Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000313

Physical Location & Seq

Genomic Coordinates Reverse (-)

29855406 .. 29857998

Gene Structure 2593 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1168400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGGCAGGCATGGCGCCCAAGCTTATGCATGCGGTGCAGTACGTCCGCTATGGTGGAGGAGCTTCTGGTTTACAGCATGCAGAGGTTCCAATACCCACTCCGAAGAAAGGCGAGGTTTTGCTGAAATTGGAAGCAGCTAGTATAAACCCACTGGATTGGAAAATTCAGAAAGGCGTGGCACGACCTTTTTTGCCCAAAAAATTTCCTCATATACCTGTTACTGATGTTGCTGGAGAGGTTGTAAAAGTTGGAGAGGGCGTCCAAAAGTTCAAACCGGGCGACAAAGTTGTGGCATATCTGACCGCTGCTATTGGAGGCGGACTGGCTGAGTACGCCACAGCTAATGAGAACATGACAGCGGCCAGGCCACCAGAAGTTTCAGCAGCTGAAGCTGCAGGCTTACCTATTGCTGGCGTCACAGCTCACCAATGTCTCACGCAAGCTGCTCGGGTCAAGCTTGACGGAAGTGGCCAGCAAAAGAACATATTGATTACTGCTGCCTCTGGCGGTGTGGGTCAGTATGCAGTCCAATTAGCCAAGCTCGGTAATGCTCATGTTACGGCCACTTGTGGAGCTCGTAACGTTGAATTTGTCAAGAGCTTAGGGGCAGATGAGGTTCTTGACTACAAGACCCCAGAAGGAGCAGCTCTGAAGAGCCCATCTGGTCGGAAATATGATGCCGTGGTCCACTGCACAACCAGTACCGGTGTTCCCTGGTCAACTTTTGAGCCTAATTTGAGTTCCAACGGGAAGGTCATAGCAATCACTCCCGGTCCAAGTGCCTTTCTTACTTTTGCTCTGAAGAAACTCTCCTTCTCGAAGAAGCGGCTGGTGCCGCTGTTTGCTGATGTCAAGGCTGAGAACCTTGAGTATCTTGTTAAGTTGGTGAAGGAAGGAAAGCTCAAGACGGTAATCGACTCGACGCATCCTCTGAGCAAGGCTGAAGATGCATGGGCTAAGAGTATGGATGGACATGCAACCGGGAAGGTCATCGTTGAGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N_2

Protein Analysis

335

Amino Acids

35.0

Weight (kDa)

9.32

Isoelectric Point (pI)

22.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	36 - 121	1.2e-16	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	170 - 234	1.5e-10	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	201 - 332	7.7e-23	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13010
fragaria_vesca	FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica	MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica	Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis	pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis	RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata	RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora	Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa	Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha	Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana	Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	13, 832
AciI	CCGC	8 cut(s)	32, 46, 303, 318, 359, 507, 826, 836
AclI	AACGTT	1 cut(s)	582
AcoI	YGGCCR	3 cut(s)	360, 469, 561
AcsI	RAATTY	3 cut(s)	162, 200, 587
AcuI	CTGAAG	4 cut(s)	408, 671, 821, 963
AcyI	GRCGYC	3 cut(s)	14, 258, 414
AfaI	GTAC	3 cut(s)	41, 332, 703
AfiI	CCNNNNNNNGG	2 cut(s)	107, 410
AgeI	ACCGGT	1 cut(s)	704
AgsI	TTSAA	2 cut(s)	271, 587
AjnI	CCWGG	2 cut(s)	362, 713
Alw21I	GWGCWC	1 cut(s)	577
Alw26I	GTCTC	1 cut(s)	437
AlwNI	CAGNNNCTG	1 cut(s)	386
Ama87I	CYCGRG	1 cut(s)	447
AoxI	GGCC	4 cut(s)	360, 365, 469, 561
ApeKI	GCWGC	7 cut(s)	134, 305, 383, 392, 443, 497, 644
ApoI	RAATTY	3 cut(s)	162, 200, 587
AsiGI	ACCGGT	1 cut(s)	704
AspLEI	GCGC	1 cut(s)	16
AspS9I	GGNCC	2 cut(s)	685, 773
AsuC2I	CCSGG	3 cut(s)	276, 771, 982
AsuHPI	GGTGA	2 cut(s)	416, 898
AvaI	CYCGRG	1 cut(s)	447
AvaII	GGWCC	2 cut(s)	685, 773
BalI	TGGCCA	1 cut(s)	471
BanI	GGYRCC	2 cut(s)	13, 832
BanII	GRGCYC	2 cut(s)	577, 659
Bbv12I	GWGCWC	1 cut(s)	577
BbvI	GCAGC	7 cut(s)	146, 292, 379, 395, 430, 484, 656
BccI	CCATC	2 cut(s)	667, 962
BceAI	ACGGC	2 cut(s)	576, 665
BcgI	CGANNNNNNTGC	2 cut(s)	428, 462
BciT130I	CCWGG	2 cut(s)	364, 715
BcnI	CCSGG	3 cut(s)	276, 771, 982
BcoDI	GTCTC	1 cut(s)	437
BfaI	CTAG	1 cut(s)	138
BfmI	CTRYAG	1 cut(s)	393
BfoI	RGCGCY	1 cut(s)	17
Bme1390I	CCNGG	5 cut(s)	276, 364, 715, 771, 982
Bme18I	GGWCC	2 cut(s)	685, 773
BmeT110I	CYCGRG	1 cut(s)	447
BmgT120I	GGNCC	2 cut(s)	685, 773
BmiI	GGNNCC	3 cut(s)	15, 87, 834
BmrFI	CCNGG	5 cut(s)	276, 364, 715, 771, 982
BmsI	GCATC	3 cut(s)	667, 934, 937
BpmI	CTGGAG	1 cut(s)	252
Bpu10I	CCTNAGC	1 cut(s)	601
BpuEI	CTTGAG	2 cut(s)	887, 887
BpuMI	CCSGG	3 cut(s)	276, 771, 982
BsaHI	GRCGYC	3 cut(s)	14, 258, 414
BsaJI	CCNNGG	2 cut(s)	681, 713
BsaWI	WCCGGW	1 cut(s)	704
BsaXI	ACNNNNNCTCC	4 cut(s)	225, 243, 255, 273
Bsc4I	CCNNNNNNNGG	2 cut(s)	107, 410
Bse118I	RCCGGY	1 cut(s)	704
Bse1I	ACTGG	3 cut(s)	156, 327, 699
BseBI	CCWGG	2 cut(s)	364, 715
BseDI	CCNNGG	2 cut(s)	681, 713
BseGI	GGATG	2 cut(s)	925, 973
BseLI	CCNNNNNNNGG	2 cut(s)	107, 410
BseMII	CTCAG	3 cut(s)	318, 849, 923
BseNI	ACTGG	3 cut(s)	156, 327, 699
BseRI	GAGGAG	1 cut(s)	72
BseXI	GCAGC	7 cut(s)	146, 292, 379, 395, 430, 484, 656
BsgI	GTGCAG	2 cut(s)	56, 676
BshFI	GGCC	4 cut(s)	362, 367, 471, 563
BshNI	GGYRCC	2 cut(s)	13, 832
BshTI	ACCGGT	1 cut(s)	704
BsiHKAI	GWGCWC	1 cut(s)	577
BsiHKCI	CYCGRG	1 cut(s)	447
BsiSI	CCGG	4 cut(s)	275, 705, 771, 981
BslI	CCNNNNNNNGG	2 cut(s)	107, 410
BsmAI	GTCTC	1 cut(s)	437
BsnI	GGCC	4 cut(s)	362, 367, 471, 563
BsoBI	CYCGRG	1 cut(s)	447
Bsp1286I	GDGCHC	2 cut(s)	577, 659
BspACI	CCGC	8 cut(s)	32, 46, 303, 318, 359, 507, 826, 836
BspANI	GGCC	4 cut(s)	362, 367, 471, 563
BspCNI	CTCAG	3 cut(s)	319, 850, 924
BspLI	GGNNCC	3 cut(s)	15, 87, 834
BspMAI	CTGCAG	1 cut(s)	397
BspQI	GCTCTTC	1 cut(s)	647
BspT107I	GGYRCC	2 cut(s)	13, 832
BsrFI	RCCGGY	1 cut(s)	704
BsrI	ACTGG	3 cut(s)	156, 327, 699
BssAI	RCCGGY	1 cut(s)	704
BssECI	CCNNGG	2 cut(s)	681, 713
BssNI	GRCGYC	3 cut(s)	14, 258, 414
Bst2UI	CCWGG	2 cut(s)	364, 715
Bst4CI	ACNGT	1 cut(s)	910
Bst6I	CTCTTC	1 cut(s)	647
BstACI	GRCGYC	3 cut(s)	14, 258, 414
BstAPI	GCANNNNNTGC	1 cut(s)	34
BstC8I	GCNNGC	7 cut(s)	7, 30, 78, 397, 412, 441, 473
BstDEI	CTNAG	5 cut(s)	327, 601, 858, 932, 957
BstDSI	CCRYGG	1 cut(s)	681
BstF5I	GGATG	2 cut(s)	925, 973
BstH2I	RGCGCY	1 cut(s)	17
BstHHI	GCGC	1 cut(s)	16
BstMAI	GTCTC	1 cut(s)	437
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 34, 332, 389, 392, 832, 835, 947
BstNI	CCWGG	2 cut(s)	364, 715
BstNSI	RCATGY	3 cut(s)	32, 80, 977
BstSCI	CCNGG	5 cut(s)	274, 362, 713, 769, 980
BstSFI	CTRYAG	1 cut(s)	393
BstV1I	GCAGC	7 cut(s)	146, 292, 379, 395, 430, 484, 656
BstXI	CCANNNNNNTGG	1 cut(s)	156
BsuRI	GGCC	4 cut(s)	362, 367, 471, 563
BtgI	CCRYGG	1 cut(s)	681
BtsCI	GGATG	2 cut(s)	925, 973
BtsI	GCAGTG	1 cut(s)	688
BtsIMutI	CAGTG	2 cut(s)	149, 688
Cac8I	GCNNGC	7 cut(s)	7, 30, 78, 397, 412, 441, 473
CaiI	CAGNNNCTG	1 cut(s)	386
CfoI	GCGC	1 cut(s)	16
Cfr10I	RCCGGY	1 cut(s)	704
Cfr13I	GGNCC	2 cut(s)	685, 773
CseI	GACGC	3 cut(s)	247, 403, 931
Csp6I	GTAC	3 cut(s)	40, 331, 702
CspAI	ACCGGT	1 cut(s)	704
CviAII	CATG	7 cut(s)	10, 29, 77, 352, 554, 951, 974
CviQI	GTAC	3 cut(s)	40, 331, 702
DdeI	CTNAG	5 cut(s)	327, 601, 858, 932, 957
DinI	GGCGCC	1 cut(s)	15
EaeI	YGGCCR	3 cut(s)	360, 469, 561
Eam1104I	CTCTTC	1 cut(s)	647
EarI	CTCTTC	1 cut(s)	647
EciI	GGCGGA	1 cut(s)	333
Ecl136II	GAGCTC	1 cut(s)	575
Eco24I	GRGCYC	2 cut(s)	577, 659
Eco47I	GGWCC	2 cut(s)	685, 773
Eco53kI	GAGCTC	1 cut(s)	575
Eco57I	CTGAAG	4 cut(s)	408, 671, 821, 963
Eco88I	CYCGRG	1 cut(s)	447
EcoICRI	GAGCTC	1 cut(s)	575
EcoRII	CCWGG	2 cut(s)	362, 713
EcoT22I	ATGCAT	2 cut(s)	30, 952
EcoT38I	GRGCYC	2 cut(s)	577, 659
EgeI	GGCGCC	1 cut(s)	15
EheI	GGCGCC	1 cut(s)	15
FaeI	CATG	7 cut(s)	13, 32, 80, 355, 557, 954, 977
FatI	CATG	7 cut(s)	9, 28, 76, 351, 553, 950, 973
FokI	GGATG	2 cut(s)	912, 980
FriOI	GRGCYC	2 cut(s)	577, 659
FspBI	CTAG	1 cut(s)	138
GlaI	GCGC	1 cut(s)	15
GsuI	CTGGAG	1 cut(s)	252
HaeII	RGCGCY	1 cut(s)	17
HaeIII	GGCC	4 cut(s)	362, 367, 471, 563
HapII	CCGG	4 cut(s)	275, 705, 771, 981
HgaI	GACGC	3 cut(s)	247, 403, 931
HhaI	GCGC	1 cut(s)	16
Hin1I	GRCGYC	3 cut(s)	14, 258, 414
Hin1II	CATG	7 cut(s)	13, 32, 80, 355, 557, 954, 977
Hin6I	GCGC	1 cut(s)	14
HinP1I	GCGC	1 cut(s)	14
HincII	GTYRAC	1 cut(s)	720
HindII	GTYRAC	1 cut(s)	720
HindIII	AAGCTT	2 cut(s)	20, 455
HinfI	GANTC	1 cut(s)	917
HpaII	CCGG	4 cut(s)	275, 705, 771, 981
HphI	GGTGA	2 cut(s)	416, 898
Hpy166II	GTNNAC	3 cut(s)	71, 688, 720
Hpy188I	TCNGA	7 cut(s)	102, 168, 300, 651, 669, 801, 933
Hpy188III	TCNNGA	4 cut(s)	595, 620, 817, 904
Hpy8I	GTNNAC	3 cut(s)	71, 688, 720
Hpy99I	CGWCG	1 cut(s)	925
HpyAV	CCTTC	6 cut(s)	632, 745, 823, 883, 887, 979
HpyCH4III	ACNGT	1 cut(s)	910
HpyCH4IV	ACGT	2 cut(s)	42, 582
HpyCH4V	TGCA	8 cut(s)	28, 37, 80, 395, 524, 693, 950, 977
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 34, 332, 389, 392, 832, 835, 947
HpyF3I	CTNAG	5 cut(s)	327, 601, 858, 932, 957
HpySE526I	ACGT	2 cut(s)	42, 582
Hsp92I	GRCGYC	3 cut(s)	14, 258, 414
Hsp92II	CATG	7 cut(s)	13, 32, 80, 355, 557, 954, 977
HspAI	GCGC	1 cut(s)	14
KasI	GGCGCC	1 cut(s)	13
LguI	GCTCTTC	1 cut(s)	647
LmnI	GCTCC	3 cut(s)	59, 572, 641
Lsp1109I	GCAGC	7 cut(s)	146, 292, 379, 395, 430, 484, 656
LweI	GCATC	3 cut(s)	667, 934, 937
MaeI	CTAG	1 cut(s)	138
MaeII	ACGT	2 cut(s)	42, 582
MaeIII	GTNAC	4 cut(s)	217, 415, 556, 578
MboII	GAAGA	5 cut(s)	115, 664, 814, 832, 956
MhlI	GDGCHC	2 cut(s)	577, 659
MlsI	TGGCCA	1 cut(s)	471
MluCI	AATT	6 cut(s)	125, 162, 200, 530, 587, 733
MluNI	TGGCCA	1 cut(s)	471
Mly113I	GGCGCC	1 cut(s)	14
MlyI	GAGTC	1 cut(s)	911
MmeI	TCCRAC	3 cut(s)	229, 647, 768
Mox20I	TGGCCA	1 cut(s)	471
Mph1103I	ATGCAT	2 cut(s)	30, 952
MscI	TGGCCA	1 cut(s)	471
MseI	TTAA	2 cut(s)	879, 1003
Msp20I	TGGCCA	1 cut(s)	471
MspA1I	CMGCKG	4 cut(s)	305, 359, 386, 838
MspI	CCGG	4 cut(s)	275, 705, 771, 981
MspR9I	CCNGG	5 cut(s)	276, 364, 715, 771, 982
MvaI	CCWGG	2 cut(s)	364, 715
MwoI	GCNNNNNNNGC	8 cut(s)	11, 34, 332, 389, 392, 832, 835, 947
NarI	GGCGCC	1 cut(s)	14
NciI	CCSGG	3 cut(s)	276, 771, 982
NlaIII	CATG	7 cut(s)	13, 32, 80, 355, 557, 954, 977
NlaIV	GGNNCC	3 cut(s)	15, 87, 834
NmuCI	GTSAC	1 cut(s)	415
NsiI	ATGCAT	2 cut(s)	30, 952
NspI	RCATGY	3 cut(s)	32, 80, 977
PaeI	GCATGC	2 cut(s)	32, 80
PciSI	GCTCTTC	1 cut(s)	647
PinAI	ACCGGT	1 cut(s)	704
PleI	GAGTC	1 cut(s)	911
PluTI	GGCGCC	1 cut(s)	17
PpsI	GAGTC	1 cut(s)	911
Psp124BI	GAGCTC	1 cut(s)	577
Psp1406I	AACGTT	1 cut(s)	582
Psp6I	CCWGG	2 cut(s)	362, 713
PspGI	CCWGG	2 cut(s)	362, 713
PspN4I	GGNNCC	3 cut(s)	15, 87, 834
PspPI	GGNCC	2 cut(s)	685, 773
PstI	CTGCAG	1 cut(s)	397
PstNI	CAGNNNCTG	1 cut(s)	386
PvuII	CAGCTG	1 cut(s)	386
RsaI	GTAC	3 cut(s)	41, 332, 703
RsaNI	GTAC	3 cut(s)	40, 331, 702
SacI	GAGCTC	1 cut(s)	577
SapI	GCTCTTC	1 cut(s)	647
SaqAI	TTAA	2 cut(s)	879, 1003
Sau96I	GGNCC	2 cut(s)	685, 773
SchI	GAGTC	1 cut(s)	911
ScrFI	CCNGG	5 cut(s)	276, 364, 715, 771, 982
SduI	GDGCHC	2 cut(s)	577, 659
SfaNI	GCATC	3 cut(s)	667, 934, 937
SfcI	CTRYAG	1 cut(s)	393
SfoI	GGCGCC	1 cut(s)	15
SinI	GGWCC	2 cut(s)	685, 773
SmlI	CTYRAG	2 cut(s)	866, 902
SmoI	CTYRAG	2 cut(s)	866, 902
SphI	GCATGC	2 cut(s)	32, 80
Sse9I	AATT	6 cut(s)	125, 162, 200, 530, 587, 733
SsiI	CCGC	8 cut(s)	32, 46, 303, 318, 359, 507, 826, 836
SspDI	GGCGCC	1 cut(s)	13
SspMI	CTAG	1 cut(s)	138
SstI	GAGCTC	1 cut(s)	577
StyD4I	CCNGG	5 cut(s)	274, 362, 713, 769, 980
TaaI	ACNGT	1 cut(s)	910
TaiI	ACGT	2 cut(s)	45, 585
TaqI	TCGA	3 cut(s)	818, 915, 920
TasI	AATT	6 cut(s)	125, 162, 200, 530, 587, 733
TauI	GCSGC	3 cut(s)	362, 829, 838
Tru1I	TTAA	2 cut(s)	879, 1003
Tru9I	TTAA	2 cut(s)	879, 1003
TscAI	CASTG	2 cut(s)	156, 695
TseFI	GTSAC	1 cut(s)	415
TseI	GCWGC	7 cut(s)	134, 305, 383, 392, 443, 497, 644
Tsp45I	GTSAC	1 cut(s)	415
TspGWI	ACGGA	1 cut(s)	477
TspRI	CASTG	2 cut(s)	156, 695
VpaK11BI	GGWCC	2 cut(s)	685, 773
XapI	RAATTY	3 cut(s)	162, 200, 587
XceI	RCATGY	3 cut(s)	32, 80, 977
XspI	CTAG	1 cut(s)	138
Zsp2I	ATGCAT	2 cut(s)	30, 952

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.