Rh2BG119300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000448
Physical Location & Seq
Forward (+)
10143093 .. 10148118
5026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG119300.1

Sequence Viewer

Length: 1101 bp
ATGGCCAAGTCTGCAATATTTTCTCTTTGGTTCAATATTATTGTGTATCTCGGCATACTTGGATTGCACGCCTCACACTCTCAGCCTTGTGTGCCACCACTTTACATATTTGGTGACTCCACAGCTGATGTTGGAACCAACAATTATTTGCCCAATTCCACTGCGAGGGCCGATTCCCTTTATAACGGGATCGATTTTCCTTTGCGTAAACCAACTGGGAGATTTAGCAACGGTTACAACACTATTGATTATCTAGCCCAGCTTCTGAAATTCTACGAAAGCCCGCCGCCTTTTCTCTCGTTTTCAGACAGCAATATTTCGCATTTTCTCATCAGGACTGTTCCAACGACGGGAATCAATTTTGCTTCAGGAGGGTCGGGACTTCTGGATTCTACAGGACGTAAGTATAAAAGGGTGATTTCTTTTGGGGAACAAGTCCAACAATTTTCAAGTGTCCGGAGCAATATTTCAGAATTACTGGGTGCGAGTGCCGTCGTAAATATCTCCAAGTCTCTCTTCATTATCAGCGTTGGAAGCAATGACATCTTCGAACATTATGATGCTAACGATACTAGTACTGGGCAAAACTACATTACCACTCTCATCTCCACCTACGAAACTCATCTAAGGAATTTATATGAGCTAGGAGCCAGAAGATTTGGGATTATTAGTGTTGGGGCACTCGGGTGCTGTCCGATCCTACGTAGTAAATATGGAGGTTGTTGTTTGGAGCCCATGAATAGGGATGCTCAGTTGTTCTATACTGCACTCCGAGACCTCTTGCAAAAGTTGAGTTCAGAGTGCAGAGGGTTGATGTATGCACTTGGAGATTCATATAATATGTCAAAATATATTCTCGACCACCCCGATAAATCTGTTTTTTCTGAGATTAAAAGAGCTTGCTGTGGAAAAGGAAAGTACAACGCAGATGAGGCATGTAAACCGAATTCGGTTTTGTGTAAAGATCGCACAACCTACTTGTTTTGGGACCAGTACCATCCAACACAGGAGGCTTCTTCTCTCGCAGCCAGTATTCTTTATACTGGTACCAAACCATTCGTGGTGCCGATGGGTTTTGGTGAATTGGCCCTTCTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.4

Weight (kDa)

7.96

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 344 1e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
Acc65I GGTACC 1 cut(s) 1046
AccB1I GGYRCC 2 cut(s) 1046, 1063
AccIII TCCGGA 1 cut(s) 456
AciI CCGC 2 cut(s) 284, 287
AclWI GGATC 2 cut(s) 197, 691
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 269, 631, 946
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 4 cut(s) 577, 920, 995, 1048
AfiI CCNNNNNNNGG 3 cut(s) 165, 350, 741
AgsI TTSAA 2 cut(s) 34, 450
AhlI ACTAGT 1 cut(s) 572
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AleI CACNNNNGTG 1 cut(s) 685
AluBI AGCT 4 cut(s) 125, 262, 643, 899
AluI AGCT 4 cut(s) 125, 262, 643, 899
Alw26I GTCTC 2 cut(s) 516, 768
AlwI GGATC 2 cut(s) 197, 691
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 683
Aor13HI TCCGGA 1 cut(s) 456
AoxI GGCC 3 cut(s) 3, 168, 1086
ApeKI GCWGC 1 cut(s) 1025
ApoI RAATTY 3 cut(s) 269, 631, 946
Asp718I GGTACC 1 cut(s) 1046
AspS9I GGNCC 3 cut(s) 168, 988, 1087
AsuHPI GGTGA 3 cut(s) 125, 427, 1091
AsuII TTCGAA 1 cut(s) 549
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 1 cut(s) 988
BaeGI GKGCMC 1 cut(s) 682
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 1046, 1063
BanII GRGCYC 1 cut(s) 735
BbvI GCAGC 1 cut(s) 1037
BccI CCATC 2 cut(s) 1005, 1063
BceAI ACGGC 1 cut(s) 476
BcoDI GTCTC 2 cut(s) 516, 768
BcuI ACTAGT 1 cut(s) 572
BfaI CTAG 3 cut(s) 254, 573, 644
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 2 cut(s) 287, 1026
BlsI GCNGC 2 cut(s) 288, 1027
BmcAI AGTACT 1 cut(s) 577
Bme18I GGWCC 1 cut(s) 988
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 3 cut(s) 168, 988, 1087
BmiI GGNNCC 6 cut(s) 136, 649, 732, 989, 1048, 1065
BmrI ACTGGG 3 cut(s) 225, 488, 588
BmsI GCATC 2 cut(s) 550, 736
BmuI ACTGGG 3 cut(s) 225, 488, 588
Bpu14I TTCGAA 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 704
BsaI GGTCTC 1 cut(s) 768
BsaWI WCCGGW 1 cut(s) 456
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 350, 741
Bse1I ACTGG 6 cut(s) 220, 483, 583, 991, 1029, 1048
Bse3DI GCAATG 1 cut(s) 544
BseAI TCCGGA 1 cut(s) 456
BseCI ATCGAT 1 cut(s) 192
BseGI GGATG 2 cut(s) 751, 997
BseLI CCNNNNNNNGG 3 cut(s) 165, 350, 741
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 3 cut(s) 95, 764, 876
BseNI ACTGG 6 cut(s) 220, 483, 583, 991, 1029, 1048
BseSI GKGCMC 1 cut(s) 682
BseXI GCAGC 1 cut(s) 1037
BseYI CCCAGC 1 cut(s) 258
BsgI GTGCAG 2 cut(s) 750, 823
BshFI GGCC 3 cut(s) 5, 170, 1088
BshNI GGYRCC 2 cut(s) 1046, 1063
BshVI ATCGAT 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 1 cut(s) 457
BslFI GGGAC 2 cut(s) 393, 1001
BslI CCNNNNNNNGG 3 cut(s) 165, 350, 741
BsmAI GTCTC 2 cut(s) 516, 768
BsmFI GGGAC 2 cut(s) 393, 1001
BsnI GGCC 3 cut(s) 5, 170, 1088
Bso31I GGTCTC 1 cut(s) 768
BsoBI CYCGRG 1 cut(s) 683
Bsp119I TTCGAA 1 cut(s) 549
Bsp1286I GDGCHC 2 cut(s) 682, 735
Bsp13I TCCGGA 1 cut(s) 456
Bsp143I GATC 3 cut(s) 189, 696, 964
BspACI CCGC 2 cut(s) 284, 287
BspANI GGCC 3 cut(s) 5, 170, 1088
BspCNI CTCAG 3 cut(s) 94, 763, 877
BspDI ATCGAT 1 cut(s) 192
BspEI TCCGGA 1 cut(s) 456
BspLI GGNNCC 6 cut(s) 136, 649, 732, 989, 1048, 1065
BspPI GGATC 2 cut(s) 197, 691
BspT104I TTCGAA 1 cut(s) 549
BspT107I GGYRCC 2 cut(s) 1046, 1063
BspTNI GGTCTC 1 cut(s) 768
BsrDI GCAATG 1 cut(s) 544
BsrI ACTGG 6 cut(s) 220, 483, 583, 991, 1029, 1048
BssMI GATC 3 cut(s) 189, 696, 964
Bst4CI ACNGT 2 cut(s) 233, 340
Bst6I CTCTTC 1 cut(s) 521
BstBAI YACGTR 1 cut(s) 704
BstBI TTCGAA 1 cut(s) 549
BstC8I GCNNGC 3 cut(s) 69, 284, 901
BstDEI CTNAG 4 cut(s) 81, 626, 750, 885
BstF5I GGATG 2 cut(s) 751, 997
BstKTI GATC 3 cut(s) 192, 699, 967
BstMAI GTCTC 2 cut(s) 516, 768
BstMBI GATC 3 cut(s) 189, 696, 964
BstMWI GCNNNNNNNGC 4 cut(s) 11, 91, 534, 932
BstNSI RCATGY 1 cut(s) 939
BstSFI CTRYAG 1 cut(s) 393
BstSLI GKGCMC 1 cut(s) 682
BstSNI TACGTA 1 cut(s) 704
BstV1I GCAGC 1 cut(s) 1037
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 3 cut(s) 5, 170, 1088
BsuTUI ATCGAT 1 cut(s) 192
BtsCI GGATG 2 cut(s) 751, 997
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 3 cut(s) 69, 284, 901
CaiI CAGNNNCTG 1 cut(s) 265
Cfr13I GGNCC 3 cut(s) 168, 988, 1087
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 4 cut(s) 576, 919, 994, 1047
CviAII CATG 2 cut(s) 736, 936
CviQI GTAC 4 cut(s) 576, 919, 994, 1047
DdeI CTNAG 4 cut(s) 81, 626, 750, 885
DpnI GATC 3 cut(s) 191, 698, 966
DpnII GATC 3 cut(s) 189, 696, 964
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 521
EarI CTCTTC 1 cut(s) 521
Eco105I TACGTA 1 cut(s) 704
Eco24I GRGCYC 1 cut(s) 735
Eco31I GGTCTC 1 cut(s) 768
Eco47I GGWCC 1 cut(s) 988
Eco57I CTGAAG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 946
EcoT38I GRGCYC 1 cut(s) 735
FaeI CATG 2 cut(s) 739, 939
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 2 cut(s) 393, 1001
FatI CATG 2 cut(s) 735, 935
FauI CCCGC 1 cut(s) 291
Fnu4HI GCNGC 2 cut(s) 287, 1026
FokI GGATG 2 cut(s) 758, 984
FriOI GRGCYC 1 cut(s) 735
Fsp4HI GCNGC 2 cut(s) 287, 1026
FspBI CTAG 3 cut(s) 254, 573, 644
GluI GCNGC 2 cut(s) 287, 1026
GsaI CCCAGC 1 cut(s) 262
HaeIII GGCC 3 cut(s) 5, 170, 1088
HapII CCGG 1 cut(s) 457
Hin1II CATG 2 cut(s) 739, 939
HinfI GANTC 5 cut(s) 116, 173, 354, 389, 830
HpaII CCGG 1 cut(s) 457
HphI GGTGA 3 cut(s) 125, 427, 1091
Hpy166II GTNNAC 2 cut(s) 209, 941
Hpy188I TCNGA 7 cut(s) 267, 307, 472, 696, 773, 799, 886
Hpy188III TCNNGA 6 cut(s) 334, 369, 378, 386, 457, 857
Hpy8I GTNNAC 2 cut(s) 209, 941
Hpy99I CGWCG 2 cut(s) 352, 497
HpyAV CCTTC 1 cut(s) 1100
HpyCH4III ACNGT 2 cut(s) 233, 340
HpyCH4IV ACGT 2 cut(s) 400, 703
HpyCH4V TGCA 6 cut(s) 14, 67, 767, 784, 804, 821
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 91, 534, 932
HpyF3I CTNAG 4 cut(s) 81, 626, 750, 885
HpySE526I ACGT 2 cut(s) 400, 703
Hsp92II CATG 2 cut(s) 739, 939
Kpn2I TCCGGA 1 cut(s) 456
KpnI GGTACC 1 cut(s) 1050
Kzo9I GATC 3 cut(s) 189, 696, 964
LmnI GCTCC 3 cut(s) 459, 647, 730
Lsp1109I GCAGC 1 cut(s) 1037
LweI GCATC 2 cut(s) 550, 736
MaeI CTAG 3 cut(s) 254, 573, 644
MaeII ACGT 2 cut(s) 400, 703
MaeIII GTNAC 2 cut(s) 113, 233
MalI GATC 3 cut(s) 191, 698, 966
MboI GATC 3 cut(s) 189, 696, 964
MboII GAAGA 4 cut(s) 508, 538, 666, 1008
MhlI GDGCHC 2 cut(s) 682, 735
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 142, 154, 269, 358, 443, 473, 631, 946, 1082
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 5 cut(s) 112, 368, 463, 511, 1025
MnlI CCTC 8 cut(s) 82, 159, 365, 710, 788, 800, 925, 1003
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 456
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 891
MslI CAYNNNNRTG 2 cut(s) 558, 685
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 1 cut(s) 457
MwoI GCNNNNNNNGC 4 cut(s) 11, 91, 534, 932
NdeII GATC 3 cut(s) 189, 696, 964
NlaIII CATG 2 cut(s) 739, 939
NlaIV GGNNCC 6 cut(s) 136, 649, 732, 989, 1048, 1065
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 113
NspI RCATGY 1 cut(s) 939
NspV TTCGAA 1 cut(s) 549
OliI CACNNNNGTG 1 cut(s) 685
PcsI WCGNNNNNNNCGW 1 cut(s) 864
PfeI GAWTC 4 cut(s) 173, 354, 389, 830
PkrI GCNGC 2 cut(s) 288, 1027
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
Ppu21I YACGTR 1 cut(s) 704
PsiI TTATAA 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 258
PspN4I GGNNCC 6 cut(s) 136, 649, 732, 989, 1048, 1065
PspPI GGNCC 3 cut(s) 168, 988, 1087
PstNI CAGNNNCTG 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 4 cut(s) 577, 920, 995, 1048
RsaNI GTAC 4 cut(s) 576, 919, 994, 1047
RseI CAYNNNNRTG 2 cut(s) 558, 685
SaqAI TTAA 1 cut(s) 891
SatI GCNGC 2 cut(s) 287, 1026
Sau3AI GATC 3 cut(s) 189, 696, 964
Sau96I GGNCC 3 cut(s) 168, 988, 1087
ScaI AGTACT 1 cut(s) 577
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 2 cut(s) 682, 735
SfaNI GCATC 2 cut(s) 550, 736
SfcI CTRYAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 549
SinI GGWCC 1 cut(s) 988
SmiMI CAYNNNNRTG 2 cut(s) 558, 685
SnaBI TACGTA 1 cut(s) 704
SpeI ACTAGT 1 cut(s) 572
Sse9I AATT 9 cut(s) 142, 154, 269, 358, 443, 473, 631, 946, 1082
SsiI CCGC 2 cut(s) 284, 287
SspI AATATT 4 cut(s) 18, 37, 316, 466
SspMI CTAG 3 cut(s) 254, 573, 644
TaaI ACNGT 2 cut(s) 233, 340
TaiI ACGT 2 cut(s) 403, 706
TaqI TCGA 3 cut(s) 192, 549, 858
TasI AATT 9 cut(s) 142, 154, 269, 358, 443, 473, 631, 946, 1082
TatI WGTACW 2 cut(s) 575, 918
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 4 cut(s) 173, 354, 389, 830
Tru1I TTAA 1 cut(s) 891
Tru9I TTAA 1 cut(s) 891
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 1 cut(s) 1025
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 3 cut(s) 508, 752, 822
TspRI CASTG 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 988
XapI RAATTY 3 cut(s) 269, 631, 946
XceI RCATGY 1 cut(s) 939
XcmI CCANNNNNNNNNTGG 1 cut(s) 1057
XspI CTAG 3 cut(s) 254, 573, 644
ZrmI AGTACT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.