Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000313

Physical Location & Seq

Genomic Coordinates Reverse (-)

29863248 .. 29866278

Gene Structure 3031 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1168700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGGCGTCAAAGCTTATGCATGCGGTACAGTACGACAGCTATGGCGGAGGCGCTTCTGCTTTAAAGCATGTGGAGGCTCCGGTCCCCTCTCCCAAGAAGGGGGAGGTTTTGTTGAAACTGGAAGCAGCTGCTCTAAACCCAGTTGATTGGAAAATTCAGAAGGGCATGCTTCGGCCTTTTTTCCCCCGAAAATTTCCTCACATACCTGTTACTGATGTGGCTGGAGAGGTTGTAGAGGTTGGACAAGGAGTGCAAAAGTTCAAACCGGGGGACAAAGTTGTGACATTTCTGAGCCATGCTAATGGAGGTGGACTGGCTGAGTACACTAAAGCTAGTGAGAACTTGATAGTGTCCAGGCCACCTGAAGTCTCAGCAGCTGAAGGTGCAGGCTTACCTGTTGCTGGCCTCACAGCTCACCAGTGTCTCACGGAATCTGCTGGGATCAAGCTGGACGGAAGCGGCCAGCAAAAGAACATATTGATTACTGCTGCCTCTGGGGGCGTGGGTCAGTATGCAGTCCAACTAGCAAAGCTCGGTAACACGCATGTTACAGCCACGTGTGGAGCTCGTAACATTGAATTTGTCCAGAGCTTAGGGGCAGATGAGGTTCTTGACTACAAGACCCCAGAAGGAGCAGCTCTGACTAGCCCATCTGGTCGGAAATATGATGTTGTGGTCCACTGCGCAACTGGCATCCCCTGGTCAACTTTTGAGCCTAATTTGAGTACCAACGGGAAGGTAATAGACATCACTCCCAGTCCAAGTGCCTTTGCTACTTTTGCACTGAAGAAACTCACATTCTCGAAGAAGCAGCTGGTGCCACTGTTTCTGAATGTCAAGGCTGAGAACTTGGAGTATCTTGTTAAGTTGGTGAAGGAAGGAAAGCTCAAGACGGTAATCGACTCGACGTATTCTCTGAGCAAGGCTGAAGATGCTTGGGCTAGGAGTATGGATGGACACGCGACTGGAAAGATTATTGTCGAGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N_2

Protein Analysis

330

Amino Acids

34.82

Weight (kDa)

8.75

Isoelectric Point (pI)

33.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	33 - 119	1.5e-16	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	167 - 279	3.2e-09	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	198 - 327	2.1e-23	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13010
fragaria_vesca	FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica	MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica	Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis	pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis	RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata	RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora	Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa	Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha	Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana	Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	685
AccB1I	GGYRCC	1 cut(s)	817
AccII	CGCG	1 cut(s)	962
AciI	CCGC	3 cut(s)	23, 45, 459
AclWI	GGATC	1 cut(s)	449
AcoI	YGGCCR	1 cut(s)	460
AcsI	RAATTY	3 cut(s)	153, 191, 578
AcuI	CTGAAG	4 cut(s)	384, 399, 806, 948
AcvI	CACGTG	1 cut(s)	558
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	4 cut(s)	27, 32, 323, 727
AfiI	CCNNNNNNNGG	3 cut(s)	98, 99, 401
AflIII	ACRYGT	1 cut(s)	557
AgsI	TTSAA	3 cut(s)	115, 262, 578
AjnI	CCWGG	2 cut(s)	353, 698
Alw21I	GWGCWC	1 cut(s)	568
Alw26I	GTCTC	2 cut(s)	373, 428
AlwI	GGATC	1 cut(s)	449
AlwNI	CAGNNNCTG	1 cut(s)	377
AoxI	GGCC	4 cut(s)	173, 356, 403, 460
ApeKI	GCWGC	6 cut(s)	125, 128, 374, 488, 635, 811
ApoI	RAATTY	3 cut(s)	153, 191, 578
AspLEI	GCGC	2 cut(s)	53, 686
AspS9I	GGNCC	2 cut(s)	82, 676
AsuC2I	CCSGG	1 cut(s)	267
AsuHPI	GGTGA	2 cut(s)	407, 883
AvaII	GGWCC	2 cut(s)	82, 676
BanI	GGYRCC	1 cut(s)	817
BanII	GRGCYC	1 cut(s)	568
BbrPI	CACGTG	1 cut(s)	558
Bbv12I	GWGCWC	1 cut(s)	568
BbvI	GCAGC	6 cut(s)	115, 137, 386, 475, 647, 823
BccI	CCATC	2 cut(s)	658, 947
BciT130I	CCWGG	2 cut(s)	355, 700
BcnI	CCSGG	1 cut(s)	267
BcoDI	GTCTC	2 cut(s)	373, 428
BfaI	CTAG	4 cut(s)	333, 524, 645, 942
BfoI	RGCGCY	1 cut(s)	54
BisI	GCNGC	7 cut(s)	126, 129, 375, 460, 489, 636, 812
BlsI	GCNGC	7 cut(s)	127, 130, 376, 461, 490, 637, 813
Bme1390I	CCNGG	3 cut(s)	267, 355, 700
Bme18I	GGWCC	2 cut(s)	82, 676
BmgT120I	GGNCC	2 cut(s)	82, 676
BmiI	GGNNCC	3 cut(s)	78, 84, 819
BmrFI	CCNGG	3 cut(s)	267, 355, 700
BmrI	ACTGGG	2 cut(s)	134, 750
BmsI	GCATC	2 cut(s)	702, 922
BmuI	ACTGGG	2 cut(s)	134, 750
BpmI	CTGGAG	1 cut(s)	243
Bpu10I	CCTNAGC	1 cut(s)	592
BpuEI	CTTGAG	1 cut(s)	872
BpuMI	CCSGG	1 cut(s)	267
BsaAI	YACGTR	1 cut(s)	558
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	2 cut(s)	266, 698
BsaWI	WCCGGW	1 cut(s)	79
BsaXI	ACNNNNNCTCC	4 cut(s)	95, 125, 216, 246
Bsc4I	CCNNNNNNNGG	3 cut(s)	98, 99, 401
Bse1I	ACTGG	7 cut(s)	123, 140, 318, 418, 694, 756, 970
BseBI	CCWGG	2 cut(s)	355, 700
BseDI	CCNNGG	2 cut(s)	266, 698
BseGI	GGATG	2 cut(s)	693, 958
BseLI	CCNNNNNNNGG	3 cut(s)	98, 99, 401
BseMII	CTCAG	5 cut(s)	281, 309, 384, 834, 908
BseNI	ACTGG	7 cut(s)	123, 140, 318, 418, 694, 756, 970
BseXI	GCAGC	6 cut(s)	115, 137, 386, 475, 647, 823
BseYI	CCCAGC	1 cut(s)	437
BsgI	GTGCAG	1 cut(s)	405
Bsh1236I	CGCG	1 cut(s)	962
BshFI	GGCC	4 cut(s)	175, 358, 405, 462
BshNI	GGYRCC	1 cut(s)	817
BsiHKAI	GWGCWC	1 cut(s)	568
BsiSI	CCGG	2 cut(s)	80, 266
BslFI	GGGAC	2 cut(s)	68, 284
BslI	CCNNNNNNNGG	3 cut(s)	98, 99, 401
BsmAI	GTCTC	2 cut(s)	373, 428
BsmFI	GGGAC	2 cut(s)	68, 284
BsnI	GGCC	4 cut(s)	175, 358, 405, 462
Bsp1286I	GDGCHC	1 cut(s)	568
Bsp143I	GATC	1 cut(s)	441
BspACI	CCGC	3 cut(s)	23, 45, 459
BspANI	GGCC	4 cut(s)	175, 358, 405, 462
BspCNI	CTCAG	5 cut(s)	282, 310, 383, 835, 909
BspFNI	CGCG	1 cut(s)	962
BspLI	GGNNCC	3 cut(s)	78, 84, 819
BspPI	GGATC	1 cut(s)	449
BspT107I	GGYRCC	1 cut(s)	817
BsrI	ACTGG	7 cut(s)	123, 140, 318, 418, 694, 756, 970
BssECI	CCNNGG	2 cut(s)	266, 698
BssMI	GATC	1 cut(s)	441
BssNI	GRCGYC	1 cut(s)	5
Bst2UI	CCWGG	2 cut(s)	355, 700
Bst4CI	ACNGT	3 cut(s)	30, 825, 895
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	1 cut(s)	817
BstBAI	YACGTR	1 cut(s)	558
BstC8I	GCNNGC	5 cut(s)	21, 167, 388, 403, 464
BstDEI	CTNAG	6 cut(s)	290, 318, 370, 592, 843, 917
BstF5I	GGATG	2 cut(s)	693, 958
BstFNI	CGCG	1 cut(s)	962
BstH2I	RGCGCY	1 cut(s)	54
BstHHI	GCGC	2 cut(s)	53, 686
BstKTI	GATC	1 cut(s)	444
BstMAI	GTCTC	2 cut(s)	373, 428
BstMBI	GATC	1 cut(s)	441
BstMWI	GCNNNNNNNGC	5 cut(s)	383, 690, 779, 817, 932
BstNI	CCWGG	2 cut(s)	355, 700
BstNSI	RCATGY	4 cut(s)	23, 71, 169, 548
BstSCI	CCNGG	3 cut(s)	265, 353, 698
BstUI	CGCG	1 cut(s)	962
BstV1I	GCAGC	6 cut(s)	115, 137, 386, 475, 647, 823
BstXI	CCANNNNNNTGG	2 cut(s)	147, 302
BsuRI	GGCC	4 cut(s)	175, 358, 405, 462
BtsCI	GGATG	2 cut(s)	693, 958
BtsI	GCAGTG	1 cut(s)	679
BtsIMutI	CAGTG	4 cut(s)	425, 679, 782, 821
Cac8I	GCNNGC	5 cut(s)	21, 167, 388, 403, 464
CaiI	CAGNNNCTG	1 cut(s)	377
CfoI	GCGC	2 cut(s)	53, 686
Cfr13I	GGNCC	2 cut(s)	82, 676
Csp6I	GTAC	4 cut(s)	26, 31, 322, 726
CviAII	CATG	5 cut(s)	20, 68, 166, 296, 545
CviQI	GTAC	4 cut(s)	26, 31, 322, 726
DdeI	CTNAG	6 cut(s)	290, 318, 370, 592, 843, 917
DpnI	GATC	1 cut(s)	443
DpnII	GATC	1 cut(s)	441
DraI	TTTAAA	1 cut(s)	63
EaeI	YGGCCR	1 cut(s)	460
EciI	GGCGGA	1 cut(s)	60
Ecl136II	GAGCTC	1 cut(s)	566
Eco24I	GRGCYC	1 cut(s)	568
Eco47I	GGWCC	2 cut(s)	82, 676
Eco53kI	GAGCTC	1 cut(s)	566
Eco57I	CTGAAG	4 cut(s)	384, 399, 806, 948
Eco72I	CACGTG	1 cut(s)	558
EcoICRI	GAGCTC	1 cut(s)	566
EcoRII	CCWGG	2 cut(s)	353, 698
EcoT22I	ATGCAT	1 cut(s)	21
EcoT38I	GRGCYC	1 cut(s)	568
FaeI	CATG	5 cut(s)	23, 71, 169, 299, 548
FaqI	GGGAC	2 cut(s)	68, 284
FatI	CATG	5 cut(s)	19, 67, 165, 295, 544
Fnu4HI	GCNGC	7 cut(s)	126, 129, 375, 460, 489, 636, 812
FokI	GGATG	2 cut(s)	680, 965
FriOI	GRGCYC	1 cut(s)	568
Fsp4HI	GCNGC	7 cut(s)	126, 129, 375, 460, 489, 636, 812
FspBI	CTAG	4 cut(s)	333, 524, 645, 942
FspI	TGCGCA	1 cut(s)	685
GlaI	GCGC	2 cut(s)	52, 685
GluI	GCNGC	7 cut(s)	126, 129, 375, 460, 489, 636, 812
GsaI	CCCAGC	1 cut(s)	441
GsuI	CTGGAG	1 cut(s)	243
HaeII	RGCGCY	1 cut(s)	54
HaeIII	GGCC	4 cut(s)	175, 358, 405, 462
HapII	CCGG	2 cut(s)	80, 266
HhaI	GCGC	2 cut(s)	53, 686
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	5 cut(s)	23, 71, 169, 299, 548
Hin6I	GCGC	2 cut(s)	51, 684
HinP1I	GCGC	2 cut(s)	51, 684
HincII	GTYRAC	1 cut(s)	705
HindII	GTYRAC	1 cut(s)	705
HindIII	AAGCTT	1 cut(s)	11
HinfI	GANTC	2 cut(s)	431, 902
HpaII	CCGG	2 cut(s)	80, 266
HphI	GGTGA	2 cut(s)	407, 883
Hpy166II	GTNNAC	4 cut(s)	311, 324, 679, 705
Hpy188I	TCNGA	6 cut(s)	159, 291, 642, 660, 831, 918
Hpy188III	TCNNGA	4 cut(s)	586, 611, 802, 889
Hpy8I	GTNNAC	4 cut(s)	311, 324, 679, 705
Hpy99I	CGWCG	1 cut(s)	910
HpyAV	CCTTC	7 cut(s)	91, 154, 374, 623, 730, 868, 872
HpyCH4III	ACNGT	3 cut(s)	30, 825, 895
HpyCH4IV	ACGT	2 cut(s)	557, 908
HpyCH4V	TGCA	5 cut(s)	19, 253, 386, 515, 782
HpyF10VI	GCNNNNNNNGC	5 cut(s)	383, 690, 779, 817, 932
HpyF3I	CTNAG	6 cut(s)	290, 318, 370, 592, 843, 917
HpySE526I	ACGT	2 cut(s)	557, 908
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	5 cut(s)	23, 71, 169, 299, 548
HspAI	GCGC	2 cut(s)	51, 684
Kzo9I	GATC	1 cut(s)	441
LmnI	GCTCC	3 cut(s)	82, 563, 632
Lsp1109I	GCAGC	6 cut(s)	115, 137, 386, 475, 647, 823
LweI	GCATC	2 cut(s)	702, 922
MaeI	CTAG	4 cut(s)	333, 524, 645, 942
MaeII	ACGT	2 cut(s)	557, 908
MaeIII	GTNAC	5 cut(s)	208, 280, 536, 547, 569
MalI	GATC	1 cut(s)	443
MboI	GATC	1 cut(s)	441
MboII	GAAGA	3 cut(s)	799, 817, 941
MhlI	GDGCHC	1 cut(s)	568
MluCI	AATT	4 cut(s)	153, 191, 578, 718
MlyI	GAGTC	1 cut(s)	896
MmeI	TCCRAC	3 cut(s)	220, 544, 638
Mph1103I	ATGCAT	1 cut(s)	21
MseI	TTAA	3 cut(s)	62, 864, 988
MslI	CAYNNNNRTG	1 cut(s)	300
MspA1I	CMGCKG	3 cut(s)	128, 377, 814
MspI	CCGG	2 cut(s)	80, 266
MspR9I	CCNGG	3 cut(s)	267, 355, 700
MvaI	CCWGG	2 cut(s)	355, 700
MvnI	CGCG	1 cut(s)	962
MwoI	GCNNNNNNNGC	5 cut(s)	383, 690, 779, 817, 932
NciI	CCSGG	1 cut(s)	267
NdeII	GATC	1 cut(s)	441
NlaIII	CATG	5 cut(s)	23, 71, 169, 299, 548
NlaIV	GGNNCC	3 cut(s)	78, 84, 819
NmuCI	GTSAC	1 cut(s)	280
NsbI	TGCGCA	1 cut(s)	685
NsiI	ATGCAT	1 cut(s)	21
NspI	RCATGY	4 cut(s)	23, 71, 169, 548
PaeI	GCATGC	2 cut(s)	23, 169
PfeI	GAWTC	1 cut(s)	431
PkrI	GCNGC	7 cut(s)	127, 130, 376, 461, 490, 637, 813
PleI	GAGTC	1 cut(s)	896
PmaCI	CACGTG	1 cut(s)	558
PmlI	CACGTG	1 cut(s)	558
PpsI	GAGTC	1 cut(s)	896
Ppu21I	YACGTR	1 cut(s)	558
Psp124BI	GAGCTC	1 cut(s)	568
Psp6I	CCWGG	2 cut(s)	353, 698
PspCI	CACGTG	1 cut(s)	558
PspFI	CCCAGC	1 cut(s)	437
PspGI	CCWGG	2 cut(s)	353, 698
PspN4I	GGNNCC	3 cut(s)	78, 84, 819
PspPI	GGNCC	2 cut(s)	82, 676
PstNI	CAGNNNCTG	1 cut(s)	377
PvuII	CAGCTG	3 cut(s)	128, 377, 814
RsaI	GTAC	4 cut(s)	27, 32, 323, 727
RsaNI	GTAC	4 cut(s)	26, 31, 322, 726
RseI	CAYNNNNRTG	1 cut(s)	300
SacI	GAGCTC	1 cut(s)	568
SaqAI	TTAA	3 cut(s)	62, 864, 988
SatI	GCNGC	7 cut(s)	126, 129, 375, 460, 489, 636, 812
Sau3AI	GATC	1 cut(s)	441
Sau96I	GGNCC	2 cut(s)	82, 676
SchI	GAGTC	1 cut(s)	896
ScrFI	CCNGG	3 cut(s)	267, 355, 700
SduI	GDGCHC	1 cut(s)	568
SfaNI	GCATC	2 cut(s)	702, 922
SinI	GGWCC	2 cut(s)	82, 676
SmiMI	CAYNNNNRTG	1 cut(s)	300
SmlI	CTYRAG	1 cut(s)	887
SmoI	CTYRAG	1 cut(s)	887
SphI	GCATGC	2 cut(s)	23, 169
Sse9I	AATT	4 cut(s)	153, 191, 578, 718
SsiI	CCGC	3 cut(s)	23, 45, 459
SspMI	CTAG	4 cut(s)	333, 524, 645, 942
SstI	GAGCTC	1 cut(s)	568
StyD4I	CCNGG	3 cut(s)	265, 353, 698
TaaI	ACNGT	3 cut(s)	30, 825, 895
TaiI	ACGT	2 cut(s)	560, 911
TaqI	TCGA	4 cut(s)	803, 900, 905, 981
TasI	AATT	4 cut(s)	153, 191, 578, 718
TatI	WGTACW	1 cut(s)	321
TauI	GCSGC	1 cut(s)	462
TfiI	GAWTC	1 cut(s)	431
Tru1I	TTAA	3 cut(s)	62, 864, 988
Tru9I	TTAA	3 cut(s)	62, 864, 988
TscAI	CASTG	4 cut(s)	425, 686, 789, 828
TseFI	GTSAC	1 cut(s)	280
TseI	GCWGC	6 cut(s)	125, 128, 374, 488, 635, 811
Tsp45I	GTSAC	1 cut(s)	280
TspGWI	ACGGA	2 cut(s)	443, 468
TspRI	CASTG	4 cut(s)	425, 686, 789, 828
VpaK11BI	GGWCC	2 cut(s)	82, 676
XapI	RAATTY	3 cut(s)	153, 191, 578
XceI	RCATGY	4 cut(s)	23, 71, 169, 548
XcmI	CCANNNNNNNNNTGG	1 cut(s)	686
XspI	CTAG	4 cut(s)	333, 524, 645, 942
Zsp2I	ATGCAT	1 cut(s)	21

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.