Rroxscaffold_7G00188840

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000448
Physical Location & Seq
Forward (+)
28599433 .. 28601213
1781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00188840.1

Sequence Viewer

Length: 510 bp
ATGGAGAACAAATGGTTTCTTCCTCTAGTCCTATCCTTGTTTATGGCTGTTCTTAGTTTAGCAGCAGCAGCTAAGCCAGTACTATTGCCACCAATTTTCATACTAGGTGATTCAACTGCTGATGTAGGAACCAATAACTTCTTGCCAGGTCGCAAGGCAAGGGCAGACTTTCCCCACAATGGCATTGACTTTCCTAACTCACCAAGACCCACTGGAAGGTTCAGCAATGGCCTCAACAGTGCTGATTTTCTAGGTCGGTATAAGAAAATTTGGAAGAGCTCACTCTCAAATTTTACACAAGTGGAAGCTGCATGTTGTGGAGCTGGGAAGCTCGGTGGTGAATCCTTCTGTACACCAGATGCTAATCTTTGTTCGAACCGCGACCAATACTTGTTCTGGGATCTATTCCATCCAACACAGGCGGCTTCTAAGTTGGCAGCTGTGACCCTCTACTACGGAGGACCACAATTTGTATCCCCAATTAACTTCGCTCAGTTACCCGAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

169

Amino Acids

18.35

Weight (kDa)

8.38

Isoelectric Point (pI)

24.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 381
AciI CCGC 2 cut(s) 379, 422
AclWI GGATC 1 cut(s) 408
AcsI RAATTY 2 cut(s) 267, 289
AfaI GTAC 2 cut(s) 81, 352
AfiI CCNNNNNNNGG 2 cut(s) 179, 216
AgsI TTSAA 1 cut(s) 114
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 6 cut(s) 71, 279, 308, 323, 331, 440
AluI AGCT 6 cut(s) 71, 279, 308, 323, 331, 440
Alw21I GWGCWC 1 cut(s) 281
AlwI GGATC 1 cut(s) 408
Ama87I CYCGRG 1 cut(s) 500
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 5 cut(s) 62, 65, 68, 308, 437
ApoI RAATTY 2 cut(s) 267, 289
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 3 cut(s) 119, 192, 350
AsuII TTCGAA 1 cut(s) 374
AvaI CYCGRG 1 cut(s) 500
AvaII GGWCC 1 cut(s) 461
BanII GRGCYC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 5 cut(s) 74, 77, 80, 295, 449
BccI CCATC 1 cut(s) 417
BciT130I CCWGG 1 cut(s) 147
BciVI GTATCC 1 cut(s) 484
BfaI CTAG 3 cut(s) 26, 104, 251
BfuI GTATCC 1 cut(s) 484
BisI GCNGC 6 cut(s) 63, 66, 69, 309, 423, 438
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 6 cut(s) 64, 67, 70, 310, 424, 439
BmcAI AGTACT 1 cut(s) 81
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 1 cut(s) 461
BmeT110I CYCGRG 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 349
Bpu1102I GCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 374
BsaBI GATNNNNATC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 501
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 216
Bse1I ACTGG 2 cut(s) 77, 217
Bse3DI GCAATG 1 cut(s) 232
Bse8I GATNNNNATC 1 cut(s) 363
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 1 cut(s) 409
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 2 cut(s) 179, 216
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 506
BseNI ACTGG 2 cut(s) 77, 217
BseXI GCAGC 5 cut(s) 74, 77, 80, 295, 449
BseYI CCCAGC 1 cut(s) 323
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 281
BsiHKCI CYCGRG 1 cut(s) 500
BslI CCNNNNNNNGG 2 cut(s) 179, 216
BsnI GGCC 1 cut(s) 231
BsoBI CYCGRG 1 cut(s) 500
Bsp119I TTCGAA 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 281
Bsp1407I TGTACA 1 cut(s) 350
Bsp143I GATC 1 cut(s) 400
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 2 cut(s) 379, 422
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 1 cut(s) 408
BspQI GCTCTTC 1 cut(s) 269
BspT104I TTCGAA 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 232
BsrGI TGTACA 1 cut(s) 350
BsrI ACTGG 2 cut(s) 77, 217
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 1 cut(s) 400
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 239
Bst6I CTCTTC 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 350
BstBI TTCGAA 1 cut(s) 374
BstDEI CTNAG 4 cut(s) 53, 72, 429, 492
BstF5I GGATG 1 cut(s) 409
BstFNI CGCG 1 cut(s) 381
BstKTI GATC 1 cut(s) 403
BstMBI GATC 1 cut(s) 400
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 145
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 5 cut(s) 74, 77, 80, 295, 449
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuI GTATCC 1 cut(s) 484
BsuRI GGCC 1 cut(s) 231
BtsCI GGATG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 210, 244
Cfr13I GGNCC 1 cut(s) 461
Csp6I GTAC 2 cut(s) 80, 351
CviAII CATG 1 cut(s) 312
CviQI GTAC 2 cut(s) 80, 351
DdeI CTNAG 4 cut(s) 53, 72, 429, 492
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Ecl136II GAGCTC 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 461
Eco53kI GAGCTC 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 500
EcoICRI GAGCTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 281
FaeI CATG 1 cut(s) 315
FaiI YATR 4 cut(s) 44, 101, 261, 313
FatI CATG 1 cut(s) 311
Fnu4HI GCNGC 6 cut(s) 63, 66, 69, 309, 423, 438
FokI GGATG 1 cut(s) 396
FriOI GRGCYC 1 cut(s) 281
Fsp4HI GCNGC 6 cut(s) 63, 66, 69, 309, 423, 438
FspBI CTAG 3 cut(s) 26, 104, 251
GluI GCNGC 6 cut(s) 63, 66, 69, 309, 423, 438
GsaI CCCAGC 1 cut(s) 327
HaeIII GGCC 1 cut(s) 231
Hin1II CATG 1 cut(s) 315
HinfI GANTC 2 cut(s) 110, 341
HphI GGTGA 3 cut(s) 119, 192, 350
Hpy166II GTNNAC 1 cut(s) 353
Hpy8I GTNNAC 1 cut(s) 353
HpyAV CCTTC 2 cut(s) 210, 355
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4V TGCA 1 cut(s) 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 4 cut(s) 53, 72, 429, 492
Hsp92II CATG 1 cut(s) 315
Kzo9I GATC 1 cut(s) 400
LguI GCTCTTC 1 cut(s) 269
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 8 cut(s) 90, 132, 159, 198, 309, 369, 382, 404
Lsp1109I GCAGC 5 cut(s) 74, 77, 80, 295, 449
LweI GCATC 1 cut(s) 349
MaeI CTAG 3 cut(s) 26, 104, 251
MaeIII GTNAC 2 cut(s) 442, 495
MalI GATC 1 cut(s) 402
MboI GATC 1 cut(s) 400
MboII GAAGA 2 cut(s) 11, 286
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 1 cut(s) 281
MluCI AATT 5 cut(s) 93, 267, 289, 467, 480
MmeI TCCRAC 1 cut(s) 437
MnlI CCTC 5 cut(s) 33, 242, 452, 458, 496
MseI TTAA 2 cut(s) 483, 508
MspA1I CMGCKG 1 cut(s) 440
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 1 cut(s) 400
NlaIII CATG 1 cut(s) 315
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 442
NspI RCATGY 1 cut(s) 315
NspV TTCGAA 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 269
PfeI GAWTC 2 cut(s) 110, 341
PkrI GCNGC 6 cut(s) 64, 67, 70, 310, 424, 439
Psp124BI GAGCTC 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 461
PsuI RGATCY 1 cut(s) 400
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 2 cut(s) 81, 352
RsaNI GTAC 2 cut(s) 80, 351
SacI GAGCTC 1 cut(s) 281
SapI GCTCTTC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 483, 508
SatI GCNGC 6 cut(s) 63, 66, 69, 309, 423, 438
Sau3AI GATC 1 cut(s) 400
Sau96I GGNCC 1 cut(s) 461
ScaI AGTACT 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 281
SfaNI GCATC 1 cut(s) 349
SfuI TTCGAA 1 cut(s) 374
SinI GGWCC 1 cut(s) 461
Sse9I AATT 5 cut(s) 93, 267, 289, 467, 480
SsiI CCGC 2 cut(s) 379, 422
SspMI CTAG 3 cut(s) 26, 104, 251
SstI GAGCTC 1 cut(s) 281
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 1 cut(s) 239
TaqI TCGA 1 cut(s) 374
TasI AATT 5 cut(s) 93, 267, 289, 467, 480
TatI WGTACW 2 cut(s) 79, 350
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 2 cut(s) 110, 341
Tru1I TTAA 2 cut(s) 483, 508
Tru9I TTAA 2 cut(s) 483, 508
TscAI CASTG 2 cut(s) 217, 244
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 5 cut(s) 62, 65, 68, 308, 437
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 88
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 2 cut(s) 217, 244
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 2 cut(s) 267, 289
XceI RCATGY 1 cut(s) 315
XspI CTAG 3 cut(s) 26, 104, 251
ZrmI AGTACT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.