Rh6DG264600

Zinc-binding dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000313
Physical Location & Seq
Forward (+)
45654664 .. 45657096
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG264600.1

Sequence Viewer

Length: 756 bp
ATGGCGGCCAAACTTATGCATGCGGTTCAGTACGACGCTTATGGTGGAGGACCTTCTGGTTTAAAGAATGGAGGTGGATTTGCTGAGTTTGCAACTGCTAGTGAGAATTTGACGGTTACTAGGCCGCCTGAAGTTTCAGCAGCCGAAGCTGCAGGATTACCTGTTGCTGGTCTCACAGCTCACCAGTGTCTCACCCAAGCTGCAGGAGTCAAGCTTGACGGCACTGGCCCACAGAAGAACATACTGGTTACTGCTGCCTCCGGTGGTGTGGGTCAGTATGCAGTCCAACTTGCAAAGCTGGGAAACACCCACGTCACAGCTACATGTGGAGCTCGTAACATTGATTTCGTCAAGAGCTTAGGGGCCGATGAGGTTCTTGACTACAAGACCCCTGAAGGGGCAGCTCTGAAGAGCCCATCTGGTCGCAAATATGATGCTGTAATCCACTGTGCGACGGGCATTCCTTGGTGCACTTTTGAGCCGAATTTGAGTCCAAATGGGAAGGTTATAGACATTACTCCTGGTCCAAGTGCTTTGTTTACTTTCGCTCTAAAGAATCTCACCTTCTCCAAGAAGAAGCTAGTGCCGCTGCTCATGAATGCCAAGGCGGAGAATCTGGATTATCTTGTGAAGTTGGTGAAGGAAGGAAAGCTGAAGACAGTGATCGACTCAAAGTATCCTCTGAGCAAGGCTGAAGATGCTTGGGCTAAGAGTATTGATGGCCATGCTGCTGGGAAGATCATTGTGGAGCCTTAA

Protein Analysis

251

Amino Acids

26.09

Weight (kDa)

8.75

Isoelectric Point (pI)

28.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 89 - 211 2.4e-10 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 120 - 249 2.6e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13010
fragaria_vesca FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 5, 23, 125, 587, 608
AcoI YGGCCR 2 cut(s) 6, 721
AcsI RAATTY 2 cut(s) 106, 484
AcuI CTGAAG 5 cut(s) 150, 414, 428, 674, 714
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 3 cut(s) 167, 396, 397
AflIII ACRYGT 1 cut(s) 323
AjiI CACGTC 1 cut(s) 313
AjnI CCWGG 1 cut(s) 520
Alw21I GWGCWC 2 cut(s) 334, 473
Alw26I GTCTC 2 cut(s) 176, 194
Alw44I GTGCAC 1 cut(s) 469
AoxI GGCC 5 cut(s) 6, 122, 226, 363, 721
ApaLI GTGCAC 1 cut(s) 469
ApeKI GCWGC 7 cut(s) 140, 149, 200, 254, 401, 589, 728
ApoI RAATTY 2 cut(s) 106, 484
AspS9I GGNCC 4 cut(s) 50, 227, 363, 524
AsuHPI GGTGA 4 cut(s) 173, 184, 553, 649
AvaII GGWCC 2 cut(s) 50, 524
BaeGI GKGCMC 1 cut(s) 473
BalI TGGCCA 1 cut(s) 723
BanII GRGCYC 2 cut(s) 334, 416
BbsI GAAGAC 1 cut(s) 662
Bbv12I GWGCWC 2 cut(s) 334, 473
BbvI GCAGC 7 cut(s) 136, 152, 187, 241, 413, 576, 715
BccI CCATC 2 cut(s) 424, 713
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 522
BciVI GTATCC 1 cut(s) 687
BcoDI GTCTC 2 cut(s) 176, 194
BfaI CTAG 3 cut(s) 99, 120, 581
BfmI CTRYAG 2 cut(s) 150, 201
BfuI GTATCC 1 cut(s) 687
Bme1390I CCNGG 1 cut(s) 522
Bme18I GGWCC 2 cut(s) 50, 524
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 4 cut(s) 50, 227, 363, 524
BmiI GGNNCC 2 cut(s) 364, 750
BmrFI CCNGG 1 cut(s) 522
BmsI GCATC 2 cut(s) 424, 688
BpiI GAAGAC 1 cut(s) 662
Bpu10I CCTNAGC 1 cut(s) 358
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 464, 603
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 396, 397
Bse1I ACTGG 3 cut(s) 184, 229, 249
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 464, 603
BseLI CCNNNNNNNGG 3 cut(s) 167, 396, 397
BseMII CTCAG 2 cut(s) 75, 674
BseNI ACTGG 3 cut(s) 184, 229, 249
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 7 cut(s) 136, 152, 187, 241, 413, 576, 715
BseYI CCCAGC 2 cut(s) 298, 731
BshFI GGCC 5 cut(s) 8, 124, 228, 365, 723
BsiHKAI GWGCWC 2 cut(s) 334, 473
BsiSI CCGG 1 cut(s) 261
BslI CCNNNNNNNGG 3 cut(s) 167, 396, 397
BsmAI GTCTC 2 cut(s) 176, 194
BsmI GAATGC 2 cut(s) 459, 604
BsnI GGCC 5 cut(s) 8, 124, 228, 365, 723
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 3 cut(s) 334, 416, 473
Bsp143I GATC 2 cut(s) 663, 738
BspACI CCGC 5 cut(s) 5, 23, 125, 587, 608
BspANI GGCC 5 cut(s) 8, 124, 228, 365, 723
BspCNI CTCAG 2 cut(s) 76, 675
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 2 cut(s) 364, 750
BspMAI CTGCAG 2 cut(s) 154, 205
BspQI GCTCTTC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 3 cut(s) 184, 229, 249
BssECI CCNNGG 2 cut(s) 464, 603
BssMI GATC 2 cut(s) 663, 738
BssT1I CCWWGG 2 cut(s) 464, 603
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 3 cut(s) 115, 449, 661
Bst6I CTCTTC 1 cut(s) 404
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 4 cut(s) 84, 358, 683, 708
BstKTI GATC 2 cut(s) 666, 741
BstMAI GTCTC 2 cut(s) 176, 194
BstMBI GATC 2 cut(s) 663, 738
BstMWI GCNNNNNNNGC 5 cut(s) 89, 146, 149, 586, 698
BstNI CCWGG 1 cut(s) 522
BstNSI RCATGY 2 cut(s) 23, 327
BstSCI CCNGG 1 cut(s) 520
BstSFI CTRYAG 2 cut(s) 150, 201
BstSLI GKGCMC 1 cut(s) 473
BstV1I GCAGC 7 cut(s) 136, 152, 187, 241, 413, 576, 715
BstV2I GAAGAC 1 cut(s) 662
BstXI CCANNNNNNTGG 1 cut(s) 731
BsuI GTATCC 1 cut(s) 687
BsuRI GGCC 5 cut(s) 8, 124, 228, 365, 723
BtrI CACGTC 1 cut(s) 313
BtsIMutI CAGTG 4 cut(s) 191, 222, 445, 666
Cac8I GCNNGC 1 cut(s) 21
CciI TCATGA 1 cut(s) 594
Cfr13I GGNCC 4 cut(s) 50, 227, 363, 524
CseI GACGC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 31
CviAII CATG 4 cut(s) 20, 324, 595, 725
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 4 cut(s) 84, 358, 683, 708
DpnI GATC 2 cut(s) 665, 740
DpnII GATC 2 cut(s) 663, 738
DraI TTTAAA 1 cut(s) 63
EaeI YGGCCR 2 cut(s) 6, 721
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
EciI GGCGGA 1 cut(s) 623
Ecl136II GAGCTC 1 cut(s) 332
Eco130I CCWWGG 2 cut(s) 464, 603
Eco24I GRGCYC 2 cut(s) 334, 416
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 50, 524
Eco53kI GAGCTC 1 cut(s) 332
Eco57I CTGAAG 5 cut(s) 150, 414, 428, 674, 714
EcoICRI GAGCTC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 520
EcoT14I CCWWGG 2 cut(s) 464, 603
EcoT22I ATGCAT 1 cut(s) 21
EcoT38I GRGCYC 2 cut(s) 334, 416
ErhI CCWWGG 2 cut(s) 464, 603
FaeI CATG 4 cut(s) 23, 327, 598, 728
FatI CATG 4 cut(s) 19, 323, 594, 724
FriOI GRGCYC 2 cut(s) 334, 416
FspBI CTAG 3 cut(s) 99, 120, 581
GsaI CCCAGC 2 cut(s) 302, 735
HaeIII GGCC 5 cut(s) 8, 124, 228, 365, 723
HapII CCGG 1 cut(s) 261
HgaI GACGC 1 cut(s) 44
Hin1II CATG 4 cut(s) 23, 327, 598, 728
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 5 cut(s) 207, 490, 556, 613, 668
HpaII CCGG 1 cut(s) 261
HphI GGTGA 4 cut(s) 173, 184, 553, 649
Hpy166II GTNNAC 2 cut(s) 471, 540
Hpy188I TCNGA 2 cut(s) 408, 684
Hpy188III TCNNGA 4 cut(s) 352, 377, 595, 617
Hpy8I GTNNAC 2 cut(s) 471, 540
Hpy99I CGWCG 2 cut(s) 38, 457
HpyAV CCTTC 6 cut(s) 63, 389, 496, 574, 634, 638
HpyCH4III ACNGT 3 cut(s) 115, 449, 661
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 7 cut(s) 19, 92, 152, 203, 281, 293, 471
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 146, 149, 586, 698
HpyF3I CTNAG 4 cut(s) 84, 358, 683, 708
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 4 cut(s) 23, 327, 598, 728
Kzo9I GATC 2 cut(s) 663, 738
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 2 cut(s) 329, 748
Lsp1109I GCAGC 7 cut(s) 136, 152, 187, 241, 413, 576, 715
LweI GCATC 2 cut(s) 424, 688
MaeI CTAG 3 cut(s) 99, 120, 581
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 4 cut(s) 115, 247, 313, 335
MalI GATC 2 cut(s) 665, 740
MboI GATC 2 cut(s) 663, 738
MboII GAAGA 6 cut(s) 247, 421, 586, 667, 707, 748
MhlI GDGCHC 3 cut(s) 334, 416, 473
MlsI TGGCCA 1 cut(s) 723
MluCI AATT 2 cut(s) 106, 484
MluNI TGGCCA 1 cut(s) 723
MlyI GAGTC 3 cut(s) 216, 499, 662
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 5 cut(s) 41, 65, 268, 364, 690
Mox20I TGGCCA 1 cut(s) 723
Mph1103I ATGCAT 1 cut(s) 21
MscI TGGCCA 1 cut(s) 723
MseI TTAA 2 cut(s) 62, 754
Msp20I TGGCCA 1 cut(s) 723
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 1 cut(s) 522
Mva1269I GAATGC 2 cut(s) 459, 604
MvaI CCWGG 1 cut(s) 522
MwoI GCNNNNNNNGC 5 cut(s) 89, 146, 149, 586, 698
NdeII GATC 2 cut(s) 663, 738
NlaIII CATG 4 cut(s) 23, 327, 598, 728
NlaIV GGNNCC 2 cut(s) 364, 750
NmuCI GTSAC 1 cut(s) 313
NsiI ATGCAT 1 cut(s) 21
NspI RCATGY 2 cut(s) 23, 327
PaeI GCATGC 1 cut(s) 23
PagI TCATGA 1 cut(s) 594
PciI ACATGT 1 cut(s) 323
PciSI GCTCTTC 1 cut(s) 404
PctI GAATGC 2 cut(s) 459, 604
PfeI GAWTC 2 cut(s) 556, 613
PleI GAGTC 3 cut(s) 215, 498, 662
PpsI GAGTC 3 cut(s) 215, 498, 662
PpuMI RGGWCCY 1 cut(s) 50
PscI ACATGT 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 520
PspFI CCCAGC 2 cut(s) 298, 731
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 364, 750
PspPI GGNCC 4 cut(s) 50, 227, 363, 524
PspPPI RGGWCCY 1 cut(s) 50
PstI CTGCAG 2 cut(s) 154, 205
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SacI GAGCTC 1 cut(s) 334
SapI GCTCTTC 1 cut(s) 404
SaqAI TTAA 2 cut(s) 62, 754
Sau3AI GATC 2 cut(s) 663, 738
Sau96I GGNCC 4 cut(s) 50, 227, 363, 524
SchI GAGTC 3 cut(s) 216, 499, 662
ScrFI CCNGG 1 cut(s) 522
SduI GDGCHC 3 cut(s) 334, 416, 473
SfaNI GCATC 2 cut(s) 424, 688
SfcI CTRYAG 2 cut(s) 150, 201
SinI GGWCC 2 cut(s) 50, 524
SphI GCATGC 1 cut(s) 23
Sse9I AATT 2 cut(s) 106, 484
SsiI CCGC 5 cut(s) 5, 23, 125, 587, 608
SspMI CTAG 3 cut(s) 99, 120, 581
SstI GAGCTC 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 2 cut(s) 464, 603
TaaI ACNGT 3 cut(s) 115, 449, 661
TaiI ACGT 1 cut(s) 315
TaqI TCGA 1 cut(s) 666
TasI AATT 2 cut(s) 106, 484
TauI GCSGC 3 cut(s) 8, 127, 589
TfiI GAWTC 2 cut(s) 556, 613
Tru1I TTAA 2 cut(s) 62, 754
Tru9I TTAA 2 cut(s) 62, 754
TscAI CASTG 4 cut(s) 191, 229, 452, 666
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 7 cut(s) 140, 149, 200, 254, 401, 589, 728
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 1 cut(s) 611
TspRI CASTG 4 cut(s) 191, 229, 452, 666
VneI GTGCAC 1 cut(s) 469
VpaK11BI GGWCC 2 cut(s) 50, 524
XapI RAATTY 2 cut(s) 106, 484
XceI RCATGY 2 cut(s) 23, 327
XspI CTAG 3 cut(s) 99, 120, 581
Zsp2I ATGCAT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.