MD15G1235500.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000448
Physical Location & Seq
Forward (+)
19360628 .. 19361637
1010 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1235500.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGAGGGACATCTCGTTGCATTTGGAGAACAGTTCGAGCAGTTTGCAAGAGTACACGACATTATATCAAAATTACAGGGTGAGAGTGGATTGGCTAATATATCAAAGTCTCTCTTCCTCTTCAGCGTTGGAGGCAATGACATCTTCGAACATTTTCTTTTTAACAATACTACTTCTGCAAAAGTCTTCATTGCGACTCTCATGTCCACTTATGAAAGTCGATTAAGGCGTCTTTTTGACCTTGGAGCCAAAAAATTTGGGATTATTAGTGTTCCACCAGTTGGGTGCGTCCCATTGCAGCGTAATTCTGCTAAAGATGGTAGCTGTTCTGAGCCCATGAATGAGTATGCTAAATTGTTTTACAAAGCACTTCAAGGAGTCTTGCAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

131

Amino Acids

14.44

Weight (kDa)

8.6

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 9 - 128 1.2e-10 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 202
AccB7I CCANNNNNTGG 1 cut(s) 281
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 106
AcyI GRCGYC 1 cut(s) 229
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 281
AgsI TTSAA 1 cut(s) 374
AluBI AGCT 1 cut(s) 324
AluI AGCT 1 cut(s) 324
Alw26I GTCTC 1 cut(s) 114
ApeKI GCWGC 1 cut(s) 298
ApoI RAATTY 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 92
AsuII TTCGAA 1 cut(s) 147
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 310
BccI CCATC 1 cut(s) 311
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BcoDI GTCTC 1 cut(s) 114
BisI GCNGC 1 cut(s) 299
BlsI GCNGC 1 cut(s) 300
BmiI GGNNCC 1 cut(s) 247
BpiI GAAGAC 1 cut(s) 178
Bpu14I TTCGAA 1 cut(s) 147
BsaHI GRCGYC 1 cut(s) 229
BsaJI CCNNGG 1 cut(s) 241
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 278
Bse3DI GCAATG 3 cut(s) 142, 189, 293
BseDI CCNNGG 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 3 cut(s) 142, 189, 293
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 1 cut(s) 278
BseXI GCAGC 1 cut(s) 310
BslFI GGGAC 2 cut(s) 21, 275
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 2 cut(s) 21, 275
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 322
BspLI GGNNCC 1 cut(s) 247
BspT104I TTCGAA 1 cut(s) 147
BsrDI GCAATG 3 cut(s) 142, 189, 293
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 1 cut(s) 241
BssNI GRCGYC 1 cut(s) 229
BssT1I CCWWGG 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 33
Bst6I CTCTTC 2 cut(s) 119, 125
BstACI GRCGYC 1 cut(s) 229
BstBI TTCGAA 1 cut(s) 147
BstDEI CTNAG 1 cut(s) 330
BstMAI GTCTC 1 cut(s) 114
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstV1I GCAGC 1 cut(s) 310
BstV2I GAAGAC 1 cut(s) 178
CseI GACGC 2 cut(s) 218, 277
Csp6I GTAC 1 cut(s) 53
CviAII CATG 2 cut(s) 202, 337
CviJI RGCY 4 cut(s) 95, 248, 324, 334
CviKI_1 RGCY 4 cut(s) 95, 248, 324, 334
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 1 cut(s) 330
DrdI GACNNNNNNGTC 1 cut(s) 202
DseDI GACNNNNNNGTC 1 cut(s) 202
Eam1104I CTCTTC 2 cut(s) 119, 125
EarI CTCTTC 2 cut(s) 119, 125
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 2 cut(s) 205, 340
FaiI YATR 6 cut(s) 65, 101, 203, 213, 338, 348
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 2 cut(s) 21, 275
FatI CATG 2 cut(s) 201, 336
Fnu4HI GCNGC 1 cut(s) 299
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 1 cut(s) 299
GluI GCNGC 1 cut(s) 299
HgaI GACGC 2 cut(s) 218, 277
Hin1I GRCGYC 1 cut(s) 229
Hin1II CATG 2 cut(s) 205, 340
HinfI GANTC 2 cut(s) 196, 378
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 2 cut(s) 55, 207
Hpy188I TCNGA 1 cut(s) 331
Hpy8I GTNNAC 2 cut(s) 55, 207
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 5 cut(s) 20, 47, 179, 298, 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 1 cut(s) 330
Hsp92I GRCGYC 1 cut(s) 229
Hsp92II CATG 2 cut(s) 205, 340
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 2 cut(s) 62, 291
Lsp1109I GCAGC 1 cut(s) 310
MboII GAAGA 4 cut(s) 106, 112, 136, 178
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 4 cut(s) 71, 254, 304, 353
MlyI GAGTC 2 cut(s) 190, 387
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 2 cut(s) 125, 128
MroXI GAANNNNTTC 1 cut(s) 153
MseI TTAA 2 cut(s) 162, 224
MwoI GCNNNNNNNGC 1 cut(s) 132
NlaIII CATG 2 cut(s) 205, 340
NlaIV GGNNCC 1 cut(s) 247
NspV TTCGAA 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 153
PflMI CCANNNNNTGG 1 cut(s) 281
PkrI GCNGC 1 cut(s) 300
PleI GAGTC 2 cut(s) 190, 386
PpsI GAGTC 2 cut(s) 190, 386
PspN4I GGNNCC 1 cut(s) 247
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
SaqAI TTAA 2 cut(s) 162, 224
SatI GCNGC 1 cut(s) 299
SchI GAGTC 2 cut(s) 190, 387
SduI GDGCHC 1 cut(s) 336
SetI ASST 2 cut(s) 243, 326
SfuI TTCGAA 1 cut(s) 147
Sse9I AATT 4 cut(s) 71, 254, 304, 353
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 3 cut(s) 36, 147, 220
TasI AATT 4 cut(s) 71, 254, 304, 353
TatI WGTACW 1 cut(s) 52
Tru1I TTAA 2 cut(s) 162, 224
Tru9I TTAA 2 cut(s) 162, 224
TseI GCWGC 1 cut(s) 298
TspDTI ATGAA 3 cut(s) 178, 228, 353
Van91I CCANNNNNTGG 1 cut(s) 281
XapI RAATTY 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.