Rh2DG121500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000448
Physical Location & Seq
Forward (+)
10132100 .. 10136153
4054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG121500.1

Sequence Viewer

Length: 1074 bp
ATGCAGATGATCAGGTGGCTCCTAAACCTGCAGCAATTTTCATATTTGGAGACTCGACTGTGGATGTTGGCATGCACCAACCAATGGTTGAACAATAGTTATTCGAGAGCTGATTTTTCTCCCAATGGAGTTGATTTTCCTCATTCTGTTCCGACGGGGAGGTTTAGTAATGGCCTTAACAGCGCTGACCAAGTTGTAAGACTATTTAGGTACAAGAGAAGTCCGCAACCATTCTTGTATTCTCTGAACCATCAATCCACTTTCAAAAGAGATATACTGCAGGGAGTTAATTTTGCATCCGGAGGATCCGGCATATTCAACCAAACAGGAAAAAAGGACTATGGAGAAGTTGTGCCACTGGGAGATCAGGTCAAACAATTTGCGACGGTTCGTGGAAATATCACAGAACTAATTGGTCTTGCGGCAACTGAGATAATGTTTTCCAAGTCTTTGTTCGTTATTAGTATAGGAAGCAATGACCTGTTCGAGTTAGTGAAATGGCATCCAAATATCACTTCCTTGGCTAAGGATGATGATGCGGGAAGCTACTTGGAAACTCTTAAACTCAACTACAGTAACCATCTGAAGGACTTATACAATCTGGGAGCTAGAAAATTCGGGATTATAAGTGTTCCTCCAATTGGATGCTGTCCTGCTGCACGTGTTGGACCAAAATCGAATGACTCCAGTGTTTGTGTAGAGGAGCTAAACAATCTTGCCTCAACATTTCTTGCAAAAACTGAGGATCTCCTCCAAAAATTGAGCTCAGAGTTGAAAGGACTCGTGTACTCGCTTGGAAATGCTTATGAAATGACTATGAGTATACTTGAAGACCCATTGGCGTTTGGCTTCAAGGACACTCAATCAGCCTGCTGTGGGTCAGGAAAGTTAAATGGAGTGTTTCCTTGCTTCTTCTTACTCGGTCCCAACCTTTGTCCGAATCGGCGGGAGGTTTTGTTCTGGGATTTGTATCATCCTACGGAATATGCTTCCGAGCTAGCAGCACTAACCCTTTATGGTGGAGGAACAAGATACGTCACACCTATAAACTTTAGTCAGTTGGGGTGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

39.75

Weight (kDa)

6.94

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 51 - 335 4.2e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 626
Acc36I ACCTGC 1 cut(s) 36
AccB7I CCANNNNNTGG 1 cut(s) 84
AccI GTMKAC 1 cut(s) 823
AccIII TCCGGA 1 cut(s) 299
AciI CCGC 4 cut(s) 224, 422, 539, 946
AclWI GGATC 3 cut(s) 300, 313, 753
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 1 cut(s) 605
AcvI CACGTG 1 cut(s) 662
AfaI GTAC 2 cut(s) 212, 788
AfeI AGCGCT 1 cut(s) 184
AfiI CCNNNNNNNGG 4 cut(s) 84, 586, 641, 876
AflIII ACRYGT 1 cut(s) 661
AgsI TTSAA 6 cut(s) 91, 265, 319, 775, 830, 853
AjuI GAANNNNNNNTTGG 4 cut(s) 437, 469, 499, 531
AloI GAACNNNNNNTCC 4 cut(s) 239, 271, 941, 973
AluBI AGCT 6 cut(s) 110, 546, 608, 706, 765, 997
AluI AGCT 6 cut(s) 110, 546, 608, 706, 765, 997
Alw21I GWGCWC 1 cut(s) 767
Alw26I GTCTC 1 cut(s) 44
AlwI GGATC 3 cut(s) 300, 313, 753
Aor13HI TCCGGA 1 cut(s) 299
Aor51HI AGCGCT 1 cut(s) 184
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 3 cut(s) 31, 656, 1001
ApoI RAATTY 1 cut(s) 614
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 2 cut(s) 668, 923
AsuNHI GCTAGC 1 cut(s) 997
AvaII GGWCC 2 cut(s) 668, 923
BamHI GGATCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 767
BarI GAAGNNNNNNTAC 2 cut(s) 578, 610
BauI CACGAG 1 cut(s) 782
BbrPI CACGTG 1 cut(s) 662
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 1 cut(s) 767
BbvI GCAGC 3 cut(s) 43, 643, 1013
BccI CCATC 2 cut(s) 258, 588
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 44
BfaI CTAG 2 cut(s) 609, 998
BfmI CTRYAG 3 cut(s) 29, 278, 571
BfoI RGCGCY 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 36
BisI GCNGC 4 cut(s) 32, 423, 657, 1002
BlsI GCNGC 4 cut(s) 33, 424, 658, 1003
Bme18I GGWCC 2 cut(s) 668, 923
BmgT120I GGNCC 2 cut(s) 668, 923
BmiI GGNNCC 3 cut(s) 20, 307, 925
BmrI ACTGGG 1 cut(s) 368
BmsI GCATC 4 cut(s) 305, 511, 526, 635
BmtI GCTAGC 1 cut(s) 1001
BmuI ACTGGG 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 1 cut(s) 670
Bpu10I CCTNAGC 1 cut(s) 525
BsaAI YACGTR 1 cut(s) 662
BsaBI GATNNNNATC 1 cut(s) 969
BsaJI CCNNGG 1 cut(s) 519
BsaWI WCCGGW 1 cut(s) 299
BsaXI ACNNNNNCTCC 8 cut(s) 151, 181, 336, 354, 366, 384, 941, 971
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 586, 641, 876
Bse1I ACTGG 2 cut(s) 363, 687
Bse3DI GCAATG 1 cut(s) 481
Bse8I GATNNNNATC 1 cut(s) 969
BseAI TCCGGA 1 cut(s) 299
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 6 cut(s) 69, 296, 502, 535, 650, 973
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 4 cut(s) 84, 586, 641, 876
BseMI GCAATG 1 cut(s) 481
BseMII CTCAG 3 cut(s) 420, 732, 780
BseNI ACTGG 2 cut(s) 363, 687
BseRI GAGGAG 2 cut(s) 716, 740
BseXI GCAGC 3 cut(s) 43, 643, 1013
BsgI GTGCAG 1 cut(s) 642
BshFI GGCC 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 767
BsiSI CCGG 2 cut(s) 300, 309
BslFI GGGAC 1 cut(s) 909
BslI CCNNNNNNNGG 4 cut(s) 84, 586, 641, 876
BsmAI GTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 909
BsnI GGCC 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 767
Bsp13I TCCGGA 1 cut(s) 299
Bsp143I GATC 4 cut(s) 9, 305, 364, 745
BspACI CCGC 4 cut(s) 224, 422, 539, 946
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 3 cut(s) 421, 733, 779
BspEI TCCGGA 1 cut(s) 299
BspLI GGNNCC 3 cut(s) 20, 307, 925
BspMAI CTGCAG 2 cut(s) 33, 282
BspMI ACCTGC 1 cut(s) 36
BspOI GCTAGC 1 cut(s) 1001
BspPI GGATC 3 cut(s) 300, 313, 753
BsrDI GCAATG 1 cut(s) 481
BsrI ACTGG 2 cut(s) 363, 687
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 4 cut(s) 9, 305, 364, 745
BssNAI GTATAC 1 cut(s) 824
BssSI CACGAG 1 cut(s) 782
BssT1I CCWWGG 1 cut(s) 519
Bst1107I GTATAC 1 cut(s) 824
Bst2BI CACGAG 1 cut(s) 782
Bst4CI ACNGT 3 cut(s) 60, 388, 575
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 3 cut(s) 73, 871, 999
BstDEI CTNAG 4 cut(s) 429, 525, 741, 766
BstF5I GGATG 6 cut(s) 69, 296, 502, 535, 650, 973
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 4 cut(s) 12, 308, 367, 748
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 4 cut(s) 9, 305, 364, 745
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstNSI RCATGY 1 cut(s) 75
BstSFI CTRYAG 3 cut(s) 29, 278, 571
BstV1I GCAGC 3 cut(s) 43, 643, 1013
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 2 cut(s) 305, 745
BstYI RGATCY 2 cut(s) 305, 745
BstZ17I GTATAC 1 cut(s) 824
BsuRI GGCC 1 cut(s) 174
BtsCI GGATG 6 cut(s) 69, 296, 502, 535, 650, 973
BtsIMutI CAGTG 2 cut(s) 356, 694
BveI ACCTGC 1 cut(s) 36
Cac8I GCNNGC 3 cut(s) 73, 871, 999
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 668, 923
Csp6I GTAC 2 cut(s) 211, 787
CviAII CATG 1 cut(s) 72
CviQI GTAC 2 cut(s) 211, 787
DdeI CTNAG 4 cut(s) 429, 525, 741, 766
DpnI GATC 4 cut(s) 11, 307, 366, 747
DpnII GATC 4 cut(s) 9, 305, 364, 745
Ecl136II GAGCTC 1 cut(s) 765
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 767
Eco47I GGWCC 2 cut(s) 668, 923
Eco47III AGCGCT 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 765
Eco57I CTGAAG 1 cut(s) 605
Eco72I CACGTG 1 cut(s) 662
EcoICRI GAGCTC 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 767
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 1 cut(s) 75
FaqI GGGAC 1 cut(s) 909
FatI CATG 1 cut(s) 71
FauI CCCGC 2 cut(s) 532, 939
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 1 cut(s) 823
Fnu4HI GCNGC 4 cut(s) 32, 423, 657, 1002
FokI GGATG 6 cut(s) 76, 283, 489, 542, 657, 960
FriOI GRGCYC 1 cut(s) 767
Fsp4HI GCNGC 4 cut(s) 32, 423, 657, 1002
FspBI CTAG 2 cut(s) 609, 998
GlaI GCGC 1 cut(s) 184
GluI GCNGC 4 cut(s) 32, 423, 657, 1002
GsuI CTGGAG 1 cut(s) 670
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 1 cut(s) 174
HapII CCGG 2 cut(s) 300, 309
HhaI GCGC 1 cut(s) 185
Hin1II CATG 1 cut(s) 75
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HinfI GANTC 4 cut(s) 52, 683, 780, 940
HpaII CCGG 2 cut(s) 300, 309
Hpy166II GTNNAC 2 cut(s) 787, 824
Hpy188I TCNGA 6 cut(s) 153, 246, 585, 769, 939, 994
Hpy188III TCNNGA 4 cut(s) 105, 300, 619, 882
Hpy8I GTNNAC 2 cut(s) 787, 824
Hpy99I CGWCG 2 cut(s) 157, 388
HpyAV CCTTC 1 cut(s) 580
HpyCH4III ACNGT 3 cut(s) 60, 388, 575
HpyCH4IV ACGT 2 cut(s) 661, 1035
HpyCH4V TGCA 7 cut(s) 4, 31, 75, 280, 296, 659, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 4 cut(s) 429, 525, 741, 766
HpySE526I ACGT 2 cut(s) 661, 1035
Hsp92II CATG 1 cut(s) 75
HspAI GCGC 1 cut(s) 183
Kpn2I TCCGGA 1 cut(s) 299
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 4 cut(s) 9, 305, 364, 745
LmnI GCTCC 3 cut(s) 24, 605, 703
Lsp1109I GCAGC 3 cut(s) 43, 643, 1013
LweI GCATC 4 cut(s) 305, 511, 526, 635
MaeI CTAG 2 cut(s) 609, 998
MaeII ACGT 2 cut(s) 661, 1035
MaeIII GTNAC 2 cut(s) 575, 1036
MalI GATC 4 cut(s) 11, 307, 366, 747
MboI GATC 4 cut(s) 9, 305, 364, 745
MboII GAAGA 2 cut(s) 842, 904
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 2 cut(s) 305, 745
MhlI GDGCHC 1 cut(s) 767
MluCI AATT 7 cut(s) 35, 289, 377, 411, 614, 639, 758
MlyI GAGTC 3 cut(s) 46, 677, 774
MmeI TCCRAC 2 cut(s) 176, 646
MroI TCCGGA 1 cut(s) 299
MseI TTAA 4 cut(s) 177, 288, 561, 890
MspI CCGG 2 cut(s) 300, 309
MunI CAATTG 1 cut(s) 639
MwoI GCNNNNNNNGC 1 cut(s) 180
NdeII GATC 4 cut(s) 9, 305, 364, 745
NheI GCTAGC 1 cut(s) 997
NlaIII CATG 1 cut(s) 75
NlaIV GGNNCC 3 cut(s) 20, 307, 925
NmuCI GTSAC 1 cut(s) 1036
NspI RCATGY 1 cut(s) 75
PaeI GCATGC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 940
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 4 cut(s) 33, 424, 658, 1003
PleI GAGTC 3 cut(s) 46, 677, 774
PmaCI CACGTG 1 cut(s) 662
PmlI CACGTG 1 cut(s) 662
PpsI GAGTC 3 cut(s) 46, 677, 774
Ppu21I YACGTR 1 cut(s) 662
PsiI TTATAA 1 cut(s) 626
Psp124BI GAGCTC 1 cut(s) 767
PspCI CACGTG 1 cut(s) 662
PspN4I GGNNCC 3 cut(s) 20, 307, 925
PspPI GGNCC 2 cut(s) 668, 923
PstI CTGCAG 2 cut(s) 33, 282
PsuI RGATCY 2 cut(s) 305, 745
RsaI GTAC 2 cut(s) 212, 788
RsaNI GTAC 2 cut(s) 211, 787
SacI GAGCTC 1 cut(s) 767
SaqAI TTAA 4 cut(s) 177, 288, 561, 890
SatI GCNGC 4 cut(s) 32, 423, 657, 1002
Sau3AI GATC 4 cut(s) 9, 305, 364, 745
Sau96I GGNCC 2 cut(s) 668, 923
SchI GAGTC 3 cut(s) 46, 677, 774
SduI GDGCHC 1 cut(s) 767
SfaNI GCATC 4 cut(s) 305, 511, 526, 635
SfcI CTRYAG 3 cut(s) 29, 278, 571
SinI GGWCC 2 cut(s) 668, 923
SphI GCATGC 1 cut(s) 75
Sse9I AATT 7 cut(s) 35, 289, 377, 411, 614, 639, 758
SsiI CCGC 4 cut(s) 224, 422, 539, 946
SspMI CTAG 2 cut(s) 609, 998
SstI GAGCTC 1 cut(s) 767
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 3 cut(s) 60, 388, 575
TaiI ACGT 2 cut(s) 664, 1038
TaqI TCGA 4 cut(s) 55, 104, 486, 677
TaqII GACCGA 1 cut(s) 911
TasI AATT 7 cut(s) 35, 289, 377, 411, 614, 639, 758
TatI WGTACW 1 cut(s) 786
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 1 cut(s) 940
Tru1I TTAA 4 cut(s) 177, 288, 561, 890
Tru9I TTAA 4 cut(s) 177, 288, 561, 890
TscAI CASTG 2 cut(s) 363, 694
TseFI GTSAC 1 cut(s) 1036
TseI GCWGC 3 cut(s) 31, 656, 1001
Tsp45I GTSAC 1 cut(s) 1036
TspDTI ATGAA 2 cut(s) 30, 822
TspGWI ACGGA 1 cut(s) 995
TspRI CASTG 2 cut(s) 363, 694
Van91I CCANNNNNTGG 1 cut(s) 84
VpaK11BI GGWCC 2 cut(s) 668, 923
XapI RAATTY 1 cut(s) 614
XceI RCATGY 1 cut(s) 75
XmiI GTMKAC 1 cut(s) 823
XspI CTAG 2 cut(s) 609, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.