pycom05g12460

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000448
Physical Location & Seq
Reverse (-)
15903817 .. 15905531
1715 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12460.1

Sequence Viewer

Length: 1185 bp
ATGGAAAACATATCATGGGCTGTTGTTTCTCTAGTTTTATGTGTGGCTATAGGTTTTGTTAGTCCAGCAGACCCAGGTGCGCCACTAATCTTCATATTTGGTGACTCAACTGCTGATGTTGGAACAAACAATTTCTTGCCCACCAGCATGGCTAGGGCTGACTTTCCCCACAATGGCATTGACTTTCCTTCCTCAGAACCCACTGGAAGGTTCAGCAATGGTCTCAACAGTGCTGATTTTCTTTCTATGACATTCGGTCTCAAGAGAAGTCCGCCGCCATTTTTCTCTCTCAACGCTACGTATCTTTCCAACAAGACATTTGGTGGTGTTAACTTTGCTTCTGGAGGGTCAGGCCTATTTGACATAACTGGAAAAAAATCAGTGGTACGCCTGGCTCTTATGAAGTATGGAATGCCAAAGAAAACTCAGTTTGATGCCTCATCCGTCAGTCAGAAAAATGTTGTCCCATTAGCAGAGCAGATACAACAATTTTCCACTGTCAAGAGCAATCTTACCGCCATAAAAGGGGGGAGGAGAGTAACTGAGAGGTCCTTGTTCTTCATCAGCGTTGGTAGCAATGACCTTTTCGGATATTACCACTCAAACAGTTCCATACCAAAGGAAGAATTCTTATCTTCTTTATTACTTGCTTATGAAAACCACTTGAAGGGCTTAATCGATCTTGGAGCGAGGAAGTTTGGCATCATTAGCGTTGCCCCAATCGGATGCTGTCCATCTCAAAGGGTTCACAACGCCTCTGGCGGGTGTTTGGAAGATTTAAATGATCTTGCTGTAACTTTCCACTCAAGGTTGGATGCCCTAATGTCCAAGCTGAGCTCAGAATACAAGAACGTCAAGTATGCACTTGGCAATGCATTTGAGATGACAATAAATGTCATTCGGAACCCTCTTCCGTTCAGTAAGAACAATCTAATAACCAACAAATCTCCTTTTCATTTTTCAGATTTTACACAAGTGGAAGCTGCGTGTTGCGGAGCTGGGAAGCTCAACGGTGAATCCTTCTGCAGTCCAAATGCCACTCTTTGTTTGAATCGTGACAACTACTTGTTCTGGGATCTATTTCATCCAACACAGGCTGCTTCTAAGTTAGCAGCTGTTACCCTTTTCAATGGTGGACCACAATTCGTAACCCCGATAAATTTCGCTCAGTTGGCCATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

395

Amino Acids

42.58

Weight (kDa)

8.55

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 372 7.1e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 272, 275, 516, 762, 993
AclWI GGATC 1 cut(s) 1083
AcoI YGGCCR 1 cut(s) 1173
AcsI RAATTY 2 cut(s) 626, 1159
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 5 cut(s) 173, 207, 525, 667, 762
AgsI TTSAA 3 cut(s) 667, 1051, 1129
AhdI GACNNNNNGTC 1 cut(s) 255
AjnI CCWGG 2 cut(s) 73, 390
AluBI AGCT 6 cut(s) 832, 837, 983, 998, 1006, 1115
AluI AGCT 6 cut(s) 832, 837, 983, 998, 1006, 1115
Alw21I GWGCWC 1 cut(s) 839
Alw26I GTCTC 2 cut(s) 227, 263
AlwI GGATC 1 cut(s) 1083
AoxI GGCC 2 cut(s) 352, 1173
ApeKI GCWGC 3 cut(s) 983, 1097, 1112
ApoI RAATTY 2 cut(s) 626, 1159
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 2 cut(s) 549, 1136
AsuHPI GGTGA 2 cut(s) 113, 1025
AvaII GGWCC 2 cut(s) 549, 1136
BalI TGGCCA 1 cut(s) 1175
BanII GRGCYC 1 cut(s) 839
Bbv12I GWGCWC 1 cut(s) 839
BbvI GCAGC 3 cut(s) 970, 1084, 1124
BccI CCATC 1 cut(s) 742
BciT130I CCWGG 2 cut(s) 75, 392
BcoDI GTCTC 2 cut(s) 227, 263
BfaI CTAG 2 cut(s) 32, 153
BfmI CTRYAG 2 cut(s) 48, 1024
BisI GCNGC 4 cut(s) 275, 984, 1098, 1113
BlpI GCTNAGC 1 cut(s) 833
BlsI GCNGC 4 cut(s) 276, 985, 1099, 1114
Bme1390I CCNGG 2 cut(s) 75, 392
Bme18I GGWCC 2 cut(s) 549, 1136
BmeRI GACNNNNNGTC 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 549, 1136
BmiI GGNNCC 1 cut(s) 905
BmrFI CCNGG 2 cut(s) 75, 392
BmsI GCATC 4 cut(s) 424, 711, 716, 805
BpmI CTGGAG 1 cut(s) 363
Bpu1102I GCTNAGC 1 cut(s) 833
BpuEI CTTGAG 2 cut(s) 245, 790
Bsa29I ATCGAT 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 300
BsaI GGTCTC 2 cut(s) 227, 263
BsaJI CCNNGG 2 cut(s) 73, 1176
Bsc4I CCNNNNNNNGG 5 cut(s) 173, 207, 525, 667, 762
Bse1I ACTGG 2 cut(s) 208, 373
Bse3DI GCAATG 3 cut(s) 223, 583, 877
BseBI CCWGG 2 cut(s) 75, 392
BseCI ATCGAT 1 cut(s) 678
BseDI CCNNGG 2 cut(s) 73, 1176
BseGI GGATG 4 cut(s) 440, 731, 820, 1084
BseLI CCNNNNNNNGG 5 cut(s) 173, 207, 525, 667, 762
BseMI GCAATG 3 cut(s) 223, 583, 877
BseMII CTCAG 6 cut(s) 207, 440, 534, 824, 852, 1181
BseNI ACTGG 2 cut(s) 208, 373
BseRI GAGGAG 1 cut(s) 547
BseXI GCAGC 3 cut(s) 970, 1084, 1124
BseYI CCCAGC 1 cut(s) 998
BshFI GGCC 2 cut(s) 354, 1175
BshVI ATCGAT 1 cut(s) 678
BsiHKAI GWGCWC 1 cut(s) 839
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 5 cut(s) 173, 207, 525, 667, 762
BsmAI GTCTC 2 cut(s) 227, 263
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 1 cut(s) 417
BsnI GGCC 2 cut(s) 354, 1175
Bso31I GGTCTC 2 cut(s) 227, 263
Bsp1286I GDGCHC 1 cut(s) 839
Bsp143I GATC 3 cut(s) 679, 784, 1075
Bsp1720I GCTNAGC 1 cut(s) 833
Bsp19I CCATGG 1 cut(s) 1176
BspACI CCGC 5 cut(s) 272, 275, 516, 762, 993
BspANI GGCC 2 cut(s) 354, 1175
BspCNI CTCAG 6 cut(s) 206, 439, 535, 825, 851, 1180
BspDI ATCGAT 1 cut(s) 678
BspLI GGNNCC 1 cut(s) 905
BspMAI CTGCAG 1 cut(s) 1028
BspPI GGATC 1 cut(s) 1083
BspTNI GGTCTC 2 cut(s) 227, 263
BsrDI GCAATG 3 cut(s) 223, 583, 877
BsrI ACTGG 2 cut(s) 208, 373
BssECI CCNNGG 2 cut(s) 73, 1176
BssMI GATC 3 cut(s) 679, 784, 1075
BssT1I CCWWGG 1 cut(s) 1176
Bst2UI CCWGG 2 cut(s) 75, 392
Bst4CI ACNGT 4 cut(s) 230, 499, 608, 1013
Bst6I CTCTTC 1 cut(s) 915
BstBAI YACGTR 1 cut(s) 300
BstDEI CTNAG 7 cut(s) 193, 426, 543, 833, 838, 1104, 1167
BstDSI CCRYGG 1 cut(s) 1176
BstF5I GGATG 4 cut(s) 440, 731, 820, 1084
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 3 cut(s) 682, 787, 1078
BstMAI GTCTC 2 cut(s) 227, 263
BstMBI GATC 3 cut(s) 679, 784, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 573, 708, 1172
BstNI CCWGG 2 cut(s) 75, 392
BstSCI CCNGG 2 cut(s) 73, 390
BstSFI CTRYAG 2 cut(s) 48, 1024
BstSNI TACGTA 1 cut(s) 300
BstV1I GCAGC 3 cut(s) 970, 1084, 1124
BstX2I RGATCY 1 cut(s) 1075
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 1 cut(s) 1075
Bsu15I ATCGAT 1 cut(s) 678
BsuRI GGCC 2 cut(s) 354, 1175
BsuTUI ATCGAT 1 cut(s) 678
BtgI CCRYGG 1 cut(s) 1176
BtsCI GGATG 4 cut(s) 440, 731, 820, 1084
BtsIMutI CAGTG 4 cut(s) 201, 235, 387, 495
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 549, 1136
ClaI ATCGAT 1 cut(s) 678
Csp6I GTAC 1 cut(s) 386
CviAII CATG 3 cut(s) 15, 148, 1177
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 7 cut(s) 193, 426, 543, 833, 838, 1104, 1167
DpnI GATC 3 cut(s) 681, 786, 1077
DpnII GATC 3 cut(s) 679, 784, 1075
DraI TTTAAA 1 cut(s) 780
DriI GACNNNNNGTC 1 cut(s) 255
EaeI YGGCCR 1 cut(s) 1173
Eam1104I CTCTTC 1 cut(s) 915
Eam1105I GACNNNNNGTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 915
EciI GGCGGA 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 837
Eco105I TACGTA 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 1176
Eco147I AGGCCT 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 839
Eco31I GGTCTC 2 cut(s) 227, 263
Eco47I GGWCC 2 cut(s) 549, 1136
Eco53kI GAGCTC 1 cut(s) 837
EcoICRI GAGCTC 1 cut(s) 837
EcoO109I RGGNCCY 1 cut(s) 549
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 2 cut(s) 73, 390
EcoT14I CCWWGG 1 cut(s) 1176
EcoT22I ATGCAT 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 839
ErhI CCWWGG 1 cut(s) 1176
FaeI CATG 3 cut(s) 18, 151, 1180
FaqI GGGAC 1 cut(s) 449
FatI CATG 3 cut(s) 14, 147, 1176
FauI CCCGC 1 cut(s) 755
Fnu4HI GCNGC 4 cut(s) 275, 984, 1098, 1113
FokI GGATG 4 cut(s) 427, 738, 827, 1071
FriOI GRGCYC 1 cut(s) 839
Fsp4HI GCNGC 4 cut(s) 275, 984, 1098, 1113
FspBI CTAG 2 cut(s) 32, 153
GlaI GCGC 1 cut(s) 81
GluI GCNGC 4 cut(s) 275, 984, 1098, 1113
GsaI CCCAGC 1 cut(s) 1002
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 2 cut(s) 354, 1175
HhaI GCGC 1 cut(s) 82
Hin1II CATG 3 cut(s) 18, 151, 1180
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 3 cut(s) 104, 1016, 1051
HpaI GTTAAC 1 cut(s) 331
HphI GGTGA 2 cut(s) 113, 1025
Hpy166II GTNNAC 3 cut(s) 331, 748, 1136
Hpy188I TCNGA 7 cut(s) 196, 453, 590, 725, 841, 903, 964
Hpy188III TCNNGA 4 cut(s) 262, 342, 502, 1055
Hpy8I GTNNAC 3 cut(s) 331, 748, 1136
HpyAV CCTTC 4 cut(s) 198, 201, 661, 1030
HpyCH4III ACNGT 4 cut(s) 230, 499, 608, 1013
HpyCH4IV ACGT 2 cut(s) 299, 852
HpyCH4V TGCA 3 cut(s) 863, 875, 1026
HpyF10VI GCNNNNNNNGC 3 cut(s) 573, 708, 1172
HpyF3I CTNAG 7 cut(s) 193, 426, 543, 833, 838, 1104, 1167
HpySE526I ACGT 2 cut(s) 299, 852
Hsp92II CATG 3 cut(s) 18, 151, 1180
HspAI GCGC 1 cut(s) 80
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 679, 784, 1075
LmnI GCTCC 2 cut(s) 686, 995
Lsp1109I GCAGC 3 cut(s) 970, 1084, 1124
LweI GCATC 4 cut(s) 424, 711, 716, 805
MaeI CTAG 2 cut(s) 32, 153
MaeII ACGT 2 cut(s) 299, 852
MaeIII GTNAC 6 cut(s) 101, 538, 793, 1055, 1117, 1147
MalI GATC 3 cut(s) 681, 786, 1077
MboI GATC 3 cut(s) 679, 784, 1075
MboII GAAGA 6 cut(s) 82, 550, 627, 635, 785, 902
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 1 cut(s) 839
MlsI TGGCCA 1 cut(s) 1175
MluCI AATT 5 cut(s) 130, 488, 626, 1142, 1159
MluNI TGGCCA 1 cut(s) 1175
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 4 cut(s) 100, 333, 792, 1112
MnlI CCTC 8 cut(s) 202, 338, 448, 525, 540, 684, 766, 918
Mox20I TGGCCA 1 cut(s) 1175
Mph1103I ATGCAT 1 cut(s) 877
MscI TGGCCA 1 cut(s) 1175
MseI TTAA 4 cut(s) 330, 674, 779, 1183
MslI CAYNNNNRTG 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 1175
MspA1I CMGCKG 1 cut(s) 1115
MspR9I CCNGG 2 cut(s) 75, 392
Mva1269I GAATGC 1 cut(s) 417
MvaI CCWGG 2 cut(s) 75, 392
MwoI GCNNNNNNNGC 3 cut(s) 573, 708, 1172
NcoI CCATGG 1 cut(s) 1176
NdeII GATC 3 cut(s) 679, 784, 1075
NlaIII CATG 3 cut(s) 18, 151, 1180
NlaIV GGNNCC 1 cut(s) 905
NmuCI GTSAC 2 cut(s) 101, 1055
NsiI ATGCAT 1 cut(s) 877
PceI AGGCCT 1 cut(s) 354
PctI GAATGC 1 cut(s) 417
PfeI GAWTC 2 cut(s) 1016, 1051
PkrI GCNGC 4 cut(s) 276, 985, 1099, 1114
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Ppu21I YACGTR 1 cut(s) 300
PpuMI RGGWCCY 1 cut(s) 549
Psp124BI GAGCTC 1 cut(s) 839
Psp5II RGGWCCY 1 cut(s) 549
Psp6I CCWGG 2 cut(s) 73, 390
PspFI CCCAGC 1 cut(s) 998
PspGI CCWGG 2 cut(s) 73, 390
PspN4I GGNNCC 1 cut(s) 905
PspPI GGNCC 2 cut(s) 549, 1136
PspPPI RGGWCCY 1 cut(s) 549
PstI CTGCAG 1 cut(s) 1028
PsuI RGATCY 1 cut(s) 1075
PvuII CAGCTG 1 cut(s) 1115
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
RseI CAYNNNNRTG 1 cut(s) 146
SacI GAGCTC 1 cut(s) 839
SaqAI TTAA 4 cut(s) 330, 674, 779, 1183
SatI GCNGC 4 cut(s) 275, 984, 1098, 1113
Sau3AI GATC 3 cut(s) 679, 784, 1075
Sau96I GGNCC 2 cut(s) 549, 1136
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 2 cut(s) 75, 392
SduI GDGCHC 1 cut(s) 839
SfaNI GCATC 4 cut(s) 424, 711, 716, 805
SfcI CTRYAG 2 cut(s) 48, 1024
SinI GGWCC 2 cut(s) 549, 1136
SmiI ATTTAAAT 1 cut(s) 780
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 2 cut(s) 260, 805
SmoI CTYRAG 2 cut(s) 260, 805
SnaBI TACGTA 1 cut(s) 300
Sse9I AATT 5 cut(s) 130, 488, 626, 1142, 1159
SseBI AGGCCT 1 cut(s) 354
SsiI CCGC 5 cut(s) 272, 275, 516, 762, 993
SspMI CTAG 2 cut(s) 32, 153
SstI GAGCTC 1 cut(s) 839
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 2 cut(s) 73, 390
StyI CCWWGG 1 cut(s) 1176
SwaI ATTTAAAT 1 cut(s) 780
TaaI ACNGT 4 cut(s) 230, 499, 608, 1013
TaiI ACGT 2 cut(s) 302, 855
TaqI TCGA 1 cut(s) 678
TaqII GACCGA 1 cut(s) 245
TasI AATT 5 cut(s) 130, 488, 626, 1142, 1159
TauI GCSGC 1 cut(s) 277
TfiI GAWTC 2 cut(s) 1016, 1051
Tru1I TTAA 4 cut(s) 330, 674, 779, 1183
Tru9I TTAA 4 cut(s) 330, 674, 779, 1183
TscAI CASTG 4 cut(s) 208, 235, 387, 502
TseFI GTSAC 2 cut(s) 101, 1055
TseI GCWGC 3 cut(s) 983, 1097, 1112
Tsp45I GTSAC 2 cut(s) 101, 1055
TspDTI ATGAA 6 cut(s) 82, 416, 550, 669, 944, 1073
TspGWI ACGGA 2 cut(s) 433, 903
TspRI CASTG 4 cut(s) 208, 235, 387, 502
VpaK11BI GGWCC 2 cut(s) 549, 1136
XapI RAATTY 2 cut(s) 626, 1159
XspI CTAG 2 cut(s) 32, 153
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.