Rw4G016710

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000246
Physical Location & Seq
Forward (+)
37974500 .. 37975805
1306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016710.1

Sequence Viewer

Length: 999 bp
ATGGATATCACAGTCGACTTGTCCGATCTTCCAGTCGAGCTCTGGCCACTGATCGGAGAAAAGCTCGAACTGCGCATCCAGTCTCTCCAATTCCGCAGCGTTTGTCATTCATGGCATTCTTGTGCCTCACTTCCACCCTTCACTTCCTCCGAAATTCCCTCTCCCATTCTCCGATGGACCCACCTCCTTCCTCTCCCAAAACACCGTCTACCTCCTACAGAGCCCCTTCACGTCATCACTGGCGCCACCGTCGTTTGGAGGTTGGTTGGGAAGTCGTATTTTCTTAGATACATCCTTAGCTCTAGTTCTGTTTGTGGTTTAAAGAAAGTAATTTTAATGCCTGCTGAGATTGTTGATTGTGCTATTCTGGTTATCTCTGATAAGGGGAATTTGGGTTTTGCTAAAAGTGGAGATGAGAAACTCACATGGTTTAGTAATGGAGGTTTTGTTTTTGATGATGTTATTGTCTATAAGGGTCAGCCATATGTGGTGGATAGCTTGAGTTATGTTTTTAAGATTGATGTGTCTTCAGAAGTGATGGTGAAGATTTCATCAAAAGTGATTGGTTTTGGTGGGAGGAAGCATTTGGTAGAGTCTTGTGGAGAGCTTTATGTGGTCGATATGTATTTTGATCAAGAACAAAATAAGCAAGAAGGGGCTGGTGTTGAGTTAGATTTTTCTTTATTTTTGGAACTGTTGAATGGAGAGTTGGGCAGATGGGTTGAAGTGAAGTGCTTGGGTGATCGAGCGTTTTTCTTGGCTATGGATTGTTGTTTCTCTGTTTGGCACAAGAATTGGCTAGATGAAAACAATGTTAATCTTGCTCTTAGAGAAGTAACTAGACCAGAGAGTTTCATGTTCTACTTAGAGGACCATAGCATTCAGAAGTTGGGATCTTCCCCAAGCGGTTCCCAGATATTTTGGCCACCTCCAATTGAGCTCGGCTCCTTGCGTTCCTTTTCCATGTTTAACTTCATGTTAGATGATTTATTTGTATAG

Protein Analysis

332

Amino Acids

37.57

Weight (kDa)

5.23

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 44 - 265 1.4e-23 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44080 AT1G57906 AT1G65735 AT1G65740 AT1G65760 AT1G65770 AT2G17690 AT2G26160 AT3G25750 AT3G25750 AT4G35733
fragaria_vesca FvH4_1g10810 FvH4_1g10830 FvH4_1g10840 FvH4_1g10840 FvH4_1g10840 FvH4_1g10860 FvH4_1g10880
malus_domestica MD02G1122000.v1.1 MD15G1235700.v1.1 MD15G1235900.v1.1
prunus_persica Prupe.7G177000_v2.0.a1 Prupe.7G177100_v2.0.a1 Prupe.7G177200_v2.0.a1 Prupe.7G177300_v2.0.a1 Prupe.7G177400_v2.0.a1 Prupe.7G177400_v2.0.a1
pyrus_communis pycom02g09500 pycom15g20990 pycom15g21000
rosa_chinensis RchiOBHm_Chr1g0325601 RchiOBHm_Chr2g0098071 RchiOBHm_Chr2g0098081 RchiOBHm_Chr2g0098091 RchiOBHm_Chr2g0098121 RchiOBHm_Chr2g0098131 RchiOBHm_Chr2g0098151 RchiOBHm_Chr2g0134711 RchiOBHm_Chr3g0456141 RchiOBHm_Chr4g0394811 RchiOBHm_Chr4g0416561 RchiOBHm_Chr5g0014321
rosa_laevigata RLG00000001178 RLG00000016753 RLG00000016754 RLG00000016755 RLG00000016756 RLG00000016757 RLG00000016758 RLG00000016760 RLG00000016761 RLG00000016762 RLG00000016763
rosa_multiflora Rmu_co8307469.1_g000001 Rmu_sc0000427.1_g000002 Rmu_sc0002880.1_g000004 Rmu_sc0002880.1_g000007 Rmu_sc0009178.1_g000019 Rmu_sc0013919.1_g000002 Rmu_sc0013919.1_g000003 Rmu_sc0014278.1_g000001 Rmu_sc0031755.1_g000001 Rmu_sc0031755.1_g000002 Rmu_sc0039043.1_g000001 Rmu_sc0039432.1_g000001
rosa_roxburghii Rroxscaffold_2G00144260 Rroxscaffold_2G00144270 Rroxscaffold_2G00144290 Rroxscaffold_2G00144310 Rroxscaffold_2G00144320 Rroxscaffold_2G00144340 Rroxscaffold_3G00238070
rosa_rugosa Rorug02G0069800 Rorug02G0069900 Rorug02G0070000 Rorug02G0070100 Rorug02G0070200 Rorug02G0070500 Rorug02G0070500 Rorug05G0053500 Rorug05G0482400 Rorug07G0244200
rosa_samantha Rh1DG210300 Rh2AG117100 Rh2AG117200 Rh2AG117300 Rh2AG117500 Rh2AG117700 Rh2AG117800 Rh2AG563300 Rh2BG119900 Rh2BG120000 Rh2BG120200 Rh2BG120300 Rh2BG120400 Rh2BG120600 Rh2BG120700 Rh2BG377200 Rh2CG076100 Rh2CG076200 Rh2CG121600 Rh2CG121700 Rh2CG121900 Rh2CG122000 Rh2CG122100 Rh2CG122200 Rh2CG122300 Rh2DG121600 Rh2DG121700 Rh2DG122000 Rh2DG122100 Rh2DG122300 Rh3AG138900 Rh3BG074100 Rh3CG073500 Rh3DG298600 Rh4CG222800 Rh4CG222900 Rh5CG120400 Rh6BG018400 Rh6BG124600 Rh6CG123200 Rh6CG123300 Rh7AG342600 Rh7AG342700 Rh7BG352100 Rh7CG128700 Rh7CG360200 Rh7CG360300
rosa_wichuraiana Rw2G009150 Rw2G009160 Rw2G009170 Rw2G009200 Rw4G016710 Rw5G035380 Rw6G043260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 242
AccI GTMKAC 2 cut(s) 15, 208
AciI CCGC 2 cut(s) 94, 906
AclWI GGATC 1 cut(s) 901
AcoI YGGCCR 2 cut(s) 44, 923
AcsI RAATTY 2 cut(s) 153, 388
AcuI CTGAAG 1 cut(s) 513
AcyI GRCGYC 1 cut(s) 243
AfiI CCNNNNNNNGG 2 cut(s) 53, 255
AgsI TTSAA 2 cut(s) 700, 725
AjiI CACGTC 1 cut(s) 232
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AloI GAACNNNNNNTCC 2 cut(s) 60, 92
AluBI AGCT 6 cut(s) 40, 64, 300, 498, 607, 940
AluI AGCT 6 cut(s) 40, 64, 300, 498, 607, 940
Alw21I GWGCWC 2 cut(s) 42, 942
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 1 cut(s) 901
AoxI GGCC 2 cut(s) 44, 923
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 2 cut(s) 153, 388
AspLEI GCGC 2 cut(s) 75, 245
AspS9I GGNCC 2 cut(s) 177, 871
AsuHPI GGTGA 2 cut(s) 553, 752
AvaII GGWCC 2 cut(s) 177, 871
BalI TGGCCA 2 cut(s) 46, 925
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 3 cut(s) 42, 225, 942
BbsI GAAGAC 1 cut(s) 519
Bbv12I GWGCWC 2 cut(s) 42, 942
BbvI GCAGC 1 cut(s) 108
BccI CCATC 3 cut(s) 168, 532, 711
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 1 cut(s) 87
BfaI CTAG 3 cut(s) 303, 800, 840
BfmI CTRYAG 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 246
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme18I GGWCC 2 cut(s) 177, 871
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 2 cut(s) 177, 871
BmiI GGNNCC 4 cut(s) 179, 244, 910, 946
BmsI GCATC 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 519
BplI GAGNNNNNCTC 4 cut(s) 48, 80, 929, 961
Bpu10I CCTNAGC 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 520
BsaHI GRCGYC 1 cut(s) 243
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 255
Bse1I ACTGG 3 cut(s) 32, 79, 244
BseGI GGATG 2 cut(s) 75, 291
BseLI CCNNNNNNNGG 2 cut(s) 53, 255
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 3 cut(s) 32, 79, 244
BseXI GCAGC 1 cut(s) 108
BshFI GGCC 2 cut(s) 46, 925
BshNI GGYRCC 1 cut(s) 242
BsiHKAI GWGCWC 2 cut(s) 42, 942
BslI CCNNNNNNNGG 2 cut(s) 53, 255
BsmAI GTCTC 1 cut(s) 87
BsmI GAATGC 2 cut(s) 115, 879
BsnI GGCC 2 cut(s) 46, 925
Bsp1286I GDGCHC 3 cut(s) 42, 225, 942
Bsp143I GATC 5 cut(s) 25, 51, 631, 742, 893
BspACI CCGC 2 cut(s) 94, 906
BspANI GGCC 2 cut(s) 46, 925
BspCNI CTCAG 1 cut(s) 337
BspLI GGNNCC 4 cut(s) 179, 244, 910, 946
BspPI GGATC 1 cut(s) 901
BspT107I GGYRCC 1 cut(s) 242
BsrI ACTGG 3 cut(s) 32, 79, 244
BssMI GATC 5 cut(s) 25, 51, 631, 742, 893
BssNI GRCGYC 1 cut(s) 243
Bst4CI ACNGT 4 cut(s) 13, 206, 250, 696
BstACI GRCGYC 1 cut(s) 243
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 5 cut(s) 284, 296, 345, 827, 865
BstF5I GGATG 2 cut(s) 75, 291
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 2 cut(s) 75, 245
BstKTI GATC 5 cut(s) 28, 54, 634, 745, 896
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 5 cut(s) 25, 51, 631, 742, 893
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 519
BstX2I RGATCY 1 cut(s) 893
BstYI RGATCY 1 cut(s) 893
BsuRI GGCC 2 cut(s) 46, 925
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 2 cut(s) 75, 291
BtsIMutI CAGTG 2 cut(s) 47, 237
Cac8I GCNNGC 1 cut(s) 342
CfoI GCGC 2 cut(s) 75, 245
Cfr13I GGNCC 2 cut(s) 177, 871
CviAII CATG 5 cut(s) 111, 426, 856, 964, 976
DdeI CTNAG 5 cut(s) 284, 296, 345, 827, 865
DinI GGCGCC 1 cut(s) 244
DpnI GATC 5 cut(s) 27, 53, 633, 744, 895
DpnII GATC 5 cut(s) 25, 51, 631, 742, 893
DraI TTTAAA 1 cut(s) 321
EaeI YGGCCR 2 cut(s) 44, 923
Ecl136II GAGCTC 2 cut(s) 40, 940
Eco24I GRGCYC 3 cut(s) 42, 225, 942
Eco32I GATATC 1 cut(s) 7
Eco47I GGWCC 2 cut(s) 177, 871
Eco53kI GAGCTC 2 cut(s) 40, 940
Eco57I CTGAAG 1 cut(s) 513
EcoICRI GAGCTC 2 cut(s) 40, 940
EcoRV GATATC 1 cut(s) 7
EcoT38I GRGCYC 3 cut(s) 42, 225, 942
EgeI GGCGCC 1 cut(s) 244
EheI GGCGCC 1 cut(s) 244
FaeI CATG 5 cut(s) 114, 429, 859, 967, 979
FatI CATG 5 cut(s) 110, 425, 855, 963, 975
FauNDI CATATG 1 cut(s) 484
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 2 cut(s) 15, 208
Fnu4HI GCNGC 1 cut(s) 97
FokI GGATG 2 cut(s) 62, 278
FriOI GRGCYC 3 cut(s) 42, 225, 942
Fsp4HI GCNGC 1 cut(s) 97
FspBI CTAG 3 cut(s) 303, 800, 840
FspI TGCGCA 1 cut(s) 74
GlaI GCGC 2 cut(s) 74, 244
GluI GCNGC 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 2 cut(s) 46, 925
HhaI GCGC 2 cut(s) 75, 245
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 5 cut(s) 114, 429, 859, 967, 979
Hin6I GCGC 2 cut(s) 73, 243
HinP1I GCGC 2 cut(s) 73, 243
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 1 cut(s) 593
HphI GGTGA 2 cut(s) 553, 752
Hpy166II GTNNAC 2 cut(s) 16, 209
Hpy188I TCNGA 7 cut(s) 25, 56, 151, 173, 379, 532, 885
Hpy188III TCNNGA 1 cut(s) 635
Hpy8I GTNNAC 2 cut(s) 16, 209
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 4 cut(s) 148, 197, 236, 647
HpyCH4III ACNGT 4 cut(s) 13, 206, 250, 696
HpyCH4IV ACGT 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 5 cut(s) 284, 296, 345, 827, 865
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 5 cut(s) 114, 429, 859, 967, 979
HspAI GCGC 2 cut(s) 73, 243
KasI GGCGCC 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 5 cut(s) 25, 51, 631, 742, 893
LmnI GCTCC 1 cut(s) 950
LpnPI CCDG 9 cut(s) 28, 45, 92, 225, 353, 354, 645, 858, 926
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 84
MaeI CTAG 3 cut(s) 303, 800, 840
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 835
MalI GATC 5 cut(s) 27, 53, 633, 744, 895
MboI GATC 5 cut(s) 25, 51, 631, 742, 893
MboII GAAGA 4 cut(s) 20, 519, 556, 888
MfeI CAATTG 1 cut(s) 933
MflI RGATCY 1 cut(s) 893
MhlI GDGCHC 3 cut(s) 42, 225, 942
MlsI TGGCCA 2 cut(s) 46, 925
MluCI AATT 6 cut(s) 89, 153, 330, 388, 793, 933
MluNI TGGCCA 2 cut(s) 46, 925
Mly113I GGCGCC 1 cut(s) 243
MlyI GAGTC 1 cut(s) 602
Mox20I TGGCCA 2 cut(s) 46, 925
MscI TGGCCA 2 cut(s) 46, 925
MseI TTAA 5 cut(s) 320, 335, 513, 816, 969
MslI CAYNNNNRTG 1 cut(s) 120
Msp20I TGGCCA 2 cut(s) 46, 925
MunI CAATTG 1 cut(s) 933
Mva1269I GAATGC 2 cut(s) 115, 879
MwoI GCNNNNNNNGC 1 cut(s) 70
NarI GGCGCC 1 cut(s) 243
NdeI CATATG 1 cut(s) 484
NdeII GATC 5 cut(s) 25, 51, 631, 742, 893
NlaIII CATG 5 cut(s) 114, 429, 859, 967, 979
NlaIV GGNNCC 4 cut(s) 179, 244, 910, 946
NmeAIII GCCGAG 1 cut(s) 921
NsbI TGCGCA 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 2 cut(s) 115, 879
PkrI GCNGC 1 cut(s) 98
PleI GAGTC 1 cut(s) 601
PluTI GGCGCC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 601
Psp124BI GAGCTC 2 cut(s) 42, 942
PspN4I GGNNCC 4 cut(s) 179, 244, 910, 946
PspPI GGNCC 2 cut(s) 177, 871
PsuI RGATCY 1 cut(s) 893
RseI CAYNNNNRTG 1 cut(s) 120
SacI GAGCTC 2 cut(s) 42, 942
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 5 cut(s) 320, 335, 513, 816, 969
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 5 cut(s) 25, 51, 631, 742, 893
Sau96I GGNCC 2 cut(s) 177, 871
SchI GAGTC 1 cut(s) 602
SduI GDGCHC 3 cut(s) 42, 225, 942
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 1 cut(s) 216
SfoI GGCGCC 1 cut(s) 244
SinI GGWCC 2 cut(s) 177, 871
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
Sse9I AATT 6 cut(s) 89, 153, 330, 388, 793, 933
SsiI CCGC 2 cut(s) 94, 906
SspDI GGCGCC 1 cut(s) 242
SspMI CTAG 3 cut(s) 303, 800, 840
SstI GAGCTC 2 cut(s) 42, 942
TaaI ACNGT 4 cut(s) 13, 206, 250, 696
TaiI ACGT 1 cut(s) 234
TaqI TCGA 5 cut(s) 15, 36, 66, 618, 745
TasI AATT 6 cut(s) 89, 153, 330, 388, 793, 933
Tru1I TTAA 5 cut(s) 320, 335, 513, 816, 969
Tru9I TTAA 5 cut(s) 320, 335, 513, 816, 969
TscAI CASTG 2 cut(s) 54, 244
TseI GCWGC 1 cut(s) 96
TspDTI ATGAA 5 cut(s) 99, 540, 819, 844, 964
TspRI CASTG 2 cut(s) 54, 244
VpaK11BI GGWCC 2 cut(s) 177, 871
XapI RAATTY 2 cut(s) 153, 388
XcmI CCANNNNNNNNNTGG 1 cut(s) 39
XmiI GTMKAC 2 cut(s) 15, 208
XspI CTAG 3 cut(s) 303, 800, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.