Rh6AG268700

Zinc-binding dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000313
Physical Location & Seq
Forward (+)
46172443 .. 46178125
5683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG268700.1

Sequence Viewer

Length: 861 bp
ATGGCGGCAAAACTTATGCGTGCGGTTCAGTACGACGGATATGGTGGAGGACCTTCCGGTCTAAAGCATGTTGAGGTTCCGATCCCAACTCCGAAGAAAGATGAGGTTTTGCTGAAATTGGAAGCGGCTACCATAAATCCAATTGATTGGAAAGTTCAGAAAGGCGTACTGCGCCCTTTTTTACCAAGAAAGTTCCCTTATATACCTGTTAGTGATGTGGCTGGAGAGGTTGTCGACGTTGGACAAGGAGTTCAGAAGTTCAAAGCGGGCGACAAAGTTGTAGCATTTTTCACCCAGGCTAATGGAGGTGGATTTGCTGAGTTTGCAACTGCTAGTGAGAGTTTGACCGTTACTAGGCCGCCTGAAGTTTCAGCAGCCGAAGGTGCAGGGTTACCTATTGCTGGTCTCACAGCTCACCAGTGTCTCACCCAAGCTGCAGGAGTCAAGCTTGATGGCACTGGCCCACAGAAGAACATATTGGTTACTGCTGCCTCAGGCGGTGTAGGTCACTATGCAGTTCAATTGGCAAAGCTTGGGAACACTCACGTGACAGCCACTTGTGGTGCACGCAACGTTGAATTTGTCAAGAGCTTAGGGGCCGATGAGGTTCTTGACTACAAGACCCCTGAAGGGGCAGCTCTGAATAGCCCTTCTGGTCGGAAGTACGATGCTGTTATCCACTGTGCAACTGGCATTCCTTGGTCCACATTTGAGCCGAATTTGAGTCCAAATGGGAAGGTTATAGACATTACTCCCGGTCCAAGTGCATTGTGTACTTTCGCTCTGAAGAAACTCGATCACCTTCTCCAAGAAGCAACTGGTGCCGCTGCTCATGAATGCCAAGGCGGAGAATCTGGATGA

Protein Analysis

286

Amino Acids

29.64

Weight (kDa)

6.65

Isoelectric Point (pI)

26.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 33 - 118 2.3e-15 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 167 - 242 1.4e-09 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13010
fragaria_vesca FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 821
AccI GTMKAC 1 cut(s) 234
AciI CCGC 8 cut(s) 5, 23, 125, 266, 359, 498, 825, 846
AclI AACGTT 1 cut(s) 573
AclWI GGATC 1 cut(s) 76
AcsI RAATTY 2 cut(s) 578, 718
AcuI CTGAAG 3 cut(s) 384, 648, 806
AcvI CACGTG 1 cut(s) 547
AfaI GTAC 4 cut(s) 32, 168, 665, 775
AfiI CCNNNNNNNGG 4 cut(s) 354, 401, 630, 631
AgsI TTSAA 3 cut(s) 262, 521, 578
AjnI CCWGG 1 cut(s) 294
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AleI CACNNNNGTG 1 cut(s) 545
AloI GAACNNNNNNTCC 2 cut(s) 234, 266
AluBI AGCT 6 cut(s) 413, 434, 448, 532, 591, 638
AluI AGCT 6 cut(s) 413, 434, 448, 532, 591, 638
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 410, 428
Alw44I GTGCAC 1 cut(s) 564
AlwI GGATC 1 cut(s) 76
AoxI GGCC 3 cut(s) 356, 460, 597
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 5 cut(s) 374, 434, 488, 635, 827
ApoI RAATTY 2 cut(s) 578, 718
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 5 cut(s) 50, 461, 597, 702, 758
AsuC2I CCSGG 1 cut(s) 756
AsuHPI GGTGA 4 cut(s) 283, 407, 418, 791
AvaII GGWCC 3 cut(s) 50, 702, 758
AxyI CCTNAGG 1 cut(s) 493
BaeGI GKGCMC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 821
BbrPI CACGTG 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 568
BbvI GCAGC 5 cut(s) 386, 421, 475, 647, 814
BccI CCATC 1 cut(s) 446
BciT130I CCWGG 1 cut(s) 296
BcnI CCSGG 1 cut(s) 756
BcoDI GTCTC 2 cut(s) 410, 428
BfaI CTAG 2 cut(s) 333, 354
BfmI CTRYAG 1 cut(s) 435
BisI GCNGC 9 cut(s) 6, 126, 359, 375, 435, 489, 636, 825, 828
BlsI GCNGC 9 cut(s) 7, 127, 360, 376, 436, 490, 637, 826, 829
Bme1390I CCNGG 2 cut(s) 296, 756
Bme18I GGWCC 3 cut(s) 50, 702, 758
BmgT120I GGNCC 5 cut(s) 50, 461, 597, 702, 758
BmiI GGNNCC 3 cut(s) 78, 598, 823
BmrFI CCNGG 2 cut(s) 296, 756
BmsI GCATC 1 cut(s) 658
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 592
BpuMI CCSGG 1 cut(s) 756
BsaAI YACGTR 1 cut(s) 547
BsaI GGTCTC 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 294, 698, 841
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 4 cut(s) 354, 401, 630, 631
Bse1I ACTGG 4 cut(s) 418, 463, 694, 823
Bse21I CCTNAGG 1 cut(s) 493
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 294, 698, 841
BseLI CCNNNNNNNGG 4 cut(s) 354, 401, 630, 631
BseMII CTCAG 2 cut(s) 309, 507
BseNI ACTGG 4 cut(s) 418, 463, 694, 823
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 5 cut(s) 386, 421, 475, 647, 814
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 3 cut(s) 358, 462, 599
BshNI GGYRCC 1 cut(s) 821
BsiHKAI GWGCWC 1 cut(s) 568
BsiSI CCGG 2 cut(s) 57, 756
BslI CCNNNNNNNGG 4 cut(s) 354, 401, 630, 631
BsmAI GTCTC 2 cut(s) 410, 428
BsmI GAATGC 2 cut(s) 693, 842
BsnI GGCC 3 cut(s) 358, 462, 599
Bso31I GGTCTC 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 568
Bsp143I GATC 2 cut(s) 81, 796
BspACI CCGC 8 cut(s) 5, 23, 125, 266, 359, 498, 825, 846
BspANI GGCC 3 cut(s) 358, 462, 599
BspCNI CTCAG 2 cut(s) 310, 506
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 3 cut(s) 78, 598, 823
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 1 cut(s) 76
BspT107I GGYRCC 1 cut(s) 821
BspTNI GGTCTC 1 cut(s) 410
BsrI ACTGG 4 cut(s) 418, 463, 694, 823
BssECI CCNNGG 3 cut(s) 294, 698, 841
BssMI GATC 2 cut(s) 81, 796
BssT1I CCWWGG 2 cut(s) 698, 841
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 2 cut(s) 349, 683
BstAPI GCANNNNNTGC 1 cut(s) 821
BstBAI YACGTR 1 cut(s) 547
BstC8I GCNNGC 3 cut(s) 21, 268, 568
BstDEI CTNAG 3 cut(s) 318, 493, 592
BstEII GGTNACC 1 cut(s) 390
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 2 cut(s) 84, 799
BstMAI GTCTC 2 cut(s) 410, 428
BstMBI GATC 2 cut(s) 81, 796
BstMWI GCNNNNNNNGC 4 cut(s) 171, 323, 383, 821
BstNI CCWGG 1 cut(s) 296
BstNSI RCATGY 1 cut(s) 71
BstPI GGTNACC 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 294, 754
BstSFI CTRYAG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 568
BstV1I GCAGC 5 cut(s) 386, 421, 475, 647, 814
BstXI CCANNNNNNTGG 2 cut(s) 147, 302
Bsu36I CCTNAGG 1 cut(s) 493
BsuRI GGCC 3 cut(s) 358, 462, 599
BtsIMutI CAGTG 3 cut(s) 425, 456, 679
Cac8I GCNNGC 3 cut(s) 21, 268, 568
CciI TCATGA 1 cut(s) 832
CfoI GCGC 1 cut(s) 174
Cfr13I GGNCC 5 cut(s) 50, 461, 597, 702, 758
Csp6I GTAC 4 cut(s) 31, 167, 664, 774
CviAII CATG 2 cut(s) 68, 833
CviQI GTAC 4 cut(s) 31, 167, 664, 774
DdeI CTNAG 3 cut(s) 318, 493, 592
DpnI GATC 2 cut(s) 83, 798
DpnII GATC 2 cut(s) 81, 796
DrdI GACNNNNNNGTC 1 cut(s) 57
DseDI GACNNNNNNGTC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 861
Eco130I CCWWGG 2 cut(s) 698, 841
Eco31I GGTCTC 1 cut(s) 410
Eco47I GGWCC 3 cut(s) 50, 702, 758
Eco57I CTGAAG 3 cut(s) 384, 648, 806
Eco72I CACGTG 1 cut(s) 547
Eco81I CCTNAGG 1 cut(s) 493
Eco91I GGTNACC 1 cut(s) 390
EcoO109I RGGNCCY 1 cut(s) 50
EcoO65I GGTNACC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 2 cut(s) 698, 841
ErhI CCWWGG 2 cut(s) 698, 841
FaeI CATG 2 cut(s) 71, 836
FatI CATG 2 cut(s) 67, 832
FauI CCCGC 1 cut(s) 259
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 9 cut(s) 6, 126, 359, 375, 435, 489, 636, 825, 828
Fsp4HI GCNGC 9 cut(s) 6, 126, 359, 375, 435, 489, 636, 825, 828
FspBI CTAG 2 cut(s) 333, 354
GlaI GCGC 1 cut(s) 173
GluI GCNGC 9 cut(s) 6, 126, 359, 375, 435, 489, 636, 825, 828
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 3 cut(s) 358, 462, 599
HapII CCGG 2 cut(s) 57, 756
HhaI GCGC 1 cut(s) 174
Hin1II CATG 2 cut(s) 71, 836
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 2 cut(s) 446, 530
HinfI GANTC 3 cut(s) 441, 724, 851
HpaII CCGG 2 cut(s) 57, 756
HphI GGTGA 4 cut(s) 283, 407, 418, 791
Hpy166II GTNNAC 4 cut(s) 235, 566, 705, 774
Hpy188I TCNGA 7 cut(s) 81, 93, 159, 255, 642, 660, 786
Hpy188III TCNNGA 4 cut(s) 586, 611, 833, 855
Hpy8I GTNNAC 4 cut(s) 235, 566, 705, 774
Hpy99I CGWCG 2 cut(s) 38, 239
HpyAV CCTTC 6 cut(s) 63, 374, 623, 660, 730, 812
HpyCH4III ACNGT 2 cut(s) 349, 683
HpyCH4IV ACGT 3 cut(s) 237, 546, 573
HpyCH4V TGCA 7 cut(s) 326, 386, 437, 515, 566, 686, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 323, 383, 821
HpyF3I CTNAG 3 cut(s) 318, 493, 592
HpySE526I ACGT 3 cut(s) 237, 546, 573
Hsp92II CATG 2 cut(s) 71, 836
HspAI GCGC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 81, 796
Lsp1109I GCAGC 5 cut(s) 386, 421, 475, 647, 814
LweI GCATC 1 cut(s) 658
MaeI CTAG 2 cut(s) 333, 354
MaeII ACGT 3 cut(s) 237, 546, 573
MaeIII GTNAC 5 cut(s) 349, 390, 481, 506, 547
MalI GATC 2 cut(s) 83, 798
MboI GATC 2 cut(s) 81, 796
MboII GAAGA 3 cut(s) 106, 481, 799
MfeI CAATTG 2 cut(s) 141, 521
MhlI GDGCHC 1 cut(s) 568
MluCI AATT 5 cut(s) 116, 141, 521, 578, 718
MlyI GAGTC 2 cut(s) 450, 733
MmeI TCCRAC 2 cut(s) 220, 638
MnlI CCTC 7 cut(s) 41, 67, 97, 220, 299, 502, 598
MslI CAYNNNNRTG 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 827
MspI CCGG 2 cut(s) 57, 756
MspR9I CCNGG 2 cut(s) 296, 756
MunI CAATTG 2 cut(s) 141, 521
Mva1269I GAATGC 2 cut(s) 693, 842
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 4 cut(s) 171, 323, 383, 821
NciI CCSGG 1 cut(s) 756
NdeII GATC 2 cut(s) 81, 796
NlaIII CATG 2 cut(s) 71, 836
NlaIV GGNNCC 3 cut(s) 78, 598, 823
NmuCI GTSAC 2 cut(s) 506, 547
NspI RCATGY 1 cut(s) 71
OliI CACNNNNGTG 1 cut(s) 545
PagI TCATGA 1 cut(s) 832
PctI GAATGC 2 cut(s) 693, 842
PfeI GAWTC 1 cut(s) 851
PkrI GCNGC 9 cut(s) 7, 127, 360, 376, 436, 490, 637, 826, 829
PleI GAGTC 2 cut(s) 449, 732
PmaCI CACGTG 1 cut(s) 547
PmlI CACGTG 1 cut(s) 547
PpsI GAGTC 2 cut(s) 449, 732
Ppu21I YACGTR 1 cut(s) 547
PpuMI RGGWCCY 1 cut(s) 50
Psp1406I AACGTT 1 cut(s) 573
Psp5II RGGWCCY 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 294
PspCI CACGTG 1 cut(s) 547
PspEI GGTNACC 1 cut(s) 390
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 78, 598, 823
PspPI GGNCC 5 cut(s) 50, 461, 597, 702, 758
PspPPI RGGWCCY 1 cut(s) 50
PstI CTGCAG 1 cut(s) 439
RsaI GTAC 4 cut(s) 32, 168, 665, 775
RsaNI GTAC 4 cut(s) 31, 167, 664, 774
RseI CAYNNNNRTG 1 cut(s) 545
SalI GTCGAC 1 cut(s) 233
SatI GCNGC 9 cut(s) 6, 126, 359, 375, 435, 489, 636, 825, 828
Sau3AI GATC 2 cut(s) 81, 796
Sau96I GGNCC 5 cut(s) 50, 461, 597, 702, 758
SchI GAGTC 2 cut(s) 450, 733
ScrFI CCNGG 2 cut(s) 296, 756
SduI GDGCHC 1 cut(s) 568
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 3 cut(s) 50, 702, 758
SmiMI CAYNNNNRTG 1 cut(s) 545
Sse9I AATT 5 cut(s) 116, 141, 521, 578, 718
SsiI CCGC 8 cut(s) 5, 23, 125, 266, 359, 498, 825, 846
SspMI CTAG 2 cut(s) 333, 354
StyD4I CCNGG 2 cut(s) 294, 754
StyI CCWWGG 2 cut(s) 698, 841
TaaI ACNGT 2 cut(s) 349, 683
TaiI ACGT 3 cut(s) 240, 549, 576
TaqI TCGA 2 cut(s) 234, 795
TasI AATT 5 cut(s) 116, 141, 521, 578, 718
TatI WGTACW 1 cut(s) 773
TauI GCSGC 4 cut(s) 8, 128, 361, 827
TfiI GAWTC 1 cut(s) 851
TscAI CASTG 3 cut(s) 425, 463, 686
TseFI GTSAC 2 cut(s) 506, 547
TseI GCWGC 5 cut(s) 374, 434, 488, 635, 827
Tsp45I GTSAC 2 cut(s) 506, 547
TspDTI ATGAA 1 cut(s) 849
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 3 cut(s) 425, 463, 686
VneI GTGCAC 1 cut(s) 564
VpaK11BI GGWCC 3 cut(s) 50, 702, 758
XapI RAATTY 2 cut(s) 578, 718
XceI RCATGY 1 cut(s) 71
XcmI CCANNNNNNNNNTGG 2 cut(s) 686, 815
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 2 cut(s) 333, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.