Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000313

Physical Location & Seq

Genomic Coordinates Reverse (-)

29783033 .. 29785905

Gene Structure 2873 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1167600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGGCCAATGAGACCATGCACGCTGTTCAGTACGACAACTATGGCGGCGGAACTTCTGGTTTAAAGCATGTTGAAGTTCCGATTCCAAATCCGAAAAAAGATGAGGTGTTGCTGAAATTGGAGGCAACTAGTCTAAACGCAATTGATTGGAAAGCTCAGAAAGGCATGCTCTGGCCTTTGTTGCCTCGCAGGTTTCCGTACATACCTGGTATTGATGTGGCAGGAGAGGTTGTAAAGGTAGGACCTGGAGTAAAAAAATTCAAACCTGGTGACAAAGTTGTCGGGAAGGTTAACACTCTTACTGGAGGTGGACTAGCTGAATTTGCCGTGACAATGGAGAGTTTAACTGTTGCAAGGCCACCGGAAGTTTCAGCAGCCACCGCTGTAGGATTACCCGTTGCTGGTCTCACAGCTCACCAGGCTCTTACACAAACTGCAGGGATCAAGCTTGATGGAAGTGGTCAGCAAGCAAACATCCTGATCACAGCTGCTTCAGGTGGTGTAGGTCTCTATGCGGTTCAGTTGGCAAAGCTCGGAAATACTCATATTACAGCCACTTGTGGTGCCCGCAACATTGAACTGGTCAAAAGCCTAGGGGCTGATGAGGTTATTGACTACAAGACCCCAGAAGGTGCAGCTCTTAGGAGTCCATCTGGTCGAAAATATGATGTTGTGATCCACTGTGCAACATGCATTCCTTGGTCAACTTTTGAGCCTAATTTGAGTGCGAACGGTAAGGTAATAGATGTTACTCCCACCCCCAGCTCTCTGTTTAGCGTTGCACTGAAGAAACTTACCTTTTCTAAGAAGCAACTGGTGCCACTGTTCTTATTTCCCATGGCTGATAACCTTGAGTATCTTATTAAGTTGGTGAAAGAAGGGAAGCTGAAGACTATAATTGACTCAAAGTATCCCCTGACCAAGGCGGAGGACGCTTGGGATAGGATCAGCGATGGCCATGCTACCGGAAAGATAATTGTGGAGCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N_2

Protein Analysis

330

Amino Acids

34.95

Weight (kDa)

8.9

Isoelectric Point (pI)

27.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	33 - 117	8.6e-16	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	167 - 230	5.2e-10	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	198 - 327	1.7e-23	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13010
fragaria_vesca	FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica	MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica	Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis	pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis	RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata	RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora	Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa	Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha	Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana	Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	278
Acc36I	ACCTGC	1 cut(s)	180
AccB1I	GGYRCC	2 cut(s)	563, 817
AciI	CCGC	6 cut(s)	45, 48, 381, 515, 568, 926
AclWI	GGATC	3 cut(s)	449, 670, 953
AcoI	YGGCCR	2 cut(s)	3, 955
AcsI	RAATTY	2 cut(s)	257, 320
AcuI	CTGAAG	3 cut(s)	477, 806, 908
AfaI	GTAC	2 cut(s)	32, 200
AfiI	CCNNNNNNNGG	2 cut(s)	401, 922
AgsI	TTSAA	3 cut(s)	74, 262, 578
AhlI	ACTAGT	1 cut(s)	128
AjnI	CCWGG	4 cut(s)	205, 244, 265, 417
AluBI	AGCT	9 cut(s)	155, 317, 413, 448, 488, 532, 638, 765, 886
AluI	AGCT	9 cut(s)	155, 317, 413, 448, 488, 532, 638, 765, 886
Alw26I	GTCTC	3 cut(s)	5, 410, 512
AlwI	GGATC	3 cut(s)	449, 670, 953
AoxI	GGCC	4 cut(s)	3, 173, 356, 955
ApeKI	GCWGC	3 cut(s)	374, 488, 635
ApoI	RAATTY	2 cut(s)	257, 320
AspA2I	CCTAGG	1 cut(s)	592
AspS9I	GGNCC	1 cut(s)	242
AsuHPI	GGTGA	3 cut(s)	281, 407, 883
AvaII	GGWCC	1 cut(s)	242
AvrII	CCTAGG	1 cut(s)	592
BaeGI	GKGCMC	1 cut(s)	568
BalI	TGGCCA	2 cut(s)	5, 957
BanI	GGYRCC	2 cut(s)	563, 817
BbsI	GAAGAC	1 cut(s)	896
BbvI	GCAGC	3 cut(s)	386, 475, 647
BccI	CCATC	3 cut(s)	446, 658, 947
BceAI	ACGGC	1 cut(s)	311
BcgI	CGANNNNNNTGC	2 cut(s)	941, 975
BciT130I	CCWGG	4 cut(s)	207, 246, 267, 419
BciVI	GTATCC	1 cut(s)	921
BclI	TGATCA	1 cut(s)	480
BcoDI	GTCTC	3 cut(s)	5, 410, 512
BcuI	ACTAGT	1 cut(s)	128
BfaI	CTAG	3 cut(s)	129, 314, 593
BfmI	CTRYAG	2 cut(s)	384, 435
BfuAI	ACCTGC	1 cut(s)	180
BfuI	GTATCC	1 cut(s)	921
BisI	GCNGC	4 cut(s)	46, 375, 489, 636
BlnI	CCTAGG	1 cut(s)	592
BlsI	GCNGC	4 cut(s)	47, 376, 490, 637
Bme1390I	CCNGG	4 cut(s)	207, 246, 267, 419
Bme18I	GGWCC	1 cut(s)	242
BmgT120I	GGNCC	1 cut(s)	242
BmiI	GGNNCC	3 cut(s)	565, 819, 984
BmrFI	CCNGG	4 cut(s)	207, 246, 267, 419
BpiI	GAAGAC	1 cut(s)	896
BpmI	CTGGAG	2 cut(s)	267, 324
BpuEI	CTTGAG	1 cut(s)	872
BsaI	GGTCTC	3 cut(s)	5, 410, 512
BsaJI	CCNNGG	4 cut(s)	592, 698, 837, 921
BsaWI	WCCGGW	2 cut(s)	361, 965
BsaXI	ACNNNNNCTCC	2 cut(s)	216, 246
Bsc4I	CCNNNNNNNGG	2 cut(s)	401, 922
Bse1I	ACTGG	3 cut(s)	307, 585, 819
BseBI	CCWGG	4 cut(s)	207, 246, 267, 419
BseDI	CCNNGG	4 cut(s)	592, 698, 837, 921
BseGI	GGATG	1 cut(s)	474
BseLI	CCNNNNNNNGG	2 cut(s)	401, 922
BseMII	CTCAG	1 cut(s)	170
BseNI	ACTGG	3 cut(s)	307, 585, 819
BseSI	GKGCMC	1 cut(s)	568
BseXI	GCAGC	3 cut(s)	386, 475, 647
BseYI	CCCAGC	1 cut(s)	761
BsgI	GTGCAG	1 cut(s)	654
BshFI	GGCC	4 cut(s)	5, 175, 358, 957
BshNI	GGYRCC	2 cut(s)	563, 817
BsiSI	CCGG	2 cut(s)	362, 966
BslI	CCNNNNNNNGG	2 cut(s)	401, 922
BsmAI	GTCTC	3 cut(s)	5, 410, 512
BsmI	GAATGC	1 cut(s)	693
BsnI	GGCC	4 cut(s)	5, 175, 358, 957
Bso31I	GGTCTC	3 cut(s)	5, 410, 512
Bsp1286I	GDGCHC	1 cut(s)	568
Bsp143I	GATC	4 cut(s)	441, 480, 675, 945
Bsp19I	CCATGG	1 cut(s)	837
BspACI	CCGC	6 cut(s)	45, 48, 381, 515, 568, 926
BspANI	GGCC	4 cut(s)	5, 175, 358, 957
BspCNI	CTCAG	1 cut(s)	169
BspLI	GGNNCC	3 cut(s)	565, 819, 984
BspMAI	CTGCAG	1 cut(s)	439
BspMI	ACCTGC	1 cut(s)	180
BspPI	GGATC	3 cut(s)	449, 670, 953
BspT107I	GGYRCC	2 cut(s)	563, 817
BspTNI	GGTCTC	3 cut(s)	5, 410, 512
BsrI	ACTGG	3 cut(s)	307, 585, 819
BssECI	CCNNGG	4 cut(s)	592, 698, 837, 921
BssMI	GATC	4 cut(s)	441, 480, 675, 945
BssT1I	CCWWGG	4 cut(s)	592, 698, 837, 921
Bst2UI	CCWGG	4 cut(s)	207, 246, 267, 419
Bst4CI	ACNGT	4 cut(s)	349, 683, 734, 825
BstAPI	GCANNNNNTGC	1 cut(s)	817
BstC8I	GCNNGC	4 cut(s)	21, 167, 468, 568
BstDEI	CTNAG	4 cut(s)	156, 641, 804, 987
BstDSI	CCRYGG	1 cut(s)	837
BstENI	CCTNNNNNAGG	1 cut(s)	920
BstF5I	GGATG	1 cut(s)	474
BstKTI	GATC	4 cut(s)	444, 483, 678, 948
BstMAI	GTCTC	3 cut(s)	5, 410, 512
BstMBI	GATC	4 cut(s)	441, 480, 675, 945
BstMWI	GCNNNNNNNGC	6 cut(s)	181, 323, 380, 419, 817, 932
BstNI	CCWGG	4 cut(s)	207, 246, 267, 419
BstNSI	RCATGY	3 cut(s)	71, 169, 693
BstSCI	CCNGG	4 cut(s)	205, 244, 265, 417
BstSFI	CTRYAG	2 cut(s)	384, 435
BstSLI	GKGCMC	1 cut(s)	568
BstV1I	GCAGC	3 cut(s)	386, 475, 647
BstV2I	GAAGAC	1 cut(s)	896
BsuI	GTATCC	1 cut(s)	921
BsuRI	GGCC	4 cut(s)	5, 175, 358, 957
BtgI	CCRYGG	1 cut(s)	837
BtgZI	GCGATG	1 cut(s)	966
BtsCI	GGATG	1 cut(s)	474
BtsIMutI	CAGTG	3 cut(s)	679, 782, 821
BveI	ACCTGC	1 cut(s)	180
Cac8I	GCNNGC	4 cut(s)	21, 167, 468, 568
Cfr13I	GGNCC	1 cut(s)	242
CseI	GACGC	1 cut(s)	941
CsiI	ACCWGGT	2 cut(s)	205, 265
Csp6I	GTAC	2 cut(s)	31, 199
CviAII	CATG	6 cut(s)	16, 68, 166, 690, 838, 959
CviQI	GTAC	2 cut(s)	31, 199
DdeI	CTNAG	4 cut(s)	156, 641, 804, 987
DpnI	GATC	4 cut(s)	443, 482, 677, 947
DpnII	GATC	4 cut(s)	441, 480, 675, 945
DraI	TTTAAA	1 cut(s)	63
DrdI	GACNNNNNNGTC	1 cut(s)	278
DseDI	GACNNNNNNGTC	1 cut(s)	278
EaeI	YGGCCR	2 cut(s)	3, 955
EciI	GGCGGA	2 cut(s)	63, 941
Eco130I	CCWWGG	4 cut(s)	592, 698, 837, 921
Eco31I	GGTCTC	3 cut(s)	5, 410, 512
Eco47I	GGWCC	1 cut(s)	242
Eco57I	CTGAAG	3 cut(s)	477, 806, 908
EcoNI	CCTNNNNNAGG	1 cut(s)	920
EcoO109I	RGGNCCY	1 cut(s)	242
EcoRII	CCWGG	4 cut(s)	205, 244, 265, 417
EcoT14I	CCWWGG	4 cut(s)	592, 698, 837, 921
EcoT22I	ATGCAT	1 cut(s)	695
ErhI	CCWWGG	4 cut(s)	592, 698, 837, 921
FaeI	CATG	6 cut(s)	19, 71, 169, 693, 841, 962
FatI	CATG	6 cut(s)	15, 67, 165, 689, 837, 958
FauI	CCCGC	1 cut(s)	575
FbaI	TGATCA	1 cut(s)	480
Fnu4HI	GCNGC	4 cut(s)	46, 375, 489, 636
FokI	GGATG	1 cut(s)	461
Fsp4HI	GCNGC	4 cut(s)	46, 375, 489, 636
FspBI	CTAG	3 cut(s)	129, 314, 593
GluI	GCNGC	4 cut(s)	46, 375, 489, 636
GsaI	CCCAGC	1 cut(s)	765
GsuI	CTGGAG	2 cut(s)	267, 324
HaeIII	GGCC	4 cut(s)	5, 175, 358, 957
HapII	CCGG	2 cut(s)	362, 966
HgaI	GACGC	1 cut(s)	941
Hin1II	CATG	6 cut(s)	19, 71, 169, 693, 841, 962
HincII	GTYRAC	2 cut(s)	292, 705
HindII	GTYRAC	2 cut(s)	292, 705
HindIII	AAGCTT	1 cut(s)	446
HinfI	GANTC	3 cut(s)	82, 646, 902
HpaI	GTTAAC	1 cut(s)	292
HpaII	CCGG	2 cut(s)	362, 966
HphI	GGTGA	3 cut(s)	281, 407, 883
Hpy166II	GTNNAC	3 cut(s)	292, 311, 705
Hpy188I	TCNGA	4 cut(s)	81, 93, 159, 536
Hpy188III	TCNNGA	2 cut(s)	283, 478
Hpy8I	GTNNAC	3 cut(s)	292, 311, 705
HpyAV	CCTTC	3 cut(s)	280, 623, 872
HpyCH4III	ACNGT	4 cut(s)	349, 683, 734, 825
HpyCH4V	TGCA	7 cut(s)	19, 353, 437, 635, 686, 693, 782
HpyF10VI	GCNNNNNNNGC	6 cut(s)	181, 323, 380, 419, 817, 932
HpyF3I	CTNAG	4 cut(s)	156, 641, 804, 987
Hsp92II	CATG	6 cut(s)	19, 71, 169, 693, 841, 962
Ksp22I	TGATCA	1 cut(s)	480
KspAI	GTTAAC	1 cut(s)	292
Kzo9I	GATC	4 cut(s)	441, 480, 675, 945
LmnI	GCTCC	1 cut(s)	982
Lsp1109I	GCAGC	3 cut(s)	386, 475, 647
MabI	ACCWGGT	2 cut(s)	205, 265
MaeI	CTAG	3 cut(s)	129, 314, 593
MaeIII	GTNAC	3 cut(s)	269, 328, 748
MalI	GATC	4 cut(s)	443, 482, 677, 947
MboI	GATC	4 cut(s)	441, 480, 675, 945
MboII	GAAGA	2 cut(s)	799, 901
MfeI	CAATTG	1 cut(s)	141
MhlI	GDGCHC	1 cut(s)	568
MlsI	TGGCCA	2 cut(s)	5, 957
MluCI	AATT	7 cut(s)	116, 141, 257, 320, 718, 897, 975
MluNI	TGGCCA	2 cut(s)	5, 957
MlyI	GAGTC	2 cut(s)	655, 896
MnlI	CCTC	7 cut(s)	97, 115, 195, 220, 299, 598, 922
Mox20I	TGGCCA	2 cut(s)	5, 957
Mph1103I	ATGCAT	1 cut(s)	695
MscI	TGGCCA	2 cut(s)	5, 957
MseI	TTAA	4 cut(s)	62, 291, 344, 864
Msp20I	TGGCCA	2 cut(s)	5, 957
MspA1I	CMGCKG	2 cut(s)	383, 488
MspI	CCGG	2 cut(s)	362, 966
MspR9I	CCNGG	4 cut(s)	207, 246, 267, 419
MunI	CAATTG	1 cut(s)	141
Mva1269I	GAATGC	1 cut(s)	693
MvaI	CCWGG	4 cut(s)	207, 246, 267, 419
MwoI	GCNNNNNNNGC	6 cut(s)	181, 323, 380, 419, 817, 932
NcoI	CCATGG	1 cut(s)	837
NdeII	GATC	4 cut(s)	441, 480, 675, 945
NlaIII	CATG	6 cut(s)	19, 71, 169, 693, 841, 962
NlaIV	GGNNCC	3 cut(s)	565, 819, 984
NmuCI	GTSAC	2 cut(s)	269, 328
NsiI	ATGCAT	1 cut(s)	695
NspI	RCATGY	3 cut(s)	71, 169, 693
PaeI	GCATGC	1 cut(s)	169
PctI	GAATGC	1 cut(s)	693
PfeI	GAWTC	1 cut(s)	82
PkrI	GCNGC	4 cut(s)	47, 376, 490, 637
PleI	GAGTC	2 cut(s)	654, 896
PpsI	GAGTC	2 cut(s)	654, 896
PpuMI	RGGWCCY	1 cut(s)	242
Psp5II	RGGWCCY	1 cut(s)	242
Psp6I	CCWGG	4 cut(s)	205, 244, 265, 417
PspFI	CCCAGC	1 cut(s)	761
PspGI	CCWGG	4 cut(s)	205, 244, 265, 417
PspN4I	GGNNCC	3 cut(s)	565, 819, 984
PspPI	GGNCC	1 cut(s)	242
PspPPI	RGGWCCY	1 cut(s)	242
PstI	CTGCAG	1 cut(s)	439
PvuII	CAGCTG	1 cut(s)	488
RsaI	GTAC	2 cut(s)	32, 200
RsaNI	GTAC	2 cut(s)	31, 199
SaqAI	TTAA	4 cut(s)	62, 291, 344, 864
SatI	GCNGC	4 cut(s)	46, 375, 489, 636
Sau3AI	GATC	4 cut(s)	441, 480, 675, 945
Sau96I	GGNCC	1 cut(s)	242
SchI	GAGTC	2 cut(s)	655, 896
ScrFI	CCNGG	4 cut(s)	207, 246, 267, 419
SduI	GDGCHC	1 cut(s)	568
SexAI	ACCWGGT	2 cut(s)	205, 265
SfcI	CTRYAG	2 cut(s)	384, 435
SinI	GGWCC	1 cut(s)	242
SmlI	CTYRAG	1 cut(s)	851
SmoI	CTYRAG	1 cut(s)	851
SpeI	ACTAGT	1 cut(s)	128
SphI	GCATGC	1 cut(s)	169
Sse9I	AATT	7 cut(s)	116, 141, 257, 320, 718, 897, 975
SsiI	CCGC	6 cut(s)	45, 48, 381, 515, 568, 926
SspMI	CTAG	3 cut(s)	129, 314, 593
StyD4I	CCNGG	4 cut(s)	205, 244, 265, 417
StyI	CCWWGG	4 cut(s)	592, 698, 837, 921
TaaI	ACNGT	4 cut(s)	349, 683, 734, 825
TaqI	TCGA	1 cut(s)	658
TasI	AATT	7 cut(s)	116, 141, 257, 320, 718, 897, 975
TauI	GCSGC	1 cut(s)	48
TfiI	GAWTC	1 cut(s)	82
Tru1I	TTAA	4 cut(s)	62, 291, 344, 864
Tru9I	TTAA	4 cut(s)	62, 291, 344, 864
TscAI	CASTG	3 cut(s)	686, 789, 828
TseFI	GTSAC	2 cut(s)	269, 328
TseI	GCWGC	3 cut(s)	374, 488, 635
Tsp45I	GTSAC	2 cut(s)	269, 328
TspGWI	ACGGA	1 cut(s)	186
TspRI	CASTG	3 cut(s)	686, 789, 828
VpaK11BI	GGWCC	1 cut(s)	242
XagI	CCTNNNNNAGG	1 cut(s)	920
XapI	RAATTY	2 cut(s)	257, 320
XceI	RCATGY	3 cut(s)	71, 169, 693
XmaJI	CCTAGG	1 cut(s)	592
XspI	CTAG	3 cut(s)	129, 314, 593
Zsp2I	ATGCAT	1 cut(s)	695

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.