RLG00000016747

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000448
Physical Location & Seq
Forward (+)
9677562 .. 9678882
1321 bp
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UTR
Exon/CDS
Intron
RLM00000016747

Sequence Viewer

Length: 813 bp
ATGCTAGGTTTCCATGCATCCTCTTCACAGCAAATTGTGCCTGCAATTTACATATTTGGAGATTCTACAGGAGATGTTGGAACCAATAATTACTTGCCTAATTGTACTGCAAGGGCTGATATCCTCTATAATGGGATTGACTATCCTCACTCTAAACCAACAGGAAGATTTAGCAACGGTTACAACGTCGTAGATTACATCGCCCAGTTTCTGGGTTACAAGAAGAGCCCGCCACCTTTTCTCTCTCTTTTAGACGAGAAGAAGCAATTTCCTAACAGGAGGGTTCCAAATAAGGGAGTTAACTTTGCGTCAGGAGGATCAGGTCTTCTGGAAGATACTGGTAAACTACAGTGGGGAAATGTCATTTCCTTGGGGGAACAAGTGCAGCAATTCCAAACCGTGCGGAACAAAATTTCAGAATTATTGGGTGCTGGTGGATTGGCAAGTATTTCAAAGTCTCTCTTTATCCTCAGCATTGGAAGCAACGACATCTTTGAATTATTCGCTCCTAATACACTGACAGTGCATAGAATCCTCTCCAACCTATTTATGCTCGGAGCAAGGAAGTTTGGGATTATCAGCGTCTCACCGATTGGGTGCTGTCCTGTCCATCGAAAACTCAGTGAAAGAGGTGGGTGTTTGGAGGCCATGAATGCTTATGCACACTTGTTCTATACGTCACTCCAAGTCCTCTTGCAGAAGTTGAGCTTACAATGCAGAGGGATGATTTACGCACTTGGAGATACATATAAAATGACCCAAGTTATTATGGAAAATCCTCTTCCATTTGGTAATTATACGCATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

271

Amino Acids

29.67

Weight (kDa)

9.03

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 16 - 257 7.3e-20 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 211
AciI CCGC 2 cut(s) 230, 403
AclWI GGATC 1 cut(s) 325
AcsI RAATTY 1 cut(s) 411
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 2 cut(s) 211, 293
AgsI TTSAA 2 cut(s) 453, 497
AluBI AGCT 1 cut(s) 708
AluI AGCT 1 cut(s) 708
Alw26I GTCTC 2 cut(s) 462, 589
AlwI GGATC 1 cut(s) 325
AlwNI CAGNNNCTG 1 cut(s) 211
AoxI GGCC 1 cut(s) 645
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 579
BanII GRGCYC 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 317
BbvCI CCTCAGC 1 cut(s) 470
BbvI GCAGC 1 cut(s) 397
BccI CCATC 1 cut(s) 618
BcoDI GTCTC 2 cut(s) 462, 589
BfaI CTAG 1 cut(s) 5
BfmI CTRYAG 2 cut(s) 66, 347
BisI GCNGC 1 cut(s) 386
BlsI GCNGC 1 cut(s) 387
BmiI GGNNCC 2 cut(s) 82, 285
BmrI ACTGGG 1 cut(s) 199
BmsI GCATC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 199
BpiI GAAGAC 1 cut(s) 317
Bpu10I CCTNAGC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 293
Bse1I ACTGG 2 cut(s) 205, 343
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 17, 729
BseLI CCNNNNNNNGG 2 cut(s) 211, 293
BseMII CTCAG 2 cut(s) 484, 634
BseNI ACTGG 2 cut(s) 205, 343
BseXI GCAGC 1 cut(s) 397
BsgI GTGCAG 1 cut(s) 404
BshFI GGCC 1 cut(s) 647
BslI CCNNNNNNNGG 2 cut(s) 211, 293
BsmAI GTCTC 2 cut(s) 462, 589
BsmBI CGTCTC 1 cut(s) 589
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 1 cut(s) 647
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 1 cut(s) 317
BspACI CCGC 2 cut(s) 230, 403
BspANI GGCC 1 cut(s) 647
BspCNI CTCAG 2 cut(s) 483, 633
BspLI GGNNCC 2 cut(s) 82, 285
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 218
BsrI ACTGG 2 cut(s) 205, 343
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 4 cut(s) 179, 351, 400, 523
Bst6I CTCTTC 3 cut(s) 28, 218, 786
BstAPI GCANNNNNTGC 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 42, 230
BstDEI CTNAG 2 cut(s) 470, 620
BstF5I GGATG 2 cut(s) 17, 729
BstKTI GATC 1 cut(s) 320
BstMAI GTCTC 2 cut(s) 462, 589
BstMBI GATC 1 cut(s) 317
BstMWI GCNNNNNNNGC 4 cut(s) 37, 480, 653, 714
BstSFI CTRYAG 2 cut(s) 66, 347
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 1 cut(s) 317
BsuRI GGCC 1 cut(s) 647
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 17, 729
BtsIMutI CAGTG 4 cut(s) 356, 515, 528, 628
Cac8I GCNNGC 2 cut(s) 42, 230
CaiI CAGNNNCTG 1 cut(s) 211
CseI GACGC 2 cut(s) 297, 571
Csp6I GTAC 1 cut(s) 105
CviAII CATG 2 cut(s) 14, 649
CviJI RGCY 4 cut(s) 116, 228, 647, 708
CviKI_1 RGCY 4 cut(s) 116, 228, 647, 708
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 2 cut(s) 470, 620
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
Eam1104I CTCTTC 3 cut(s) 28, 218, 786
EarI CTCTTC 3 cut(s) 28, 218, 786
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 121
EcoRV GATATC 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 19
EcoT38I GRGCYC 1 cut(s) 230
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 2 cut(s) 17, 652
FalI AAGNNNNNCTT 4 cut(s) 446, 478, 692, 724
FatI CATG 2 cut(s) 13, 648
FauI CCCGC 1 cut(s) 237
Fnu4HI GCNGC 1 cut(s) 386
FokI GGATG 2 cut(s) 4, 736
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 386
FspBI CTAG 1 cut(s) 5
GluI GCNGC 1 cut(s) 386
HaeIII GGCC 1 cut(s) 647
HgaI GACGC 2 cut(s) 297, 571
Hin1II CATG 2 cut(s) 17, 652
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 2 cut(s) 62, 531
HpaI GTTAAC 1 cut(s) 301
HphI GGTGA 1 cut(s) 579
Hpy166II GTNNAC 2 cut(s) 301, 344
Hpy188I TCNGA 2 cut(s) 418, 557
Hpy188III TCNNGA 2 cut(s) 312, 329
Hpy8I GTNNAC 2 cut(s) 301, 344
Hpy99I CGWCG 1 cut(s) 191
HpyCH4III ACNGT 4 cut(s) 179, 351, 400, 523
HpyCH4IV ACGT 2 cut(s) 186, 677
HpyCH4V TGCA 8 cut(s) 17, 44, 110, 385, 526, 662, 697, 717
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 480, 653, 714
HpyF3I CTNAG 2 cut(s) 470, 620
HpySE526I ACGT 2 cut(s) 186, 677
Hsp92II CATG 2 cut(s) 17, 652
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 1 cut(s) 317
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 2 cut(s) 511, 557
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 1 cut(s) 26
MaeI CTAG 1 cut(s) 5
MaeII ACGT 2 cut(s) 186, 677
MaeIII GTNAC 3 cut(s) 179, 215, 678
MalI GATC 1 cut(s) 319
MboI GATC 1 cut(s) 317
MboII GAAGA 8 cut(s) 15, 177, 235, 271, 317, 344, 773, 797
MhlI GDGCHC 1 cut(s) 230
MmeI TCCRAC 2 cut(s) 58, 564
Mph1103I ATGCAT 1 cut(s) 19
MseI TTAA 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 658
MwoI GCNNNNNNNGC 4 cut(s) 37, 480, 653, 714
NdeII GATC 1 cut(s) 317
NlaIII CATG 2 cut(s) 17, 652
NlaIV GGNNCC 2 cut(s) 82, 285
NmuCI GTSAC 1 cut(s) 678
NsiI ATGCAT 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 658
PfeI GAWTC 2 cut(s) 62, 531
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 1 cut(s) 387
PspN4I GGNNCC 2 cut(s) 82, 285
PstNI CAGNNNCTG 1 cut(s) 211
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 386
Sau3AI GATC 1 cut(s) 317
SduI GDGCHC 1 cut(s) 230
SetI ASST 8 cut(s) 10, 189, 238, 325, 546, 634, 680, 710
SfaNI GCATC 1 cut(s) 26
SfcI CTRYAG 2 cut(s) 66, 347
SsiI CCGC 2 cut(s) 230, 403
SspMI CTAG 1 cut(s) 5
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 179, 351, 400, 523
TaiI ACGT 2 cut(s) 189, 680
TaqI TCGA 1 cut(s) 613
TatI WGTACW 1 cut(s) 104
TfiI GAWTC 2 cut(s) 62, 531
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 4 cut(s) 356, 522, 528, 628
TseFI GTSAC 1 cut(s) 678
TseI GCWGC 1 cut(s) 385
Tsp45I GTSAC 1 cut(s) 678
TspDTI ATGAA 1 cut(s) 665
TspRI CASTG 4 cut(s) 356, 522, 528, 628
Van91I CCANNNNNTGG 1 cut(s) 211
XapI RAATTY 1 cut(s) 411
XspI CTAG 1 cut(s) 5
Zsp2I ATGCAT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.