Rw6G020430

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000448
Physical Location & Seq
Reverse (-)
40746864 .. 40748570
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G020430.1

Sequence Viewer

Length: 1107 bp
ATGGAAAACAAATGGGTTCTTCCTCTAGCCCTTTCCTTGTTTATAACTGTTCTTAGCTTGGCAGCAGCAGCTAAGCCAGTACTGCCACCAGTCTTCATATTTGGTGATTCAACAGCAGATGTCGGCACCAATAACTTCTTGCCGGCTAGCAAGGCAAGAGCTAACTTTCCCCCCAATGGCATTGACTTTCCTGGCACCTCAAAACCCACCGGAAGGTTCAGCAATGGCCTTAACAGTGCTGATTTTCTTGTCCGGCATTTTGGTTACAAGAGAAGTCCAAGCCCCTTTCTTTCTCTGAAAACTACTTCTCTTCAAAAGAAGAAGTTTAATGGTATCAACTTTGCTTCTGGAGGGTCTGGTCTTCTTGACATAACTGGACGGACAATGGCAAGTCACTTTCTTAGGGAACAAAAAAATGTTATCTCATTAACAGAGCAGATACAGCAATTTTCATCTGTCAAGAACAATCTTACGGCCTTGATGGGTGCGGTAGCAACTGAGAAGTTTCTTAAAGATTCTTTGATCTTCATCAGCACCGGCAGCAATGACCTTTTCGGAAACTACCATTCAAAGAGTTCCATTCCAAAGGAAAAGTTCTTGTCCTCTTTAGAAGTAGCTTATGAGAATCACTTGAAGACTCTATACAATCTTGGAGCAAGGAAGTTTGGCATCATCAGCATTGCCCCGATTGGCTGCTGCCCATCTCAGAGGATTTTCAATGCTACTGGAGGATGTTTGGAAGAGCTGAATGATAATGCAATAGCTTTTCATTCAAGATTGGATGCCCTCTTGTGCAAGCTTAGCTCAGAATACAAGGGCATGAAGTACTCACTTGGAAATTCATATGAAATGACAATCAATGTCATACAGAACCCTCTTCCATTCAATTTTACACAAGTGGCAGCTGCATGTTGTGGAACTGGGAAGCTCAATGCTGAAAACTATTGTAAACCACATGCAAATCTCTGTTCGGATCGCCATCAATATTTGTTCTGGGATCGATTTCATCCAACACAGGCTGCTTCTAAGTTGGCCGCTATTGCCCTCTACAGTGGTGGACCACAATTTGTATCCCCAATTAATTTTTCTCAGTTGGCCAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

368

Amino Acids

40.11

Weight (kDa)

9.35

Isoelectric Point (pI)

26.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 347 6.7e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 44
AccB1I GGYRCC 2 cut(s) 125, 194
AciI CCGC 2 cut(s) 488, 1035
AclWI GGATC 2 cut(s) 981, 1005
AcoI YGGCCR 2 cut(s) 1032, 1095
AcsI RAATTY 1 cut(s) 838
AfaI GTAC 2 cut(s) 81, 827
AfiI CCNNNNNNNGG 2 cut(s) 176, 213
AgsI TTSAA 7 cut(s) 111, 314, 570, 634, 718, 774, 886
AjnI CCWGG 1 cut(s) 190
AjuI GAANNNNNNNTTGG 2 cut(s) 1069, 1101
AlwI GGATC 2 cut(s) 981, 1005
AoxI GGCC 4 cut(s) 226, 474, 1032, 1095
ApeKI GCWGC 9 cut(s) 62, 65, 68, 540, 693, 696, 902, 905, 1019
ApoI RAATTY 1 cut(s) 838
AseI ATTAAT 1 cut(s) 1080
AspS9I GGNCC 1 cut(s) 1058
AsuHPI GGTGA 1 cut(s) 116
AsuNHI GCTAGC 1 cut(s) 146
AvaII GGWCC 1 cut(s) 1058
BalI TGGCCA 1 cut(s) 1097
BanI GGYRCC 2 cut(s) 125, 194
BarI GAAGNNNNNNTAC 2 cut(s) 626, 658
BbsI GAAGAC 3 cut(s) 85, 353, 641
BbvI GCAGC 9 cut(s) 74, 77, 80, 552, 680, 683, 892, 914, 1006
BccI CCATC 3 cut(s) 475, 709, 987
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 1 cut(s) 192
BciVI GTATCC 1 cut(s) 1081
BfaI CTAG 2 cut(s) 26, 147
BfmI CTRYAG 1 cut(s) 1048
BfuI GTATCC 1 cut(s) 1081
BlpI GCTNAGC 2 cut(s) 72, 800
BmcAI AGTACT 2 cut(s) 81, 827
Bme1390I CCNGG 1 cut(s) 192
Bme18I GGWCC 1 cut(s) 1058
BmgT120I GGNCC 1 cut(s) 1058
BmiI GGNNCC 2 cut(s) 127, 196
BmrFI CCNGG 1 cut(s) 192
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 2 cut(s) 678, 772
BmtI GCTAGC 1 cut(s) 150
BmuI ACTGGG 1 cut(s) 930
BpiI GAAGAC 3 cut(s) 85, 353, 641
BpmI CTGGAG 2 cut(s) 369, 747
Bpu1102I GCTNAGC 2 cut(s) 72, 800
Bsa29I ATCGAT 1 cut(s) 1000
BsaBI GATNNNNATC 2 cut(s) 527, 978
BsaJI CCNNGG 1 cut(s) 1098
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 213
Bse118I RCCGGY 2 cut(s) 142, 536
Bse1I ACTGG 5 cut(s) 77, 89, 379, 730, 925
Bse3DI GCAATG 3 cut(s) 229, 550, 678
Bse8I GATNNNNATC 2 cut(s) 527, 978
BseBI CCWGG 1 cut(s) 192
BseCI ATCGAT 1 cut(s) 1000
BseDI CCNNGG 1 cut(s) 1098
BseGI GGATG 3 cut(s) 737, 787, 1006
BseJI GATNNNNATC 2 cut(s) 527, 978
BseLI CCNNNNNNNGG 2 cut(s) 176, 213
BseMI GCAATG 3 cut(s) 229, 550, 678
BseMII CTCAG 4 cut(s) 489, 719, 819, 1103
BseNI ACTGG 5 cut(s) 77, 89, 379, 730, 925
BseXI GCAGC 9 cut(s) 74, 77, 80, 552, 680, 683, 892, 914, 1006
BshFI GGCC 4 cut(s) 228, 476, 1034, 1097
BshNI GGYRCC 2 cut(s) 125, 194
BshVI ATCGAT 1 cut(s) 1000
BsiSI CCGG 4 cut(s) 143, 210, 253, 537
BslI CCNNNNNNNGG 2 cut(s) 176, 213
BsnI GGCC 4 cut(s) 228, 476, 1034, 1097
Bsp143I GATC 3 cut(s) 522, 973, 997
Bsp1720I GCTNAGC 2 cut(s) 72, 800
BspACI CCGC 2 cut(s) 488, 1035
BspANI GGCC 4 cut(s) 228, 476, 1034, 1097
BspCNI CTCAG 4 cut(s) 490, 718, 818, 1102
BspDI ATCGAT 1 cut(s) 1000
BspLI GGNNCC 2 cut(s) 127, 196
BspOI GCTAGC 1 cut(s) 150
BspPI GGATC 2 cut(s) 981, 1005
BspQI GCTCTTC 1 cut(s) 735
BspT107I GGYRCC 2 cut(s) 125, 194
BsrDI GCAATG 3 cut(s) 229, 550, 678
BsrFI RCCGGY 2 cut(s) 142, 536
BsrI ACTGG 5 cut(s) 77, 89, 379, 730, 925
BssAI RCCGGY 2 cut(s) 142, 536
BssECI CCNNGG 1 cut(s) 1098
BssMI GATC 3 cut(s) 522, 973, 997
BssT1I CCWWGG 1 cut(s) 1098
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 3 cut(s) 49, 236, 1052
Bst6I CTCTTC 3 cut(s) 315, 735, 882
BstC8I GCNNGC 3 cut(s) 144, 148, 797
BstF5I GGATG 3 cut(s) 737, 787, 1006
BstKTI GATC 3 cut(s) 525, 976, 1000
BstMBI GATC 3 cut(s) 522, 973, 997
BstMWI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 442, 540, 675, 801, 1040
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 2 cut(s) 912, 959
BstSCI CCNGG 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 1048
BstV1I GCAGC 9 cut(s) 74, 77, 80, 552, 680, 683, 892, 914, 1006
BstV2I GAAGAC 3 cut(s) 85, 353, 641
Bsu15I ATCGAT 1 cut(s) 1000
BsuI GTATCC 1 cut(s) 1081
BsuRI GGCC 4 cut(s) 228, 476, 1034, 1097
BsuTUI ATCGAT 1 cut(s) 1000
BtsCI GGATG 3 cut(s) 737, 787, 1006
BtsIMutI CAGTG 2 cut(s) 241, 1057
Cac8I GCNNGC 3 cut(s) 144, 148, 797
Cfr10I RCCGGY 2 cut(s) 142, 536
Cfr13I GGNCC 1 cut(s) 1058
ClaI ATCGAT 1 cut(s) 1000
Csp6I GTAC 2 cut(s) 80, 826
CviAII CATG 3 cut(s) 820, 909, 956
CviQI GTAC 2 cut(s) 80, 826
DpnI GATC 3 cut(s) 524, 975, 999
DpnII GATC 3 cut(s) 522, 973, 997
EaeI YGGCCR 2 cut(s) 1032, 1095
Eam1104I CTCTTC 3 cut(s) 315, 735, 882
EarI CTCTTC 3 cut(s) 315, 735, 882
Eco130I CCWWGG 1 cut(s) 1098
Eco47I GGWCC 1 cut(s) 1058
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 1098
ErhI CCWWGG 1 cut(s) 1098
FaeI CATG 3 cut(s) 823, 912, 959
FatI CATG 3 cut(s) 819, 908, 955
FauNDI CATATG 1 cut(s) 844
FokI GGATG 3 cut(s) 744, 794, 993
FspBI CTAG 2 cut(s) 26, 147
GsuI CTGGAG 2 cut(s) 369, 747
HaeIII GGCC 4 cut(s) 228, 476, 1034, 1097
HapII CCGG 4 cut(s) 143, 210, 253, 537
Hin1II CATG 3 cut(s) 823, 912, 959
HindIII AAGCTT 1 cut(s) 797
HinfI GANTC 4 cut(s) 107, 515, 625, 637
HpaII CCGG 4 cut(s) 143, 210, 253, 537
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 2 cut(s) 950, 1058
Hpy188I TCNGA 5 cut(s) 297, 557, 708, 808, 973
Hpy188III TCNNGA 4 cut(s) 348, 365, 460, 774
Hpy8I GTNNAC 2 cut(s) 950, 1058
HpyAV CCTTC 1 cut(s) 207
HpyCH4III ACNGT 3 cut(s) 49, 236, 1052
HpyCH4V TGCA 4 cut(s) 758, 795, 908, 959
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 442, 540, 675, 801, 1040
Hsp92II CATG 3 cut(s) 823, 912, 959
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Kzo9I GATC 3 cut(s) 522, 973, 997
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 1 cut(s) 653
Lsp1109I GCAGC 9 cut(s) 74, 77, 80, 552, 680, 683, 892, 914, 1006
LweI GCATC 2 cut(s) 678, 772
MaeI CTAG 2 cut(s) 26, 147
MaeIII GTNAC 2 cut(s) 263, 392
MalI GATC 3 cut(s) 524, 975, 999
MboI GATC 3 cut(s) 522, 973, 997
MboII GAAGA 9 cut(s) 11, 85, 302, 331, 353, 517, 646, 752, 869
MlsI TGGCCA 1 cut(s) 1097
MluCI AATT 6 cut(s) 446, 838, 886, 1064, 1077, 1081
MluNI TGGCCA 1 cut(s) 1097
MlyI GAGTC 1 cut(s) 631
MmeI TCCRAC 1 cut(s) 1034
MnlI CCTC 9 cut(s) 33, 208, 344, 613, 702, 722, 797, 885, 1055
Mox20I TGGCCA 1 cut(s) 1097
MroNI GCCGGC 1 cut(s) 142
MscI TGGCCA 1 cut(s) 1097
MseI TTAA 5 cut(s) 231, 327, 428, 510, 1080
Msp20I TGGCCA 1 cut(s) 1097
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 4 cut(s) 143, 210, 253, 537
MspR9I CCNGG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 442, 540, 675, 801, 1040
NaeI GCCGGC 1 cut(s) 144
NdeI CATATG 1 cut(s) 844
NdeII GATC 3 cut(s) 522, 973, 997
NgoMIV GCCGGC 1 cut(s) 142
NheI GCTAGC 1 cut(s) 146
NlaIII CATG 3 cut(s) 823, 912, 959
NlaIV GGNNCC 2 cut(s) 127, 196
NmuCI GTSAC 1 cut(s) 392
NspI RCATGY 2 cut(s) 912, 959
PciSI GCTCTTC 1 cut(s) 735
PdiI GCCGGC 1 cut(s) 144
PfeI GAWTC 3 cut(s) 107, 515, 625
PleI GAGTC 1 cut(s) 631
PpsI GAGTC 1 cut(s) 631
PshBI ATTAAT 1 cut(s) 1080
PsiI TTATAA 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 2 cut(s) 127, 196
PspPI GGNCC 1 cut(s) 1058
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 2 cut(s) 81, 827
RsaNI GTAC 2 cut(s) 80, 826
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 5 cut(s) 231, 327, 428, 510, 1080
Sau3AI GATC 3 cut(s) 522, 973, 997
Sau96I GGNCC 1 cut(s) 1058
ScaI AGTACT 2 cut(s) 81, 827
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 1 cut(s) 192
SfaNI GCATC 2 cut(s) 678, 772
SfcI CTRYAG 1 cut(s) 1048
SinI GGWCC 1 cut(s) 1058
Sse9I AATT 6 cut(s) 446, 838, 886, 1064, 1077, 1081
SsiI CCGC 2 cut(s) 488, 1035
SspI AATATT 1 cut(s) 986
SspMI CTAG 2 cut(s) 26, 147
StyD4I CCNGG 1 cut(s) 190
StyI CCWWGG 1 cut(s) 1098
TaaI ACNGT 3 cut(s) 49, 236, 1052
TaqI TCGA 1 cut(s) 1000
TasI AATT 6 cut(s) 446, 838, 886, 1064, 1077, 1081
TatI WGTACW 2 cut(s) 79, 825
TauI GCSGC 1 cut(s) 1037
TfiI GAWTC 3 cut(s) 107, 515, 625
Tru1I TTAA 5 cut(s) 231, 327, 428, 510, 1080
Tru9I TTAA 5 cut(s) 231, 327, 428, 510, 1080
TscAI CASTG 2 cut(s) 241, 1057
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 9 cut(s) 62, 65, 68, 540, 693, 696, 902, 905, 1019
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 8 cut(s) 85, 441, 517, 758, 831, 836, 861, 995
TspGWI ACGGA 1 cut(s) 394
TspRI CASTG 2 cut(s) 241, 1057
VpaK11BI GGWCC 1 cut(s) 1058
VspI ATTAAT 1 cut(s) 1080
XapI RAATTY 1 cut(s) 838
XceI RCATGY 2 cut(s) 912, 959
XspI CTAG 2 cut(s) 26, 147
ZrmI AGTACT 2 cut(s) 81, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.