MD10G1131900.v1.1

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000448
Physical Location & Seq
Reverse (-)
21213009 .. 21214850
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1131900.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGCAAACAAGTCATGGGTTGTTCTTTCTCTGGTCCTATGTGTGGCTGTGGCTGTGGCTGTGGCTGTTGTTAGTGCATCAGACCCAGGTCTGCAACTAATCTTCGTACTAGGGGACTCAACTGCTGACGTTGGAACCAATAACTTCTTGCCCACCAGCATGGTTAGGGCTGACTTTCCTCATAATGGCATTGACTTTCCTTCCTCAAAACCCACTGGAAGGTTCAGCAACGGCCTCAATAGTGCTGATTTTCTTGCTCAGATGCTCGGTCTCAAGAGAAGTCCGCCGCCTTTTCTTTCTCTCAACGCGAAGTCTCTTCGCAAGAAGAGGTTTAGTGGTGTTAACTTTGCTTCTGGAGGGTCAGGCCTTCTTGACATAACTGGAAAAACATCGCTGACTCTGATGAAGTTTGGAACACCAGTGAAAACTCCGGTTGTTGCCCCATCCGGCAATCGACAAAATGTTGTGTCATTAACAGAGCAGATACAACAATTTGCCACTGTCAAGAGAAATCTTACGGCCATAAAAGGGGGGAGGAGAGTAACTGAGAAGTCTTTGTTCTTCATCAGCACCGGTAGCAATGACCTTTTCGGATATTACCACTCAAACAGTTCCATTCCAAAGGAAGAGTTCTTATCTTCTTTAGAGCTTGCTTATGAGAACCACCTGAAGAGCTTAATCGATCTTGGAGCGAGGAAATTTGGCATCATTAGCGTTGCCCCCATTGGATGCTGCCCATCTCAAAGGATTCACAACACTACCGGCGGATGTTTGGAGGGTTTGAACGAGCTTGCAGTAGCTTTCCATGCAAGGTTGGGTGCTCTAATGCTGAAGCTCAGCTCAGAATGTAAGGACATCAAGTATGCACTTGGGAATGCATTTGAAATGACAGTAAATGTCATTCAGAATCCTTTTCCATTCAATTTCACGCAGGTGGAAGCTGCATGTTGCGGAGCTGGGAAGCTCAACGCTGAATCCTTCTGCATTCCAAGTGCCAAACTTTGCTCGAATCGCAACAACTACTTGTTCTGGGATTTATTTCATCCAACACAGGCTGCTTCCAAGTTAGCAGCTGTAACCCTCTTCAATGGTGGAACACAATTCGTAACCCCGATTAATTTTGCTCAGTTGGCCAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

40.72

Weight (kDa)

9.34

Isoelectric Point (pI)

28.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 358 7.3e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 922
Acc36I ACCTGC 1 cut(s) 922
AccII CGCG 1 cut(s) 308
AciI CCGC 4 cut(s) 284, 287, 765, 951
AcoI YGGCCR 2 cut(s) 519, 1131
AcsI RAATTY 1 cut(s) 698
AcuI CTGAAG 2 cut(s) 689, 851
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 4 cut(s) 43, 185, 219, 528
AgeI ACCGGT 1 cut(s) 572
AgsI TTSAA 4 cut(s) 784, 884, 922, 1087
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 932
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 2 cut(s) 275, 318
AoxI GGCC 4 cut(s) 232, 364, 519, 1131
ApeKI GCWGC 4 cut(s) 732, 941, 1055, 1070
ApoI RAATTY 1 cut(s) 698
AseI ATTAAT 1 cut(s) 1116
AsiGI ACCGGT 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 34
AvaII GGWCC 1 cut(s) 34
BalI TGGCCA 1 cut(s) 1133
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 4 cut(s) 719, 928, 1042, 1082
BccI CCATC 2 cut(s) 451, 745
BceAI ACGGC 2 cut(s) 247, 534
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 275, 318
BfaI CTAG 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 922
BisI GCNGC 5 cut(s) 287, 733, 942, 1056, 1071
BlpI GCTNAGC 1 cut(s) 836
BlsI GCNGC 5 cut(s) 288, 734, 943, 1057, 1072
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 4 cut(s) 86, 252, 714, 719
BoxI GACNNNNGTC 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 375
Bpu1102I GCTNAGC 1 cut(s) 836
BpuEI CTTGAG 1 cut(s) 257
Bsa29I ATCGAT 1 cut(s) 681
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 85, 1134
BsaWI WCCGGW 2 cut(s) 430, 572
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 185, 219, 528
Bse118I RCCGGY 2 cut(s) 572, 761
Bse1I ACTGG 3 cut(s) 220, 385, 419
Bse3DI GCAATG 1 cut(s) 586
BseBI CCWGG 1 cut(s) 87
BseCI ATCGAT 1 cut(s) 681
BseDI CCNNGG 2 cut(s) 85, 1134
BseGI GGATG 4 cut(s) 443, 734, 773, 1042
BseLI CCNNNNNNNGG 4 cut(s) 43, 185, 219, 528
BseMI GCAATG 1 cut(s) 586
BseMII CTCAG 5 cut(s) 272, 537, 850, 855, 1139
BseNI ACTGG 3 cut(s) 220, 385, 419
BseRI GAGGAG 1 cut(s) 550
BseXI GCAGC 4 cut(s) 719, 928, 1042, 1082
BseYI CCCAGC 1 cut(s) 956
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 4 cut(s) 234, 366, 521, 1133
BshTI ACCGGT 1 cut(s) 572
BshVI ATCGAT 1 cut(s) 681
BsiHKAI GWGCWC 1 cut(s) 823
BsiSI CCGG 4 cut(s) 431, 447, 573, 762
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 4 cut(s) 43, 185, 219, 528
BsmAI GTCTC 2 cut(s) 275, 318
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 2 cut(s) 880, 984
BsnI GGCC 4 cut(s) 234, 366, 521, 1133
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 823
Bsp143I GATC 1 cut(s) 682
Bsp1720I GCTNAGC 1 cut(s) 836
BspACI CCGC 4 cut(s) 284, 287, 765, 951
BspANI GGCC 4 cut(s) 234, 366, 521, 1133
BspCNI CTCAG 5 cut(s) 271, 538, 849, 854, 1138
BspDI ATCGAT 1 cut(s) 681
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 136
BspMI ACCTGC 1 cut(s) 922
BspQI GCTCTTC 1 cut(s) 665
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 586
BsrFI RCCGGY 2 cut(s) 572, 761
BsrI ACTGG 3 cut(s) 220, 385, 419
BssAI RCCGGY 2 cut(s) 572, 761
BssECI CCNNGG 2 cut(s) 85, 1134
BssMI GATC 1 cut(s) 682
BssT1I CCWWGG 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 3 cut(s) 502, 611, 892
Bst6I CTCTTC 5 cut(s) 320, 321, 621, 665, 1088
BstC8I GCNNGC 2 cut(s) 651, 792
BstDEI CTNAG 5 cut(s) 258, 546, 836, 841, 1125
BstF5I GGATG 4 cut(s) 443, 734, 773, 1042
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 1 cut(s) 685
BstMAI GTCTC 2 cut(s) 275, 318
BstMBI GATC 1 cut(s) 682
BstMWI GCNNNNNNNGC 5 cut(s) 576, 711, 806, 1011, 1130
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 948
BstPAI GACNNNNGTC 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 4 cut(s) 719, 928, 1042, 1082
BstXI CCANNNNNNTGG 1 cut(s) 160
Bsu15I ATCGAT 1 cut(s) 681
BsuRI GGCC 4 cut(s) 234, 366, 521, 1133
BsuTUI ATCGAT 1 cut(s) 681
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 4 cut(s) 443, 734, 773, 1042
BtsIMutI CAGTG 3 cut(s) 213, 426, 498
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 2 cut(s) 651, 792
Cfr10I RCCGGY 2 cut(s) 572, 761
Cfr13I GGNCC 1 cut(s) 34
ClaI ATCGAT 1 cut(s) 681
Csp6I GTAC 1 cut(s) 107
CspAI ACCGGT 1 cut(s) 572
CviAII CATG 4 cut(s) 15, 160, 806, 945
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 5 cut(s) 258, 546, 836, 841, 1125
DpnI GATC 1 cut(s) 684
DpnII GATC 1 cut(s) 682
EaeI YGGCCR 2 cut(s) 519, 1131
Eam1104I CTCTTC 5 cut(s) 320, 321, 621, 665, 1088
EarI CTCTTC 5 cut(s) 320, 321, 621, 665, 1088
EciI GGCGGA 2 cut(s) 273, 780
Eco130I CCWWGG 1 cut(s) 1134
Eco147I AGGCCT 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 275
Eco47I GGWCC 1 cut(s) 34
Eco57I CTGAAG 2 cut(s) 689, 851
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 1134
EcoT22I ATGCAT 1 cut(s) 880
ErhI CCWWGG 1 cut(s) 1134
FaeI CATG 4 cut(s) 18, 163, 809, 948
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 14, 159, 805, 944
Fnu4HI GCNGC 5 cut(s) 287, 733, 942, 1056, 1071
FokI GGATG 4 cut(s) 430, 741, 780, 1029
Fsp4HI GCNGC 5 cut(s) 287, 733, 942, 1056, 1071
FspBI CTAG 1 cut(s) 110
GluI GCNGC 5 cut(s) 287, 733, 942, 1056, 1071
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 4 cut(s) 234, 366, 521, 1133
HapII CCGG 4 cut(s) 431, 447, 573, 762
Hin1II CATG 4 cut(s) 18, 163, 809, 948
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 6 cut(s) 116, 397, 748, 907, 974, 1009
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 4 cut(s) 431, 447, 573, 762
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 6 cut(s) 82, 261, 402, 593, 844, 906
Hpy188III TCNNGA 4 cut(s) 274, 354, 371, 505
Hpy8I GTNNAC 1 cut(s) 343
HpyAV CCTTC 4 cut(s) 210, 213, 377, 988
HpyCH4III ACNGT 3 cut(s) 502, 611, 892
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 8 cut(s) 77, 94, 794, 809, 866, 878, 944, 984
HpyF10VI GCNNNNNNNGC 5 cut(s) 576, 711, 806, 1011, 1130
HpyF3I CTNAG 5 cut(s) 258, 546, 836, 841, 1125
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 4 cut(s) 18, 163, 809, 948
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 1 cut(s) 682
LguI GCTCTTC 1 cut(s) 665
LmnI GCTCC 2 cut(s) 689, 953
Lsp1109I GCAGC 4 cut(s) 719, 928, 1042, 1082
LweI GCATC 4 cut(s) 86, 252, 714, 719
MaeI CTAG 1 cut(s) 110
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 541, 1075, 1105
MalI GATC 1 cut(s) 684
MboI GATC 1 cut(s) 682
MboII GAAGA 8 cut(s) 94, 308, 337, 553, 630, 638, 682, 1075
MhlI GDGCHC 1 cut(s) 823
MlsI TGGCCA 1 cut(s) 1133
MluCI AATT 5 cut(s) 491, 698, 922, 1100, 1117
MluNI TGGCCA 1 cut(s) 1133
MlyI GAGTC 2 cut(s) 110, 391
MmeI TCCRAC 2 cut(s) 112, 1070
MnlI CCTC 9 cut(s) 189, 214, 245, 321, 350, 528, 687, 769, 1091
Mox20I TGGCCA 1 cut(s) 1133
Mph1103I ATGCAT 1 cut(s) 880
MscI TGGCCA 1 cut(s) 1133
MseI TTAA 5 cut(s) 342, 473, 677, 1116, 1141
MslI CAYNNNNRTG 2 cut(s) 158, 932
Msp20I TGGCCA 1 cut(s) 1133
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 4 cut(s) 431, 447, 573, 762
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 2 cut(s) 880, 984
MvaI CCWGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 5 cut(s) 576, 711, 806, 1011, 1130
NdeII GATC 1 cut(s) 682
NlaIII CATG 4 cut(s) 18, 163, 809, 948
NlaIV GGNNCC 1 cut(s) 136
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 1 cut(s) 948
OliI CACNNNNGTG 1 cut(s) 932
PaqCI CACCTGC 1 cut(s) 922
PceI AGGCCT 1 cut(s) 366
PciSI GCTCTTC 1 cut(s) 665
PctI GAATGC 2 cut(s) 880, 984
PfeI GAWTC 4 cut(s) 748, 907, 974, 1009
PinAI ACCGGT 1 cut(s) 572
PkrI GCNGC 5 cut(s) 288, 734, 943, 1057, 1072
PleI GAGTC 2 cut(s) 110, 391
PpsI GAGTC 2 cut(s) 110, 391
PshAI GACNNNNGTC 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 1116
Psp6I CCWGG 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 136
PspPI GGNCC 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 2 cut(s) 158, 932
SapI GCTCTTC 1 cut(s) 665
SaqAI TTAA 5 cut(s) 342, 473, 677, 1116, 1141
SatI GCNGC 5 cut(s) 287, 733, 942, 1056, 1071
Sau3AI GATC 1 cut(s) 682
Sau96I GGNCC 1 cut(s) 34
SchI GAGTC 2 cut(s) 110, 391
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 823
SfaNI GCATC 4 cut(s) 86, 252, 714, 719
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 2 cut(s) 158, 932
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 5 cut(s) 491, 698, 922, 1100, 1117
SseBI AGGCCT 1 cut(s) 366
SsiI CCGC 4 cut(s) 284, 287, 765, 951
SspMI CTAG 1 cut(s) 110
StuI AGGCCT 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 3 cut(s) 502, 611, 892
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 454, 681, 1007
TaqII GACCGA 1 cut(s) 257
TasI AATT 5 cut(s) 491, 698, 922, 1100, 1117
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 4 cut(s) 748, 907, 974, 1009
Tru1I TTAA 5 cut(s) 342, 473, 677, 1116, 1141
Tru9I TTAA 5 cut(s) 342, 473, 677, 1116, 1141
TscAI CASTG 3 cut(s) 220, 426, 505
TseI GCWGC 4 cut(s) 732, 941, 1055, 1070
TspDTI ATGAA 3 cut(s) 419, 553, 1031
TspRI CASTG 3 cut(s) 220, 426, 505
VpaK11BI GGWCC 1 cut(s) 34
VspI ATTAAT 1 cut(s) 1116
XapI RAATTY 1 cut(s) 698
XceI RCATGY 1 cut(s) 948
XspI CTAG 1 cut(s) 110
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.