RchiOBHm_Chr6g0257301

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000448
Physical Location & Seq
Reverse (-)
12590429 .. 12593324
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23079

Sequence Viewer

Length: 1110 bp
ATGGCAAAGTCGAAGCTGATGATGCTTTTTGTTTCATTTTGTTTGTTATCTGTGTTTAGTGCATCTGAGGTAGCTCCGCCTGCGGCCATTTTCATATTTGGAGACTCAACTGTGGATGTTGGCACCAATACCTTGTTGCTCCAAAGTGGAGCAAGGGCTGATTTCTCTCCAAATGGGGTTGATTTTCCTTATTCTGTGCCTACTGGGAGGTTCAGCAATGGCCTTAACAGCGCTGACCAAATCGTGAAACTATTTGGACGCCGGAAAAGTCCACCACCTTTCTTGTATGCCGCGAGTCATATGTCCACTTTTAAAAGGAATATACTGCAGGGAGTCAACTTTGCTTCAGCAGGAAGTGGCATCTTCCAAGACACGGGGATAAAAAGATGGACAGAAGTTGTGTCACTGGGAAATCAGATCCAACAATTTGCAGCGGTGCGTGGAAATTTCACAGAGATAGTTGGTTTCAAAACAACTGATACCGTGCTTTCCAAGTCTTTGTTCATCATCAGTATCGGAAGCAACGATCTTTTCGAATTAGTTGAGTACTTTCCAAATGCCACGGACTTGTTTAAAGCGGAACACATGGAACGTCTTCAACTAACGTATAAGAACCATTTGAAGAATTTATACAAGCTGGGAGCTCGGAAATTCGGGATTATAAGTGTTCCTCCAATTGGATGTTGTCCATATGCCCGTGTTCAACCAAATGTCGACCCTAGTGTTTGTGTGAAGGAACTAAACAAGCTTGCTCAAACATTTTTCATAGAAACACACGCTCTCCTGCGGAAATTGAGCTCGGATTTGAAAGGGATGAGGTATTCACTTGGAAATGCCTATGAAATGACCATGAGTATCATTCAAGACCCATTAGCCTTTGGGTTTAAGGACGTTCAATCAGCCTGCTGTGGTTACGGGAGGTTAAATGGAGAAAAAGCATGCGTTACAATTTTTGACCCCAACCTTTGTTCGAATCGTCAGGAGTTCCTATTCTGGGACTTGTATCATCCTACTGAGTTTGCGTCGCAACTAGCAGCACGAACACTTTATGGTGCAGGAACAAGATATATGACACCCATGAACTTCAGTCAGTTGGTGGCTAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.88

Weight (kDa)

8.91

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 347 4.4e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 714
AccII CGCG 1 cut(s) 293
AciI CCGC 6 cut(s) 77, 83, 291, 434, 578, 787
AclWI GGATC 1 cut(s) 412
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 3 cut(s) 445, 625, 650
AcuI CTGAAG 2 cut(s) 330, 1069
AcyI GRCGYC 1 cut(s) 259
AfaI GTAC 1 cut(s) 548
AfeI AGCGCT 1 cut(s) 232
AfiI CCNNNNNNNGG 3 cut(s) 373, 677, 994
AgsI TTSAA 7 cut(s) 469, 599, 622, 704, 808, 863, 896
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 7 cut(s) 16, 74, 637, 644, 748, 798, 1104
AluI AGCT 7 cut(s) 16, 74, 637, 644, 748, 798, 1104
Alw21I GWGCWC 2 cut(s) 646, 800
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 1 cut(s) 412
Aor51HI AGCGCT 1 cut(s) 232
AoxI GGCC 2 cut(s) 84, 220
ApeKI GCWGC 2 cut(s) 431, 1034
ApoI RAATTY 3 cut(s) 445, 625, 650
Asp700I GAANNNNTTC 1 cut(s) 594
AspLEI GCGC 1 cut(s) 233
AsuII TTCGAA 2 cut(s) 534, 971
AsuNHI GCTAGC 1 cut(s) 1100
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 2 cut(s) 646, 800
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
BbsI GAAGAC 1 cut(s) 587
Bbv12I GWGCWC 2 cut(s) 646, 800
BbvI GCAGC 2 cut(s) 443, 1046
BccI CCATC 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 3 cut(s) 720, 1031, 1101
BfmI CTRYAG 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 234
BisI GCNGC 4 cut(s) 84, 291, 432, 1035
BlsI GCNGC 4 cut(s) 85, 292, 433, 1036
BmcAI AGTACT 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 124
BmrI ACTGGG 2 cut(s) 213, 416
BmsI GCATC 3 cut(s) 12, 71, 369
BmtI GCTAGC 1 cut(s) 1104
BmuI ACTGGG 2 cut(s) 213, 416
BpiI GAAGAC 1 cut(s) 587
Bpu14I TTCGAA 2 cut(s) 534, 971
BsaHI GRCGYC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 561
BsaXI ACNNNNNCTCC 2 cut(s) 765, 795
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 677, 994
Bse1I ACTGG 2 cut(s) 208, 411
Bse3DI GCAATG 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 4 cut(s) 121, 686, 819, 1006
BseLI CCNNNNNNNGG 3 cut(s) 373, 677, 994
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 2 cut(s) 57, 1005
BseNI ACTGG 2 cut(s) 208, 411
BseXI GCAGC 2 cut(s) 443, 1046
BseYI CCCAGC 1 cut(s) 637
BsgI GTGCAG 1 cut(s) 1074
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 2 cut(s) 86, 222
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 2 cut(s) 646, 800
BsiSI CCGG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 3 cut(s) 373, 677, 994
BsmAI GTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 1010
BsnI GGCC 2 cut(s) 86, 222
Bsp119I TTCGAA 2 cut(s) 534, 971
Bsp1286I GDGCHC 2 cut(s) 646, 800
Bsp143I GATC 2 cut(s) 417, 526
BspACI CCGC 6 cut(s) 77, 83, 291, 434, 578, 787
BspANI GGCC 2 cut(s) 86, 222
BspCNI CTCAG 2 cut(s) 58, 1006
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 1 cut(s) 124
BspMAI CTGCAG 1 cut(s) 330
BspOI GCTAGC 1 cut(s) 1104
BspPI GGATC 1 cut(s) 412
BspT104I TTCGAA 2 cut(s) 534, 971
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 223
BsrI ACTGG 2 cut(s) 208, 411
BssECI CCNNGG 1 cut(s) 561
BssMI GATC 2 cut(s) 417, 526
BssNI GRCGYC 1 cut(s) 259
Bst4CI ACNGT 2 cut(s) 112, 484
BstACI GRCGYC 1 cut(s) 259
BstBI TTCGAA 2 cut(s) 534, 971
BstC8I GCNNGC 5 cut(s) 81, 750, 904, 940, 1102
BstDEI CTNAG 2 cut(s) 66, 1014
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 4 cut(s) 121, 686, 819, 1006
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 2 cut(s) 420, 529
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 2 cut(s) 417, 526
BstMWI GCNNNNNNNGC 3 cut(s) 22, 80, 228
BstNSI RCATGY 1 cut(s) 942
BstSFI CTRYAG 1 cut(s) 326
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 2 cut(s) 443, 1046
BstV2I GAAGAC 1 cut(s) 587
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BsuRI GGCC 2 cut(s) 86, 222
BtgI CCRYGG 1 cut(s) 561
BtsCI GGATG 4 cut(s) 121, 686, 819, 1006
BtsIMutI CAGTG 1 cut(s) 404
Cac8I GCNNGC 5 cut(s) 81, 750, 904, 940, 1102
CfoI GCGC 1 cut(s) 233
CseI GACGC 2 cut(s) 267, 1011
Csp6I GTAC 1 cut(s) 547
CspCI CAANNNNNGTGG 2 cut(s) 264, 299
CviAII CATG 4 cut(s) 586, 850, 939, 1078
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 2 cut(s) 66, 1014
DpnI GATC 2 cut(s) 419, 528
DpnII GATC 2 cut(s) 417, 526
DraI TTTAAA 2 cut(s) 313, 574
EaeI YGGCCR 1 cut(s) 84
EciI GGCGGA 1 cut(s) 66
Ecl136II GAGCTC 2 cut(s) 644, 798
Eco24I GRGCYC 2 cut(s) 646, 800
Eco47III AGCGCT 1 cut(s) 232
Eco53kI GAGCTC 2 cut(s) 644, 798
Eco57I CTGAAG 2 cut(s) 330, 1069
EcoICRI GAGCTC 2 cut(s) 644, 798
EcoT38I GRGCYC 2 cut(s) 646, 800
FaeI CATG 4 cut(s) 589, 853, 942, 1081
FaqI GGGAC 1 cut(s) 1010
FatI CATG 4 cut(s) 585, 849, 938, 1077
FauNDI CATATG 2 cut(s) 300, 691
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 4 cut(s) 84, 291, 432, 1035
FokI GGATG 4 cut(s) 128, 693, 826, 993
FriOI GRGCYC 2 cut(s) 646, 800
Fsp4HI GCNGC 4 cut(s) 84, 291, 432, 1035
FspBI CTAG 3 cut(s) 720, 1031, 1101
GlaI GCGC 1 cut(s) 232
GluI GCNGC 4 cut(s) 84, 291, 432, 1035
GsaI CCCAGC 1 cut(s) 641
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 2 cut(s) 86, 222
HapII CCGG 1 cut(s) 262
HgaI GACGC 2 cut(s) 267, 1011
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 259
Hin1II CATG 4 cut(s) 589, 853, 942, 1081
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 2 cut(s) 337, 715
HindII GTYRAC 2 cut(s) 337, 715
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 4 cut(s) 104, 295, 333, 973
HpaII CCGG 1 cut(s) 262
Hpy166II GTNNAC 4 cut(s) 272, 306, 337, 715
Hpy188I TCNGA 5 cut(s) 67, 417, 518, 648, 802
Hpy188III TCNNGA 4 cut(s) 244, 655, 863, 980
Hpy8I GTNNAC 4 cut(s) 272, 306, 337, 715
Hpy99I CGWCG 1 cut(s) 1027
HpyAV CCTTC 1 cut(s) 727
HpyCH4III ACNGT 2 cut(s) 112, 484
HpyCH4IV ACGT 3 cut(s) 592, 605, 891
HpyCH4V TGCA 4 cut(s) 62, 328, 431, 1055
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 80, 228
HpyF3I CTNAG 2 cut(s) 66, 1014
HpySE526I ACGT 3 cut(s) 592, 605, 891
Hsp92I GRCGYC 1 cut(s) 259
Hsp92II CATG 4 cut(s) 589, 853, 942, 1081
HspAI GCGC 1 cut(s) 231
Kzo9I GATC 2 cut(s) 417, 526
LmnI GCTCC 4 cut(s) 79, 144, 149, 641
Lsp1109I GCAGC 2 cut(s) 443, 1046
LweI GCATC 3 cut(s) 12, 71, 369
MaeI CTAG 3 cut(s) 720, 1031, 1101
MaeII ACGT 3 cut(s) 592, 605, 891
MaeIII GTNAC 3 cut(s) 402, 911, 943
MalI GATC 2 cut(s) 419, 528
MboI GATC 2 cut(s) 417, 526
MboII GAAGA 3 cut(s) 355, 587, 634
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 1 cut(s) 417
MhlI GDGCHC 2 cut(s) 646, 800
MluCI AATT 8 cut(s) 425, 445, 536, 625, 650, 675, 791, 948
MlyI GAGTC 3 cut(s) 98, 304, 342
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 5 cut(s) 61, 201, 681, 810, 912
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 5 cut(s) 225, 312, 573, 885, 923
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 1 cut(s) 262
MunI CAATTG 1 cut(s) 675
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 3 cut(s) 22, 80, 228
NdeI CATATG 2 cut(s) 300, 691
NdeII GATC 2 cut(s) 417, 526
NheI GCTAGC 1 cut(s) 1100
NlaIII CATG 4 cut(s) 589, 853, 942, 1081
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 1 cut(s) 402
NspI RCATGY 1 cut(s) 942
NspV TTCGAA 2 cut(s) 534, 971
PaeI GCATGC 1 cut(s) 942
PcsI WCGNNNNNNNCGW 2 cut(s) 522, 531
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 1 cut(s) 973
PkrI GCNGC 4 cut(s) 85, 292, 433, 1036
PleI GAGTC 3 cut(s) 98, 303, 341
PpsI GAGTC 3 cut(s) 98, 303, 341
PsiI TTATAA 1 cut(s) 662
Psp124BI GAGCTC 2 cut(s) 646, 800
PspFI CCCAGC 1 cut(s) 637
PspN4I GGNNCC 1 cut(s) 124
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 1 cut(s) 417
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
SacI GAGCTC 2 cut(s) 646, 800
SalI GTCGAC 1 cut(s) 713
SaqAI TTAA 5 cut(s) 225, 312, 573, 885, 923
SatI GCNGC 4 cut(s) 84, 291, 432, 1035
Sau3AI GATC 2 cut(s) 417, 526
ScaI AGTACT 1 cut(s) 548
SchI GAGTC 3 cut(s) 98, 304, 342
SduI GDGCHC 2 cut(s) 646, 800
SfaNI GCATC 3 cut(s) 12, 71, 369
SfcI CTRYAG 1 cut(s) 326
SfuI TTCGAA 2 cut(s) 534, 971
SphI GCATGC 1 cut(s) 942
Sse9I AATT 8 cut(s) 425, 445, 536, 625, 650, 675, 791, 948
SsiI CCGC 6 cut(s) 77, 83, 291, 434, 578, 787
SspMI CTAG 3 cut(s) 720, 1031, 1101
SstI GAGCTC 2 cut(s) 646, 800
TaaI ACNGT 2 cut(s) 112, 484
TaiI ACGT 3 cut(s) 595, 608, 894
TaqI TCGA 4 cut(s) 11, 534, 714, 971
TasI AATT 8 cut(s) 425, 445, 536, 625, 650, 675, 791, 948
TatI WGTACW 1 cut(s) 546
TauI GCSGC 2 cut(s) 86, 293
TfiI GAWTC 1 cut(s) 973
Tru1I TTAA 5 cut(s) 225, 312, 573, 885, 923
Tru9I TTAA 5 cut(s) 225, 312, 573, 885, 923
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 1 cut(s) 402
TseI GCWGC 2 cut(s) 431, 1034
Tsp45I GTSAC 1 cut(s) 402
TspDTI ATGAA 6 cut(s) 24, 82, 493, 754, 855, 1094
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 1 cut(s) 411
XapI RAATTY 3 cut(s) 445, 625, 650
XceI RCATGY 1 cut(s) 942
XmiI GTMKAC 1 cut(s) 714
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 3 cut(s) 720, 1031, 1101
ZrmI AGTACT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.