Rh6BG270900

Quinone-oxidoreductase homolog, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000313
Physical Location & Seq
Forward (+)
48941865 .. 48943847
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG270900.1

Sequence Viewer

Length: 1002 bp
ATGGCAGCCATGTCGAAGAAGCTTATGCATGCGGTTCAGTACGACGGCTATGGTGGAGGAACTTCTGGTTTAAAGCATGTTGAGGTCCCAATCCCAACTCCAATAAAAAATGAGGTTCTGCTGAAATTGGAAGCAGCTAGTCTAAATCCAGTTGATTGGAAAATTCAGAAAGGCCAGCTGCGGCCTCTTTTCCCATTGAATTTCCCTCTAATACCTGTAACGGACGTATCTGGAGAGATTGTAGAGGTTGGCGGAGGAGTCAAAAAGTTCAAAGTGGGAGACAAAGTTGTTATAATGCTCAACCCTTTTTACGGAGGTGGGTTGGCTGAGTTTGCAATCTCTAATAAGAGACTGATGGTTACTAGGCCACCTGAAGTTAGCGCAGCTGAAGGTGCGGGCTTACCTATTGCTGGTCTCACAGCTCACCAGGCTGTCACACAAGCTGCAGGGATCAAGCTCGATGGCACTGGCCAGAAGCAGAAGAACATATTGGTCACTGCGGCCTCTGGTGGAGTTGGTCACTACGCAGTCCAACTAGCAAAGCTTGGAAACACTCATGTTACCGCCACTTGTGGAGCTCAGAACATTGAATTGGTGAAGAGCTTAGGGGTGGATGAGGTTCTTGATTACAAGACCCCTGAAGGGGCTGCTCTAAAGAGTCCATCTGGTCGGAAATATGATGCCGTGATCCACTGTGCAACTGGCATTCCTTGGTCTACTTTTGAGCCTAACTTGAGTGAAAAGGGGAAGGTCATAGACATTACTCCTGGTCCTAGTGCCTTCTCTACTTGTGTTCTGAAGATGCTAACTTTCTCCAAAAAGAAGTTGGTGCCACTTATGTTGTATCCCAAGACTAAGAACCTGGATTATCTTGTGAAGCTGGTGAAGGAAGGAAAGCTCAAGACAGTGATCGACTCGAGGCATCCTCTGAGCAAGGCTGAAGATGCTTGGGCTAAGAGTATTGATGGCCATGCAACTGGGAAGATCATTGTGGAGCCTTAA

Protein Analysis

333

Amino Acids

35.38

Weight (kDa)

9.35

Isoelectric Point (pI)

27.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 118 1e-14 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 171 - 255 7.1e-10 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 202 - 331 1.4e-20 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13010
fragaria_vesca FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 829
AccI GTMKAC 1 cut(s) 716
AciI CCGC 6 cut(s) 32, 181, 252, 395, 500, 564
AclWI GGATC 2 cut(s) 458, 682
AcoI YGGCCR 2 cut(s) 469, 967
AcsI RAATTY 2 cut(s) 162, 199
AcuI CTGAAG 5 cut(s) 393, 408, 660, 818, 960
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 4 cut(s) 311, 410, 642, 643
AgsI TTSAA 3 cut(s) 199, 271, 590
AjnI CCWGG 3 cut(s) 426, 766, 861
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 575, 607
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 3 cut(s) 273, 343, 419
AlwI GGATC 2 cut(s) 458, 682
Ama87I CYCGRG 1 cut(s) 916
AoxI GGCC 6 cut(s) 172, 182, 365, 469, 501, 967
ApeKI GCWGC 6 cut(s) 5, 134, 178, 383, 443, 647
ApoI RAATTY 2 cut(s) 162, 199
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 85, 770
AsuHPI GGTGA 3 cut(s) 416, 607, 895
AvaI CYCGRG 1 cut(s) 916
AvaII GGWCC 2 cut(s) 85, 770
BaeI ACNNNNGTAYC 2 cut(s) 210, 243
BalI TGGCCA 2 cut(s) 471, 969
BanI GGYRCC 1 cut(s) 829
BanII GRGCYC 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 6 cut(s) 17, 146, 165, 395, 430, 634
BccI CCATC 4 cut(s) 349, 455, 670, 959
BceAI ACGGC 2 cut(s) 61, 668
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 3 cut(s) 428, 768, 863
BciVI GTATCC 1 cut(s) 855
BcoDI GTCTC 3 cut(s) 273, 343, 419
BfaI CTAG 4 cut(s) 138, 363, 536, 774
BfmI CTRYAG 1 cut(s) 444
BfuI GTATCC 1 cut(s) 855
BisI GCNGC 8 cut(s) 6, 135, 179, 182, 384, 444, 501, 648
BlsI GCNGC 8 cut(s) 7, 136, 180, 183, 385, 445, 502, 649
Bme1390I CCNGG 3 cut(s) 428, 768, 863
Bme18I GGWCC 2 cut(s) 85, 770
BmeT110I CYCGRG 1 cut(s) 916
BmgT120I GGNCC 2 cut(s) 85, 770
BmiI GGNNCC 3 cut(s) 87, 831, 996
BmrFI CCNGG 3 cut(s) 428, 768, 863
BmrI ACTGGG 1 cut(s) 987
BmsI GCATC 4 cut(s) 670, 792, 931, 934
BmuI ACTGGG 1 cut(s) 987
BplI GAGNNNNNCTC 2 cut(s) 910, 942
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 604
BpuEI CTTGAG 2 cut(s) 754, 884
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 1 cut(s) 710
BsaXI ACNNNNNCTCC 4 cut(s) 225, 255, 270, 300
Bsc4I CCNNNNNNNGG 4 cut(s) 311, 410, 642, 643
Bse1I ACTGG 4 cut(s) 149, 472, 706, 982
BseBI CCWGG 3 cut(s) 428, 768, 863
BseDI CCNNGG 1 cut(s) 710
BseGI GGATG 2 cut(s) 619, 922
BseLI CCNNNNNNNGG 4 cut(s) 311, 410, 642, 643
BseMII CTCAG 3 cut(s) 318, 593, 920
BseNI ACTGG 4 cut(s) 149, 472, 706, 982
BseRI GAGGAG 1 cut(s) 270
BseXI GCAGC 6 cut(s) 17, 146, 165, 395, 430, 634
BshFI GGCC 6 cut(s) 174, 184, 367, 471, 503, 969
BshNI GGYRCC 1 cut(s) 829
BsiHKAI GWGCWC 1 cut(s) 580
BsiHKCI CYCGRG 1 cut(s) 916
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 4 cut(s) 311, 410, 642, 643
BsmAI GTCTC 3 cut(s) 273, 343, 419
BsmFI GGGAC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 705
BsnI GGCC 6 cut(s) 174, 184, 367, 471, 503, 969
Bso31I GGTCTC 1 cut(s) 419
BsoBI CYCGRG 1 cut(s) 916
Bsp1286I GDGCHC 1 cut(s) 580
Bsp143I GATC 4 cut(s) 450, 687, 909, 984
BspACI CCGC 6 cut(s) 32, 181, 252, 395, 500, 564
BspANI GGCC 6 cut(s) 174, 184, 367, 471, 503, 969
BspCNI CTCAG 3 cut(s) 319, 592, 921
BspLI GGNNCC 3 cut(s) 87, 831, 996
BspMAI CTGCAG 1 cut(s) 448
BspPI GGATC 2 cut(s) 458, 682
BspQI GCTCTTC 1 cut(s) 593
BspT107I GGYRCC 1 cut(s) 829
BspTNI GGTCTC 1 cut(s) 419
BsrI ACTGG 4 cut(s) 149, 472, 706, 982
BssECI CCNNGG 1 cut(s) 710
BssMI GATC 4 cut(s) 450, 687, 909, 984
BssT1I CCWWGG 1 cut(s) 710
Bst2UI CCWGG 3 cut(s) 428, 768, 863
Bst4CI ACNGT 2 cut(s) 695, 907
Bst6I CTCTTC 1 cut(s) 593
BstC8I GCNNGC 3 cut(s) 30, 176, 397
BstDEI CTNAG 6 cut(s) 327, 579, 604, 855, 929, 954
BstF5I GGATG 2 cut(s) 619, 922
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 4 cut(s) 453, 690, 912, 987
BstMAI GTCTC 3 cut(s) 273, 343, 419
BstMBI GATC 4 cut(s) 450, 687, 909, 984
BstMWI GCNNNNNNNGC 4 cut(s) 332, 392, 428, 944
BstNI CCWGG 3 cut(s) 428, 768, 863
BstNSI RCATGY 2 cut(s) 32, 80
BstSCI CCNGG 3 cut(s) 426, 766, 861
BstSFI CTRYAG 1 cut(s) 444
BstV1I GCAGC 6 cut(s) 17, 146, 165, 395, 430, 634
BstXI CCANNNNNNTGG 2 cut(s) 156, 977
BsuI GTATCC 1 cut(s) 855
BsuRI GGCC 6 cut(s) 174, 184, 367, 471, 503, 969
BtsCI GGATG 2 cut(s) 619, 922
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 4 cut(s) 465, 495, 691, 912
Cac8I GCNNGC 3 cut(s) 30, 176, 397
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 85, 770
Csp6I GTAC 1 cut(s) 40
CspCI CAANNNNNGTGG 2 cut(s) 822, 857
CviAII CATG 5 cut(s) 10, 29, 77, 557, 971
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 6 cut(s) 327, 579, 604, 855, 929, 954
DpnI GATC 4 cut(s) 452, 689, 911, 986
DpnII GATC 4 cut(s) 450, 687, 909, 984
DraI TTTAAA 1 cut(s) 72
EaeI YGGCCR 2 cut(s) 469, 967
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
EciI GGCGGA 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 578
Eco130I CCWWGG 1 cut(s) 710
Eco24I GRGCYC 1 cut(s) 580
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 85, 770
Eco53kI GAGCTC 1 cut(s) 578
Eco57I CTGAAG 5 cut(s) 393, 408, 660, 818, 960
Eco88I CYCGRG 1 cut(s) 916
EcoICRI GAGCTC 1 cut(s) 578
EcoO109I RGGNCCY 1 cut(s) 85
EcoRII CCWGG 3 cut(s) 426, 766, 861
EcoT14I CCWWGG 1 cut(s) 710
EcoT22I ATGCAT 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 710
FaeI CATG 5 cut(s) 13, 32, 80, 560, 974
FaqI GGGAC 1 cut(s) 71
FatI CATG 5 cut(s) 9, 28, 76, 556, 970
FauI CCCGC 1 cut(s) 388
FblI GTMKAC 1 cut(s) 716
Fnu4HI GCNGC 8 cut(s) 6, 135, 179, 182, 384, 444, 501, 648
FokI GGATG 2 cut(s) 626, 909
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 8 cut(s) 6, 135, 179, 182, 384, 444, 501, 648
FspBI CTAG 4 cut(s) 138, 363, 536, 774
GlaI GCGC 1 cut(s) 382
GluI GCNGC 8 cut(s) 6, 135, 179, 182, 384, 444, 501, 648
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 6 cut(s) 174, 184, 367, 471, 503, 969
HhaI GCGC 1 cut(s) 383
Hin1II CATG 5 cut(s) 13, 32, 80, 560, 974
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HindIII AAGCTT 2 cut(s) 20, 542
HinfI GANTC 3 cut(s) 258, 658, 914
HphI GGTGA 3 cut(s) 416, 607, 895
Hpy166II GTNNAC 1 cut(s) 717
Hpy188I TCNGA 5 cut(s) 168, 582, 672, 798, 930
Hpy188III TCNNGA 3 cut(s) 231, 623, 901
Hpy8I GTNNAC 1 cut(s) 717
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 6 cut(s) 383, 635, 742, 790, 880, 884
HpyCH4III ACNGT 2 cut(s) 695, 907
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 5 cut(s) 28, 335, 446, 698, 974
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 392, 428, 944
HpyF3I CTNAG 6 cut(s) 327, 579, 604, 855, 929, 954
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 5 cut(s) 13, 32, 80, 560, 974
HspAI GCGC 1 cut(s) 381
Kzo9I GATC 4 cut(s) 450, 687, 909, 984
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 2 cut(s) 575, 994
Lsp1109I GCAGC 6 cut(s) 17, 146, 165, 395, 430, 634
LweI GCATC 4 cut(s) 670, 792, 931, 934
MaeI CTAG 4 cut(s) 138, 363, 536, 774
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 6 cut(s) 217, 358, 433, 493, 518, 559
MalI GATC 4 cut(s) 452, 689, 911, 986
MboI GATC 4 cut(s) 450, 687, 909, 984
MboII GAAGA 6 cut(s) 28, 493, 610, 811, 953, 994
MhlI GDGCHC 1 cut(s) 580
MlsI TGGCCA 2 cut(s) 471, 969
MluCI AATT 4 cut(s) 125, 162, 199, 590
MluNI TGGCCA 2 cut(s) 471, 969
MlyI GAGTC 3 cut(s) 267, 667, 908
MmeI TCCRAC 2 cut(s) 556, 650
Mox20I TGGCCA 2 cut(s) 471, 969
Mph1103I ATGCAT 1 cut(s) 30
MscI TGGCCA 2 cut(s) 471, 969
MseI TTAA 2 cut(s) 71, 1000
Msp20I TGGCCA 2 cut(s) 471, 969
MspA1I CMGCKG 2 cut(s) 178, 386
MspR9I CCNGG 3 cut(s) 428, 768, 863
Mva1269I GAATGC 1 cut(s) 705
MvaI CCWGG 3 cut(s) 428, 768, 863
MwoI GCNNNNNNNGC 4 cut(s) 332, 392, 428, 944
NdeII GATC 4 cut(s) 450, 687, 909, 984
NlaIII CATG 5 cut(s) 13, 32, 80, 560, 974
NlaIV GGNNCC 3 cut(s) 87, 831, 996
NmuCI GTSAC 3 cut(s) 433, 493, 518
NsiI ATGCAT 1 cut(s) 30
NspI RCATGY 2 cut(s) 32, 80
PaeI GCATGC 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 916
PciSI GCTCTTC 1 cut(s) 593
PctI GAATGC 1 cut(s) 705
PkrI GCNGC 8 cut(s) 7, 136, 180, 183, 385, 445, 502, 649
PleI GAGTC 3 cut(s) 266, 666, 908
PpsI GAGTC 3 cut(s) 266, 666, 908
PpuMI RGGWCCY 1 cut(s) 85
PsiI TTATAA 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 580
Psp5II RGGWCCY 1 cut(s) 85
Psp6I CCWGG 3 cut(s) 426, 766, 861
PspGI CCWGG 3 cut(s) 426, 766, 861
PspN4I GGNNCC 3 cut(s) 87, 831, 996
PspPI GGNCC 2 cut(s) 85, 770
PspPPI RGGWCCY 1 cut(s) 85
PspXI VCTCGAGB 1 cut(s) 916
PstI CTGCAG 1 cut(s) 448
PvuII CAGCTG 2 cut(s) 178, 386
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SacI GAGCTC 1 cut(s) 580
SapI GCTCTTC 1 cut(s) 593
SaqAI TTAA 2 cut(s) 71, 1000
SatI GCNGC 8 cut(s) 6, 135, 179, 182, 384, 444, 501, 648
Sau3AI GATC 4 cut(s) 450, 687, 909, 984
Sau96I GGNCC 2 cut(s) 85, 770
SchI GAGTC 3 cut(s) 267, 667, 908
ScrFI CCNGG 3 cut(s) 428, 768, 863
SduI GDGCHC 1 cut(s) 580
SfaNI GCATC 4 cut(s) 670, 792, 931, 934
SfcI CTRYAG 1 cut(s) 444
Sfr274I CTCGAG 1 cut(s) 916
SinI GGWCC 2 cut(s) 85, 770
SlaI CTCGAG 1 cut(s) 916
SmlI CTYRAG 3 cut(s) 733, 899, 916
SmoI CTYRAG 3 cut(s) 733, 899, 916
SphI GCATGC 1 cut(s) 32
Sse9I AATT 4 cut(s) 125, 162, 199, 590
SsiI CCGC 6 cut(s) 32, 181, 252, 395, 500, 564
SspMI CTAG 4 cut(s) 138, 363, 536, 774
SstI GAGCTC 1 cut(s) 580
StyD4I CCNGG 3 cut(s) 426, 766, 861
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 2 cut(s) 695, 907
TaiI ACGT 1 cut(s) 228
TaqI TCGA 4 cut(s) 14, 459, 912, 917
TasI AATT 4 cut(s) 125, 162, 199, 590
TauI GCSGC 2 cut(s) 184, 503
Tru1I TTAA 2 cut(s) 71, 1000
Tru9I TTAA 2 cut(s) 71, 1000
TscAI CASTG 4 cut(s) 472, 502, 698, 912
TseFI GTSAC 3 cut(s) 433, 493, 518
TseI GCWGC 6 cut(s) 5, 134, 178, 383, 443, 647
Tsp45I GTSAC 3 cut(s) 433, 493, 518
TspGWI ACGGA 2 cut(s) 236, 327
TspRI CASTG 4 cut(s) 472, 502, 698, 912
VpaK11BI GGWCC 2 cut(s) 85, 770
XapI RAATTY 2 cut(s) 162, 199
XceI RCATGY 2 cut(s) 32, 80
XcmI CCANNNNNNNNNTGG 2 cut(s) 698, 823
XhoI CTCGAG 1 cut(s) 916
XmiI GTMKAC 1 cut(s) 716
XspI CTAG 4 cut(s) 138, 363, 536, 774
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.