RLG00000016761

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000246
Physical Location & Seq
Reverse (-)
9803229 .. 9804440
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016761

Sequence Viewer

Length: 1212 bp
ATGGATGACACCGTCGACTGGTCCAATCTTCCAGTCGAACTCTCGCCACTGATCGGAGAAAAGCTCAAGCTCCGCATCGACTCTCTCCGATTCCGCAGCGTTTGCCATTCATGGCGTTCTTGTTTGCCTCGCTTCCACCCTTCACTTCCACCCAAATTCCCTCTCCCATCCTCCAATGGACCCACCTCCTTCCTCTCCCAAAACACCGTCTACCTCCTCCAGAGCCCCTCCACATCAACACTGGCGCCGCCGTCGTCTGGAGGTTGGTTGGTCAAGGTCGAAGAGTGCGACAGAATGCTTCTTCGGAATCCCATCACCTCCCGCCGAATCAGGTATTCAAACGACTCGTCGTCGTCTCCGGAATGTTTCAATTTGTTGGAGTTTAAAATGATCGAATTAGGGAAATCCTATTTTCTGAGATACATCCGTGGCTCTGGTTCTGTTGATGTGTTAAAGAAAGTGATTTTAATGCCTCCTGAGATTGTTGATTGTGCAATTCTGGTTATCTGTGATAAAGGAAATTTGGGTTTTGCTAAGAGTGGAGATGAGAAACTCACAAAATTCAGTAATGGAGGTTTTGTTTTTGATGATGTATTTGTTTATAAGGGTCAGCCATATGTGGTGGATAGCTTGGGTTGTGTTTTTAGGATTGATGTGTCCTCAAAAGTGATGGTGAAAATTGCGTCGGAATTGGCGGTTGGTTTAGGTGGGAGGAAACATTTTGTGGAGTCTTGCGGAGAGCTTTATGTGGTCGATAGGTATTTTGATCAAGAACAAAATAAGCAAGAAGCGACTGGTCTTGAGTTAGATTTTTCTTTTTGGGGCTTTCGTCGTCGTGGCAGATACCGGAGATATCATTCTGGTGAACTCAAGGTAGTTGATTTTAAGGTTTACAAGCTAGAACCGTTGGATGGAGAGTTGGGCAGATGGGTTGAAGTGGAAAGCTTGGGTGGTCAAGCGTTTTTCTTGGCTATAGATTGCTCTTTCTCTGTATTGGCTGAACAATTAGCTGGGTGCAAAGGGAATTGCATTTACTTCTCAGATGAAAATGATGTTAATCTTGCTCTTAGAGAAGTAATTAGACCAGAGACTTTTGTGTTCGACTTAGAGGACCATAGCATTCAGAAGTTGGAAACTTCCCCAGGCGGTTCCCAGATATTTTGGCCACCTCCAATTGAGCTCGGCTCCACGTGTTCGTATTCTCTGGATTGA

Protein Analysis

404

Amino Acids

45.37

Weight (kDa)

5.6

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 66 - 352 7.1e-41 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44080 AT1G57906 AT1G65735 AT1G65740 AT1G65760 AT1G65770 AT2G17690 AT2G26160 AT3G25750 AT3G25750 AT4G35733
fragaria_vesca FvH4_1g10810 FvH4_1g10830 FvH4_1g10840 FvH4_1g10840 FvH4_1g10840 FvH4_1g10860 FvH4_1g10880
malus_domestica MD02G1122000.v1.1 MD15G1235700.v1.1 MD15G1235900.v1.1
prunus_persica Prupe.7G177000_v2.0.a1 Prupe.7G177100_v2.0.a1 Prupe.7G177200_v2.0.a1 Prupe.7G177300_v2.0.a1 Prupe.7G177400_v2.0.a1 Prupe.7G177400_v2.0.a1
pyrus_communis pycom02g09500 pycom15g20990 pycom15g21000
rosa_chinensis RchiOBHm_Chr1g0325601 RchiOBHm_Chr2g0098071 RchiOBHm_Chr2g0098081 RchiOBHm_Chr2g0098091 RchiOBHm_Chr2g0098121 RchiOBHm_Chr2g0098131 RchiOBHm_Chr2g0098151 RchiOBHm_Chr2g0134711 RchiOBHm_Chr3g0456141 RchiOBHm_Chr4g0394811 RchiOBHm_Chr4g0416561 RchiOBHm_Chr5g0014321
rosa_laevigata RLG00000001178 RLG00000016753 RLG00000016754 RLG00000016755 RLG00000016756 RLG00000016757 RLG00000016758 RLG00000016760 RLG00000016761 RLG00000016762 RLG00000016763
rosa_multiflora Rmu_co8307469.1_g000001 Rmu_sc0000427.1_g000002 Rmu_sc0002880.1_g000004 Rmu_sc0002880.1_g000007 Rmu_sc0009178.1_g000019 Rmu_sc0013919.1_g000002 Rmu_sc0013919.1_g000003 Rmu_sc0014278.1_g000001 Rmu_sc0031755.1_g000001 Rmu_sc0031755.1_g000002 Rmu_sc0039043.1_g000001 Rmu_sc0039432.1_g000001
rosa_roxburghii Rroxscaffold_2G00144260 Rroxscaffold_2G00144270 Rroxscaffold_2G00144290 Rroxscaffold_2G00144310 Rroxscaffold_2G00144320 Rroxscaffold_2G00144340 Rroxscaffold_3G00238070
rosa_rugosa Rorug02G0069800 Rorug02G0069900 Rorug02G0070000 Rorug02G0070100 Rorug02G0070200 Rorug02G0070500 Rorug02G0070500 Rorug05G0053500 Rorug05G0482400 Rorug07G0244200
rosa_samantha Rh1DG210300 Rh2AG117100 Rh2AG117200 Rh2AG117300 Rh2AG117500 Rh2AG117700 Rh2AG117800 Rh2AG563300 Rh2BG119900 Rh2BG120000 Rh2BG120200 Rh2BG120300 Rh2BG120400 Rh2BG120600 Rh2BG120700 Rh2BG377200 Rh2CG076100 Rh2CG076200 Rh2CG121600 Rh2CG121700 Rh2CG121900 Rh2CG122000 Rh2CG122100 Rh2CG122200 Rh2CG122300 Rh2DG121600 Rh2DG121700 Rh2DG122000 Rh2DG122100 Rh2DG122300 Rh3AG138900 Rh3BG074100 Rh3CG073500 Rh3DG298600 Rh4CG222800 Rh4CG222900 Rh5CG120400 Rh6BG018400 Rh6BG124600 Rh6CG123200 Rh6CG123300 Rh7AG342600 Rh7AG342700 Rh7BG352100 Rh7CG128700 Rh7CG360200 Rh7CG360300
rosa_wichuraiana Rw2G009150 Rw2G009160 Rw2G009170 Rw2G009200 Rw4G016710 Rw5G035380 Rw6G043260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 244
AccI GTMKAC 2 cut(s) 15, 210
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 7 cut(s) 73, 94, 248, 322, 695, 735, 1146
AcoI YGGCCR 1 cut(s) 1163
AcsI RAATTY 3 cut(s) 155, 520, 560
AcvI CACGTG 1 cut(s) 1191
AcyI GRCGYC 1 cut(s) 245
AfiI CCNNNNNNNGG 4 cut(s) 18, 53, 257, 911
AflIII ACRYGT 1 cut(s) 1190
AgsI TTSAA 3 cut(s) 339, 370, 935
AhdI GACNNNNNGTC 1 cut(s) 349
AjnI CCWGG 1 cut(s) 1141
AjuI GAANNNNNNNTTGG 2 cut(s) 681, 713
AluBI AGCT 8 cut(s) 64, 70, 630, 742, 898, 945, 1010, 1180
AluI AGCT 8 cut(s) 64, 70, 630, 742, 898, 945, 1010, 1180
Alw21I GWGCWC 1 cut(s) 1182
Alw26I GTCTC 2 cut(s) 360, 1082
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 1 cut(s) 1163
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 3 cut(s) 155, 520, 560
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 3 cut(s) 21, 179, 1111
AsuHPI GGTGA 3 cut(s) 307, 685, 875
AvaII GGWCC 3 cut(s) 21, 179, 1111
BaeI ACNNNNGTAYC 2 cut(s) 412, 445
BalI TGGCCA 1 cut(s) 1165
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 2 cut(s) 227, 1182
BbrPI CACGTG 1 cut(s) 1191
Bbv12I GWGCWC 1 cut(s) 1182
BbvI GCAGC 1 cut(s) 108
BccI CCATC 5 cut(s) 175, 320, 664, 905, 921
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 1143
BclI TGATCA 1 cut(s) 766
BcoDI GTCTC 2 cut(s) 360, 1082
BfaI CTAG 1 cut(s) 899
BfmI CTRYAG 1 cut(s) 972
BfoI RGCGCY 1 cut(s) 248
BisI GCNGC 2 cut(s) 97, 248
BlsI GCNGC 2 cut(s) 98, 249
Bme1390I CCNGG 1 cut(s) 1143
Bme18I GGWCC 3 cut(s) 21, 179, 1111
BmeRI GACNNNNNGTC 1 cut(s) 349
BmgT120I GGNCC 3 cut(s) 21, 179, 1111
BmiI GGNNCC 4 cut(s) 181, 246, 1150, 1186
BmrFI CCNGG 1 cut(s) 1143
BmsI GCATC 1 cut(s) 84
BplI GAGNNNNNCTC 4 cut(s) 48, 80, 1169, 1201
BpmI CTGGAG 2 cut(s) 203, 279
BpuEI CTTGAG 3 cut(s) 50, 821, 854
BsaAI YACGTR 1 cut(s) 1191
BsaBI GATNNNNATC 1 cut(s) 1056
BsaHI GRCGYC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 427, 1141
BsaWI WCCGGW 2 cut(s) 358, 846
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 53, 257, 911
Bse1I ACTGG 4 cut(s) 23, 32, 246, 799
Bse8I GATNNNNATC 1 cut(s) 1056
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 1 cut(s) 1143
BseDI CCNNGG 2 cut(s) 427, 1141
BseGI GGATG 4 cut(s) 10, 167, 423, 916
BseJI GATNNNNATC 1 cut(s) 1056
BseLI CCNNNNNNNGG 4 cut(s) 18, 53, 257, 911
BseMII CTCAG 3 cut(s) 407, 468, 1053
BseNI ACTGG 4 cut(s) 23, 32, 246, 799
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 1010
BshFI GGCC 1 cut(s) 1165
BshNI GGYRCC 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 1182
BsiSI CCGG 2 cut(s) 359, 847
BslI CCNNNNNNNGG 4 cut(s) 18, 53, 257, 911
BsmAI GTCTC 2 cut(s) 360, 1082
BsmBI CGTCTC 1 cut(s) 360
BsmI GAATGC 2 cut(s) 300, 1119
BsnI GGCC 1 cut(s) 1165
Bsp1286I GDGCHC 2 cut(s) 227, 1182
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 3 cut(s) 51, 390, 766
BspACI CCGC 7 cut(s) 73, 94, 248, 322, 695, 735, 1146
BspANI GGCC 1 cut(s) 1165
BspCNI CTCAG 3 cut(s) 408, 469, 1052
BspEI TCCGGA 1 cut(s) 358
BspLI GGNNCC 4 cut(s) 181, 246, 1150, 1186
BspT107I GGYRCC 1 cut(s) 244
BsrI ACTGG 4 cut(s) 23, 32, 246, 799
BssECI CCNNGG 2 cut(s) 427, 1141
BssMI GATC 3 cut(s) 51, 390, 766
BssNI GRCGYC 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 1143
Bst4CI ACNGT 3 cut(s) 13, 208, 906
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 245
BstAPI GCANNNNNTGC 1 cut(s) 102
BstBAI YACGTR 1 cut(s) 1191
BstDEI CTNAG 6 cut(s) 416, 477, 534, 1039, 1067, 1105
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 4 cut(s) 10, 167, 423, 916
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 3 cut(s) 54, 393, 769
BstMAI GTCTC 2 cut(s) 360, 1082
BstMBI GATC 3 cut(s) 51, 390, 766
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstNI CCWGG 1 cut(s) 1143
BstSCI CCNGG 1 cut(s) 1141
BstSFI CTRYAG 1 cut(s) 972
BstV1I GCAGC 1 cut(s) 108
BsuRI GGCC 1 cut(s) 1165
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 4 cut(s) 10, 167, 423, 916
BtsIMutI CAGTG 2 cut(s) 47, 239
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 3 cut(s) 21, 179, 1111
CseI GACGC 1 cut(s) 672
CviAII CATG 1 cut(s) 111
DdeI CTNAG 6 cut(s) 416, 477, 534, 1039, 1067, 1105
DinI GGCGCC 1 cut(s) 246
DpnI GATC 3 cut(s) 53, 392, 768
DpnII GATC 3 cut(s) 51, 390, 766
DraI TTTAAA 1 cut(s) 385
DriI GACNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 1163
Eam1104I CTCTTC 1 cut(s) 276
Eam1105I GACNNNNNGTC 1 cut(s) 349
EarI CTCTTC 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 1180
Eco24I GRGCYC 2 cut(s) 227, 1182
Eco32I GATATC 1 cut(s) 854
Eco47I GGWCC 3 cut(s) 21, 179, 1111
Eco53kI GAGCTC 1 cut(s) 1180
Eco72I CACGTG 1 cut(s) 1191
EcoICRI GAGCTC 1 cut(s) 1180
EcoRII CCWGG 1 cut(s) 1141
EcoRV GATATC 1 cut(s) 854
EcoT38I GRGCYC 2 cut(s) 227, 1182
EgeI GGCGCC 1 cut(s) 246
EheI GGCGCC 1 cut(s) 246
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 1 cut(s) 114
FaiI YATR 7 cut(s) 112, 603, 616, 618, 747, 974, 1116
FatI CATG 1 cut(s) 110
FauI CCCGC 1 cut(s) 329
FauNDI CATATG 1 cut(s) 616
FbaI TGATCA 1 cut(s) 766
FblI GTMKAC 2 cut(s) 15, 210
Fnu4HI GCNGC 2 cut(s) 97, 248
FokI GGATG 4 cut(s) 17, 154, 410, 923
FriOI GRGCYC 2 cut(s) 227, 1182
Fsp4HI GCNGC 2 cut(s) 97, 248
FspBI CTAG 1 cut(s) 899
GlaI GCGC 1 cut(s) 246
GluI GCNGC 2 cut(s) 97, 248
GsaI CCCAGC 1 cut(s) 1014
GsuI CTGGAG 2 cut(s) 203, 279
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 1 cut(s) 1165
HapII CCGG 2 cut(s) 359, 847
HgaI GACGC 1 cut(s) 672
HhaI GCGC 1 cut(s) 247
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 1 cut(s) 114
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 6 cut(s) 80, 90, 307, 327, 344, 728
HpaII CCGG 2 cut(s) 359, 847
HphI GGTGA 3 cut(s) 307, 685, 875
Hpy166II GTNNAC 4 cut(s) 16, 211, 866, 892
Hpy188I TCNGA 7 cut(s) 56, 89, 306, 417, 688, 1042, 1125
Hpy188III TCNNGA 7 cut(s) 220, 258, 359, 476, 770, 800, 1205
Hpy8I GTNNAC 4 cut(s) 16, 211, 866, 892
Hpy99I CGWCG 7 cut(s) 17, 256, 352, 355, 688, 834, 837
HpyAV CCTTC 2 cut(s) 150, 199
HpyCH4III ACNGT 3 cut(s) 13, 208, 906
HpyCH4IV ACGT 1 cut(s) 1190
HpyCH4V TGCA 3 cut(s) 494, 1017, 1029
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 6 cut(s) 416, 477, 534, 1039, 1067, 1105
HpySE526I ACGT 1 cut(s) 1190
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 1 cut(s) 114
HspAI GCGC 1 cut(s) 245
KasI GGCGCC 1 cut(s) 244
Kpn2I TCCGGA 1 cut(s) 358
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 3 cut(s) 51, 390, 766
LmnI GCTCC 2 cut(s) 75, 1190
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 84
MaeI CTAG 1 cut(s) 899
MaeII ACGT 1 cut(s) 1190
MalI GATC 3 cut(s) 53, 392, 768
MboI GATC 3 cut(s) 51, 390, 766
MboII GAAGA 3 cut(s) 20, 293, 293
MfeI CAATTG 1 cut(s) 1173
MhlI GDGCHC 2 cut(s) 227, 1182
MlsI TGGCCA 1 cut(s) 1165
MluNI TGGCCA 1 cut(s) 1165
Mly113I GGCGCC 1 cut(s) 245
MlyI GAGTC 3 cut(s) 74, 338, 737
MmeI TCCRAC 4 cut(s) 357, 666, 888, 1110
Mox20I TGGCCA 1 cut(s) 1165
MroI TCCGGA 1 cut(s) 358
MscI TGGCCA 1 cut(s) 1165
MseI TTAA 5 cut(s) 384, 452, 467, 885, 1056
MslI CAYNNNNRTG 1 cut(s) 861
Msp20I TGGCCA 1 cut(s) 1165
MspI CCGG 2 cut(s) 359, 847
MspR9I CCNGG 1 cut(s) 1143
MunI CAATTG 1 cut(s) 1173
Mva1269I GAATGC 2 cut(s) 300, 1119
MvaI CCWGG 1 cut(s) 1143
MwoI GCNNNNNNNGC 1 cut(s) 102
NarI GGCGCC 1 cut(s) 245
NdeI CATATG 1 cut(s) 616
NdeII GATC 3 cut(s) 51, 390, 766
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 4 cut(s) 181, 246, 1150, 1186
NmeAIII GCCGAG 1 cut(s) 1161
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 2 cut(s) 300, 1119
PfeI GAWTC 3 cut(s) 90, 307, 327
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 98, 249
PleI GAGTC 3 cut(s) 74, 338, 736
PluTI GGCGCC 1 cut(s) 248
PmaCI CACGTG 1 cut(s) 1191
PmlI CACGTG 1 cut(s) 1191
PpsI GAGTC 3 cut(s) 74, 338, 736
Ppu21I YACGTR 1 cut(s) 1191
PsiI TTATAA 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 1182
Psp6I CCWGG 1 cut(s) 1141
PspCI CACGTG 1 cut(s) 1191
PspFI CCCAGC 1 cut(s) 1010
PspGI CCWGG 1 cut(s) 1141
PspN4I GGNNCC 4 cut(s) 181, 246, 1150, 1186
PspPI GGNCC 3 cut(s) 21, 179, 1111
PsyI GACNNNGTC 1 cut(s) 11
RseI CAYNNNNRTG 1 cut(s) 861
SacI GAGCTC 1 cut(s) 1182
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 5 cut(s) 384, 452, 467, 885, 1056
SatI GCNGC 2 cut(s) 97, 248
Sau3AI GATC 3 cut(s) 51, 390, 766
Sau96I GGNCC 3 cut(s) 21, 179, 1111
SchI GAGTC 3 cut(s) 74, 338, 737
ScrFI CCNGG 1 cut(s) 1143
SduI GDGCHC 2 cut(s) 227, 1182
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 1 cut(s) 972
SfoI GGCGCC 1 cut(s) 246
SinI GGWCC 3 cut(s) 21, 179, 1111
SmiMI CAYNNNNRTG 1 cut(s) 861
SmlI CTYRAG 3 cut(s) 65, 800, 869
SmoI CTYRAG 3 cut(s) 65, 800, 869
SsiI CCGC 7 cut(s) 73, 94, 248, 322, 695, 735, 1146
SspDI GGCGCC 1 cut(s) 244
SspMI CTAG 1 cut(s) 899
SstI GAGCTC 1 cut(s) 1182
StyD4I CCNGG 1 cut(s) 1141
TaaI ACNGT 3 cut(s) 13, 208, 906
TaiI ACGT 1 cut(s) 1193
TaqI TCGA 7 cut(s) 15, 36, 78, 279, 393, 753, 1101
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 3 cut(s) 90, 307, 327
Tru1I TTAA 5 cut(s) 384, 452, 467, 885, 1056
Tru9I TTAA 5 cut(s) 384, 452, 467, 885, 1056
TscAI CASTG 2 cut(s) 54, 246
TseI GCWGC 1 cut(s) 96
TspDTI ATGAA 2 cut(s) 99, 1059
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 2 cut(s) 54, 246
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 3 cut(s) 21, 179, 1111
XapI RAATTY 3 cut(s) 155, 520, 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmiI GTMKAC 2 cut(s) 15, 210
XspI CTAG 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.