Rh2CG121200

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000448
Physical Location & Seq
Forward (+)
10765740 .. 10766942
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG121200.1

Sequence Viewer

Length: 672 bp
ATGCTAGGTTTCCATGCATCCTCTTCACAGCAAATTGTGCCTGCAATTTACATATTTGGAGATTCTCGGGGAGATGTTGGAACCAATAATTACTTGCCTGATTGTACTGCAAGGGCTGATATCCTCTATAATGGGATTGACTACCCTCACTCTAAACCAACAGGAAGATTTAGCAACGGTTACAACGTCGTAGATTACATCGCCCAGTTTCTGGGTTACACGAAGAGCCCGCCACCTTTTCTCTCTCTTTTAGACGAGAAGAAGCAATTTCCTAACAGGAAGGTTCCAAATAAGGGAGTTAACTTTGCGTCAGGAGGATCAGGACTTCTGGATGATACTGGTAAACTACAGTGGGGAAATGTCATTTCCTTGGGGGAACAAGTGCAGCAATTCCAAACTTTACGGAACAAAATCTCAGAATTATTGGGTGCTGGTGGATTGGCTAATATTTCTCAGTCTCTCTTTGTCATCAGCGTTGGAAGCAACGACATCTTTGAATTATTCACTGCTAATAACCTGACTAAAAGAACCGAGCAAGACTACTTGAGCATACTCATCTCCTCTTACGAAATTCATTTAAGGAACTTATTTATGCTCGGAGCAAGGAAGTTTGGGATTATCGATCAGCCTCTCACAAATTGGGTGCCGCCCTGCCCAGCGAAAGCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

223

Amino Acids

24.55

Weight (kDa)

7.74

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 16 - 207 3.5e-15 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 643
AccB7I CCANNNNNTGG 1 cut(s) 211
AciI CCGC 2 cut(s) 230, 647
AclWI GGATC 1 cut(s) 325
AcsI RAATTY 1 cut(s) 570
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 2 cut(s) 211, 293
AgsI TTSAA 1 cut(s) 497
AluBI AGCT 1 cut(s) 665
AluI AGCT 1 cut(s) 665
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 1 cut(s) 325
AlwNI CAGNNNCTG 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 1 cut(s) 570
AvaI CYCGRG 1 cut(s) 66
BanI GGYRCC 1 cut(s) 643
BanII GRGCYC 1 cut(s) 230
BbvI GCAGC 1 cut(s) 397
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 1 cut(s) 5
BfmI CTRYAG 1 cut(s) 347
BisI GCNGC 2 cut(s) 386, 647
BlsI GCNGC 2 cut(s) 387, 648
BmeT110I CYCGRG 1 cut(s) 66
BmiI GGNNCC 3 cut(s) 82, 285, 645
BmrI ACTGGG 1 cut(s) 199
BmsI GCATC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 565
Bsa29I ATCGAT 1 cut(s) 621
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 293
Bse1I ACTGG 2 cut(s) 205, 343
BseCI ATCGAT 1 cut(s) 621
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 17, 337
BseLI CCNNNNNNNGG 2 cut(s) 211, 293
BseMII CTCAG 2 cut(s) 429, 467
BseNI ACTGG 2 cut(s) 205, 343
BseRI GAGGAG 1 cut(s) 550
BseXI GCAGC 1 cut(s) 397
BseYI CCCAGC 1 cut(s) 655
BsgI GTGCAG 1 cut(s) 404
BshNI GGYRCC 1 cut(s) 643
BshVI ATCGAT 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 66
BslI CCNNNNNNNGG 2 cut(s) 211, 293
BsmAI GTCTC 1 cut(s) 462
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 2 cut(s) 317, 622
BspACI CCGC 2 cut(s) 230, 647
BspCNI CTCAG 2 cut(s) 428, 466
BspDI ATCGAT 1 cut(s) 621
BspLI GGNNCC 3 cut(s) 82, 285, 645
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 643
BsrI ACTGG 2 cut(s) 205, 343
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 2 cut(s) 317, 622
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 2 cut(s) 179, 351
Bst6I CTCTTC 2 cut(s) 28, 218
BstAPI GCANNNNNTGC 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 42, 230
BstDEI CTNAG 3 cut(s) 415, 453, 666
BstF5I GGATG 2 cut(s) 17, 337
BstKTI GATC 2 cut(s) 320, 625
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 2 cut(s) 317, 622
BstMWI GCNNNNNNNGC 2 cut(s) 37, 480
BstSFI CTRYAG 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 397
Bsu15I ATCGAT 1 cut(s) 621
BsuTUI ATCGAT 1 cut(s) 621
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 17, 337
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 356, 504
Cac8I GCNNGC 2 cut(s) 42, 230
CaiI CAGNNNCTG 1 cut(s) 211
ClaI ATCGAT 1 cut(s) 621
CseI GACGC 1 cut(s) 297
Csp6I GTAC 1 cut(s) 105
CviAII CATG 1 cut(s) 14
CviJI RGCY 5 cut(s) 116, 228, 443, 628, 665
CviKI_1 RGCY 5 cut(s) 116, 228, 443, 628, 665
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 3 cut(s) 415, 453, 666
DpnI GATC 2 cut(s) 319, 624
DpnII GATC 2 cut(s) 317, 622
Eam1104I CTCTTC 2 cut(s) 28, 218
EarI CTCTTC 2 cut(s) 28, 218
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 66
EcoRV GATATC 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 19
EcoT38I GRGCYC 1 cut(s) 230
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 1 cut(s) 17
FaiI YATR 5 cut(s) 15, 53, 129, 551, 593
FatI CATG 1 cut(s) 13
FauI CCCGC 1 cut(s) 237
Fnu4HI GCNGC 2 cut(s) 386, 647
FokI GGATG 2 cut(s) 4, 344
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 2 cut(s) 386, 647
FspBI CTAG 1 cut(s) 5
GluI GCNGC 2 cut(s) 386, 647
GsaI CCCAGC 1 cut(s) 659
HgaI GACGC 1 cut(s) 297
Hin1II CATG 1 cut(s) 17
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 1 cut(s) 62
HpaI GTTAAC 1 cut(s) 301
Hpy166II GTNNAC 2 cut(s) 301, 344
Hpy188I TCNGA 2 cut(s) 418, 599
Hpy188III TCNNGA 3 cut(s) 312, 321, 329
Hpy8I GTNNAC 2 cut(s) 301, 344
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 2 cut(s) 179, 351
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 4 cut(s) 17, 44, 110, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 480
HpyF3I CTNAG 3 cut(s) 415, 453, 666
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 1 cut(s) 17
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 2 cut(s) 317, 622
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 1 cut(s) 599
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 1 cut(s) 26
MaeI CTAG 1 cut(s) 5
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 179, 215
MalI GATC 2 cut(s) 319, 624
MboI GATC 2 cut(s) 317, 622
MboII GAAGA 4 cut(s) 15, 177, 235, 271
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 9 cut(s) 33, 45, 88, 266, 389, 419, 497, 570, 637
MmeI TCCRAC 2 cut(s) 58, 457
MnlI CCTC 6 cut(s) 31, 134, 156, 308, 571, 639
Mph1103I ATGCAT 1 cut(s) 19
MseI TTAA 2 cut(s) 300, 578
MwoI GCNNNNNNNGC 2 cut(s) 37, 480
NdeII GATC 2 cut(s) 317, 622
NlaIII CATG 1 cut(s) 17
NlaIV GGNNCC 3 cut(s) 82, 285, 645
NsiI ATGCAT 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PfeI GAWTC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 2 cut(s) 387, 648
PspFI CCCAGC 1 cut(s) 655
PspN4I GGNNCC 3 cut(s) 82, 285, 645
PstNI CAGNNNCTG 1 cut(s) 211
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 2 cut(s) 300, 578
SatI GCNGC 2 cut(s) 386, 647
Sau3AI GATC 2 cut(s) 317, 622
SduI GDGCHC 1 cut(s) 230
SetI ASST 6 cut(s) 10, 189, 238, 285, 519, 667
SfaNI GCATC 1 cut(s) 26
SfcI CTRYAG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 544
SmoI CTYRAG 1 cut(s) 544
Sse9I AATT 9 cut(s) 33, 45, 88, 266, 389, 419, 497, 570, 637
SsiI CCGC 2 cut(s) 230, 647
SspI AATATT 1 cut(s) 448
SspMI CTAG 1 cut(s) 5
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 2 cut(s) 179, 351
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 621
TasI AATT 9 cut(s) 33, 45, 88, 266, 389, 419, 497, 570, 637
TatI WGTACW 1 cut(s) 104
TauI GCSGC 1 cut(s) 649
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 2 cut(s) 300, 578
Tru9I TTAA 2 cut(s) 300, 578
TscAI CASTG 2 cut(s) 356, 511
TseI GCWGC 1 cut(s) 385
TspDTI ATGAA 1 cut(s) 563
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 2 cut(s) 356, 511
Van91I CCANNNNNTGG 1 cut(s) 211
XapI RAATTY 1 cut(s) 570
XspI CTAG 1 cut(s) 5
Zsp2I ATGCAT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.