) 701
BsiSI CCGG 1 cut(s) 735
BslFI GGGAC 2 cut(s) 402, 1019
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 1 cut(s) 786
BsmFI GGGAC 2 cut(s) 402, 1019
BsnI GGCC 3 cut(s) 5, 65, 1106
Bso31I GGTCTC 1 cut(s) 786
BsoBI CYCGRG 1 cut(s) 701
Bsp1286I GDGCHC 4 cut(s) 296, 700, 753, 787
Bsp143I GATC 3 cut(s) 383, 714, 982
BspACI CCGC 1 cut(s) 296
BspANI GGCC 3 cut(s) 5, 65, 1106
BspCNI CTCAG 1 cut(s) 494
BspLI GGNNCC 6 cut(s) 148, 351, 667, 750, 1007, 1083
BspPI GGATC 2 cut(s) 391, 709
BspQI GCTCTTC 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 1081
BspTNI GGTCTC 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 734
BsrI ACTGG 4 cut(s) 275, 1009, 1047, 1066
BssAI RCCGGY 1 cut(s) 734
BssECI CCNNGG 2 cut(s) 435, 903
BssMI GATC 3 cut(s) 383, 714, 982
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 245
Bst6I CTCTTC 2 cut(s) 94, 284
BstAPI GCANNNNNTGC 1 cut(s) 103
BstBAI YACGTR 2 cut(s) 412, 722
BstC8I GCNNGC 3 cut(s) 108, 296, 919
BstDEI CTNAG 1 cut(s) 481
BstF5I GGATG 1 cut(s) 1015
BstKTI GATC 3 cut(s) 386, 717, 985
BstMAI GTCTC 1 cut(s) 786
BstMBI GATC 3 cut(s) 383, 714, 982
BstMWI GCNNNNNNNGC 2 cut(s) 103, 950
BstNSI RCATGY 1 cut(s) 957
BstSFI CTRYAG 2 cut(s) 132, 778
BstSLI GKGCMC 2 cut(s) 700, 787
BstSNI TACGTA 2 cut(s) 412, 722
BstV1I GCAGC 3 cut(s) 463, 559, 1055
BsuRI GGCC 3 cut(s) 5, 65, 1106
BtgZI GCGATG 1 cut(s) 777
BtsCI GGATG 1 cut(s) 1015
Cac8I GCNNGC 3 cut(s) 108, 296, 919
Cfr10I RCCGGY 1 cut(s) 734
Cfr13I GGNCC 2 cut(s) 1006, 1105
CseI GACGC 1 cut(s) 363
Csp6I GTAC 4 cut(s) 171, 409, 413, 1012
CviAII CATG 5 cut(s) 67, 80, 754, 947, 954
CviQI GTAC 4 cut(s) 171, 409, 413, 1012
DdeI CTNAG 1 cut(s) 481
DpnI GATC 3 cut(s) 385, 716, 984
DpnII GATC 3 cut(s) 383, 714, 982
EaeI YGGCCR 2 cut(s) 3, 63
Eam1104I CTCTTC 2 cut(s) 94, 284
EarI CTCTTC 2 cut(s) 94, 284
Eco105I TACGTA 2 cut(s) 412, 722
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 2 cut(s) 296, 753
Eco31I GGTCTC 1 cut(s) 786
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 1006
Eco88I CYCGRG 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 964
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 2 cut(s) 296, 753
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 5 cut(s) 70, 83, 757, 950, 957
FaqI GGGAC 2 cut(s) 402, 1019
FatI CATG 5 cut(s) 66, 79, 753, 946, 953
FauI CCCGC 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 452, 573, 1044
FokI GGATG 1 cut(s) 1002
FriOI GRGCYC 2 cut(s) 296, 753
Fsp4HI GCNGC 3 cut(s) 452, 573, 1044
FspBI CTAG 2 cut(s) 71, 662
GluI GCNGC 3 cut(s) 452, 573, 1044
HaeIII GGCC 3 cut(s) 5, 65, 1106
HapII CCGG 1 cut(s) 735
HgaI GACGC 1 cut(s) 363
Hin1II CATG 5 cut(s) 70, 83, 757, 950, 957
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 3 cut(s) 83, 128, 848
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 1 cut(s) 735
HphI GGTGA 1 cut(s) 1109
Hpy166II GTNNAC 5 cut(s) 21, 367, 409, 785, 959
Hpy188I TCNGA 7 cut(s) 319, 484, 714, 791, 817, 904, 970
Hpy188III TCNNGA 3 cut(s) 378, 387, 875
Hpy8I GTNNAC 5 cut(s) 21, 367, 409, 785, 959
Hpy99I CGWCG 2 cut(s) 257, 379
HpyAV CCTTC 2 cut(s) 340, 1118
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 3 cut(s) 252, 411, 721
HpyCH4V TGCA 7 cut(s) 110, 176, 451, 785, 802, 822, 839
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 950
HpyF3I CTNAG 1 cut(s) 481
HpySE526I ACGT 3 cut(s) 252, 411, 721
Hsp92II CATG 5 cut(s) 70, 83, 757, 950, 957
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 3 cut(s) 383, 714, 982
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 2 cut(s) 665, 748
Lsp1109I GCAGC 3 cut(s) 463, 559, 1055
LweI GCATC 1 cut(s) 754
MaeI CTAG 2 cut(s) 71, 662
MaeII ACGT 3 cut(s) 252, 411, 721
MaeIII GTNAC 3 cut(s) 245, 548, 1064
MalI GATC 3 cut(s) 385, 716, 984
MboI GATC 3 cut(s) 383, 714, 982
MboII GAAGA 6 cut(s) 81, 243, 301, 337, 684, 1026
MhlI GDGCHC 4 cut(s) 296, 700, 753, 787
MlsI TGGCCA 2 cut(s) 5, 65
MluNI TGGCCA 2 cut(s) 5, 65
MmeI TCCRAC 3 cut(s) 124, 523, 1043
MnlI CCTC 9 cut(s) 97, 200, 222, 374, 637, 806, 898, 943, 1021
Mox20I TGGCCA 2 cut(s) 5, 65
MscI TGGCCA 2 cut(s) 5, 65
MseI TTAA 3 cut(s) 366, 644, 909
MslI CAYNNNNRTG 1 cut(s) 703
Msp20I TGGCCA 2 cut(s) 5, 65
MspI CCGG 1 cut(s) 735
MwoI GCNNNNNNNGC 2 cut(s) 103, 950
NdeII GATC 3 cut(s) 383, 714, 982
NlaIII CATG 5 cut(s) 70, 83, 757, 950, 957
NlaIV GGNNCC 6 cut(s) 148, 351, 667, 750, 1007, 1083
NspI RCATGY 1 cut(s) 957
OliI CACNNNNGTG 1 cut(s) 703
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 882
PfeI GAWTC 3 cut(s) 83, 128, 848
PkrI GCNGC 3 cut(s) 453, 574, 1045
Ppu21I YACGTR 2 cut(s) 412, 722
PspN4I GGNNCC 6 cut(s) 148, 351, 667, 750, 1007, 1083
PspPI GGNCC 2 cut(s) 1006, 1105
RsaI GTAC 4 cut(s) 172, 410, 414, 1013
RsaNI GTAC 4 cut(s) 171, 409, 413, 1012
RseI CAYNNNNRTG 1 cut(s) 703
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 3 cut(s) 366, 644, 909
SatI GCNGC 3 cut(s) 452, 573, 1044
Sau3AI GATC 3 cut(s) 383, 714, 982
Sau96I GGNCC 2 cut(s) 1006, 1105
SduI GDGCHC 4 cut(s) 296, 700, 753, 787
SfaNI GCATC 1 cut(s) 754
SfcI CTRYAG 2 cut(s) 132, 778
SinI GGWCC 1 cut(s) 1006
SmiMI CAYNNNNRTG 1 cut(s) 703
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
SnaBI TACGTA 2 cut(s) 412, 722
SsiI CCGC 1 cut(s) 296
SspI AATATT 1 cut(s) 514
SspMI CTAG 2 cut(s) 71, 662
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 3 cut(s) 255, 414, 724
TaqI TCGA 1 cut(s) 876
TatI WGTACW 1 cut(s) 170
TfiI GAWTC 3 cut(s) 83, 128, 848
Tru1I TTAA 3 cut(s) 366, 644, 909
Tru9I TTAA 3 cut(s) 366, 644, 909
TseI GCWGC 3 cut(s) 451, 572, 1043
TspDTI ATGAA 4 cut(s) 96, 648, 770, 840
TspGWI ACGGA 1 cut(s) 484
VneI GTGCAC 1 cut(s) 783
VpaK11BI GGWCC 1 cut(s) 1006
XapI RAATTY 2 cut(s) 649, 964
XceI RCATGY 1 cut(s) 957
XspI CTAG 2 cut(s) 71, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.