Rorug02G0069700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000448
Physical Location & Seq
Forward (+)
5422280 .. 5422909
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0069700.1

Sequence Viewer

Length: 540 bp
ATGGGGAGCGAGAGCAACCTGAAGACATGGGCCAGTGTGGTAGACAAGCTTGTCGAATTAGGATTGTCCTCATCTACCGAAACAGGTGCATTTGCTGAAGAAATCTTTGCAAGAGTGTGTGGTAAAACATCTGGTTTAAAGCAATGCCAGATAAAGAAACGATTCAGGAAGAGGGCAATATTGGATGAGAAAGATGGAAATGATGATGATGGGGTGATTGCACAGGCGGAAGAGAAACGTGTTAAAAGGCGGAACTTGCCGGATAAAGGTGATCGTGCGAATGGTTCAGAGTCAGAAGAAGAAAGACTGCGTGATCAAAGAGAGAAAGAGCAATTGGAGCAAAATATAAGGAAGAGGGACGCAGCAGCCACGAGGAAAGTAGCAAAGAAAAATTTGAGACGAAAGGAGGAAGAGGAGGCTGTTCGAAAAACCAGTGCTGATATTGAAGGTTTAAGAAGAGCTTCGAGACAACAATATCTAAAGAAAAGAGTGCAAAAGAAACTGGATGATATAGAAGATGAGGACTACTTAATTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

179

Amino Acids

20.61

Weight (kDa)

9.26

Isoelectric Point (pI)

62.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 42
AciI CCGC 2 cut(s) 227, 250
AcsI RAATTY 1 cut(s) 391
AcuI CTGAAG 2 cut(s) 41, 117
AfiI CCNNNNNNNGG 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 1 cut(s) 446
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 2 cut(s) 49, 461
AluI AGCT 2 cut(s) 49, 461
Alw26I GTCTC 2 cut(s) 391, 460
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 2 cut(s) 362, 365
ApoI RAATTY 1 cut(s) 391
Asp700I GAANNNNTTC 2 cut(s) 161, 460
AspS9I GGNCC 1 cut(s) 30
AsuHPI GGTGA 2 cut(s) 226, 281
AsuII TTCGAA 1 cut(s) 424
BauI CACGAG 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 29
BbvI GCAGC 2 cut(s) 374, 377
BccI CCATC 2 cut(s) 188, 203
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 2 cut(s) 391, 460
BisI GCNGC 2 cut(s) 363, 366
BlsI GCNGC 2 cut(s) 364, 367
BmgT120I GGNCC 1 cut(s) 30
BpiI GAAGAC 1 cut(s) 29
Bpu14I TTCGAA 1 cut(s) 424
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse1I ACTGG 3 cut(s) 33, 432, 507
Bse3DI GCAATG 1 cut(s) 149
BseGI GGATG 2 cut(s) 190, 511
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMI GCAATG 1 cut(s) 149
BseNI ACTGG 3 cut(s) 33, 432, 507
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 2 cut(s) 374, 377
BshFI GGCC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 266
BsmAI GTCTC 2 cut(s) 391, 460
BsmBI CGTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 1 cut(s) 32
Bsp119I TTCGAA 1 cut(s) 424
Bsp143I GATC 2 cut(s) 271, 313
BspACI CCGC 2 cut(s) 227, 250
BspANI GGCC 1 cut(s) 32
BspQI GCTCTTC 1 cut(s) 451
BspT104I TTCGAA 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 149
BsrI ACTGG 3 cut(s) 33, 432, 507
BssMI GATC 2 cut(s) 271, 313
BssSI CACGAG 1 cut(s) 370
Bst2BI CACGAG 1 cut(s) 370
Bst6I CTCTTC 5 cut(s) 164, 225, 347, 405, 451
BstBI TTCGAA 1 cut(s) 424
BstF5I GGATG 2 cut(s) 190, 511
BstKTI GATC 2 cut(s) 274, 316
BstMAI GTCTC 2 cut(s) 391, 460
BstMBI GATC 2 cut(s) 271, 313
BstMWI GCNNNNNNNGC 2 cut(s) 256, 337
BstV1I GCAGC 2 cut(s) 374, 377
BstV2I GAAGAC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 32
BtsCI GGATG 2 cut(s) 190, 511
BtsIMutI CAGTG 2 cut(s) 40, 439
Cfr13I GGNCC 1 cut(s) 30
CseI GACGC 1 cut(s) 368
CviAII CATG 1 cut(s) 27
CviJI RGCY 5 cut(s) 32, 49, 368, 419, 461
CviKI_1 RGCY 5 cut(s) 32, 49, 368, 419, 461
DpnI GATC 2 cut(s) 273, 315
DpnII GATC 2 cut(s) 271, 313
DraI TTTAAA 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 50
Eam1104I CTCTTC 5 cut(s) 164, 225, 347, 405, 451
EarI CTCTTC 5 cut(s) 164, 225, 347, 405, 451
EciI GGCGGA 2 cut(s) 242, 265
Eco57I CTGAAG 2 cut(s) 41, 117
Esp3I CGTCTC 1 cut(s) 391
FaeI CATG 1 cut(s) 30
FaiI YATR 3 cut(s) 28, 347, 512
FalI AAGNNNNNCTT 2 cut(s) 445, 477
FaqI GGGAC 1 cut(s) 371
FatI CATG 1 cut(s) 26
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 363, 366
FokI GGATG 2 cut(s) 197, 518
Fsp4HI GCNGC 2 cut(s) 363, 366
GluI GCNGC 2 cut(s) 363, 366
HaeIII GGCC 1 cut(s) 32
HapII CCGG 1 cut(s) 260
HgaI GACGC 1 cut(s) 368
Hin1II CATG 1 cut(s) 30
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 162, 290
HpaII CCGG 1 cut(s) 260
HphI GGTGA 2 cut(s) 226, 281
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 289, 295
Hpy188III TCNNGA 2 cut(s) 166, 465
Hpy8I GTNNAC 1 cut(s) 43
HpyAV CCTTC 1 cut(s) 440
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 4 cut(s) 89, 110, 221, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 337
HpySE526I ACGT 1 cut(s) 238
Hsp92II CATG 1 cut(s) 30
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 2 cut(s) 271, 313
LguI GCTCTTC 1 cut(s) 451
LmnI GCTCC 2 cut(s) 6, 337
Lsp1109I GCAGC 2 cut(s) 374, 377
MaeII ACGT 1 cut(s) 238
MalI GATC 2 cut(s) 273, 315
MboI GATC 2 cut(s) 271, 313
MfeI CAATTG 1 cut(s) 332
MluCI AATT 4 cut(s) 56, 332, 391, 531
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 8 cut(s) 79, 165, 348, 366, 400, 406, 409, 514
MroXI GAANNNNTTC 2 cut(s) 161, 460
MseI TTAA 5 cut(s) 137, 243, 452, 530, 538
MspI CCGG 1 cut(s) 260
MunI CAATTG 1 cut(s) 332
MwoI GCNNNNNNNGC 2 cut(s) 256, 337
NdeII GATC 2 cut(s) 271, 313
NlaIII CATG 1 cut(s) 30
NspV TTCGAA 1 cut(s) 424
PciSI GCTCTTC 1 cut(s) 451
PdmI GAANNNNTTC 2 cut(s) 161, 460
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 2 cut(s) 364, 367
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 451
SaqAI TTAA 5 cut(s) 137, 243, 452, 530, 538
SatI GCNGC 2 cut(s) 363, 366
Sau3AI GATC 2 cut(s) 271, 313
Sau96I GGNCC 1 cut(s) 30
SchI GAGTC 1 cut(s) 299
SetI ASST 7 cut(s) 21, 51, 88, 241, 271, 451, 463
SfuI TTCGAA 1 cut(s) 424
Sse9I AATT 4 cut(s) 56, 332, 391, 531
SsiI CCGC 2 cut(s) 227, 250
SspI AATATT 1 cut(s) 180
TaiI ACGT 1 cut(s) 241
TaqI TCGA 3 cut(s) 54, 424, 464
TasI AATT 4 cut(s) 56, 332, 391, 531
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 5 cut(s) 137, 243, 452, 530, 538
Tru9I TTAA 5 cut(s) 137, 243, 452, 530, 538
TscAI CASTG 2 cut(s) 40, 439
TseI GCWGC 2 cut(s) 362, 365
TspRI CASTG 2 cut(s) 40, 439
XapI RAATTY 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 42
XmnI GAANNNNTTC 2 cut(s) 161, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.