RchiOBHm_Chr2g0098021

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000448
Physical Location & Seq
Forward (+)
10496933 .. 10503363
6431 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47284

Sequence Viewer

Length: 2064 bp
ATGGCCAATAACAATGGGTGGACACCCTTGTTTGCTGTTTGGCTTTTTGTGTTTGTATATCTGTCCATGCTAGGTTTCCATGCATCCTCTTCACAGCAAATTGTGCCTGCAATTTACATATTTGGAGATTCTCCAGGACATGTTGGAACCAATAATTACTTGCCTGATTGTACTGCAAGGGCTGATTACCTCTATAATGGGATTGACTACCCTCACTCTAAACCAACAGGAAGATTTAGCAACGGTTACAACGTCGTAGATTACATCGAGACCTTTGGCTACGCCACTGCCCACCACTACACTAGAATTTCCCACAAGGAGAGCCCGCCACCTTTTCTCTCTCTTTTAGAAGAGAAGAAGCAATTTCCTAACAGGAAGGTTCCAAATAAGGGAGTTAACTTTGCGTCAGGAGGATCAGGACTTCTGGATGATACTGGTAAACTACAGTGGGGAAATGTCGAGATCATTTCCTTGGGGGAACAAGTGCAGCAATTCCAAACTTTACGGAACAAAATCTCAGAATTATTGGGTGCTGGTGGATTGGCTAATATTTCTCAGTCTCTCTTTGTCATCAGCGTTGGAAGCAACGACATCTTTGAATTATTCGCTGCTAATAACCTGACTAAAAGAACCGAGCAAGAGTACTTGAGCATACTCATCTCCTCTTACGAAACTCTAAATAAGGGTTGGACACTGCTAAACTTATTTATGCTCGGAGCAAGGAAGTTTGGGATTATCAGCGTCTCACAAATTGGGTGCCGCCCTGGCCAGCGAAAGCTCAGTGAAAGAGGCGGGTATTCGGATTTGTGTGAAAATCGCCGTAGCTACTTGTTCTGGGATCAATACCATTCAACAGAGGCTGCATCTGGAATCCTAGCCATATATCTGTTTGGTGGTGCTGCAGTTTTGCTCAGAGTATCAGCAACACTAGCCAGCTTAGCTAGTACAATTACCATGGCCAAGTCTGCAATGTTTTCTCTTTGGCTCAATATTATTGTGTATCTCGGCATACTTGGATTGCAAGTCTCACACTCTCAGCCATCTGTGCCAGCACTTTACATATTTGGTGACTCCACAGCTGATGTTGGAACCAACAATTATTTGCCCCGTTCTAGGGCGCGGGCCGATTTCCCTTATAACGGGATTGATTTTCCTCAATCTAGACCAACTGGGAGATTTAGCAACGGTTTCAACACTATTGATTATCTAGCCCAGTATCTGAAATTCTATGAAAGCCCGCCGCCTTTTCTCTCTTTTTCGGACAGCAATATTGCGAAATTTCTCATCAAGACGAAGGGAATCAATTTTGCTTCAGGAGGGTCAGGACTTCTGTATTCTACAGGACAAAAGAAGTATAGAAGGGTGATTTCTTTTGGAGAACAAGTCCAACAATTTGCAACTGTTCGCAGCCATATTTCAGAATTATTGGGTGCGAGTGCAGTCGCTAATATCTCCAAGTCTCTCTTCATCATCAGCGTCGGAAGCAATGACATATTCGAACATTTTGATTATAACTACACTAGTAACCCTATTGTTATTGGGCAAAACTACATCGCCACTCTCATCTCCACCTACGAAACTCATCTAAGGAATTTATATGAGCTAGGAGCCAGAAGATTTGGGATTATTAGTGTTGGGGCAATTGGGTGCTGTCCGTGTCAACGTAATTCGAGTAGATCTGGTGATTGTTTCGAGGCCATGAATGTGGATGCTCAGTTGTTCTACACTGCACTCCTACGTCTCTTGCAAAAGTTGAGTTCAGAGTGTGAGGGGTTGATGTATGCACTTGGAGATTCATACAAAATGACAAAATATATTATCGACAACCCCGATCAGTCTGTTTTTACCGAGATGAAAAAAGCTTGCTGTGGAAAAGGACAGTTGAAAGCAAAGCACGCATGTAAACAGGATTCGGATCTGTGTGAAAATCGCACAACCCACTTGTTTTGGGACCGGTACCATCCAACACAGACCGCTTCTAATCTCGCAGCCTTTGGTCTTTATAATGGTGACAAATCATCCGTGGTGCCCATGAGTTTTGCTGAATTGGCCCTTCGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

687

Amino Acids

76.12

Weight (kDa)

8.61

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 265 1.9e-11 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL PF00657 353 - 664 2.9e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1137, 1512, 2004
Acc65I GGTACC 1 cut(s) 1956
AccB1I GGYRCC 3 cut(s) 756, 1956, 2026
AccII CGCG 1 cut(s) 1120
AciI CCGC 7 cut(s) 326, 760, 792, 1120, 1238, 1241, 1974
AclWI GGATC 3 cut(s) 421, 846, 1923
AcoI YGGCCR 4 cut(s) 3, 766, 957, 2057
AcsI RAATTY 4 cut(s) 306, 1223, 1277, 1591
AcuI CTGAAG 1 cut(s) 1296
AfaI GTAC 4 cut(s) 172, 644, 946, 1958
AfiI CCNNNNNNNGG 4 cut(s) 389, 1113, 1114, 1139
AflIII ACRYGT 1 cut(s) 139
AgeI ACCGGT 1 cut(s) 1953
AgsI TTSAA 4 cut(s) 599, 852, 1192, 1885
AhlI ACTAGT 1 cut(s) 1520
AjnI CCWGG 2 cut(s) 133, 763
AjuI GAANNNNNNNTTGG 2 cut(s) 1448, 1480
AluBI AGCT 7 cut(s) 778, 825, 936, 941, 1079, 1603, 1862
AluI AGCT 7 cut(s) 778, 825, 936, 941, 1079, 1603, 1862
Alw26I GTCTC 6 cut(s) 263, 564, 748, 1030, 1464, 1745
AlwI GGATC 3 cut(s) 421, 846, 1923
AlwNI CAGNNNCTG 2 cut(s) 860, 1219
AoxI GGCC 7 cut(s) 3, 766, 957, 1122, 1695, 2049, 2057
ApeKI GCWGC 6 cut(s) 487, 608, 860, 899, 1407, 1988
ApoI RAATTY 4 cut(s) 306, 1223, 1277, 1591
AsiGI ACCGGT 1 cut(s) 1953
Asp718I GGTACC 1 cut(s) 1956
AspLEI GCGC 1 cut(s) 1120
AspS9I GGNCC 3 cut(s) 1122, 1951, 2050
AsuHPI GGTGA 4 cut(s) 1079, 1375, 1694, 2021
AsuII TTCGAA 1 cut(s) 1497
AvaII GGWCC 1 cut(s) 1951
BaeGI GKGCMC 1 cut(s) 2031
BalI TGGCCA 3 cut(s) 5, 768, 959
BanI GGYRCC 3 cut(s) 756, 1956, 2026
BanII GRGCYC 1 cut(s) 326
BbvI GCAGC 6 cut(s) 499, 595, 847, 886, 1419, 2000
BccI CCATC 2 cut(s) 1048, 1968
BceAI ACGGC 1 cut(s) 804
BciT130I CCWGG 2 cut(s) 135, 765
BcoDI GTCTC 6 cut(s) 263, 564, 748, 1030, 1464, 1745
BcuI ACTAGT 1 cut(s) 1520
BfmI CTRYAG 3 cut(s) 443, 900, 1338
BglI GCCNNNNNGGC 1 cut(s) 765
BglII AGATCT 1 cut(s) 1676
BisI GCNGC 8 cut(s) 488, 609, 760, 861, 900, 1241, 1408, 1989
BlpI GCTNAGC 1 cut(s) 937
BlsI GCNGC 8 cut(s) 489, 610, 761, 862, 901, 1242, 1409, 1990
BmcAI AGTACT 1 cut(s) 644
Bme1390I CCNGG 2 cut(s) 135, 765
Bme18I GGWCC 1 cut(s) 1951
BmgT120I GGNCC 3 cut(s) 1122, 1951, 2050
BmiI GGNNCC 8 cut(s) 148, 381, 758, 1090, 1609, 1952, 1958, 2028
BmrFI CCNGG 2 cut(s) 135, 765
BmrI ACTGGG 2 cut(s) 1179, 1207
BmsI GCATC 3 cut(s) 92, 872, 1699
BmuI ACTGGG 2 cut(s) 1179, 1207
BpmI CTGGAG 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 937
Bpu14I TTCGAA 1 cut(s) 1497
BpuEI CTTGAG 1 cut(s) 667
BsaBI GATNNNNATC 1 cut(s) 1914
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 4 cut(s) 471, 763, 954, 2022
BsaWI WCCGGW 1 cut(s) 1953
BsaXI ACNNNNNCTCC 2 cut(s) 1368, 1398
Bsc4I CCNNNNNNNGG 4 cut(s) 389, 1113, 1114, 1139
Bse118I RCCGGY 1 cut(s) 1953
Bse1I ACTGG 3 cut(s) 439, 1174, 1213
Bse3DI GCAATG 2 cut(s) 975, 1492
Bse8I GATNNNNATC 1 cut(s) 1914
BseBI CCWGG 2 cut(s) 135, 765
BseDI CCNNGG 4 cut(s) 471, 763, 954, 2022
BseGI GGATG 5 cut(s) 83, 433, 1714, 1960, 2018
BseJI GATNNNNATC 1 cut(s) 1914
BseLI CCNNNNNNNGG 4 cut(s) 389, 1113, 1114, 1139
BseMI GCAATG 2 cut(s) 975, 1492
BseMII CTCAG 6 cut(s) 531, 569, 793, 925, 1049, 1727
BseNI ACTGG 3 cut(s) 439, 1174, 1213
BseRI GAGGAG 1 cut(s) 652
BseSI GKGCMC 1 cut(s) 2031
BseXI GCAGC 6 cut(s) 499, 595, 847, 886, 1419, 2000
BsgI GTGCAG 3 cut(s) 506, 1458, 1713
Bsh1236I CGCG 1 cut(s) 1120
BshFI GGCC 7 cut(s) 5, 768, 959, 1124, 1697, 2051, 2059
BshNI GGYRCC 3 cut(s) 756, 1956, 2026
BshTI ACCGGT 1 cut(s) 1953
BsiSI CCGG 1 cut(s) 1954
BslFI GGGAC 1 cut(s) 1964
BslI CCNNNNNNNGG 4 cut(s) 389, 1113, 1114, 1139
BsmAI GTCTC 6 cut(s) 263, 564, 748, 1030, 1464, 1745
BsmBI CGTCTC 2 cut(s) 748, 1745
BsmFI GGGAC 1 cut(s) 1964
BsnI GGCC 7 cut(s) 5, 768, 959, 1124, 1697, 2051, 2059
Bso31I GGTCTC 1 cut(s) 263
Bsp119I TTCGAA 1 cut(s) 1497
Bsp1286I GDGCHC 2 cut(s) 326, 2031
Bsp143I GATC 6 cut(s) 413, 462, 838, 1676, 1831, 1915
Bsp1720I GCTNAGC 1 cut(s) 937
Bsp19I CCATGG 1 cut(s) 954
BspACI CCGC 7 cut(s) 326, 760, 792, 1120, 1238, 1241, 1974
BspANI GGCC 7 cut(s) 5, 768, 959, 1124, 1697, 2051, 2059
BspCNI CTCAG 6 cut(s) 530, 568, 792, 924, 1048, 1726
BspFNI CGCG 1 cut(s) 1120
BspLI GGNNCC 8 cut(s) 148, 381, 758, 1090, 1609, 1952, 1958, 2028
BspMAI CTGCAG 1 cut(s) 904
BspPI GGATC 3 cut(s) 421, 846, 1923
BspT104I TTCGAA 1 cut(s) 1497
BspT107I GGYRCC 3 cut(s) 756, 1956, 2026
BspTNI GGTCTC 1 cut(s) 263
BsrDI GCAATG 2 cut(s) 975, 1492
BsrFI RCCGGY 1 cut(s) 1953
BsrI ACTGG 3 cut(s) 439, 1174, 1213
BssAI RCCGGY 1 cut(s) 1953
BssECI CCNNGG 4 cut(s) 471, 763, 954, 2022
BssMI GATC 6 cut(s) 413, 462, 838, 1676, 1831, 1915
BssT1I CCWWGG 2 cut(s) 471, 954
Bst2UI CCWGG 2 cut(s) 135, 765
Bst4CI ACNGT 5 cut(s) 245, 447, 1187, 1402, 1881
Bst6I CTCTTC 3 cut(s) 94, 345, 1469
BstAPI GCANNNNNTGC 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 1497
BstC8I GCNNGC 9 cut(s) 108, 326, 770, 934, 1050, 1122, 1238, 1864, 1896
BstDEI CTNAG 8 cut(s) 517, 555, 779, 911, 937, 1035, 1586, 1713
BstDSI CCRYGG 2 cut(s) 954, 2022
BstF5I GGATG 5 cut(s) 83, 433, 1714, 1960, 2018
BstFNI CGCG 1 cut(s) 1120
BstHHI GCGC 1 cut(s) 1120
BstKTI GATC 6 cut(s) 416, 465, 841, 1679, 1834, 1918
BstMAI GTCTC 6 cut(s) 263, 564, 748, 1030, 1464, 1745
BstMBI GATC 6 cut(s) 413, 462, 838, 1676, 1831, 1915
BstNI CCWGG 2 cut(s) 135, 765
BstNSI RCATGY 2 cut(s) 143, 1902
BstSCI CCNGG 2 cut(s) 133, 763
BstSFI CTRYAG 3 cut(s) 443, 900, 1338
BstSLI GKGCMC 1 cut(s) 2031
BstUI CGCG 1 cut(s) 1120
BstV1I GCAGC 6 cut(s) 499, 595, 847, 886, 1419, 2000
BstX2I RGATCY 2 cut(s) 1676, 1915
BstXI CCANNNNNNTGG 1 cut(s) 1705
BstYI RGATCY 2 cut(s) 1676, 1915
BsuRI GGCC 7 cut(s) 5, 768, 959, 1124, 1697, 2051, 2059
BtgI CCRYGG 2 cut(s) 954, 2022
BtgZI GCGATG 1 cut(s) 1537
BtsCI GGATG 5 cut(s) 83, 433, 1714, 1960, 2018
BtsI GCAGTG 3 cut(s) 285, 692, 1725
BtsIMutI CAGTG 5 cut(s) 285, 452, 692, 787, 1725
Cac8I GCNNGC 9 cut(s) 108, 326, 770, 934, 1050, 1122, 1238, 1864, 1896
CaiI CAGNNNCTG 2 cut(s) 860, 1219
CfoI GCGC 1 cut(s) 1120
Cfr10I RCCGGY 1 cut(s) 1953
Cfr13I GGNCC 3 cut(s) 1122, 1951, 2050
CseI GACGC 3 cut(s) 393, 730, 1465
Csp6I GTAC 4 cut(s) 171, 643, 945, 1957
CspAI ACCGGT 1 cut(s) 1953
CspCI CAANNNNNGTGG 2 cut(s) 1928, 1963
CviAII CATG 8 cut(s) 67, 80, 140, 955, 1699, 1899, 2032, 2061
CviQI GTAC 4 cut(s) 171, 643, 945, 1957
DdeI CTNAG 8 cut(s) 517, 555, 779, 911, 937, 1035, 1586, 1713
DpnI GATC 6 cut(s) 415, 464, 840, 1678, 1833, 1917
DpnII GATC 6 cut(s) 413, 462, 838, 1676, 1831, 1915
EaeI YGGCCR 4 cut(s) 3, 766, 957, 2057
Eam1104I CTCTTC 3 cut(s) 94, 345, 1469
EarI CTCTTC 3 cut(s) 94, 345, 1469
Eco130I CCWWGG 2 cut(s) 471, 954
Eco24I GRGCYC 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 1 cut(s) 1951
Eco57I CTGAAG 1 cut(s) 1296
EcoRII CCWGG 2 cut(s) 133, 763
EcoT14I CCWWGG 2 cut(s) 471, 954
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 326
ErhI CCWWGG 2 cut(s) 471, 954
Esp3I CGTCTC 2 cut(s) 748, 1745
FaeI CATG 8 cut(s) 70, 83, 143, 958, 1702, 1902, 2035, 2064
FalI AAGNNNNNCTT 2 cut(s) 1448, 1480
FaqI GGGAC 1 cut(s) 1964
FatI CATG 8 cut(s) 66, 79, 139, 954, 1698, 1898, 2031, 2060
FauI CCCGC 4 cut(s) 333, 785, 1113, 1245
Fnu4HI GCNGC 8 cut(s) 488, 609, 760, 861, 900, 1241, 1408, 1989
FokI GGATG 5 cut(s) 70, 440, 1721, 1947, 2005
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 8 cut(s) 488, 609, 760, 861, 900, 1241, 1408, 1989
GlaI GCGC 1 cut(s) 1119
GluI GCNGC 8 cut(s) 488, 609, 760, 861, 900, 1241, 1408, 1989
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 7 cut(s) 5, 768, 959, 1124, 1697, 2051, 2059
HapII CCGG 1 cut(s) 1954
HgaI GACGC 3 cut(s) 393, 730, 1465
HhaI GCGC 1 cut(s) 1120
Hin1II CATG 8 cut(s) 70, 83, 143, 958, 1702, 1902, 2035, 2064
Hin6I GCGC 1 cut(s) 1118
HinP1I GCGC 1 cut(s) 1118
HincII GTYRAC 2 cut(s) 397, 1661
HindII GTYRAC 2 cut(s) 397, 1661
HindIII AAGCTT 1 cut(s) 1860
HinfI GANTC 6 cut(s) 128, 870, 1070, 1299, 1793, 1910
HpaI GTTAAC 1 cut(s) 397
HpaII CCGG 1 cut(s) 1954
HphI GGTGA 4 cut(s) 1079, 1375, 1694, 2021
Hpy166II GTNNAC 5 cut(s) 21, 397, 440, 1661, 1904
Hpy8I GTNNAC 5 cut(s) 21, 397, 440, 1661, 1904
Hpy99I CGWCG 2 cut(s) 257, 1481
HpyAV CCTTC 4 cut(s) 370, 1288, 1353, 2063
HpyCH4III ACNGT 5 cut(s) 245, 447, 1187, 1402, 1881
HpyCH4IV ACGT 3 cut(s) 252, 1663, 1738
HpyF3I CTNAG 8 cut(s) 517, 555, 779, 911, 937, 1035, 1586, 1713
HpySE526I ACGT 3 cut(s) 252, 1663, 1738
Hsp92II CATG 8 cut(s) 70, 83, 143, 958, 1702, 1902, 2035, 2064
HspAI GCGC 1 cut(s) 1118
KpnI GGTACC 1 cut(s) 1960
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 6 cut(s) 413, 462, 838, 1676, 1831, 1915
LmnI GCTCC 2 cut(s) 716, 1607
Lsp1109I GCAGC 6 cut(s) 499, 595, 847, 886, 1419, 2000
LweI GCATC 3 cut(s) 92, 872, 1699
MaeII ACGT 3 cut(s) 252, 1663, 1738
MaeIII GTNAC 4 cut(s) 245, 1067, 1523, 2009
MalI GATC 6 cut(s) 415, 464, 840, 1678, 1833, 1917
MboI GATC 6 cut(s) 413, 462, 838, 1676, 1831, 1915
MboII GAAGA 6 cut(s) 81, 243, 362, 367, 1456, 1626
MfeI CAATTG 1 cut(s) 1641
MflI RGATCY 2 cut(s) 1676, 1915
MhlI GDGCHC 2 cut(s) 326, 2031
MlsI TGGCCA 3 cut(s) 5, 768, 959
MluNI TGGCCA 3 cut(s) 5, 768, 959
MlyI GAGTC 1 cut(s) 1064
MmeI TCCRAC 7 cut(s) 124, 559, 668, 1066, 1411, 1459, 1988
Mox20I TGGCCA 3 cut(s) 5, 768, 959
Mph1103I ATGCAT 1 cut(s) 85
MscI TGGCCA 3 cut(s) 5, 768, 959
MseI TTAA 1 cut(s) 396
MslI CAYNNNNRTG 1 cut(s) 1703
Msp20I TGGCCA 3 cut(s) 5, 768, 959
MspA1I CMGCKG 1 cut(s) 1079
MspI CCGG 1 cut(s) 1954
MspR9I CCNGG 2 cut(s) 135, 765
MunI CAATTG 1 cut(s) 1641
MvaI CCWGG 2 cut(s) 135, 765
MvnI CGCG 1 cut(s) 1120
NcoI CCATGG 1 cut(s) 954
NdeII GATC 6 cut(s) 413, 462, 838, 1676, 1831, 1915
NlaIII CATG 8 cut(s) 70, 83, 143, 958, 1702, 1902, 2035, 2064
NlaIV GGNNCC 8 cut(s) 148, 381, 758, 1090, 1609, 1952, 1958, 2028
NmeAIII GCCGAG 1 cut(s) 984
NmuCI GTSAC 2 cut(s) 1067, 2009
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 2 cut(s) 143, 1902
NspV TTCGAA 1 cut(s) 1497
PciI ACATGT 1 cut(s) 139
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 1827
PfeI GAWTC 5 cut(s) 128, 870, 1299, 1793, 1910
PfoI TCCNGGA 1 cut(s) 133
PinAI ACCGGT 1 cut(s) 1953
PkrI GCNGC 8 cut(s) 489, 610, 761, 862, 901, 1242, 1409, 1990
PleI GAGTC 1 cut(s) 1064
PpsI GAGTC 1 cut(s) 1064
PscI ACATGT 1 cut(s) 139
PsiI TTATAA 3 cut(s) 1137, 1512, 2004
Psp6I CCWGG 2 cut(s) 133, 763
PspGI CCWGG 2 cut(s) 133, 763
PspN4I GGNNCC 8 cut(s) 148, 381, 758, 1090, 1609, 1952, 1958, 2028
PspPI GGNCC 3 cut(s) 1122, 1951, 2050
PstI CTGCAG 1 cut(s) 904
PstNI CAGNNNCTG 2 cut(s) 860, 1219
PsuI RGATCY 2 cut(s) 1676, 1915
PvuII CAGCTG 1 cut(s) 1079
RsaI GTAC 4 cut(s) 172, 644, 946, 1958
RsaNI GTAC 4 cut(s) 171, 643, 945, 1957
RseI CAYNNNNRTG 1 cut(s) 1703
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 8 cut(s) 488, 609, 760, 861, 900, 1241, 1408, 1989
Sau3AI GATC 6 cut(s) 413, 462, 838, 1676, 1831, 1915
Sau96I GGNCC 3 cut(s) 1122, 1951, 2050
ScaI AGTACT 1 cut(s) 644
SchI GAGTC 1 cut(s) 1064
ScrFI CCNGG 2 cut(s) 135, 765
SduI GDGCHC 2 cut(s) 326, 2031
SfaNI GCATC 3 cut(s) 92, 872, 1699
SfcI CTRYAG 3 cut(s) 443, 900, 1338
SfuI TTCGAA 1 cut(s) 1497
SinI GGWCC 1 cut(s) 1951
SmiMI CAYNNNNRTG 1 cut(s) 1703
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SpeI ACTAGT 1 cut(s) 1520
SsiI CCGC 7 cut(s) 326, 760, 792, 1120, 1238, 1241, 1974
SspI AATATT 3 cut(s) 550, 991, 1270
StyD4I CCNGG 2 cut(s) 133, 763
StyI CCWWGG 2 cut(s) 471, 954
TaaI ACNGT 5 cut(s) 245, 447, 1187, 1402, 1881
TaiI ACGT 3 cut(s) 255, 1666, 1741
TaqI TCGA 6 cut(s) 267, 459, 1497, 1670, 1692, 1821
TatI WGTACW 3 cut(s) 170, 642, 944
TauI GCSGC 2 cut(s) 762, 1243
TfiI GAWTC 5 cut(s) 128, 870, 1299, 1793, 1910
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 5 cut(s) 292, 452, 699, 787, 1732
TseFI GTSAC 2 cut(s) 1067, 2009
TseI GCWGC 6 cut(s) 487, 608, 860, 899, 1407, 1988
Tsp45I GTSAC 2 cut(s) 1067, 2009
TspDTI ATGAA 5 cut(s) 1245, 1456, 1715, 1785, 1868
TspGWI ACGGA 3 cut(s) 520, 1644, 2011
TspRI CASTG 5 cut(s) 292, 452, 699, 787, 1732
VpaK11BI GGWCC 1 cut(s) 1951
XapI RAATTY 4 cut(s) 306, 1223, 1277, 1591
XbaI TCTAGA 1 cut(s) 1160
XceI RCATGY 2 cut(s) 143, 1902
ZrmI AGTACT 1 cut(s) 644
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.