Rw2G009120

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000448
Physical Location & Seq
Forward (+)
9144629 .. 9149199
4571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009120.1

Sequence Viewer

Length: 1095 bp
ATGGCCAAGTCTGCAATATTTTCTCTTTGGCTCAATATTATTGTGTATCTCGGCATACTTGGATTGCACGTCTCACACTCTCAGCCATCTGTGCCACCACTTTACATATTTGGTGACTCCACAGCTGATGTTGGAACCAACAATTATTTGCCCAATTCCACTGCGAGGGCCGATTCCCTTTATAACGGGATCGATTTTCCTTTGCGTAAACCAACTGGGAGATTTAGCAACGGTTACAACACTATTGATTATCTAGCCCAGCTTCTGAAATTCTACGAAAGCCCGCCGCCTTTTCTCTCGTTTTCAGACAGCAATATTTCGCATTTTCTCATCAGGACTGTTCCAACGACGGGAATCAATTTTGCTTCAGGAGGCTCGGGACTTCTGGATTGTACAGGACGTAAGTATGGTGATTTCTTTTGGGAACAAGTCCAACAATTTTCAAGTGTCCGGAGCAATATTTCAGAATTACTGGGTGCGAGTGCCGTCACAAATATCTCCAAGTCTCTTTTCATTATCAGCGTTGGAAGCAATGACATCTTCGAACATTATGATGCTAACGATACTAGTACTGGGCAAAACTACATTACCACTCTCATCTCCACCTACGAAACTCATCTAAGGAATTTATATGAGCTAGGAGCCAGAAGATTTGGGATTATTAGTGTTGGGGCACTCGGGTGCTGTCCGATCCTACGTAGTAAATATGGAGGTTGTTGTTTGGAACCCATGAATAGGGATGCTCAGTTGTTCTATACTGCACTCCGAGACCTCTTGCAAAAGTTGAGTTCAGAGTGCAAAGGGTTGATGTATGCACTTGCAGATTCATATAGTATGTCAAAATATATTATCGACCACCCCAATAAATCTGTTTTTTCCGAGATTAAAAGAGCTTGCTGTGGAAAAGGAAAGTTGAACGCACATGAGGCATGTAAACCGAATTCGGATTTGTGTAAAGATCGCACAACCTACTTGTTTTGGGACCAGTACCATCCAACACAGGAGGCTTCTTCTCTCGCAGCCAATATTCTTTATACTGGTACCAAACCATTCGTGGTGCCGATGGGTTTTGGTGAATTGGCCCTTCTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

40.23

Weight (kDa)

7.05

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 342 4.5e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
Acc65I GGTACC 1 cut(s) 1040
AccB1I GGYRCC 2 cut(s) 1040, 1057
AccIII TCCGGA 1 cut(s) 450
AciI CCGC 2 cut(s) 284, 287
AclWI GGATC 2 cut(s) 197, 685
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 269, 625, 940
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 4 cut(s) 394, 571, 989, 1042
AfiI CCNNNNNNNGG 3 cut(s) 165, 350, 735
AgsI TTSAA 2 cut(s) 444, 916
AhlI ACTAGT 1 cut(s) 566
AjiI CACGTC 1 cut(s) 70
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AleI CACNNNNGTG 1 cut(s) 679
AluBI AGCT 4 cut(s) 125, 262, 637, 893
AluI AGCT 4 cut(s) 125, 262, 637, 893
Alw26I GTCTC 3 cut(s) 76, 510, 762
AlwI GGATC 2 cut(s) 197, 685
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 2 cut(s) 376, 677
Aor13HI TCCGGA 1 cut(s) 450
AoxI GGCC 3 cut(s) 3, 168, 1080
ApeKI GCWGC 1 cut(s) 1019
ApoI RAATTY 3 cut(s) 269, 625, 940
Asp718I GGTACC 1 cut(s) 1040
AspS9I GGNCC 3 cut(s) 168, 982, 1081
AsuHPI GGTGA 3 cut(s) 125, 422, 1085
AsuII TTCGAA 1 cut(s) 543
AvaI CYCGRG 2 cut(s) 376, 677
AvaII GGWCC 1 cut(s) 982
BaeGI GKGCMC 1 cut(s) 676
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 1040, 1057
BbvI GCAGC 1 cut(s) 1031
BccI CCATC 3 cut(s) 94, 999, 1057
BceAI ACGGC 1 cut(s) 470
BcoDI GTCTC 3 cut(s) 76, 510, 762
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 3 cut(s) 254, 567, 638
BisI GCNGC 2 cut(s) 287, 1020
BlsI GCNGC 2 cut(s) 288, 1021
BmcAI AGTACT 1 cut(s) 571
Bme18I GGWCC 1 cut(s) 982
BmeT110I CYCGRG 2 cut(s) 376, 677
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 168, 982, 1081
BmiI GGNNCC 6 cut(s) 136, 643, 726, 983, 1042, 1059
BmrI ACTGGG 3 cut(s) 225, 482, 582
BmsI GCATC 2 cut(s) 544, 730
BmuI ACTGGG 3 cut(s) 225, 482, 582
Bpu14I TTCGAA 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 698
BsaI GGTCTC 1 cut(s) 762
BsaWI WCCGGW 1 cut(s) 450
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 350, 735
Bse1I ACTGG 5 cut(s) 220, 477, 577, 985, 1042
Bse3DI GCAATG 1 cut(s) 538
BseAI TCCGGA 1 cut(s) 450
BseCI ATCGAT 1 cut(s) 192
BseGI GGATG 2 cut(s) 745, 991
BseLI CCNNNNNNNGG 3 cut(s) 165, 350, 735
BseMI GCAATG 1 cut(s) 538
BseMII CTCAG 2 cut(s) 95, 758
BseNI ACTGG 5 cut(s) 220, 477, 577, 985, 1042
BseSI GKGCMC 1 cut(s) 676
BseXI GCAGC 1 cut(s) 1031
BseYI CCCAGC 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 744
BshFI GGCC 3 cut(s) 5, 170, 1082
BshNI GGYRCC 2 cut(s) 1040, 1057
BshVI ATCGAT 1 cut(s) 192
BsiHKCI CYCGRG 2 cut(s) 376, 677
BsiSI CCGG 1 cut(s) 451
BslFI GGGAC 2 cut(s) 393, 995
BslI CCNNNNNNNGG 3 cut(s) 165, 350, 735
BsmAI GTCTC 3 cut(s) 76, 510, 762
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 393, 995
BsnI GGCC 3 cut(s) 5, 170, 1082
Bso31I GGTCTC 1 cut(s) 762
BsoBI CYCGRG 2 cut(s) 376, 677
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 676
Bsp13I TCCGGA 1 cut(s) 450
Bsp1407I TGTACA 1 cut(s) 392
Bsp143I GATC 3 cut(s) 189, 690, 958
BspACI CCGC 2 cut(s) 284, 287
BspANI GGCC 3 cut(s) 5, 170, 1082
BspCNI CTCAG 2 cut(s) 94, 757
BspDI ATCGAT 1 cut(s) 192
BspEI TCCGGA 1 cut(s) 450
BspLI GGNNCC 6 cut(s) 136, 643, 726, 983, 1042, 1059
BspPI GGATC 2 cut(s) 197, 685
BspT104I TTCGAA 1 cut(s) 543
BspT107I GGYRCC 2 cut(s) 1040, 1057
BspTNI GGTCTC 1 cut(s) 762
BsrDI GCAATG 1 cut(s) 538
BsrGI TGTACA 1 cut(s) 392
BsrI ACTGG 5 cut(s) 220, 477, 577, 985, 1042
BssMI GATC 3 cut(s) 189, 690, 958
Bst4CI ACNGT 2 cut(s) 233, 340
BstAUI TGTACA 1 cut(s) 392
BstBAI YACGTR 1 cut(s) 698
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 2 cut(s) 284, 895
BstDEI CTNAG 3 cut(s) 81, 620, 744
BstF5I GGATG 2 cut(s) 745, 991
BstKTI GATC 3 cut(s) 192, 693, 961
BstMAI GTCTC 3 cut(s) 76, 510, 762
BstMBI GATC 3 cut(s) 189, 690, 958
BstMWI GCNNNNNNNGC 4 cut(s) 11, 91, 528, 926
BstNSI RCATGY 1 cut(s) 933
BstSLI GKGCMC 1 cut(s) 676
BstSNI TACGTA 1 cut(s) 698
BstV1I GCAGC 1 cut(s) 1031
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 3 cut(s) 5, 170, 1082
BsuTUI ATCGAT 1 cut(s) 192
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 2 cut(s) 745, 991
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 2 cut(s) 284, 895
CaiI CAGNNNCTG 1 cut(s) 265
Cfr13I GGNCC 3 cut(s) 168, 982, 1081
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 4 cut(s) 393, 570, 988, 1041
CviAII CATG 3 cut(s) 730, 923, 930
CviQI GTAC 4 cut(s) 393, 570, 988, 1041
DdeI CTNAG 3 cut(s) 81, 620, 744
DpnI GATC 3 cut(s) 191, 692, 960
DpnII GATC 3 cut(s) 189, 690, 958
EaeI YGGCCR 1 cut(s) 3
Eco105I TACGTA 1 cut(s) 698
Eco31I GGTCTC 1 cut(s) 762
Eco47I GGWCC 1 cut(s) 982
Eco57I CTGAAG 1 cut(s) 351
Eco88I CYCGRG 2 cut(s) 376, 677
EcoRI GAATTC 1 cut(s) 940
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 3 cut(s) 733, 926, 933
FaqI GGGAC 2 cut(s) 393, 995
FatI CATG 3 cut(s) 729, 922, 929
FauI CCCGC 1 cut(s) 291
Fnu4HI GCNGC 2 cut(s) 287, 1020
FokI GGATG 2 cut(s) 752, 978
Fsp4HI GCNGC 2 cut(s) 287, 1020
FspBI CTAG 3 cut(s) 254, 567, 638
GluI GCNGC 2 cut(s) 287, 1020
GsaI CCCAGC 1 cut(s) 262
HaeIII GGCC 3 cut(s) 5, 170, 1082
HapII CCGG 1 cut(s) 451
Hin1II CATG 3 cut(s) 733, 926, 933
HinfI GANTC 4 cut(s) 116, 173, 354, 824
HpaII CCGG 1 cut(s) 451
HphI GGTGA 3 cut(s) 125, 422, 1085
Hpy166II GTNNAC 2 cut(s) 209, 935
Hpy188I TCNGA 8 cut(s) 267, 307, 466, 690, 767, 793, 880, 946
Hpy188III TCNNGA 5 cut(s) 334, 369, 378, 386, 451
Hpy8I GTNNAC 2 cut(s) 209, 935
Hpy99I CGWCG 1 cut(s) 352
HpyAV CCTTC 1 cut(s) 1094
HpyCH4III ACNGT 2 cut(s) 233, 340
HpyCH4IV ACGT 3 cut(s) 69, 400, 697
HpyCH4V TGCA 7 cut(s) 14, 67, 761, 778, 798, 815, 821
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 91, 528, 926
HpyF3I CTNAG 3 cut(s) 81, 620, 744
HpySE526I ACGT 3 cut(s) 69, 400, 697
Hsp92II CATG 3 cut(s) 733, 926, 933
Kpn2I TCCGGA 1 cut(s) 450
KpnI GGTACC 1 cut(s) 1044
Kzo9I GATC 3 cut(s) 189, 690, 958
LmnI GCTCC 2 cut(s) 453, 641
Lsp1109I GCAGC 1 cut(s) 1031
LweI GCATC 2 cut(s) 544, 730
MaeI CTAG 3 cut(s) 254, 567, 638
MaeII ACGT 3 cut(s) 69, 400, 697
MaeIII GTNAC 3 cut(s) 113, 233, 487
MalI GATC 3 cut(s) 191, 692, 960
MboI GATC 3 cut(s) 189, 690, 958
MboII GAAGA 3 cut(s) 532, 660, 1002
MhlI GDGCHC 1 cut(s) 676
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 142, 154, 269, 358, 437, 467, 625, 940, 1076
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 5 cut(s) 112, 368, 457, 505, 1019
MnlI CCTC 6 cut(s) 159, 365, 704, 782, 919, 997
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 450
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 885
MslI CAYNNNNRTG 2 cut(s) 552, 679
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 1 cut(s) 451
MwoI GCNNNNNNNGC 4 cut(s) 11, 91, 528, 926
NdeII GATC 3 cut(s) 189, 690, 958
NlaIII CATG 3 cut(s) 733, 926, 933
NlaIV GGNNCC 6 cut(s) 136, 643, 726, 983, 1042, 1059
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 113, 487
NspI RCATGY 1 cut(s) 933
NspV TTCGAA 1 cut(s) 543
OliI CACNNNNGTG 1 cut(s) 679
PfeI GAWTC 3 cut(s) 173, 354, 824
PkrI GCNGC 2 cut(s) 288, 1021
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
Ppu21I YACGTR 1 cut(s) 698
PsiI TTATAA 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 258
PspN4I GGNNCC 6 cut(s) 136, 643, 726, 983, 1042, 1059
PspPI GGNCC 3 cut(s) 168, 982, 1081
PstNI CAGNNNCTG 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 4 cut(s) 394, 571, 989, 1042
RsaNI GTAC 4 cut(s) 393, 570, 988, 1041
RseI CAYNNNNRTG 2 cut(s) 552, 679
SaqAI TTAA 1 cut(s) 885
SatI GCNGC 2 cut(s) 287, 1020
Sau3AI GATC 3 cut(s) 189, 690, 958
Sau96I GGNCC 3 cut(s) 168, 982, 1081
ScaI AGTACT 1 cut(s) 571
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 2 cut(s) 544, 730
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 1 cut(s) 982
SmiMI CAYNNNNRTG 2 cut(s) 552, 679
SnaBI TACGTA 1 cut(s) 698
SpeI ACTAGT 1 cut(s) 566
Sse9I AATT 9 cut(s) 142, 154, 269, 358, 437, 467, 625, 940, 1076
SsiI CCGC 2 cut(s) 284, 287
SspI AATATT 5 cut(s) 18, 37, 316, 460, 1027
SspMI CTAG 3 cut(s) 254, 567, 638
TaaI ACNGT 2 cut(s) 233, 340
TaiI ACGT 3 cut(s) 72, 403, 700
TaqI TCGA 3 cut(s) 192, 543, 852
TasI AATT 9 cut(s) 142, 154, 269, 358, 437, 467, 625, 940, 1076
TatI WGTACW 2 cut(s) 392, 569
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 3 cut(s) 173, 354, 824
Tru1I TTAA 1 cut(s) 885
Tru9I TTAA 1 cut(s) 885
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 2 cut(s) 113, 487
TseI GCWGC 1 cut(s) 1019
Tsp45I GTSAC 2 cut(s) 113, 487
TspDTI ATGAA 3 cut(s) 502, 746, 816
TspRI CASTG 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 982
XapI RAATTY 3 cut(s) 269, 625, 940
XceI RCATGY 1 cut(s) 933
XcmI CCANNNNNNNNNTGG 1 cut(s) 1051
XspI CTAG 3 cut(s) 254, 567, 638
ZrmI AGTACT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.