FvH4_2g18320

Zinc-binding dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000313
Physical Location & Seq
Forward (+)
15706655 .. 15708921
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g18320.t1

Sequence Viewer

Length: 999 bp
ATGGCAGCCATGGCGGCGAAACTCATGCATGCGGTTCAGTACGACGCTTATGGCGGAGGACCTTCTGGTCTCAAGCATGTTGAGGTTCCAATCCCAACTCCGAAGAAAGATGAGGTTTTGCTGAAATTGGAAGCCGCTAGCATAAACCCAGTTGATTGGAAAATTCAGAAAGGCGTAGCGCGCCCTCTTTTCCCACGCAAATTTCCTTATATACCTGTTACTGATGTGGCCGGAGAGGTTGTGGAGGTTGGACAAGGAGTTCAGAAGTTCAAAGCAGGCGACAAAGTTGTGGCATATCTCCACCTTGTTAATGGAGGTGGATTTGCTGAATTTGCAACTGCTAGTGAGAGTTTGACGGTTGCTAGGCCGCCTGAAGTTTCAGCAGCTGAAGCTGCAGGATTACCTGTTGCTGGTCTCACAGCTCACCAATGTCTCACCCAAGCAGCAGGAGTCAAGCTTGACGGCACTGGCCCGCAAAAGAACATATTGGTTACTGCTGCTTCTGGTGGTGTGGGTCAGTATGCAGTCCAACTTGCAAAACTGGGGAACACTCATGTCACAGCTACTTGTGGATCTCGTAACATTGATTTTGTAAAGAGCTTAGGAGCTGATGAGGTTCTTGACTACAAGACCCCTGAAGGGGCAGCTTTGAAGAGCCCATCTGGTCGCAAGTATGATGCTGTTATCCACTGTGCAACCGGCATTCCTTGGTGCACTTTTGAGCCGAATTTGAGTCCAAATGGGAAGGTTATAGACATTACTCCGGGTCCAACTGCTTTGCTCACTTCTGCTCTAAAGAAACTCACCTTCTCCAAGAAGAAGCTGGTGCCGCTACTCATGAATGCCAAGGCAGAGAATCTGGATTATCTTGTGAAATTGGTGAAGGAAGGAAAGCTGAGGACAGTGATCGACTCCAAATATCCTCTGAGCAAGGCTGAAGAAGCTTGGGCTAAGAGTATTGAAGGCCATGCTACGGGGAAGATCATTGTGGAGCCTTAA

Protein Analysis

333

Amino Acids

34.94

Weight (kDa)

9.03

Isoelectric Point (pI)

26.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 121 5.2e-16 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 170 - 251 4.2e-10 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 201 - 330 3.1e-23 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13010
fragaria_vesca FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 66
AccB1I GGYRCC 1 cut(s) 826
AccII CGCG 1 cut(s) 181
AciI CCGC 7 cut(s) 14, 32, 54, 135, 368, 473, 830
AclWI GGATC 1 cut(s) 580
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 4 cut(s) 162, 200, 329, 727
AcuI CTGAAG 4 cut(s) 393, 408, 657, 957
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 4 cut(s) 410, 639, 640, 973
AgsI TTSAA 3 cut(s) 271, 652, 962
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
Alw21I GWGCWC 1 cut(s) 716
Alw26I GTCTC 3 cut(s) 74, 419, 437
Alw44I GTGCAC 1 cut(s) 712
AlwI GGATC 1 cut(s) 580
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 4 cut(s) 228, 365, 469, 964
ApaLI GTGCAC 1 cut(s) 712
ApeKI GCWGC 6 cut(s) 5, 383, 392, 443, 497, 644
ApoI RAATTY 4 cut(s) 162, 200, 329, 727
AspLEI GCGC 2 cut(s) 181, 183
AspS9I GGNCC 3 cut(s) 59, 470, 767
AsuC2I CCSGG 1 cut(s) 765
AsuHPI GGTGA 4 cut(s) 416, 427, 796, 892
AsuNHI GCTAGC 1 cut(s) 137
AvaII GGWCC 2 cut(s) 59, 767
BaeGI GKGCMC 1 cut(s) 716
BanI GGYRCC 1 cut(s) 826
BanII GRGCYC 1 cut(s) 659
Bbv12I GWGCWC 1 cut(s) 716
BbvCI CCTCAGC 1 cut(s) 896
BbvI GCAGC 6 cut(s) 17, 379, 395, 455, 484, 656
BccI CCATC 1 cut(s) 667
BceAI ACGGC 1 cut(s) 478
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BcnI CCSGG 1 cut(s) 765
BcoDI GTCTC 3 cut(s) 74, 419, 437
BfaI CTAG 3 cut(s) 138, 342, 363
BfmI CTRYAG 1 cut(s) 393
BglI GCCNNNNNGGC 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 765
Bme18I GGWCC 2 cut(s) 59, 767
BmgT120I GGNCC 3 cut(s) 59, 470, 767
BmiI GGNNCC 4 cut(s) 87, 768, 828, 993
BmrFI CCNGG 1 cut(s) 765
BmrI ACTGGG 2 cut(s) 143, 551
BmsI GCATC 1 cut(s) 667
BmtI GCTAGC 1 cut(s) 141
BmuI ACTGGG 2 cut(s) 143, 551
Bpu10I CCTNAGC 2 cut(s) 601, 896
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 765
BsaI GGTCTC 2 cut(s) 74, 419
BsaJI CCNNGG 3 cut(s) 9, 707, 846
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 4 cut(s) 410, 639, 640, 973
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 3 cut(s) 149, 472, 546
BseDI CCNNGG 3 cut(s) 9, 707, 846
BseLI CCNNNNNNNGG 4 cut(s) 410, 639, 640, 973
BseMII CTCAG 2 cut(s) 887, 917
BseNI ACTGG 3 cut(s) 149, 472, 546
BsePI GCGCGC 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 716
BseXI GCAGC 6 cut(s) 17, 379, 395, 455, 484, 656
Bsh1236I CGCG 1 cut(s) 181
BshFI GGCC 4 cut(s) 230, 367, 471, 966
BshNI GGYRCC 1 cut(s) 826
BsiHKAI GWGCWC 1 cut(s) 716
BsiSI CCGG 3 cut(s) 231, 699, 764
BslI CCNNNNNNNGG 4 cut(s) 410, 639, 640, 973
BsmAI GTCTC 3 cut(s) 74, 419, 437
BsmI GAATGC 2 cut(s) 702, 847
BsnI GGCC 4 cut(s) 230, 367, 471, 966
Bso31I GGTCTC 2 cut(s) 74, 419
Bsp1286I GDGCHC 2 cut(s) 659, 716
Bsp143I GATC 3 cut(s) 572, 906, 981
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 7 cut(s) 14, 32, 54, 135, 368, 473, 830
BspANI GGCC 4 cut(s) 230, 367, 471, 966
BspCNI CTCAG 2 cut(s) 888, 918
BspFNI CGCG 1 cut(s) 181
BspHI TCATGA 1 cut(s) 837
BspLI GGNNCC 4 cut(s) 87, 768, 828, 993
BspMAI CTGCAG 1 cut(s) 397
BspOI GCTAGC 1 cut(s) 141
BspPI GGATC 1 cut(s) 580
BspQI GCTCTTC 1 cut(s) 647
BspT107I GGYRCC 1 cut(s) 826
BspTNI GGTCTC 2 cut(s) 74, 419
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 3 cut(s) 149, 472, 546
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 3 cut(s) 9, 707, 846
BssHII GCGCGC 1 cut(s) 179
BssMI GATC 3 cut(s) 572, 906, 981
BssT1I CCWWGG 3 cut(s) 9, 707, 846
Bst4CI ACNGT 3 cut(s) 358, 692, 904
Bst6I CTCTTC 1 cut(s) 647
BstC8I GCNNGC 5 cut(s) 30, 139, 181, 277, 473
BstDEI CTNAG 4 cut(s) 601, 896, 926, 951
BstDSI CCRYGG 1 cut(s) 9
BstFNI CGCG 1 cut(s) 181
BstHHI GCGC 2 cut(s) 181, 183
BstKTI GATC 3 cut(s) 575, 909, 984
BstMAI GTCTC 3 cut(s) 74, 419, 437
BstMBI GATC 3 cut(s) 572, 906, 981
BstMWI GCNNNNNNNGC 8 cut(s) 11, 14, 180, 332, 389, 392, 829, 941
BstNSI RCATGY 2 cut(s) 32, 80
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 1 cut(s) 393
BstSLI GKGCMC 1 cut(s) 716
BstUI CGCG 1 cut(s) 181
BstV1I GCAGC 6 cut(s) 17, 379, 395, 455, 484, 656
BstX2I RGATCY 1 cut(s) 572
BstXI CCANNNNNNTGG 1 cut(s) 156
BstYI RGATCY 1 cut(s) 572
BsuRI GGCC 4 cut(s) 230, 367, 471, 966
BtgI CCRYGG 1 cut(s) 9
BtsIMutI CAGTG 3 cut(s) 465, 688, 909
Cac8I GCNNGC 5 cut(s) 30, 139, 181, 277, 473
CaiI CAGNNNCTG 1 cut(s) 386
CciI TCATGA 1 cut(s) 837
CfoI GCGC 2 cut(s) 181, 183
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 3 cut(s) 59, 470, 767
CseI GACGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 40
CviAII CATG 7 cut(s) 10, 25, 29, 77, 554, 838, 968
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 4 cut(s) 601, 896, 926, 951
DpnI GATC 3 cut(s) 574, 908, 983
DpnII GATC 3 cut(s) 572, 906, 981
DrdI GACNNNNNNGTC 1 cut(s) 66
DseDI GACNNNNNNGTC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
EciI GGCGGA 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 9, 707, 846
Eco24I GRGCYC 1 cut(s) 659
Eco31I GGTCTC 2 cut(s) 74, 419
Eco47I GGWCC 2 cut(s) 59, 767
Eco57I CTGAAG 4 cut(s) 393, 408, 657, 957
EcoO109I RGGNCCY 1 cut(s) 59
EcoT14I CCWWGG 3 cut(s) 9, 707, 846
EcoT22I ATGCAT 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 3 cut(s) 9, 707, 846
FaeI CATG 7 cut(s) 13, 28, 32, 80, 557, 841, 971
FatI CATG 7 cut(s) 9, 24, 28, 76, 553, 837, 967
FauI CCCGC 1 cut(s) 480
FriOI GRGCYC 1 cut(s) 659
FspBI CTAG 3 cut(s) 138, 342, 363
GlaI GCGC 2 cut(s) 180, 182
HaeIII GGCC 4 cut(s) 230, 367, 471, 966
HapII CCGG 3 cut(s) 231, 699, 764
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 181, 183
Hin1II CATG 7 cut(s) 13, 28, 32, 80, 557, 841, 971
Hin6I GCGC 2 cut(s) 179, 181
HinP1I GCGC 2 cut(s) 179, 181
HindIII AAGCTT 2 cut(s) 455, 942
HinfI GANTC 4 cut(s) 450, 733, 856, 911
HpaII CCGG 3 cut(s) 231, 699, 764
HphI GGTGA 4 cut(s) 416, 427, 796, 892
Hpy166II GTNNAC 1 cut(s) 714
Hpy188I TCNGA 4 cut(s) 102, 168, 264, 927
Hpy188III TCNNGA 3 cut(s) 620, 838, 860
Hpy8I GTNNAC 1 cut(s) 714
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 7 cut(s) 72, 632, 739, 817, 877, 881, 956
HpyCH4III ACNGT 3 cut(s) 358, 692, 904
HpyCH4V TGCA 7 cut(s) 28, 335, 395, 524, 536, 695, 714
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 14, 180, 332, 389, 392, 829, 941
HpyF3I CTNAG 4 cut(s) 601, 896, 926, 951
Hsp92II CATG 7 cut(s) 13, 28, 32, 80, 557, 841, 971
HspAI GCGC 2 cut(s) 179, 181
Kzo9I GATC 3 cut(s) 572, 906, 981
LguI GCTCTTC 1 cut(s) 647
LmnI GCTCC 2 cut(s) 605, 991
Lsp1109I GCAGC 6 cut(s) 17, 379, 395, 455, 484, 656
LweI GCATC 1 cut(s) 667
MaeI CTAG 3 cut(s) 138, 342, 363
MaeIII GTNAC 4 cut(s) 217, 490, 556, 578
MalI GATC 3 cut(s) 574, 908, 983
MboI GATC 3 cut(s) 572, 906, 981
MboII GAAGA 5 cut(s) 115, 664, 829, 950, 991
MflI RGATCY 1 cut(s) 572
MhlI GDGCHC 2 cut(s) 659, 716
MluCI AATT 6 cut(s) 125, 162, 200, 329, 727, 875
MlyI GAGTC 3 cut(s) 459, 742, 905
MmeI TCCRAC 3 cut(s) 229, 553, 794
Mph1103I ATGCAT 1 cut(s) 30
MseI TTAA 2 cut(s) 309, 997
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 3 cut(s) 231, 699, 764
MspR9I CCNGG 1 cut(s) 765
Mva1269I GAATGC 2 cut(s) 702, 847
MvnI CGCG 1 cut(s) 181
MwoI GCNNNNNNNGC 8 cut(s) 11, 14, 180, 332, 389, 392, 829, 941
NciI CCSGG 1 cut(s) 765
NcoI CCATGG 1 cut(s) 9
NdeII GATC 3 cut(s) 572, 906, 981
NheI GCTAGC 1 cut(s) 137
NlaIII CATG 7 cut(s) 13, 28, 32, 80, 557, 841, 971
NlaIV GGNNCC 4 cut(s) 87, 768, 828, 993
NmuCI GTSAC 1 cut(s) 556
NsiI ATGCAT 1 cut(s) 30
NspI RCATGY 2 cut(s) 32, 80
PaeI GCATGC 1 cut(s) 32
PagI TCATGA 1 cut(s) 837
PauI GCGCGC 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 647
PctI GAATGC 2 cut(s) 702, 847
PfeI GAWTC 1 cut(s) 856
PleI GAGTC 3 cut(s) 458, 741, 905
PpsI GAGTC 3 cut(s) 458, 741, 905
PpuMI RGGWCCY 1 cut(s) 59
Psp5II RGGWCCY 1 cut(s) 59
PspN4I GGNNCC 4 cut(s) 87, 768, 828, 993
PspPI GGNCC 3 cut(s) 59, 470, 767
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 1 cut(s) 572
PteI GCGCGC 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SapI GCTCTTC 1 cut(s) 647
SaqAI TTAA 2 cut(s) 309, 997
Sau3AI GATC 3 cut(s) 572, 906, 981
Sau96I GGNCC 3 cut(s) 59, 470, 767
SchI GAGTC 3 cut(s) 459, 742, 905
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 2 cut(s) 659, 716
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 2 cut(s) 59, 767
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SphI GCATGC 1 cut(s) 32
Sse9I AATT 6 cut(s) 125, 162, 200, 329, 727, 875
SsiI CCGC 7 cut(s) 14, 32, 54, 135, 368, 473, 830
SspMI CTAG 3 cut(s) 138, 342, 363
StyD4I CCNGG 1 cut(s) 763
StyI CCWWGG 3 cut(s) 9, 707, 846
TaaI ACNGT 3 cut(s) 358, 692, 904
TaqI TCGA 1 cut(s) 909
TasI AATT 6 cut(s) 125, 162, 200, 329, 727, 875
TauI GCSGC 4 cut(s) 17, 137, 370, 832
TfiI GAWTC 1 cut(s) 856
Tru1I TTAA 2 cut(s) 309, 997
Tru9I TTAA 2 cut(s) 309, 997
TscAI CASTG 3 cut(s) 472, 695, 909
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 6 cut(s) 5, 383, 392, 443, 497, 644
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 1 cut(s) 854
TspRI CASTG 3 cut(s) 472, 695, 909
VneI GTGCAC 1 cut(s) 712
VpaK11BI GGWCC 2 cut(s) 59, 767
XapI RAATTY 4 cut(s) 162, 200, 329, 727
XceI RCATGY 2 cut(s) 32, 80
XcmI CCANNNNNNNNNTGG 2 cut(s) 308, 820
XspI CTAG 3 cut(s) 138, 342, 363
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.