Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0000313

Physical Location & Seq

Genomic Coordinates Forward (+)

44495964 .. 44497438

Gene Structure 1475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG270400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 993 bp

ATGGCAGCAAAGCTTATGCATGCGGTTCAGTACGACAGTTATGGTGGAGGACCTTCTGGTTTAAAGCATGTTCAAGTTCCAATCCCATCTCCAAAGAAAAATGAGGTTTTGCTCAAGTTGGAAGCAGCAAGTCTAAATCCAGGTGATTGTAAGATTCAGAAAGGCATGGCGTGGCCTCTTTTCCCTCGCAAGTTGCCTCACATTCCTGTAACTGATGTGGCTGGAGAGGTTGTAGAGGTGGGACTGGGAGTCACAAAGTTCAAAGTGGGTGACAAAGTTGTGGCAATGCTCAGCTATGGTAATGGAGGTGGACTTGGTGAGTTTGCGGCAGCTAATGAGAAATTGGTAGTTTCTAGGCCTCTTGAAGTGTCAGCAGCAGACGGCGCAGGCTTACCGGTTGCTGCTCTTACAGCTCACCAGGCTCTCACCAAAGATGCAGGGATCAAGCTTGATGGAACAAGCCAGCAGAAGAACATACTCATTACCGGTGCCTCAGGTAACGTCGGTCTCTATGCAATCCAACTAGCAAAGCTCGGAAATGCTCATGTCACCGCCACTTGTGGAGCTCGTAACATTGAACTAGTCAAGAGCTTAGGGGCAGATGAGGTTCTTGACTACAAGACCCCTGAAGGGGCAGCTGTCAAGAGCCCATCTGGTCGGACATATGATTTTGTGATCCATTCTTCATCAAAAGCAATTCCTTGGTCAGTCTTTGAGCCGAATTTGAGCTCAAATGGGAAAGTTATAGACCTTACTGCCGGCCTCAGCTCCTTGTTTACTTTTGTTCTTAAGAAACTCACCTTCTCCAAGAAGAAGCTGGTGCCATTGATCATAAATGCGAAGGGTGAGAACCTGGATTGTCTTGTTAAGTTGGTGAAGGAAGGAAAGCTCAAGACTGTGACTGACTCAAATTTTCCTCTAAGCAAAGCTGAAGATGCTTGGGCTAAAAGACTTGATGGCTACAATACAGGGAAGATCATTGTGGAGCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N_2

Protein Analysis

330

Amino Acids

34.77

Weight (kDa)

9.24

Isoelectric Point (pI)

22.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	33 - 118	1.2e-14	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	168 - 279	2.5e-09	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	198 - 328	2.9e-16	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13010
fragaria_vesca	FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica	MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica	Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis	pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis	RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata	RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora	Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa	Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha	Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana	Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	488, 820
AciI	CCGC	3 cut(s)	23, 326, 552
AclWI	GGATC	2 cut(s)	449, 670
AcsI	RAATTY	2 cut(s)	721, 910
AcuI	CTGAAG	2 cut(s)	648, 951
AfaI	GTAC	1 cut(s)	32
AfiI	CCNNNNNNNGG	2 cut(s)	630, 631
AflII	CTTAAG	1 cut(s)	788
AgeI	ACCGGT	2 cut(s)	394, 485
AgsI	TTSAA	4 cut(s)	74, 262, 365, 578
AhdI	GACNNNNNGTC	1 cut(s)	248
AhlI	ACTAGT	1 cut(s)	580
AjnI	CCWGG	3 cut(s)	139, 417, 852
Alw21I	GWGCWC	2 cut(s)	568, 731
Alw26I	GTCTC	1 cut(s)	512
AlwI	GGATC	2 cut(s)	449, 670
AoxI	GGCC	3 cut(s)	173, 356, 760
ApeKI	GCWGC	6 cut(s)	5, 125, 329, 374, 401, 635
ApoI	RAATTY	2 cut(s)	721, 910
AsiGI	ACCGGT	2 cut(s)	394, 485
AspLEI	GCGC	1 cut(s)	386
AspS9I	GGNCC	1 cut(s)	50
AsuHPI	GGTGA	9 cut(s)	155, 281, 329, 407, 418, 541, 790, 857, 886
AvaII	GGWCC	1 cut(s)	50
AxyI	CCTNAGG	1 cut(s)	493
BanI	GGYRCC	2 cut(s)	488, 820
BanII	GRGCYC	3 cut(s)	568, 650, 731
Bbv12I	GWGCWC	2 cut(s)	568, 731
BbvCI	CCTCAGC	1 cut(s)	764
BbvI	GCAGC	6 cut(s)	17, 137, 341, 386, 388, 647
BccI	CCATC	4 cut(s)	94, 446, 658, 950
BceAI	ACGGC	1 cut(s)	397
BciT130I	CCWGG	3 cut(s)	141, 419, 854
BclI	TGATCA	1 cut(s)	828
BcoDI	GTCTC	1 cut(s)	512
BcuI	ACTAGT	1 cut(s)	580
BfaI	CTAG	3 cut(s)	354, 524, 581
BfrI	CTTAAG	1 cut(s)	788
BisI	GCNGC	7 cut(s)	6, 126, 327, 330, 375, 402, 636
BlpI	GCTNAGC	1 cut(s)	290
BlsI	GCNGC	7 cut(s)	7, 127, 328, 331, 376, 403, 637
Bme1390I	CCNGG	3 cut(s)	141, 419, 854
Bme18I	GGWCC	1 cut(s)	50
BmeRI	GACNNNNNGTC	1 cut(s)	248
BmgT120I	GGNCC	1 cut(s)	50
BmiI	GGNNCC	2 cut(s)	490, 822
BmrFI	CCNGG	3 cut(s)	141, 419, 854
BmrI	ACTGGG	1 cut(s)	254
BmsI	GCATC	2 cut(s)	424, 925
BmuI	ACTGGG	1 cut(s)	254
BpmI	CTGGAG	1 cut(s)	243
Bpu10I	CCTNAGC	2 cut(s)	592, 764
Bpu1102I	GCTNAGC	1 cut(s)	290
BpuEI	CTTGAG	2 cut(s)	98, 875
BsaI	GGTCTC	1 cut(s)	512
BsaJI	CCNNGG	1 cut(s)	701
BsaWI	WCCGGW	2 cut(s)	394, 485
BsaXI	ACNNNNNCTCC	2 cut(s)	216, 246
Bsc4I	CCNNNNNNNGG	2 cut(s)	630, 631
Bse118I	RCCGGY	3 cut(s)	394, 485, 758
Bse1I	ACTGG	1 cut(s)	249
Bse21I	CCTNAGG	1 cut(s)	493
Bse3DI	GCAATG	1 cut(s)	291
BseBI	CCWGG	3 cut(s)	141, 419, 854
BseDI	CCNNGG	1 cut(s)	701
BseLI	CCNNNNNNNGG	2 cut(s)	630, 631
BseMI	GCAATG	1 cut(s)	291
BseMII	CTCAG	3 cut(s)	304, 507, 778
BseNI	ACTGG	1 cut(s)	249
BseXI	GCAGC	6 cut(s)	17, 137, 341, 386, 388, 647
BshFI	GGCC	3 cut(s)	175, 358, 762
BshNI	GGYRCC	2 cut(s)	488, 820
BshTI	ACCGGT	2 cut(s)	394, 485
BsiHKAI	GWGCWC	2 cut(s)	568, 731
BsiSI	CCGG	3 cut(s)	395, 486, 759
BslFI	GGGAC	1 cut(s)	255
BslI	CCNNNNNNNGG	2 cut(s)	630, 631
BsmAI	GTCTC	1 cut(s)	512
BsmFI	GGGAC	1 cut(s)	255
BsnI	GGCC	3 cut(s)	175, 358, 762
Bso31I	GGTCTC	1 cut(s)	512
Bsp1286I	GDGCHC	3 cut(s)	568, 650, 731
Bsp143I	GATC	4 cut(s)	441, 675, 828, 975
Bsp1720I	GCTNAGC	1 cut(s)	290
BspACI	CCGC	3 cut(s)	23, 326, 552
BspANI	GGCC	3 cut(s)	175, 358, 762
BspCNI	CTCAG	3 cut(s)	303, 506, 777
BspLI	GGNNCC	2 cut(s)	490, 822
BspPI	GGATC	2 cut(s)	449, 670
BspT107I	GGYRCC	2 cut(s)	488, 820
BspTI	CTTAAG	1 cut(s)	788
BspTNI	GGTCTC	1 cut(s)	512
BsrDI	GCAATG	1 cut(s)	291
BsrFI	RCCGGY	3 cut(s)	394, 485, 758
BsrI	ACTGG	1 cut(s)	249
BssAI	RCCGGY	3 cut(s)	394, 485, 758
BssECI	CCNNGG	1 cut(s)	701
BssMI	GATC	4 cut(s)	441, 675, 828, 975
BssT1I	CCWWGG	1 cut(s)	701
Bst2UI	CCWGG	3 cut(s)	141, 419, 854
Bst4CI	ACNGT	2 cut(s)	38, 898
BstAFI	CTTAAG	1 cut(s)	788
BstC8I	GCNNGC	4 cut(s)	21, 388, 464, 760
BstDEI	CTNAG	5 cut(s)	290, 493, 592, 764, 920
BstHHI	GCGC	1 cut(s)	386
BstKTI	GATC	4 cut(s)	444, 678, 831, 978
BstMAI	GTCTC	1 cut(s)	512
BstMBI	GATC	4 cut(s)	441, 675, 828, 975
BstMWI	GCNNNNNNNGC	4 cut(s)	383, 410, 419, 935
BstNI	CCWGG	3 cut(s)	141, 419, 854
BstNSI	RCATGY	2 cut(s)	23, 71
BstSCI	CCNGG	3 cut(s)	139, 417, 852
BstV1I	GCAGC	6 cut(s)	17, 137, 341, 386, 388, 647
Bsu36I	CCTNAGG	1 cut(s)	493
BsuRI	GGCC	3 cut(s)	175, 358, 762
Cac8I	GCNNGC	4 cut(s)	21, 388, 464, 760
CfoI	GCGC	1 cut(s)	386
Cfr10I	RCCGGY	3 cut(s)	394, 485, 758
Cfr13I	GGNCC	1 cut(s)	50
Csp6I	GTAC	1 cut(s)	31
CspAI	ACCGGT	2 cut(s)	394, 485
CviAII	CATG	4 cut(s)	20, 68, 166, 545
CviQI	GTAC	1 cut(s)	31
DdeI	CTNAG	5 cut(s)	290, 493, 592, 764, 920
DpnI	GATC	4 cut(s)	443, 677, 830, 977
DpnII	GATC	4 cut(s)	441, 675, 828, 975
DraI	TTTAAA	1 cut(s)	63
DriI	GACNNNNNGTC	1 cut(s)	248
Eam1105I	GACNNNNNGTC	1 cut(s)	248
Ecl136II	GAGCTC	2 cut(s)	566, 729
Eco130I	CCWWGG	1 cut(s)	701
Eco147I	AGGCCT	1 cut(s)	358
Eco24I	GRGCYC	3 cut(s)	568, 650, 731
Eco31I	GGTCTC	1 cut(s)	512
Eco47I	GGWCC	1 cut(s)	50
Eco53kI	GAGCTC	2 cut(s)	566, 729
Eco57I	CTGAAG	2 cut(s)	648, 951
Eco81I	CCTNAGG	1 cut(s)	493
EcoICRI	GAGCTC	2 cut(s)	566, 729
EcoO109I	RGGNCCY	1 cut(s)	50
EcoRII	CCWGG	3 cut(s)	139, 417, 852
EcoT14I	CCWWGG	1 cut(s)	701
EcoT22I	ATGCAT	1 cut(s)	21
EcoT38I	GRGCYC	3 cut(s)	568, 650, 731
ErhI	CCWWGG	1 cut(s)	701
FaeI	CATG	4 cut(s)	23, 71, 169, 548
FaqI	GGGAC	1 cut(s)	255
FatI	CATG	4 cut(s)	19, 67, 165, 544
FauNDI	CATATG	1 cut(s)	664
FbaI	TGATCA	1 cut(s)	828
Fnu4HI	GCNGC	7 cut(s)	6, 126, 327, 330, 375, 402, 636
FriOI	GRGCYC	3 cut(s)	568, 650, 731
Fsp4HI	GCNGC	7 cut(s)	6, 126, 327, 330, 375, 402, 636
FspBI	CTAG	3 cut(s)	354, 524, 581
GlaI	GCGC	1 cut(s)	385
GluI	GCNGC	7 cut(s)	6, 126, 327, 330, 375, 402, 636
GsuI	CTGGAG	1 cut(s)	243
HaeIII	GGCC	3 cut(s)	175, 358, 762
HapII	CCGG	3 cut(s)	395, 486, 759
HhaI	GCGC	1 cut(s)	386
Hin1II	CATG	4 cut(s)	23, 71, 169, 548
Hin6I	GCGC	1 cut(s)	384
HinP1I	GCGC	1 cut(s)	384
HindIII	AAGCTT	2 cut(s)	11, 446
HinfI	GANTC	3 cut(s)	154, 249, 905
HpaII	CCGG	3 cut(s)	395, 486, 759
HphI	GGTGA	9 cut(s)	155, 281, 329, 407, 418, 541, 790, 857, 886
Hpy166II	GTNNAC	2 cut(s)	311, 777
Hpy188I	TCNGA	3 cut(s)	159, 536, 660
Hpy188III	TCNNGA	5 cut(s)	362, 586, 611, 643, 892
Hpy8I	GTNNAC	2 cut(s)	311, 777
Hpy99I	CGWCG	1 cut(s)	506
HpyAV	CCTTC	6 cut(s)	63, 623, 811, 835, 871, 875
HpyCH4III	ACNGT	2 cut(s)	38, 898
HpyCH4IV	ACGT	1 cut(s)	501
HpyCH4V	TGCA	3 cut(s)	19, 437, 515
HpyF10VI	GCNNNNNNNGC	4 cut(s)	383, 410, 419, 935
HpyF3I	CTNAG	5 cut(s)	290, 493, 592, 764, 920
HpySE526I	ACGT	1 cut(s)	501
Hsp92II	CATG	4 cut(s)	23, 71, 169, 548
HspAI	GCGC	1 cut(s)	384
KroI	GCCGGC	1 cut(s)	758
KroNI	GCCGGC	1 cut(s)	760
Ksp22I	TGATCA	1 cut(s)	828
Kzo9I	GATC	4 cut(s)	441, 675, 828, 975
LmnI	GCTCC	3 cut(s)	563, 773, 985
Lsp1109I	GCAGC	6 cut(s)	17, 137, 341, 386, 388, 647
LweI	GCATC	2 cut(s)	424, 925
MaeI	CTAG	3 cut(s)	354, 524, 581
MaeII	ACGT	1 cut(s)	501
MaeIII	GTNAC	7 cut(s)	208, 250, 269, 497, 547, 569, 898
MalI	GATC	4 cut(s)	443, 677, 830, 977
MboI	GATC	4 cut(s)	441, 675, 828, 975
MboII	GAAGA	5 cut(s)	481, 675, 823, 944, 985
MhlI	GDGCHC	3 cut(s)	568, 650, 731
MluCI	AATT	4 cut(s)	341, 696, 721, 910
MlyI	GAGTC	2 cut(s)	258, 899
MmeI	TCCRAC	3 cut(s)	99, 544, 638
Mph1103I	ATGCAT	1 cut(s)	21
MroNI	GCCGGC	1 cut(s)	758
MseI	TTAA	3 cut(s)	62, 789, 867
MspA1I	CMGCKG	1 cut(s)	638
MspCI	CTTAAG	1 cut(s)	788
MspI	CCGG	3 cut(s)	395, 486, 759
MspR9I	CCNGG	3 cut(s)	141, 419, 854
MvaI	CCWGG	3 cut(s)	141, 419, 854
MwoI	GCNNNNNNNGC	4 cut(s)	383, 410, 419, 935
NaeI	GCCGGC	1 cut(s)	760
NdeI	CATATG	1 cut(s)	664
NdeII	GATC	4 cut(s)	441, 675, 828, 975
NgoMIV	GCCGGC	1 cut(s)	758
NlaIII	CATG	4 cut(s)	23, 71, 169, 548
NlaIV	GGNNCC	2 cut(s)	490, 822
NmuCI	GTSAC	4 cut(s)	250, 269, 547, 898
NsiI	ATGCAT	1 cut(s)	21
NspI	RCATGY	2 cut(s)	23, 71
PaeI	GCATGC	1 cut(s)	23
PceI	AGGCCT	1 cut(s)	358
PdiI	GCCGGC	1 cut(s)	760
PfeI	GAWTC	1 cut(s)	154
PinAI	ACCGGT	2 cut(s)	394, 485
PkrI	GCNGC	7 cut(s)	7, 127, 328, 331, 376, 403, 637
PleI	GAGTC	2 cut(s)	257, 899
PpsI	GAGTC	2 cut(s)	257, 899
PpuMI	RGGWCCY	1 cut(s)	50
Psp124BI	GAGCTC	2 cut(s)	568, 731
Psp5II	RGGWCCY	1 cut(s)	50
Psp6I	CCWGG	3 cut(s)	139, 417, 852
PspGI	CCWGG	3 cut(s)	139, 417, 852
PspN4I	GGNNCC	2 cut(s)	490, 822
PspPI	GGNCC	1 cut(s)	50
PspPPI	RGGWCCY	1 cut(s)	50
PvuII	CAGCTG	1 cut(s)	638
RsaI	GTAC	1 cut(s)	32
RsaNI	GTAC	1 cut(s)	31
SacI	GAGCTC	2 cut(s)	568, 731
SaqAI	TTAA	3 cut(s)	62, 789, 867
SatI	GCNGC	7 cut(s)	6, 126, 327, 330, 375, 402, 636
Sau3AI	GATC	4 cut(s)	441, 675, 828, 975
Sau96I	GGNCC	1 cut(s)	50
SchI	GAGTC	2 cut(s)	258, 899
ScrFI	CCNGG	3 cut(s)	141, 419, 854
SduI	GDGCHC	3 cut(s)	568, 650, 731
SfaNI	GCATC	2 cut(s)	424, 925
SinI	GGWCC	1 cut(s)	50
SmlI	CTYRAG	3 cut(s)	113, 788, 890
SmoI	CTYRAG	3 cut(s)	113, 788, 890
SpeI	ACTAGT	1 cut(s)	580
SphI	GCATGC	1 cut(s)	23
Sse9I	AATT	4 cut(s)	341, 696, 721, 910
SseBI	AGGCCT	1 cut(s)	358
SsiI	CCGC	3 cut(s)	23, 326, 552
SspMI	CTAG	3 cut(s)	354, 524, 581
SstI	GAGCTC	2 cut(s)	568, 731
StuI	AGGCCT	1 cut(s)	358
StyD4I	CCNGG	3 cut(s)	139, 417, 852
StyI	CCWWGG	1 cut(s)	701
TaaI	ACNGT	2 cut(s)	38, 898
TaiI	ACGT	1 cut(s)	504
TaqII	GACCGA	1 cut(s)	494
TasI	AATT	4 cut(s)	341, 696, 721, 910
TauI	GCSGC	1 cut(s)	329
TfiI	GAWTC	1 cut(s)	154
Tru1I	TTAA	3 cut(s)	62, 789, 867
Tru9I	TTAA	3 cut(s)	62, 789, 867
TseFI	GTSAC	4 cut(s)	250, 269, 547, 898
TseI	GCWGC	6 cut(s)	5, 125, 329, 374, 401, 635
Tsp45I	GTSAC	4 cut(s)	250, 269, 547, 898
TspDTI	ATGAA	1 cut(s)	675
Vha464I	CTTAAG	1 cut(s)	788
VpaK11BI	GGWCC	1 cut(s)	50
XapI	RAATTY	2 cut(s)	721, 910
XceI	RCATGY	2 cut(s)	23, 71
XcmI	CCANNNNNNNNNTGG	1 cut(s)	814
XspI	CTAG	3 cut(s)	354, 524, 581
Zsp2I	ATGCAT	1 cut(s)	21

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.