Rh2DG121100

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000448
Physical Location & Seq
Forward (+)
10088367 .. 10090737
2371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG121100.1

Sequence Viewer

Length: 816 bp
ATGGCCAATAACAATGGGTGGACACCCTTGTTTGCTGTTTGGCTTTTTGTGTTTGTATATCTGTCCATGCTAGGTTTCCATGCATCCTCTTCACAGCAAATTGTGCCTGCAATTTACATATTTGGAGATTCTCCAGGACATGTTGGAACCAATAATTACTTGCCTGATTGTACTGCAAGGGCTGATTACCTCTATAATGGGATTGACTACCCTCACTCTAAACCAACAGGAAGATTTAGCAACGGTTACAACGTCGTAGATTACATCGAGACCTTTGGCTACGCCACTGCCCACCACTACACTAGAATTTCCCACAAGGAGAGCCCGCCACCTTTTCTCTCTCTTTTAGAAGAGAAGAAGCAATTTCCTAACAGGAAGGTTCCAAATAAGGGAGTTAACTTTGCGTCAGGAGGATCAGGACTTCTGGATGATACTGGTAAACTACAGTGGGGAAATGTCGAGATCATTTCCTTGGGGGAACAAGTGCAGCAATTCCAAACTTTACGGAACAAAATCTCAGAATTATTGGGTGCTGGTGGATTGGCTAATATTTCTCAGTCTCTCTTTGTCATCAGCGTTGGAAGCAACGACATCTTTGAATTATTCGCTGCTAATAACCTGACTAAAAGAACCGAGCAAGAGTACTTGAGCATACTCATCTCCTCTTACGAAACTCTAAATAAGGGTTGGACACTGCTAAACTTATTTATGCTCGGAGCAAGGAAGTTTGGGATTATCAGCGTCTCACAAATTGGGTGCCGCCCTGGCCAGCGAAAGCTCAGTGAAAGAGGCGGGTGTTTGGAGGCCATGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

271

Amino Acids

30.02

Weight (kDa)

7.72

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 258 2.8e-12 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 756
AciI CCGC 3 cut(s) 326, 760, 792
AclWI GGATC 1 cut(s) 421
AcoI YGGCCR 2 cut(s) 3, 766
AcsI RAATTY 1 cut(s) 306
AfaI GTAC 2 cut(s) 172, 644
AfiI CCNNNNNNNGG 1 cut(s) 389
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 1 cut(s) 599
AjnI CCWGG 2 cut(s) 133, 763
AluBI AGCT 1 cut(s) 778
AluI AGCT 1 cut(s) 778
Alw26I GTCTC 3 cut(s) 263, 564, 748
AlwI GGATC 1 cut(s) 421
AoxI GGCC 3 cut(s) 3, 766, 804
ApeKI GCWGC 2 cut(s) 487, 608
ApoI RAATTY 1 cut(s) 306
BalI TGGCCA 2 cut(s) 5, 768
BanI GGYRCC 1 cut(s) 756
BanII GRGCYC 1 cut(s) 326
BbvI GCAGC 2 cut(s) 499, 595
BciT130I CCWGG 2 cut(s) 135, 765
BcoDI GTCTC 3 cut(s) 263, 564, 748
BfaI CTAG 2 cut(s) 71, 303
BfmI CTRYAG 1 cut(s) 443
BglI GCCNNNNNGGC 1 cut(s) 765
BisI GCNGC 3 cut(s) 488, 609, 760
BlsI GCNGC 3 cut(s) 489, 610, 761
BmcAI AGTACT 1 cut(s) 644
Bme1390I CCNGG 2 cut(s) 135, 765
BmiI GGNNCC 3 cut(s) 148, 381, 758
BmrFI CCNGG 2 cut(s) 135, 765
BmsI GCATC 1 cut(s) 92
BpmI CTGGAG 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 667
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 471, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 439
BseBI CCWGG 2 cut(s) 135, 765
BseDI CCNNGG 2 cut(s) 471, 763
BseGI GGATG 2 cut(s) 83, 433
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMII CTCAG 3 cut(s) 531, 569, 793
BseNI ACTGG 1 cut(s) 439
BseRI GAGGAG 1 cut(s) 652
BseXI GCAGC 2 cut(s) 499, 595
BsgI GTGCAG 1 cut(s) 506
BshFI GGCC 3 cut(s) 5, 768, 806
BshNI GGYRCC 1 cut(s) 756
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 3 cut(s) 263, 564, 748
BsmBI CGTCTC 1 cut(s) 748
BsnI GGCC 3 cut(s) 5, 768, 806
Bso31I GGTCTC 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 2 cut(s) 413, 462
BspACI CCGC 3 cut(s) 326, 760, 792
BspANI GGCC 3 cut(s) 5, 768, 806
BspCNI CTCAG 3 cut(s) 530, 568, 792
BspLI GGNNCC 3 cut(s) 148, 381, 758
BspPI GGATC 1 cut(s) 421
BspT107I GGYRCC 1 cut(s) 756
BspTNI GGTCTC 1 cut(s) 263
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 2 cut(s) 471, 763
BssMI GATC 2 cut(s) 413, 462
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 2 cut(s) 135, 765
Bst4CI ACNGT 2 cut(s) 245, 447
Bst6I CTCTTC 2 cut(s) 94, 345
BstAPI GCANNNNNTGC 1 cut(s) 103
BstC8I GCNNGC 3 cut(s) 108, 326, 770
BstDEI CTNAG 3 cut(s) 517, 555, 779
BstF5I GGATG 2 cut(s) 83, 433
BstKTI GATC 2 cut(s) 416, 465
BstMAI GTCTC 3 cut(s) 263, 564, 748
BstMBI GATC 2 cut(s) 413, 462
BstMWI GCNNNNNNNGC 3 cut(s) 103, 582, 765
BstNI CCWGG 2 cut(s) 135, 765
BstNSI RCATGY 1 cut(s) 143
BstSCI CCNGG 2 cut(s) 133, 763
BstSFI CTRYAG 1 cut(s) 443
BstV1I GCAGC 2 cut(s) 499, 595
BsuRI GGCC 3 cut(s) 5, 768, 806
BtsCI GGATG 2 cut(s) 83, 433
BtsI GCAGTG 2 cut(s) 285, 692
BtsIMutI CAGTG 4 cut(s) 285, 452, 692, 787
Cac8I GCNNGC 3 cut(s) 108, 326, 770
CseI GACGC 2 cut(s) 393, 730
Csp6I GTAC 2 cut(s) 171, 643
CviAII CATG 4 cut(s) 67, 80, 140, 808
CviJI RGCY 9 cut(s) 5, 43, 182, 279, 324, 545, 768, 778, 806
CviKI_1 RGCY 9 cut(s) 5, 43, 182, 279, 324, 545, 768, 778, 806
CviQI GTAC 2 cut(s) 171, 643
DdeI CTNAG 3 cut(s) 517, 555, 779
DpnI GATC 2 cut(s) 415, 464
DpnII GATC 2 cut(s) 413, 462
EaeI YGGCCR 2 cut(s) 3, 766
Eam1104I CTCTTC 2 cut(s) 94, 345
EarI CTCTTC 2 cut(s) 94, 345
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 133, 763
EcoT14I CCWWGG 1 cut(s) 471
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 471
Esp3I CGTCTC 1 cut(s) 748
FaeI CATG 4 cut(s) 70, 83, 143, 811
FaiI YATR 9 cut(s) 58, 68, 81, 119, 141, 195, 653, 710, 809
FatI CATG 4 cut(s) 66, 79, 139, 807
FauI CCCGC 2 cut(s) 333, 785
Fnu4HI GCNGC 3 cut(s) 488, 609, 760
FokI GGATG 2 cut(s) 70, 440
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 3 cut(s) 488, 609, 760
FspBI CTAG 2 cut(s) 71, 303
GluI GCNGC 3 cut(s) 488, 609, 760
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 3 cut(s) 5, 768, 806
HgaI GACGC 2 cut(s) 393, 730
Hin1II CATG 4 cut(s) 70, 83, 143, 811
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 1 cut(s) 128
HpaI GTTAAC 1 cut(s) 397
Hpy166II GTNNAC 3 cut(s) 21, 397, 440
Hpy188I TCNGA 2 cut(s) 520, 716
Hpy188III TCNNGA 5 cut(s) 268, 408, 417, 425, 460
Hpy8I GTNNAC 3 cut(s) 21, 397, 440
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 2 cut(s) 245, 447
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 4 cut(s) 83, 110, 176, 487
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 582, 765
HpyF3I CTNAG 3 cut(s) 517, 555, 779
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 4 cut(s) 70, 83, 143, 811
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 2 cut(s) 413, 462
LmnI GCTCC 1 cut(s) 716
Lsp1109I GCAGC 2 cut(s) 499, 595
LweI GCATC 1 cut(s) 92
MaeI CTAG 2 cut(s) 71, 303
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 245
MalI GATC 2 cut(s) 415, 464
MboI GATC 2 cut(s) 413, 462
MboII GAAGA 4 cut(s) 81, 243, 362, 367
MhlI GDGCHC 1 cut(s) 326
MlsI TGGCCA 2 cut(s) 5, 768
MluNI TGGCCA 2 cut(s) 5, 768
MmeI TCCRAC 3 cut(s) 124, 559, 668
MnlI CCTC 7 cut(s) 97, 200, 222, 404, 673, 782, 796
Mox20I TGGCCA 2 cut(s) 5, 768
Mph1103I ATGCAT 1 cut(s) 85
MscI TGGCCA 2 cut(s) 5, 768
MseI TTAA 2 cut(s) 396, 814
Msp20I TGGCCA 2 cut(s) 5, 768
MspR9I CCNGG 2 cut(s) 135, 765
MvaI CCWGG 2 cut(s) 135, 765
MwoI GCNNNNNNNGC 3 cut(s) 103, 582, 765
NdeII GATC 2 cut(s) 413, 462
NlaIII CATG 4 cut(s) 70, 83, 143, 811
NlaIV GGNNCC 3 cut(s) 148, 381, 758
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 1 cut(s) 143
PciI ACATGT 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 1 cut(s) 128
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 3 cut(s) 489, 610, 761
PscI ACATGT 1 cut(s) 139
Psp6I CCWGG 2 cut(s) 133, 763
PspGI CCWGG 2 cut(s) 133, 763
PspN4I GGNNCC 3 cut(s) 148, 381, 758
RsaI GTAC 2 cut(s) 172, 644
RsaNI GTAC 2 cut(s) 171, 643
SaqAI TTAA 2 cut(s) 396, 814
SatI GCNGC 3 cut(s) 488, 609, 760
Sau3AI GATC 2 cut(s) 413, 462
ScaI AGTACT 1 cut(s) 644
ScrFI CCNGG 2 cut(s) 135, 765
SduI GDGCHC 1 cut(s) 326
SetI ASST 8 cut(s) 76, 192, 255, 275, 334, 381, 621, 780
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 443
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SsiI CCGC 3 cut(s) 326, 760, 792
SspI AATATT 1 cut(s) 550
SspMI CTAG 2 cut(s) 71, 303
StyD4I CCNGG 2 cut(s) 133, 763
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 2 cut(s) 245, 447
TaiI ACGT 1 cut(s) 255
TaqI TCGA 2 cut(s) 267, 459
TatI WGTACW 2 cut(s) 170, 642
TauI GCSGC 1 cut(s) 762
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 2 cut(s) 396, 814
Tru9I TTAA 2 cut(s) 396, 814
TscAI CASTG 4 cut(s) 292, 452, 699, 787
TseI GCWGC 2 cut(s) 487, 608
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 4 cut(s) 292, 452, 699, 787
XapI RAATTY 1 cut(s) 306
XceI RCATGY 1 cut(s) 143
XspI CTAG 2 cut(s) 71, 303
ZrmI AGTACT 1 cut(s) 644
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.