Rh6CG271000

Zinc-binding dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000313
Physical Location & Seq
Forward (+)
44567225 .. 44569954
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG271000.1

Sequence Viewer

Length: 999 bp
ATGGCAGCCATGGCGGCAAAACTTATGCGTGCGGTTCAGTACGACGGATATGGTGGAGGACCTTCCGGTCTAAAGCATGTTGAGGTTCCGATCCCAACTCCGAAGAAAGATGAGGTTTTGCTGAAATTGGAAGCGGCTACCATAAATCCAATTGATTGGAAAGTTCAGAAAGGCGTACTGCGCCCTTTTTTACCAAGAAAGTTCCCTTATATACCTGTTAGTGATGTGGCTGGAGAGGTTGTCGACGTTGGACAAGGAGTTCAGAAGTTCAAAGCGGGCGACAAAGTTGTAGCATTTTTCACCCAGGCTAATGGAGGTGGATTTGCTGAGTTTGCAACTGCTAGTGAGAGTTTGACCGTTACTAGGCCGCCTGAAGTTTCAGCAGCCGAAGGTGCAGGGTTACCTATTGCTGGTCTCACAGCTCACCAGTGTCTCACCCAAGCTGCAGGAGTCAAGCTTGATGGCACTGGCCCACAGAAGAACATATTGGTTACTGCTGCCTCAGGCGGTGTAGGTCACTATGCAGTTCAATTGGCAAAGCTTGGGAACACTCACGTGACAGCCACTTGTGGTGCACGCAACGTTGAATTTGTCAAGAGCTTAGGGGCCGATGAGGTTCTTGACTACAAGACCCCTGAAGGGGCAGCTCTGAATAGCCCTTCTGGTCGGAAGTACGATGCTGTTATCCACTGTGCAACTGGCATTCCTTGGTCCACATTTGAGCCGAATTTGAGTCCAAATGGGAAGGTTATAGACATTACTCCCAGTCCAAGTGCATTGCTTACTTTCGCTCTGAAGAAACTCACCTTCTCCAAAAAGCAACTGGTGCCGCTGTTCGTGAATGCCAAGGCAGAGAATCTGGATTGTCTTGTGAAGTTGGTGAAGGAAGGAAAGCTCAAGACAGTGATTGACTCAAAGTATCCTCTGAGCAAGGCTGAAGAAGCTTGGGCTAAGAGTATTGATGGCCATGCTACTGGGAAGATCATTGTGGAGCCTTAA

Protein Analysis

332

Amino Acids

34.91

Weight (kDa)

8.95

Isoelectric Point (pI)

25.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 121 3e-15 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 170 - 294 5.1e-11 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 201 - 330 3.1e-23 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13010
fragaria_vesca FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 66
AccB1I GGYRCC 1 cut(s) 826
AccI GTMKAC 1 cut(s) 243
AciI CCGC 7 cut(s) 14, 32, 134, 275, 368, 507, 830
AclI AACGTT 1 cut(s) 582
AclWI GGATC 1 cut(s) 85
AcoI YGGCCR 1 cut(s) 964
AcsI RAATTY 2 cut(s) 587, 727
AcuI CTGAAG 4 cut(s) 393, 657, 815, 957
AcvI CACGTG 1 cut(s) 556
AfaI GTAC 3 cut(s) 41, 177, 674
AfiI CCNNNNNNNGG 4 cut(s) 363, 410, 639, 640
AgsI TTSAA 3 cut(s) 271, 530, 587
AjnI CCWGG 1 cut(s) 303
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AleI CACNNNNGTG 1 cut(s) 554
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
AluBI AGCT 8 cut(s) 422, 443, 457, 541, 600, 647, 895, 944
AluI AGCT 8 cut(s) 422, 443, 457, 541, 600, 647, 895, 944
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 2 cut(s) 419, 437
Alw44I GTGCAC 1 cut(s) 573
AlwI GGATC 1 cut(s) 85
AoxI GGCC 4 cut(s) 365, 469, 606, 964
ApaLI GTGCAC 1 cut(s) 573
ApeKI GCWGC 5 cut(s) 5, 383, 443, 497, 644
ApoI RAATTY 2 cut(s) 587, 727
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 4 cut(s) 59, 470, 606, 711
AsuHPI GGTGA 5 cut(s) 292, 416, 427, 796, 892
AvaII GGWCC 2 cut(s) 59, 711
AxyI CCTNAGG 1 cut(s) 502
BaeGI GKGCMC 1 cut(s) 577
BalI TGGCCA 1 cut(s) 966
BanI GGYRCC 1 cut(s) 826
BbrPI CACGTG 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 5 cut(s) 17, 395, 430, 484, 656
BccI CCATC 2 cut(s) 455, 956
BciT130I CCWGG 1 cut(s) 305
BciVI GTATCC 1 cut(s) 930
BcoDI GTCTC 2 cut(s) 419, 437
BfaI CTAG 2 cut(s) 342, 363
BfmI CTRYAG 1 cut(s) 444
BfuI GTATCC 1 cut(s) 930
BglI GCCNNNNNGGC 1 cut(s) 14
BisI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 444, 498, 645, 830
BlsI GCNGC 9 cut(s) 7, 16, 136, 369, 385, 445, 499, 646, 831
Bme1390I CCNGG 1 cut(s) 305
Bme18I GGWCC 2 cut(s) 59, 711
BmgT120I GGNCC 4 cut(s) 59, 470, 606, 711
BmiI GGNNCC 4 cut(s) 87, 607, 828, 993
BmrFI CCNGG 1 cut(s) 305
BmrI ACTGGG 2 cut(s) 759, 984
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 2 cut(s) 759, 984
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 601
BpuEI CTTGAG 1 cut(s) 881
BsaAI YACGTR 1 cut(s) 556
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 4 cut(s) 9, 303, 707, 846
BsaWI WCCGGW 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 4 cut(s) 363, 410, 639, 640
Bse1I ACTGG 6 cut(s) 427, 472, 703, 765, 828, 979
Bse21I CCTNAGG 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 776
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 4 cut(s) 9, 303, 707, 846
BseLI CCNNNNNNNGG 4 cut(s) 363, 410, 639, 640
BseMI GCAATG 1 cut(s) 776
BseMII CTCAG 3 cut(s) 318, 516, 917
BseNI ACTGG 6 cut(s) 427, 472, 703, 765, 828, 979
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 5 cut(s) 17, 395, 430, 484, 656
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 4 cut(s) 367, 471, 608, 966
BshNI GGYRCC 1 cut(s) 826
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 1 cut(s) 66
BslI CCNNNNNNNGG 4 cut(s) 363, 410, 639, 640
BsmAI GTCTC 2 cut(s) 419, 437
BsmI GAATGC 2 cut(s) 702, 847
BsnI GGCC 4 cut(s) 367, 471, 608, 966
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 2 cut(s) 90, 981
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 7 cut(s) 14, 32, 134, 275, 368, 507, 830
BspANI GGCC 4 cut(s) 367, 471, 608, 966
BspCNI CTCAG 3 cut(s) 319, 515, 918
BspLI GGNNCC 4 cut(s) 87, 607, 828, 993
BspMAI CTGCAG 1 cut(s) 448
BspPI GGATC 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 826
BspTNI GGTCTC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 776
BsrI ACTGG 6 cut(s) 427, 472, 703, 765, 828, 979
BssECI CCNNGG 4 cut(s) 9, 303, 707, 846
BssMI GATC 2 cut(s) 90, 981
BssT1I CCWWGG 3 cut(s) 9, 707, 846
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 3 cut(s) 358, 692, 904
BstAPI GCANNNNNTGC 1 cut(s) 826
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 3 cut(s) 30, 277, 577
BstDEI CTNAG 5 cut(s) 327, 502, 601, 926, 951
BstDSI CCRYGG 1 cut(s) 9
BstEII GGTNACC 1 cut(s) 399
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 2 cut(s) 93, 984
BstMAI GTCTC 2 cut(s) 419, 437
BstMBI GATC 2 cut(s) 90, 981
BstMWI GCNNNNNNNGC 7 cut(s) 11, 14, 180, 332, 392, 826, 941
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 80
BstPI GGTNACC 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 303
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 577
BstV1I GCAGC 5 cut(s) 17, 395, 430, 484, 656
BstXI CCANNNNNNTGG 3 cut(s) 156, 311, 974
Bsu36I CCTNAGG 1 cut(s) 502
BsuI GTATCC 1 cut(s) 930
BsuRI GGCC 4 cut(s) 367, 471, 608, 966
BtgI CCRYGG 1 cut(s) 9
BtsIMutI CAGTG 4 cut(s) 434, 465, 688, 909
Cac8I GCNNGC 3 cut(s) 30, 277, 577
CfoI GCGC 1 cut(s) 183
Cfr13I GGNCC 4 cut(s) 59, 470, 606, 711
Csp6I GTAC 3 cut(s) 40, 176, 673
CviAII CATG 3 cut(s) 10, 77, 968
CviQI GTAC 3 cut(s) 40, 176, 673
DdeI CTNAG 5 cut(s) 327, 502, 601, 926, 951
DpnI GATC 2 cut(s) 92, 983
DpnII GATC 2 cut(s) 90, 981
DrdI GACNNNNNNGTC 1 cut(s) 66
DseDI GACNNNNNNGTC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 964
Eco130I CCWWGG 3 cut(s) 9, 707, 846
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 59, 711
Eco57I CTGAAG 4 cut(s) 393, 657, 815, 957
Eco72I CACGTG 1 cut(s) 556
Eco81I CCTNAGG 1 cut(s) 502
Eco91I GGTNACC 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 59
EcoO65I GGTNACC 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 3 cut(s) 9, 707, 846
ErhI CCWWGG 3 cut(s) 9, 707, 846
FaeI CATG 3 cut(s) 13, 80, 971
FatI CATG 3 cut(s) 9, 76, 967
FauI CCCGC 1 cut(s) 268
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 444, 498, 645, 830
Fsp4HI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 444, 498, 645, 830
FspBI CTAG 2 cut(s) 342, 363
GlaI GCGC 1 cut(s) 182
GluI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 444, 498, 645, 830
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 4 cut(s) 367, 471, 608, 966
HapII CCGG 1 cut(s) 66
HhaI GCGC 1 cut(s) 183
Hin1II CATG 3 cut(s) 13, 80, 971
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 3 cut(s) 455, 539, 942
HinfI GANTC 4 cut(s) 450, 733, 856, 911
HpaII CCGG 1 cut(s) 66
HphI GGTGA 5 cut(s) 292, 416, 427, 796, 892
Hpy166II GTNNAC 3 cut(s) 244, 575, 714
Hpy188I TCNGA 8 cut(s) 90, 102, 168, 264, 651, 669, 795, 927
Hpy188III TCNNGA 5 cut(s) 595, 620, 838, 860, 898
Hpy8I GTNNAC 3 cut(s) 244, 575, 714
Hpy99I CGWCG 2 cut(s) 47, 248
HpyAV CCTTC 8 cut(s) 72, 383, 632, 669, 739, 817, 877, 881
HpyCH4III ACNGT 3 cut(s) 358, 692, 904
HpyCH4IV ACGT 3 cut(s) 246, 555, 582
HpyCH4V TGCA 7 cut(s) 335, 395, 446, 524, 575, 695, 776
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 14, 180, 332, 392, 826, 941
HpyF3I CTNAG 5 cut(s) 327, 502, 601, 926, 951
HpySE526I ACGT 3 cut(s) 246, 555, 582
Hsp92II CATG 3 cut(s) 13, 80, 971
HspAI GCGC 1 cut(s) 181
Kzo9I GATC 2 cut(s) 90, 981
LmnI GCTCC 1 cut(s) 991
Lsp1109I GCAGC 5 cut(s) 17, 395, 430, 484, 656
LweI GCATC 1 cut(s) 667
MaeI CTAG 2 cut(s) 342, 363
MaeII ACGT 3 cut(s) 246, 555, 582
MaeIII GTNAC 5 cut(s) 358, 399, 490, 515, 556
MalI GATC 2 cut(s) 92, 983
MboI GATC 2 cut(s) 90, 981
MboII GAAGA 5 cut(s) 115, 490, 808, 950, 991
MfeI CAATTG 2 cut(s) 150, 530
MhlI GDGCHC 1 cut(s) 577
MlsI TGGCCA 1 cut(s) 966
MluCI AATT 5 cut(s) 125, 150, 530, 587, 727
MluNI TGGCCA 1 cut(s) 966
MlyI GAGTC 3 cut(s) 459, 742, 905
MmeI TCCRAC 2 cut(s) 229, 647
MnlI CCTC 8 cut(s) 50, 76, 106, 229, 308, 511, 607, 933
Mox20I TGGCCA 1 cut(s) 966
MscI TGGCCA 1 cut(s) 966
MseI TTAA 1 cut(s) 997
MslI CAYNNNNRTG 1 cut(s) 554
Msp20I TGGCCA 1 cut(s) 966
MspA1I CMGCKG 1 cut(s) 832
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 1 cut(s) 305
MunI CAATTG 2 cut(s) 150, 530
Mva1269I GAATGC 2 cut(s) 702, 847
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 7 cut(s) 11, 14, 180, 332, 392, 826, 941
NcoI CCATGG 1 cut(s) 9
NdeII GATC 2 cut(s) 90, 981
NlaIII CATG 3 cut(s) 13, 80, 971
NlaIV GGNNCC 4 cut(s) 87, 607, 828, 993
NmuCI GTSAC 2 cut(s) 515, 556
NspI RCATGY 1 cut(s) 80
OliI CACNNNNGTG 1 cut(s) 554
PctI GAATGC 2 cut(s) 702, 847
PfeI GAWTC 1 cut(s) 856
PkrI GCNGC 9 cut(s) 7, 16, 136, 369, 385, 445, 499, 646, 831
PleI GAGTC 3 cut(s) 458, 741, 905
PmaCI CACGTG 1 cut(s) 556
PmlI CACGTG 1 cut(s) 556
PpsI GAGTC 3 cut(s) 458, 741, 905
Ppu21I YACGTR 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 59
Psp1406I AACGTT 1 cut(s) 582
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 303
PspCI CACGTG 1 cut(s) 556
PspEI GGTNACC 1 cut(s) 399
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 4 cut(s) 87, 607, 828, 993
PspPI GGNCC 4 cut(s) 59, 470, 606, 711
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 1 cut(s) 448
RsaI GTAC 3 cut(s) 41, 177, 674
RsaNI GTAC 3 cut(s) 40, 176, 673
RseI CAYNNNNRTG 1 cut(s) 554
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 997
SatI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 444, 498, 645, 830
Sau3AI GATC 2 cut(s) 90, 981
Sau96I GGNCC 4 cut(s) 59, 470, 606, 711
SchI GAGTC 3 cut(s) 459, 742, 905
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 1 cut(s) 444
SinI GGWCC 2 cut(s) 59, 711
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
Sse9I AATT 5 cut(s) 125, 150, 530, 587, 727
SsiI CCGC 7 cut(s) 14, 32, 134, 275, 368, 507, 830
SspMI CTAG 2 cut(s) 342, 363
StyD4I CCNGG 1 cut(s) 303
StyI CCWWGG 3 cut(s) 9, 707, 846
TaaI ACNGT 3 cut(s) 358, 692, 904
TaiI ACGT 3 cut(s) 249, 558, 585
TaqI TCGA 1 cut(s) 243
TasI AATT 5 cut(s) 125, 150, 530, 587, 727
TauI GCSGC 4 cut(s) 17, 137, 370, 832
TfiI GAWTC 1 cut(s) 856
Tru1I TTAA 1 cut(s) 997
Tru9I TTAA 1 cut(s) 997
TscAI CASTG 4 cut(s) 434, 472, 695, 909
TseFI GTSAC 2 cut(s) 515, 556
TseI GCWGC 5 cut(s) 5, 383, 443, 497, 644
Tsp45I GTSAC 2 cut(s) 515, 556
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 4 cut(s) 434, 472, 695, 909
VneI GTGCAC 1 cut(s) 573
VpaK11BI GGWCC 2 cut(s) 59, 711
XapI RAATTY 2 cut(s) 587, 727
XceI RCATGY 1 cut(s) 80
XcmI CCANNNNNNNNNTGG 2 cut(s) 695, 820
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 2 cut(s) 342, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.