RchiOBHm_Chr6g0283611

Quinone-oxidoreductase homolog, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000313
Physical Location & Seq
Forward (+)
46835487 .. 46838517
3031 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25431

Sequence Viewer

Length: 819 bp
ATGGCAGAAGTGGCTGCAAAGCTTATGCATGCTGTTCAGTATGAAAACTATGGTGGAGGGCTTTCTGGTTTGAAGCATGTAGAGATTCCAATCCCCAGTCCTAAGAGAGATGAGGTTTTAGTGAAATTAGAAGCAGCTAGCCTAAATCCAGCTGATTGGAAAATTCAGAAAGGCACGCTGTGTCCTCTTGTTCCACGCAAATTCCCTTTTATACCTGGATTTGATGTGGCTGGAGAGATTGTAGAGGTTGGAGAAAGAGTTAAGAAGTTCAAAGCCGGTGATAAAGTTGTGGCAGTGCTCCATCTTTTGGATGGAGGTGGACTGGCTGAGTTTGCTACAGCTACTGAAGGTGCAGGCTTACCTACTTGTGGTCTCACAGCTCACCAGGCTCTAGCACAAGTTGGAGAGACCGAACTTGATGGAACTGGCCAGCTGAAGAGCATACTCATTACTGGTGCTTCTGGTGGTGTTGGCCATTATGCAGTCCAACTAGCAAAGCAGGCGAACGCTCATGTGACAGTGACTTGTGGAGCTCGTAACATCGAATTTGTCAAGAGATTGGGGGCTGATGAAGTTCTCGACTACAGTACCCCAGATGGGGCAGCTTTAAGGAGTCCATCTGGTCAAAAGTATGATGTGAAGCTGGTGCCTTTGATCATAAATGCCAAGGCTGAAAACCTTGATTATCTTTTGAAGTTAGTGAAGGAAGGAAAGCTGAAGACAGTGATCGACTCAAAGCATTCTCTAAGCAAGGCTGAAGATGCCTGGGCTAAAAGTATGGATGGTCATGCTACTGGGAAGATCATTTTAGAGGCTTAA

Protein Analysis

272

Amino Acids

28.72

Weight (kDa)

6.71

Isoelectric Point (pI)

27.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 99 4.2e-17 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 156 - 215 3.2e-09 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 223 - 270 7.1e-11 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13010
fragaria_vesca FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 646
AccB7I CCANNNNNTGG 1 cut(s) 307
AcoI YGGCCR 2 cut(s) 427, 472
AcsI RAATTY 3 cut(s) 162, 200, 545
AcuI CTGAAG 4 cut(s) 366, 455, 737, 777
AdeI CACNNNGTG 1 cut(s) 180
AfaI GTAC 1 cut(s) 589
AfiI CCNNNNNNNGG 4 cut(s) 307, 368, 597, 598
AgsI TTSAA 3 cut(s) 73, 271, 694
AjnI CCWGG 3 cut(s) 214, 384, 764
Alw21I GWGCWC 2 cut(s) 300, 535
Alw26I GTCTC 2 cut(s) 377, 401
AlwNI CAGNNNCTG 1 cut(s) 344
AoxI GGCC 2 cut(s) 427, 472
ApeKI GCWGC 3 cut(s) 14, 134, 602
ApoI RAATTY 3 cut(s) 162, 200, 545
AsuHPI GGTGA 2 cut(s) 290, 374
AsuNHI GCTAGC 1 cut(s) 137
BaeI ACNNNNGTAYC 2 cut(s) 571, 604
BalI TGGCCA 2 cut(s) 429, 474
BanI GGYRCC 1 cut(s) 646
BanII GRGCYC 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 725
Bbv12I GWGCWC 2 cut(s) 300, 535
BbvI GCAGC 2 cut(s) 146, 614
BccI CCATC 6 cut(s) 305, 309, 413, 590, 625, 776
BciT130I CCWGG 3 cut(s) 216, 386, 766
BclI TGATCA 1 cut(s) 654
BcoDI GTCTC 2 cut(s) 377, 401
BfaI CTAG 3 cut(s) 138, 392, 491
BfmI CTRYAG 2 cut(s) 336, 583
BisI GCNGC 3 cut(s) 15, 135, 603
BlsI GCNGC 3 cut(s) 16, 136, 604
Bme1390I CCNGG 3 cut(s) 216, 386, 766
BmiI GGNNCC 1 cut(s) 648
BmrFI CCNGG 3 cut(s) 216, 386, 766
BmrI ACTGGG 2 cut(s) 90, 804
BmsI GCATC 1 cut(s) 751
BmtI GCTAGC 1 cut(s) 141
BmuI ACTGGG 2 cut(s) 90, 804
BpiI GAAGAC 1 cut(s) 725
BpmI CTGGAG 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 89
BsaI GGTCTC 2 cut(s) 377, 401
BsaJI CCNNGG 2 cut(s) 666, 765
BsaXI ACNNNNNCTCC 4 cut(s) 225, 243, 255, 273
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 368, 597, 598
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 5 cut(s) 96, 327, 430, 457, 799
Bse8I GATNNNNATC 1 cut(s) 89
BseBI CCWGG 3 cut(s) 216, 386, 766
BseDI CCNNGG 2 cut(s) 666, 765
BseGI GGATG 2 cut(s) 316, 787
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 4 cut(s) 307, 368, 597, 598
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 5 cut(s) 96, 327, 430, 457, 799
BseXI GCAGC 2 cut(s) 146, 614
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 2 cut(s) 429, 474
BshNI GGYRCC 1 cut(s) 646
BsiHKAI GWGCWC 2 cut(s) 300, 535
BsiSI CCGG 1 cut(s) 276
BslI CCNNNNNNNGG 4 cut(s) 307, 368, 597, 598
BsmAI GTCTC 2 cut(s) 377, 401
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 2 cut(s) 429, 474
Bso31I GGTCTC 2 cut(s) 377, 401
Bsp1286I GDGCHC 2 cut(s) 300, 535
Bsp143I GATC 3 cut(s) 654, 726, 801
BspANI GGCC 2 cut(s) 429, 474
BspCNI CTCAG 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 648
BspOI GCTAGC 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 646
BspTNI GGTCTC 2 cut(s) 377, 401
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 5 cut(s) 96, 327, 430, 457, 799
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 666, 765
BssMI GATC 3 cut(s) 654, 726, 801
BssT1I CCWWGG 1 cut(s) 666
Bst2UI CCWGG 3 cut(s) 216, 386, 766
Bst4CI ACNGT 3 cut(s) 520, 587, 724
Bst6I CTCTTC 1 cut(s) 431
BstC8I GCNNGC 6 cut(s) 30, 139, 176, 355, 431, 501
BstDEI CTNAG 3 cut(s) 102, 327, 746
BstF5I GGATG 2 cut(s) 316, 787
BstKTI GATC 3 cut(s) 657, 729, 804
BstMAI GTCTC 2 cut(s) 377, 401
BstMBI GATC 3 cut(s) 654, 726, 801
BstMWI GCNNNNNNNGC 5 cut(s) 11, 332, 386, 500, 761
BstNI CCWGG 3 cut(s) 216, 386, 766
BstNSI RCATGY 2 cut(s) 32, 80
BstSCI CCNGG 3 cut(s) 214, 384, 764
BstSFI CTRYAG 2 cut(s) 336, 583
BstV1I GCAGC 2 cut(s) 146, 614
BstV2I GAAGAC 1 cut(s) 725
BstXI CCANNNNNNTGG 1 cut(s) 156
BsuRI GGCC 2 cut(s) 429, 474
BtsCI GGATG 2 cut(s) 316, 787
BtsI GCAGTG 1 cut(s) 300
BtsIMutI CAGTG 3 cut(s) 300, 525, 729
Cac8I GCNNGC 6 cut(s) 30, 139, 176, 355, 431, 501
CaiI CAGNNNCTG 1 cut(s) 344
Cfr10I RCCGGY 1 cut(s) 275
Csp6I GTAC 1 cut(s) 588
CviAII CATG 4 cut(s) 29, 77, 512, 788
CviQI GTAC 1 cut(s) 588
DdeI CTNAG 3 cut(s) 102, 327, 746
DpnI GATC 3 cut(s) 656, 728, 803
DpnII GATC 3 cut(s) 654, 726, 801
DraIII CACNNNGTG 1 cut(s) 180
EaeI YGGCCR 2 cut(s) 427, 472
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Ecl136II GAGCTC 1 cut(s) 533
Eco130I CCWWGG 1 cut(s) 666
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 2 cut(s) 377, 401
Eco53kI GAGCTC 1 cut(s) 533
Eco57I CTGAAG 4 cut(s) 366, 455, 737, 777
EcoICRI GAGCTC 1 cut(s) 533
EcoRII CCWGG 3 cut(s) 214, 384, 764
EcoT14I CCWWGG 1 cut(s) 666
EcoT22I ATGCAT 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 535
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 4 cut(s) 32, 80, 515, 791
FatI CATG 4 cut(s) 28, 76, 511, 787
FbaI TGATCA 1 cut(s) 654
Fnu4HI GCNGC 3 cut(s) 15, 135, 603
FokI GGATG 2 cut(s) 323, 794
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 3 cut(s) 15, 135, 603
FspBI CTAG 3 cut(s) 138, 392, 491
GluI GCNGC 3 cut(s) 15, 135, 603
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 2 cut(s) 429, 474
HapII CCGG 1 cut(s) 276
Hin1II CATG 4 cut(s) 32, 80, 515, 791
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 3 cut(s) 85, 613, 731
HpaII CCGG 1 cut(s) 276
HphI GGTGA 2 cut(s) 290, 374
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 2 cut(s) 553, 578
Hpy8I GTNNAC 1 cut(s) 320
HpyAV CCTTC 3 cut(s) 341, 697, 701
HpyCH4III ACNGT 3 cut(s) 520, 587, 724
HpyCH4V TGCA 4 cut(s) 17, 28, 353, 482
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 332, 386, 500, 761
HpyF3I CTNAG 3 cut(s) 102, 327, 746
Hsp92II CATG 4 cut(s) 32, 80, 515, 791
Ksp22I TGATCA 1 cut(s) 654
Kzo9I GATC 3 cut(s) 654, 726, 801
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 2 cut(s) 303, 530
Lsp1109I GCAGC 2 cut(s) 146, 614
LweI GCATC 1 cut(s) 751
MaeI CTAG 3 cut(s) 138, 392, 491
MaeIII GTNAC 3 cut(s) 514, 520, 536
MalI GATC 3 cut(s) 656, 728, 803
MboI GATC 3 cut(s) 654, 726, 801
MboII GAAGA 4 cut(s) 448, 730, 770, 811
MhlI GDGCHC 2 cut(s) 300, 535
MlsI TGGCCA 2 cut(s) 429, 474
MluCI AATT 4 cut(s) 125, 162, 200, 545
MluNI TGGCCA 2 cut(s) 429, 474
MlyI GAGTC 2 cut(s) 622, 725
MmeI TCCRAC 3 cut(s) 229, 382, 511
MnlI CCTC 6 cut(s) 50, 106, 195, 238, 308, 805
Mox20I TGGCCA 2 cut(s) 429, 474
Mph1103I ATGCAT 1 cut(s) 30
MscI TGGCCA 2 cut(s) 429, 474
MseI TTAA 3 cut(s) 261, 608, 817
Msp20I TGGCCA 2 cut(s) 429, 474
MspA1I CMGCKG 2 cut(s) 152, 433
MspI CCGG 1 cut(s) 276
MspR9I CCNGG 3 cut(s) 216, 386, 766
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 3 cut(s) 216, 386, 766
MwoI GCNNNNNNNGC 5 cut(s) 11, 332, 386, 500, 761
NdeII GATC 3 cut(s) 654, 726, 801
NheI GCTAGC 1 cut(s) 137
NlaIII CATG 4 cut(s) 32, 80, 515, 791
NlaIV GGNNCC 1 cut(s) 648
NmuCI GTSAC 2 cut(s) 514, 520
NsiI ATGCAT 1 cut(s) 30
NspI RCATGY 2 cut(s) 32, 80
PaeI GCATGC 1 cut(s) 32
PciSI GCTCTTC 1 cut(s) 431
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 307
PkrI GCNGC 3 cut(s) 16, 136, 604
PleI GAGTC 2 cut(s) 621, 725
PpsI GAGTC 2 cut(s) 621, 725
Psp124BI GAGCTC 1 cut(s) 535
Psp6I CCWGG 3 cut(s) 214, 384, 764
PspGI CCWGG 3 cut(s) 214, 384, 764
PspN4I GGNNCC 1 cut(s) 648
PstNI CAGNNNCTG 1 cut(s) 344
PvuII CAGCTG 2 cut(s) 152, 433
RsaI GTAC 1 cut(s) 589
RsaNI GTAC 1 cut(s) 588
SacI GAGCTC 1 cut(s) 535
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 3 cut(s) 261, 608, 817
SatI GCNGC 3 cut(s) 15, 135, 603
Sau3AI GATC 3 cut(s) 654, 726, 801
SchI GAGTC 2 cut(s) 622, 725
ScrFI CCNGG 3 cut(s) 216, 386, 766
SduI GDGCHC 2 cut(s) 300, 535
SfaNI GCATC 1 cut(s) 751
SfcI CTRYAG 2 cut(s) 336, 583
SphI GCATGC 1 cut(s) 32
Sse9I AATT 4 cut(s) 125, 162, 200, 545
SspMI CTAG 3 cut(s) 138, 392, 491
SstI GAGCTC 1 cut(s) 535
StyD4I CCNGG 3 cut(s) 214, 384, 764
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 3 cut(s) 520, 587, 724
TaqI TCGA 3 cut(s) 543, 579, 729
TaqII GACCGA 1 cut(s) 425
TasI AATT 4 cut(s) 125, 162, 200, 545
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 3 cut(s) 261, 608, 817
Tru9I TTAA 3 cut(s) 261, 608, 817
TscAI CASTG 3 cut(s) 300, 525, 729
TseFI GTSAC 2 cut(s) 514, 520
TseI GCWGC 3 cut(s) 14, 134, 602
Tsp45I GTSAC 2 cut(s) 514, 520
TspDTI ATGAA 2 cut(s) 57, 585
TspRI CASTG 3 cut(s) 300, 525, 729
Van91I CCANNNNNTGG 1 cut(s) 307
XapI RAATTY 3 cut(s) 162, 200, 545
XceI RCATGY 2 cut(s) 32, 80
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XspI CTAG 3 cut(s) 138, 392, 491
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.