Rorug02G0069400

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000448
Physical Location & Seq
Forward (+)
5401025 .. 5403698
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0069400.1

Sequence Viewer

Length: 1371 bp
ATGGCTTTCCCCAAATGGGTTTCTTTGATTTTCATACTCTGTATGATTACGCCACTGCCCATTTCCTCAAACCAATCTTCGAGCCCAGAAGTGGAGCTGGCTCAAATCCCAGTCTTGTTTCTTGAACATGCTAAAAGAAACGAGCTTTTCGATCGTATGGTGAGTATCAGAAGGAAAATCCATGAAAACCCAGAGCTTTGTTATGAGGAATTTGAAACCAGTAAGCTTGTCAGAGCTGAACTTGACCGGCTGGGCATCCCTTACAGATACCCTTTGGCTGAAACCGGCGTTGTTGGCCTTGTTGGGACGGGTGGTCCACCCTTTGTTGCAATCAGAGCAGACATGGATGCTTTGGCTATGCAGGAGAGTGTGGAGTGGGAGCACAAGAGTAAAATTCCAGGGAAGATGCATGGATGTGGACATGATGCTCATGTTGCCATGCTTCTTGGAGCTGCAAGTATACTTCAGGAGTATCGCCACAAATTACAGGGGACTGTTCTTCTTGTTTTCCAACCAGCTGAGGAAGGAGGCTCAGGAGCAAAGAAAATGATAGATGAAGGTGCAGTGGAGCATGTCAGTGCCATCTTTGGGTTACATGTCGCTGCTGAGCACCCCATTGGTTCAGTGGCCTCCAGACCTGGCCCTATTTTGGCTGCGAGTGGATTTTTTGAAGCCGTAATAAGTGGGAAGGGGGGTCATGCTGCAATTCCACAGCACACAATAGACCCAATCTTGGCTGCTTCCAATGTTATTGTTAGTCTGCAACATCTTGTTTCACGTGAAGCCGACCCCTTGGATTCACAGGTTGTGACTGTTGGAAAATTCCACGGAGGTGGTGCATTTAATGTTATTCCAGATTCTGTTACAATTGGTGGTACTTTCAGGGCATTTTCAATGGAGAGCTTTATCCAACTTAAACAAAGAATCGAAGAGGTTATTACTAGACAAGCCAGTGTTCAGAGGTGCAATGCAACTGTCTTATTCAATGAAATTGCGCAGCCCTTTTACCCTGCAACTGTAAATGACAAAGATTTGCACGAGTACTTCCGGAAAGTTGCAGTCGATTTGGTGGGCCCTCAGAATGTATTTGAGAAGCAACCACTGATGGGGGCGGAAGACTTCTCATTCTATCAAGAAGTAATTCCAGGGTACTTCTTCTTTCTTGGTATGAAGAATGACTCTCAAGGACAGTTCAAACCAATGCATTCACCATATTTCAGAGTAAACGAAGACGTGCTTCCTTATGGAGCTGCATTCCATGCATCACTGGCCGCCAGGTACCTCCTGGAAAAGCAACCGAAATCTACCTCAACAGCAGACCAAAAAACGAAATCCACTTCAATGAAAGGGAACTTTCATGATGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

456

Amino Acids

50.15

Weight (kDa)

5.98

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 110 - 425 7.1e-44 Peptidase family M20/M25/M40
M20_dimer PF07687 224 - 316 3e-11 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 996
Acc65I GGTACC 1 cut(s) 1278
AccB1I GGYRCC 1 cut(s) 1278
AccI GTMKAC 1 cut(s) 460
AccIII TCCGGA 1 cut(s) 1047
AciI CCGC 2 cut(s) 1112, 1272
AcoI YGGCCR 1 cut(s) 1269
AcsI RAATTY 3 cut(s) 209, 393, 821
AcuI CTGAAG 1 cut(s) 449
AcvI CACGTG 1 cut(s) 779
AfaI GTAC 4 cut(s) 877, 1043, 1151, 1280
AfiI CCNNNNNNNGG 6 cut(s) 16, 91, 588, 649, 733, 1106
AflIII ACRYGT 1 cut(s) 595
AgsI TTSAA 7 cut(s) 125, 215, 671, 894, 985, 1195, 1341
AhdI GACNNNNNGTC 1 cut(s) 312
AjiI CACGTC 1 cut(s) 1234
AjnI CCWGG 5 cut(s) 397, 637, 1144, 1274, 1284
AleI CACNNNNGTG 1 cut(s) 831
Alw21I GWGCWC 2 cut(s) 384, 612
AlwNI CAGNNNCTG 1 cut(s) 860
Aor13HI TCCGGA 1 cut(s) 1047
AoxI GGCC 5 cut(s) 295, 627, 640, 1072, 1269
ApaI GGGCCC 1 cut(s) 1076
ApeKI GCWGC 7 cut(s) 452, 602, 653, 701, 737, 997, 1250
ApoI RAATTY 3 cut(s) 209, 393, 821
Asp700I GAANNNNTTC 1 cut(s) 1140
Asp718I GGTACC 1 cut(s) 1278
AspLEI GCGC 1 cut(s) 997
AspS9I GGNCC 4 cut(s) 314, 641, 1072, 1073
AsuHPI GGTGA 2 cut(s) 172, 1200
AvaII GGWCC 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 1076
BanI GGYRCC 1 cut(s) 1278
BanII GRGCYC 2 cut(s) 86, 1076
BauI CACGAG 1 cut(s) 1037
BbrPI CACGTG 1 cut(s) 779
BbsI GAAGAC 2 cut(s) 1122, 1236
Bbv12I GWGCWC 2 cut(s) 384, 612
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 7 cut(s) 439, 589, 640, 688, 724, 1009, 1237
BccI CCATC 2 cut(s) 590, 1099
BceAI ACGGC 1 cut(s) 659
BciT130I CCWGG 5 cut(s) 399, 639, 1146, 1276, 1286
BfaI CTAG 1 cut(s) 942
BisI GCNGC 8 cut(s) 453, 603, 654, 702, 738, 998, 1251, 1272
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 8 cut(s) 454, 604, 655, 703, 739, 999, 1252, 1273
BmcAI AGTACT 1 cut(s) 1043
Bme1390I CCNGG 5 cut(s) 399, 639, 1146, 1276, 1286
Bme18I GGWCC 1 cut(s) 314
BmeRI GACNNNNNGTC 1 cut(s) 312
BmgBI CACGTC 1 cut(s) 1234
BmgT120I GGNCC 4 cut(s) 314, 641, 1072, 1073
BmiI GGNNCC 2 cut(s) 1074, 1280
BmrFI CCNGG 5 cut(s) 399, 639, 1146, 1276, 1286
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 5 cut(s) 264, 337, 396, 415, 1271
BmuI ACTGGG 1 cut(s) 104
BpiI GAAGAC 2 cut(s) 1122, 1236
BpmI CTGGAG 1 cut(s) 616
Bpu10I CCTNAGC 2 cut(s) 519, 532
Bpu1102I GCTNAGC 1 cut(s) 606
BpuEI CTTGAG 1 cut(s) 1167
BsaAI YACGTR 1 cut(s) 779
BsaJI CCNNGG 4 cut(s) 398, 792, 826, 1145
BsaWI WCCGGW 1 cut(s) 1047
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 91, 588, 649, 733, 1106
Bse118I RCCGGY 2 cut(s) 246, 284
Bse1I ACTGG 4 cut(s) 110, 219, 951, 1272
Bse3DI GCAATG 1 cut(s) 973
BseAI TCCGGA 1 cut(s) 1047
BseBI CCWGG 5 cut(s) 399, 639, 1146, 1276, 1286
BseDI CCNNGG 4 cut(s) 398, 792, 826, 1145
BseGI GGATG 3 cut(s) 255, 352, 419
BseLI CCNNNNNNNGG 6 cut(s) 16, 91, 588, 649, 733, 1106
BseMI GCAATG 1 cut(s) 973
BseMII CTCAG 4 cut(s) 510, 546, 597, 1091
BseNI ACTGG 4 cut(s) 110, 219, 951, 1272
BseSI GKGCMC 1 cut(s) 1076
BseXI GCAGC 7 cut(s) 439, 589, 640, 688, 724, 1009, 1237
BseYI CCCAGC 1 cut(s) 250
BsgI GTGCAG 1 cut(s) 582
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 5 cut(s) 297, 629, 642, 1074, 1271
BshNI GGYRCC 1 cut(s) 1278
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 384, 612
BsiSI CCGG 3 cut(s) 247, 285, 1048
BslFI GGGAC 2 cut(s) 319, 505
BslI CCNNNNNNNGG 6 cut(s) 16, 91, 588, 649, 733, 1106
BsmFI GGGAC 2 cut(s) 319, 505
BsmI GAATGC 2 cut(s) 1204, 1253
BsnI GGCC 5 cut(s) 297, 629, 642, 1074, 1271
Bsp120I GGGCCC 1 cut(s) 1072
Bsp1286I GDGCHC 4 cut(s) 86, 384, 612, 1076
Bsp13I TCCGGA 1 cut(s) 1047
Bsp143I GATC 1 cut(s) 151
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 2 cut(s) 1112, 1272
BspANI GGCC 5 cut(s) 297, 629, 642, 1074, 1271
BspCNI CTCAG 4 cut(s) 511, 545, 598, 1090
BspEI TCCGGA 1 cut(s) 1047
BspHI TCATGA 1 cut(s) 1357
BspLI GGNNCC 2 cut(s) 1074, 1280
BspT107I GGYRCC 1 cut(s) 1278
BsrDI GCAATG 1 cut(s) 973
BsrFI RCCGGY 2 cut(s) 246, 284
BsrI ACTGG 4 cut(s) 110, 219, 951, 1272
BssAI RCCGGY 2 cut(s) 246, 284
BssECI CCNNGG 4 cut(s) 398, 792, 826, 1145
BssMI GATC 1 cut(s) 151
BssNAI GTATAC 1 cut(s) 461
BssSI CACGAG 1 cut(s) 1037
BssT1I CCWWGG 1 cut(s) 792
Bst1107I GTATAC 1 cut(s) 461
Bst2BI CACGAG 1 cut(s) 1037
Bst2UI CCWGG 5 cut(s) 399, 639, 1146, 1276, 1286
Bst4CI ACNGT 5 cut(s) 496, 814, 976, 1018, 1191
Bst6I CTCTTC 1 cut(s) 924
BstAPI GCANNNNNTGC 1 cut(s) 1259
BstBAI YACGTR 1 cut(s) 779
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 4 cut(s) 519, 532, 606, 1077
BstDSI CCRYGG 1 cut(s) 826
BstF5I GGATG 3 cut(s) 255, 352, 419
BstHHI GCGC 1 cut(s) 997
BstKTI GATC 1 cut(s) 154
BstMBI GATC 1 cut(s) 151
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 5 cut(s) 294, 335, 434, 1259, 1268
BstNI CCWGG 5 cut(s) 399, 639, 1146, 1276, 1286
BstNSI RCATGY 3 cut(s) 131, 575, 599
BstSCI CCNGG 5 cut(s) 397, 637, 1144, 1274, 1284
BstSLI GKGCMC 1 cut(s) 1076
BstV1I GCAGC 7 cut(s) 439, 589, 640, 688, 724, 1009, 1237
BstV2I GAAGAC 2 cut(s) 1122, 1236
BstXI CCANNNNNNTGG 1 cut(s) 833
BstZ17I GTATAC 1 cut(s) 461
BsuRI GGCC 5 cut(s) 297, 629, 642, 1074, 1271
BtgI CCRYGG 1 cut(s) 826
BtrI CACGTC 1 cut(s) 1234
BtsCI GGATG 3 cut(s) 255, 352, 419
BtsI GCAGTG 2 cut(s) 53, 570
BtsIMutI CAGTG 7 cut(s) 53, 570, 583, 630, 958, 1100, 1265
Cac8I GCNNGC 1 cut(s) 99
CaiI CAGNNNCTG 1 cut(s) 860
CciI TCATGA 1 cut(s) 1357
CfoI GCGC 1 cut(s) 997
Cfr10I RCCGGY 2 cut(s) 246, 284
Cfr13I GGNCC 4 cut(s) 314, 641, 1072, 1073
Csp6I GTAC 4 cut(s) 876, 1042, 1150, 1279
CviQI GTAC 4 cut(s) 876, 1042, 1150, 1279
DdeI CTNAG 4 cut(s) 519, 532, 606, 1077
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
DriI GACNNNNNGTC 1 cut(s) 312
EaeI YGGCCR 1 cut(s) 1269
Eam1104I CTCTTC 1 cut(s) 924
Eam1105I GACNNNNNGTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 924
EciI GGCGGA 1 cut(s) 1127
Eco130I CCWWGG 1 cut(s) 792
Eco24I GRGCYC 2 cut(s) 86, 1076
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 449
Eco72I CACGTG 1 cut(s) 779
EcoO109I RGGNCCY 1 cut(s) 1073
EcoRII CCWGG 5 cut(s) 397, 637, 1144, 1274, 1284
EcoT14I CCWWGG 1 cut(s) 792
EcoT22I ATGCAT 3 cut(s) 411, 1206, 1264
EcoT38I GRGCYC 2 cut(s) 86, 1076
ErhI CCWWGG 1 cut(s) 792
FalI AAGNNNNNCTT 2 cut(s) 1221, 1253
FaqI GGGAC 2 cut(s) 319, 505
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 8 cut(s) 453, 603, 654, 702, 738, 998, 1251, 1272
FokI GGATG 3 cut(s) 242, 359, 426
FriOI GRGCYC 2 cut(s) 86, 1076
Fsp4HI GCNGC 8 cut(s) 453, 603, 654, 702, 738, 998, 1251, 1272
FspBI CTAG 1 cut(s) 942
FspI TGCGCA 1 cut(s) 996
GlaI GCGC 1 cut(s) 996
GluI GCNGC 8 cut(s) 453, 603, 654, 702, 738, 998, 1251, 1272
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 616
HaeIII GGCC 5 cut(s) 297, 629, 642, 1074, 1271
HapII CCGG 3 cut(s) 247, 285, 1048
HhaI GCGC 1 cut(s) 997
Hin6I GCGC 1 cut(s) 995
HinP1I GCGC 1 cut(s) 995
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 4 cut(s) 797, 857, 924, 1178
HpaII CCGG 3 cut(s) 247, 285, 1048
HphI GGTGA 2 cut(s) 172, 1200
Hpy166II GTNNAC 4 cut(s) 317, 419, 461, 1225
Hpy188I TCNGA 6 cut(s) 170, 233, 335, 960, 1080, 1220
Hpy188III TCNNGA 8 cut(s) 122, 467, 534, 633, 854, 1048, 1133, 1358
Hpy8I GTNNAC 4 cut(s) 317, 419, 461, 1225
HpyAV CCTTC 4 cut(s) 165, 518, 551, 682
HpyCH4III ACNGT 5 cut(s) 496, 814, 976, 1018, 1191
HpyCH4IV ACGT 2 cut(s) 778, 1233
HpyF10VI GCNNNNNNNGC 5 cut(s) 294, 335, 434, 1259, 1268
HpyF3I CTNAG 4 cut(s) 519, 532, 606, 1077
HpySE526I ACGT 2 cut(s) 778, 1233
HspAI GCGC 1 cut(s) 995
Kpn2I TCCGGA 1 cut(s) 1047
KpnI GGTACC 1 cut(s) 1282
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 6 cut(s) 94, 379, 449, 536, 568, 1247
Lsp1109I GCAGC 7 cut(s) 439, 589, 640, 688, 724, 1009, 1237
LweI GCATC 5 cut(s) 264, 337, 396, 415, 1271
MaeI CTAG 1 cut(s) 942
MaeII ACGT 2 cut(s) 778, 1233
MaeIII GTNAC 3 cut(s) 591, 808, 862
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 8 cut(s) 69, 415, 491, 941, 1127, 1147, 1183, 1241
MfeI CAATTG 1 cut(s) 867
MhlI GDGCHC 4 cut(s) 86, 384, 612, 1076
MluCI AATT 8 cut(s) 209, 393, 482, 705, 821, 867, 990, 1140
MlyI GAGTC 1 cut(s) 1172
MmeI TCCRAC 3 cut(s) 535, 796, 934
Mph1103I ATGCAT 3 cut(s) 411, 1206, 1264
MroI TCCGGA 1 cut(s) 1047
MroXI GAANNNNTTC 1 cut(s) 1140
MseI TTAA 2 cut(s) 843, 915
MslI CAYNNNNRTG 4 cut(s) 414, 576, 831, 1340
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 3 cut(s) 247, 285, 1048
MspR9I CCNGG 5 cut(s) 399, 639, 1146, 1276, 1286
MunI CAATTG 1 cut(s) 867
Mva1269I GAATGC 2 cut(s) 1204, 1253
MvaI CCWGG 5 cut(s) 399, 639, 1146, 1276, 1286
MwoI GCNNNNNNNGC 5 cut(s) 294, 335, 434, 1259, 1268
NdeII GATC 1 cut(s) 151
NlaIV GGNNCC 2 cut(s) 1074, 1280
NmuCI GTSAC 1 cut(s) 808
NsbI TGCGCA 1 cut(s) 996
NsiI ATGCAT 3 cut(s) 411, 1206, 1264
NspI RCATGY 3 cut(s) 131, 575, 599
OliI CACNNNNGTG 1 cut(s) 831
PagI TCATGA 1 cut(s) 1357
PciI ACATGT 1 cut(s) 595
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 2 cut(s) 1204, 1253
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 3 cut(s) 797, 857, 924
PfoI TCCNGGA 1 cut(s) 1284
PkrI GCNGC 8 cut(s) 454, 604, 655, 703, 739, 999, 1252, 1273
Ple19I CGATCG 1 cut(s) 154
PleI GAGTC 1 cut(s) 1172
PmaCI CACGTG 1 cut(s) 779
PmlI CACGTG 1 cut(s) 779
PpsI GAGTC 1 cut(s) 1172
Ppu21I YACGTR 1 cut(s) 779
PscI ACATGT 1 cut(s) 595
Psp6I CCWGG 5 cut(s) 397, 637, 1144, 1274, 1284
PspCI CACGTG 1 cut(s) 779
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 5 cut(s) 397, 637, 1144, 1274, 1284
PspN4I GGNNCC 2 cut(s) 1074, 1280
PspOMI GGGCCC 1 cut(s) 1072
PspPI GGNCC 4 cut(s) 314, 641, 1072, 1073
PstNI CAGNNNCTG 1 cut(s) 860
PvuI CGATCG 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 4 cut(s) 877, 1043, 1151, 1280
RsaNI GTAC 4 cut(s) 876, 1042, 1150, 1279
RseI CAYNNNNRTG 4 cut(s) 414, 576, 831, 1340
SaqAI TTAA 2 cut(s) 843, 915
SatI GCNGC 8 cut(s) 453, 603, 654, 702, 738, 998, 1251, 1272
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 4 cut(s) 314, 641, 1072, 1073
ScaI AGTACT 1 cut(s) 1043
SchI GAGTC 1 cut(s) 1172
ScrFI CCNGG 5 cut(s) 399, 639, 1146, 1276, 1286
SduI GDGCHC 4 cut(s) 86, 384, 612, 1076
SfaNI GCATC 5 cut(s) 264, 337, 396, 415, 1271
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 4 cut(s) 414, 576, 831, 1340
SmlI CTYRAG 1 cut(s) 1182
SmoI CTYRAG 1 cut(s) 1182
Sse9I AATT 8 cut(s) 209, 393, 482, 705, 821, 867, 990, 1140
SsiI CCGC 2 cut(s) 1112, 1272
SspMI CTAG 1 cut(s) 942
StyD4I CCNGG 5 cut(s) 397, 637, 1144, 1274, 1284
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 5 cut(s) 496, 814, 976, 1018, 1191
TaiI ACGT 2 cut(s) 781, 1236
TaqI TCGA 4 cut(s) 80, 150, 927, 1062
TasI AATT 8 cut(s) 209, 393, 482, 705, 821, 867, 990, 1140
TatI WGTACW 1 cut(s) 1041
TauI GCSGC 1 cut(s) 1274
TfiI GAWTC 3 cut(s) 797, 857, 924
Tru1I TTAA 2 cut(s) 843, 915
Tru9I TTAA 2 cut(s) 843, 915
TscAI CASTG 7 cut(s) 60, 570, 583, 630, 958, 1107, 1272
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 7 cut(s) 452, 602, 653, 701, 737, 997, 1250
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 7 cut(s) 22, 198, 570, 1002, 1184, 1346, 1358
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 7 cut(s) 60, 570, 583, 630, 958, 1107, 1272
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 3 cut(s) 209, 393, 821
XceI RCATGY 3 cut(s) 131, 575, 599
XcmI CCANNNNNNNNNTGG 2 cut(s) 622, 1282
XmiI GTMKAC 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 1 cut(s) 942
ZrmI AGTACT 1 cut(s) 1043
Zsp2I ATGCAT 3 cut(s) 411, 1206, 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.