Rh6AG091200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000448
Physical Location & Seq
Forward (+)
13537713 .. 13540414
2702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG091200.1

Sequence Viewer

Length: 804 bp
ATGGCAAATGCATCTGAGGTAGCTCCGCCTGCGGCCATTTTCATATTTGGAGACTCAACTGTGGATGTTGGCACCAATACCTTGTTGCTCCAAAGTGGAGCAAGGGCTGATTTCTCTCCAAATGGGGTTGATTTTCCTTATTCTGTGCCTACTGGGAGGTTCAGCAATGGCCTTAACAGCGCTGACCAAATCGTGAAACTATTTGGACGCCGGAAAAGTCCACCACCTTTCTTGTATGCCGCGAGTCATATGTCCACTTTTAAAAGGAATATACTGCAGGGAGTCAACTTTGCTTCAGCAGGAAGTGGCATCTTCGAAGACACGGGGATAAAACGATGGACAGAAGTTGTGTCACTGGGAAATCAGATCCAACAATTTGCAGCGGTGCGTGGAAATTTCACAGAGATAGTTGGTTTCAAAACAACTGATACCGTGCTTTCCAAGTCTTTGTTCATCATCAGTATCGGAAGCAACGATCTTTTCGAATTAGTTGAGTACTTTCCAAATGCCACGGACTTGTTTAAGGCGGAACACATGGAACGTCTTCAACTAACGTATAAGAACCATTTGAAGAATTTATACAAGCTGGGAGCTCGGAAATTCGGGATTATAAGTGTTCCTCCAATTGGATGTTGTCCATATGCGCGTGTTCAACCAAATGTCGACCCTAGTGTTTGTGTGAAGGAACTAAACAAGCTTGCTCAAACATTTTTCATAGAAACACACGCTCTCCTGCGGAAATTGAGCTCGGATTTGAAAGGGATGAGGACGTTCAATCAGCCTGCTGTGGTTACGGGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

267

Amino Acids

29.21

Weight (kDa)

9.24

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 13 - 253 4.7e-20 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 611
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 663
AccII CGCG 2 cut(s) 242, 646
AciI CCGC 6 cut(s) 26, 32, 240, 383, 527, 736
AclWI GGATC 1 cut(s) 361
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 3 cut(s) 394, 574, 599
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 208
AfaI GTAC 1 cut(s) 497
AfeI AGCGCT 1 cut(s) 181
AfiI CCNNNNNNNGG 1 cut(s) 626
AgsI TTSAA 6 cut(s) 418, 548, 571, 653, 757, 775
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 5 cut(s) 23, 586, 593, 697, 747
AluI AGCT 5 cut(s) 23, 586, 593, 697, 747
Alw21I GWGCWC 2 cut(s) 595, 749
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 1 cut(s) 361
Aor51HI AGCGCT 1 cut(s) 181
AoxI GGCC 2 cut(s) 33, 169
ApeKI GCWGC 1 cut(s) 380
ApoI RAATTY 3 cut(s) 394, 574, 599
Asp700I GAANNNNTTC 1 cut(s) 543
AspLEI GCGC 2 cut(s) 182, 646
AsuII TTCGAA 2 cut(s) 315, 483
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 595, 749
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BbsI GAAGAC 2 cut(s) 324, 536
Bbv12I GWGCWC 2 cut(s) 595, 749
BbvI GCAGC 1 cut(s) 392
BccI CCATC 1 cut(s) 330
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 1 cut(s) 669
BfmI CTRYAG 1 cut(s) 275
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 3 cut(s) 33, 240, 381
BlsI GCNGC 3 cut(s) 34, 241, 382
BmcAI AGTACT 1 cut(s) 497
BmiI GGNNCC 1 cut(s) 73
BmrI ACTGGG 2 cut(s) 162, 365
BmsI GCATC 2 cut(s) 20, 318
BmuI ACTGGG 2 cut(s) 162, 365
BpiI GAAGAC 2 cut(s) 324, 536
Bpu14I TTCGAA 2 cut(s) 315, 483
BsaHI GRCGYC 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 510
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 1 cut(s) 626
Bse1I ACTGG 2 cut(s) 157, 360
Bse3DI GCAATG 1 cut(s) 172
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 3 cut(s) 70, 635, 768
BseLI CCNNNNNNNGG 1 cut(s) 626
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 6
BseNI ACTGG 2 cut(s) 157, 360
BseXI GCAGC 1 cut(s) 392
BseYI CCCAGC 1 cut(s) 586
Bsh1236I CGCG 2 cut(s) 242, 646
BshFI GGCC 2 cut(s) 35, 171
BshNI GGYRCC 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 595, 749
BsiSI CCGG 1 cut(s) 211
BslI CCNNNNNNNGG 1 cut(s) 626
BsmAI GTCTC 1 cut(s) 45
BsnI GGCC 2 cut(s) 35, 171
Bsp119I TTCGAA 2 cut(s) 315, 483
Bsp1286I GDGCHC 2 cut(s) 595, 749
Bsp143I GATC 2 cut(s) 366, 475
BspACI CCGC 6 cut(s) 26, 32, 240, 383, 527, 736
BspANI GGCC 2 cut(s) 35, 171
BspCNI CTCAG 1 cut(s) 7
BspFNI CGCG 2 cut(s) 242, 646
BspLI GGNNCC 1 cut(s) 73
BspMAI CTGCAG 1 cut(s) 279
BspPI GGATC 1 cut(s) 361
BspT104I TTCGAA 2 cut(s) 315, 483
BspT107I GGYRCC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 172
BsrI ACTGG 2 cut(s) 157, 360
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 2 cut(s) 366, 475
BssNI GRCGYC 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 61, 433
BstACI GRCGYC 1 cut(s) 208
BstBI TTCGAA 2 cut(s) 315, 483
BstC8I GCNNGC 3 cut(s) 30, 699, 783
BstDEI CTNAG 1 cut(s) 15
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 3 cut(s) 70, 635, 768
BstFNI CGCG 2 cut(s) 242, 646
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 2 cut(s) 182, 646
BstKTI GATC 2 cut(s) 369, 478
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 2 cut(s) 366, 475
BstMWI GCNNNNNNNGC 2 cut(s) 29, 177
BstSFI CTRYAG 1 cut(s) 275
BstUI CGCG 2 cut(s) 242, 646
BstV1I GCAGC 1 cut(s) 392
BstV2I GAAGAC 2 cut(s) 324, 536
BstX2I RGATCY 1 cut(s) 366
BstYI RGATCY 1 cut(s) 366
BsuRI GGCC 2 cut(s) 35, 171
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 3 cut(s) 70, 635, 768
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 3 cut(s) 30, 699, 783
CfoI GCGC 2 cut(s) 182, 646
CseI GACGC 1 cut(s) 216
Csp6I GTAC 1 cut(s) 496
CspCI CAANNNNNGTGG 2 cut(s) 213, 248
CviAII CATG 1 cut(s) 535
CviJI RGCY 9 cut(s) 23, 35, 107, 171, 586, 593, 697, 747, 781
CviKI_1 RGCY 9 cut(s) 23, 35, 107, 171, 586, 593, 697, 747, 781
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 1 cut(s) 15
DpnI GATC 2 cut(s) 368, 477
DpnII GATC 2 cut(s) 366, 475
DraI TTTAAA 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 33
EciI GGCGGA 2 cut(s) 15, 542
Ecl136II GAGCTC 2 cut(s) 593, 747
Eco24I GRGCYC 2 cut(s) 595, 749
Eco47III AGCGCT 1 cut(s) 181
Eco53kI GAGCTC 2 cut(s) 593, 747
Eco57I CTGAAG 1 cut(s) 279
EcoICRI GAGCTC 2 cut(s) 593, 747
EcoT22I ATGCAT 1 cut(s) 13
EcoT38I GRGCYC 2 cut(s) 595, 749
FaeI CATG 1 cut(s) 538
FatI CATG 1 cut(s) 534
FauNDI CATATG 2 cut(s) 249, 640
FblI GTMKAC 1 cut(s) 663
Fnu4HI GCNGC 3 cut(s) 33, 240, 381
FokI GGATG 3 cut(s) 77, 642, 775
FriOI GRGCYC 2 cut(s) 595, 749
Fsp4HI GCNGC 3 cut(s) 33, 240, 381
FspBI CTAG 1 cut(s) 669
GlaI GCGC 2 cut(s) 181, 645
GluI GCNGC 3 cut(s) 33, 240, 381
GsaI CCCAGC 1 cut(s) 590
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 35, 171
HapII CCGG 1 cut(s) 211
HgaI GACGC 1 cut(s) 216
HhaI GCGC 2 cut(s) 182, 646
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 1 cut(s) 538
Hin6I GCGC 2 cut(s) 180, 644
HinP1I GCGC 2 cut(s) 180, 644
HincII GTYRAC 2 cut(s) 286, 664
HindII GTYRAC 2 cut(s) 286, 664
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 3 cut(s) 53, 244, 282
HpaII CCGG 1 cut(s) 211
Hpy166II GTNNAC 4 cut(s) 221, 255, 286, 664
Hpy188I TCNGA 5 cut(s) 16, 366, 467, 597, 751
Hpy188III TCNNGA 2 cut(s) 193, 604
Hpy8I GTNNAC 4 cut(s) 221, 255, 286, 664
HpyAV CCTTC 1 cut(s) 676
HpyCH4III ACNGT 2 cut(s) 61, 433
HpyCH4IV ACGT 3 cut(s) 541, 554, 770
HpyCH4V TGCA 3 cut(s) 11, 277, 380
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 177
HpyF3I CTNAG 1 cut(s) 15
HpySE526I ACGT 3 cut(s) 541, 554, 770
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 1 cut(s) 538
HspAI GCGC 2 cut(s) 180, 644
Kzo9I GATC 2 cut(s) 366, 475
LmnI GCTCC 4 cut(s) 28, 93, 98, 590
LpnPI CCDG 9 cut(s) 42, 138, 224, 263, 285, 341, 572, 746, 795
Lsp1109I GCAGC 1 cut(s) 392
LweI GCATC 2 cut(s) 20, 318
MaeI CTAG 1 cut(s) 669
MaeII ACGT 3 cut(s) 541, 554, 770
MaeIII GTNAC 2 cut(s) 351, 790
MalI GATC 2 cut(s) 368, 477
MboI GATC 2 cut(s) 366, 475
MboII GAAGA 4 cut(s) 304, 329, 536, 583
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 595, 749
MluCI AATT 7 cut(s) 374, 394, 485, 574, 599, 624, 740
MlyI GAGTC 3 cut(s) 47, 253, 291
MmeI TCCRAC 1 cut(s) 394
MnlI CCTC 5 cut(s) 10, 150, 630, 759, 791
Mph1103I ATGCAT 1 cut(s) 13
MroXI GAANNNNTTC 1 cut(s) 543
MseI TTAA 4 cut(s) 174, 261, 522, 802
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 1 cut(s) 211
MunI CAATTG 1 cut(s) 624
MvnI CGCG 2 cut(s) 242, 646
MwoI GCNNNNNNNGC 2 cut(s) 29, 177
NdeI CATATG 2 cut(s) 249, 640
NdeII GATC 2 cut(s) 366, 475
NlaIII CATG 1 cut(s) 538
NlaIV GGNNCC 1 cut(s) 73
NmuCI GTSAC 1 cut(s) 351
NsiI ATGCAT 1 cut(s) 13
NspV TTCGAA 2 cut(s) 315, 483
PcsI WCGNNNNNNNCGW 2 cut(s) 471, 480
PdmI GAANNNNTTC 1 cut(s) 543
PkrI GCNGC 3 cut(s) 34, 241, 382
PleI GAGTC 3 cut(s) 47, 252, 290
PpsI GAGTC 3 cut(s) 47, 252, 290
PsiI TTATAA 1 cut(s) 611
Psp124BI GAGCTC 2 cut(s) 595, 749
PspFI CCCAGC 1 cut(s) 586
PspN4I GGNNCC 1 cut(s) 73
PstI CTGCAG 1 cut(s) 279
PsuI RGATCY 1 cut(s) 366
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
SacI GAGCTC 2 cut(s) 595, 749
SalI GTCGAC 1 cut(s) 662
SaqAI TTAA 4 cut(s) 174, 261, 522, 802
SatI GCNGC 3 cut(s) 33, 240, 381
Sau3AI GATC 2 cut(s) 366, 475
ScaI AGTACT 1 cut(s) 497
SchI GAGTC 3 cut(s) 47, 253, 291
SduI GDGCHC 2 cut(s) 595, 749
SfaNI GCATC 2 cut(s) 20, 318
SfcI CTRYAG 1 cut(s) 275
SfuI TTCGAA 2 cut(s) 315, 483
Sse9I AATT 7 cut(s) 374, 394, 485, 574, 599, 624, 740
SsiI CCGC 6 cut(s) 26, 32, 240, 383, 527, 736
SspMI CTAG 1 cut(s) 669
SstI GAGCTC 2 cut(s) 595, 749
TaaI ACNGT 2 cut(s) 61, 433
TaiI ACGT 3 cut(s) 544, 557, 773
TaqI TCGA 3 cut(s) 315, 483, 663
TasI AATT 7 cut(s) 374, 394, 485, 574, 599, 624, 740
TatI WGTACW 1 cut(s) 495
TauI GCSGC 2 cut(s) 35, 242
Tru1I TTAA 4 cut(s) 174, 261, 522, 802
Tru9I TTAA 4 cut(s) 174, 261, 522, 802
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 1 cut(s) 351
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 1 cut(s) 351
TspDTI ATGAA 3 cut(s) 31, 442, 703
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 1 cut(s) 360
XapI RAATTY 3 cut(s) 394, 574, 599
XmiI GTMKAC 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 1 cut(s) 669
ZrmI AGTACT 1 cut(s) 497
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.