Rorug02G0069800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000246
Physical Location & Seq
Forward (+)
5424094 .. 5429283
5190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0069800.1

Sequence Viewer

Length: 2256 bp
ATGGGGTGTGAGAGCAACCTGAAGACATGGGTGTCTGATAAGTTAATAGCATTGCTCGGATACTCTAATCCGGTAGTTGTTCAGTATGTTATTGGATTAACTAAAAAAACAAACTCCCAGGTTGATTTGGTGGACAAGCTTGTCGAATTTGGATTTGCGTCATCGACTGAAACAGGGGCATTTGCTGCACATATCTTTGCAAGAGTGTGTGGTGAAACATCTGGTTTGAAGCAATGCGGGAAACAAGAGAGGGGAGGTGCAATGCCGGTGAGGATGATGCAGCGGACCCATGTTCTGTTGGATTCAGATGATGATGGTGATGCGGAGAGATGTTCTGTTGAGGTTGTTTCTCATTCTGTGTCTGTAACAGTGGATTTCAATAACAAAAGATTCAGGAAGAGGGCATTGGGTCAGAAAGATGGAGATGATGATGAGGTTATTGTAGAACGTGTTAAAAGGCGGAACTTGCCAGATAAAGATGATCATGAGGATGGTTCAGAGTCAGAAGAAGAGAGATTGCGTGATCATAGAGAAAGGGAGAAATTGGAGCAAAGTATAAGGAAGAGGGACGCAGCAGCCACGCGAAAACTGACAAAGAAAAACTTAAGACGAAAAGGGGAAGAGGAGGCTGTTCGAAAAAACAGTGGTGATTTTGAAGGTTTAAGAAGAGCTTCAAGACAAGAATATCTAAAGGAAAGAGAGAGAAAGAAAATGGATGAAATGATGGATGATGTAGAAGATGAACATTACTTATTTGAAGGTGTGAAGCTCACTGAAGCAGAATTCCGGGAATTAAGTTACAAGAAGCAAATATTGGAGCTTATGAGAAAACCATTAGGTCTAGATGAGGCTGAAAATGTCACCGAGTACAGGATGCCAGATGCCTATGATGATGAGGAGCGCGGTGTTACTCAACAGAAGAGATTTTCTGTGGCTTTGCAGCGCTACAAGGACGACAGTTCTGGGGATAAAATGAAATCGTTTGGACAGCAGAAATCTTGGGAAGATCAGCAACTTGGAAAAGCAACATTGGAATTTGGTTCAAAAAACAAAAAAAGGGTATCTGATGAGTATGATTTTGTATATGAAGACCAGATTGAGTTCGTCAGGGGGTTGGTGATCGAGGATGATAAATATGACAAGTTTGAGGATGATAATCAACTGCAGTCCACAAAATTACTTGAGTCAAGGGAAAAAACCTTGAAGATGTTCCAGGAGGAGAGGAAAACTTTACCCATCTTCTCCTTTCATGATGAGTTGCTCCAAGCTGTTAAAGATCATCAGGTTCTTGTTATTGTTGGCGAAACTGGATCTGGCAAAACTACACAGATTCCCCAGTATTTGCATGAGGCAGGATATACAAAGCATGGAAAGATTGGGTGCACACAGCCACGGCGAGTTGCTGCTATGAGTGTTGCTGCCAGGGTTTCTCAAGAAATGGGTGTCAAGCTTGGACATGAGGTAGGTTATTCTATTCGTTTTGAGGATTGCACGTCTGAAAAGACCGTTTTGAAATATATGACTGATGGAATGTTGTTGCGTGAATTTCTTGCTGACCCAGATTTGGCAAGCTATAGTGTGCTGATGGTGGATGAGGCCCATGAGAGAACACTCTCTACTGATATTCTATTTGCATTAGTAAAGGATATTGCTCGATTTCGACCCGGTTTTAAGCTGCTTATCTCGAGTGCAACACTTGATGCTGTGAAGTTCAGTAATTATTTTGATTGTGCCCCAATTTTCAACATTCCAGGGAGGCGGTTTCCTGTCGATATATACCACACAAAAGCACCAGAAGCTGATTACTTAGATGCTGCAATTGCTGCTGCAATTCAAATCCATGTCAGGGAACCACCTGGAGACATATTGGTCTTCCTCACGGGTCAAGAAGATATAGAAACAGCAAGCGAAACATTAAAGTACAGGACAAGTGGTCTCGGGAGAAAGATATCAGAGCTGATTATCTGTCCCATATATGCAAACCTGCCTACTGAGCAGCAAGCAAAAATTTTTGAGCCCACACCTGAAGGGGCTAGAAAGGTTGTCCTTGCCACAAACATAGCTGAAACTTCTCTGACTATTGATGGGATCAAATATGTCATCGACTGTGGCTATTGCAAGATGAAGTCCTATAATCCAAGGACTGGGATGGAGTCATTGCAAGTCACTCCCATCTCAAAGGCATCAGCAAGGCAGAGGGCAGGTCGATCTGGTCGAACAGGCCCTGGCTTAAGAGCCTTGGTATCCATGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

751

Amino Acids

85.19

Weight (kDa)

5.88

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_22 PF13401 426 - 553 1.8e-06 AAA domain
Helicase_C PF00271 610 - 741 3.7e-13 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44080 AT1G57906 AT1G65735 AT1G65740 AT1G65760 AT1G65770 AT2G17690 AT2G26160 AT3G25750 AT3G25750 AT4G35733
fragaria_vesca FvH4_1g10810 FvH4_1g10830 FvH4_1g10840 FvH4_1g10840 FvH4_1g10840 FvH4_1g10860 FvH4_1g10880
malus_domestica MD02G1122000.v1.1 MD15G1235700.v1.1 MD15G1235900.v1.1
prunus_persica Prupe.7G177000_v2.0.a1 Prupe.7G177100_v2.0.a1 Prupe.7G177200_v2.0.a1 Prupe.7G177300_v2.0.a1 Prupe.7G177400_v2.0.a1 Prupe.7G177400_v2.0.a1
pyrus_communis pycom02g09500 pycom15g20990 pycom15g21000
rosa_chinensis RchiOBHm_Chr1g0325601 RchiOBHm_Chr2g0098071 RchiOBHm_Chr2g0098081 RchiOBHm_Chr2g0098091 RchiOBHm_Chr2g0098121 RchiOBHm_Chr2g0098131 RchiOBHm_Chr2g0098151 RchiOBHm_Chr2g0134711 RchiOBHm_Chr3g0456141 RchiOBHm_Chr4g0394811 RchiOBHm_Chr4g0416561 RchiOBHm_Chr5g0014321
rosa_laevigata RLG00000001178 RLG00000016753 RLG00000016754 RLG00000016755 RLG00000016756 RLG00000016757 RLG00000016758 RLG00000016760 RLG00000016761 RLG00000016762 RLG00000016763
rosa_multiflora Rmu_co8307469.1_g000001 Rmu_sc0000427.1_g000002 Rmu_sc0002880.1_g000004 Rmu_sc0002880.1_g000007 Rmu_sc0009178.1_g000019 Rmu_sc0013919.1_g000002 Rmu_sc0013919.1_g000003 Rmu_sc0014278.1_g000001 Rmu_sc0031755.1_g000001 Rmu_sc0031755.1_g000002 Rmu_sc0039043.1_g000001 Rmu_sc0039432.1_g000001
rosa_roxburghii Rroxscaffold_2G00144260 Rroxscaffold_2G00144270 Rroxscaffold_2G00144290 Rroxscaffold_2G00144310 Rroxscaffold_2G00144320 Rroxscaffold_2G00144340 Rroxscaffold_3G00238070
rosa_rugosa Rorug02G0069800 Rorug02G0069900 Rorug02G0070000 Rorug02G0070100 Rorug02G0070200 Rorug02G0070500 Rorug02G0070500 Rorug05G0053500 Rorug05G0482400 Rorug07G0244200
rosa_samantha Rh1DG210300 Rh2AG117100 Rh2AG117200 Rh2AG117300 Rh2AG117500 Rh2AG117700 Rh2AG117800 Rh2AG563300 Rh2BG119900 Rh2BG120000 Rh2BG120200 Rh2BG120300 Rh2BG120400 Rh2BG120600 Rh2BG120700 Rh2BG377200 Rh2CG076100 Rh2CG076200 Rh2CG121600 Rh2CG121700 Rh2CG121900 Rh2CG122000 Rh2CG122100 Rh2CG122200 Rh2CG122300 Rh2DG121600 Rh2DG121700 Rh2DG122000 Rh2DG122100 Rh2DG122300 Rh3AG138900 Rh3BG074100 Rh3CG073500 Rh3DG298600 Rh4CG222800 Rh4CG222900 Rh5CG120400 Rh6BG018400 Rh6BG124600 Rh6CG123200 Rh6CG123300 Rh7AG342600 Rh7AG342700 Rh7BG352100 Rh7CG128700 Rh7CG360200 Rh7CG360300
rosa_wichuraiana Rw2G009150 Rw2G009160 Rw2G009170 Rw2G009200 Rw4G016710 Rw5G035380 Rw6G043260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 31, 140, 1868
Acc36I ACCTGC 2 cut(s) 1992, 2192
AccB7I CCANNNNNTGG 1 cut(s) 2144
AccII CGCG 2 cut(s) 583, 903
AciI CCGC 6 cut(s) 237, 283, 323, 460, 903, 1759
AclWI GGATC 2 cut(s) 1318, 2096
AcsI RAATTY 5 cut(s) 146, 782, 1034, 1544, 2007
AcuI CTGAAG 3 cut(s) 41, 795, 2046
AfaI GTAC 2 cut(s) 869, 1922
AfeI AGCGCT 1 cut(s) 944
AfiI CCNNNNNNNGG 4 cut(s) 870, 1564, 1846, 2144
AflII CTTAAG 2 cut(s) 604, 2230
AflIII ACRYGT 1 cut(s) 448
AhdI GACNNNNNGTC 1 cut(s) 1932
AjiI CACGTC 1 cut(s) 1494
AjnI CCWGG 6 cut(s) 117, 1212, 1421, 1750, 1855, 2224
AjuI GAANNNNNNNTTGG 4 cut(s) 389, 421, 797, 829
Alw21I GWGCWC 1 cut(s) 1385
Alw26I GTCTC 2 cut(s) 1854, 1940
Alw44I GTGCAC 1 cut(s) 1381
AlwI GGATC 2 cut(s) 1318, 2096
AlwNI CAGNNNCTG 2 cut(s) 1799, 2225
Ama87I CYCGRG 2 cut(s) 1684, 1937
Aor51HI AGCGCT 1 cut(s) 944
AoxI GGCC 2 cut(s) 1596, 2221
ApaLI GTGCAC 1 cut(s) 1381
ApoI RAATTY 5 cut(s) 146, 782, 1034, 1544, 2007
Asp700I GAANNNNTTC 2 cut(s) 670, 1208
AspLEI GCGC 2 cut(s) 903, 945
AspS9I GGNCC 3 cut(s) 285, 1597, 2222
AsuC2I CCSGG 2 cut(s) 788, 1665
AsuHPI GGTGA 6 cut(s) 224, 280, 329, 659, 853, 1129
AsuII TTCGAA 1 cut(s) 634
AvaI CYCGRG 2 cut(s) 1684, 1937
AvaII GGWCC 1 cut(s) 285
BaeGI GKGCMC 2 cut(s) 1385, 1735
BanII GRGCYC 1 cut(s) 2019
BbsI GAAGAC 3 cut(s) 29, 1095, 1864
Bbv12I GWGCWC 1 cut(s) 1385
BceAI ACGGC 1 cut(s) 1409
BciT130I CCWGG 6 cut(s) 119, 1214, 1423, 1752, 1857, 2226
BciVI GTATCC 2 cut(s) 53, 2254
BclI TGATCA 2 cut(s) 481, 523
BcnI CCSGG 2 cut(s) 788, 1665
BcoDI GTCTC 2 cut(s) 1854, 1940
BfaI CTAG 2 cut(s) 842, 2034
BfmI CTRYAG 2 cut(s) 1163, 1573
BfoI RGCGCY 1 cut(s) 946
BfrI CTTAAG 2 cut(s) 604, 2230
BfuAI ACCTGC 2 cut(s) 1992, 2192
BfuI GTATCC 2 cut(s) 53, 2254
Bme1390I CCNGG 8 cut(s) 119, 788, 1214, 1423, 1665, 1752, 1857, 2226
Bme18I GGWCC 1 cut(s) 285
BmeRI GACNNNNNGTC 1 cut(s) 1932
BmeT110I CYCGRG 2 cut(s) 1684, 1937
BmgBI CACGTC 1 cut(s) 1494
BmgT120I GGNCC 3 cut(s) 285, 1597, 2222
BmiI GGNNCC 2 cut(s) 287, 1851
BmrFI CCNGG 8 cut(s) 119, 788, 1214, 1423, 1665, 1752, 1857, 2226
BmrI ACTGGG 2 cut(s) 1330, 2154
BmsI GCATC 7 cut(s) 267, 310, 864, 871, 1690, 1801, 2192
BmuI ACTGGG 2 cut(s) 1330, 2154
BpiI GAAGAC 3 cut(s) 29, 1095, 1864
BpmI CTGGAG 1 cut(s) 1878
Bpu14I TTCGAA 1 cut(s) 634
BpuEI CTTGAG 2 cut(s) 1202, 1416
BpuMI CCSGG 2 cut(s) 788, 1665
BsaBI GATNNNNATC 1 cut(s) 1155
BsaI GGTCTC 1 cut(s) 1940
BsaJI CCNNGG 7 cut(s) 117, 1391, 1422, 1751, 2138, 2224, 2238
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 4 cut(s) 870, 1564, 1846, 2144
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 3 cut(s) 1312, 1336, 2149
Bse3DI GCAATG 4 cut(s) 50, 239, 267, 2156
Bse8I GATNNNNATC 1 cut(s) 1155
BseBI CCWGG 6 cut(s) 119, 1214, 1423, 1752, 1857, 2226
BseDI CCNNGG 7 cut(s) 117, 1391, 1422, 1751, 2138, 2224, 2238
BseGI GGATG 9 cut(s) 279, 496, 721, 733, 879, 1132, 1156, 1597, 2154
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 4 cut(s) 870, 1564, 1846, 2144
BseMI GCAATG 4 cut(s) 50, 239, 267, 2156
BseMII CTCAG 1 cut(s) 1983
BseNI ACTGG 3 cut(s) 1312, 1336, 2149
BseRI GAGGAG 3 cut(s) 638, 911, 1232
BseSI GKGCMC 2 cut(s) 1385, 1735
BsgI GTGCAG 1 cut(s) 171
Bsh1236I CGCG 2 cut(s) 583, 903
BshFI GGCC 2 cut(s) 1598, 2223
BsiHKAI GWGCWC 1 cut(s) 1385
BsiHKCI CYCGRG 2 cut(s) 1684, 1937
BsiSI CCGG 4 cut(s) 71, 266, 787, 1665
BslFI GGGAC 2 cut(s) 581, 1953
BslI CCNNNNNNNGG 4 cut(s) 870, 1564, 1846, 2144
BsmAI GTCTC 2 cut(s) 1854, 1940
BsmFI GGGAC 2 cut(s) 581, 1953
BsnI GGCC 2 cut(s) 1598, 2223
Bso31I GGTCTC 1 cut(s) 1940
BsoBI CYCGRG 2 cut(s) 1684, 1937
Bsp119I TTCGAA 1 cut(s) 634
Bsp1286I GDGCHC 3 cut(s) 1385, 1735, 2019
Bsp143I GATC 8 cut(s) 481, 523, 1006, 1119, 1276, 1310, 2088, 2207
BspACI CCGC 6 cut(s) 237, 283, 323, 460, 903, 1759
BspANI GGCC 2 cut(s) 1598, 2223
BspCNI CTCAG 1 cut(s) 1984
BspFNI CGCG 2 cut(s) 583, 903
BspHI TCATGA 2 cut(s) 484, 1249
BspLI GGNNCC 2 cut(s) 287, 1851
BspMAI CTGCAG 1 cut(s) 1167
BspMI ACCTGC 2 cut(s) 1992, 2192
BspPI GGATC 2 cut(s) 1318, 2096
BspQI GCTCTTC 1 cut(s) 661
BspT104I TTCGAA 1 cut(s) 634
BspTI CTTAAG 2 cut(s) 604, 2230
BspTNI GGTCTC 1 cut(s) 1940
BsrDI GCAATG 4 cut(s) 50, 239, 267, 2156
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 3 cut(s) 1312, 1336, 2149
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 7 cut(s) 117, 1391, 1422, 1751, 2138, 2224, 2238
BssMI GATC 8 cut(s) 481, 523, 1006, 1119, 1276, 1310, 2088, 2207
BssT1I CCWWGG 2 cut(s) 2138, 2238
Bst2UI CCWGG 6 cut(s) 119, 1214, 1423, 1752, 1857, 2226
Bst4CI ACNGT 5 cut(s) 370, 644, 959, 1507, 2108
Bst6I CTCTTC 6 cut(s) 392, 504, 557, 615, 661, 914
BstAFI CTTAAG 2 cut(s) 604, 2230
BstAPI GCANNNNNTGC 2 cut(s) 185, 1823
BstBI TTCGAA 1 cut(s) 634
BstC8I GCNNGC 3 cut(s) 1570, 1906, 2001
BstDEI CTNAG 2 cut(s) 1807, 1992
BstDSI CCRYGG 1 cut(s) 1391
BstF5I GGATG 9 cut(s) 279, 496, 721, 733, 879, 1132, 1156, 1597, 2154
BstFNI CGCG 2 cut(s) 583, 903
BstH2I RGCGCY 1 cut(s) 946
BstHHI GCGC 2 cut(s) 903, 945
BstKTI GATC 8 cut(s) 484, 526, 1009, 1122, 1279, 1313, 2091, 2210
BstMAI GTCTC 2 cut(s) 1854, 1940
BstMBI GATC 8 cut(s) 481, 523, 1006, 1119, 1276, 1310, 2088, 2207
BstMWI GCNNNNNNNGC 6 cut(s) 185, 466, 1796, 1820, 1823, 1993
BstNI CCWGG 6 cut(s) 119, 1214, 1423, 1752, 1857, 2226
BstSCI CCNGG 8 cut(s) 117, 786, 1212, 1421, 1663, 1750, 1855, 2224
BstSFI CTRYAG 2 cut(s) 1163, 1573
BstSLI GKGCMC 2 cut(s) 1385, 1735
BstUI CGCG 2 cut(s) 583, 903
BstV2I GAAGAC 3 cut(s) 29, 1095, 1864
BstX2I RGATCY 1 cut(s) 1310
BstYI RGATCY 1 cut(s) 1310
BsuI GTATCC 2 cut(s) 53, 2254
BsuRI GGCC 2 cut(s) 1598, 2223
BtgI CCRYGG 1 cut(s) 1391
BtrI CACGTC 1 cut(s) 1494
BtsCI GGATG 9 cut(s) 279, 496, 721, 733, 879, 1132, 1156, 1597, 2154
BtsIMutI CAGTG 3 cut(s) 375, 649, 771
BveI ACCTGC 2 cut(s) 1992, 2192
Cac8I GCNNGC 3 cut(s) 1570, 1906, 2001
CaiI CAGNNNCTG 2 cut(s) 1799, 2225
CciI TCATGA 2 cut(s) 484, 1249
CfoI GCGC 2 cut(s) 903, 945
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 3 cut(s) 285, 1597, 2222
CseI GACGC 2 cut(s) 147, 578
Csp6I GTAC 2 cut(s) 868, 1921
CviQI GTAC 2 cut(s) 868, 1921
DdeI CTNAG 2 cut(s) 1807, 1992
DpnI GATC 8 cut(s) 483, 525, 1008, 1121, 1278, 1312, 2090, 2209
DpnII GATC 8 cut(s) 481, 523, 1006, 1119, 1276, 1310, 2088, 2207
DrdI GACNNNNNNGTC 3 cut(s) 31, 140, 1868
DriI GACNNNNNGTC 1 cut(s) 1932
DseDI GACNNNNNNGTC 3 cut(s) 31, 140, 1868
Eam1104I CTCTTC 6 cut(s) 392, 504, 557, 615, 661, 914
Eam1105I GACNNNNNGTC 1 cut(s) 1932
EarI CTCTTC 6 cut(s) 392, 504, 557, 615, 661, 914
EciI GGCGGA 1 cut(s) 475
Eco130I CCWWGG 2 cut(s) 2138, 2238
Eco24I GRGCYC 1 cut(s) 2019
Eco31I GGTCTC 1 cut(s) 1940
Eco32I GATATC 1 cut(s) 1950
Eco47I GGWCC 1 cut(s) 285
Eco47III AGCGCT 1 cut(s) 944
Eco57I CTGAAG 3 cut(s) 41, 795, 2046
Eco88I CYCGRG 2 cut(s) 1684, 1937
EcoO109I RGGNCCY 1 cut(s) 2222
EcoRI GAATTC 1 cut(s) 782
EcoRII CCWGG 6 cut(s) 117, 1212, 1421, 1750, 1855, 2224
EcoRV GATATC 1 cut(s) 1950
EcoT14I CCWWGG 2 cut(s) 2138, 2238
EcoT38I GRGCYC 1 cut(s) 2019
ErhI CCWWGG 2 cut(s) 2138, 2238
FalI AAGNNNNNCTT 4 cut(s) 587, 619, 655, 687
FaqI GGGAC 2 cut(s) 581, 1953
FauI CCCGC 1 cut(s) 230
FbaI TGATCA 2 cut(s) 481, 523
FokI GGATG 9 cut(s) 286, 503, 728, 740, 886, 1139, 1163, 1604, 2161
FriOI GRGCYC 1 cut(s) 2019
FspBI CTAG 2 cut(s) 842, 2034
GlaI GCGC 2 cut(s) 902, 944
GsuI CTGGAG 1 cut(s) 1878
HaeII RGCGCY 1 cut(s) 946
HaeIII GGCC 2 cut(s) 1598, 2223
HapII CCGG 4 cut(s) 71, 266, 787, 1665
HgaI GACGC 2 cut(s) 147, 578
HhaI GCGC 2 cut(s) 903, 945
Hin6I GCGC 2 cut(s) 901, 943
HinP1I GCGC 2 cut(s) 901, 943
HindIII AAGCTT 2 cut(s) 137, 1448
HinfI GANTC 6 cut(s) 302, 390, 500, 1184, 1330, 2153
HpaII CCGG 4 cut(s) 71, 266, 787, 1665
HphI GGTGA 6 cut(s) 224, 280, 329, 659, 853, 1129
Hpy166II GTNNAC 3 cut(s) 133, 1170, 1383
Hpy188III TCNNGA 9 cut(s) 394, 485, 675, 842, 1250, 1433, 1684, 1886, 1939
Hpy8I GTNNAC 3 cut(s) 133, 1170, 1383
HpyAV CCTTC 3 cut(s) 650, 752, 2021
HpyCH4III ACNGT 5 cut(s) 370, 644, 959, 1507, 2108
HpyCH4IV ACGT 2 cut(s) 448, 1493
HpyF10VI GCNNNNNNNGC 6 cut(s) 185, 466, 1796, 1820, 1823, 1993
HpyF3I CTNAG 2 cut(s) 1807, 1992
HpySE526I ACGT 2 cut(s) 448, 1493
HspAI GCGC 2 cut(s) 901, 943
Ksp22I TGATCA 2 cut(s) 481, 523
Kzo9I GATC 8 cut(s) 481, 523, 1006, 1119, 1276, 1310, 2088, 2207
LguI GCTCTTC 1 cut(s) 661
LmnI GCTCC 4 cut(s) 547, 817, 898, 1266
LweI GCATC 7 cut(s) 267, 310, 864, 871, 1690, 1801, 2192
MaeI CTAG 2 cut(s) 842, 2034
MaeII ACGT 2 cut(s) 448, 1493
MaeIII GTNAC 5 cut(s) 364, 797, 859, 907, 2164
MalI GATC 8 cut(s) 483, 525, 1008, 1121, 1278, 1312, 2090, 2209
MboI GATC 8 cut(s) 481, 523, 1006, 1119, 1276, 1310, 2088, 2207
MfeI CAATTG 1 cut(s) 1818
MflI RGATCY 1 cut(s) 1310
MhlI GDGCHC 3 cut(s) 1385, 1735, 2019
MlyI GAGTC 3 cut(s) 509, 1193, 2162
MmeI TCCRAC 1 cut(s) 279
MroXI GAANNNNTTC 2 cut(s) 670, 1208
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 283
MspCI CTTAAG 2 cut(s) 604, 2230
MspI CCGG 4 cut(s) 71, 266, 787, 1665
MspR9I CCNGG 8 cut(s) 119, 788, 1214, 1423, 1665, 1752, 1857, 2226
MunI CAATTG 1 cut(s) 1818
MvaI CCWGG 6 cut(s) 119, 1214, 1423, 1752, 1857, 2226
MvnI CGCG 2 cut(s) 583, 903
MwoI GCNNNNNNNGC 6 cut(s) 185, 466, 1796, 1820, 1823, 1993
NciI CCSGG 2 cut(s) 788, 1665
NdeII GATC 8 cut(s) 481, 523, 1006, 1119, 1276, 1310, 2088, 2207
NlaIV GGNNCC 2 cut(s) 287, 1851
NmuCI GTSAC 2 cut(s) 859, 2164
NspV TTCGAA 1 cut(s) 634
PaeR7I CTCGAG 1 cut(s) 1684
PagI TCATGA 2 cut(s) 484, 1249
PciSI GCTCTTC 1 cut(s) 661
PcsI WCGNNNNNNNCGW 1 cut(s) 2212
PdmI GAANNNNTTC 2 cut(s) 670, 1208
PfeI GAWTC 3 cut(s) 302, 390, 1330
PflMI CCANNNNNTGG 1 cut(s) 2144
PfoI TCCNGGA 2 cut(s) 786, 1212
PleI GAGTC 3 cut(s) 508, 1192, 2161
PpsI GAGTC 3 cut(s) 508, 1192, 2161
Psp6I CCWGG 6 cut(s) 117, 1212, 1421, 1750, 1855, 2224
PspGI CCWGG 6 cut(s) 117, 1212, 1421, 1750, 1855, 2224
PspN4I GGNNCC 2 cut(s) 287, 1851
PspPI GGNCC 3 cut(s) 285, 1597, 2222
PsrI GAACNNNNNNTAC 2 cut(s) 1600, 1632
PstI CTGCAG 1 cut(s) 1167
PstNI CAGNNNCTG 2 cut(s) 1799, 2225
PsuI RGATCY 1 cut(s) 1310
RsaI GTAC 2 cut(s) 869, 1922
RsaNI GTAC 2 cut(s) 868, 1921
RseI CAYNNNNRTG 1 cut(s) 489
SapI GCTCTTC 1 cut(s) 661
Sau3AI GATC 8 cut(s) 481, 523, 1006, 1119, 1276, 1310, 2088, 2207
Sau96I GGNCC 3 cut(s) 285, 1597, 2222
SchI GAGTC 3 cut(s) 509, 1193, 2162
ScrFI CCNGG 8 cut(s) 119, 788, 1214, 1423, 1665, 1752, 1857, 2226
SduI GDGCHC 3 cut(s) 1385, 1735, 2019
SfaNI GCATC 7 cut(s) 267, 310, 864, 871, 1690, 1801, 2192
SfcI CTRYAG 2 cut(s) 1163, 1573
Sfr274I CTCGAG 1 cut(s) 1684
SfuI TTCGAA 1 cut(s) 634
SinI GGWCC 1 cut(s) 285
SlaI CTCGAG 1 cut(s) 1684
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 5 cut(s) 604, 1181, 1431, 1684, 2230
SmoI CTYRAG 5 cut(s) 604, 1181, 1431, 1684, 2230
SsiI CCGC 6 cut(s) 237, 283, 323, 460, 903, 1759
SspI AATATT 1 cut(s) 813
SspMI CTAG 2 cut(s) 842, 2034
StyD4I CCNGG 8 cut(s) 117, 786, 1212, 1421, 1663, 1750, 1855, 2224
StyI CCWWGG 2 cut(s) 2138, 2238
TaaI ACNGT 5 cut(s) 370, 644, 959, 1507, 2108
TaiI ACGT 2 cut(s) 451, 1496
TatI WGTACW 2 cut(s) 867, 1920
TfiI GAWTC 3 cut(s) 302, 390, 1330
TscAI CASTG 3 cut(s) 375, 649, 778
TseFI GTSAC 2 cut(s) 859, 2164
Tsp45I GTSAC 2 cut(s) 859, 2164
TspDTI ATGAA 6 cut(s) 732, 756, 989, 1101, 1238, 2138
TspRI CASTG 3 cut(s) 375, 649, 778
Van91I CCANNNNNTGG 1 cut(s) 2144
Vha464I CTTAAG 2 cut(s) 604, 2230
VneI GTGCAC 1 cut(s) 1381
VpaK11BI GGWCC 1 cut(s) 285
XapI RAATTY 5 cut(s) 146, 782, 1034, 1544, 2007
XbaI TCTAGA 1 cut(s) 841
XhoI CTCGAG 1 cut(s) 1684
XmnI GAANNNNTTC 2 cut(s) 670, 1208
XspI CTAG 2 cut(s) 842, 2034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.