Rh2CG122300

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000246
Physical Location & Seq
Reverse (-)
10845855 .. 10847619
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG122300.1

Sequence Viewer

Length: 1194 bp
ATGGATGACACCGTCGACTGGTCCAATCTTCCAGTCGAACTTTGGCCACTGATCGGAGAAAAGCTCAAGCTCCGCATCGAGTCTCTCCGATTCCGCAGCGTTTGCCATTCATGGCGTTCTTGTTTGCCTCGCTTCCACCCTTCACTTCCACCCAAATTCCCTCTCCCATCCTCCAATGGACCCACCTCCTTCCTCTCCCAAAACACCGTCTACCTCCTCCAGAGCCCCTCCACGTCATCGCTGGCGGGGCCGTCATCGTCTGGAGGTTGGTTGGTCAAGGTCGAAGAGTGCGACAGAATGCTTCTTCGGAATCCTATCACCTCCCGCCGAATCAGGTATTCAAACGACTCGTCGACGTTGTCTCCGGAATGTTTCAATTTGTTGGAGTTTAAAATGATCGAATTAGGGAAATCCTATTTTCTGAGATTCATCCGTGGCTCTGGTTCTGTTGATGTGTTAAAGAAAGTGATTTTAATGCCTCCTGAGATTGTTGATTGTGCAATTCTGGTTATCTGTGATGAAGGAAATTTGGGTTTTGCTAAGAGTGGAGATGAGAAACTCACAAAATTCAGTAATATTGGAGGTTTTGTTTTTGATGATGTATTTGTTTATAAGGGTCAGCCATATGTGGTGGATAGCTTGGGTTGTGTTTTTAGGATTGATGTGTCTTCAAAAGTGATGGTGAAAATTTCGTCGGAATTGGTTGGTTTAGGTGGGAGGAAGCATTTGGTGGAGTCTTGCGGAGAGCTTTATGTGGTCGATTGGTATTTTGATCAAGAACAGAATAAGCAAGAAGCGACTGGTCTTGAGTTAGATTTTTCTTTTTGGGGCTTTCGTCGTCGTGGCAGATACCGGAGATATCATTCTGGTGAACTCAAGGTAGTTGATTTTAAGGTTTACAAGCTGGAACTGTTGGATGGAGAGTTGGGCAGATGGGTTGAAGTGGAAAGCTTGGGTGGTCAAGCGTTTTTCTTGGCTATAGATTGCTCTTTCTCTGTATTGGCTGAAGAATTAGCTGGGTGCAAAGGTAATTGCATTTACTTCTCAGATGAAAATGACGTTAATCTTGCTCTTAGAGAAGTAACTAGACCAGAGAGTTTTGTGTTCAACTTAGAGGACCATAGCATTCAGAAGTTGGGATCCTCCCCAGGCGGTTCCGGATCCCAGATATATTGGCCACCTCGCTCCAATTGA

Protein Analysis

397

Amino Acids

44.74

Weight (kDa)

5.73

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 66 - 354 5.2e-41 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44080 AT1G57906 AT1G65735 AT1G65740 AT1G65760 AT1G65770 AT2G17690 AT2G26160 AT3G25750 AT3G25750 AT4G35733
fragaria_vesca FvH4_1g10810 FvH4_1g10830 FvH4_1g10840 FvH4_1g10840 FvH4_1g10840 FvH4_1g10860 FvH4_1g10880
malus_domestica MD02G1122000.v1.1 MD15G1235700.v1.1 MD15G1235900.v1.1
prunus_persica Prupe.7G177000_v2.0.a1 Prupe.7G177100_v2.0.a1 Prupe.7G177200_v2.0.a1 Prupe.7G177300_v2.0.a1 Prupe.7G177400_v2.0.a1 Prupe.7G177400_v2.0.a1
pyrus_communis pycom02g09500 pycom15g20990 pycom15g21000
rosa_chinensis RchiOBHm_Chr1g0325601 RchiOBHm_Chr2g0098071 RchiOBHm_Chr2g0098081 RchiOBHm_Chr2g0098091 RchiOBHm_Chr2g0098121 RchiOBHm_Chr2g0098131 RchiOBHm_Chr2g0098151 RchiOBHm_Chr2g0134711 RchiOBHm_Chr3g0456141 RchiOBHm_Chr4g0394811 RchiOBHm_Chr4g0416561 RchiOBHm_Chr5g0014321
rosa_laevigata RLG00000001178 RLG00000016753 RLG00000016754 RLG00000016755 RLG00000016756 RLG00000016757 RLG00000016758 RLG00000016760 RLG00000016761 RLG00000016762 RLG00000016763
rosa_multiflora Rmu_co8307469.1_g000001 Rmu_sc0000427.1_g000002 Rmu_sc0002880.1_g000004 Rmu_sc0002880.1_g000007 Rmu_sc0009178.1_g000019 Rmu_sc0013919.1_g000002 Rmu_sc0013919.1_g000003 Rmu_sc0014278.1_g000001 Rmu_sc0031755.1_g000001 Rmu_sc0031755.1_g000002 Rmu_sc0039043.1_g000001 Rmu_sc0039432.1_g000001
rosa_roxburghii Rroxscaffold_2G00144260 Rroxscaffold_2G00144270 Rroxscaffold_2G00144290 Rroxscaffold_2G00144310 Rroxscaffold_2G00144320 Rroxscaffold_2G00144340 Rroxscaffold_3G00238070
rosa_rugosa Rorug02G0069800 Rorug02G0069900 Rorug02G0070000 Rorug02G0070100 Rorug02G0070200 Rorug02G0070500 Rorug02G0070500 Rorug05G0053500 Rorug05G0482400 Rorug07G0244200
rosa_samantha Rh1DG210300 Rh2AG117100 Rh2AG117200 Rh2AG117300 Rh2AG117500 Rh2AG117700 Rh2AG117800 Rh2AG563300 Rh2BG119900 Rh2BG120000 Rh2BG120200 Rh2BG120300 Rh2BG120400 Rh2BG120600 Rh2BG120700 Rh2BG377200 Rh2CG076100 Rh2CG076200 Rh2CG121600 Rh2CG121700 Rh2CG121900 Rh2CG122000 Rh2CG122100 Rh2CG122200 Rh2CG122300 Rh2DG121600 Rh2DG121700 Rh2DG122000 Rh2DG122100 Rh2DG122300 Rh3AG138900 Rh3BG074100 Rh3CG073500 Rh3DG298600 Rh4CG222800 Rh4CG222900 Rh5CG120400 Rh6BG018400 Rh6BG124600 Rh6CG123200 Rh6CG123300 Rh7AG342600 Rh7AG342700 Rh7BG352100 Rh7CG128700 Rh7CG360200 Rh7CG360300
rosa_wichuraiana Rw2G009150 Rw2G009160 Rw2G009170 Rw2G009200 Rw4G016710 Rw5G035380 Rw6G043260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AccI GTMKAC 3 cut(s) 15, 210, 353
AccIII TCCGGA 2 cut(s) 364, 1157
AciI CCGC 6 cut(s) 73, 94, 245, 325, 741, 1152
AclWI GGATC 4 cut(s) 1134, 1147, 1155, 1168
AcoI YGGCCR 2 cut(s) 44, 1175
AcsI RAATTY 4 cut(s) 155, 526, 566, 687
AcuI CTGAAG 1 cut(s) 1026
AfiI CCNNNNNNNGG 2 cut(s) 18, 53
AgsI TTSAA 5 cut(s) 342, 376, 672, 941, 1108
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 1 cut(s) 1147
AluBI AGCT 7 cut(s) 64, 70, 639, 748, 904, 951, 1016
AluI AGCT 7 cut(s) 64, 70, 639, 748, 904, 951, 1016
Alw26I GTCTC 2 cut(s) 87, 366
AlwI GGATC 4 cut(s) 1134, 1147, 1155, 1168
Aor13HI TCCGGA 2 cut(s) 364, 1157
AoxI GGCC 3 cut(s) 44, 248, 1175
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 4 cut(s) 155, 526, 566, 687
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AspS9I GGNCC 4 cut(s) 21, 179, 248, 1117
AsuHPI GGTGA 3 cut(s) 310, 694, 881
AvaII GGWCC 3 cut(s) 21, 179, 1117
BalI TGGCCA 2 cut(s) 46, 1177
BamHI GGATCC 2 cut(s) 1139, 1160
BanII GRGCYC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 660
BbvI GCAGC 1 cut(s) 108
BccI CCATC 4 cut(s) 175, 673, 911, 927
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 1149
BclI TGATCA 1 cut(s) 772
BcoDI GTCTC 2 cut(s) 87, 366
BfaI CTAG 1 cut(s) 1086
BfmI CTRYAG 1 cut(s) 978
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme1390I CCNGG 1 cut(s) 1149
Bme18I GGWCC 3 cut(s) 21, 179, 1117
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 4 cut(s) 21, 179, 248, 1117
BmiI GGNNCC 5 cut(s) 181, 249, 1141, 1156, 1162
BmrFI CCNGG 1 cut(s) 1149
BmsI GCATC 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 660
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpmI CTGGAG 2 cut(s) 203, 282
BpuEI CTTGAG 3 cut(s) 50, 827, 860
BsaJI CCNNGG 2 cut(s) 433, 1147
BsaWI WCCGGW 3 cut(s) 364, 852, 1157
BsaXI ACNNNNNCTCC 4 cut(s) 346, 376, 573, 603
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 53
Bse1I ACTGG 3 cut(s) 23, 32, 805
BseAI TCCGGA 2 cut(s) 364, 1157
BseBI CCWGG 1 cut(s) 1149
BseDI CCNNGG 2 cut(s) 433, 1147
BseGI GGATG 4 cut(s) 10, 167, 429, 922
BseLI CCNNNNNNNGG 2 cut(s) 18, 53
BseMII CTCAG 3 cut(s) 413, 474, 1059
BseNI ACTGG 3 cut(s) 23, 32, 805
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 1016
BshFI GGCC 3 cut(s) 46, 250, 1177
BsiSI CCGG 3 cut(s) 365, 853, 1158
BslI CCNNNNNNNGG 2 cut(s) 18, 53
BsmAI GTCTC 2 cut(s) 87, 366
BsmI GAATGC 2 cut(s) 303, 1125
BsnI GGCC 3 cut(s) 46, 250, 1177
Bsp1286I GDGCHC 1 cut(s) 227
Bsp13I TCCGGA 2 cut(s) 364, 1157
Bsp143I GATC 5 cut(s) 51, 396, 772, 1139, 1160
BspACI CCGC 6 cut(s) 73, 94, 245, 325, 741, 1152
BspANI GGCC 3 cut(s) 46, 250, 1177
BspCNI CTCAG 3 cut(s) 414, 475, 1058
BspEI TCCGGA 2 cut(s) 364, 1157
BspLI GGNNCC 5 cut(s) 181, 249, 1141, 1156, 1162
BspPI GGATC 4 cut(s) 1134, 1147, 1155, 1168
BsrI ACTGG 3 cut(s) 23, 32, 805
BssECI CCNNGG 2 cut(s) 433, 1147
BssMI GATC 5 cut(s) 51, 396, 772, 1139, 1160
Bst2UI CCWGG 1 cut(s) 1149
Bst4CI ACNGT 3 cut(s) 13, 208, 912
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 6 cut(s) 422, 483, 540, 1045, 1073, 1111
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 4 cut(s) 10, 167, 429, 922
BstKTI GATC 5 cut(s) 54, 399, 775, 1142, 1163
BstMAI GTCTC 2 cut(s) 87, 366
BstMBI GATC 5 cut(s) 51, 396, 772, 1139, 1160
BstMWI GCNNNNNNNGC 2 cut(s) 102, 247
BstNI CCWGG 1 cut(s) 1149
BstSCI CCNGG 1 cut(s) 1147
BstSFI CTRYAG 1 cut(s) 978
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 660
BstX2I RGATCY 2 cut(s) 1139, 1160
BstYI RGATCY 2 cut(s) 1139, 1160
BsuRI GGCC 3 cut(s) 46, 250, 1177
BtgI CCRYGG 1 cut(s) 433
BtgZI GCGATG 1 cut(s) 222
BtrI CACGTC 1 cut(s) 234
BtsCI GGATG 4 cut(s) 10, 167, 429, 922
BtsIMutI CAGTG 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 243
Cfr13I GGNCC 4 cut(s) 21, 179, 248, 1117
CviAII CATG 1 cut(s) 111
DdeI CTNAG 6 cut(s) 422, 483, 540, 1045, 1073, 1111
DpnI GATC 5 cut(s) 53, 398, 774, 1141, 1162
DpnII GATC 5 cut(s) 51, 396, 772, 1139, 1160
DraI TTTAAA 1 cut(s) 391
EaeI YGGCCR 2 cut(s) 44, 1175
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 227
Eco32I GATATC 1 cut(s) 860
Eco47I GGWCC 3 cut(s) 21, 179, 1117
Eco57I CTGAAG 1 cut(s) 1026
EcoRII CCWGG 1 cut(s) 1147
EcoRV GATATC 1 cut(s) 860
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 1 cut(s) 114
FaiI YATR 8 cut(s) 112, 612, 625, 627, 753, 980, 1122, 1171
FatI CATG 1 cut(s) 110
FauI CCCGC 2 cut(s) 238, 332
FauNDI CATATG 1 cut(s) 625
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 3 cut(s) 15, 210, 353
Fnu4HI GCNGC 1 cut(s) 97
FokI GGATG 4 cut(s) 17, 154, 416, 929
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 97
FspBI CTAG 1 cut(s) 1086
GluI GCNGC 1 cut(s) 97
GsaI CCCAGC 1 cut(s) 1020
GsuI CTGGAG 2 cut(s) 203, 282
HaeIII GGCC 3 cut(s) 46, 250, 1177
HapII CCGG 3 cut(s) 365, 853, 1158
Hin1II CATG 1 cut(s) 114
HincII GTYRAC 2 cut(s) 16, 354
HindII GTYRAC 2 cut(s) 16, 354
HindIII AAGCTT 1 cut(s) 949
HinfI GANTC 7 cut(s) 80, 90, 310, 330, 347, 426, 734
HpaII CCGG 3 cut(s) 365, 853, 1158
HphI GGTGA 3 cut(s) 310, 694, 881
Hpy166II GTNNAC 5 cut(s) 16, 211, 354, 872, 898
Hpy188I TCNGA 7 cut(s) 56, 89, 309, 423, 697, 1048, 1131
Hpy188III TCNNGA 7 cut(s) 220, 261, 365, 482, 776, 806, 1158
Hpy8I GTNNAC 5 cut(s) 16, 211, 354, 872, 898
Hpy99I CGWCG 6 cut(s) 17, 355, 358, 697, 840, 843
HpyAV CCTTC 3 cut(s) 150, 199, 515
HpyCH4III ACNGT 3 cut(s) 13, 208, 912
HpyCH4IV ACGT 3 cut(s) 233, 356, 1059
HpyCH4V TGCA 3 cut(s) 500, 1023, 1035
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 247
HpyF3I CTNAG 6 cut(s) 422, 483, 540, 1045, 1073, 1111
HpySE526I ACGT 3 cut(s) 233, 356, 1059
Hsp92II CATG 1 cut(s) 114
Kpn2I TCCGGA 2 cut(s) 364, 1157
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 5 cut(s) 51, 396, 772, 1139, 1160
LmnI GCTCC 2 cut(s) 75, 1190
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 84
MaeI CTAG 1 cut(s) 1086
MaeII ACGT 3 cut(s) 233, 356, 1059
MaeIII GTNAC 1 cut(s) 1081
MalI GATC 5 cut(s) 53, 398, 774, 1141, 1162
MboI GATC 5 cut(s) 51, 396, 772, 1139, 1160
MboII GAAGA 5 cut(s) 20, 296, 296, 660, 1019
MfeI CAATTG 1 cut(s) 1189
MflI RGATCY 2 cut(s) 1139, 1160
MhlI GDGCHC 1 cut(s) 227
MlsI TGGCCA 2 cut(s) 46, 1177
MluNI TGGCCA 2 cut(s) 46, 1177
MlyI GAGTC 3 cut(s) 89, 341, 743
MmeI TCCRAC 3 cut(s) 363, 675, 894
Mox20I TGGCCA 2 cut(s) 46, 1177
MroI TCCGGA 2 cut(s) 364, 1157
MscI TGGCCA 2 cut(s) 46, 1177
MseI TTAA 5 cut(s) 390, 458, 473, 891, 1062
MslI CAYNNNNRTG 1 cut(s) 867
Msp20I TGGCCA 2 cut(s) 46, 1177
MspI CCGG 3 cut(s) 365, 853, 1158
MspR9I CCNGG 1 cut(s) 1149
MunI CAATTG 1 cut(s) 1189
Mva1269I GAATGC 2 cut(s) 303, 1125
MvaI CCWGG 1 cut(s) 1149
MwoI GCNNNNNNNGC 2 cut(s) 102, 247
NdeI CATATG 1 cut(s) 625
NdeII GATC 5 cut(s) 51, 396, 772, 1139, 1160
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 5 cut(s) 181, 249, 1141, 1156, 1162
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PctI GAATGC 2 cut(s) 303, 1125
PfeI GAWTC 4 cut(s) 90, 310, 330, 426
PflFI GACNNNGTC 2 cut(s) 11, 358
PkrI GCNGC 1 cut(s) 98
PleI GAGTC 3 cut(s) 88, 341, 742
PpsI GAGTC 3 cut(s) 88, 341, 742
PsiI TTATAA 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 1147
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 1 cut(s) 1147
PspN4I GGNNCC 5 cut(s) 181, 249, 1141, 1156, 1162
PspPI GGNCC 4 cut(s) 21, 179, 248, 1117
PsuI RGATCY 2 cut(s) 1139, 1160
PsyI GACNNNGTC 2 cut(s) 11, 358
RseI CAYNNNNRTG 1 cut(s) 867
SalI GTCGAC 2 cut(s) 14, 352
SaqAI TTAA 5 cut(s) 390, 458, 473, 891, 1062
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 5 cut(s) 51, 396, 772, 1139, 1160
Sau96I GGNCC 4 cut(s) 21, 179, 248, 1117
SchI GAGTC 3 cut(s) 89, 341, 743
ScrFI CCNGG 1 cut(s) 1149
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 1 cut(s) 978
SgrDI CGTCGACG 1 cut(s) 352
SinI GGWCC 3 cut(s) 21, 179, 1117
SmiMI CAYNNNNRTG 1 cut(s) 867
SmlI CTYRAG 3 cut(s) 65, 806, 875
SmoI CTYRAG 3 cut(s) 65, 806, 875
SsiI CCGC 6 cut(s) 73, 94, 245, 325, 741, 1152
SspI AATATT 1 cut(s) 577
SspMI CTAG 1 cut(s) 1086
StyD4I CCNGG 1 cut(s) 1147
TaaI ACNGT 3 cut(s) 13, 208, 912
TaiI ACGT 3 cut(s) 236, 359, 1062
TaqI TCGA 7 cut(s) 15, 36, 78, 282, 353, 399, 759
TfiI GAWTC 4 cut(s) 90, 310, 330, 426
Tru1I TTAA 5 cut(s) 390, 458, 473, 891, 1062
Tru9I TTAA 5 cut(s) 390, 458, 473, 891, 1062
TscAI CASTG 1 cut(s) 54
TseI GCWGC 1 cut(s) 96
TspDTI ATGAA 4 cut(s) 99, 418, 534, 1065
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 1 cut(s) 54
Tth111I GACNNNGTC 2 cut(s) 11, 358
VpaK11BI GGWCC 3 cut(s) 21, 179, 1117
XapI RAATTY 4 cut(s) 155, 526, 566, 687
XcmI CCANNNNNNNNNTGG 2 cut(s) 39, 238
XmiI GTMKAC 3 cut(s) 15, 210, 353
XspI CTAG 1 cut(s) 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.