Rh2CG121400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000448
Physical Location & Seq
Forward (+)
10778754 .. 10780972
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG121400.1

Sequence Viewer

Length: 444 bp
ATGGCAAAGTCGAAGCTGATGATGCTTTTTGTTTCATTTTGTTTGTTATCTGCGTTTAGTGCATCTGAGGTAGCTTCTCCTGCGGCCATTTTTATATTTGGAGACTCAACTGTGGATGTTGGCACCAATACCTTGTTGCTCCAAAGTGGAGCCAACGCTGATTTCTCTCCAAATGGGGTTGATTTTCCTTATTCTGTGCCTGCTGGGAGGTTCAGCAATGGCCTTAACAGCGCTGATCAAATCGGTACAACCATCCTTAAGAAAGCTGCACAATATCATCAATGGCCTGGTTCAGCAGCACATCAAGGATCAGCCCCTCAACTTCCACACAGATCTCTTCGATTTCTTCTCGAAGAGCTCCTCGACCTTACACCCTCGTGGATCAGTAGGGTTGTGTCACCGCCGTCGTCGACGATCAGATCTGGGCCTCCGCCGGGACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

147

Amino Acids

15.53

Weight (kDa)

6.82

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 410
AciI CCGC 3 cut(s) 83, 401, 431
AclWI GGATC 2 cut(s) 316, 389
AcoI YGGCCR 1 cut(s) 84
AfaI GTAC 1 cut(s) 247
AfeI AGCGCT 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 434
AflII CTTAAG 1 cut(s) 257
AjnI CCWGG 1 cut(s) 286
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AleI CACNNNNGTG 1 cut(s) 376
AluBI AGCT 4 cut(s) 16, 74, 266, 358
AluI AGCT 4 cut(s) 16, 74, 266, 358
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 2 cut(s) 316, 389
Aor51HI AGCGCT 1 cut(s) 232
AoxI GGCC 4 cut(s) 84, 220, 284, 425
ApeKI GCWGC 2 cut(s) 266, 296
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 425, 437
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 1 cut(s) 390
AvaII GGWCC 1 cut(s) 437
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 1 cut(s) 376
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 2 cut(s) 253, 308
BccI CCATC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 1 cut(s) 288
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 1 cut(s) 442
BfoI RGCGCY 1 cut(s) 234
BfrI CTTAAG 1 cut(s) 257
BglII AGATCT 2 cut(s) 332, 419
BisI GCNGC 3 cut(s) 84, 267, 297
BlsI GCNGC 3 cut(s) 85, 268, 298
Bme1390I CCNGG 2 cut(s) 288, 435
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 425, 437
BmiI GGNNCC 4 cut(s) 124, 151, 438, 439
BmrFI CCNGG 2 cut(s) 288, 435
BmsI GCATC 2 cut(s) 12, 71
BpuMI CCSGG 1 cut(s) 435
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 1 cut(s) 288
BseGI GGATG 2 cut(s) 121, 252
BseLI CCNNNNNNNGG 1 cut(s) 434
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 2 cut(s) 253, 308
BseYI CCCAGC 1 cut(s) 203
BsgI GTGCAG 1 cut(s) 252
BshFI GGCC 4 cut(s) 86, 222, 286, 427
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 360
BsiSI CCGG 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 434
BsmAI GTCTC 1 cut(s) 96
BsnI GGCC 4 cut(s) 86, 222, 286, 427
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 6 cut(s) 235, 308, 332, 381, 414, 419
BspACI CCGC 3 cut(s) 83, 401, 431
BspANI GGCC 4 cut(s) 86, 222, 286, 427
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 4 cut(s) 124, 151, 438, 439
BspPI GGATC 2 cut(s) 316, 389
BspQI GCTCTTC 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 122
BspTI CTTAAG 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 223
BssMI GATC 6 cut(s) 235, 308, 332, 381, 414, 419
BssSI CACGAG 1 cut(s) 376
Bst2BI CACGAG 1 cut(s) 376
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 2 cut(s) 342, 348
BstAFI CTTAAG 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 121, 252
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 6 cut(s) 238, 311, 335, 384, 417, 422
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 6 cut(s) 235, 308, 332, 381, 414, 419
BstMWI GCNNNNNNNGC 4 cut(s) 22, 59, 80, 228
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 286, 433
BstV1I GCAGC 2 cut(s) 253, 308
BstX2I RGATCY 2 cut(s) 332, 419
BstYI RGATCY 2 cut(s) 332, 419
BsuRI GGCC 4 cut(s) 86, 222, 286, 427
BtsCI GGATG 2 cut(s) 121, 252
Cac8I GCNNGC 1 cut(s) 201
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 425, 437
Csp6I GTAC 1 cut(s) 246
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 1 cut(s) 66
DpnI GATC 6 cut(s) 237, 310, 334, 383, 416, 421
DpnII GATC 6 cut(s) 235, 308, 332, 381, 414, 419
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 342, 348
EarI CTCTTC 2 cut(s) 342, 348
EciI GGCGGA 1 cut(s) 420
Ecl136II GAGCTC 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 360
Eco47I GGWCC 1 cut(s) 437
Eco47III AGCGCT 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 360
FaiI YATR 1 cut(s) 95
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 1 cut(s) 410
Fnu4HI GCNGC 3 cut(s) 84, 267, 297
FokI GGATG 2 cut(s) 128, 239
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 3 cut(s) 84, 267, 297
FspBI CTAG 1 cut(s) 442
GlaI GCGC 1 cut(s) 232
GluI GCNGC 3 cut(s) 84, 267, 297
GsaI CCCAGC 1 cut(s) 207
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 4 cut(s) 86, 222, 286, 427
HapII CCGG 1 cut(s) 434
HhaI GCGC 1 cut(s) 233
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HinfI GANTC 1 cut(s) 104
HpaII CCGG 1 cut(s) 434
HphI GGTGA 1 cut(s) 390
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 2 cut(s) 67, 419
Hpy188III TCNNGA 1 cut(s) 350
Hpy8I GTNNAC 1 cut(s) 411
Hpy99I CGWCG 3 cut(s) 409, 412, 415
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 2 cut(s) 62, 269
HpyF10VI GCNNNNNNNGC 4 cut(s) 22, 59, 80, 228
HpyF3I CTNAG 1 cut(s) 66
HspAI GCGC 1 cut(s) 231
KflI GGGWCCC 1 cut(s) 437
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 6 cut(s) 235, 308, 332, 381, 414, 419
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 3 cut(s) 144, 149, 363
LpnPI CCDG 6 cut(s) 93, 189, 213, 273, 300, 408
Lsp1109I GCAGC 2 cut(s) 253, 308
LweI GCATC 2 cut(s) 12, 71
MaeI CTAG 1 cut(s) 442
MaeIII GTNAC 1 cut(s) 396
MalI GATC 6 cut(s) 237, 310, 334, 383, 416, 421
MboI GATC 6 cut(s) 235, 308, 332, 381, 414, 419
MboII GAAGA 3 cut(s) 329, 338, 365
MflI RGATCY 2 cut(s) 332, 419
MhlI GDGCHC 1 cut(s) 360
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 6 cut(s) 61, 201, 327, 371, 385, 438
MseI TTAA 2 cut(s) 225, 258
MslI CAYNNNNRTG 1 cut(s) 376
MspCI CTTAAG 1 cut(s) 257
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 2 cut(s) 288, 435
MvaI CCWGG 1 cut(s) 288
MwoI GCNNNNNNNGC 4 cut(s) 22, 59, 80, 228
NciI CCSGG 1 cut(s) 435
NdeII GATC 6 cut(s) 235, 308, 332, 381, 414, 419
NlaIV GGNNCC 4 cut(s) 124, 151, 438, 439
NmuCI GTSAC 1 cut(s) 396
OliI CACNNNNGTG 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 348
PkrI GCNGC 3 cut(s) 85, 268, 298
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 286
PspFI CCCAGC 1 cut(s) 203
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 4 cut(s) 124, 151, 438, 439
PspPI GGNCC 2 cut(s) 425, 437
PspPPI RGGWCCY 1 cut(s) 437
PsuI RGATCY 2 cut(s) 332, 419
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
RseI CAYNNNNRTG 1 cut(s) 376
SacI GAGCTC 1 cut(s) 360
SalI GTCGAC 1 cut(s) 409
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 2 cut(s) 225, 258
SatI GCNGC 3 cut(s) 84, 267, 297
Sau3AI GATC 6 cut(s) 235, 308, 332, 381, 414, 419
Sau96I GGNCC 2 cut(s) 425, 437
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 2 cut(s) 288, 435
SduI GDGCHC 1 cut(s) 360
SetI ASST 8 cut(s) 18, 72, 76, 134, 212, 268, 360, 369
SfaNI GCATC 2 cut(s) 12, 71
SgrDI CGTCGACG 1 cut(s) 409
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 376
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SsiI CCGC 3 cut(s) 83, 401, 431
SspMI CTAG 1 cut(s) 442
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 2 cut(s) 286, 433
TaaI ACNGT 1 cut(s) 112
TaqI TCGA 5 cut(s) 11, 340, 351, 363, 410
TauI GCSGC 1 cut(s) 86
Tru1I TTAA 2 cut(s) 225, 258
Tru9I TTAA 2 cut(s) 225, 258
TseFI GTSAC 1 cut(s) 396
TseI GCWGC 2 cut(s) 266, 296
Tsp45I GTSAC 1 cut(s) 396
TspDTI ATGAA 1 cut(s) 24
Vha464I CTTAAG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 410
XspI CTAG 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.