Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000313

Physical Location & Seq

Genomic Coordinates Reverse (-)

29860406 .. 29862383

Gene Structure 1978 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1168600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 996 bp

ATGGCAGCAATGGCGTCGAAGCTTATGCATGCGGTTCAGTACGACGGCTATGGCGGAGGAGCTTCTGGTTTAAAGCATGTAGAGGTTCCGATCCCCCCTCCGACGAAAGGCGAGGTTTTGCTGAAACTGGAAGCAGCCAGTATAAACCCAATTGATTGGAAAATTCAGAAAGGCATGTTGCGGCCTTTTTTGCCCCGCAAATTCCCTTATACACCCGTTACTGATGTTGCTGGAGAGGTTGTAGAAGTTGGACAGGGGGTCCAAAAGTTCAAACCTGGCGACAAAGTTGTGGCATATCTTTCCCAAGCTGGAGGTGGACTGGCCAAGTACGCTGCAGCTACTGAGAACTTGACAGTGACCAGGCCACCTGAAGTATCAGCAGCTGAAGGTGCAGGCTTACCTGTTGCTGGCCTCACAGCTCACCAGTGTCTCACGCAGGCTGCTGGGGTCAAGCTGGACGGAAGCGGCCAGCAAAAGAACATACTGATTACTGCTGCCTCTGGTGGTGTGGGTCAATATGCAGTCCAACTAGCAAAGCTTGGAAACACGCATGTTACAGCTACTTGTGGAGCTCGGAACATTGAATTTGTCAAGAGCTTAGGGGCAGATGAGGTTCTTGACTACAAGACCCCAGAAGGAGCAGCTCTGAATAGCCCATCTGGTCGGAAATATGATGCCGTGGTCCACTGTGCAACCGGCATTCCCTGGTCAACTTTTGAGCCTAATTTGAGCGCCAACGGGAAGGTGATAGACATTACTCCCGGTCCAAGTGCCTTGGTTACTTTTGCTCTGAAGAAACTCACCTTCTCAAAGAAGCAGCTGGTGCCGCTGCTCATGAATGCCAAGGCTGAGAACCTGGAGTATCTCGTTAAGTTGGTGAAGGAAGGAAAGCTCAGGACAGTGATTGATTCGACATATCCTCTGAGCAAGGCTGAAGATGCTTGGGCTAACAGTATCAGTGGACATGCGACCGGGAAGATCATCGTCGAGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N_2

Protein Analysis

332

Amino Acids

34.67

Weight (kDa)

9.01

Isoelectric Point (pI)

26.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	36 - 119	9.4e-16	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	169 - 293	4.5e-10	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	200 - 329	1.2e-23	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13010
fragaria_vesca	FvH4_2g18240 FvH4_2g18280 FvH4_2g18310 FvH4_2g18320 FvH4_2g18330 FvH4_2g18340 FvH4_2g18350
malus_domestica	MD05G1166800.v1.1 MD05G1167200.v1.1 MD05G1167300.v1.1 MD05G1167400.v1.1 MD05G1167600.v1.1 MD05G1168000.v1.1 MD05G1168200.v1.1 MD05G1168400.v1.1 MD05G1168600.v1.1 MD05G1168700.v1.1 MD05G1168800.v1.1 MD10G1156900.v1.1 MD10G1157000.v1.1 MD10G1157100.v1.1 MD10G1157200.v1.1
prunus_persica	Prupe.8G195100_v2.0.a1 Prupe.8G195100_v2.0.a1 Prupe.8G195300_v2.0.a1 Prupe.8G195600_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195700_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195800_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G195900_v2.0.a1 Prupe.8G196000_v2.0.a1 Prupe.8G196000_v2.0.a1
pyrus_communis	pycom05g15560 pycom05g15600 pycom05g15610 pycom05g15620 pycom05g15630 pycom10g13550 pycom10g13570
rosa_chinensis	RchiOBHm_Chr6g0283611 RchiOBHm_Chr6g0283631 RchiOBHm_Chr6g0283641 RchiOBHm_Chr6g0283661 RchiOBHm_Chr6g0283681 RchiOBHm_Chr6g0283691 RchiOBHm_Chr6g0283701 RchiOBHm_Chr6g0283711 RchiOBHm_Chr6g0283721
rosa_laevigata	RLG00000012804 RLG00000012807 RLG00000012811
rosa_multiflora	Rmu_co8263921.1_g000001 Rmu_sc0001521.1_g000009 Rmu_sc0002906.1_g000002 Rmu_sc0002906.1_g000011 Rmu_sc0002906.1_g000013 Rmu_sc0005719.1_g000016 Rmu_sc0005719.1_g000022 Rmu_sc0029460.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185240 Rroxscaffold_7G00185250 Rroxscaffold_7G00185270 Rroxscaffold_7G00185280 Rroxscaffold_7G00185290 Rroxscaffold_7G00185330 Rroxscaffold_7G00185350
rosa_rugosa	Rorug06G0156100 Rorug06G0156300 Rorug06G0156400 Rorug06G0156500 Rorug06G0156600 Rorug06G0156700 Rorug06G0156800 Rorug06G0156900 Rorug06G0157000
rosa_samantha	Rh6AG267900 Rh6AG268100 Rh6AG268200 Rh6AG268400 Rh6AG268600 Rh6AG268700 Rh6AG268900 Rh6AG269100 Rh6BG270700 Rh6BG270900 Rh6BG271000 Rh6BG271200 Rh6BG271400 Rh6BG271500 Rh6CG270200 Rh6CG270400 Rh6CG270500 Rh6CG270800 Rh6CG270900 Rh6CG271000 Rh6CG271100 Rh6CG271300 Rh6DG263900 Rh6DG264100 Rh6DG264200 Rh6DG264400 Rh6DG264600 Rh6DG264800 Rh6DG265200
rosa_wichuraiana	Rw6G023210 Rw6G023220 Rw6G023230 Rw6G023240 Rw6G023250 Rw6G023260 Rw6G023270 Rw6G023280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	823
AciI	CCGC	6 cut(s)	32, 54, 181, 196, 465, 827
AclWI	GGATC	1 cut(s)	85
AcoI	YGGCCR	2 cut(s)	321, 466
AcsI	RAATTY	3 cut(s)	162, 200, 584
AcuI	CTGAAG	4 cut(s)	390, 405, 812, 954
AcyI	GRCGYC	1 cut(s)	14
AfaI	GTAC	2 cut(s)	41, 329
AfiI	CCNNNNNNNGG	2 cut(s)	107, 407
AgsI	TTSAA	2 cut(s)	271, 584
AhdI	GACNNNNNGTC	1 cut(s)	257
AjnI	CCWGG	4 cut(s)	274, 359, 704, 855
Alw21I	GWGCWC	1 cut(s)	574
Alw26I	GTCTC	1 cut(s)	434
AlwI	GGATC	1 cut(s)	85
AlwNI	CAGNNNCTG	2 cut(s)	341, 383
AoxI	GGCC	5 cut(s)	182, 321, 362, 409, 466
ApoI	RAATTY	3 cut(s)	162, 200, 584
AspLEI	GCGC	1 cut(s)	734
AspS9I	GGNCC	3 cut(s)	259, 682, 764
AsuC2I	CCSGG	2 cut(s)	762, 973
AsuHPI	GGTGA	4 cut(s)	413, 757, 793, 889
AvaII	GGWCC	3 cut(s)	259, 682, 764
BalI	TGGCCA	1 cut(s)	323
BanI	GGYRCC	1 cut(s)	823
BanII	GRGCYC	1 cut(s)	574
Bbv12I	GWGCWC	1 cut(s)	574
BccI	CCATC	1 cut(s)	664
BceAI	ACGGC	2 cut(s)	61, 662
BcgI	CGANNNNNNTGC	3 cut(s)	7, 31, 41
BciT130I	CCWGG	4 cut(s)	276, 361, 706, 857
BcnI	CCSGG	2 cut(s)	762, 973
BcoDI	GTCTC	1 cut(s)	434
BfaI	CTAG	1 cut(s)	530
BfmI	CTRYAG	1 cut(s)	333
BfoI	RGCGCY	1 cut(s)	735
Bme1390I	CCNGG	6 cut(s)	276, 361, 706, 762, 857, 973
Bme18I	GGWCC	3 cut(s)	259, 682, 764
BmeRI	GACNNNNNGTC	1 cut(s)	257
BmgT120I	GGNCC	3 cut(s)	259, 682, 764
BmiI	GGNNCC	3 cut(s)	87, 260, 825
BmrFI	CCNGG	6 cut(s)	276, 361, 706, 762, 857, 973
BmsI	GCATC	2 cut(s)	664, 928
BpmI	CTGGAG	3 cut(s)	252, 330, 878
Bpu10I	CCTNAGC	2 cut(s)	598, 893
BpuMI	CCSGG	2 cut(s)	762, 973
BsaHI	GRCGYC	1 cut(s)	14
BsaJI	CCNNGG	4 cut(s)	678, 704, 774, 843
BsaXI	ACNNNNNCTCC	2 cut(s)	225, 255
Bsc4I	CCNNNNNNNGG	2 cut(s)	107, 407
Bse118I	RCCGGY	1 cut(s)	695
Bse1I	ACTGG	4 cut(s)	132, 138, 324, 424
Bse3DI	GCAATG	1 cut(s)	15
BseBI	CCWGG	4 cut(s)	276, 361, 706, 857
BseDI	CCNNGG	4 cut(s)	678, 704, 774, 843
BseLI	CCNNNNNNNGG	2 cut(s)	107, 407
BseMI	GCAATG	1 cut(s)	15
BseMII	CTCAG	4 cut(s)	333, 840, 907, 914
BseNI	ACTGG	4 cut(s)	132, 138, 324, 424
BseRI	GAGGAG	1 cut(s)	72
BseYI	CCCAGC	1 cut(s)	443
BsgI	GTGCAG	1 cut(s)	411
Bsh1285I	CGRYCG	1 cut(s)	972
BshFI	GGCC	5 cut(s)	184, 323, 364, 411, 468
BshNI	GGYRCC	1 cut(s)	823
BsiEI	CGRYCG	1 cut(s)	972
BsiHKAI	GWGCWC	1 cut(s)	574
BsiSI	CCGG	3 cut(s)	696, 762, 972
BslI	CCNNNNNNNGG	2 cut(s)	107, 407
BsmAI	GTCTC	1 cut(s)	434
BsmI	GAATGC	2 cut(s)	699, 844
BsnI	GGCC	5 cut(s)	184, 323, 364, 411, 468
Bsp1286I	GDGCHC	1 cut(s)	574
Bsp143I	GATC	2 cut(s)	90, 978
BspACI	CCGC	6 cut(s)	32, 54, 181, 196, 465, 827
BspANI	GGCC	5 cut(s)	184, 323, 364, 411, 468
BspCNI	CTCAG	4 cut(s)	334, 841, 906, 915
BspHI	TCATGA	1 cut(s)	834
BspLI	GGNNCC	3 cut(s)	87, 260, 825
BspMAI	CTGCAG	1 cut(s)	337
BspPI	GGATC	1 cut(s)	85
BspT107I	GGYRCC	1 cut(s)	823
BsrDI	GCAATG	1 cut(s)	15
BsrFI	RCCGGY	1 cut(s)	695
BsrI	ACTGG	4 cut(s)	132, 138, 324, 424
BssAI	RCCGGY	1 cut(s)	695
BssECI	CCNNGG	4 cut(s)	678, 704, 774, 843
BssMI	GATC	2 cut(s)	90, 978
BssNI	GRCGYC	1 cut(s)	14
BssT1I	CCWWGG	2 cut(s)	774, 843
Bst2UI	CCWGG	4 cut(s)	276, 361, 706, 857
Bst4CI	ACNGT	4 cut(s)	355, 689, 901, 953
BstACI	GRCGYC	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	823
BstC8I	GCNNGC	5 cut(s)	30, 394, 409, 438, 470
BstDEI	CTNAG	5 cut(s)	342, 598, 849, 893, 923
BstDSI	CCRYGG	1 cut(s)	678
BstH2I	RGCGCY	1 cut(s)	735
BstHHI	GCGC	1 cut(s)	734
BstKTI	GATC	2 cut(s)	93, 981
BstMAI	GTCTC	1 cut(s)	434
BstMBI	GATC	2 cut(s)	90, 978
BstMCI	CGRYCG	1 cut(s)	972
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 190, 329, 389, 823, 826, 938
BstNI	CCWGG	4 cut(s)	276, 361, 706, 857
BstNSI	RCATGY	5 cut(s)	32, 80, 178, 554, 968
BstSCI	CCNGG	6 cut(s)	274, 359, 704, 760, 855, 971
BstSFI	CTRYAG	1 cut(s)	333
BstXI	CCANNNNNNTGG	1 cut(s)	156
BsuRI	GGCC	5 cut(s)	184, 323, 364, 411, 468
BtgI	CCRYGG	1 cut(s)	678
BtsIMutI	CAGTG	5 cut(s)	360, 431, 685, 906, 964
Cac8I	GCNNGC	5 cut(s)	30, 394, 409, 438, 470
CaiI	CAGNNNCTG	2 cut(s)	341, 383
CciI	TCATGA	1 cut(s)	834
CfoI	GCGC	1 cut(s)	734
Cfr10I	RCCGGY	1 cut(s)	695
Cfr13I	GGNCC	3 cut(s)	259, 682, 764
CseI	GACGC	1 cut(s)	3
Csp6I	GTAC	2 cut(s)	40, 328
CviAII	CATG	6 cut(s)	29, 77, 175, 551, 835, 965
CviQI	GTAC	2 cut(s)	40, 328
DdeI	CTNAG	5 cut(s)	342, 598, 849, 893, 923
DpnI	GATC	2 cut(s)	92, 980
DpnII	GATC	2 cut(s)	90, 978
DraI	TTTAAA	1 cut(s)	72
DriI	GACNNNNNGTC	1 cut(s)	257
EaeI	YGGCCR	2 cut(s)	321, 466
Eam1105I	GACNNNNNGTC	1 cut(s)	257
EciI	GGCGGA	1 cut(s)	69
Ecl136II	GAGCTC	1 cut(s)	572
Eco130I	CCWWGG	2 cut(s)	774, 843
Eco24I	GRGCYC	1 cut(s)	574
Eco47I	GGWCC	3 cut(s)	259, 682, 764
Eco53kI	GAGCTC	1 cut(s)	572
Eco57I	CTGAAG	4 cut(s)	390, 405, 812, 954
EcoICRI	GAGCTC	1 cut(s)	572
EcoRII	CCWGG	4 cut(s)	274, 359, 704, 855
EcoT14I	CCWWGG	2 cut(s)	774, 843
EcoT22I	ATGCAT	1 cut(s)	30
EcoT38I	GRGCYC	1 cut(s)	574
ErhI	CCWWGG	2 cut(s)	774, 843
FaeI	CATG	6 cut(s)	32, 80, 178, 554, 838, 968
FatI	CATG	6 cut(s)	28, 76, 174, 550, 834, 964
FauI	CCCGC	1 cut(s)	203
FriOI	GRGCYC	1 cut(s)	574
FspBI	CTAG	1 cut(s)	530
GlaI	GCGC	1 cut(s)	733
GsaI	CCCAGC	1 cut(s)	447
GsuI	CTGGAG	3 cut(s)	252, 330, 878
HaeII	RGCGCY	1 cut(s)	735
HaeIII	GGCC	5 cut(s)	184, 323, 364, 411, 468
HapII	CCGG	3 cut(s)	696, 762, 972
HgaI	GACGC	1 cut(s)	3
HhaI	GCGC	1 cut(s)	734
Hin1I	GRCGYC	1 cut(s)	14
Hin1II	CATG	6 cut(s)	32, 80, 178, 554, 838, 968
Hin6I	GCGC	1 cut(s)	732
HinP1I	GCGC	1 cut(s)	732
HincII	GTYRAC	1 cut(s)	711
HindII	GTYRAC	1 cut(s)	711
HindIII	AAGCTT	2 cut(s)	20, 536
HinfI	GANTC	1 cut(s)	908
HpaII	CCGG	3 cut(s)	696, 762, 972
HphI	GGTGA	4 cut(s)	413, 757, 793, 889
Hpy166II	GTNNAC	4 cut(s)	317, 685, 711, 962
Hpy188I	TCNGA	8 cut(s)	90, 102, 168, 576, 648, 666, 792, 924
Hpy188III	TCNNGA	4 cut(s)	592, 617, 835, 895
Hpy8I	GTNNAC	4 cut(s)	317, 685, 711, 962
Hpy99I	CGWCG	4 cut(s)	19, 47, 106, 989
HpyAV	CCTTC	6 cut(s)	380, 629, 736, 814, 874, 878
HpyCH4III	ACNGT	4 cut(s)	355, 689, 901, 953
HpyCH4V	TGCA	5 cut(s)	28, 335, 392, 521, 692
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 190, 329, 389, 823, 826, 938
HpyF3I	CTNAG	5 cut(s)	342, 598, 849, 893, 923
Hsp92I	GRCGYC	1 cut(s)	14
Hsp92II	CATG	6 cut(s)	32, 80, 178, 554, 838, 968
HspAI	GCGC	1 cut(s)	732
Kzo9I	GATC	2 cut(s)	90, 978
LmnI	GCTCC	3 cut(s)	59, 569, 638
LweI	GCATC	2 cut(s)	664, 928
MaeI	CTAG	1 cut(s)	530
MaeIII	GTNAC	4 cut(s)	217, 355, 553, 778
MalI	GATC	2 cut(s)	92, 980
MboI	GATC	2 cut(s)	90, 978
MboII	GAAGA	3 cut(s)	805, 947, 988
MfeI	CAATTG	1 cut(s)	150
MhlI	GDGCHC	1 cut(s)	574
MlsI	TGGCCA	1 cut(s)	323
MluCI	AATT	5 cut(s)	150, 162, 200, 584, 724
MluNI	TGGCCA	1 cut(s)	323
MmeI	TCCRAC	4 cut(s)	125, 229, 550, 644
Mox20I	TGGCCA	1 cut(s)	323
Mph1103I	ATGCAT	1 cut(s)	30
MscI	TGGCCA	1 cut(s)	323
MseI	TTAA	3 cut(s)	71, 870, 994
Msp20I	TGGCCA	1 cut(s)	323
MspA1I	CMGCKG	3 cut(s)	383, 820, 829
MspI	CCGG	3 cut(s)	696, 762, 972
MspR9I	CCNGG	6 cut(s)	276, 361, 706, 762, 857, 973
MunI	CAATTG	1 cut(s)	150
Mva1269I	GAATGC	2 cut(s)	699, 844
MvaI	CCWGG	4 cut(s)	276, 361, 706, 857
MwoI	GCNNNNNNNGC	7 cut(s)	11, 190, 329, 389, 823, 826, 938
NciI	CCSGG	2 cut(s)	762, 973
NdeII	GATC	2 cut(s)	90, 978
NlaIII	CATG	6 cut(s)	32, 80, 178, 554, 838, 968
NlaIV	GGNNCC	3 cut(s)	87, 260, 825
NmuCI	GTSAC	1 cut(s)	355
NsiI	ATGCAT	1 cut(s)	30
NspI	RCATGY	5 cut(s)	32, 80, 178, 554, 968
PaeI	GCATGC	1 cut(s)	32
PagI	TCATGA	1 cut(s)	834
PctI	GAATGC	2 cut(s)	699, 844
PfeI	GAWTC	1 cut(s)	908
Psp124BI	GAGCTC	1 cut(s)	574
Psp6I	CCWGG	4 cut(s)	274, 359, 704, 855
PspFI	CCCAGC	1 cut(s)	443
PspGI	CCWGG	4 cut(s)	274, 359, 704, 855
PspN4I	GGNNCC	3 cut(s)	87, 260, 825
PspPI	GGNCC	3 cut(s)	259, 682, 764
PstI	CTGCAG	1 cut(s)	337
PstNI	CAGNNNCTG	2 cut(s)	341, 383
PvuII	CAGCTG	2 cut(s)	383, 820
RsaI	GTAC	2 cut(s)	41, 329
RsaNI	GTAC	2 cut(s)	40, 328
SacI	GAGCTC	1 cut(s)	574
SaqAI	TTAA	3 cut(s)	71, 870, 994
Sau3AI	GATC	2 cut(s)	90, 978
Sau96I	GGNCC	3 cut(s)	259, 682, 764
ScrFI	CCNGG	6 cut(s)	276, 361, 706, 762, 857, 973
SduI	GDGCHC	1 cut(s)	574
SfaNI	GCATC	2 cut(s)	664, 928
SfcI	CTRYAG	1 cut(s)	333
SinI	GGWCC	3 cut(s)	259, 682, 764
SphI	GCATGC	1 cut(s)	32
Sse9I	AATT	5 cut(s)	150, 162, 200, 584, 724
SsiI	CCGC	6 cut(s)	32, 54, 181, 196, 465, 827
SspMI	CTAG	1 cut(s)	530
SstI	GAGCTC	1 cut(s)	574
StyD4I	CCNGG	6 cut(s)	274, 359, 704, 760, 855, 971
StyI	CCWWGG	2 cut(s)	774, 843
TaaI	ACNGT	4 cut(s)	355, 689, 901, 953
TaqI	TCGA	3 cut(s)	17, 911, 987
TasI	AATT	5 cut(s)	150, 162, 200, 584, 724
TauI	GCSGC	3 cut(s)	184, 468, 829
TfiI	GAWTC	1 cut(s)	908
Tru1I	TTAA	3 cut(s)	71, 870, 994
Tru9I	TTAA	3 cut(s)	71, 870, 994
TscAI	CASTG	5 cut(s)	360, 431, 692, 906, 964
TseFI	GTSAC	1 cut(s)	355
Tsp45I	GTSAC	1 cut(s)	355
TspDTI	ATGAA	1 cut(s)	851
TspGWI	ACGGA	1 cut(s)	474
TspRI	CASTG	5 cut(s)	360, 431, 692, 906, 964
VpaK11BI	GGWCC	3 cut(s)	259, 682, 764
XapI	RAATTY	3 cut(s)	162, 200, 584
XceI	RCATGY	5 cut(s)	32, 80, 178, 554, 968
XcmI	CCANNNNNNNNNTGG	1 cut(s)	311
XspI	CTAG	1 cut(s)	530
Zsp2I	ATGCAT	1 cut(s)	30

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.