FvH4_3g12120

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000491
Physical Location & Seq
Forward (+)
7184864 .. 7190922
6059 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g12120.t2

Sequence Viewer

Length: 2448 bp
ATGATTCGGGAATCATGTTGGACAGTGATAGATTTTGCATTGGTAGCATGCAAAATAGTCATATATTTAATTCGGAGAGCAGGGAAATTAGTTGCTCCCCTCATCTTAAAACCTGTAGAGAAGTGGGAACGGGCATTCGTAGTATCATGTATTGCAGTTTCCCTCGATCCTTTGTTTCTTTACATTCCGGTAGTCGATGAGACGAACAAGTGTCTTGCCATGGACAAGACGCTGCGAACTACAGTTCTCATTTTCCGATCACTCACGGATTTCCTTTTGCTTACTCATATCGTTGCTCAAATTCTTGATCGGGTGCACACCGACTACGAAATCCCTCCTCAGCTGGGCTCCAAACCCGGTTTCCACAAGGAGTTTGACTCCGATACCGAGGATCGCTCCGATTACTGGTTCTTTGTCAGCTCTCTCATAATCAAGTGTCTCGCTGTTCTTCCAATCCCACAGGTTGCGATACTGGTGGGCTTTTTCAGATTGGGAGGAACTGATTTCAGTATCACAAGAAAATTTCTGAACATGTCTCTTGTCCTCCAATATCTACCTAGGGTTAGTCAAATCTACCTATCCAGGAGTAAATTTAGCATGGGCGGAAAATGGGCTAGACCTGCATTCAATTTGTTTCTCTATATCCTTGCAAGTCATGTACTTGGTGCGTTATGGTATTTTTTTGCCATCGAACGAGAGACATCATGTTGGCACAGGGCTTGTAGAAAAACAGCAGGATGTATAGCTAGTGCTTACAAGTGCAGTGTGAGCGTCAGTGCCAGTACAAAAAATATAACATTGCTAAATCAACTATGCCCCATTACTTCACCCAATACTACAGAATTTGATTTTGGAATGTTCGTTAAGGTCCTCCAATCTGGTAACACAGGATCTGTAGATTTTCCAACAAAGATCTTCTACACTCTATGGTGGGGCTTGAAAAATCTAAGTAATTTTGGCACGAGTCTCGAAACAAGTAGCTACTTGTGGGAGAATTGTTTTGCAATTCTCATATGCGTTATTGGCTTGCTACTATTTCTATATCTCATTGGAAACATGCAGGTATTTATGCAGTCCGCAACTACAAATGCCTTGGAGTCTGAAAAATTGAGGCAGATAAAAATTGCAGAAAACAAAGGAGAAATTGTGAAGTGGATGAAAAGTAATAATCTCCCTGATGATACGAAGGAAACAATCAAGACAGCCATAAGAGAATACAAAGTTCTGGAAAAAAAGTTAATAATTGATGTGGATGTGAAATATTTTGTTAATGATACGCCATTCTCTATTCGTATCAAACAACCTATTTTGGAGCACCTTGGACTAGATTTGTTAAAAGGAGTACCTATATTTGCCAACACTCCAGATGACAAGTTGAGAAGTTTGTGCCACCTTATGATTCCAGTGACTTACAGCAGGAATGCTGCTTTTGTTAATGCCGACCAGCCACTTGATCAGATGATCTTCATTATACGAGGTGAAATTGAATTTGTAGGGACAACAGGTGATCCTTCATATCGCAGGCGTGAACACAAATACCTCGGAAAAGTTCTTGAAAGATGGATGGCGTTTAACTTCTTAAAGAGGTCTTATTGTGACCCTCTTCCTAATTTGGCCGGCGGATATATCAAAGCTTGTGATCTAGCAGTTGAAGCCCTTGCTCTCAAGGCCTGGGACATATTGCGTTTTGCCGAGCAGACTTTCGATGTCAAATCTCCACATTTGATAGCGCTTGCGTCTGCTCACAAGAGTACCGTTGATCATGAGACAAAAGAGGATCCGCTTATTCGTAGCGTGCTACCACTGCTATGGGAACCTCCAGCGGTTGGCTGGGTGAAACTCAACTGCAGTTATGTCTGGAATCCGGATTCTAAGTGGGCAGCTTTCGGTTGTGTATTGAGGAACGATAAAGGAGAACTGATTAATGTAGCAAGTGTTGGCAATAATAATCATAAGTATATTGACAAGTTTCATGCGGGAGCTTCCTGTATATGGTCCTTAATGAAGTTCTCTGTGCAACTTGTAAAAACTGGTCAGATCATTGTGGAGTCTGATGACAAGTTCCTCATCGATTTCCTGGAACAAGGGCCTATGCGCCCGGACGAAAGAGTGCGAGGCATCCTTCGTCCTGTGCTTCAAGATATTGCCAGTGAGGTTTCAAAGTTCAAGGTTTGTAAATTTGCGCACTGTAAGCGCCAAACTAATGCAGCAGCGGTTCGCGTAGCAGAACATGCTGTTCATGTTACTGAGCCTAAAACTTGGAAAAAGCCTGAAGAAATACCTGAACTGGTTGATGCCCTGAAGAAGGATAGTCCGCATCTATACAAGAGTTTACGAGAGCAAAAATTGGCTGATCAGAAGGTCAACATCGACAGTGGCTCGTCTACTTCGGCTGTTGGTGCTGCTCTTAGCTCTAAGGAAACAATTGTTGAAGTAAAAGAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

816

Amino Acids

92.25

Weight (kDa)

8.77

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 620 - 745 1e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2184
Acc36I ACCTGC 2 cut(s) 628, 1051
AccB7I CCANNNNNTGG 1 cut(s) 1826
AccI GTMKAC 1 cut(s) 2384
AccII CGCG 1 cut(s) 2220
AccIII TCCGGA 1 cut(s) 1864
AciI CCGC 8 cut(s) 603, 1077, 1620, 1781, 1823, 1976, 2213, 2315
AclWI GGATC 6 cut(s) 161, 399, 898, 1502, 1772, 1785
AcoI YGGCCR 1 cut(s) 1614
AcsI RAATTY 6 cut(s) 300, 521, 590, 842, 1487, 2177
AcuI CTGAAG 2 cut(s) 2292, 2321
AfaI GTAC 4 cut(s) 660, 784, 1344, 1753
AfeI AGCGCT 1 cut(s) 1731
AfiI CCNNNNNNNGG 5 cut(s) 344, 405, 1826, 1992, 2305
AflIII ACRYGT 1 cut(s) 531
AgsI TTSAA 9 cut(s) 628, 940, 1487, 1556, 1652, 2138, 2160, 2167, 2432
AjnI CCWGG 3 cut(s) 581, 1670, 2076
AjuI GAANNNNNNNTTGG 4 cut(s) 344, 376, 834, 866
AluBI AGCT 8 cut(s) 343, 420, 746, 981, 1634, 1883, 1982, 2412
AluI AGCT 8 cut(s) 343, 420, 746, 981, 1634, 1883, 1982, 2412
Alw21I GWGCWC 2 cut(s) 318, 1317
Alw26I GTCTC 6 cut(s) 194, 443, 540, 692, 971, 1760
Alw44I GTGCAC 1 cut(s) 314
AlwI GGATC 6 cut(s) 161, 399, 898, 1502, 1772, 1785
AlwNI CAGNNNCTG 1 cut(s) 893
Aor13HI TCCGGA 1 cut(s) 1864
Aor51HI AGCGCT 1 cut(s) 1731
AoxI GGCC 3 cut(s) 1614, 1668, 2087
ApaLI GTGCAC 1 cut(s) 314
ApeKI GCWGC 6 cut(s) 232, 1424, 1880, 2207, 2210, 2402
ApoI RAATTY 6 cut(s) 300, 521, 590, 842, 1487, 2177
AseI ATTAAT 1 cut(s) 1923
AspA2I CCTAGG 1 cut(s) 557
AspLEI GCGC 4 cut(s) 1732, 2097, 2185, 2196
AspS9I GGNCC 3 cut(s) 868, 1995, 2087
AsuC2I CCSGG 2 cut(s) 357, 2099
AsuHPI GGTGA 4 cut(s) 819, 1490, 1517, 1846
AvaII GGWCC 2 cut(s) 868, 1995
AvrII CCTAGG 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 318
BaeI ACNNNNGTAYC 2 cut(s) 1735, 1768
BamHI GGATCC 1 cut(s) 1777
BanII GRGCYC 1 cut(s) 350
BauI CACGAG 1 cut(s) 961
Bbv12I GWGCWC 2 cut(s) 318, 1317
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 6 cut(s) 219, 1411, 1892, 2219, 2222, 2389
BccI CCATC 3 cut(s) 695, 1554, 1558
BcgI CGANNNNNNTGC 2 cut(s) 949, 983
BciT130I CCWGG 3 cut(s) 583, 1672, 2078
BclI TGATCA 3 cut(s) 1453, 1759, 2353
BcnI CCSGG 2 cut(s) 357, 2099
BcoDI GTCTC 6 cut(s) 194, 443, 540, 692, 971, 1760
BfaI CTAG 5 cut(s) 558, 615, 747, 1325, 1643
BfmI CTRYAG 5 cut(s) 114, 240, 837, 894, 1846
BfoI RGCGCY 2 cut(s) 1733, 2197
BfuAI ACCTGC 2 cut(s) 628, 1051
BglII AGATCT 1 cut(s) 912
BisI GCNGC 6 cut(s) 233, 1425, 1881, 2208, 2211, 2403
BlnI CCTAGG 1 cut(s) 557
BlsI GCNGC 6 cut(s) 234, 1426, 1882, 2209, 2212, 2404
Bme1390I CCNGG 5 cut(s) 357, 583, 1672, 2078, 2099
Bme18I GGWCC 2 cut(s) 868, 1995
BmgT120I GGNCC 3 cut(s) 868, 1995, 2087
BmiI GGNNCC 3 cut(s) 349, 1779, 1815
BmrFI CCNGG 5 cut(s) 357, 583, 1672, 2078, 2099
BmsI GCATC 3 cut(s) 2127, 2284, 2326
BplI GAGNNNNNCTC 4 cut(s) 362, 394, 380, 412
BpmI CTGGAG 2 cut(s) 1347, 1803
Bpu10I CCTNAGC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 1649
BpuMI CCSGG 2 cut(s) 357, 2099
Bsa29I ATCGAT 1 cut(s) 2070
BsaJI CCNNGG 7 cut(s) 219, 387, 557, 1092, 1318, 1540, 1671
BsaWI WCCGGW 2 cut(s) 187, 1864
BsaXI ACNNNNNCTCC 2 cut(s) 1131, 1161
Bsc4I CCNNNNNNNGG 5 cut(s) 344, 405, 1826, 1992, 2305
Bse118I RCCGGY 1 cut(s) 1616
Bse1I ACTGG 7 cut(s) 410, 477, 780, 1403, 2035, 2148, 2292
Bse3DI GCAATG 1 cut(s) 797
BseAI TCCGGA 1 cut(s) 1864
BseBI CCWGG 3 cut(s) 583, 1672, 2078
BseCI ATCGAT 1 cut(s) 2070
BseDI CCNNGG 7 cut(s) 219, 387, 557, 1092, 1318, 1540, 1671
BseGI GGATG 5 cut(s) 743, 1161, 1258, 1569, 2118
BseLI CCNNNNNNNGG 5 cut(s) 344, 405, 1826, 1992, 2305
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 2 cut(s) 353, 2238
BseNI ACTGG 7 cut(s) 410, 477, 780, 1403, 2035, 2148, 2292
BseRI GAGGAG 1 cut(s) 327
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 6 cut(s) 219, 1411, 1892, 2219, 2222, 2389
BseYI CCCAGC 2 cut(s) 343, 1830
BsgI GTGCAG 1 cut(s) 781
Bsh1236I CGCG 1 cut(s) 2220
BshFI GGCC 3 cut(s) 1616, 1670, 2089
BshVI ATCGAT 1 cut(s) 2070
BsiHKAI GWGCWC 2 cut(s) 318, 1317
BsiSI CCGG 5 cut(s) 188, 357, 1617, 1865, 2099
BslFI GGGAC 2 cut(s) 1510, 1688
BslI CCNNNNNNNGG 5 cut(s) 344, 405, 1826, 1992, 2305
BsmAI GTCTC 6 cut(s) 194, 443, 540, 692, 971, 1760
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 2 cut(s) 1510, 1688
BsmI GAATGC 3 cut(s) 134, 623, 1426
BsnI GGCC 3 cut(s) 1616, 1670, 2089
Bsp1286I GDGCHC 3 cut(s) 318, 350, 1317
Bsp13I TCCGGA 1 cut(s) 1864
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 8 cut(s) 603, 1077, 1620, 1781, 1823, 1976, 2213, 2315
BspANI GGCC 3 cut(s) 1616, 1670, 2089
BspCNI CTCAG 2 cut(s) 352, 2239
BspDI ATCGAT 1 cut(s) 2070
BspEI TCCGGA 1 cut(s) 1864
BspFNI CGCG 1 cut(s) 2220
BspHI TCATGA 1 cut(s) 1762
BspLI GGNNCC 3 cut(s) 349, 1779, 1815
BspMAI CTGCAG 1 cut(s) 1850
BspMI ACCTGC 2 cut(s) 628, 1051
BspPI GGATC 6 cut(s) 161, 399, 898, 1502, 1772, 1785
BsrDI GCAATG 1 cut(s) 797
BsrFI RCCGGY 1 cut(s) 1616
BsrI ACTGG 7 cut(s) 410, 477, 780, 1403, 2035, 2148, 2292
BssAI RCCGGY 1 cut(s) 1616
BssECI CCNNGG 7 cut(s) 219, 387, 557, 1092, 1318, 1540, 1671
BssSI CACGAG 1 cut(s) 961
BssT1I CCWWGG 4 cut(s) 219, 557, 1092, 1318
Bst2BI CACGAG 1 cut(s) 961
Bst2UI CCWGG 3 cut(s) 583, 1672, 2078
Bst4CI ACNGT 5 cut(s) 25, 244, 1756, 2189, 2375
Bst6I CTCTTC 1 cut(s) 1608
BstAPI GCANNNNNTGC 1 cut(s) 2231
BstC8I GCNNGC 6 cut(s) 49, 1028, 1523, 1618, 1734, 1796
BstDEI CTNAG 6 cut(s) 339, 947, 1872, 2247, 2408, 2415
BstDSI CCRYGG 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 2303
BstF5I GGATG 5 cut(s) 743, 1161, 1258, 1569, 2118
BstFNI CGCG 1 cut(s) 2220
BstH2I RGCGCY 2 cut(s) 1733, 2197
BstHHI GCGC 4 cut(s) 1732, 2097, 2185, 2196
BstMAI GTCTC 6 cut(s) 194, 443, 540, 692, 971, 1760
BstNI CCWGG 3 cut(s) 583, 1672, 2078
BstNSI RCATGY 4 cut(s) 51, 535, 1060, 2234
BstSCI CCNGG 5 cut(s) 355, 581, 1670, 2076, 2097
BstSFI CTRYAG 5 cut(s) 114, 240, 837, 894, 1846
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 1 cut(s) 2220
BstV1I GCAGC 6 cut(s) 219, 1411, 1892, 2219, 2222, 2389
BstX2I RGATCY 3 cut(s) 890, 912, 1777
BstXI CCANNNNNNTGG 1 cut(s) 1809
BstYI RGATCY 3 cut(s) 890, 912, 1777
Bsu15I ATCGAT 1 cut(s) 2070
BsuRI GGCC 3 cut(s) 1616, 1670, 2089
BsuTUI ATCGAT 1 cut(s) 2070
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 5 cut(s) 743, 1161, 1258, 1569, 2118
BtsI GCAGTG 2 cut(s) 769, 1802
BtsIMutI CAGTG 8 cut(s) 30, 769, 781, 1410, 1802, 2155, 2185, 2380
BveI ACCTGC 2 cut(s) 628, 1051
Cac8I GCNNGC 6 cut(s) 49, 1028, 1523, 1618, 1734, 1796
CaiI CAGNNNCTG 1 cut(s) 893
CciI TCATGA 1 cut(s) 1762
CfoI GCGC 4 cut(s) 1732, 2097, 2185, 2196
Cfr10I RCCGGY 1 cut(s) 1616
Cfr13I GGNCC 3 cut(s) 868, 1995, 2087
ClaI ATCGAT 1 cut(s) 2070
CseI GACGC 3 cut(s) 238, 760, 1725
Csp6I GTAC 4 cut(s) 659, 783, 1343, 1752
CviQI GTAC 4 cut(s) 659, 783, 1343, 1752
DdeI CTNAG 6 cut(s) 339, 947, 1872, 2247, 2408, 2415
EaeI YGGCCR 1 cut(s) 1614
Eam1104I CTCTTC 1 cut(s) 1608
EarI CTCTTC 1 cut(s) 1608
EciI GGCGGA 2 cut(s) 618, 1635
Eco130I CCWWGG 4 cut(s) 219, 557, 1092, 1318
Eco147I AGGCCT 1 cut(s) 1670
Eco24I GRGCYC 1 cut(s) 350
Eco47I GGWCC 2 cut(s) 868, 1995
Eco47III AGCGCT 1 cut(s) 1731
Eco57I CTGAAG 2 cut(s) 2292, 2321
EcoNI CCTNNNNNAGG 1 cut(s) 2303
EcoO109I RGGNCCY 2 cut(s) 868, 2087
EcoRII CCWGG 3 cut(s) 581, 1670, 2076
EcoT14I CCWWGG 4 cut(s) 219, 557, 1092, 1318
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 4 cut(s) 219, 557, 1092, 1318
Esp3I CGTCTC 1 cut(s) 194
FaqI GGGAC 2 cut(s) 1510, 1688
FauI CCCGC 1 cut(s) 1969
FauNDI CATATG 1 cut(s) 1013
FbaI TGATCA 3 cut(s) 1453, 1759, 2353
FblI GTMKAC 1 cut(s) 2384
Fnu4HI GCNGC 6 cut(s) 233, 1425, 1881, 2208, 2211, 2403
FokI GGATG 5 cut(s) 750, 1168, 1265, 1576, 2105
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 6 cut(s) 233, 1425, 1881, 2208, 2211, 2403
FspBI CTAG 5 cut(s) 558, 615, 747, 1325, 1643
FspI TGCGCA 1 cut(s) 2184
GlaI GCGC 4 cut(s) 1731, 2096, 2184, 2195
GluI GCNGC 6 cut(s) 233, 1425, 1881, 2208, 2211, 2403
GsaI CCCAGC 2 cut(s) 347, 1834
GsuI CTGGAG 2 cut(s) 1347, 1803
HaeII RGCGCY 2 cut(s) 1733, 2197
HaeIII GGCC 3 cut(s) 1616, 1670, 2089
HapII CCGG 5 cut(s) 188, 357, 1617, 1865, 2099
HgaI GACGC 3 cut(s) 238, 760, 1725
HhaI GCGC 4 cut(s) 1732, 2097, 2185, 2196
Hin6I GCGC 4 cut(s) 1730, 2095, 2183, 2194
HinP1I GCGC 4 cut(s) 1730, 2095, 2183, 2194
HincII GTYRAC 1 cut(s) 2365
HindII GTYRAC 1 cut(s) 2365
HindIII AAGCTT 1 cut(s) 1632
HpaII CCGG 5 cut(s) 188, 357, 1617, 1865, 2099
HphI GGTGA 4 cut(s) 819, 1490, 1517, 1846
Hpy166II GTNNAC 5 cut(s) 316, 1529, 2333, 2365, 2385
Hpy8I GTNNAC 5 cut(s) 316, 1529, 2333, 2365, 2385
HpyAV CCTTC 5 cut(s) 1180, 1521, 2132, 2299, 2353
HpyCH4III ACNGT 5 cut(s) 25, 244, 1756, 2189, 2375
HpyF3I CTNAG 6 cut(s) 339, 947, 1872, 2247, 2408, 2415
HspAI GCGC 4 cut(s) 1730, 2095, 2183, 2194
Kpn2I TCCGGA 1 cut(s) 1864
KroI GCCGGC 1 cut(s) 1616
KroNI GCCGGC 1 cut(s) 1618
Ksp22I TGATCA 3 cut(s) 1453, 1759, 2353
LmnI GCTCC 5 cut(s) 100, 353, 401, 1312, 1979
Lsp1109I GCAGC 6 cut(s) 219, 1411, 1892, 2219, 2222, 2389
LweI GCATC 3 cut(s) 2127, 2284, 2326
MaeI CTAG 5 cut(s) 558, 615, 747, 1325, 1643
MaeIII GTNAC 4 cut(s) 881, 1405, 1595, 2242
MboII GAAGA 6 cut(s) 440, 907, 1456, 1595, 2285, 2314
MfeI CAATTG 1 cut(s) 2424
MflI RGATCY 3 cut(s) 890, 912, 1777
MhlI GDGCHC 3 cut(s) 318, 350, 1317
MlyI GAGTC 4 cut(s) 371, 973, 1106, 2057
MmeI TCCRAC 1 cut(s) 929
MroI TCCGGA 1 cut(s) 1864
MroNI GCCGGC 1 cut(s) 1616
MslI CAYNNNNRTG 1 cut(s) 1807
MspA1I CMGCKG 3 cut(s) 343, 1823, 2213
MspI CCGG 5 cut(s) 188, 357, 1617, 1865, 2099
MspR9I CCNGG 5 cut(s) 357, 583, 1672, 2078, 2099
MunI CAATTG 1 cut(s) 2424
Mva1269I GAATGC 3 cut(s) 134, 623, 1426
MvaI CCWGG 3 cut(s) 583, 1672, 2078
MvnI CGCG 1 cut(s) 2220
NaeI GCCGGC 1 cut(s) 1618
NciI CCSGG 2 cut(s) 357, 2099
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 1013
NgoMIV GCCGGC 1 cut(s) 1616
NlaIV GGNNCC 3 cut(s) 349, 1779, 1815
NmeAIII GCCGAG 1 cut(s) 1717
NmuCI GTSAC 2 cut(s) 1405, 1595
NsbI TGCGCA 1 cut(s) 2184
NspI RCATGY 4 cut(s) 51, 535, 1060, 2234
PaeI GCATGC 1 cut(s) 51
PagI TCATGA 1 cut(s) 1762
PceI AGGCCT 1 cut(s) 1670
PciI ACATGT 1 cut(s) 531
PctI GAATGC 3 cut(s) 134, 623, 1426
PdiI GCCGGC 1 cut(s) 1618
PfeI GAWTC 5 cut(s) 4, 11, 1399, 1861, 1868
PflMI CCANNNNNTGG 1 cut(s) 1826
PfoI TCCNGGA 2 cut(s) 581, 2076
PkrI GCNGC 6 cut(s) 234, 1426, 1882, 2209, 2212, 2404
PleI GAGTC 4 cut(s) 371, 972, 1105, 2056
PpsI GAGTC 4 cut(s) 371, 972, 1105, 2056
PpuMI RGGWCCY 1 cut(s) 868
PscI ACATGT 1 cut(s) 531
PshBI ATTAAT 1 cut(s) 1923
Psp5II RGGWCCY 1 cut(s) 868
Psp6I CCWGG 3 cut(s) 581, 1670, 2076
PspFI CCCAGC 2 cut(s) 343, 1830
PspGI CCWGG 3 cut(s) 581, 1670, 2076
PspN4I GGNNCC 3 cut(s) 349, 1779, 1815
PspPI GGNCC 3 cut(s) 868, 1995, 2087
PspPPI RGGWCCY 1 cut(s) 868
PstI CTGCAG 1 cut(s) 1850
PstNI CAGNNNCTG 1 cut(s) 893
PsuI RGATCY 3 cut(s) 890, 912, 1777
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 4 cut(s) 660, 784, 1344, 1753
RsaNI GTAC 4 cut(s) 659, 783, 1343, 1752
RseI CAYNNNNRTG 1 cut(s) 1807
SatI GCNGC 6 cut(s) 233, 1425, 1881, 2208, 2211, 2403
Sau96I GGNCC 3 cut(s) 868, 1995, 2087
SchI GAGTC 4 cut(s) 371, 973, 1106, 2057
ScrFI CCNGG 5 cut(s) 357, 583, 1672, 2078, 2099
SduI GDGCHC 3 cut(s) 318, 350, 1317
SfaNI GCATC 3 cut(s) 2127, 2284, 2326
SfcI CTRYAG 5 cut(s) 114, 240, 837, 894, 1846
SinI GGWCC 2 cut(s) 868, 1995
SmiMI CAYNNNNRTG 1 cut(s) 1807
SmlI CTYRAG 1 cut(s) 1664
SmoI CTYRAG 1 cut(s) 1664
SphI GCATGC 1 cut(s) 51
SseBI AGGCCT 1 cut(s) 1670
SsiI CCGC 8 cut(s) 603, 1077, 1620, 1781, 1823, 1976, 2213, 2315
SspI AATATT 1 cut(s) 1262
SspMI CTAG 5 cut(s) 558, 615, 747, 1325, 1643
StuI AGGCCT 1 cut(s) 1670
StyD4I CCNGG 5 cut(s) 355, 581, 1670, 2076, 2097
StyI CCWWGG 4 cut(s) 219, 557, 1092, 1318
TaaI ACNGT 5 cut(s) 25, 244, 1756, 2189, 2375
TaqI TCGA 7 cut(s) 165, 195, 690, 969, 1704, 2070, 2370
TatI WGTACW 2 cut(s) 658, 782
TfiI GAWTC 5 cut(s) 4, 11, 1399, 1861, 1868
TscAI CASTG 8 cut(s) 30, 769, 781, 1410, 1809, 2155, 2192, 2380
TseFI GTSAC 2 cut(s) 1405, 1595
TseI GCWGC 6 cut(s) 232, 1424, 1880, 2207, 2210, 2402
Tsp45I GTSAC 2 cut(s) 1405, 1595
TspDTI ATGAA 6 cut(s) 1172, 1456, 1503, 1961, 2018, 2228
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 8 cut(s) 30, 769, 781, 1410, 1809, 2155, 2192, 2380
Van91I CCANNNNNTGG 1 cut(s) 1826
VneI GTGCAC 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 868, 1995
VspI ATTAAT 1 cut(s) 1923
XagI CCTNNNNNAGG 1 cut(s) 2303
XapI RAATTY 6 cut(s) 300, 521, 590, 842, 1487, 2177
XceI RCATGY 4 cut(s) 51, 535, 1060, 2234
XcmI CCANNNNNNNNNTGG 1 cut(s) 1827
XmaJI CCTAGG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 2384
XspI CTAG 5 cut(s) 558, 615, 747, 1325, 1643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.