Rroxscaffold_7G00185280

Zinc-binding dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000313
Physical Location & Seq
Reverse (-)
24693101 .. 24695558
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00185280.1

Sequence Viewer

Length: 999 bp
ATGGCAGCCATGGCAGCCAAACTTATGCATGCGGTTCAGTACGACGCTTATGGTGGAGGACCTTCTGGTTTAAAGCATGTTGAGGTTCCAATCCCCACTCCCAAAAAAGATGAGGTTTTGCTGAAATTGGAAGCGGCTAGCATAAACCCAGTTGATTGGAAAATTCAGAAAGGCGTAGCGCGCCCTATTTTGCCACGCAAATTTCCTTATATACCTGTTACTGATGTGGCCGGAGAGGTTTTGGAGGTTGGACAAGGAGTTCGGAAGTTTAAAGCAGGCGACAAAGTTGTGGCATATCTCCACATCCTTAATGGAGGTGGATTTGCTGAGTTTGCAACTGCTAGTGAGAGTTTGGCGGTTACTAGGCCGCCTGAAGTTTCAGCAGCCGAAGCTGCAGGATTACCTGTTGCTGGTCTCACAGCTCACCAGTGTCTCACCCAAGCTGCAGGAGTCAAGCTTGACGGCACTGGCACACAGAAGAACATATTGGTTACTGCTGCCTCCGGTGGTGTTGGTCAGCATGCAGTCCAACTTGCAAAGCTGGGAAACACCCACGTCACAGCTACATGTGGAGCTTGTAACATTGATTTCGTCAAGAGCTTAGGGGCCGATGAGGTTCTTGACTACAAGACCCCTGAAGGGGCAGCTCTGAAGAGCCCATCTGGTCGAAAATATGATGCTGTAATCCACTGTGCGACGGGCATTCCTTGGTGCACTTTTGAGCCGAATTTGAGTCCAAATGGGAAGGTTATAGACATTACTCCCGGTCCAAGTGCTTTGTTTACTTTCGCTCTAAAGAATCTCACCTTCTCCAAGAAGAAGCTAGTGCCCCTGCTCATGAATGCCAAGGCGGAGAATCTGGATTATCTTGTGAAGTTGGTGAAGGAAGGAAAGCTGAAGACAGTGATCGACTCAAAGTATCCTCTGAGCAAGGCTGAAGATGCTTGGGCTAAGAGTATTGATGGCCATGCTACTGGGAAGATCATTGTGGAGCCTTAA

Protein Analysis

332

Amino Acids

34.88

Weight (kDa)

8.88

Isoelectric Point (pI)

26.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 121 4e-15 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 170 - 292 3e-09 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 201 - 330 1.9e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13010
fragaria_vesca FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 181
AciI CCGC 5 cut(s) 32, 134, 356, 368, 851
AcoI YGGCCR 2 cut(s) 228, 964
AcsI RAATTY 3 cut(s) 162, 200, 727
AcuI CTGAAG 5 cut(s) 393, 657, 671, 917, 957
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 3 cut(s) 410, 639, 640
AflIII ACRYGT 1 cut(s) 566
AjiI CACGTC 1 cut(s) 556
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
Alw21I GWGCWC 1 cut(s) 716
Alw26I GTCTC 2 cut(s) 419, 437
Alw44I GTGCAC 1 cut(s) 712
AoxI GGCC 4 cut(s) 228, 365, 606, 964
ApaLI GTGCAC 1 cut(s) 712
ApeKI GCWGC 7 cut(s) 5, 14, 383, 392, 443, 497, 644
ApoI RAATTY 3 cut(s) 162, 200, 727
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AspLEI GCGC 2 cut(s) 181, 183
AspS9I GGNCC 3 cut(s) 59, 606, 767
AsuC2I CCSGG 1 cut(s) 765
AsuHPI GGTGA 4 cut(s) 416, 427, 796, 892
AsuNHI GCTAGC 1 cut(s) 137
AvaII GGWCC 2 cut(s) 59, 767
BaeGI GKGCMC 2 cut(s) 716, 831
BalI TGGCCA 1 cut(s) 966
BanII GRGCYC 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 716
BbvI GCAGC 7 cut(s) 17, 26, 379, 395, 430, 484, 656
BccI CCATC 2 cut(s) 667, 956
BceAI ACGGC 1 cut(s) 478
BciVI GTATCC 1 cut(s) 930
BcnI CCSGG 1 cut(s) 765
BcoDI GTCTC 2 cut(s) 419, 437
BfaI CTAG 4 cut(s) 138, 342, 363, 824
BfmI CTRYAG 2 cut(s) 393, 444
BfuI GTATCC 1 cut(s) 930
BisI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 393, 444, 498, 645
BlsI GCNGC 9 cut(s) 7, 16, 136, 369, 385, 394, 445, 499, 646
Bme1390I CCNGG 1 cut(s) 765
Bme18I GGWCC 2 cut(s) 59, 767
BmgBI CACGTC 1 cut(s) 556
BmgT120I GGNCC 3 cut(s) 59, 606, 767
BmiI GGNNCC 3 cut(s) 87, 607, 993
BmrFI CCNGG 1 cut(s) 765
BmrI ACTGGG 2 cut(s) 143, 984
BmsI GCATC 2 cut(s) 667, 931
BmtI GCTAGC 1 cut(s) 141
BmuI ACTGGG 2 cut(s) 143, 984
BpiI GAAGAC 1 cut(s) 905
Bpu10I CCTNAGC 1 cut(s) 601
BpuMI CCSGG 1 cut(s) 765
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 3 cut(s) 9, 707, 846
BsaWI WCCGGW 1 cut(s) 503
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 639, 640
Bse1I ACTGG 4 cut(s) 149, 427, 472, 979
BseDI CCNNGG 3 cut(s) 9, 707, 846
BseGI GGATG 1 cut(s) 303
BseLI CCNNNNNNNGG 3 cut(s) 410, 639, 640
BseMII CTCAG 2 cut(s) 318, 917
BseNI ACTGG 4 cut(s) 149, 427, 472, 979
BsePI GCGCGC 1 cut(s) 179
BseSI GKGCMC 2 cut(s) 716, 831
BseXI GCAGC 7 cut(s) 17, 26, 379, 395, 430, 484, 656
BseYI CCCAGC 1 cut(s) 541
Bsh1236I CGCG 1 cut(s) 181
BshFI GGCC 4 cut(s) 230, 367, 608, 966
BsiHKAI GWGCWC 1 cut(s) 716
BsiSI CCGG 3 cut(s) 231, 504, 765
BslI CCNNNNNNNGG 3 cut(s) 410, 639, 640
BsmAI GTCTC 2 cut(s) 419, 437
BsmI GAATGC 2 cut(s) 702, 847
BsnI GGCC 4 cut(s) 230, 367, 608, 966
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 3 cut(s) 659, 716, 831
Bsp143I GATC 2 cut(s) 906, 981
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 5 cut(s) 32, 134, 356, 368, 851
BspANI GGCC 4 cut(s) 230, 367, 608, 966
BspCNI CTCAG 2 cut(s) 319, 918
BspFNI CGCG 1 cut(s) 181
BspHI TCATGA 1 cut(s) 837
BspLI GGNNCC 3 cut(s) 87, 607, 993
BspMAI CTGCAG 2 cut(s) 397, 448
BspOI GCTAGC 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 419
BsrI ACTGG 4 cut(s) 149, 427, 472, 979
BssECI CCNNGG 3 cut(s) 9, 707, 846
BssHII GCGCGC 1 cut(s) 179
BssMI GATC 2 cut(s) 906, 981
BssT1I CCWWGG 3 cut(s) 9, 707, 846
Bst4CI ACNGT 2 cut(s) 692, 904
Bst6I CTCTTC 1 cut(s) 647
BstC8I GCNNGC 5 cut(s) 30, 139, 181, 277, 522
BstDEI CTNAG 4 cut(s) 327, 601, 926, 951
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 181
BstHHI GCGC 2 cut(s) 181, 183
BstKTI GATC 2 cut(s) 909, 984
BstMAI GTCTC 2 cut(s) 419, 437
BstMBI GATC 2 cut(s) 906, 981
BstMWI GCNNNNNNNGC 7 cut(s) 11, 14, 180, 332, 389, 392, 941
BstNSI RCATGY 4 cut(s) 32, 80, 524, 570
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 2 cut(s) 393, 444
BstSLI GKGCMC 2 cut(s) 716, 831
BstUI CGCG 1 cut(s) 181
BstV1I GCAGC 7 cut(s) 17, 26, 379, 395, 430, 484, 656
BstV2I GAAGAC 1 cut(s) 905
BstXI CCANNNNNNTGG 2 cut(s) 156, 974
BsuI GTATCC 1 cut(s) 930
BsuRI GGCC 4 cut(s) 230, 367, 608, 966
BtgI CCRYGG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 556
BtsCI GGATG 1 cut(s) 303
BtsIMutI CAGTG 4 cut(s) 434, 465, 688, 909
Cac8I GCNNGC 5 cut(s) 30, 139, 181, 277, 522
CciI TCATGA 1 cut(s) 837
CfoI GCGC 2 cut(s) 181, 183
Cfr13I GGNCC 3 cut(s) 59, 606, 767
CseI GACGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 40
CviAII CATG 7 cut(s) 10, 29, 77, 521, 567, 838, 968
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 4 cut(s) 327, 601, 926, 951
DpnI GATC 2 cut(s) 908, 983
DpnII GATC 2 cut(s) 906, 981
DraI TTTAAA 2 cut(s) 72, 271
EaeI YGGCCR 2 cut(s) 228, 964
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
EciI GGCGGA 1 cut(s) 866
Eco130I CCWWGG 3 cut(s) 9, 707, 846
Eco24I GRGCYC 1 cut(s) 659
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 59, 767
Eco57I CTGAAG 5 cut(s) 393, 657, 671, 917, 957
EcoO109I RGGNCCY 1 cut(s) 59
EcoT14I CCWWGG 3 cut(s) 9, 707, 846
EcoT22I ATGCAT 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 3 cut(s) 9, 707, 846
FaeI CATG 7 cut(s) 13, 32, 80, 524, 570, 841, 971
FatI CATG 7 cut(s) 9, 28, 76, 520, 566, 837, 967
Fnu4HI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 393, 444, 498, 645
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 393, 444, 498, 645
FspBI CTAG 4 cut(s) 138, 342, 363, 824
GlaI GCGC 2 cut(s) 180, 182
GluI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 393, 444, 498, 645
GsaI CCCAGC 1 cut(s) 545
HaeIII GGCC 4 cut(s) 230, 367, 608, 966
HapII CCGG 3 cut(s) 231, 504, 765
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 181, 183
Hin1II CATG 7 cut(s) 13, 32, 80, 524, 570, 841, 971
Hin6I GCGC 2 cut(s) 179, 181
HinP1I GCGC 2 cut(s) 179, 181
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 5 cut(s) 450, 733, 799, 856, 911
HpaII CCGG 3 cut(s) 231, 504, 765
HphI GGTGA 4 cut(s) 416, 427, 796, 892
Hpy166II GTNNAC 2 cut(s) 714, 783
Hpy188I TCNGA 4 cut(s) 168, 264, 651, 927
Hpy188III TCNNGA 4 cut(s) 595, 620, 838, 860
Hpy8I GTNNAC 2 cut(s) 714, 783
Hpy99I CGWCG 2 cut(s) 47, 700
HpyAV CCTTC 6 cut(s) 72, 632, 739, 817, 877, 881
HpyCH4III ACNGT 2 cut(s) 692, 904
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 7 cut(s) 28, 335, 395, 446, 524, 536, 714
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 14, 180, 332, 389, 392, 941
HpyF3I CTNAG 4 cut(s) 327, 601, 926, 951
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 7 cut(s) 13, 32, 80, 524, 570, 841, 971
HspAI GCGC 2 cut(s) 179, 181
Kzo9I GATC 2 cut(s) 906, 981
LguI GCTCTTC 1 cut(s) 647
LmnI GCTCC 2 cut(s) 572, 991
Lsp1109I GCAGC 7 cut(s) 17, 26, 379, 395, 430, 484, 656
LweI GCATC 2 cut(s) 667, 931
MaeI CTAG 4 cut(s) 138, 342, 363, 824
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 5 cut(s) 217, 358, 490, 556, 578
MalI GATC 2 cut(s) 908, 983
MboI GATC 2 cut(s) 906, 981
MboII GAAGA 6 cut(s) 490, 664, 829, 910, 950, 991
MhlI GDGCHC 3 cut(s) 659, 716, 831
MlsI TGGCCA 1 cut(s) 966
MluCI AATT 4 cut(s) 125, 162, 200, 727
MluNI TGGCCA 1 cut(s) 966
MlyI GAGTC 3 cut(s) 459, 742, 905
MmeI TCCRAC 2 cut(s) 229, 553
MnlI CCTC 9 cut(s) 50, 76, 106, 229, 238, 308, 511, 607, 933
Mox20I TGGCCA 1 cut(s) 966
Mph1103I ATGCAT 1 cut(s) 30
MscI TGGCCA 1 cut(s) 966
MseI TTAA 4 cut(s) 71, 270, 309, 997
Msp20I TGGCCA 1 cut(s) 966
MspI CCGG 3 cut(s) 231, 504, 765
MspR9I CCNGG 1 cut(s) 765
Mva1269I GAATGC 2 cut(s) 702, 847
MvnI CGCG 1 cut(s) 181
MwoI GCNNNNNNNGC 7 cut(s) 11, 14, 180, 332, 389, 392, 941
NciI CCSGG 1 cut(s) 765
NcoI CCATGG 1 cut(s) 9
NdeII GATC 2 cut(s) 906, 981
NheI GCTAGC 1 cut(s) 137
NlaIII CATG 7 cut(s) 13, 32, 80, 524, 570, 841, 971
NlaIV GGNNCC 3 cut(s) 87, 607, 993
NmuCI GTSAC 1 cut(s) 556
NsiI ATGCAT 1 cut(s) 30
NspI RCATGY 4 cut(s) 32, 80, 524, 570
PaeI GCATGC 2 cut(s) 32, 524
PagI TCATGA 1 cut(s) 837
PauI GCGCGC 1 cut(s) 179
PciI ACATGT 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 647
PctI GAATGC 2 cut(s) 702, 847
PfeI GAWTC 2 cut(s) 799, 856
PkrI GCNGC 9 cut(s) 7, 16, 136, 369, 385, 394, 445, 499, 646
PleI GAGTC 3 cut(s) 458, 741, 905
PpsI GAGTC 3 cut(s) 458, 741, 905
PpuMI RGGWCCY 1 cut(s) 59
PscI ACATGT 1 cut(s) 566
Psp5II RGGWCCY 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 541
PspN4I GGNNCC 3 cut(s) 87, 607, 993
PspPI GGNCC 3 cut(s) 59, 606, 767
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 2 cut(s) 397, 448
PteI GCGCGC 1 cut(s) 179
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SapI GCTCTTC 1 cut(s) 647
SaqAI TTAA 4 cut(s) 71, 270, 309, 997
SatI GCNGC 9 cut(s) 6, 15, 135, 368, 384, 393, 444, 498, 645
Sau3AI GATC 2 cut(s) 906, 981
Sau96I GGNCC 3 cut(s) 59, 606, 767
SchI GAGTC 3 cut(s) 459, 742, 905
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 3 cut(s) 659, 716, 831
SfaNI GCATC 2 cut(s) 667, 931
SfcI CTRYAG 2 cut(s) 393, 444
SinI GGWCC 2 cut(s) 59, 767
SphI GCATGC 2 cut(s) 32, 524
Sse9I AATT 4 cut(s) 125, 162, 200, 727
SsiI CCGC 5 cut(s) 32, 134, 356, 368, 851
SspMI CTAG 4 cut(s) 138, 342, 363, 824
StyD4I CCNGG 1 cut(s) 763
StyI CCWWGG 3 cut(s) 9, 707, 846
TaaI ACNGT 2 cut(s) 692, 904
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 667, 909
TasI AATT 4 cut(s) 125, 162, 200, 727
TauI GCSGC 2 cut(s) 137, 370
TfiI GAWTC 2 cut(s) 799, 856
Tru1I TTAA 4 cut(s) 71, 270, 309, 997
Tru9I TTAA 4 cut(s) 71, 270, 309, 997
TscAI CASTG 4 cut(s) 434, 472, 695, 909
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 7 cut(s) 5, 14, 383, 392, 443, 497, 644
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 1 cut(s) 854
TspRI CASTG 4 cut(s) 434, 472, 695, 909
VneI GTGCAC 1 cut(s) 712
VpaK11BI GGWCC 2 cut(s) 59, 767
XapI RAATTY 3 cut(s) 162, 200, 727
XceI RCATGY 4 cut(s) 32, 80, 524, 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XspI CTAG 4 cut(s) 138, 342, 363, 824
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.