MD15G1235600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000448
Physical Location & Seq
Forward (+)
19369453 .. 19372527
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1235600.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGTATATCATGGCAAAGAAAGCAAAGCTTTGTGGTCCATTATTTGCATTTTTGTTTCTATTTTCTTTGGGGTACAGAGCCCATGCAGATGATCCAGTGACAATTTTCATATTAGGAGACTCCACTGTGGATGTCGGCACCAACAATTGGTTACCAGAAAGTTACTGCAGGGCTGATTTCTCTCCCAATGGGGTTGATTTGCCTTATTCTGTGCCTACTGGGAGGTTCAGCAACGGCCTTAACAGCGCCGACCAAATCGCGAGTCTATTTGGCTACCAGAGAAGTCCGCCACCTTTCTTGTATATGATCAATCACCCAGCCACTTTTAAAAGGGATATACTGCGGGGGGTGAACTTTGCTTCAGCTGGAGCCGGCATATTCTACGAAACTGGCCAAAAACGATTTATCAAAGTTGTGCCACTGAGCGACCAGATTCAACAATTTGCGACGGTCAAAGGGAATTTGACAGAGTTGATAGGTCCCGTTGCAACTGCTAATGTGCTTGCCAAGTCTTTGTTCGTCGTTAGTATTGGAAGCAACGACCTTTTCGAGCATGTTCCTCCAAATTCCTCCACCTTAGTTGATCATAACGACGACTTGTACATCACAAATCTTCAGTTCACGTACCATGGTCATTTAGAGAGTTTATACAAGCTAGGAGCTCGCAAATTTGGGATTATGAGTATTCCTCCAATTGGATGCTGTCCTAAACTACGTGCTTCATCAAAGAAGCCGGGTGCTTGTAGAGAAGATCTAAACAAGCTCGCTCAATCCTTTTTCATTTCACTTGAGGATCTTCTGCGAAAAATGAGTTCAAAATTGGTAGGGATGAAATACTCACTCGCGAATACTTATGAAATGACCATGAGTATCATTGAAGACCCATTAGCCTTCGGCTTCAAGGACGTCCAATCAGCCTGCTGTGGATCAGGAAAGTTCAATGGAGAATTGCCATGCTTTTTTCTCCTTGGCCCCAACCTTTGTGCGAATCGGCACGAGTTCTTGTTCTGGGACAGTTATCATCCCACTGAATATGCTTCGAAATTGGCAGCACTTACCCTCTATGGTGGAGGAACAAGACTTGTCACACCCATGAATTTCAGTCGGTTGGTGGCTACTCATGTTAATATCACTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

380

Amino Acids

41.79

Weight (kDa)

8.47

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 351 1.2e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 909
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 147
AccII CGCG 2 cut(s) 260, 845
AciI CCGC 2 cut(s) 287, 343
AclWI GGATC 3 cut(s) 86, 801, 934
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 4 cut(s) 460, 565, 668, 1096
AcuI CTGAAG 2 cut(s) 345, 599
AcyI GRCGYC 1 cut(s) 906
AfaI GTAC 3 cut(s) 74, 602, 626
AfiI CCNNNNNNNGG 2 cut(s) 147, 695
AgsI TTSAA 5 cut(s) 437, 816, 878, 901, 940
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AluBI AGCT 5 cut(s) 28, 365, 655, 662, 763
AluI AGCT 5 cut(s) 28, 365, 655, 662, 763
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 3 cut(s) 86, 801, 934
AoxI GGCC 3 cut(s) 235, 391, 970
ApeKI GCWGC 1 cut(s) 1049
ApoI RAATTY 4 cut(s) 460, 565, 668, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 247, 279
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 3 cut(s) 35, 479, 971
AsuC2I CCSGG 1 cut(s) 735
AsuHPI GGTGA 2 cut(s) 305, 361
AsuII TTCGAA 1 cut(s) 1040
AvaII GGWCC 2 cut(s) 35, 479
BalI TGGCCA 1 cut(s) 393
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 2 cut(s) 82, 664
BauI CACGAG 1 cut(s) 995
BbsI GAAGAC 1 cut(s) 885
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 1 cut(s) 1061
BceAI ACGGC 1 cut(s) 250
BclI TGATCA 2 cut(s) 306, 583
BcnI CCSGG 1 cut(s) 735
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 1 cut(s) 656
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 249
BglII AGATCT 1 cut(s) 751
BisI GCNGC 1 cut(s) 1050
BlsI GCNGC 1 cut(s) 1051
Bme1390I CCNGG 1 cut(s) 735
Bme18I GGWCC 2 cut(s) 35, 479
BmgT120I GGNCC 3 cut(s) 35, 479, 971
BmiI GGNNCC 4 cut(s) 139, 370, 481, 973
BmrFI CCNGG 1 cut(s) 735
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 689
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 885
BplI GAGNNNNNCTC 2 cut(s) 673, 705
BpmI CTGGAG 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 1040
BpuEI CTTGAG 1 cut(s) 809
BpuMI CCSGG 1 cut(s) 735
BsaAI YACGTR 2 cut(s) 624, 716
BsaHI GRCGYC 1 cut(s) 906
BsaJI CCNNGG 2 cut(s) 628, 967
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 695
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 3 cut(s) 95, 223, 394
BseDI CCNNGG 2 cut(s) 628, 967
BseGI GGATG 4 cut(s) 136, 704, 834, 1021
BseLI CCNNNNNNNGG 2 cut(s) 147, 695
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 3 cut(s) 95, 223, 394
BseXI GCAGC 1 cut(s) 1061
BseYI CCCAGC 1 cut(s) 316
Bsh1236I CGCG 2 cut(s) 260, 845
BshFI GGCC 3 cut(s) 237, 393, 972
BshNI GGYRCC 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 2 cut(s) 372, 734
BslFI GGGAC 2 cut(s) 465, 1025
BslI CCNNNNNNNGG 2 cut(s) 147, 695
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 2 cut(s) 465, 1025
BsnI GGCC 3 cut(s) 237, 393, 972
Bsp119I TTCGAA 1 cut(s) 1040
Bsp1286I GDGCHC 2 cut(s) 82, 664
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 6 cut(s) 91, 306, 583, 751, 793, 926
Bsp19I CCATGG 1 cut(s) 628
Bsp68I TCGCGA 2 cut(s) 260, 845
BspACI CCGC 2 cut(s) 287, 343
BspANI GGCC 3 cut(s) 237, 393, 972
BspCNI CTCAG 1 cut(s) 414
BspFNI CGCG 2 cut(s) 260, 845
BspLI GGNNCC 4 cut(s) 139, 370, 481, 973
BspMAI CTGCAG 1 cut(s) 170
BspPI GGATC 3 cut(s) 86, 801, 934
BspT104I TTCGAA 1 cut(s) 1040
BspT107I GGYRCC 1 cut(s) 137
BsrFI RCCGGY 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 3 cut(s) 95, 223, 394
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 628, 967
BssMI GATC 6 cut(s) 91, 306, 583, 751, 793, 926
BssNI GRCGYC 1 cut(s) 906
BssSI CACGAG 1 cut(s) 995
BssT1I CCWWGG 2 cut(s) 628, 967
Bst2BI CACGAG 1 cut(s) 995
Bst4CI ACNGT 3 cut(s) 127, 451, 1016
BstACI GRCGYC 1 cut(s) 906
BstAUI TGTACA 1 cut(s) 600
BstBAI YACGTR 2 cut(s) 624, 716
BstBI TTCGAA 1 cut(s) 1040
BstC8I GCNNGC 5 cut(s) 373, 504, 664, 765, 919
BstDEI CTNAG 2 cut(s) 422, 577
BstDSI CCRYGG 1 cut(s) 628
BstEII GGTNACC 1 cut(s) 150
BstF5I GGATG 4 cut(s) 136, 704, 834, 1021
BstFNI CGCG 2 cut(s) 260, 845
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 6 cut(s) 94, 309, 586, 754, 796, 929
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 6 cut(s) 91, 306, 583, 751, 793, 926
BstMWI GCNNNNNNNGC 2 cut(s) 20, 243
BstNSI RCATGY 1 cut(s) 557
BstPI GGTNACC 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 733
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 2 cut(s) 260, 845
BstV1I GCAGC 1 cut(s) 1061
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 2 cut(s) 751, 793
BstYI RGATCY 2 cut(s) 751, 793
BsuRI GGCC 3 cut(s) 237, 393, 972
BtgI CCRYGG 1 cut(s) 628
BtsCI GGATG 4 cut(s) 136, 704, 834, 1021
BtsIMutI CAGTG 4 cut(s) 102, 123, 419, 1026
BtuMI TCGCGA 2 cut(s) 260, 845
Cac8I GCNNGC 5 cut(s) 373, 504, 664, 765, 919
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 3 cut(s) 35, 479, 971
Csp6I GTAC 3 cut(s) 73, 601, 625
CspCI CAANNNNNGTGG 4 cut(s) 279, 314, 562, 597
CviAII CATG 8 cut(s) 10, 83, 554, 629, 865, 954, 1093, 1121
CviQI GTAC 3 cut(s) 73, 601, 625
DdeI CTNAG 2 cut(s) 422, 577
DpnI GATC 6 cut(s) 93, 308, 585, 753, 795, 928
DpnII GATC 6 cut(s) 91, 306, 583, 751, 793, 926
DraI TTTAAA 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 391
EciI GGCGGA 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 662
Eco130I CCWWGG 2 cut(s) 628, 967
Eco24I GRGCYC 2 cut(s) 82, 664
Eco47I GGWCC 2 cut(s) 35, 479
Eco53kI GAGCTC 1 cut(s) 662
Eco57I CTGAAG 2 cut(s) 345, 599
Eco91I GGTNACC 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 662
EcoO109I RGGNCCY 1 cut(s) 479
EcoO65I GGTNACC 1 cut(s) 150
EcoT14I CCWWGG 2 cut(s) 628, 967
EcoT38I GRGCYC 2 cut(s) 82, 664
ErhI CCWWGG 2 cut(s) 628, 967
FaeI CATG 8 cut(s) 13, 86, 557, 632, 868, 957, 1096, 1124
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 2 cut(s) 465, 1025
FatI CATG 8 cut(s) 9, 82, 553, 628, 864, 953, 1092, 1120
FauI CCCGC 1 cut(s) 336
FbaI TGATCA 2 cut(s) 306, 583
Fnu4HI GCNGC 1 cut(s) 1050
FokI GGATG 4 cut(s) 143, 711, 841, 1008
FriOI GRGCYC 2 cut(s) 82, 664
Fsp4HI GCNGC 1 cut(s) 1050
FspBI CTAG 1 cut(s) 656
GlaI GCGC 1 cut(s) 247
GluI GCNGC 1 cut(s) 1050
GsaI CCCAGC 1 cut(s) 320
GsuI CTGGAG 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 3 cut(s) 237, 393, 972
HapII CCGG 2 cut(s) 372, 734
HhaI GCGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 906
Hin1II CATG 8 cut(s) 13, 86, 557, 632, 868, 957, 1096, 1124
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 4 cut(s) 119, 262, 433, 988
HpaII CCGG 2 cut(s) 372, 734
HphI GGTGA 2 cut(s) 305, 361
Hpy166II GTNNAC 2 cut(s) 352, 621
Hpy188III TCNNGA 3 cut(s) 259, 844, 930
Hpy8I GTNNAC 2 cut(s) 352, 621
Hpy99I CGWCG 3 cut(s) 451, 524, 596
HpyAV CCTTC 1 cut(s) 901
HpyCH4III ACNGT 3 cut(s) 127, 451, 1016
HpyCH4IV ACGT 3 cut(s) 623, 715, 906
HpyCH4V TGCA 4 cut(s) 47, 86, 168, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 243
HpyF3I CTNAG 2 cut(s) 422, 577
HpySE526I ACGT 3 cut(s) 623, 715, 906
Hsp92I GRCGYC 1 cut(s) 906
Hsp92II CATG 8 cut(s) 13, 86, 557, 632, 868, 957, 1096, 1124
HspAI GCGC 1 cut(s) 246
KroI GCCGGC 1 cut(s) 371
KroNI GCCGGC 1 cut(s) 373
Ksp22I TGATCA 2 cut(s) 306, 583
Kzo9I GATC 6 cut(s) 91, 306, 583, 751, 793, 926
LmnI GCTCC 2 cut(s) 368, 659
Lsp1109I GCAGC 1 cut(s) 1061
LweI GCATC 1 cut(s) 689
MaeI CTAG 1 cut(s) 656
MaeII ACGT 3 cut(s) 623, 715, 906
MaeIII GTNAC 4 cut(s) 97, 150, 161, 1084
MalI GATC 6 cut(s) 93, 308, 585, 753, 795, 928
MboI GATC 6 cut(s) 91, 306, 583, 751, 793, 926
MboII GAAGA 4 cut(s) 605, 761, 788, 890
MfeI CAATTG 2 cut(s) 145, 693
MflI RGATCY 2 cut(s) 751, 793
MhlI GDGCHC 2 cut(s) 82, 664
MlsI TGGCCA 1 cut(s) 393
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 2 cut(s) 113, 271
MnlI CCTC 7 cut(s) 216, 570, 580, 699, 784, 1064, 1070
Mox20I TGGCCA 1 cut(s) 393
MroNI GCCGGC 1 cut(s) 371
MscI TGGCCA 1 cut(s) 393
MseI TTAA 3 cut(s) 240, 327, 1125
MslI CAYNNNNRTG 2 cut(s) 87, 1091
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 2 cut(s) 372, 734
MspR9I CCNGG 1 cut(s) 735
MunI CAATTG 2 cut(s) 145, 693
MvnI CGCG 2 cut(s) 260, 845
MwoI GCNNNNNNNGC 2 cut(s) 20, 243
NaeI GCCGGC 1 cut(s) 373
NciI CCSGG 1 cut(s) 735
NcoI CCATGG 1 cut(s) 628
NdeII GATC 6 cut(s) 91, 306, 583, 751, 793, 926
NgoMIV GCCGGC 1 cut(s) 371
NlaIII CATG 8 cut(s) 13, 86, 557, 632, 868, 957, 1096, 1124
NlaIV GGNNCC 4 cut(s) 139, 370, 481, 973
NmuCI GTSAC 2 cut(s) 97, 1084
NruI TCGCGA 2 cut(s) 260, 845
NspI RCATGY 1 cut(s) 557
NspV TTCGAA 1 cut(s) 1040
PcsI WCGNNNNNNNCGW 1 cut(s) 546
PdiI GCCGGC 1 cut(s) 373
PfeI GAWTC 2 cut(s) 433, 988
PflMI CCANNNNNTGG 1 cut(s) 147
PkrI GCNGC 1 cut(s) 1051
PleI GAGTC 2 cut(s) 113, 270
PpsI GAGTC 2 cut(s) 113, 270
Ppu21I YACGTR 2 cut(s) 624, 716
PpuMI RGGWCCY 1 cut(s) 479
Psp124BI GAGCTC 1 cut(s) 664
Psp5II RGGWCCY 1 cut(s) 479
PspEI GGTNACC 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 316
PspN4I GGNNCC 4 cut(s) 139, 370, 481, 973
PspPI GGNCC 3 cut(s) 35, 479, 971
PspPPI RGGWCCY 1 cut(s) 479
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 2 cut(s) 751, 793
PvuII CAGCTG 1 cut(s) 365
RruI TCGCGA 2 cut(s) 260, 845
RsaI GTAC 3 cut(s) 74, 602, 626
RsaNI GTAC 3 cut(s) 73, 601, 625
RseI CAYNNNNRTG 2 cut(s) 87, 1091
SacI GAGCTC 1 cut(s) 664
SaqAI TTAA 3 cut(s) 240, 327, 1125
SatI GCNGC 1 cut(s) 1050
Sau3AI GATC 6 cut(s) 91, 306, 583, 751, 793, 926
Sau96I GGNCC 3 cut(s) 35, 479, 971
SchI GAGTC 2 cut(s) 113, 271
ScrFI CCNGG 1 cut(s) 735
SduI GDGCHC 2 cut(s) 82, 664
SfaNI GCATC 1 cut(s) 689
SfcI CTRYAG 1 cut(s) 166
SfuI TTCGAA 1 cut(s) 1040
SinI GGWCC 2 cut(s) 35, 479
SmiMI CAYNNNNRTG 2 cut(s) 87, 1091
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
SsiI CCGC 2 cut(s) 287, 343
SspMI CTAG 1 cut(s) 656
SstI GAGCTC 1 cut(s) 664
StyD4I CCNGG 1 cut(s) 733
StyI CCWWGG 2 cut(s) 628, 967
TaaI ACNGT 3 cut(s) 127, 451, 1016
TaiI ACGT 3 cut(s) 626, 718, 909
TaqI TCGA 2 cut(s) 549, 1040
TatI WGTACW 1 cut(s) 600
TfiI GAWTC 2 cut(s) 433, 988
Tru1I TTAA 3 cut(s) 240, 327, 1125
Tru9I TTAA 3 cut(s) 240, 327, 1125
TscAI CASTG 4 cut(s) 102, 130, 426, 1033
TseFI GTSAC 2 cut(s) 97, 1084
TseI GCWGC 1 cut(s) 1049
Tsp45I GTSAC 2 cut(s) 97, 1084
TspDTI ATGAA 6 cut(s) 97, 711, 769, 845, 870, 1109
TspRI CASTG 4 cut(s) 102, 130, 426, 1033
Van91I CCANNNNNTGG 1 cut(s) 147
VpaK11BI GGWCC 2 cut(s) 35, 479
XapI RAATTY 4 cut(s) 460, 565, 668, 1096
XceI RCATGY 1 cut(s) 557
XspI CTAG 1 cut(s) 656
ZraI GACGTC 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.