MD05G1128700.v1.1

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000448
Physical Location & Seq
Reverse (-)
25247767 .. 25252862
5096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1128700.v1.1.491

Sequence Viewer

Length: 2301 bp
ATGGCAAACAAATCATGGGTTGTTGTTTCTCTGGTCCTCTGTGTGGCTGTAGCTGTTGTTAGTTCATCAGACCCAGGTGCGCCACTAATCTTCGTACTAGGTGACTCAACGGCCGACGTTGGAACCAATAACTTCTTGCCCACCAGCACGGCTAGGGCTAACTTTCCACACAATGGCATTGACTTTCCTTCCTCAAAACCCACTGGAAGGTTCAGCAATGGCCTCAATAGTGCTGATTTTCTCGCTCAGATACTCGGTCTCAGGAGAAGTCCGCCGCCTTTTCTTTCTCTCAAAGCTAAATCTCTTCGCAAGAAGAGGTTTAGTGGAGTTAACTTTGCTTCTGGAGGGTCAGGCCTTCTTGACATAACTGGAAAAACATCGGTGCGTTATTCAGAAAATTGTTATCTTCATAAGCAAGAGATGTTGGAAAAGAAACATTTTTTTCCAGTTTTCTCATTTTTCTTGTTTCAGCTGAGTCTAATGAAGTTTGGAACACCAGAGAAAACTCCGCTTGCTGCCCCATCCGTCAATCAAAAAAATGTTGTGTCATTAACAGAGCAGATTCAACAATTTGCTACCGTCAAGAGAAACCTTACCGCCATAAAAGGGGGGAGGAGAGTAACTAAGAAGTCCTTGTTCTTCATCAGCATTGGTAGCAATGACCTTTTCGAATATTACCACTCAAACAGTTCCATTCCAAAGGAAGAATTCTTAACTTCTTTATTACTTGCATATGAAAACCACTTGAAGAATTTAATTGATCTTGGAGCGAGGAAGTTTGGCATCATTAGCGTTGCCCCGATTGGATGCTGCCCGTCTCAAAGGATTCACAACACTACCAGCGGATGTTTGGAGGATTTGAACGATCTTGCAGTAGCTTTCCATGCAAGGTTGGATGCCTTAATGTTCAAGATGAGCTCAGAATGTAAGGACATCAAGTATGCACTTGGAAATGCTTTCGAGATGACAATAAATGTCATTCAGAATCCTCTTCCATATAATTTTACACAAGTGGAAGCTGCATGTTGCGGAGCTGGGAAGCTCAACGGTGAATCCTTCTGCAGTCCATATGCCAATCTTTGTTTGAATCGCGACAACTACTTGTTCTGGGATCTATATCATCCAACACAGGCTGCTTCTAAGTTAGCAGCTGTGACCCTCTTCAATGGTTTTGTTAGTCCAGCAGACCCAGGTGCGCCACTTATCTTCATATTTGGTGACTCAACTGCTGATGTTGGAACAAACAATTTCTTGCTCACCAGCACGGCTAGGGCTGACTTTCCCCACAATGGCATTGACTTTCCTTCCTCAGAACCCACTGGGAGGTTCAGCAATGGCCTCAACAGTGCTGATTTTCTTTCTATGACTTTCGGTCTCAAGAGAAGTCCGCCGCCATTTTTCTCTCTCAACGCTACGTCTCTTTCCAACAAGACATTTGGTGGTGTTAACTTTGCTTCTGGAGGGTCAGGCCTATTTGACATAACTGGAAAAAAATCAGTGCTGGCTCTGATGAAGTATGGAACGCCAAAGAAAACTCAGTTTGCTGCCTCATCCGTCAGTCAGAAAAATGTTGTCCCATTAGCAGAGCAGATACAACAATTTTCCACTGTCAAGAGCAATCTTACCGCCATAAAAGGGGGGAGGAGAGTAACTGAGAGGTCCTTGTTCTTTATCAGCATTGGTAGCAATGACCTTTTCGGATATTACCACTCAAACAGTTCCATACCAAAGGAAGAATTCTTAACTTCTTTATTACTTGCTTATGAAAACCACTTGAAGGGCTTAATCGATCTTGGAGCAAGGAAGTTTGGCATCATTAGCGTTGCCCCAATCGGATGCTGTCCATCTCAAAGGGTTTACAACGCCTCTGGCGGGTGTTTAGAGGATTTAAATGATCTTGCAGTAGCTTTCCACTCAAGGTTGGATGCCCTAATGTCCAAGCTAAGCTCAGAATGCAAGGACGTCAAGTATGCACTTGGCAATGCATTTGAGATGACAATAAATGTCATTCGGAACCCTCTTCCATTCAGTAAGTCAATACTCGACAGTTGTTATTGGAAAAAGACTTCCGCACACCCTTATTTTACACAAGTGGAAGCTGCCTGTTGCGGAGCTGGGAAGCTCAACGGTGAATCCTTCTGCAGTCCAAATGCCACACTTTGTTTGAATCGCAACAACTACTTGTTCTGGGATCTATTTCATCCAACACAGGCGGCCTCTAAGTTAGCAGCTGTGACCCTCTTCAATGGTGGACCACAATTCGTAACCCCGATAAATTTCGCTCAGTTGGCCATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

767

Amino Acids

83.26

Weight (kDa)

9.02

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 384 3.7e-29 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL PF00657 402 - 744 7.3e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1965
AccB7I CCANNNNNTGG 1 cut(s) 173
AccII CGCG 1 cut(s) 1092
AclWI GGATC 2 cut(s) 1119, 2199
AcoI YGGCCR 2 cut(s) 111, 2289
AcsI RAATTY 4 cut(s) 707, 751, 1736, 2275
AcyI GRCGYC 1 cut(s) 1962
AfaI GTAC 1 cut(s) 96
AfiI CCNNNNNNNGG 9 cut(s) 43, 173, 207, 606, 1289, 1323, 1635, 1777, 1872
AgsI TTSAA 9 cut(s) 566, 748, 862, 910, 1087, 1165, 1777, 2167, 2245
AhdI GACNNNNNGTC 1 cut(s) 1371
AjnI CCWGG 2 cut(s) 73, 1189
Alw21I GWGCWC 1 cut(s) 920
Alw26I GTCTC 4 cut(s) 263, 822, 1379, 1422
AlwI GGATC 2 cut(s) 1119, 2199
AoxI GGCC 7 cut(s) 111, 220, 352, 1336, 1468, 2214, 2289
ApeKI GCWGC 8 cut(s) 515, 810, 1019, 1133, 1148, 1544, 2099, 2228
ApoI RAATTY 4 cut(s) 707, 751, 1736, 2275
AspLEI GCGC 2 cut(s) 82, 1198
AspS9I GGNCC 3 cut(s) 34, 1659, 2252
AsuHPI GGTGA 5 cut(s) 113, 1061, 1229, 1249, 2141
AsuII TTCGAA 1 cut(s) 669
AvaII GGWCC 3 cut(s) 34, 1659, 2252
BalI TGGCCA 1 cut(s) 2291
BanII GRGCYC 1 cut(s) 920
Bbv12I GWGCWC 1 cut(s) 920
BbvI GCAGC 8 cut(s) 502, 797, 1006, 1120, 1160, 1531, 2086, 2240
BccI CCATC 2 cut(s) 529, 1852
BceAI ACGGC 3 cut(s) 126, 165, 1281
BciT130I CCWGG 2 cut(s) 75, 1191
BcoDI GTCTC 4 cut(s) 263, 822, 1379, 1422
BfaI CTAG 3 cut(s) 98, 153, 1269
BfmI CTRYAG 3 cut(s) 48, 1060, 2140
BlpI GCTNAGC 1 cut(s) 1943
Bme1390I CCNGG 2 cut(s) 75, 1191
Bme18I GGWCC 3 cut(s) 34, 1659, 2252
BmeRI GACNNNNNGTC 1 cut(s) 1371
BmgT120I GGNCC 3 cut(s) 34, 1659, 2252
BmiI GGNNCC 2 cut(s) 124, 2015
BmrFI CCNGG 2 cut(s) 75, 1191
BmrI ACTGGG 1 cut(s) 1329
BmsI GCATC 6 cut(s) 792, 797, 886, 1821, 1826, 1915
BmuI ACTGGG 1 cut(s) 1329
BpmI CTGGAG 2 cut(s) 363, 1479
Bpu1102I GCTNAGC 1 cut(s) 1943
Bpu14I TTCGAA 1 cut(s) 669
BpuEI CTTGAG 2 cut(s) 1361, 1900
Bsa29I ATCGAT 1 cut(s) 1788
BsaBI GATNNNNATC 1 cut(s) 1116
BsaHI GRCGYC 1 cut(s) 1962
BsaI GGTCTC 2 cut(s) 263, 1379
BsaJI CCNNGG 3 cut(s) 73, 1189, 2292
Bsc4I CCNNNNNNNGG 9 cut(s) 43, 173, 207, 606, 1289, 1323, 1635, 1777, 1872
Bse1I ACTGG 5 cut(s) 208, 373, 446, 1324, 1489
Bse3DI GCAATG 5 cut(s) 223, 664, 1339, 1693, 1987
Bse8I GATNNNNATC 1 cut(s) 1116
BseBI CCWGG 2 cut(s) 75, 1191
BseCI ATCGAT 1 cut(s) 1788
BseDI CCNNGG 3 cut(s) 73, 1189, 2292
BseGI GGATG 9 cut(s) 521, 812, 851, 901, 1120, 1550, 1841, 1930, 2200
BseJI GATNNNNATC 1 cut(s) 1116
BseLI CCNNNNNNNGG 9 cut(s) 43, 173, 207, 606, 1289, 1323, 1635, 1777, 1872
BseMI GCAATG 5 cut(s) 223, 664, 1339, 1693, 1987
BseMII CTCAG 9 cut(s) 260, 274, 464, 933, 1323, 1550, 1644, 1962, 2297
BseNI ACTGG 5 cut(s) 208, 373, 446, 1324, 1489
BseRI GAGGAG 2 cut(s) 628, 1657
BseX3I CGGCCG 1 cut(s) 111
BseXI GCAGC 8 cut(s) 502, 797, 1006, 1120, 1160, 1531, 2086, 2240
BseYI CCCAGC 2 cut(s) 1034, 2114
Bsh1236I CGCG 1 cut(s) 1092
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 7 cut(s) 113, 222, 354, 1338, 1470, 2216, 2291
BshVI ATCGAT 1 cut(s) 1788
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 920
BslFI GGGAC 1 cut(s) 1559
BslI CCNNNNNNNGG 9 cut(s) 43, 173, 207, 606, 1289, 1323, 1635, 1777, 1872
BsmAI GTCTC 4 cut(s) 263, 822, 1379, 1422
BsmBI CGTCTC 2 cut(s) 822, 1422
BsmFI GGGAC 1 cut(s) 1559
BsmI GAATGC 1 cut(s) 1958
BsnI GGCC 7 cut(s) 113, 222, 354, 1338, 1470, 2216, 2291
Bso31I GGTCTC 2 cut(s) 263, 1379
Bsp119I TTCGAA 1 cut(s) 669
Bsp1286I GDGCHC 1 cut(s) 920
Bsp143I GATC 6 cut(s) 760, 865, 1111, 1789, 1894, 2191
Bsp1720I GCTNAGC 1 cut(s) 1943
Bsp19I CCATGG 1 cut(s) 2292
Bsp68I TCGCGA 1 cut(s) 1092
BspANI GGCC 7 cut(s) 113, 222, 354, 1338, 1470, 2216, 2291
BspCNI CTCAG 9 cut(s) 259, 273, 465, 932, 1322, 1549, 1645, 1961, 2296
BspDI ATCGAT 1 cut(s) 1788
BspFNI CGCG 1 cut(s) 1092
BspLI GGNNCC 2 cut(s) 124, 2015
BspMAI CTGCAG 2 cut(s) 1064, 2144
BspPI GGATC 2 cut(s) 1119, 2199
BspT104I TTCGAA 1 cut(s) 669
BspTNI GGTCTC 2 cut(s) 263, 1379
BsrDI GCAATG 5 cut(s) 223, 664, 1339, 1693, 1987
BsrI ACTGG 5 cut(s) 208, 373, 446, 1324, 1489
BssECI CCNNGG 3 cut(s) 73, 1189, 2292
BssMI GATC 6 cut(s) 760, 865, 1111, 1789, 1894, 2191
BssNI GRCGYC 1 cut(s) 1962
BssT1I CCWWGG 1 cut(s) 2292
Bst2UI CCWGG 2 cut(s) 75, 1191
Bst4CI ACNGT 8 cut(s) 580, 689, 1049, 1346, 1609, 1718, 2048, 2129
Bst6I CTCTTC 6 cut(s) 308, 309, 996, 1166, 2025, 2246
BstACI GRCGYC 1 cut(s) 1962
BstBI TTCGAA 1 cut(s) 669
BstC8I GCNNGC 2 cut(s) 513, 1503
BstDSI CCRYGG 1 cut(s) 2292
BstF5I GGATG 9 cut(s) 521, 812, 851, 901, 1120, 1550, 1841, 1930, 2200
BstFNI CGCG 1 cut(s) 1092
BstHHI GCGC 2 cut(s) 82, 1198
BstKTI GATC 6 cut(s) 763, 868, 1114, 1792, 1897, 2194
BstMAI GTCTC 4 cut(s) 263, 822, 1379, 1422
BstMBI GATC 6 cut(s) 760, 865, 1111, 1789, 1894, 2191
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 7 cut(s) 654, 789, 884, 1683, 1818, 1953, 2288
BstNI CCWGG 2 cut(s) 75, 1191
BstNSI RCATGY 1 cut(s) 1026
BstSCI CCNGG 2 cut(s) 73, 1189
BstSFI CTRYAG 3 cut(s) 48, 1060, 2140
BstUI CGCG 1 cut(s) 1092
BstV1I GCAGC 8 cut(s) 502, 797, 1006, 1120, 1160, 1531, 2086, 2240
BstX2I RGATCY 2 cut(s) 1111, 2191
BstYI RGATCY 2 cut(s) 1111, 2191
BstZI CGGCCG 1 cut(s) 111
Bsu15I ATCGAT 1 cut(s) 1788
BsuRI GGCC 7 cut(s) 113, 222, 354, 1338, 1470, 2216, 2291
BsuTUI ATCGAT 1 cut(s) 1788
BtgI CCRYGG 1 cut(s) 2292
BtsCI GGATG 9 cut(s) 521, 812, 851, 901, 1120, 1550, 1841, 1930, 2200
BtsIMutI CAGTG 5 cut(s) 201, 1317, 1351, 1503, 1605
BtuMI TCGCGA 1 cut(s) 1092
Cac8I GCNNGC 2 cut(s) 513, 1503
CfoI GCGC 2 cut(s) 82, 1198
Cfr13I GGNCC 3 cut(s) 34, 1659, 2252
ClaI ATCGAT 1 cut(s) 1788
Csp6I GTAC 1 cut(s) 95
CviAII CATG 4 cut(s) 15, 884, 1023, 2293
CviQI GTAC 1 cut(s) 95
DpnI GATC 6 cut(s) 762, 867, 1113, 1791, 1896, 2193
DpnII GATC 6 cut(s) 760, 865, 1111, 1789, 1894, 2191
DraI TTTAAA 1 cut(s) 1890
DriI GACNNNNNGTC 1 cut(s) 1371
EaeI YGGCCR 2 cut(s) 111, 2289
EagI CGGCCG 1 cut(s) 111
Eam1104I CTCTTC 6 cut(s) 308, 309, 996, 1166, 2025, 2246
Eam1105I GACNNNNNGTC 1 cut(s) 1371
EarI CTCTTC 6 cut(s) 308, 309, 996, 1166, 2025, 2246
EciI GGCGGA 2 cut(s) 261, 1377
Ecl136II GAGCTC 1 cut(s) 918
EclXI CGGCCG 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 2292
Eco147I AGGCCT 2 cut(s) 354, 1470
Eco24I GRGCYC 1 cut(s) 920
Eco31I GGTCTC 2 cut(s) 263, 1379
Eco47I GGWCC 3 cut(s) 34, 1659, 2252
Eco52I CGGCCG 1 cut(s) 111
Eco53kI GAGCTC 1 cut(s) 918
EcoICRI GAGCTC 1 cut(s) 918
EcoO109I RGGNCCY 1 cut(s) 1659
EcoRI GAATTC 2 cut(s) 707, 1736
EcoRII CCWGG 2 cut(s) 73, 1189
EcoT14I CCWWGG 1 cut(s) 2292
EcoT22I ATGCAT 1 cut(s) 1987
EcoT38I GRGCYC 1 cut(s) 920
ErhI CCWWGG 1 cut(s) 2292
Esp3I CGTCTC 2 cut(s) 822, 1422
FaeI CATG 4 cut(s) 18, 887, 1026, 2296
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 1 cut(s) 1559
FatI CATG 4 cut(s) 14, 883, 1022, 2292
FauI CCCGC 1 cut(s) 1865
FauNDI CATATG 2 cut(s) 733, 1069
FokI GGATG 9 cut(s) 508, 819, 858, 908, 1107, 1537, 1848, 1937, 2187
FriOI GRGCYC 1 cut(s) 920
FspBI CTAG 3 cut(s) 98, 153, 1269
GlaI GCGC 2 cut(s) 81, 1197
GsaI CCCAGC 2 cut(s) 1038, 2118
GsuI CTGGAG 2 cut(s) 363, 1479
HaeIII GGCC 7 cut(s) 113, 222, 354, 1338, 1470, 2216, 2291
HhaI GCGC 2 cut(s) 82, 1198
Hin1I GRCGYC 1 cut(s) 1962
Hin1II CATG 4 cut(s) 18, 887, 1026, 2296
Hin6I GCGC 2 cut(s) 80, 1196
HinP1I GCGC 2 cut(s) 80, 1196
HincII GTYRAC 2 cut(s) 331, 1447
HindII GTYRAC 2 cut(s) 331, 1447
HpaI GTTAAC 2 cut(s) 331, 1447
HphI GGTGA 5 cut(s) 113, 1061, 1229, 1249, 2141
Hpy166II GTNNAC 4 cut(s) 331, 1447, 1858, 2252
Hpy8I GTNNAC 4 cut(s) 331, 1447, 1858, 2252
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 7 cut(s) 198, 201, 365, 1066, 1314, 1771, 2146
HpyCH4III ACNGT 8 cut(s) 580, 689, 1049, 1346, 1609, 1718, 2048, 2129
HpyCH4IV ACGT 3 cut(s) 117, 1415, 1962
HpyF10VI GCNNNNNNNGC 7 cut(s) 654, 789, 884, 1683, 1818, 1953, 2288
HpySE526I ACGT 3 cut(s) 117, 1415, 1962
Hsp92I GRCGYC 1 cut(s) 1962
Hsp92II CATG 4 cut(s) 18, 887, 1026, 2296
HspAI GCGC 2 cut(s) 80, 1196
KspAI GTTAAC 2 cut(s) 331, 1447
Kzo9I GATC 6 cut(s) 760, 865, 1111, 1789, 1894, 2191
LmnI GCTCC 4 cut(s) 767, 1031, 1796, 2111
Lsp1109I GCAGC 8 cut(s) 502, 797, 1006, 1120, 1160, 1531, 2086, 2240
LweI GCATC 6 cut(s) 792, 797, 886, 1821, 1826, 1915
MaeI CTAG 3 cut(s) 98, 153, 1269
MaeII ACGT 3 cut(s) 117, 1415, 1962
MaeIII GTNAC 7 cut(s) 101, 619, 1153, 1217, 1648, 2233, 2263
MalI GATC 6 cut(s) 762, 867, 1113, 1791, 1896, 2193
MboI GATC 6 cut(s) 760, 865, 1111, 1789, 1894, 2191
MflI RGATCY 2 cut(s) 1111, 2191
MhlI GDGCHC 1 cut(s) 920
MlsI TGGCCA 1 cut(s) 2291
MluNI TGGCCA 1 cut(s) 2291
MlyI GAGTC 3 cut(s) 98, 484, 1214
MmeI TCCRAC 8 cut(s) 100, 405, 873, 1148, 1216, 1449, 1902, 2228
Mox20I TGGCCA 1 cut(s) 2291
Mph1103I ATGCAT 1 cut(s) 1987
MscI TGGCCA 1 cut(s) 2291
Msp20I TGGCCA 1 cut(s) 2291
MspA1I CMGCKG 4 cut(s) 472, 843, 1151, 2231
MspR9I CCNGG 2 cut(s) 75, 1191
Mva1269I GAATGC 1 cut(s) 1958
MvaI CCWGG 2 cut(s) 75, 1191
MvnI CGCG 1 cut(s) 1092
MwoI GCNNNNNNNGC 7 cut(s) 654, 789, 884, 1683, 1818, 1953, 2288
NcoI CCATGG 1 cut(s) 2292
NdeI CATATG 2 cut(s) 733, 1069
NdeII GATC 6 cut(s) 760, 865, 1111, 1789, 1894, 2191
NlaIII CATG 4 cut(s) 18, 887, 1026, 2296
NlaIV GGNNCC 2 cut(s) 124, 2015
NmuCI GTSAC 4 cut(s) 101, 1153, 1217, 2233
NruI TCGCGA 1 cut(s) 1092
NsiI ATGCAT 1 cut(s) 1987
NspI RCATGY 1 cut(s) 1026
NspV TTCGAA 1 cut(s) 669
PceI AGGCCT 2 cut(s) 354, 1470
PctI GAATGC 1 cut(s) 1958
PfeI GAWTC 7 cut(s) 562, 826, 985, 1052, 1087, 2132, 2167
PflMI CCANNNNNTGG 1 cut(s) 173
PleI GAGTC 3 cut(s) 98, 483, 1214
PpsI GAGTC 3 cut(s) 98, 483, 1214
PpuMI RGGWCCY 1 cut(s) 1659
Psp124BI GAGCTC 1 cut(s) 920
Psp5II RGGWCCY 1 cut(s) 1659
Psp6I CCWGG 2 cut(s) 73, 1189
PspFI CCCAGC 2 cut(s) 1034, 2114
PspGI CCWGG 2 cut(s) 73, 1189
PspN4I GGNNCC 2 cut(s) 124, 2015
PspPI GGNCC 3 cut(s) 34, 1659, 2252
PspPPI RGGWCCY 1 cut(s) 1659
PstI CTGCAG 2 cut(s) 1064, 2144
PsuI RGATCY 2 cut(s) 1111, 2191
PvuII CAGCTG 3 cut(s) 472, 1151, 2231
RruI TCGCGA 1 cut(s) 1092
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SacI GAGCTC 1 cut(s) 920
Sau3AI GATC 6 cut(s) 760, 865, 1111, 1789, 1894, 2191
Sau96I GGNCC 3 cut(s) 34, 1659, 2252
SchI GAGTC 3 cut(s) 98, 484, 1214
ScrFI CCNGG 2 cut(s) 75, 1191
SduI GDGCHC 1 cut(s) 920
SfaNI GCATC 6 cut(s) 792, 797, 886, 1821, 1826, 1915
SfcI CTRYAG 3 cut(s) 48, 1060, 2140
SfuI TTCGAA 1 cut(s) 669
SinI GGWCC 3 cut(s) 34, 1659, 2252
SmiI ATTTAAAT 1 cut(s) 1890
SmlI CTYRAG 2 cut(s) 1376, 1915
SmoI CTYRAG 2 cut(s) 1376, 1915
SseBI AGGCCT 2 cut(s) 354, 1470
SspI AATATT 1 cut(s) 674
SspMI CTAG 3 cut(s) 98, 153, 1269
SstI GAGCTC 1 cut(s) 920
StuI AGGCCT 2 cut(s) 354, 1470
StyD4I CCNGG 2 cut(s) 73, 1189
StyI CCWWGG 1 cut(s) 2292
SwaI ATTTAAAT 1 cut(s) 1890
TaaI ACNGT 8 cut(s) 580, 689, 1049, 1346, 1609, 1718, 2048, 2129
TaiI ACGT 3 cut(s) 120, 1418, 1965
TaqI TCGA 4 cut(s) 669, 960, 1788, 2043
TaqII GACCGA 2 cut(s) 245, 1361
TauI GCSGC 3 cut(s) 277, 1393, 2216
TfiI GAWTC 7 cut(s) 562, 826, 985, 1052, 1087, 2132, 2167
TscAI CASTG 5 cut(s) 208, 1324, 1351, 1503, 1612
TseFI GTSAC 4 cut(s) 101, 1153, 1217, 2233
TseI GCWGC 8 cut(s) 515, 810, 1019, 1133, 1148, 1544, 2099, 2228
Tsp45I GTSAC 4 cut(s) 101, 1153, 1217, 2233
TspDTI ATGAA 9 cut(s) 54, 398, 497, 631, 750, 1198, 1526, 1779, 2189
TspGWI ACGGA 2 cut(s) 514, 1543
TspRI CASTG 5 cut(s) 208, 1324, 1351, 1503, 1612
Van91I CCANNNNNTGG 1 cut(s) 173
VpaK11BI GGWCC 3 cut(s) 34, 1659, 2252
XapI RAATTY 4 cut(s) 707, 751, 1736, 2275
XceI RCATGY 1 cut(s) 1026
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XspI CTAG 3 cut(s) 98, 153, 1269
ZraI GACGTC 1 cut(s) 1963
Zsp2I ATGCAT 1 cut(s) 1987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.