RLG00000013139

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000448
Physical Location & Seq
Forward (+)
26700520 .. 26702214
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013139

Sequence Viewer

Length: 978 bp
ATGGCTGTTCTTAGTTTAGCAGCAGCAGCTAAGCCAGTACTATTGCCACCAATTTTCATACTAGGTGATTCAACTGCTGATATAGGAACCAATAACTTCTTGCCAGGTAGCAAGGCAAGGGCAGACTTTCCCCACAATGGCATTGACTTTCCTAACTCACCAAGACCCACTGGAAGGTTCAGCAATGGCCTCAACAGTGCTGATTTTCTAGATCGTGCTACTTCTCTTCAAAAGAAGAAGTTCAGAGGTATTAACTTTGCTTCTGGAGGGTCTGGTCTGACCAGGAATGTTATTGGTGCCTCATTCAACAACCAAAAAAATGTTATCCTATTAACAGAACAGATACGGCAATTGTCATCCGTCAAGAACAATCTCATGGCCAAAATGGTCTTGATCTTCATCAGCAGTGGAAGCAATGACCTTTTCGGATATTATCATTCAAACAGTTCAATTCCCAAAGAAGAATTCTTGTCATCTTTAGTACTTGTTTATGAGAACCACTTGAAGACTTTATACACTCTTGGAGCAAGGAAGTTTGGAATCATTAGCGTCGCCCCAATTGGCTGCTGCCCATCTCAAAGGATTTTCAATGCTACTGGGGGATGTTTGGAGGAGTTGAATGATCATGCAATAGCCTTCCATTTAAAGCTGGATGCCCTATTGTGCAAGCTCAGCGCCGAACACGATGGCTTTAAGTACTCCCTTGGAAATTCATATGAAATGACAATAAATGTCATAAAGAACCCTCTTCCATTCAATTTTACACAAGTGGAAGCTGCATGTTGTGGAGCTGGGAAGCTCGATGGTGAATCCTTCTGTACACCAGATGCTAATCTATGTTCGAACCGCGACCAATACTTGTTCTGGGATCTATTCCATCCAACACAGGCGGCTTCTAAGTTGGCAGCTGTGATCCTCTACCATGGAGGACCACAATTTGTATCCCCAATTAACTTCGCTCAGTTACCCCAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

326

Amino Acids

35.24

Weight (kDa)

8.37

Isoelectric Point (pI)

25.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 18 - 303 2.8e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AccII CGCG 1 cut(s) 849
AciI CCGC 2 cut(s) 847, 890
AclWI GGATC 2 cut(s) 876, 907
AcoI YGGCCR 1 cut(s) 378
AcsI RAATTY 2 cut(s) 464, 709
AfaI GTAC 4 cut(s) 39, 483, 698, 820
AfiI CCNNNNNNNGG 2 cut(s) 137, 174
AgsI TTSAA 9 cut(s) 72, 230, 307, 441, 450, 505, 589, 619, 757
AjnI CCWGG 3 cut(s) 103, 281, 969
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AluBI AGCT 7 cut(s) 29, 649, 670, 776, 791, 799, 908
AluI AGCT 7 cut(s) 29, 649, 670, 776, 791, 799, 908
AlwI GGATC 2 cut(s) 876, 907
AoxI GGCC 2 cut(s) 187, 378
ApeKI GCWGC 7 cut(s) 20, 23, 26, 564, 567, 776, 905
ApoI RAATTY 2 cut(s) 464, 709
Asp700I GAANNNNTTC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 1 cut(s) 929
AsuHPI GGTGA 3 cut(s) 77, 150, 818
AsuII TTCGAA 1 cut(s) 842
AvaII GGWCC 1 cut(s) 929
BalI TGGCCA 1 cut(s) 380
BanI GGYRCC 1 cut(s) 296
BarI GAAGNNNNNNTAC 2 cut(s) 497, 529
BbsI GAAGAC 1 cut(s) 512
BbvI GCAGC 7 cut(s) 32, 35, 38, 551, 554, 763, 917
BccI CCATC 4 cut(s) 580, 680, 797, 885
BceAI ACGGC 1 cut(s) 362
BciT130I CCWGG 3 cut(s) 105, 283, 971
BciVI GTATCC 1 cut(s) 952
BclI TGATCA 1 cut(s) 622
BfaI CTAG 2 cut(s) 62, 209
BfoI RGCGCY 1 cut(s) 678
BfuI GTATCC 1 cut(s) 952
BisI GCNGC 8 cut(s) 21, 24, 27, 565, 568, 777, 891, 906
BlpI GCTNAGC 2 cut(s) 30, 671
BlsI GCNGC 8 cut(s) 22, 25, 28, 566, 569, 778, 892, 907
BmcAI AGTACT 3 cut(s) 39, 483, 698
Bme1390I CCNGG 3 cut(s) 105, 283, 971
Bme18I GGWCC 1 cut(s) 929
BmgT120I GGNCC 1 cut(s) 929
BmiI GGNNCC 2 cut(s) 88, 298
BmrFI CCNGG 3 cut(s) 105, 283, 971
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 2 cut(s) 643, 817
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 512
BpmI CTGGAG 1 cut(s) 285
Bpu1102I GCTNAGC 2 cut(s) 30, 671
Bpu14I TTCGAA 1 cut(s) 842
BsaBI GATNNNNATC 2 cut(s) 398, 831
BsaJI CCNNGG 3 cut(s) 703, 922, 969
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 174
Bse1I ACTGG 3 cut(s) 35, 175, 601
Bse3DI GCAATG 2 cut(s) 190, 421
Bse8I GATNNNNATC 2 cut(s) 398, 831
BseBI CCWGG 3 cut(s) 105, 283, 971
BseDI CCNNGG 3 cut(s) 703, 922, 969
BseGI GGATG 4 cut(s) 356, 608, 658, 877
BseJI GATNNNNATC 2 cut(s) 398, 831
BseLI CCNNNNNNNGG 2 cut(s) 137, 174
BseMI GCAATG 2 cut(s) 190, 421
BseMII CTCAG 2 cut(s) 685, 974
BseNI ACTGG 3 cut(s) 35, 175, 601
BseRI GAGGAG 1 cut(s) 626
BseXI GCAGC 7 cut(s) 32, 35, 38, 551, 554, 763, 917
BseYI CCCAGC 1 cut(s) 791
Bsh1236I CGCG 1 cut(s) 849
BshFI GGCC 2 cut(s) 189, 380
BshNI GGYRCC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 137, 174
BsnI GGCC 2 cut(s) 189, 380
Bsp119I TTCGAA 1 cut(s) 842
Bsp1407I TGTACA 1 cut(s) 818
Bsp143I GATC 5 cut(s) 211, 393, 622, 868, 912
Bsp1720I GCTNAGC 2 cut(s) 30, 671
Bsp19I CCATGG 1 cut(s) 922
BspACI CCGC 2 cut(s) 847, 890
BspANI GGCC 2 cut(s) 189, 380
BspCNI CTCAG 2 cut(s) 684, 973
BspFNI CGCG 1 cut(s) 849
BspLI GGNNCC 2 cut(s) 88, 298
BspPI GGATC 2 cut(s) 876, 907
BspT104I TTCGAA 1 cut(s) 842
BspT107I GGYRCC 1 cut(s) 296
BsrDI GCAATG 2 cut(s) 190, 421
BsrGI TGTACA 1 cut(s) 818
BsrI ACTGG 3 cut(s) 35, 175, 601
BssECI CCNNGG 3 cut(s) 703, 922, 969
BssMI GATC 5 cut(s) 211, 393, 622, 868, 912
BssT1I CCWWGG 2 cut(s) 703, 922
Bst2UI CCWGG 3 cut(s) 105, 283, 971
Bst4CI ACNGT 2 cut(s) 197, 446
Bst6I CTCTTC 2 cut(s) 231, 753
BstAUI TGTACA 1 cut(s) 818
BstBI TTCGAA 1 cut(s) 842
BstC8I GCNNGC 1 cut(s) 668
BstDEI CTNAG 5 cut(s) 11, 30, 671, 897, 960
BstDSI CCRYGG 1 cut(s) 922
BstF5I GGATG 4 cut(s) 356, 608, 658, 877
BstFNI CGCG 1 cut(s) 849
BstH2I RGCGCY 1 cut(s) 678
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 5 cut(s) 214, 396, 625, 871, 915
BstMBI GATC 5 cut(s) 211, 393, 622, 868, 912
BstMWI GCNNNNNNNGC 3 cut(s) 26, 411, 672
BstNI CCWGG 3 cut(s) 105, 283, 971
BstNSI RCATGY 1 cut(s) 783
BstSCI CCNGG 3 cut(s) 103, 281, 969
BstUI CGCG 1 cut(s) 849
BstV1I GCAGC 7 cut(s) 32, 35, 38, 551, 554, 763, 917
BstV2I GAAGAC 1 cut(s) 512
BstX2I RGATCY 1 cut(s) 868
BstYI RGATCY 1 cut(s) 868
BsuI GTATCC 1 cut(s) 952
BsuRI GGCC 2 cut(s) 189, 380
BtgI CCRYGG 1 cut(s) 922
BtsCI GGATG 4 cut(s) 356, 608, 658, 877
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 3 cut(s) 168, 202, 412
Cac8I GCNNGC 1 cut(s) 668
CfoI GCGC 1 cut(s) 677
Cfr13I GGNCC 1 cut(s) 929
CseI GACGC 1 cut(s) 538
Csp6I GTAC 4 cut(s) 38, 482, 697, 819
CviAII CATG 4 cut(s) 376, 626, 780, 923
CviQI GTAC 4 cut(s) 38, 482, 697, 819
DdeI CTNAG 5 cut(s) 11, 30, 671, 897, 960
DpnI GATC 5 cut(s) 213, 395, 624, 870, 914
DpnII GATC 5 cut(s) 211, 393, 622, 868, 912
DraI TTTAAA 1 cut(s) 645
EaeI YGGCCR 1 cut(s) 378
Eam1104I CTCTTC 2 cut(s) 231, 753
EarI CTCTTC 2 cut(s) 231, 753
Eco130I CCWWGG 2 cut(s) 703, 922
Eco47I GGWCC 1 cut(s) 929
EcoRI GAATTC 1 cut(s) 464
EcoRII CCWGG 3 cut(s) 103, 281, 969
EcoT14I CCWWGG 2 cut(s) 703, 922
ErhI CCWWGG 2 cut(s) 703, 922
FaeI CATG 4 cut(s) 379, 629, 783, 926
FatI CATG 4 cut(s) 375, 625, 779, 922
FauNDI CATATG 1 cut(s) 715
FbaI TGATCA 1 cut(s) 622
Fnu4HI GCNGC 8 cut(s) 21, 24, 27, 565, 568, 777, 891, 906
FokI GGATG 4 cut(s) 343, 615, 665, 864
Fsp4HI GCNGC 8 cut(s) 21, 24, 27, 565, 568, 777, 891, 906
FspBI CTAG 2 cut(s) 62, 209
GlaI GCGC 1 cut(s) 676
GluI GCNGC 8 cut(s) 21, 24, 27, 565, 568, 777, 891, 906
GsaI CCCAGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 285
HaeII RGCGCY 1 cut(s) 678
HaeIII GGCC 2 cut(s) 189, 380
HgaI GACGC 1 cut(s) 538
HhaI GCGC 1 cut(s) 677
Hin1II CATG 4 cut(s) 379, 629, 783, 926
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HinfI GANTC 3 cut(s) 68, 540, 809
HphI GGTGA 3 cut(s) 77, 150, 818
Hpy166II GTNNAC 1 cut(s) 821
Hpy188I TCNGA 3 cut(s) 245, 279, 428
Hpy188III TCNNGA 4 cut(s) 209, 264, 364, 391
Hpy8I GTNNAC 1 cut(s) 821
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 3 cut(s) 168, 646, 823
HpyCH4III ACNGT 2 cut(s) 197, 446
HpyCH4V TGCA 3 cut(s) 629, 666, 779
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 411, 672
HpyF3I CTNAG 5 cut(s) 11, 30, 671, 897, 960
Hsp92II CATG 4 cut(s) 379, 629, 783, 926
HspAI GCGC 1 cut(s) 675
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 5 cut(s) 211, 393, 622, 868, 912
LmnI GCTCC 2 cut(s) 524, 788
Lsp1109I GCAGC 7 cut(s) 32, 35, 38, 551, 554, 763, 917
LweI GCATC 2 cut(s) 643, 817
MaeI CTAG 2 cut(s) 62, 209
MaeIII GTNAC 1 cut(s) 963
MalI GATC 5 cut(s) 213, 395, 624, 870, 914
MboI GATC 5 cut(s) 211, 393, 622, 868, 912
MboII GAAGA 6 cut(s) 218, 247, 388, 473, 517, 740
MfeI CAATTG 2 cut(s) 350, 558
MflI RGATCY 1 cut(s) 868
MlsI TGGCCA 1 cut(s) 380
MluCI AATT 9 cut(s) 51, 350, 450, 464, 558, 709, 757, 935, 948
MluNI TGGCCA 1 cut(s) 380
MmeI TCCRAC 1 cut(s) 905
MnlI CCTC 8 cut(s) 200, 239, 260, 310, 604, 756, 920, 926
Mox20I TGGCCA 1 cut(s) 380
MroXI GAANNNNTTC 1 cut(s) 239
MscI TGGCCA 1 cut(s) 380
MseI TTAA 6 cut(s) 252, 332, 644, 693, 951, 976
Msp20I TGGCCA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 908
MspR9I CCNGG 3 cut(s) 105, 283, 971
MunI CAATTG 2 cut(s) 350, 558
MvaI CCWGG 3 cut(s) 105, 283, 971
MvnI CGCG 1 cut(s) 849
MwoI GCNNNNNNNGC 3 cut(s) 26, 411, 672
NcoI CCATGG 1 cut(s) 922
NdeI CATATG 1 cut(s) 715
NdeII GATC 5 cut(s) 211, 393, 622, 868, 912
NlaIII CATG 4 cut(s) 379, 629, 783, 926
NlaIV GGNNCC 2 cut(s) 88, 298
NspI RCATGY 1 cut(s) 783
NspV TTCGAA 1 cut(s) 842
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 3 cut(s) 68, 540, 809
PkrI GCNGC 8 cut(s) 22, 25, 28, 566, 569, 778, 892, 907
Psp6I CCWGG 3 cut(s) 103, 281, 969
PspFI CCCAGC 1 cut(s) 791
PspGI CCWGG 3 cut(s) 103, 281, 969
PspN4I GGNNCC 2 cut(s) 88, 298
PspPI GGNCC 1 cut(s) 929
PsuI RGATCY 1 cut(s) 868
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 4 cut(s) 39, 483, 698, 820
RsaNI GTAC 4 cut(s) 38, 482, 697, 819
SaqAI TTAA 6 cut(s) 252, 332, 644, 693, 951, 976
SatI GCNGC 8 cut(s) 21, 24, 27, 565, 568, 777, 891, 906
Sau3AI GATC 5 cut(s) 211, 393, 622, 868, 912
Sau96I GGNCC 1 cut(s) 929
ScaI AGTACT 3 cut(s) 39, 483, 698
ScrFI CCNGG 3 cut(s) 105, 283, 971
SfaNI GCATC 2 cut(s) 643, 817
SfuI TTCGAA 1 cut(s) 842
SinI GGWCC 1 cut(s) 929
Sse9I AATT 9 cut(s) 51, 350, 450, 464, 558, 709, 757, 935, 948
SsiI CCGC 2 cut(s) 847, 890
SspMI CTAG 2 cut(s) 62, 209
StyD4I CCNGG 3 cut(s) 103, 281, 969
StyI CCWWGG 2 cut(s) 703, 922
TaaI ACNGT 2 cut(s) 197, 446
TaqI TCGA 2 cut(s) 801, 842
TasI AATT 9 cut(s) 51, 350, 450, 464, 558, 709, 757, 935, 948
TatI WGTACW 4 cut(s) 37, 481, 696, 818
TauI GCSGC 1 cut(s) 893
TfiI GAWTC 3 cut(s) 68, 540, 809
Tru1I TTAA 6 cut(s) 252, 332, 644, 693, 951, 976
Tru9I TTAA 6 cut(s) 252, 332, 644, 693, 951, 976
TscAI CASTG 3 cut(s) 175, 202, 412
TseI GCWGC 7 cut(s) 20, 23, 26, 564, 567, 776, 905
TspDTI ATGAA 4 cut(s) 46, 388, 702, 732
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 3 cut(s) 175, 202, 412
VpaK11BI GGWCC 1 cut(s) 929
XapI RAATTY 2 cut(s) 464, 709
XbaI TCTAGA 1 cut(s) 208
XceI RCATGY 1 cut(s) 783
XmnI GAANNNNTTC 1 cut(s) 239
XspI CTAG 2 cut(s) 62, 209
ZrmI AGTACT 3 cut(s) 39, 483, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.