Rorug07G0244200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000246
Physical Location & Seq
Reverse (-)
22554095 .. 22555380
1286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0244200.1

Sequence Viewer

Length: 924 bp
ATGCTGGGAATGTCAACGAATTCCAATAGTTACCCTCCACCGGAAAATATCAGTTCCTCTCCATCGATCACTGTCCTTTTGACCGTCCTCCTCCTCGTCTTCTTCTTTCTTGGTTTCTTTTCTATTTACTTTTGCAAGTGTTTCATGGAAAACGTCCTCACGTCTTTTCAGCGCACACCCTCCGGCGACCTTGTGGGCGTACCTGTTCCTCCCAAAGAAGGCCTCGATCCCTCTCTAATTCAATCATTTCCTACCTTCATCTACGCAAGTGTCAAAGATTTTCGCATAGAGAAGTACGGTCTAGAATGTGCAATTTGCTTGGTGGAGTTTGAGGATGATAGCATGCTACGTCTTATAACAGCTTGTTGCCATGTCTTCCACCAAGAATGCATCGACTTGTGGCTCGAGTCTCACAAAACATGTCCCTTTTGCCGTGGAAACCTTGAATTGTCGCCAACTATAATGGACAAGTCTCCAGTGCTTGCTCATGAGAATGCCATGCACGAAATTAATGAAGGTGAAGAGTCCATATACGGTCAAGATGTTATCCGAATTGACATTAAAGACGAAGACGAAGAAGGCCGAGAAGGAAATCGTAGTGATCATCAACAAAATTTGGCTGCTAATGTAAATTCTAACGTGGAAGGTCAACAAATTGATGGACAAAATGAAGAGAACGAAACGGAAAGATTTTCGAGGTCACACTCGACAGGACACTCCATAGTTAGGCCTAGACAAGAGGAGGATAGGTATACACTCAGATTACCAGAGCATGTGAAGATAAAACTTCTTAGAGGCCACAATTGGACAGGAAGTTGTACTACATTTGGGGAGTTTTCACGACAAAGAGGCAGTTGTAAAGGTGGCTTCGGAGAGGTCTCAGAATCTAGCTCTGCTGGTGGAGAGTTTAGTAGAAAACTGTAA

Protein Analysis

307

Amino Acids

34.56

Weight (kDa)

5.02

Isoelectric Point (pI)

61.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 102 - 145 1.6e-13 Ring finger domain
zf-C3HC4_2 PF13923 102 - 144 1.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 102 - 131 6.3e-08 RING-like zinc finger
zf-C3HC4 PF00097 103 - 144 9e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 106 - 145 1.3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44080 AT1G57906 AT1G65735 AT1G65740 AT1G65760 AT1G65770 AT2G17690 AT2G26160 AT3G25750 AT3G25750 AT4G35733
fragaria_vesca FvH4_1g10810 FvH4_1g10830 FvH4_1g10840 FvH4_1g10840 FvH4_1g10840 FvH4_1g10860 FvH4_1g10880
malus_domestica MD02G1122000.v1.1 MD15G1235700.v1.1 MD15G1235900.v1.1
prunus_persica Prupe.7G177000_v2.0.a1 Prupe.7G177100_v2.0.a1 Prupe.7G177200_v2.0.a1 Prupe.7G177300_v2.0.a1 Prupe.7G177400_v2.0.a1 Prupe.7G177400_v2.0.a1
pyrus_communis pycom02g09500 pycom15g20990 pycom15g21000
rosa_chinensis RchiOBHm_Chr1g0325601 RchiOBHm_Chr2g0098071 RchiOBHm_Chr2g0098081 RchiOBHm_Chr2g0098091 RchiOBHm_Chr2g0098121 RchiOBHm_Chr2g0098131 RchiOBHm_Chr2g0098151 RchiOBHm_Chr2g0134711 RchiOBHm_Chr3g0456141 RchiOBHm_Chr4g0394811 RchiOBHm_Chr4g0416561 RchiOBHm_Chr5g0014321
rosa_laevigata RLG00000001178 RLG00000016753 RLG00000016754 RLG00000016755 RLG00000016756 RLG00000016757 RLG00000016758 RLG00000016760 RLG00000016761 RLG00000016762 RLG00000016763
rosa_multiflora Rmu_co8307469.1_g000001 Rmu_sc0000427.1_g000002 Rmu_sc0002880.1_g000004 Rmu_sc0002880.1_g000007 Rmu_sc0009178.1_g000019 Rmu_sc0013919.1_g000002 Rmu_sc0013919.1_g000003 Rmu_sc0014278.1_g000001 Rmu_sc0031755.1_g000001 Rmu_sc0031755.1_g000002 Rmu_sc0039043.1_g000001 Rmu_sc0039432.1_g000001
rosa_roxburghii Rroxscaffold_2G00144260 Rroxscaffold_2G00144270 Rroxscaffold_2G00144290 Rroxscaffold_2G00144310 Rroxscaffold_2G00144320 Rroxscaffold_2G00144340 Rroxscaffold_3G00238070
rosa_rugosa Rorug02G0069800 Rorug02G0069900 Rorug02G0070000 Rorug02G0070100 Rorug02G0070200 Rorug02G0070500 Rorug02G0070500 Rorug05G0053500 Rorug05G0482400 Rorug07G0244200
rosa_samantha Rh1DG210300 Rh2AG117100 Rh2AG117200 Rh2AG117300 Rh2AG117500 Rh2AG117700 Rh2AG117800 Rh2AG563300 Rh2BG119900 Rh2BG120000 Rh2BG120200 Rh2BG120300 Rh2BG120400 Rh2BG120600 Rh2BG120700 Rh2BG377200 Rh2CG076100 Rh2CG076200 Rh2CG121600 Rh2CG121700 Rh2CG121900 Rh2CG122000 Rh2CG122100 Rh2CG122200 Rh2CG122300 Rh2DG121600 Rh2DG121700 Rh2DG122000 Rh2DG122100 Rh2DG122300 Rh3AG138900 Rh3BG074100 Rh3CG073500 Rh3DG298600 Rh4CG222800 Rh4CG222900 Rh5CG120400 Rh6BG018400 Rh6BG124600 Rh6CG123200 Rh6CG123300 Rh7AG342600 Rh7AG342700 Rh7BG352100 Rh7CG128700 Rh7CG360200 Rh7CG360300
rosa_wichuraiana Rw2G009150 Rw2G009160 Rw2G009170 Rw2G009200 Rw4G016710 Rw5G035380 Rw6G043260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 356
AccI GTMKAC 1 cut(s) 752
AclWI GGATC 1 cut(s) 221
AcsI RAATTY 3 cut(s) 19, 613, 631
AfaI GTAC 3 cut(s) 201, 296, 820
AfiI CCNNNNNNNGG 3 cut(s) 40, 218, 726
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 2 cut(s) 242, 446
AjiI CACGTC 1 cut(s) 162
AluBI AGCT 2 cut(s) 362, 891
AluI AGCT 2 cut(s) 362, 891
Alw26I GTCTC 3 cut(s) 414, 477, 883
AlwI GGATC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 4 cut(s) 220, 580, 728, 796
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 3 cut(s) 19, 613, 631
AseI ATTAAT 1 cut(s) 510
AspLEI GCGC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 404
BarI GAAGNNNNNNTAC 2 cut(s) 805, 837
BbsI GAAGAC 3 cut(s) 91, 367, 576
BbvI GCAGC 1 cut(s) 607
BccI CCATC 2 cut(s) 70, 653
BceAI ACGGC 1 cut(s) 417
BclI TGATCA 1 cut(s) 601
BcoDI GTCTC 3 cut(s) 414, 477, 883
BfaI CTAG 3 cut(s) 302, 732, 888
BisI GCNGC 1 cut(s) 621
BlsI GCNGC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 404
BmgBI CACGTC 1 cut(s) 162
BmsI GCATC 1 cut(s) 399
BpiI GAAGAC 3 cut(s) 91, 367, 576
BpmI CTGGAG 1 cut(s) 459
Bsa29I ATCGAT 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 883
BsaJI CCNNGG 1 cut(s) 433
BsaWI WCCGGW 1 cut(s) 40
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 218, 726
Bse1I ACTGG 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 3 cut(s) 40, 218, 726
BseMII CTCAG 2 cut(s) 772, 894
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 3 cut(s) 80, 83, 755
BseXI GCAGC 1 cut(s) 607
BseYI CCCAGC 1 cut(s) 4
BshFI GGCC 4 cut(s) 222, 582, 730, 798
BshVI ATCGAT 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 2 cut(s) 41, 183
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 3 cut(s) 40, 218, 726
BsmAI GTCTC 3 cut(s) 414, 477, 883
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 2 cut(s) 392, 499
BsnI GGCC 4 cut(s) 222, 582, 730, 798
Bso31I GGTCTC 1 cut(s) 883
BsoBI CYCGRG 1 cut(s) 404
Bsp143I GATC 3 cut(s) 66, 226, 601
BspANI GGCC 4 cut(s) 222, 582, 730, 798
BspCNI CTCAG 2 cut(s) 771, 893
BspDI ATCGAT 1 cut(s) 65
BspHI TCATGA 1 cut(s) 487
BspPI GGATC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 883
BsrI ACTGG 1 cut(s) 476
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 3 cut(s) 66, 226, 601
BssNAI GTATAC 1 cut(s) 753
Bst1107I GTATAC 1 cut(s) 753
Bst4CI ACNGT 5 cut(s) 73, 85, 299, 536, 921
Bst6I CTCTTC 2 cut(s) 516, 666
BstC8I GCNNGC 2 cut(s) 344, 483
BstDEI CTNAG 3 cut(s) 758, 791, 880
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 1 cut(s) 340
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 3 cut(s) 69, 229, 604
BstMAI GTCTC 3 cut(s) 414, 477, 883
BstMBI GATC 3 cut(s) 66, 226, 601
BstNSI RCATGY 3 cut(s) 346, 423, 776
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 3 cut(s) 91, 367, 576
BstZ17I GTATAC 1 cut(s) 753
Bsu15I ATCGAT 1 cut(s) 65
BsuRI GGCC 4 cut(s) 222, 582, 730, 798
BsuTUI ATCGAT 1 cut(s) 65
BtgI CCRYGG 1 cut(s) 433
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 69, 483
Cac8I GCNNGC 2 cut(s) 344, 483
CciI TCATGA 1 cut(s) 487
CfoI GCGC 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 65
Csp6I GTAC 3 cut(s) 200, 295, 819
CviAII CATG 7 cut(s) 145, 343, 371, 420, 488, 499, 773
CviJI RGCY 9 cut(s) 222, 362, 403, 582, 620, 730, 798, 867, 891
CviKI_1 RGCY 9 cut(s) 222, 362, 403, 582, 620, 730, 798, 867, 891
CviQI GTAC 3 cut(s) 200, 295, 819
DdeI CTNAG 3 cut(s) 758, 791, 880
DpnI GATC 3 cut(s) 68, 228, 603
DpnII GATC 3 cut(s) 66, 226, 601
Eam1104I CTCTTC 2 cut(s) 516, 666
EarI CTCTTC 2 cut(s) 516, 666
Eco147I AGGCCT 2 cut(s) 222, 730
Eco31I GGTCTC 1 cut(s) 883
Eco88I CYCGRG 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 19
EcoT22I ATGCAT 1 cut(s) 392
FaeI CATG 7 cut(s) 148, 346, 374, 423, 491, 502, 776
FaqI GGGAC 1 cut(s) 408
FatI CATG 7 cut(s) 144, 342, 370, 419, 487, 498, 772
FbaI TGATCA 1 cut(s) 601
FblI GTMKAC 1 cut(s) 752
Fnu4HI GCNGC 1 cut(s) 621
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 621
FspBI CTAG 3 cut(s) 302, 732, 888
GlaI GCGC 1 cut(s) 173
GluI GCNGC 1 cut(s) 621
GsaI CCCAGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 4 cut(s) 222, 582, 730, 798
HapII CCGG 2 cut(s) 41, 183
HhaI GCGC 1 cut(s) 174
Hin1II CATG 7 cut(s) 148, 346, 374, 423, 491, 502, 776
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 2 cut(s) 15, 650
HindII GTYRAC 2 cut(s) 15, 650
HinfI GANTC 3 cut(s) 407, 524, 884
HpaII CCGG 2 cut(s) 41, 183
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 3 cut(s) 15, 650, 753
Hpy188I TCNGA 4 cut(s) 551, 761, 872, 883
Hpy188III TCNNGA 4 cut(s) 302, 488, 539, 840
Hpy8I GTNNAC 3 cut(s) 15, 650, 753
HpyAV CCTTC 6 cut(s) 212, 265, 509, 572, 581, 638
HpyCH4III ACNGT 5 cut(s) 73, 85, 299, 536, 921
HpyCH4IV ACGT 4 cut(s) 153, 161, 349, 639
HpyCH4V TGCA 4 cut(s) 135, 311, 390, 502
HpyF3I CTNAG 3 cut(s) 758, 791, 880
HpySE526I ACGT 4 cut(s) 153, 161, 349, 639
Hsp92II CATG 7 cut(s) 148, 346, 374, 423, 491, 502, 776
HspAI GCGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 3 cut(s) 66, 226, 601
LpnPI CCDG 8 cut(s) 54, 196, 216, 489, 696, 780, 795, 882
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 1 cut(s) 399
MaeI CTAG 3 cut(s) 302, 732, 888
MaeII ACGT 4 cut(s) 153, 161, 349, 639
MaeIII GTNAC 2 cut(s) 29, 699
MalI GATC 3 cut(s) 68, 228, 603
MboI GATC 3 cut(s) 66, 226, 601
MboII GAAGA 8 cut(s) 91, 94, 367, 533, 581, 587, 683, 790
MfeI CAATTG 1 cut(s) 802
MlyI GAGTC 2 cut(s) 416, 533
Mph1103I ATGCAT 1 cut(s) 392
MseI TTAA 2 cut(s) 510, 561
MslI CAYNNNNRTG 1 cut(s) 492
MspI CCGG 2 cut(s) 41, 183
MunI CAATTG 1 cut(s) 802
Mva1269I GAATGC 2 cut(s) 392, 499
NdeII GATC 3 cut(s) 66, 226, 601
NlaIII CATG 7 cut(s) 148, 346, 374, 423, 491, 502, 776
NmeAIII GCCGAG 1 cut(s) 608
NmuCI GTSAC 1 cut(s) 699
NsiI ATGCAT 1 cut(s) 392
NspI RCATGY 3 cut(s) 346, 423, 776
PaeI GCATGC 1 cut(s) 346
PaeR7I CTCGAG 1 cut(s) 404
PagI TCATGA 1 cut(s) 487
PceI AGGCCT 2 cut(s) 222, 730
PciI ACATGT 1 cut(s) 419
PctI GAATGC 2 cut(s) 392, 499
PfeI GAWTC 1 cut(s) 884
PkrI GCNGC 1 cut(s) 622
PleI GAGTC 2 cut(s) 415, 532
PpsI GAGTC 2 cut(s) 415, 532
PscI ACATGT 1 cut(s) 419
PshBI ATTAAT 1 cut(s) 510
PsiI TTATAA 1 cut(s) 356
PspFI CCCAGC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 404
RsaI GTAC 3 cut(s) 201, 296, 820
RsaNI GTAC 3 cut(s) 200, 295, 819
RseI CAYNNNNRTG 1 cut(s) 492
SaqAI TTAA 2 cut(s) 510, 561
SatI GCNGC 1 cut(s) 621
Sau3AI GATC 3 cut(s) 66, 226, 601
SchI GAGTC 2 cut(s) 416, 533
SfaNI GCATC 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 404
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SphI GCATGC 1 cut(s) 346
SseBI AGGCCT 2 cut(s) 222, 730
SspMI CTAG 3 cut(s) 302, 732, 888
StuI AGGCCT 2 cut(s) 222, 730
TaaI ACNGT 5 cut(s) 73, 85, 299, 536, 921
TaiI ACGT 4 cut(s) 156, 164, 352, 642
TaqI TCGA 6 cut(s) 65, 225, 393, 405, 695, 707
TatI WGTACW 1 cut(s) 818
TfiI GAWTC 1 cut(s) 884
Tru1I TTAA 2 cut(s) 510, 561
Tru9I TTAA 2 cut(s) 510, 561
TscAI CASTG 2 cut(s) 76, 483
TseFI GTSAC 1 cut(s) 699
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 1 cut(s) 699
TspDTI ATGAA 4 cut(s) 133, 247, 528, 684
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 2 cut(s) 76, 483
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 3 cut(s) 19, 613, 631
XbaI TCTAGA 1 cut(s) 301
XceI RCATGY 3 cut(s) 346, 423, 776
XhoI CTCGAG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 752
XspI CTAG 3 cut(s) 302, 732, 888
Zsp2I ATGCAT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.