pycom05g12470

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000448
Physical Location & Seq
Reverse (-)
15907285 .. 15908325
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12470.1

Sequence Viewer

Length: 636 bp
ATGAAGTTTGGAACACCAGAGAAAACTCCGCTTGCTGCCCCATCCATCAATCAAAAAAATGTTGTGTCATTAACAGAGCAGATACAACAATTTGCTACCGTCAAGAGAAATCTTACCGCCATAAAAGGGAGGAGGAAAGTAACTAAGAAGTCTTTGTTCTTCATCAGCATTGGTAGCAATGACCTTTTCGAATATTACCACTCAAACAGTTCCATTCCAAAGGAAGAATTCTTAACTTCTTTATTACTTGCTTATGAAAACCACTTGAAGGATTTGAACGATCTTGCACTAGCTTTCCATGGAAGGTTGGATGCCTTAATGTTCAAGCTGAGCTCAGAATGTAAGGACATCAAGTATGCACTTGGGAATGCATTTGAGATGACAATAAATGTCATTCAGAATCCTCTTCCATTTAATTTTACACAAGTGGAAGCTGCATGTTGTGGAGCTGGGAAGCTCAACGGTGAATCCTTCTGCAGCCCATATGCCAATCTTTCTTTGAATCGCGACAATTACTTGTTCTGGGATCCATATCATCCAACACAGGCTGCTTCTAAGTTAGCAGCTGTGACCCTCTTCAATGGTGGACCACAATTCGTAACCCCAATAAATTTTGCTCAGTTGGCCATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

212

Amino Acids

23.48

Weight (kDa)

8.61

Isoelectric Point (pI)

25.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 24 - 189 4e-10 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 507
AciI CCGC 2 cut(s) 29, 117
AclWI GGATC 2 cut(s) 521, 534
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 2 cut(s) 227, 610
AfiI CCNNNNNNNGG 2 cut(s) 126, 268
AgsI TTSAA 5 cut(s) 268, 277, 325, 502, 580
AluBI AGCT 7 cut(s) 293, 328, 333, 434, 449, 457, 566
AluI AGCT 7 cut(s) 293, 328, 333, 434, 449, 457, 566
Alw21I GWGCWC 1 cut(s) 335
AlwI GGATC 2 cut(s) 521, 534
AoxI GGCC 1 cut(s) 624
ApeKI GCWGC 5 cut(s) 35, 434, 477, 548, 563
ApoI RAATTY 2 cut(s) 227, 610
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 476
AsuII TTCGAA 1 cut(s) 189
AvaII GGWCC 1 cut(s) 587
BalI TGGCCA 1 cut(s) 626
BamHI GGATCC 1 cut(s) 526
BanII GRGCYC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 335
BbvI GCAGC 5 cut(s) 22, 421, 489, 535, 575
BccI CCATC 2 cut(s) 49, 53
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 475
BisI GCNGC 5 cut(s) 36, 435, 478, 549, 564
BlpI GCTNAGC 1 cut(s) 329
BlsI GCNGC 5 cut(s) 37, 436, 479, 550, 565
Bme18I GGWCC 1 cut(s) 587
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 528
BmsI GCATC 1 cut(s) 301
Bpu1102I GCTNAGC 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 189
BsaBI GATNNNNATC 1 cut(s) 531
BsaJI CCNNGG 2 cut(s) 298, 627
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 268
Bse3DI GCAATG 1 cut(s) 184
Bse8I GATNNNNATC 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 298, 627
BseGI GGATG 3 cut(s) 41, 316, 535
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 2 cut(s) 126, 268
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 3 cut(s) 320, 348, 632
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 5 cut(s) 22, 421, 489, 535, 575
BseYI CCCAGC 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 507
BshFI GGCC 1 cut(s) 626
BsiHKAI GWGCWC 1 cut(s) 335
BslI CCNNNNNNNGG 2 cut(s) 126, 268
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 1 cut(s) 626
Bsp119I TTCGAA 1 cut(s) 189
Bsp1286I GDGCHC 1 cut(s) 335
Bsp143I GATC 2 cut(s) 280, 526
Bsp1720I GCTNAGC 1 cut(s) 329
Bsp19I CCATGG 2 cut(s) 298, 627
Bsp68I TCGCGA 1 cut(s) 507
BspACI CCGC 2 cut(s) 29, 117
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 3 cut(s) 321, 347, 631
BspFNI CGCG 1 cut(s) 507
BspLI GGNNCC 1 cut(s) 528
BspMAI CTGCAG 1 cut(s) 479
BspPI GGATC 2 cut(s) 521, 534
BspT104I TTCGAA 1 cut(s) 189
BsrDI GCAATG 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 298, 627
BssMI GATC 2 cut(s) 280, 526
BssT1I CCWWGG 2 cut(s) 298, 627
Bst4CI ACNGT 3 cut(s) 100, 209, 464
Bst6I CTCTTC 2 cut(s) 411, 581
BstBI TTCGAA 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 5 cut(s) 144, 329, 334, 555, 618
BstDSI CCRYGG 2 cut(s) 298, 627
BstF5I GGATG 3 cut(s) 41, 316, 535
BstFNI CGCG 1 cut(s) 507
BstKTI GATC 2 cut(s) 283, 529
BstMBI GATC 2 cut(s) 280, 526
BstMWI GCNNNNNNNGC 2 cut(s) 174, 623
BstNSI RCATGY 1 cut(s) 441
BstSFI CTRYAG 1 cut(s) 475
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 5 cut(s) 22, 421, 489, 535, 575
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 1 cut(s) 626
BtgI CCRYGG 2 cut(s) 298, 627
BtsCI GGATG 3 cut(s) 41, 316, 535
BtuMI TCGCGA 1 cut(s) 507
Cac8I GCNNGC 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 587
CviAII CATG 3 cut(s) 299, 438, 628
DdeI CTNAG 5 cut(s) 144, 329, 334, 555, 618
DpnI GATC 2 cut(s) 282, 528
DpnII GATC 2 cut(s) 280, 526
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 2 cut(s) 411, 581
EarI CTCTTC 2 cut(s) 411, 581
Ecl136II GAGCTC 1 cut(s) 333
Eco130I CCWWGG 2 cut(s) 298, 627
Eco24I GRGCYC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 587
Eco53kI GAGCTC 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 227
EcoT14I CCWWGG 2 cut(s) 298, 627
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 335
ErhI CCWWGG 2 cut(s) 298, 627
FaeI CATG 3 cut(s) 302, 441, 631
FaiI YATR 9 cut(s) 122, 255, 300, 357, 439, 484, 486, 532, 629
FatI CATG 3 cut(s) 298, 437, 627
FauNDI CATATG 1 cut(s) 484
Fnu4HI GCNGC 5 cut(s) 36, 435, 478, 549, 564
FokI GGATG 3 cut(s) 28, 323, 522
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 5 cut(s) 36, 435, 478, 549, 564
FspBI CTAG 1 cut(s) 290
GluI GCNGC 5 cut(s) 36, 435, 478, 549, 564
GsaI CCCAGC 1 cut(s) 453
HaeIII GGCC 1 cut(s) 626
Hin1II CATG 3 cut(s) 302, 441, 631
HinfI GANTC 3 cut(s) 400, 467, 502
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 1 cut(s) 587
Hpy188I TCNGA 2 cut(s) 337, 399
Hpy188III TCNNGA 2 cut(s) 103, 506
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 3 cut(s) 262, 297, 481
HpyCH4III ACNGT 3 cut(s) 100, 209, 464
HpyCH4V TGCA 5 cut(s) 287, 359, 371, 437, 477
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 623
HpyF3I CTNAG 5 cut(s) 144, 329, 334, 555, 618
Hsp92II CATG 3 cut(s) 302, 441, 631
Kzo9I GATC 2 cut(s) 280, 526
LmnI GCTCC 1 cut(s) 446
LpnPI CCDG 4 cut(s) 30, 435, 508, 530
Lsp1109I GCAGC 5 cut(s) 22, 421, 489, 535, 575
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 290
MaeIII GTNAC 3 cut(s) 139, 568, 598
MalI GATC 2 cut(s) 282, 528
MboI GATC 2 cut(s) 280, 526
MboII GAAGA 4 cut(s) 151, 236, 398, 568
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 1 cut(s) 335
MlsI TGGCCA 1 cut(s) 626
MluCI AATT 6 cut(s) 89, 227, 415, 511, 593, 610
MluNI TGGCCA 1 cut(s) 626
MmeI TCCRAC 2 cut(s) 288, 563
MnlI CCTC 4 cut(s) 123, 126, 414, 584
Mox20I TGGCCA 1 cut(s) 626
Mph1103I ATGCAT 1 cut(s) 373
MscI TGGCCA 1 cut(s) 626
MseI TTAA 5 cut(s) 71, 233, 317, 414, 634
Msp20I TGGCCA 1 cut(s) 626
MspA1I CMGCKG 1 cut(s) 566
Mva1269I GAATGC 1 cut(s) 373
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 2 cut(s) 174, 623
NcoI CCATGG 2 cut(s) 298, 627
NdeI CATATG 1 cut(s) 484
NdeII GATC 2 cut(s) 280, 526
NlaIII CATG 3 cut(s) 302, 441, 631
NlaIV GGNNCC 1 cut(s) 528
NmuCI GTSAC 1 cut(s) 568
NruI TCGCGA 1 cut(s) 507
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 1 cut(s) 441
NspV TTCGAA 1 cut(s) 189
PctI GAATGC 1 cut(s) 373
PfeI GAWTC 3 cut(s) 400, 467, 502
PkrI GCNGC 5 cut(s) 37, 436, 479, 550, 565
Psp124BI GAGCTC 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 449
PspN4I GGNNCC 1 cut(s) 528
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 566
RruI TCGCGA 1 cut(s) 507
SacI GAGCTC 1 cut(s) 335
SaqAI TTAA 5 cut(s) 71, 233, 317, 414, 634
SatI GCNGC 5 cut(s) 36, 435, 478, 549, 564
Sau3AI GATC 2 cut(s) 280, 526
Sau96I GGNCC 1 cut(s) 587
SduI GDGCHC 1 cut(s) 335
SetI ASST 9 cut(s) 186, 295, 308, 330, 335, 436, 451, 459, 568
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 475
SfuI TTCGAA 1 cut(s) 189
SinI GGWCC 1 cut(s) 587
Sse9I AATT 6 cut(s) 89, 227, 415, 511, 593, 610
SsiI CCGC 2 cut(s) 29, 117
SspI AATATT 1 cut(s) 194
SspMI CTAG 1 cut(s) 290
SstI GAGCTC 1 cut(s) 335
StyI CCWWGG 2 cut(s) 298, 627
TaaI ACNGT 3 cut(s) 100, 209, 464
TaqI TCGA 1 cut(s) 189
TasI AATT 6 cut(s) 89, 227, 415, 511, 593, 610
TfiI GAWTC 3 cut(s) 400, 467, 502
Tru1I TTAA 5 cut(s) 71, 233, 317, 414, 634
Tru9I TTAA 5 cut(s) 71, 233, 317, 414, 634
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 5 cut(s) 35, 434, 477, 548, 563
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 3 cut(s) 17, 151, 270
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 2 cut(s) 227, 610
XceI RCATGY 1 cut(s) 441
XspI CTAG 1 cut(s) 290
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.