Rh6CG240400

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000448
Physical Location & Seq
Reverse (-)
40568755 .. 40570912
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG240400.1

Sequence Viewer

Length: 1074 bp
ATGGCAAACAAATTGGGTTTTCCTCTAGTTCTTCTATCCTTGGTCATAGCTGTTCTTAATTTAGCAGAAGCAGCTGTACCAGCAATCTTCATACTAGGTGACTCCACTGCTGATGTTGGCACCAACAACTTCTTGCCAGGTAGCATGGCCAGGGCTGATTTCCCCTACAATGGAATCGACTTTTGTAACTCAACACCCACTGGAAGGTTCAGCAATGGCCTTAATACAGCTGATTTTCTTGCCAAGCTATTGGGTTACGAGAAAAGTCCAAAGCCCTTTCTTTCCCTCAACTCTACTTCTCTTCTCAAGTGGTTTGGAGGCTTCAGCTTTGCTTCTGGAGGGTCTGGCCTCTTTGATACCACTGGACAAAGAATGACAAAAAATTGTATTCCACTAGCAGAGCAGATACAGCAATTTTCGTCTGTCCGGAGTAATCTCACCGCCTTAATGGGCCCGGTAGCAACTGAGACGTATCTTTCAAAGTCTTTGTTCTTCATCAGCATTGGCAGCAATGACATTTTCGAATACTACAGCTCAAACAGTTCAATTCCTGAGGAACAATTCTTGTCCTCTTTAGTACTCGCTTATGAGAATCACTTGAAGACGTTACTCAATCTTGGAGCAAGGAAGTTAGGAATTATCAGTGTTGCCCCGATTGGCTGCTGCCCATCTCAGAGATTTTTTAATGCTACTGGCGGCTGTATAGAGGAGCTGAATGATCATGCAAAAGCTTTCCATTCAAAGCTGGATGTGCTTATGCACAAGCTCAGCTCAGAATTTGAGGGCTTGAAGTACTCACTTGGAAATGCATTTGAAATGACTATAAATGTCATACAGAACCCTCTTGCATTCAATTTTACACAAGTGGCAGCTGCATGTTGTGGAACTGGGAAGCTCAATGCTGAAAACTTCTGTAAACCAGATGCAAATCTCTGTTCGAATCGCGATCAATACTTGTTCTGGGATCGATTTCATCCAACACAGGCTGCTTATAAGTTGGCTGCTGTAACCCTCTATGGTGGTGGACCACAATTTGTAACCCCAATTAATTTTGCTCAGTTGGCGGAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.65

Weight (kDa)

5.97

Isoelectric Point (pI)

21.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 335 1.6e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AccB1I GGYRCC 1 cut(s) 119
AccII CGCG 1 cut(s) 945
AccIII TCCGGA 1 cut(s) 426
AciI CCGC 3 cut(s) 441, 696, 1064
AclWI GGATC 1 cut(s) 972
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 1 cut(s) 776
AcuI CTGAAG 1 cut(s) 307
AfaI GTAC 3 cut(s) 78, 579, 794
AfiI CCNNNNNNNGG 2 cut(s) 170, 204
AgsI TTSAA 7 cut(s) 480, 546, 601, 741, 790, 815, 853
AjnI CCWGG 2 cut(s) 136, 149
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 1 cut(s) 972
Aor13HI TCCGGA 1 cut(s) 426
AoxI GGCC 4 cut(s) 147, 217, 346, 451
ApaI GGGCCC 1 cut(s) 455
ApeKI GCWGC 8 cut(s) 71, 507, 660, 663, 869, 872, 986, 1001
ApoI RAATTY 1 cut(s) 776
AseI ATTAAT 1 cut(s) 1047
AspS9I GGNCC 3 cut(s) 451, 452, 1025
AsuC2I CCSGG 1 cut(s) 455
AsuHPI GGTGA 2 cut(s) 110, 430
AsuII TTCGAA 2 cut(s) 522, 938
AvaII GGWCC 1 cut(s) 1025
AxyI CCTNAGG 1 cut(s) 552
BaeGI GKGCMC 1 cut(s) 455
BalI TGGCCA 1 cut(s) 149
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 455
BarI GAAGNNNNNNTAC 2 cut(s) 60, 92
BbsI GAAGAC 1 cut(s) 608
BbvI GCAGC 8 cut(s) 83, 519, 647, 650, 859, 881, 973, 988
BccI CCATC 1 cut(s) 676
BciT130I CCWGG 2 cut(s) 138, 151
BclI TGATCA 1 cut(s) 718
BcnI CCSGG 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 3 cut(s) 26, 95, 395
BfmI CTRYAG 1 cut(s) 529
BisI GCNGC 9 cut(s) 72, 508, 661, 664, 697, 870, 873, 987, 1002
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 9 cut(s) 73, 509, 662, 665, 698, 871, 874, 988, 1003
BmcAI AGTACT 2 cut(s) 579, 794
Bme1390I CCNGG 3 cut(s) 138, 151, 455
Bme18I GGWCC 1 cut(s) 1025
BmgT120I GGNCC 3 cut(s) 451, 452, 1025
BmiI GGNNCC 2 cut(s) 121, 453
BmrFI CCNGG 3 cut(s) 138, 151, 455
BmrI ACTGGG 1 cut(s) 897
BmsI GCATC 1 cut(s) 913
BmuI ACTGGG 1 cut(s) 897
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 2 cut(s) 522, 938
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 455
Bsa29I ATCGAT 1 cut(s) 967
BsaBI GATNNNNATC 1 cut(s) 927
BsaJI CCNNGG 2 cut(s) 39, 150
BsaWI WCCGGW 1 cut(s) 426
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 204
Bse1I ACTGG 4 cut(s) 205, 367, 697, 892
Bse21I CCTNAGG 1 cut(s) 552
Bse3DI GCAATG 2 cut(s) 220, 517
Bse8I GATNNNNATC 1 cut(s) 927
BseAI TCCGGA 1 cut(s) 426
BseBI CCWGG 2 cut(s) 138, 151
BseCI ATCGAT 1 cut(s) 967
BseDI CCNNGG 2 cut(s) 39, 150
BseGI GGATG 2 cut(s) 754, 973
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 2 cut(s) 170, 204
BseMI GCAATG 2 cut(s) 220, 517
BseMII CTCAG 6 cut(s) 456, 543, 686, 781, 786, 1070
BseNI ACTGG 4 cut(s) 205, 367, 697, 892
BseRI GAGGAG 1 cut(s) 722
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 8 cut(s) 83, 519, 647, 650, 859, 881, 973, 988
Bsh1236I CGCG 1 cut(s) 945
BshFI GGCC 4 cut(s) 149, 219, 348, 453
BshNI GGYRCC 1 cut(s) 119
BshVI ATCGAT 1 cut(s) 967
BsiSI CCGG 2 cut(s) 427, 455
BslI CCNNNNNNNGG 2 cut(s) 170, 204
BsmAI GTCTC 1 cut(s) 461
BsmBI CGTCTC 1 cut(s) 461
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 4 cut(s) 149, 219, 348, 453
Bsp119I TTCGAA 2 cut(s) 522, 938
Bsp120I GGGCCC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 426
Bsp143I GATC 3 cut(s) 718, 946, 964
Bsp1720I GCTNAGC 1 cut(s) 767
Bsp68I TCGCGA 1 cut(s) 945
BspACI CCGC 3 cut(s) 441, 696, 1064
BspANI GGCC 4 cut(s) 149, 219, 348, 453
BspCNI CTCAG 6 cut(s) 457, 544, 685, 780, 785, 1069
BspDI ATCGAT 1 cut(s) 967
BspEI TCCGGA 1 cut(s) 426
BspFNI CGCG 1 cut(s) 945
BspLI GGNNCC 2 cut(s) 121, 453
BspPI GGATC 1 cut(s) 972
BspT104I TTCGAA 2 cut(s) 522, 938
BspT107I GGYRCC 1 cut(s) 119
BsrDI GCAATG 2 cut(s) 220, 517
BsrI ACTGG 4 cut(s) 205, 367, 697, 892
BssECI CCNNGG 2 cut(s) 39, 150
BssMI GATC 3 cut(s) 718, 946, 964
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 2 cut(s) 138, 151
Bst4CI ACNGT 1 cut(s) 542
Bst6I CTCTTC 1 cut(s) 306
BstBI TTCGAA 2 cut(s) 522, 938
BstDEI CTNAG 6 cut(s) 465, 552, 672, 767, 772, 1056
BstF5I GGATG 2 cut(s) 754, 973
BstFNI CGCG 1 cut(s) 945
BstKTI GATC 3 cut(s) 721, 949, 967
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 3 cut(s) 718, 946, 964
BstMWI GCNNNNNNNGC 6 cut(s) 71, 80, 409, 507, 751, 1061
BstNI CCWGG 2 cut(s) 138, 151
BstNSI RCATGY 1 cut(s) 879
BstSCI CCNGG 3 cut(s) 136, 149, 453
BstSFI CTRYAG 1 cut(s) 529
BstSLI GKGCMC 1 cut(s) 455
BstUI CGCG 1 cut(s) 945
BstV1I GCAGC 8 cut(s) 83, 519, 647, 650, 859, 881, 973, 988
BstV2I GAAGAC 1 cut(s) 608
BstXI CCANNNNNNTGG 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 967
Bsu36I CCTNAGG 1 cut(s) 552
BsuRI GGCC 4 cut(s) 149, 219, 348, 453
BsuTUI ATCGAT 1 cut(s) 967
BtsCI GGATG 2 cut(s) 754, 973
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 4 cut(s) 105, 198, 360, 649
BtuMI TCGCGA 1 cut(s) 945
Cfr13I GGNCC 3 cut(s) 451, 452, 1025
ClaI ATCGAT 1 cut(s) 967
Csp6I GTAC 3 cut(s) 77, 578, 793
CviAII CATG 3 cut(s) 145, 722, 876
CviQI GTAC 3 cut(s) 77, 578, 793
DdeI CTNAG 6 cut(s) 465, 552, 672, 767, 772, 1056
DpnI GATC 3 cut(s) 720, 948, 966
DpnII GATC 3 cut(s) 718, 946, 964
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 1025
Eco57I CTGAAG 1 cut(s) 307
Eco81I CCTNAGG 1 cut(s) 552
EcoRII CCWGG 2 cut(s) 136, 149
EcoT14I CCWWGG 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 811
EcoT38I GRGCYC 1 cut(s) 455
ErhI CCWWGG 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 461
FaeI CATG 3 cut(s) 148, 725, 879
FatI CATG 3 cut(s) 144, 721, 875
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 9 cut(s) 72, 508, 661, 664, 697, 870, 873, 987, 1002
FokI GGATG 2 cut(s) 761, 960
FriOI GRGCYC 1 cut(s) 455
Fsp4HI GCNGC 9 cut(s) 72, 508, 661, 664, 697, 870, 873, 987, 1002
FspBI CTAG 3 cut(s) 26, 95, 395
GluI GCNGC 9 cut(s) 72, 508, 661, 664, 697, 870, 873, 987, 1002
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 4 cut(s) 149, 219, 348, 453
HapII CCGG 2 cut(s) 427, 455
Hin1II CATG 3 cut(s) 148, 725, 879
HindIII AAGCTT 1 cut(s) 729
HinfI GANTC 4 cut(s) 101, 174, 592, 940
HpaII CCGG 2 cut(s) 427, 455
HphI GGTGA 2 cut(s) 110, 430
Hpy166II GTNNAC 2 cut(s) 917, 1025
Hpy188I TCNGA 2 cut(s) 675, 775
Hpy188III TCNNGA 4 cut(s) 336, 427, 551, 944
Hpy8I GTNNAC 2 cut(s) 917, 1025
HpyAV CCTTC 1 cut(s) 198
HpyCH4III ACNGT 1 cut(s) 542
HpyCH4IV ACGT 2 cut(s) 470, 605
HpyCH4V TGCA 6 cut(s) 725, 760, 809, 848, 875, 926
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 80, 409, 507, 751, 1061
HpyF3I CTNAG 6 cut(s) 465, 552, 672, 767, 772, 1056
HpySE526I ACGT 2 cut(s) 470, 605
Hsp92II CATG 3 cut(s) 148, 725, 879
Kpn2I TCCGGA 1 cut(s) 426
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 3 cut(s) 718, 946, 964
LmnI GCTCC 2 cut(s) 620, 709
Lsp1109I GCAGC 8 cut(s) 83, 519, 647, 650, 859, 881, 973, 988
LweI GCATC 1 cut(s) 913
MaeI CTAG 3 cut(s) 26, 95, 395
MaeII ACGT 2 cut(s) 470, 605
MaeIII GTNAC 6 cut(s) 98, 185, 254, 606, 1006, 1036
MalI GATC 3 cut(s) 720, 948, 966
MboI GATC 3 cut(s) 718, 946, 964
MboII GAAGA 5 cut(s) 23, 79, 293, 484, 613
MhlI GDGCHC 1 cut(s) 455
MlsI TGGCCA 1 cut(s) 149
MluNI TGGCCA 1 cut(s) 149
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 1001
Mox20I TGGCCA 1 cut(s) 149
Mph1103I ATGCAT 1 cut(s) 811
MroI TCCGGA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 149
MseI TTAA 6 cut(s) 57, 222, 446, 684, 1047, 1072
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 3 cut(s) 74, 230, 872
MspI CCGG 2 cut(s) 427, 455
MspR9I CCNGG 3 cut(s) 138, 151, 455
Mva1269I GAATGC 1 cut(s) 848
MvaI CCWGG 2 cut(s) 138, 151
MvnI CGCG 1 cut(s) 945
MwoI GCNNNNNNNGC 6 cut(s) 71, 80, 409, 507, 751, 1061
NciI CCSGG 1 cut(s) 455
NdeII GATC 3 cut(s) 718, 946, 964
NlaIII CATG 3 cut(s) 148, 725, 879
NlaIV GGNNCC 2 cut(s) 121, 453
NmuCI GTSAC 1 cut(s) 98
NruI TCGCGA 1 cut(s) 945
NsiI ATGCAT 1 cut(s) 811
NspI RCATGY 1 cut(s) 879
NspV TTCGAA 2 cut(s) 522, 938
PctI GAATGC 1 cut(s) 848
PfeI GAWTC 3 cut(s) 174, 592, 940
PkrI GCNGC 9 cut(s) 73, 509, 662, 665, 698, 871, 874, 988, 1003
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PshBI ATTAAT 1 cut(s) 1047
PsiI TTATAA 1 cut(s) 993
Psp6I CCWGG 2 cut(s) 136, 149
PspGI CCWGG 2 cut(s) 136, 149
PspN4I GGNNCC 2 cut(s) 121, 453
PspOMI GGGCCC 1 cut(s) 451
PspPI GGNCC 3 cut(s) 451, 452, 1025
PvuII CAGCTG 3 cut(s) 74, 230, 872
RruI TCGCGA 1 cut(s) 945
RsaI GTAC 3 cut(s) 78, 579, 794
RsaNI GTAC 3 cut(s) 77, 578, 793
SaqAI TTAA 6 cut(s) 57, 222, 446, 684, 1047, 1072
SatI GCNGC 9 cut(s) 72, 508, 661, 664, 697, 870, 873, 987, 1002
Sau3AI GATC 3 cut(s) 718, 946, 964
Sau96I GGNCC 3 cut(s) 451, 452, 1025
ScaI AGTACT 2 cut(s) 579, 794
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 3 cut(s) 138, 151, 455
SduI GDGCHC 1 cut(s) 455
SfaNI GCATC 1 cut(s) 913
SfcI CTRYAG 1 cut(s) 529
SfuI TTCGAA 2 cut(s) 522, 938
SinI GGWCC 1 cut(s) 1025
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SsiI CCGC 3 cut(s) 441, 696, 1064
SspMI CTAG 3 cut(s) 26, 95, 395
StyD4I CCNGG 3 cut(s) 136, 149, 453
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 542
TaiI ACGT 2 cut(s) 473, 608
TaqI TCGA 4 cut(s) 177, 522, 938, 967
TatI WGTACW 2 cut(s) 577, 792
TauI GCSGC 1 cut(s) 699
TfiI GAWTC 3 cut(s) 174, 592, 940
Tru1I TTAA 6 cut(s) 57, 222, 446, 684, 1047, 1072
Tru9I TTAA 6 cut(s) 57, 222, 446, 684, 1047, 1072
TscAI CASTG 4 cut(s) 112, 205, 367, 649
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 8 cut(s) 71, 507, 660, 663, 869, 872, 986, 1001
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 3 cut(s) 79, 484, 962
TspRI CASTG 4 cut(s) 112, 205, 367, 649
VpaK11BI GGWCC 1 cut(s) 1025
VspI ATTAAT 1 cut(s) 1047
XapI RAATTY 1 cut(s) 776
XceI RCATGY 1 cut(s) 879
XspI CTAG 3 cut(s) 26, 95, 395
ZrmI AGTACT 2 cut(s) 579, 794
Zsp2I ATGCAT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.