Rh5DG143500

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000491
Physical Location & Seq
Forward (+)
14695899 .. 14706840
10942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG143500.1

Sequence Viewer

Length: 1605 bp
ATGGATAACGGAGAGATCCCAGAAGATAACGTTGTAGTAAGTGTTCCATCGTCACCGGGCAGTGTGGTTGGCATTGAAAGGCAAGATCCAGTCCCTCGAACTCCAATTAGGAAAGCATGGGCAACAGTTTCAACTCTAGTTGTGGACATATGTTCAGAAGCTCCCAATCTAATTTCGAGTTGTGTCGAAATGATTATGACATTGGCATTGATCGGAATTATCGGAATTCTAGGTCCATTCGCGTTAGTTGCAAAAAAGTGGAAAAGGGTATTCATACTATCATGTGTTGCAGTGTCATTTGATTCTTTGTTCCTTTACATTCCAATCATCGATCAGGGAAACAAGTGCCTTGACACGGACAAGACGTTGAGGAATGTAGCGCTTGTCCTCAGATCACTTACAGATATCGCCTTCATATTCCATATTATAGCTCTGATCCGTGATATGATGGACGAGGGCCCCAAATGGGATGACCAAGCGTCAGCGTCACTTTTGCCAAGAAATCTAAATCTTGTGCGAGAGGCGATGGCTAAGAAGATGCCGTGGTTATCTATCCACGTCGTAGTCAACTTTCTAGCTATTCTTCCCATCCCACAGGTGGCAATAGTAGTTGGCTCTTTCAGATTGGGAGGCTCTAAACATTTTCTCAAACAAACAATTATAAACGTCTTTCTTGTCTTCCAATATTTCCTGAGGATTTTTCAAATGTTCTTATCCATTAAGAAGGTCGGTGACACAGTTGGAAAATTGGTCAAAGGTTCATTCTACTTTCTCATGTACATCATTGCTAGCTATACATATATACAATCAGAATCTGAAAAGTTTGTGAAGGTTAGAGAGTCACGACGAACAAGAATTGATACCAAGCACAGAGATATAGAAAAGTGGATGTCAAGTCATGGTATTCCTAATAATATGAGGTCTGCTGTCAGGAAAAATATTAAAGAAGATTATCTAGTAAAAAACATTGATGAAGATGTGAATGCTGGTTTCATGTACTTGTGTACTCGCGGATGTGATGTCAAATCAAATCCACTTTGTTTAGTTTGCGTACAAGCATTGAAGAAAATACCCATTCTTCGAAAATTGGATGAACAAAAAGACTTCGAAGTGTTTCTTAGTGTGATAGCTTTTGGCAGTACATATATTCCAATTTTCATCATAAAGAAAGACAGCTACATTACTCGAGCCGGACAAGAACTTCGTGGTATGTACTTGATTATGGATGGAGTAATTGAATGCAAGGATACTGAAACAATGAACACCCAAACTATGCGTCGCCATGATTGTTGTGGAGAAGAACTCCTGCGTTGGGCAATATTGAACTATAAGAAAGAGCGAGAGCATCGCGATCCTCTTCCTATTTCAACAGTAGATGTCAAATGTCAAACGAAAACAGAAGCTTTTCAGTTCACTGCTGATGAGTTGATCGAAATTCTCAAGAAGTTAAACAATTCAGAGCAGTTGGAGGCGTTGGCCCACGATAGCAGCACCGAACAACGGATCGAGATCACAAGGCCAGGCCAGATAGAGAAACACGGAACTCAGAAACAGAGTATTACTGAGAAACAGAGTTACAGAGTAGCAAATACCTTTCTCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

534

Amino Acids

60.77

Weight (kDa)

8.74

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 662
AccII CGCG 3 cut(s) 242, 1011, 1350
AciI CCGC 1 cut(s) 1011
AclI AACGTT 1 cut(s) 30
AclWI GGATC 5 cut(s) 10, 80, 430, 1346, 1511
AcsI RAATTY 2 cut(s) 225, 1434
AfaI GTAC 6 cut(s) 779, 998, 1006, 1053, 1141, 1214
AfeI AGCGCT 1 cut(s) 381
AfiI CCNNNNNNNGG 5 cut(s) 355, 466, 598, 1312, 1500
AgsI TTSAA 7 cut(s) 77, 132, 704, 1063, 1238, 1324, 1368
AhdI GACNNNNNGTC 1 cut(s) 478
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 1519
AluBI AGCT 7 cut(s) 161, 431, 578, 792, 1130, 1176, 1403
AluI AGCT 7 cut(s) 161, 431, 578, 792, 1130, 1176, 1403
AlwI GGATC 5 cut(s) 10, 80, 430, 1346, 1511
AlwNI CAGNNNCTG 1 cut(s) 815
Ama87I CYCGRG 1 cut(s) 1185
Aor51HI AGCGCT 1 cut(s) 381
AoxI GGCC 4 cut(s) 457, 1476, 1517, 1522
ApaI GGGCCC 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 1488
ApoI RAATTY 2 cut(s) 225, 1434
ArsI GACNNNNNNTTYG 4 cut(s) 1185, 1217, 1261, 1293
Asp700I GAANNNNTTC 2 cut(s) 1113, 1404
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 4 cut(s) 233, 457, 458, 1477
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 2 cut(s) 45, 743
AsuII TTCGAA 2 cut(s) 1081, 1107
AsuNHI GCTAGC 1 cut(s) 788
AvaI CYCGRG 1 cut(s) 1185
AvaII GGWCC 1 cut(s) 233
AxyI CCTNAGG 1 cut(s) 692
BaeGI GKGCMC 1 cut(s) 461
BanII GRGCYC 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 670
BbvI GCAGC 1 cut(s) 1500
BccI CCATC 5 cut(s) 55, 442, 520, 596, 1220
BceAI ACGGC 1 cut(s) 526
BciT130I CCWGG 1 cut(s) 1521
BciVI GTATCC 1 cut(s) 1240
BcnI CCSGG 1 cut(s) 57
BfaI CTAG 6 cut(s) 137, 230, 575, 789, 956, 1603
BfoI RGCGCY 1 cut(s) 383
BfuI GTATCC 1 cut(s) 1240
BisI GCNGC 1 cut(s) 1489
BlsI GCNGC 1 cut(s) 1490
Bme1390I CCNGG 2 cut(s) 57, 1521
Bme18I GGWCC 1 cut(s) 233
BmeRI GACNNNNNGTC 1 cut(s) 478
BmeT110I CYCGRG 1 cut(s) 1185
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 4 cut(s) 233, 457, 458, 1477
BmiI GGNNCC 2 cut(s) 459, 460
BmrFI CCNGG 2 cut(s) 57, 1521
BmsI GCATC 2 cut(s) 528, 1354
BmtI GCTAGC 1 cut(s) 792
BpiI GAAGAC 1 cut(s) 670
BplI GAGNNNNNCTC 2 cut(s) 1287, 1319
Bpu14I TTCGAA 2 cut(s) 1081, 1107
BpuEI CTTGAG 1 cut(s) 1424
BpuMI CCSGG 1 cut(s) 57
Bsa29I ATCGAT 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 1508
BsaJI CCNNGG 1 cut(s) 542
Bsc4I CCNNNNNNNGG 5 cut(s) 355, 466, 598, 1312, 1500
Bse1I ACTGG 1 cut(s) 89
Bse21I CCTNAGG 1 cut(s) 692
Bse3DI GCAATG 1 cut(s) 783
Bse8I GATNNNNATC 1 cut(s) 1508
BseBI CCWGG 1 cut(s) 1521
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 542
BseGI GGATG 6 cut(s) 475, 588, 894, 1019, 1096, 1231
BseJI GATNNNNATC 1 cut(s) 1508
BseLI CCNNNNNNNGG 5 cut(s) 355, 466, 598, 1312, 1500
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 4 cut(s) 403, 683, 1554, 1559
BseNI ACTGG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 1 cut(s) 1500
Bsh1236I CGCG 3 cut(s) 242, 1011, 1350
BshFI GGCC 4 cut(s) 459, 1478, 1519, 1524
BshVI ATCGAT 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 1185
BsiSI CCGG 2 cut(s) 56, 1191
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 5 cut(s) 355, 466, 598, 1312, 1500
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 2 cut(s) 988, 1244
BsnI GGCC 4 cut(s) 459, 1478, 1519, 1524
BsoBI CYCGRG 1 cut(s) 1185
Bsp119I TTCGAA 2 cut(s) 1081, 1107
Bsp120I GGGCCC 1 cut(s) 457
Bsp1286I GDGCHC 1 cut(s) 461
Bsp1407I TGTACA 1 cut(s) 777
Bsp68I TCGCGA 1 cut(s) 1350
BspACI CCGC 1 cut(s) 1011
BspANI GGCC 4 cut(s) 459, 1478, 1519, 1524
BspCNI CTCAG 4 cut(s) 402, 684, 1555, 1558
BspDI ATCGAT 1 cut(s) 330
BspFNI CGCG 3 cut(s) 242, 1011, 1350
BspLI GGNNCC 2 cut(s) 459, 460
BspOI GCTAGC 1 cut(s) 792
BspPI GGATC 5 cut(s) 10, 80, 430, 1346, 1511
BspT104I TTCGAA 2 cut(s) 1081, 1107
BsrDI GCAATG 1 cut(s) 783
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 1521
Bst4CI ACNGT 3 cut(s) 127, 739, 1372
Bst6I CTCTTC 1 cut(s) 1362
BstAUI TGTACA 1 cut(s) 777
BstBI TTCGAA 2 cut(s) 1081, 1107
BstC8I GCNNGC 1 cut(s) 790
BstDEI CTNAG 6 cut(s) 389, 531, 692, 1118, 1545, 1563
BstDSI CCRYGG 1 cut(s) 542
BstF5I GGATG 6 cut(s) 475, 588, 894, 1019, 1096, 1231
BstFNI CGCG 3 cut(s) 242, 1011, 1350
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 1 cut(s) 382
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNI CCWGG 1 cut(s) 1521
BstSCI CCNGG 2 cut(s) 55, 1519
BstSLI GKGCMC 1 cut(s) 461
BstUI CGCG 3 cut(s) 242, 1011, 1350
BstV1I GCAGC 1 cut(s) 1500
BstV2I GAAGAC 1 cut(s) 670
BstX2I RGATCY 2 cut(s) 15, 85
BstYI RGATCY 2 cut(s) 15, 85
Bsu15I ATCGAT 1 cut(s) 330
Bsu36I CCTNAGG 1 cut(s) 692
BsuI GTATCC 1 cut(s) 1240
BsuRI GGCC 4 cut(s) 459, 1478, 1519, 1524
BsuTUI ATCGAT 1 cut(s) 330
BtgI CCRYGG 1 cut(s) 542
BtgZI GCGATG 2 cut(s) 539, 1331
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 6 cut(s) 475, 588, 894, 1019, 1096, 1231
BtsI GCAGTG 3 cut(s) 67, 297, 1413
BtsIMutI CAGTG 3 cut(s) 67, 297, 1413
BtuMI TCGCGA 1 cut(s) 1350
Cac8I GCNNGC 1 cut(s) 790
CaiI CAGNNNCTG 1 cut(s) 815
CfoI GCGC 1 cut(s) 382
Cfr13I GGNCC 4 cut(s) 233, 457, 458, 1477
ClaI ATCGAT 1 cut(s) 330
CseI GACGC 3 cut(s) 468, 474, 1265
Csp6I GTAC 6 cut(s) 778, 997, 1005, 1052, 1140, 1213
CviAII CATG 6 cut(s) 117, 282, 775, 899, 994, 1283
CviQI GTAC 6 cut(s) 778, 997, 1005, 1052, 1140, 1213
DdeI CTNAG 6 cut(s) 389, 531, 692, 1118, 1545, 1563
DriI GACNNNNNGTC 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 1362
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 1362
Eco24I GRGCYC 1 cut(s) 461
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 233
Eco47III AGCGCT 1 cut(s) 381
Eco81I CCTNAGG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 1185
EcoO109I RGGNCCY 2 cut(s) 457, 458
EcoRI GAATTC 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 1519
EcoRV GATATC 1 cut(s) 406
EcoT38I GRGCYC 1 cut(s) 461
FaeI CATG 6 cut(s) 120, 285, 778, 902, 997, 1286
FalI AAGNNNNNCTT 2 cut(s) 1101, 1133
FaqI GGGAC 1 cut(s) 77
FatI CATG 6 cut(s) 116, 281, 774, 898, 993, 1282
FauNDI CATATG 1 cut(s) 149
Fnu4HI GCNGC 1 cut(s) 1489
FokI GGATG 6 cut(s) 482, 575, 901, 1026, 1103, 1238
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 1 cut(s) 1489
FspBI CTAG 6 cut(s) 137, 230, 575, 789, 956, 1603
GlaI GCGC 1 cut(s) 381
GluI GCNGC 1 cut(s) 1489
HaeII RGCGCY 1 cut(s) 383
HaeIII GGCC 4 cut(s) 459, 1478, 1519, 1524
HapII CCGG 2 cut(s) 56, 1191
HgaI GACGC 3 cut(s) 468, 474, 1265
HhaI GCGC 1 cut(s) 382
Hin1II CATG 6 cut(s) 120, 285, 778, 902, 997, 1286
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 1401
HinfI GANTC 3 cut(s) 302, 812, 839
HpaII CCGG 2 cut(s) 56, 1191
HphI GGTGA 2 cut(s) 45, 743
Hpy166II GTNNAC 4 cut(s) 145, 568, 1005, 1413
Hpy188III TCNNGA 6 cut(s) 691, 843, 931, 1349, 1441, 1507
Hpy8I GTNNAC 4 cut(s) 145, 568, 1005, 1413
Hpy99I CGWCG 3 cut(s) 563, 849, 1281
HpyAV CCTTC 3 cut(s) 421, 718, 823
HpyCH4III ACNGT 3 cut(s) 127, 739, 1372
HpyCH4IV ACGT 4 cut(s) 30, 365, 558, 666
HpyCH4V TGCA 3 cut(s) 251, 290, 1242
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 6 cut(s) 389, 531, 692, 1118, 1545, 1563
HpySE526I ACGT 4 cut(s) 30, 365, 558, 666
Hsp92II CATG 6 cut(s) 120, 285, 778, 902, 997, 1286
HspAI GCGC 1 cut(s) 380
LmnI GCTCC 1 cut(s) 166
Lsp1109I GCAGC 1 cut(s) 1500
LweI GCATC 2 cut(s) 528, 1354
MaeI CTAG 6 cut(s) 137, 230, 575, 789, 956, 1603
MaeII ACGT 4 cut(s) 30, 365, 558, 666
MaeIII GTNAC 5 cut(s) 51, 486, 731, 840, 1574
MflI RGATCY 2 cut(s) 15, 85
MhlI GDGCHC 1 cut(s) 461
MlyI GAGTC 1 cut(s) 848
MmeI TCCRAC 2 cut(s) 721, 1446
MroXI GAANNNNTTC 2 cut(s) 1113, 1404
MseI TTAA 3 cut(s) 720, 942, 1448
MspI CCGG 2 cut(s) 56, 1191
MspR9I CCNGG 2 cut(s) 57, 1521
Mva1269I GAATGC 2 cut(s) 988, 1244
MvaI CCWGG 1 cut(s) 1521
MvnI CGCG 3 cut(s) 242, 1011, 1350
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 149
NheI GCTAGC 1 cut(s) 788
NlaIII CATG 6 cut(s) 120, 285, 778, 902, 997, 1286
NlaIV GGNNCC 2 cut(s) 459, 460
NmuCI GTSAC 4 cut(s) 51, 486, 731, 840
NruI TCGCGA 1 cut(s) 1350
NspV TTCGAA 2 cut(s) 1081, 1107
PaeR7I CTCGAG 1 cut(s) 1185
PcsI WCGNNNNNNNCGW 1 cut(s) 362
PctI GAATGC 2 cut(s) 988, 1244
PdmI GAANNNNTTC 2 cut(s) 1113, 1404
PfeI GAWTC 2 cut(s) 302, 812
PkrI GCNGC 1 cut(s) 1490
PleI GAGTC 1 cut(s) 847
PpsI GAGTC 1 cut(s) 847
PsiI TTATAA 1 cut(s) 662
Psp1406I AACGTT 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 1519
PspGI CCWGG 1 cut(s) 1519
PspN4I GGNNCC 2 cut(s) 459, 460
PspOMI GGGCCC 1 cut(s) 457
PspPI GGNCC 4 cut(s) 233, 457, 458, 1477
PspXI VCTCGAGB 1 cut(s) 1185
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 2 cut(s) 15, 85
RruI TCGCGA 1 cut(s) 1350
RsaI GTAC 6 cut(s) 779, 998, 1006, 1053, 1141, 1214
RsaNI GTAC 6 cut(s) 778, 997, 1005, 1052, 1140, 1213
SaqAI TTAA 3 cut(s) 720, 942, 1448
SatI GCNGC 1 cut(s) 1489
Sau96I GGNCC 4 cut(s) 233, 457, 458, 1477
SchI GAGTC 1 cut(s) 848
ScrFI CCNGG 2 cut(s) 57, 1521
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 2 cut(s) 528, 1354
Sfr274I CTCGAG 1 cut(s) 1185
SfuI TTCGAA 2 cut(s) 1081, 1107
SinI GGWCC 1 cut(s) 233
SlaI CTCGAG 1 cut(s) 1185
SmlI CTYRAG 2 cut(s) 1185, 1439
SmoI CTYRAG 2 cut(s) 1185, 1439
SsiI CCGC 1 cut(s) 1011
SspI AATATT 3 cut(s) 686, 940, 1320
SspMI CTAG 6 cut(s) 137, 230, 575, 789, 956, 1603
StyD4I CCNGG 2 cut(s) 55, 1519
TaaI ACNGT 3 cut(s) 127, 739, 1372
TaiI ACGT 4 cut(s) 33, 368, 561, 669
TaqI TCGA 9 cut(s) 97, 176, 186, 330, 1081, 1107, 1186, 1431, 1506
TatI WGTACW 5 cut(s) 777, 996, 1004, 1139, 1212
TfiI GAWTC 2 cut(s) 302, 812
Tru1I TTAA 3 cut(s) 720, 942, 1448
Tru9I TTAA 3 cut(s) 720, 942, 1448
TscAI CASTG 3 cut(s) 67, 297, 1420
TseFI GTSAC 4 cut(s) 51, 486, 731, 840
TseI GCWGC 1 cut(s) 1488
Tsp45I GTSAC 4 cut(s) 51, 486, 731, 840
TspDTI ATGAA 8 cut(s) 262, 403, 750, 982, 987, 1107, 1147, 1274
TspGWI ACGGA 5 cut(s) 24, 371, 428, 1516, 1554
TspRI CASTG 3 cut(s) 67, 297, 1420
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 2 cut(s) 225, 1434
XcmI CCANNNNNNNNNTGG 2 cut(s) 595, 1289
XhoI CTCGAG 1 cut(s) 1185
XmnI GAANNNNTTC 2 cut(s) 1113, 1404
XspI CTAG 6 cut(s) 137, 230, 575, 789, 956, 1603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.