Rroxscaffold_2G00144270

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000246
Physical Location & Seq
Forward (+)
82184361 .. 82185563
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144270.1

Sequence Viewer

Length: 1203 bp
ATGGATGACACCGTCGACTGGTCCAATCTTCCAGTCGAACTTTGGCCACTGATCGGAGAAAAGCTCAAGCTCCGCATCGAGAATCTCCGATTCCGCAGCGTTTGCCATTCATGGCGTTCTTGTTTGCCTCGCTTCCACCCTTCACTTCCACCCAAATTCCCTCTCCCATCCTCCAATGGACCCACCTCCTTCCTCTCCCTAAACACCGTCTACCTCCTACAGAGTCCCTGCACGTCATCACTGGCGGCGCAGTCTTCGTCTGGAGGTTGGTTGGTCAAGGTCGAGGAGTGCGACAGAATGCTTCTTCGGAATCCTATCACCTCCCGCCGAATCAGGTATTCAAACGACTCGTCGTTGTCTCCGGAATGTTTCAATTTGTTGGAGTTTAAAATGATCGAAATAGGGAAATCCTATTTTCTGAGATACATCCGTGGCTCTGGTTCTGTTGATGTGTTAAAGAAAGTGGTTTTAATGCCTCCTGAGATTGCTGATTGTGCAATTCTGTTTATCTGTGATAAAGGAAATTTGGGTTTTGCTAAGAGTGGAGATGAGAAACTCACAAAATTCAGTAATGAAGGTTTTGTTTTTGATGATGTTATTGTTTATAAGGGTCAGCCATACGTGGTGGATAGCTTGGGTTGTGTTTTTAGGATTGATGTGTCTTCAAAAGTGATGGTGAAAATTTCGTCGGAATTGGTTGGTTTAGGTGGGAGGAAGCATTTGGTGGAGTCTTGCGGAGAGCTTTATGTGGTCGATAGGTATTTTGATCAAGAACAAAATAAGCAAGAAGCGACTGGTCTTGGGTTGGATTTTTCTTTTTGGGGCTTTCGTCGTCGTCGTGGCAGATACCGGAGATATCATTCTGGTGAACTCATGGCAGTTGATTTTAAGGTTTACAAGCTGGAACTGTTGGATGGAGAGTTGGGCAGATGGGTTGAAGTGGAAAGCTTGGGTGGTCAAGCGTTTTTCTTGGCTATAGATTGCTCTTTCTCTGTATTGGCTGAAGAATTAGCTGGGTGCAAAGGGAATTGCATATACTTCTCAGATGAAAATGACGTTAATCTTGCTCTTAGAGAAGTAATTAGACCAGAGAGTTTTGTGTTCAACTTAGAGGACCATAGCATTCAGAAGTTGGGATCTTCCCCAGGCGGTTCCCAGATATATTGGCCATCGCCAATTGAGCTCGGCTCCCCGTGTTCGTAA

Protein Analysis

400

Amino Acids

45.07

Weight (kDa)

5.89

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 67 - 353 4.1e-43 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44080 AT1G57906 AT1G65735 AT1G65740 AT1G65760 AT1G65770 AT2G17690 AT2G26160 AT3G25750 AT3G25750 AT4G35733
fragaria_vesca FvH4_1g10810 FvH4_1g10830 FvH4_1g10840 FvH4_1g10840 FvH4_1g10840 FvH4_1g10860 FvH4_1g10880
malus_domestica MD02G1122000.v1.1 MD15G1235700.v1.1 MD15G1235900.v1.1
prunus_persica Prupe.7G177000_v2.0.a1 Prupe.7G177100_v2.0.a1 Prupe.7G177200_v2.0.a1 Prupe.7G177300_v2.0.a1 Prupe.7G177400_v2.0.a1 Prupe.7G177400_v2.0.a1
pyrus_communis pycom02g09500 pycom15g20990 pycom15g21000
rosa_chinensis RchiOBHm_Chr1g0325601 RchiOBHm_Chr2g0098071 RchiOBHm_Chr2g0098081 RchiOBHm_Chr2g0098091 RchiOBHm_Chr2g0098121 RchiOBHm_Chr2g0098131 RchiOBHm_Chr2g0098151 RchiOBHm_Chr2g0134711 RchiOBHm_Chr3g0456141 RchiOBHm_Chr4g0394811 RchiOBHm_Chr4g0416561 RchiOBHm_Chr5g0014321
rosa_laevigata RLG00000001178 RLG00000016753 RLG00000016754 RLG00000016755 RLG00000016756 RLG00000016757 RLG00000016758 RLG00000016760 RLG00000016761 RLG00000016762 RLG00000016763
rosa_multiflora Rmu_co8307469.1_g000001 Rmu_sc0000427.1_g000002 Rmu_sc0002880.1_g000004 Rmu_sc0002880.1_g000007 Rmu_sc0009178.1_g000019 Rmu_sc0013919.1_g000002 Rmu_sc0013919.1_g000003 Rmu_sc0014278.1_g000001 Rmu_sc0031755.1_g000001 Rmu_sc0031755.1_g000002 Rmu_sc0039043.1_g000001 Rmu_sc0039432.1_g000001
rosa_roxburghii Rroxscaffold_2G00144260 Rroxscaffold_2G00144270 Rroxscaffold_2G00144290 Rroxscaffold_2G00144310 Rroxscaffold_2G00144320 Rroxscaffold_2G00144340 Rroxscaffold_3G00238070
rosa_rugosa Rorug02G0069800 Rorug02G0069900 Rorug02G0070000 Rorug02G0070100 Rorug02G0070200 Rorug02G0070500 Rorug02G0070500 Rorug05G0053500 Rorug05G0482400 Rorug07G0244200
rosa_samantha Rh1DG210300 Rh2AG117100 Rh2AG117200 Rh2AG117300 Rh2AG117500 Rh2AG117700 Rh2AG117800 Rh2AG563300 Rh2BG119900 Rh2BG120000 Rh2BG120200 Rh2BG120300 Rh2BG120400 Rh2BG120600 Rh2BG120700 Rh2BG377200 Rh2CG076100 Rh2CG076200 Rh2CG121600 Rh2CG121700 Rh2CG121900 Rh2CG122000 Rh2CG122100 Rh2CG122200 Rh2CG122300 Rh2DG121600 Rh2DG121700 Rh2DG122000 Rh2DG122100 Rh2DG122300 Rh3AG138900 Rh3BG074100 Rh3CG073500 Rh3DG298600 Rh4CG222800 Rh4CG222900 Rh5CG120400 Rh6BG018400 Rh6BG124600 Rh6CG123200 Rh6CG123300 Rh7AG342600 Rh7AG342700 Rh7BG352100 Rh7CG128700 Rh7CG360200 Rh7CG360300
rosa_wichuraiana Rw2G009150 Rw2G009160 Rw2G009170 Rw2G009200 Rw4G016710 Rw5G035380 Rw6G043260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AccI GTMKAC 2 cut(s) 15, 210
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 6 cut(s) 73, 94, 245, 325, 735, 1149
AclWI GGATC 1 cut(s) 1144
AcoI YGGCCR 2 cut(s) 44, 1166
AcsI RAATTY 4 cut(s) 155, 523, 563, 681
AcuI CTGAAG 1 cut(s) 1023
AfiI CCNNNNNNNGG 2 cut(s) 18, 53
AgsI TTSAA 5 cut(s) 342, 373, 666, 938, 1105
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 1 cut(s) 1144
AluBI AGCT 8 cut(s) 64, 70, 633, 742, 901, 948, 1013, 1183
AluI AGCT 8 cut(s) 64, 70, 633, 742, 901, 948, 1013, 1183
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 1 cut(s) 1144
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 2 cut(s) 44, 1166
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 4 cut(s) 155, 523, 563, 681
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 3 cut(s) 21, 179, 1114
AsuHPI GGTGA 3 cut(s) 310, 688, 878
AvaII GGWCC 3 cut(s) 21, 179, 1114
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BalI TGGCCA 2 cut(s) 46, 1168
BanII GRGCYC 1 cut(s) 1185
BbsI GAAGAC 2 cut(s) 246, 654
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 1 cut(s) 108
BccI CCATC 5 cut(s) 175, 667, 908, 924, 1177
BciT130I CCWGG 1 cut(s) 1146
BclI TGATCA 1 cut(s) 766
BcoDI GTCTC 1 cut(s) 363
BfmI CTRYAG 2 cut(s) 218, 975
BisI GCNGC 2 cut(s) 97, 246
BlsI GCNGC 2 cut(s) 98, 247
Bme1390I CCNGG 1 cut(s) 1146
Bme18I GGWCC 3 cut(s) 21, 179, 1114
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 3 cut(s) 21, 179, 1114
BmiI GGNNCC 3 cut(s) 181, 1153, 1189
BmrFI CCNGG 1 cut(s) 1146
BmsI GCATC 1 cut(s) 84
BpiI GAAGAC 2 cut(s) 246, 654
BplI GAGNNNNNCTC 3 cut(s) 48, 80, 1172
BpmI CTGGAG 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 50
BsaAI YACGTR 1 cut(s) 622
BsaJI CCNNGG 2 cut(s) 430, 1144
BsaWI WCCGGW 2 cut(s) 361, 849
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 53
Bse1I ACTGG 4 cut(s) 23, 32, 246, 799
BseAI TCCGGA 1 cut(s) 361
BseBI CCWGG 1 cut(s) 1146
BseDI CCNNGG 2 cut(s) 430, 1144
BseGI GGATG 4 cut(s) 10, 167, 426, 919
BseLI CCNNNNNNNGG 2 cut(s) 18, 53
BseMII CTCAG 3 cut(s) 410, 471, 1056
BseNI ACTGG 4 cut(s) 23, 32, 246, 799
BseRI GAGGAG 1 cut(s) 299
BseXI GCAGC 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 1013
BsgI GTGCAG 1 cut(s) 214
BshFI GGCC 2 cut(s) 46, 1168
BsiHKAI GWGCWC 1 cut(s) 1185
BsiSI CCGG 2 cut(s) 362, 850
BslFI GGGAC 1 cut(s) 210
BslI CCNNNNNNNGG 2 cut(s) 18, 53
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 210
BsmI GAATGC 2 cut(s) 303, 1122
BsnI GGCC 2 cut(s) 46, 1168
Bsp1286I GDGCHC 1 cut(s) 1185
Bsp13I TCCGGA 1 cut(s) 361
Bsp143I GATC 4 cut(s) 51, 393, 766, 1136
BspACI CCGC 6 cut(s) 73, 94, 245, 325, 735, 1149
BspANI GGCC 2 cut(s) 46, 1168
BspCNI CTCAG 3 cut(s) 411, 472, 1055
BspEI TCCGGA 1 cut(s) 361
BspLI GGNNCC 3 cut(s) 181, 1153, 1189
BspPI GGATC 1 cut(s) 1144
BsrI ACTGG 4 cut(s) 23, 32, 246, 799
BssECI CCNNGG 2 cut(s) 430, 1144
BssMI GATC 4 cut(s) 51, 393, 766, 1136
Bst2UI CCWGG 1 cut(s) 1146
Bst4CI ACNGT 3 cut(s) 13, 208, 909
BstAPI GCANNNNNTGC 1 cut(s) 102
BstBAI YACGTR 1 cut(s) 622
BstDEI CTNAG 6 cut(s) 419, 480, 537, 1042, 1070, 1108
BstDSI CCRYGG 1 cut(s) 430
BstF5I GGATG 4 cut(s) 10, 167, 426, 919
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 4 cut(s) 54, 396, 769, 1139
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 4 cut(s) 51, 393, 766, 1136
BstMWI GCNNNNNNNGC 3 cut(s) 102, 494, 1180
BstNI CCWGG 1 cut(s) 1146
BstSCI CCNGG 1 cut(s) 1144
BstSFI CTRYAG 2 cut(s) 218, 975
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 2 cut(s) 246, 654
BstX2I RGATCY 1 cut(s) 1136
BstYI RGATCY 1 cut(s) 1136
BsuRI GGCC 2 cut(s) 46, 1168
BtgI CCRYGG 1 cut(s) 430
BtgZI GCGATG 1 cut(s) 1155
BtrI CACGTC 1 cut(s) 234
BtsCI GGATG 4 cut(s) 10, 167, 426, 919
BtsIMutI CAGTG 2 cut(s) 47, 239
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 3 cut(s) 21, 179, 1114
CviAII CATG 2 cut(s) 111, 874
DdeI CTNAG 6 cut(s) 419, 480, 537, 1042, 1070, 1108
DpnI GATC 4 cut(s) 53, 395, 768, 1138
DpnII GATC 4 cut(s) 51, 393, 766, 1136
DraI TTTAAA 1 cut(s) 388
EaeI YGGCCR 2 cut(s) 44, 1166
Ecl136II GAGCTC 1 cut(s) 1183
Eco24I GRGCYC 1 cut(s) 1185
Eco32I GATATC 1 cut(s) 857
Eco47I GGWCC 3 cut(s) 21, 179, 1114
Eco53kI GAGCTC 1 cut(s) 1183
Eco57I CTGAAG 1 cut(s) 1023
EcoICRI GAGCTC 1 cut(s) 1183
EcoRII CCWGG 1 cut(s) 1144
EcoRV GATATC 1 cut(s) 857
EcoT38I GRGCYC 1 cut(s) 1185
FaeI CATG 2 cut(s) 114, 877
FaqI GGGAC 1 cut(s) 210
FatI CATG 2 cut(s) 110, 873
FauI CCCGC 1 cut(s) 332
FbaI TGATCA 1 cut(s) 766
FblI GTMKAC 2 cut(s) 15, 210
Fnu4HI GCNGC 2 cut(s) 97, 246
FokI GGATG 4 cut(s) 17, 154, 413, 926
FriOI GRGCYC 1 cut(s) 1185
Fsp4HI GCNGC 2 cut(s) 97, 246
GlaI GCGC 1 cut(s) 249
GluI GCNGC 2 cut(s) 97, 246
GsaI CCCAGC 1 cut(s) 1017
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 2 cut(s) 46, 1168
HapII CCGG 2 cut(s) 362, 850
HhaI GCGC 1 cut(s) 250
Hin1II CATG 2 cut(s) 114, 877
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 946
HinfI GANTC 7 cut(s) 82, 90, 223, 310, 330, 347, 728
HpaII CCGG 2 cut(s) 362, 850
HphI GGTGA 3 cut(s) 310, 688, 878
Hpy166II GTNNAC 4 cut(s) 16, 211, 869, 895
Hpy188I TCNGA 7 cut(s) 56, 89, 309, 420, 691, 1045, 1128
Hpy188III TCNNGA 5 cut(s) 79, 261, 362, 479, 770
Hpy8I GTNNAC 4 cut(s) 16, 211, 869, 895
Hpy99I CGWCG 6 cut(s) 17, 355, 691, 834, 837, 840
HpyAV CCTTC 3 cut(s) 150, 199, 569
HpyCH4III ACNGT 3 cut(s) 13, 208, 909
HpyCH4IV ACGT 3 cut(s) 233, 621, 1056
HpyCH4V TGCA 4 cut(s) 231, 497, 1020, 1032
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 494, 1180
HpyF3I CTNAG 6 cut(s) 419, 480, 537, 1042, 1070, 1108
HpySE526I ACGT 3 cut(s) 233, 621, 1056
Hsp92II CATG 2 cut(s) 114, 877
HspAI GCGC 1 cut(s) 248
Kpn2I TCCGGA 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 4 cut(s) 51, 393, 766, 1136
LmnI GCTCC 2 cut(s) 75, 1193
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 84
MaeII ACGT 3 cut(s) 233, 621, 1056
MalI GATC 4 cut(s) 53, 395, 768, 1138
MboI GATC 4 cut(s) 51, 393, 766, 1136
MboII GAAGA 6 cut(s) 20, 246, 296, 654, 1016, 1131
MfeI CAATTG 1 cut(s) 1176
MflI RGATCY 1 cut(s) 1136
MhlI GDGCHC 1 cut(s) 1185
MlsI TGGCCA 2 cut(s) 46, 1168
MluNI TGGCCA 2 cut(s) 46, 1168
MlyI GAGTC 3 cut(s) 232, 341, 737
MmeI TCCRAC 4 cut(s) 360, 669, 786, 891
Mox20I TGGCCA 2 cut(s) 46, 1168
MroI TCCGGA 1 cut(s) 361
MscI TGGCCA 2 cut(s) 46, 1168
MseI TTAA 5 cut(s) 387, 455, 470, 888, 1059
MslI CAYNNNNRTG 1 cut(s) 864
Msp20I TGGCCA 2 cut(s) 46, 1168
MspI CCGG 2 cut(s) 362, 850
MspR9I CCNGG 1 cut(s) 1146
MunI CAATTG 1 cut(s) 1176
Mva1269I GAATGC 2 cut(s) 303, 1122
MvaI CCWGG 1 cut(s) 1146
MwoI GCNNNNNNNGC 3 cut(s) 102, 494, 1180
NdeII GATC 4 cut(s) 51, 393, 766, 1136
NlaIII CATG 2 cut(s) 114, 877
NlaIV GGNNCC 3 cut(s) 181, 1153, 1189
NmeAIII GCCGAG 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 835
PctI GAATGC 2 cut(s) 303, 1122
PfeI GAWTC 4 cut(s) 82, 90, 310, 330
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 98, 247
PleI GAGTC 3 cut(s) 231, 341, 736
PpsI GAGTC 3 cut(s) 231, 341, 736
Ppu21I YACGTR 1 cut(s) 622
PsiI TTATAA 1 cut(s) 606
Psp124BI GAGCTC 1 cut(s) 1185
Psp6I CCWGG 1 cut(s) 1144
PspFI CCCAGC 1 cut(s) 1013
PspGI CCWGG 1 cut(s) 1144
PspN4I GGNNCC 3 cut(s) 181, 1153, 1189
PspPI GGNCC 3 cut(s) 21, 179, 1114
PsuI RGATCY 1 cut(s) 1136
PsyI GACNNNGTC 1 cut(s) 11
RseI CAYNNNNRTG 1 cut(s) 864
SacI GAGCTC 1 cut(s) 1185
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 5 cut(s) 387, 455, 470, 888, 1059
SatI GCNGC 2 cut(s) 97, 246
Sau3AI GATC 4 cut(s) 51, 393, 766, 1136
Sau96I GGNCC 3 cut(s) 21, 179, 1114
SchI GAGTC 3 cut(s) 232, 341, 737
ScrFI CCNGG 1 cut(s) 1146
SduI GDGCHC 1 cut(s) 1185
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 2 cut(s) 218, 975
SinI GGWCC 3 cut(s) 21, 179, 1114
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
SsiI CCGC 6 cut(s) 73, 94, 245, 325, 735, 1149
SstI GAGCTC 1 cut(s) 1185
StyD4I CCNGG 1 cut(s) 1144
TaaI ACNGT 3 cut(s) 13, 208, 909
TaiI ACGT 3 cut(s) 236, 624, 1059
TaqI TCGA 6 cut(s) 15, 36, 78, 282, 396, 753
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 4 cut(s) 82, 90, 310, 330
Tru1I TTAA 5 cut(s) 387, 455, 470, 888, 1059
Tru9I TTAA 5 cut(s) 387, 455, 470, 888, 1059
TscAI CASTG 2 cut(s) 54, 246
TseI GCWGC 1 cut(s) 96
TspDTI ATGAA 3 cut(s) 99, 588, 1062
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 2 cut(s) 54, 246
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 3 cut(s) 21, 179, 1114
XapI RAATTY 4 cut(s) 155, 523, 563, 681
XcmI CCANNNNNNNNNTGG 1 cut(s) 39
XmiI GTMKAC 2 cut(s) 15, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.