Rorug02G0070200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000246
Physical Location & Seq
Reverse (-)
5484254 .. 5491188
6935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0070200.1

Sequence Viewer

Length: 3021 bp
ATGGATTTACTTCCGGGAGGGAGTTCAAGCATGGTAAAGAAAACCGGGTTCAGGTCACTGAAGCTGGTGAACATAGAGATGGACCAAGTTCTGGGAGACAAGCCCGTCGGAGTTGACTATGGCAGGCTTGATAATGGTCTCTCTTACTATGTTCGTTGCAATTCTAAGCCCAAAATGAGAGCAGCTCTTGCCCTCGCTGTTAAAGTCGGGTCAGTTTTGGAAGAGGAGGAAGAACGAGGAGTTGCCCACATTGTTGAGCATCTTGCTTTCAGTGCTACTCAGAAGTACACAAATCATGATATAGTCAGATTTCTTGAAAGTATTGGGGCAGAATTTGGCGCTTGCCAGAATGCAGTGACATCGGCTGATGACACTGTTTATGAGTTATTTGTTCCTGTTGACAAGCTTGAACTATTATCTGAGGCCATTTCTGTCTTGGCGGAATTCAGTTCTGAGATTCGTGTCTCAAAAGACGATGTGGAAAGAGAAAGAGGCGCTGTTATGGAAGAGTACAGAGGGAATCGAAACGCTACGGGAAGGATGCAGGATGCGCATTGGCTTTTAATGATGGCTGGTTCAAAGTATGCTGAGCGTTTACCTATTGGATTAGAAAAGGTAATTCGAACTGTTTCTCCTGAGACTGTGAAGCAATTTTATCAGAAGTGGTACCATTTAAGCAATATGGCAGTGATAGCTGTAGGAGATTTCCCAGATACTGAGAGTGTTGTTGAGTTGATAAAGAATCAATTTGGGGATAAAAGTTCTGCACCTGAGCGTCCACTTATACCAACATATCAAGTTCCATCTCATGAAGAGCCACGTTATTCTTGTTTCATTGAATCTGAGGCAACTGGGTCTGCAGTGATTATTAGCTATAAGACTCCAGCTGTTGAGCTGAATACAGTGAGGGACTATAGGGATTTACTGGCAGAATCAATGTTCCTTTATGCTCTAAACCAGAGGTTCTTTAAAATAGCTCGTAGAAAAGATCCACCCTTTTTCTCATGCTCAACTTCCGCAGATGTTCTAGTTAATCCCTTGAAGGCTTATATGATGACCTCATCTTGTAAAGAAAAGGGAACAATTCAGGCCCTAGAGTCGATGCTGATTGAGGTTGCCAGGGTACGGCTACATGGGTTTTCAGAACGGGAAGTGTCTATAGTTCGAGCTTTACTGATGTCAGAGATTGAATCTGCATATTTGGAACGCGATCAAATGCAGTCAACTAGCTTGCGAGATGAATATTTACAGCATTTTCTCCGTAATGAACCTGTTATTGGGATTGAGTATGAGGCCCAACTCCAGAAGACCCTTCTACCTTATATAACAGCAGCAGAGATATCCAAATATGCTGAGAAGTTACAAACATCATGCAGCTGTGTCATTAAGACAATTGAACCACGAGCTTCTGCAATAGTAGAAGATTTGAAAAATGTTGTCTCTAAAATCAGTGCTCTTGAGGAAGAAAGAATCATTTCTCCATGGGATGAAGAACATATTCCAGAAGAAATTGTTAGCACAAAACCTAATCGAGGGAATATTGTGCAGCAATGTGAATATTCAAATATTGGAGCTACTGAATTGATTCTATCGAATGGCATGAGAGTTTGCTATAAGAGTACAGACTTTCTTGATGATCAGGTTATCTTTACAGGGTTCTCATATGGGGGTTTATCCGAACTTGTTGAAAGTGAGTACTTTTCTTGCTCGATGGGGCCAACTATTGCAGGAGAAATTGGGGTATATGGTTATAGACCTTCAGTTCTGATGGATATGCTTGCGGGTAAGAGGTCCGAAGTCAGCACAAAGCTTGGAGCATACACGAGAACCTTTGCTGGTGATTGTTCACCCTCAGACTTAGAAACTGCCTTGCAGCTTGTGTATCAACTATTTACCACAAATGTAACACCAGGAGAAGAAGATGTCAAAATAGTGATGCAAATGGCAGAAGAAATGGTCCGTAATCAAGACAGGGATCCTTATACTGCATTTGCAAATCGTGTGAAGGAGCTGAACTATGGAAACTCCTATTTCTTTAGGCCTACCAGAATAAGTGATCTTCAGAAAGTTGATCCAATGAAAGCTTGTGAATATTTCAACAAATGTTTCAAGGATCCATCAACCTTCTCTATGGTCATTGTTGGGAATATTGATCCTTCTATAGCACTTCCTTTAATATTACAGTATTTGGGCGGAATACCAAAGCCTTCTGAACCTCTCATGCAATATAACCGCGATGACCTCACAGGACTGCCATTCACTTTTCCTAGAACCATAATAAGAGAAGTGGTGCGCAGCCCTATGGTGGAAGAACAATGTTCTGTCCAGCTTTGCTTTCCTGTGGAGCTGAATAATGGAACCATGGTAGAAGATATTCACGTTGTTGGTTTCCTGAGCAAACTTCTTGAAACGAAAATAATGCAAGTTCTCCGCTTCAAGCATGGACAGATCTACACTGTGGGTGTTTCAGTATTTCTTGGCGGTAATAAGCCTTCCAGAACTGCTAATGTTCGTGGAGATATAAGCATAAATTTTTCTTGTGATCCAGAAATTTCCTCGAAGCTGGTCGACCTTACTTTGGATGAAATATTACGTCTTCAAGAGGAAGGGCCTTCGGCTGAGGATGTCTCAACCATCCTTGAAATTGAGCAAAGAGCCCATGAAAATGGGATTCAGGAAAATTACTACTGGCTGGAGAGGATTTTACACAGCTACCAGTCAAGGATTTACTCTGGCGATGTTGGCACTTGTTTTGAGACTCAAGAGGAAGGTCGGTTAAAAGTTAGACAATCTCTGACCCCAAGAACGGCGCAGTTATCATTACAAAGCATACTACCTTATCCTTGCAAAAAACAGTACACTGTAGTAATCTTAATGCCCCGTACTTCTCGGTTTAAGTTACTAGATTCAATCTTTCGATCTACGACCAGTTTTGGTAGAGATGCTAAGATTTTAGCCGCGGTTGCTGGTCTCGCAGTTTTGGGTCTTAGCTTGTGGAGACGTTCACGAAGTGCGCGCTAA

Protein Analysis

1006

Amino Acids

113.23

Weight (kDa)

5.33

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 54 - 172 4.7e-24 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 210 - 390 6.4e-17 Peptidase M16 inactive domain
Peptidase_M16_C PF05193 696 - 879 2.4e-19 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44080 AT1G57906 AT1G65735 AT1G65740 AT1G65760 AT1G65770 AT2G17690 AT2G26160 AT3G25750 AT3G25750 AT4G35733
fragaria_vesca FvH4_1g10810 FvH4_1g10830 FvH4_1g10840 FvH4_1g10840 FvH4_1g10840 FvH4_1g10860 FvH4_1g10880
malus_domestica MD02G1122000.v1.1 MD15G1235700.v1.1 MD15G1235900.v1.1
prunus_persica Prupe.7G177000_v2.0.a1 Prupe.7G177100_v2.0.a1 Prupe.7G177200_v2.0.a1 Prupe.7G177300_v2.0.a1 Prupe.7G177400_v2.0.a1 Prupe.7G177400_v2.0.a1
pyrus_communis pycom02g09500 pycom15g20990 pycom15g21000
rosa_chinensis RchiOBHm_Chr1g0325601 RchiOBHm_Chr2g0098071 RchiOBHm_Chr2g0098081 RchiOBHm_Chr2g0098091 RchiOBHm_Chr2g0098121 RchiOBHm_Chr2g0098131 RchiOBHm_Chr2g0098151 RchiOBHm_Chr2g0134711 RchiOBHm_Chr3g0456141 RchiOBHm_Chr4g0394811 RchiOBHm_Chr4g0416561 RchiOBHm_Chr5g0014321
rosa_laevigata RLG00000001178 RLG00000016753 RLG00000016754 RLG00000016755 RLG00000016756 RLG00000016757 RLG00000016758 RLG00000016760 RLG00000016761 RLG00000016762 RLG00000016763
rosa_multiflora Rmu_co8307469.1_g000001 Rmu_sc0000427.1_g000002 Rmu_sc0002880.1_g000004 Rmu_sc0002880.1_g000007 Rmu_sc0009178.1_g000019 Rmu_sc0013919.1_g000002 Rmu_sc0013919.1_g000003 Rmu_sc0014278.1_g000001 Rmu_sc0031755.1_g000001 Rmu_sc0031755.1_g000002 Rmu_sc0039043.1_g000001 Rmu_sc0039432.1_g000001
rosa_roxburghii Rroxscaffold_2G00144260 Rroxscaffold_2G00144270 Rroxscaffold_2G00144290 Rroxscaffold_2G00144310 Rroxscaffold_2G00144320 Rroxscaffold_2G00144340 Rroxscaffold_3G00238070
rosa_rugosa Rorug02G0069800 Rorug02G0069900 Rorug02G0070000 Rorug02G0070100 Rorug02G0070200 Rorug02G0070500 Rorug02G0070500 Rorug05G0053500 Rorug05G0482400 Rorug07G0244200
rosa_samantha Rh1DG210300 Rh2AG117100 Rh2AG117200 Rh2AG117300 Rh2AG117500 Rh2AG117700 Rh2AG117800 Rh2AG563300 Rh2BG119900 Rh2BG120000 Rh2BG120200 Rh2BG120300 Rh2BG120400 Rh2BG120600 Rh2BG120700 Rh2BG377200 Rh2CG076100 Rh2CG076200 Rh2CG121600 Rh2CG121700 Rh2CG121900 Rh2CG122000 Rh2CG122100 Rh2CG122200 Rh2CG122300 Rh2DG121600 Rh2DG121700 Rh2DG122000 Rh2DG122100 Rh2DG122300 Rh3AG138900 Rh3BG074100 Rh3CG073500 Rh3DG298600 Rh4CG222800 Rh4CG222900 Rh5CG120400 Rh6BG018400 Rh6BG124600 Rh6CG123200 Rh6CG123300 Rh7AG342600 Rh7AG342700 Rh7BG352100 Rh7CG128700 Rh7CG360200 Rh7CG360300
rosa_wichuraiana Rw2G009150 Rw2G009160 Rw2G009170 Rw2G009200 Rw4G016710 Rw5G035380 Rw6G043260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
Acc16I TGCGCA 2 cut(s) 552, 2294
Acc65I GGTACC 1 cut(s) 666
AccB1I GGYRCC 1 cut(s) 666
AccB7I CCANNNNNTGG 1 cut(s) 91
AccI GTMKAC 1 cut(s) 2568
AccII CGCG 4 cut(s) 1209, 2235, 2960, 3016
AciI CCGC 9 cut(s) 440, 1017, 1781, 2193, 2233, 2431, 2481, 2958, 2960
AclWI GGATC 8 cut(s) 983, 1970, 1983, 2066, 2108, 2121, 2147, 2537
AcsI RAATTY 4 cut(s) 332, 443, 2530, 2550
AcuI CTGAAG 3 cut(s) 80, 1743, 2045
AdeI CACNNNGTG 1 cut(s) 3011
AfaI GTAC 8 cut(s) 287, 512, 668, 1125, 1621, 1697, 2858, 2884
AfiI CCNNNNNNNGG 8 cut(s) 51, 91, 1125, 1277, 1532, 2305, 2578, 2806
AjnI CCWGG 2 cut(s) 1118, 1909
AjuI GAANNNNNNNTTGG 2 cut(s) 1260, 1292
AloI GAACNNNNNNTCC 2 cut(s) 616, 648
Alw21I GWGCWC 1 cut(s) 1456
Alw26I GTCTC 9 cut(s) 90, 143, 469, 632, 1444, 2632, 2750, 2975, 2992
AlwI GGATC 8 cut(s) 983, 1970, 1983, 2066, 2108, 2121, 2147, 2537
AlwNI CAGNNNCTG 1 cut(s) 716
AoxI GGCC 6 cut(s) 423, 1089, 1293, 1715, 2039, 2609
ApeKI GCWGC 6 cut(s) 182, 1331, 1374, 1546, 1873, 2295
ApoI RAATTY 4 cut(s) 332, 443, 2530, 2550
Asp700I GAANNNNTTC 5 cut(s) 628, 1474, 1497, 1584, 2373
Asp718I GGTACC 1 cut(s) 666
AspLEI GCGC 7 cut(s) 341, 497, 553, 2295, 2812, 3016, 3018
AspS9I GGNCC 7 cut(s) 82, 1090, 1294, 1715, 1791, 1957, 2609
AsuC2I CCSGG 2 cut(s) 15, 46
AsuHPI GGTGA 3 cut(s) 79, 1839, 1850
AsuII TTCGAA 1 cut(s) 622
AvaII GGWCC 3 cut(s) 82, 1791, 1957
BamHI GGATCC 2 cut(s) 1975, 2113
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 1 cut(s) 2659
BarI GAAGNNNNNNTAC 2 cut(s) 2476, 2508
BauI CACGAG 2 cut(s) 1401, 1822
BbsI GAAGAC 2 cut(s) 1313, 2588
Bbv12I GWGCWC 1 cut(s) 1456
BbvCI CCTCAGC 1 cut(s) 2619
BbvI GCAGC 6 cut(s) 194, 1343, 1386, 1558, 1885, 2307
BccI CCATC 7 cut(s) 73, 562, 811, 1705, 1762, 2125, 2642
BceAI ACGGC 2 cut(s) 1142, 2823
BcgI CGANNNNNNTGC 4 cut(s) 342, 376, 2401, 2435
BciT130I CCWGG 2 cut(s) 1120, 1911
BclI TGATCA 1 cut(s) 1636
BcnI CCSGG 2 cut(s) 15, 46
BcoDI GTCTC 9 cut(s) 90, 143, 469, 632, 1444, 2632, 2750, 2975, 2992
BfaI CTAG 5 cut(s) 1028, 1094, 1227, 2268, 2903
BfmI CTRYAG 6 cut(s) 696, 858, 913, 1158, 2160, 2862
BfoI RGCGCY 2 cut(s) 342, 498
BglII AGATCT 1 cut(s) 2448
BisI GCNGC 7 cut(s) 183, 1332, 1375, 1547, 1874, 2296, 2958
BlpI GCTNAGC 1 cut(s) 588
BlsI GCNGC 7 cut(s) 184, 1333, 1376, 1548, 1875, 2297, 2959
BmcAI AGTACT 1 cut(s) 1697
Bme1390I CCNGG 4 cut(s) 15, 46, 1120, 1911
Bme18I GGWCC 3 cut(s) 82, 1791, 1957
BmgT120I GGNCC 7 cut(s) 82, 1090, 1294, 1715, 1791, 1957, 2609
BmiI GGNNCC 5 cut(s) 668, 1716, 1977, 2115, 2359
BmrFI CCNGG 4 cut(s) 15, 46, 1120, 1911
BmrI ACTGGG 1 cut(s) 861
BmsI GCATC 6 cut(s) 268, 531, 538, 1092, 1926, 2932
BmuI ACTGGG 1 cut(s) 861
BpiI GAAGAC 2 cut(s) 1313, 2588
BplI GAGNNNNNCTC 4 cut(s) 169, 201, 2612, 2644
BpmI CTGGAG 3 cut(s) 867, 1286, 2714
Bpu10I CCTNAGC 3 cut(s) 771, 2393, 2619
Bpu1102I GCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 622
BpuEI CTTGAG 2 cut(s) 1478, 2745
BpuMI CCSGG 2 cut(s) 15, 46
BsaI GGTCTC 2 cut(s) 143, 2975
BsaJI CCNNGG 4 cut(s) 1119, 1481, 2361, 2958
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 8 cut(s) 51, 91, 1125, 1277, 1532, 2305, 2578, 2806
Bse1I ACTGG 5 cut(s) 856, 930, 2693, 2716, 2928
Bse3DI GCAATG 1 cut(s) 1556
BseBI CCWGG 2 cut(s) 1120, 1911
BseDI CCNNGG 4 cut(s) 1119, 1481, 2361, 2958
BseGI GGATG 6 cut(s) 546, 553, 1492, 2587, 2629, 2634
BseLI CCNNNNNNNGG 8 cut(s) 51, 91, 1125, 1277, 1532, 2305, 2578, 2806
BseMI GCAATG 1 cut(s) 1556
BseNI ACTGG 5 cut(s) 856, 930, 2693, 2716, 2928
BsePI GCGCGC 1 cut(s) 3014
BseRI GAGGAG 2 cut(s) 239, 252
BseXI GCAGC 6 cut(s) 194, 1343, 1386, 1558, 1885, 2307
BsgI GTGCAG 2 cut(s) 750, 1565
Bsh1236I CGCG 4 cut(s) 1209, 2235, 2960, 3016
BshFI GGCC 6 cut(s) 425, 1091, 1295, 1717, 2041, 2611
BshNI GGYRCC 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 1456
BsiSI CCGG 2 cut(s) 14, 45
BslFI GGGAC 1 cut(s) 923
BslI CCNNNNNNNGG 8 cut(s) 51, 91, 1125, 1277, 1532, 2305, 2578, 2806
BsmAI GTCTC 9 cut(s) 90, 143, 469, 632, 1444, 2632, 2750, 2975, 2992
BsmBI CGTCTC 1 cut(s) 2992
BsmFI GGGAC 1 cut(s) 923
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 6 cut(s) 425, 1091, 1295, 1717, 2041, 2611
Bso31I GGTCTC 2 cut(s) 143, 2975
Bsp119I TTCGAA 1 cut(s) 622
Bsp1286I GDGCHC 2 cut(s) 1456, 2659
Bsp1720I GCTNAGC 1 cut(s) 588
Bsp19I CCATGG 2 cut(s) 1481, 2361
BspACI CCGC 9 cut(s) 440, 1017, 1781, 2193, 2233, 2431, 2481, 2958, 2960
BspANI GGCC 6 cut(s) 425, 1091, 1295, 1717, 2041, 2611
BspFNI CGCG 4 cut(s) 1209, 2235, 2960, 3016
BspHI TCATGA 2 cut(s) 295, 808
BspLI GGNNCC 5 cut(s) 668, 1716, 1977, 2115, 2359
BspMAI CTGCAG 1 cut(s) 862
BspPI GGATC 8 cut(s) 983, 1970, 1983, 2066, 2108, 2121, 2147, 2537
BspQI GCTCTTC 1 cut(s) 807
BspT104I TTCGAA 1 cut(s) 622
BspT107I GGYRCC 1 cut(s) 666
BspTNI GGTCTC 2 cut(s) 143, 2975
BsrDI GCAATG 1 cut(s) 1556
BsrI ACTGG 5 cut(s) 856, 930, 2693, 2716, 2928
BssECI CCNNGG 4 cut(s) 1119, 1481, 2361, 2958
BssHII GCGCGC 1 cut(s) 3014
BssSI CACGAG 2 cut(s) 1401, 1822
BssT1I CCWWGG 2 cut(s) 1481, 2361
Bst2BI CACGAG 2 cut(s) 1401, 1822
Bst2UI CCWGG 2 cut(s) 1120, 1911
Bst4CI ACNGT 8 cut(s) 376, 628, 643, 904, 2184, 2458, 2856, 2863
Bst6I CTCTTC 3 cut(s) 216, 501, 807
BstAPI GCANNNNNTGC 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 5 cut(s) 125, 343, 1232, 1779, 3016
BstDSI CCRYGG 3 cut(s) 1481, 2361, 2958
BstENI CCTNNNNNAGG 1 cut(s) 1530
BstF5I GGATG 6 cut(s) 546, 553, 1492, 2587, 2629, 2634
BstFNI CGCG 4 cut(s) 1209, 2235, 2960, 3016
BstH2I RGCGCY 2 cut(s) 342, 498
BstHHI GCGC 7 cut(s) 341, 497, 553, 2295, 2812, 3016, 3018
BstMAI GTCTC 9 cut(s) 90, 143, 469, 632, 1444, 2632, 2750, 2975, 2992
BstMWI GCNNNNNNNGC 7 cut(s) 188, 272, 550, 692, 2742, 2963, 2972
BstNI CCWGG 2 cut(s) 1120, 1911
BstSCI CCNGG 4 cut(s) 13, 44, 1118, 1909
BstSFI CTRYAG 6 cut(s) 696, 858, 913, 1158, 2160, 2862
BstUI CGCG 4 cut(s) 1209, 2235, 2960, 3016
BstV1I GCAGC 6 cut(s) 194, 1343, 1386, 1558, 1885, 2307
BstV2I GAAGAC 2 cut(s) 1313, 2588
BstX2I RGATCY 4 cut(s) 988, 1975, 2113, 2448
BstXI CCANNNNNNTGG 1 cut(s) 2666
BstYI RGATCY 4 cut(s) 988, 1975, 2113, 2448
BsuRI GGCC 6 cut(s) 425, 1091, 1295, 1717, 2041, 2611
BtgI CCRYGG 3 cut(s) 1481, 2361, 2958
BtgZI GCGATG 2 cut(s) 2250, 2751
BtsCI GGATG 6 cut(s) 546, 553, 1492, 2587, 2629, 2634
BtsI GCAGTG 3 cut(s) 360, 693, 867
Cac8I GCNNGC 5 cut(s) 125, 343, 1232, 1779, 3016
CaiI CAGNNNCTG 1 cut(s) 716
CciI TCATGA 2 cut(s) 295, 808
CfoI GCGC 7 cut(s) 341, 497, 553, 2295, 2812, 3016, 3018
Cfr13I GGNCC 7 cut(s) 82, 1090, 1294, 1715, 1791, 1957, 2609
Cfr42I CCGCGG 1 cut(s) 2961
CseI GACGC 1 cut(s) 764
Csp6I GTAC 8 cut(s) 286, 511, 667, 1124, 1620, 1696, 2857, 2883
CviQI GTAC 8 cut(s) 286, 511, 667, 1124, 1620, 1696, 2857, 2883
DraI TTTAAA 1 cut(s) 970
DraIII CACNNNGTG 1 cut(s) 3011
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
Eam1104I CTCTTC 3 cut(s) 216, 501, 807
EarI CTCTTC 3 cut(s) 216, 501, 807
EciI GGCGGA 2 cut(s) 455, 2208
Eco130I CCWWGG 2 cut(s) 1481, 2361
Eco147I AGGCCT 1 cut(s) 2041
Eco24I GRGCYC 1 cut(s) 2659
Eco31I GGTCTC 2 cut(s) 143, 2975
Eco32I GATATC 1 cut(s) 1341
Eco47I GGWCC 3 cut(s) 82, 1791, 1957
Eco57I CTGAAG 3 cut(s) 80, 1743, 2045
EcoNI CCTNNNNNAGG 1 cut(s) 1530
EcoO109I RGGNCCY 2 cut(s) 1090, 2609
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 2 cut(s) 1118, 1909
EcoRV GATATC 1 cut(s) 1341
EcoT14I CCWWGG 2 cut(s) 1481, 2361
EcoT38I GRGCYC 1 cut(s) 2659
ErhI CCWWGG 2 cut(s) 1481, 2361
Esp3I CGTCTC 1 cut(s) 2992
FaqI GGGAC 1 cut(s) 923
FauI CCCGC 1 cut(s) 1774
FauNDI CATATG 1 cut(s) 1663
FbaI TGATCA 1 cut(s) 1636
FblI GTMKAC 1 cut(s) 2568
Fnu4HI GCNGC 7 cut(s) 183, 1332, 1375, 1547, 1874, 2296, 2958
FokI GGATG 6 cut(s) 553, 560, 1499, 2594, 2621, 2636
FriOI GRGCYC 1 cut(s) 2659
Fsp4HI GCNGC 7 cut(s) 183, 1332, 1375, 1547, 1874, 2296, 2958
FspAI RTGCGCAY 1 cut(s) 552
FspBI CTAG 5 cut(s) 1028, 1094, 1227, 2268, 2903
FspI TGCGCA 2 cut(s) 552, 2294
GlaI GCGC 7 cut(s) 340, 496, 552, 2294, 2811, 3015, 3017
GluI GCNGC 7 cut(s) 183, 1332, 1375, 1547, 1874, 2296, 2958
GsuI CTGGAG 3 cut(s) 867, 1286, 2714
HaeII RGCGCY 2 cut(s) 342, 498
HaeIII GGCC 6 cut(s) 425, 1091, 1295, 1717, 2041, 2611
HapII CCGG 2 cut(s) 14, 45
HgaI GACGC 1 cut(s) 764
HhaI GCGC 7 cut(s) 341, 497, 553, 2295, 2812, 3016, 3018
Hin6I GCGC 7 cut(s) 339, 495, 551, 2293, 2810, 3014, 3016
HinP1I GCGC 7 cut(s) 339, 495, 551, 2293, 2810, 3014, 3016
HincII GTYRAC 4 cut(s) 115, 400, 1224, 2569
HindII GTYRAC 4 cut(s) 115, 400, 1224, 2569
HindIII AAGCTT 3 cut(s) 404, 1808, 2082
HpaII CCGG 2 cut(s) 14, 45
HphI GGTGA 3 cut(s) 79, 1839, 1850
Hpy99I CGWCG 1 cut(s) 110
HpyCH4III ACNGT 8 cut(s) 376, 628, 643, 904, 2184, 2458, 2856, 2863
HpyCH4IV ACGT 4 cut(s) 820, 2379, 2593, 3001
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 272, 550, 692, 2742, 2963, 2972
HpySE526I ACGT 4 cut(s) 820, 2379, 2593, 3001
HspAI GCGC 7 cut(s) 339, 495, 551, 2293, 2810, 3014, 3016
KpnI GGTACC 1 cut(s) 670
Ksp22I TGATCA 1 cut(s) 1636
KspI CCGCGG 1 cut(s) 2961
LguI GCTCTTC 1 cut(s) 807
LmnI GCTCC 4 cut(s) 1571, 1814, 2008, 2344
Lsp1109I GCAGC 6 cut(s) 194, 1343, 1386, 1558, 1885, 2307
LweI GCATC 6 cut(s) 268, 531, 538, 1092, 1926, 2932
MaeI CTAG 5 cut(s) 1028, 1094, 1227, 2268, 2903
MaeII ACGT 4 cut(s) 820, 2379, 2593, 3001
MaeIII GTNAC 5 cut(s) 54, 355, 1359, 1903, 2898
MfeI CAATTG 1 cut(s) 1392
MflI RGATCY 4 cut(s) 988, 1975, 2113, 2448
MhlI GDGCHC 2 cut(s) 1456, 2659
MlyI GAGTC 3 cut(s) 874, 1106, 2752
MmeI TCCRAC 1 cut(s) 88
MroXI GAANNNNTTC 5 cut(s) 628, 1474, 1497, 1584, 2373
MslI CAYNNNNRTG 2 cut(s) 77, 2664
MspA1I CMGCKG 3 cut(s) 887, 1377, 2960
MspI CCGG 2 cut(s) 14, 45
MspR9I CCNGG 4 cut(s) 15, 46, 1120, 1911
MunI CAATTG 1 cut(s) 1392
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 2 cut(s) 1120, 1911
MvnI CGCG 4 cut(s) 1209, 2235, 2960, 3016
MwoI GCNNNNNNNGC 7 cut(s) 188, 272, 550, 692, 2742, 2963, 2972
NciI CCSGG 2 cut(s) 15, 46
NcoI CCATGG 2 cut(s) 1481, 2361
NdeI CATATG 1 cut(s) 1663
NlaIV GGNNCC 5 cut(s) 668, 1716, 1977, 2115, 2359
NmuCI GTSAC 2 cut(s) 54, 355
NsbI TGCGCA 2 cut(s) 552, 2294
NspV TTCGAA 1 cut(s) 622
PagI TCATGA 2 cut(s) 295, 808
PauI GCGCGC 1 cut(s) 3014
PceI AGGCCT 1 cut(s) 2041
PciSI GCTCTTC 1 cut(s) 807
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 5 cut(s) 628, 1474, 1497, 1584, 2373
PflMI CCANNNNNTGG 1 cut(s) 91
PfoI TCCNGGA 1 cut(s) 13
PkrI GCNGC 7 cut(s) 184, 1333, 1376, 1548, 1875, 2297, 2959
PleI GAGTC 3 cut(s) 874, 1105, 2752
PpsI GAGTC 3 cut(s) 874, 1105, 2752
Psp6I CCWGG 2 cut(s) 1118, 1909
PspGI CCWGG 2 cut(s) 1118, 1909
PspN4I GGNNCC 5 cut(s) 668, 1716, 1977, 2115, 2359
PspPI GGNCC 7 cut(s) 82, 1090, 1294, 1715, 1791, 1957, 2609
PstI CTGCAG 1 cut(s) 862
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 4 cut(s) 988, 1975, 2113, 2448
PteI GCGCGC 1 cut(s) 3014
PvuII CAGCTG 2 cut(s) 887, 1377
RsaI GTAC 8 cut(s) 287, 512, 668, 1125, 1621, 1697, 2858, 2884
RsaNI GTAC 8 cut(s) 286, 511, 667, 1124, 1620, 1696, 2857, 2883
RseI CAYNNNNRTG 2 cut(s) 77, 2664
SacII CCGCGG 1 cut(s) 2961
SalI GTCGAC 1 cut(s) 2567
SapI GCTCTTC 1 cut(s) 807
SatI GCNGC 7 cut(s) 183, 1332, 1375, 1547, 1874, 2296, 2958
Sau96I GGNCC 7 cut(s) 82, 1090, 1294, 1715, 1791, 1957, 2609
ScaI AGTACT 1 cut(s) 1697
SchI GAGTC 3 cut(s) 874, 1106, 2752
ScrFI CCNGG 4 cut(s) 15, 46, 1120, 1911
SduI GDGCHC 2 cut(s) 1456, 2659
SfaNI GCATC 6 cut(s) 268, 531, 538, 1092, 1926, 2932
SfcI CTRYAG 6 cut(s) 696, 858, 913, 1158, 2160, 2862
Sfr303I CCGCGG 1 cut(s) 2961
SfuI TTCGAA 1 cut(s) 622
SgrBI CCGCGG 1 cut(s) 2961
SinI GGWCC 3 cut(s) 82, 1791, 1957
SmiMI CAYNNNNRTG 2 cut(s) 77, 2664
SmlI CTYRAG 2 cut(s) 1457, 2760
SmoI CTYRAG 2 cut(s) 1457, 2760
SseBI AGGCCT 1 cut(s) 2041
SsiI CCGC 9 cut(s) 440, 1017, 1781, 2193, 2233, 2431, 2481, 2958, 2960
SspI AATATT 8 cut(s) 1244, 1540, 1559, 1567, 2093, 2149, 2178, 2589
SspMI CTAG 5 cut(s) 1028, 1094, 1227, 2268, 2903
StuI AGGCCT 1 cut(s) 2041
StyD4I CCNGG 4 cut(s) 13, 44, 1118, 1909
StyI CCWWGG 2 cut(s) 1481, 2361
TaaI ACNGT 8 cut(s) 376, 628, 643, 904, 2184, 2458, 2856, 2863
TaiI ACGT 4 cut(s) 823, 2382, 2596, 3004
TatI WGTACW 5 cut(s) 285, 510, 1619, 1695, 2856
TauI GCSGC 1 cut(s) 2960
TseFI GTSAC 2 cut(s) 54, 355
TseI GCWGC 6 cut(s) 182, 1331, 1374, 1546, 1873, 2295
Tsp45I GTSAC 2 cut(s) 54, 355
TspDTI ATGAA 8 cut(s) 823, 825, 1254, 1281, 1503, 2093, 2598, 2676
TspGWI ACGGA 2 cut(s) 1250, 1949
Van91I CCANNNNNTGG 1 cut(s) 91
VpaK11BI GGWCC 3 cut(s) 82, 1791, 1957
XagI CCTNNNNNAGG 1 cut(s) 1530
XapI RAATTY 4 cut(s) 332, 443, 2530, 2550
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 2568
XmnI GAANNNNTTC 5 cut(s) 628, 1474, 1497, 1584, 2373
XspI CTAG 5 cut(s) 1028, 1094, 1227, 2268, 2903
ZrmI AGTACT 1 cut(s) 1697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.