Rw1G014680

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000491
Physical Location & Seq
Forward (+)
32798640 .. 32823818
25179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G014680.1

Sequence Viewer

Length: 3552 bp
ATGCTTACATGGGAAGAAGCGCGAGCAATTTTATCTTTAATTTGGGAGACATTCATATGCTTCTGCGAGTGGGTTCCGCATGTTCTAATTTGGATGGTTGCGGCTCCAATATTTGTTCCACTGGTTGCAATTCAGTGGGCATGTAAACCAATTGCAACTTGGCGAGCATGTAAATCAATTGCGATTGCAATTACCGCAATTCGGTGGGCATGTAAATCAATTGCGGTTGCAATTTGGTTGGCATGGAAATCAATTGCGGTTGCAATTCGGTGGGCATGCAAGTCAATTGCGGTTGCAATTCGGTGGGCATGGAAGGCTTTTCTTGACATCCCTTCAGCAAAGTGGGAACATGTATTTGTAATATCATGTATTGCGGTCTCACTGGATCCTCTGTTCTTTTACATTCCAATCATCGACGAGACAAATAAATGTCTTTCAATGGACAAAAAGATGAGAACTACAGCTCTCGTCTTCCGATCGCTTACGGATGCCCATTTAGTACTCCATATTAAAGATCAAATTTGTGATCGGGTCAACTCTGACTACCCCATCTGGTCCGACTCTGACTCCGACTCCGACACCTACTTCGACTCCCATTCTGGGGCACCATCGTCTTCATTGAAGAAGAGGATGCAGTGGTTGTCCTTCTCTATTATAGTTAACTGCCTTGCCATTCTTCCCATACCACAGGTGGCTACATCAGTTGGCTTTTACCCAATAGGAGCAGGCTCATCGGCCTCTGAAGTTTCTATTAAAAGAGCAACGATCAACCTTTCTCTTGCCCTCCAGTATCTGCCAAGGGTTTGTCAAGTCTACCTATCTAGTAAGAAGTTTAGAACAAGCGGAAGATGGATTAAACCTTCGCTGAATTTGTTTCTTTACATCCTTAGTAGCCACATATTTGCTGCTTTTTGGTACTTTTTTGCTATTGGACGAGAGATCTCATGCTGGTATCAAACCTGCAAAGACACTCCAGGATGTGTTGCTGATTTTGATTGTAGTAGCCCTGCCAATACTTCAAGAAATATAGCAGTCTTAAACCAATTTTGCCCTATAAATTCACCGAATAGTACAGTATTCGATTTTGGAATCTATCTTCCAATCCTCCAGTCTGGTAACACGGGGAGTGTTAGTTTTACAACAAAGTTCTTTTACTCTTTATGGTGGGGCTTAAAAAATCTAAGCAATTTTGGCACAAACCTCGAAACCAGTACCTACAAGTGGGAGAACTGTTTTGCAATTGTCATTTGTGTTAGTGGATTGCTACTATTTCTCTATTTCATCGGAAATATGCAGATATATATGGAGTTGGACACCACAAAATCTTTGAAGACCGAGGAATCAAGACGCCAAAAGATTAAAGCAAAGAAAGGAGAAATAAAAACGTGGATGTCGAATATTGGTATCTCTACAAATATGGAAACCAGAATCAAGAAGTACATCAAACAATACAATGTCGTGGAAAAGAACATAAAGGCAGATGTGGATGTGACATATTTTCTCAACGGCAATCGTTTTGGCAACGTCAGAGAGCCTCTCTTGGAGCATCTCGTACAAAATGCGGTAAAAGAGGTACCGTTGGTTCAGAAGATGGATGCAAGTGGGGTAAAGACCTTGTGCGGATATATGACGCCAGTTGTCTATGACAAAGACCGCATGCTTGAAGAACAGGCCGGAAACAAGCTTCTTCAGATGATCTTCATCATACAAGGTGAAATTGACCAATGTTGTACCGAAACTCAGATGACCAACAGCCATTTTAAGCGTCTAAGTAAAGGTGATTGTTATGGAATACAAATTCAAAATTGGGCAGCAAGGAACTACATGAGGTCTTATTATGACCCTCTTCCAACTTCCACCGGATGGGTCAGATGCCAAACAAGAGTAGAAGCCTTTGAACTCCAGGCCACGGACTTGATGCGATCTATTACCCTGCAGAACTTGAGGCAGTTGAAGATTGATTCTGAGCAGTCGGAAAAGTTGGTAGGCATTGAAGACAACACTGATCAGATACCAATCGAGGATGATCTACAATCAATTCCCCAGTATGAACAGTTAGACCCTGACTGGGTGAAGCTCAGTTGTAGTGTTGTGTGGAACACCGAAACTAAGAAGGCAGGAACAGGTTACGTTTGCAGGGATGCGCGAGGAGCTTTCTTGGGTGCTGAAAGTAGAGGTGGTTTAAAATTCAGGGACAGATTTTTGGCTGAAGCTGACAACATAAACAAAGCTATTGCATTTTTTACTAAGAACATGAATAAATTTCAAGGCGAGGGGCGGATTGTGGTGGAGTCTGATAACAAAGATCTCATTGAGTGCCTCTGTCAGCCTTTGGACTCAAATGAAAGAATATCTGACGTCCTTCGACCCGTCCTGGCAGAAATTAGCAAGTCGGTTTCAAAATTTAAACCTAAACCTTTTAAACATTGTAAGCGCCAAAATAATGCAGCAGCTAATTGCATAGCAAGGCATGCCGTGGATTCTCCTGATCCTTTTTCTTGCATTGGCAAAGCACCTGATTGGCTACTTCCTATCCTCGAGAAAGATAATCTTCAGGAGAATCCATCGTCAGAGCAAAACCAGGAGCTGAGAGTAGACATTGAACTTCAGGAGAAACCATCGTCAGAGCAAAACCAGGAGGTGAGAGTAGACATTAAACCTTCGGAACCACCGTCAGAGCAAAGCCAGGACATTGAACATCCGGATTCCGGATTCGTGAATTGCCGAGACCAAAAAGGAAACATGGATGCTGACCTTAATGTGGATGTTCTATTCGCTAATCCTCCAAGTGATATCAAACAATCCAAAAAACGCTATCTCGGCTTGAATGCCCTAAAGAAAGTACCCATGCTTCAAAAGATGCACGAATACGTGTTGGAAATGATCTGCAACCATCTCGAGCCAAAGGTGTACGAGAGGAATAGCTATGTTGTTAGAGCGAGAGAACCACTTGGTTTGATGATCTTCATTCTCCGAGGCACAATTGGAATGACTGACACGACTAGCAGCAATGCCACAACAACAGGTTCCTCGGAGATCACCGAGTCACGTGATGACAAGATCCTTAGGGAGGGGGATTTTTATGGAGAACAACTTCTGAGCTGGGCATCTCCCAACAATATTTCTTCTTCTGATAATCCCGTCATCTCAGCTATAGATGTCAGATGTCAAACGAAAGTGGAAGCCTTGATTCTCAAGGCCGAGGACTTGAAAAGTGTGGCCTCCAAATGCGGATCGCAGTGGAATTTCGACAATTGTATGAATTCTCAGCAGTTGGTTGATGATGGCAGAGATCGTGTGGCTAATACACCACCAACGTCCACTATTGAGGAGACTATCCGGCTTCATCAGGGTCATGACATAATTCTAGAGCAACTAGTTCTAATTAGAGAGACCATATTTCGGAGATTAGATGACCAGGGGCGGAAATTAGATGATCAGGGCCGGAAATTATCTGATCAGGAACGGAAATTAGACAAGATGGCTGCATTTTTAGCCAGAGAGGGTTACACAGGGATTACAGGCGCATCTTCTACTCCTTCAGCGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1183

Amino Acids

133.95

Weight (kDa)

6.76

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 697 - 825 3.8e-10 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2361
Acc36I ACCTGC 1 cut(s) 968
Acc65I GGTACC 1 cut(s) 1573
AccB1I GGYRCC 2 cut(s) 604, 1573
AccB7I CCANNNNNTGG 1 cut(s) 1863
AccI GTMKAC 3 cut(s) 813, 2595, 2649
AccII CGCG 2 cut(s) 22, 2146
AccIII TCCGGA 2 cut(s) 2701, 2708
AclWI GGATC 5 cut(s) 380, 393, 2483, 3055, 3241
AcsI RAATTY 9 cut(s) 519, 868, 1057, 1797, 2186, 2261, 2402, 3244, 3262
AcuI CTGAAG 7 cut(s) 318, 762, 1673, 2229, 2537, 2591, 3525
AcvI CACGTG 1 cut(s) 3050
AcyI GRCGYC 3 cut(s) 1348, 1631, 2358
AflIII ACRYGT 2 cut(s) 349, 2871
AhlI ACTAGT 1 cut(s) 3376
AjnI CCWGG 7 cut(s) 973, 1902, 2373, 2580, 2634, 2685, 3417
AloI GAACNNNNNNTCC 2 cut(s) 377, 409
Alw26I GTCTC 6 cut(s) 41, 382, 413, 2721, 3326, 3386
AlwI GGATC 5 cut(s) 380, 393, 2483, 3055, 3241
AlwNI CAGNNNCTG 2 cut(s) 793, 2587
Ama87I CYCGRG 2 cut(s) 2537, 2897
Aor13HI TCCGGA 2 cut(s) 2701, 2708
AoxI GGCC 6 cut(s) 735, 1671, 1905, 3198, 3219, 3442
ApeKI GCWGC 6 cut(s) 905, 1811, 2447, 2450, 3006, 3485
ApoI RAATTY 9 cut(s) 519, 868, 1057, 1797, 2186, 2261, 2402, 3244, 3262
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
Asp700I GAANNNNTTC 1 cut(s) 3093
Asp718I GGTACC 1 cut(s) 1573
AspLEI GCGC 5 cut(s) 22, 2146, 2436, 3527, 3547
AspS9I GGNCC 2 cut(s) 555, 3442
AsuHPI GGTGA 6 cut(s) 1053, 1724, 1790, 2083, 2653, 3031
AvaI CYCGRG 2 cut(s) 2537, 2897
AvaII GGWCC 1 cut(s) 555
AxyI CCTNAGG 1 cut(s) 3065
BaeGI GKGCMC 1 cut(s) 607
BamHI GGATCC 1 cut(s) 385
BanI GGYRCC 2 cut(s) 604, 1573
BarI GAAGNNNNNNTAC 2 cut(s) 2835, 2867
BbrPI CACGTG 1 cut(s) 3050
BbsI GAAGAC 4 cut(s) 463, 606, 1337, 2001
BbvI GCAGC 6 cut(s) 892, 1823, 2459, 2462, 3018, 3472
BceAI ACGGC 2 cut(s) 1522, 2459
BcgI CGANNNNNNTGC 6 cut(s) 714, 748, 1183, 1217, 2878, 2912
BciT130I CCWGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
BclI TGATCA 3 cut(s) 2005, 3436, 3457
BcoDI GTCTC 6 cut(s) 41, 382, 413, 2721, 3326, 3386
BcuI ACTAGT 1 cut(s) 3376
BfaI CTAG 4 cut(s) 822, 3003, 3368, 3377
BfmI CTRYAG 3 cut(s) 459, 1934, 3153
BfoI RGCGCY 2 cut(s) 2437, 3548
BfuAI ACCTGC 1 cut(s) 968
BglII AGATCT 2 cut(s) 939, 2305
BisI GCNGC 7 cut(s) 102, 906, 1812, 2448, 2451, 3007, 3486
BlsI GCNGC 7 cut(s) 103, 907, 1813, 2449, 2452, 3008, 3487
BmcAI AGTACT 1 cut(s) 501
Bme1390I CCNGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
Bme18I GGWCC 1 cut(s) 555
BmeT110I CYCGRG 2 cut(s) 2537, 2897
BmgT120I GGNCC 2 cut(s) 555, 3442
BmiI GGNNCC 7 cut(s) 75, 105, 387, 606, 1575, 2667, 3028
BmrFI CCNGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
BmrI ACTGGG 2 cut(s) 2038, 2077
BmuI ACTGGG 2 cut(s) 2038, 2077
BpiI GAAGAC 4 cut(s) 463, 606, 1337, 2001
BplI GAGNNNNNCTC 2 cut(s) 1521, 1553
BpmI CTGGAG 4 cut(s) 770, 957, 1091, 1886
BpuEI CTTGAG 2 cut(s) 1963, 3179
BsaAI YACGTR 2 cut(s) 2872, 3050
BsaBI GATNNNNATC 2 cut(s) 1700, 2015
BsaHI GRCGYC 3 cut(s) 1348, 1631, 2358
BsaI GGTCTC 3 cut(s) 382, 2721, 3386
BsaJI CCNNGG 8 cut(s) 797, 1335, 1908, 2475, 2974, 3030, 3201, 3418
BsaWI WCCGGW 3 cut(s) 1859, 2701, 2708
BsaXI ACNNNNNCTCC 8 cut(s) 551, 557, 575, 581, 587, 605, 1117, 1147
Bse1I ACTGG 8 cut(s) 126, 387, 787, 1108, 1209, 1634, 2044, 2072
Bse21I CCTNAGG 1 cut(s) 3065
Bse3DI GCAATG 1 cut(s) 3016
Bse8I GATNNNNATC 2 cut(s) 1700, 2015
BseAI TCCGGA 2 cut(s) 2701, 2708
BseBI CCWGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
BseDI CCNNGG 8 cut(s) 797, 1335, 1908, 2475, 2974, 3030, 3201, 3418
BseJI GATNNNNATC 2 cut(s) 1700, 2015
BseMI GCAATG 1 cut(s) 3016
BseMII CTCAG 7 cut(s) 1754, 1956, 2092, 2579, 3089, 3162, 3281
BseNI ACTGG 8 cut(s) 126, 387, 787, 1108, 1209, 1634, 2044, 2072
BseRI GAGGAG 2 cut(s) 2163, 3344
BseSI GKGCMC 1 cut(s) 607
BseXI GCAGC 6 cut(s) 892, 1823, 2459, 2462, 3018, 3472
BseYI CCCAGC 1 cut(s) 3102
Bsh1236I CGCG 2 cut(s) 22, 2146
Bsh1285I CGRYCG 1 cut(s) 479
BshFI GGCC 6 cut(s) 737, 1673, 1907, 3200, 3221, 3444
BshNI GGYRCC 2 cut(s) 604, 1573
BsiEI CGRYCG 1 cut(s) 479
BsiHKCI CYCGRG 2 cut(s) 2537, 2897
BsiSI CCGG 6 cut(s) 1674, 1860, 2702, 2709, 3340, 3445
BslFI GGGAC 1 cut(s) 2207
BsmAI GTCTC 6 cut(s) 41, 382, 413, 2721, 3326, 3386
BsmFI GGGAC 1 cut(s) 2207
BsmI GAATGC 1 cut(s) 2833
BsnI GGCC 6 cut(s) 737, 1673, 1907, 3200, 3221, 3444
Bso31I GGTCTC 3 cut(s) 382, 2721, 3386
BsoBI CYCGRG 2 cut(s) 2537, 2897
Bsp1286I GDGCHC 1 cut(s) 607
Bsp13I TCCGGA 2 cut(s) 2701, 2708
BspANI GGCC 6 cut(s) 737, 1673, 1907, 3200, 3221, 3444
BspCNI CTCAG 7 cut(s) 1753, 1957, 2091, 2580, 3090, 3161, 3280
BspEI TCCGGA 2 cut(s) 2701, 2708
BspFNI CGCG 2 cut(s) 22, 2146
BspHI TCATGA 1 cut(s) 3355
BspLI GGNNCC 7 cut(s) 75, 105, 387, 606, 1575, 2667, 3028
BspMAI CTGCAG 1 cut(s) 1938
BspMI ACCTGC 1 cut(s) 968
BspPI GGATC 5 cut(s) 380, 393, 2483, 3055, 3241
BspT107I GGYRCC 2 cut(s) 604, 1573
BspTNI GGTCTC 3 cut(s) 382, 2721, 3386
BsrDI GCAATG 1 cut(s) 3016
BsrI ACTGG 8 cut(s) 126, 387, 787, 1108, 1209, 1634, 2044, 2072
BssECI CCNNGG 8 cut(s) 797, 1335, 1908, 2475, 2974, 3030, 3201, 3418
BssNI GRCGYC 3 cut(s) 1348, 1631, 2358
BssT1I CCWWGG 1 cut(s) 797
Bst2UI CCWGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
Bst4CI ACNGT 5 cut(s) 1075, 1232, 1578, 2055, 2673
Bst6I CTCTTC 2 cut(s) 620, 1851
BstACI GRCGYC 3 cut(s) 1348, 1631, 2358
BstAPI GCANNNNNTGC 1 cut(s) 2471
BstBAI YACGTR 2 cut(s) 2872, 3050
BstC8I GCNNGC 6 cut(s) 24, 165, 277, 727, 1658, 2472
BstDSI CCRYGG 2 cut(s) 1908, 2475
BstENI CCTNNNNNAGG 1 cut(s) 3068
BstFNI CGCG 2 cut(s) 22, 2146
BstH2I RGCGCY 2 cut(s) 2437, 3548
BstHHI GCGC 5 cut(s) 22, 2146, 2436, 3527, 3547
BstMAI GTCTC 6 cut(s) 41, 382, 413, 2721, 3326, 3386
BstMCI CGRYCG 1 cut(s) 479
BstMWI GCNNNNNNNGC 7 cut(s) 194, 314, 1191, 2150, 2471, 2820, 3494
BstNI CCWGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
BstNSI RCATGY 8 cut(s) 83, 144, 171, 213, 279, 353, 1660, 2474
BstSCI CCNGG 7 cut(s) 973, 1902, 2373, 2580, 2634, 2685, 3417
BstSFI CTRYAG 3 cut(s) 459, 1934, 3153
BstSLI GKGCMC 1 cut(s) 607
BstUI CGCG 2 cut(s) 22, 2146
BstV1I GCAGC 6 cut(s) 892, 1823, 2459, 2462, 3018, 3472
BstV2I GAAGAC 4 cut(s) 463, 606, 1337, 2001
BstX2I RGATCY 4 cut(s) 385, 939, 2305, 3060
BstYI RGATCY 4 cut(s) 385, 939, 2305, 3060
Bsu36I CCTNAGG 1 cut(s) 3065
BsuRI GGCC 6 cut(s) 737, 1673, 1907, 3200, 3221, 3444
BtgI CCRYGG 2 cut(s) 1908, 2475
BtsI GCAGTG 2 cut(s) 641, 3245
BtsIMutI CAGTG 6 cut(s) 119, 140, 380, 641, 2001, 3245
BveI ACCTGC 1 cut(s) 968
Cac8I GCNNGC 6 cut(s) 24, 165, 277, 727, 1658, 2472
CaiI CAGNNNCTG 2 cut(s) 793, 2587
CciI TCATGA 1 cut(s) 3355
CfoI GCGC 5 cut(s) 22, 2146, 2436, 3527, 3547
Cfr13I GGNCC 2 cut(s) 555, 3442
CseI GACGC 3 cut(s) 1356, 1639, 1754
CspCI CAANNNNNGTGG 2 cut(s) 2937, 2972
DraI TTTAAA 3 cut(s) 2184, 2407, 2422
Eam1104I CTCTTC 2 cut(s) 620, 1851
EarI CTCTTC 2 cut(s) 620, 1851
EciI GGCGGA 2 cut(s) 2293, 3439
Eco130I CCWWGG 1 cut(s) 797
Eco31I GGTCTC 3 cut(s) 382, 2721, 3386
Eco32I GATATC 1 cut(s) 2794
Eco47I GGWCC 1 cut(s) 555
Eco57I CTGAAG 7 cut(s) 318, 762, 1673, 2229, 2537, 2591, 3525
Eco72I CACGTG 1 cut(s) 3050
Eco81I CCTNAGG 1 cut(s) 3065
Eco88I CYCGRG 2 cut(s) 2537, 2897
EcoNI CCTNNNNNAGG 1 cut(s) 3068
EcoRI GAATTC 1 cut(s) 3262
EcoRII CCWGG 7 cut(s) 973, 1902, 2373, 2580, 2634, 2685, 3417
EcoRV GATATC 1 cut(s) 2794
EcoT14I CCWWGG 1 cut(s) 797
ErhI CCWWGG 1 cut(s) 797
FalI AAGNNNNNCTT 2 cut(s) 2535, 2567
FaqI GGGAC 1 cut(s) 2207
FauNDI CATATG 1 cut(s) 56
FbaI TGATCA 3 cut(s) 2005, 3436, 3457
FblI GTMKAC 3 cut(s) 813, 2595, 2649
Fnu4HI GCNGC 7 cut(s) 102, 906, 1812, 2448, 2451, 3007, 3486
Fsp4HI GCNGC 7 cut(s) 102, 906, 1812, 2448, 2451, 3007, 3486
FspBI CTAG 4 cut(s) 822, 3003, 3368, 3377
GlaI GCGC 5 cut(s) 21, 2145, 2435, 3526, 3546
GluI GCNGC 7 cut(s) 102, 906, 1812, 2448, 2451, 3007, 3486
GsaI CCCAGC 1 cut(s) 3106
GsuI CTGGAG 4 cut(s) 770, 957, 1091, 1886
HaeII RGCGCY 2 cut(s) 2437, 3548
HaeIII GGCC 6 cut(s) 737, 1673, 1907, 3200, 3221, 3444
HapII CCGG 6 cut(s) 1674, 1860, 2702, 2709, 3340, 3445
HgaI GACGC 3 cut(s) 1356, 1639, 1754
HhaI GCGC 5 cut(s) 22, 2146, 2436, 3527, 3547
Hin1I GRCGYC 3 cut(s) 1348, 1631, 2358
Hin6I GCGC 5 cut(s) 20, 2144, 2434, 3525, 3545
HinP1I GCGC 5 cut(s) 20, 2144, 2434, 3525, 3545
HincII GTYRAC 2 cut(s) 535, 661
HindII GTYRAC 2 cut(s) 535, 661
HindIII AAGCTT 1 cut(s) 1682
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 6 cut(s) 1674, 1860, 2702, 2709, 3340, 3445
HphI GGTGA 6 cut(s) 1053, 1724, 1790, 2083, 2653, 3031
Hpy166II GTNNAC 8 cut(s) 146, 535, 661, 814, 2596, 2650, 2911, 3321
Hpy8I GTNNAC 8 cut(s) 146, 535, 661, 814, 2596, 2650, 2911, 3321
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 8 cut(s) 307, 342, 655, 870, 2107, 2372, 2670, 3549
HpyCH4III ACNGT 5 cut(s) 1075, 1232, 1578, 2055, 2673
HpyCH4IV ACGT 7 cut(s) 1385, 1524, 2130, 2358, 2871, 3049, 3317
HpyF10VI GCNNNNNNNGC 7 cut(s) 194, 314, 1191, 2150, 2471, 2820, 3494
HpySE526I ACGT 7 cut(s) 1385, 1524, 2130, 2358, 2871, 3049, 3317
Hsp92I GRCGYC 3 cut(s) 1348, 1631, 2358
HspAI GCGC 5 cut(s) 20, 2144, 2434, 3525, 3545
Kpn2I TCCGGA 2 cut(s) 2701, 2708
KpnI GGTACC 1 cut(s) 1577
Ksp22I TGATCA 3 cut(s) 2005, 3436, 3457
KspAI GTTAAC 1 cut(s) 661
LmnI GCTCC 5 cut(s) 109, 722, 1543, 2150, 2584
Lsp1109I GCAGC 6 cut(s) 892, 1823, 2459, 2462, 3018, 3472
MaeI CTAG 4 cut(s) 822, 3003, 3368, 3377
MaeII ACGT 7 cut(s) 1385, 1524, 2130, 2358, 2871, 3049, 3317
MaeIII GTNAC 5 cut(s) 1115, 1489, 2126, 3045, 3506
MfeI CAATTG 8 cut(s) 150, 177, 219, 252, 285, 1239, 2982, 3253
MflI RGATCY 4 cut(s) 385, 939, 2305, 3060
MhlI GDGCHC 1 cut(s) 607
MlyI GAGTC 7 cut(s) 554, 560, 566, 584, 2300, 2330, 3053
MmeI TCCRAC 7 cut(s) 582, 594, 600, 1290, 1874, 1953, 2856
MroI TCCGGA 2 cut(s) 2701, 2708
MroXI GAANNNNTTC 1 cut(s) 3093
MslI CAYNNNNRTG 1 cut(s) 55
MspI CCGG 6 cut(s) 1674, 1860, 2702, 2709, 3340, 3445
MspR9I CCNGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
MunI CAATTG 8 cut(s) 150, 177, 219, 252, 285, 1239, 2982, 3253
Mva1269I GAATGC 1 cut(s) 2833
MvaI CCWGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
MvnI CGCG 2 cut(s) 22, 2146
MwoI GCNNNNNNNGC 7 cut(s) 194, 314, 1191, 2150, 2471, 2820, 3494
NdeI CATATG 1 cut(s) 56
NlaIV GGNNCC 7 cut(s) 75, 105, 387, 606, 1575, 2667, 3028
NmeAIII GCCGAG 3 cut(s) 2750, 2799, 3226
NmuCI GTSAC 2 cut(s) 1489, 3045
NspI RCATGY 8 cut(s) 83, 144, 171, 213, 279, 353, 1660, 2474
PaeI GCATGC 3 cut(s) 279, 1660, 2474
PaeR7I CTCGAG 2 cut(s) 2537, 2897
PagI TCATGA 1 cut(s) 3355
PciI ACATGT 1 cut(s) 349
PcsI WCGNNNNNNNCGW 2 cut(s) 1391, 2669
PctI GAATGC 1 cut(s) 2833
PdmI GAANNNNTTC 1 cut(s) 3093
PfeI GAWTC 9 cut(s) 1089, 1340, 1428, 1961, 2480, 2560, 2705, 2712, 3190
PflMI CCANNNNNTGG 1 cut(s) 1863
PfoI TCCNGGA 1 cut(s) 973
PkrI GCNGC 7 cut(s) 103, 907, 1813, 2449, 2452, 3008, 3487
Ple19I CGATCG 1 cut(s) 479
PleI GAGTC 7 cut(s) 554, 560, 566, 584, 2299, 2330, 3052
PmaCI CACGTG 1 cut(s) 3050
PmlI CACGTG 1 cut(s) 3050
PpsI GAGTC 7 cut(s) 554, 560, 566, 584, 2299, 2330, 3052
Ppu21I YACGTR 2 cut(s) 2872, 3050
PscI ACATGT 1 cut(s) 349
Psp6I CCWGG 7 cut(s) 973, 1902, 2373, 2580, 2634, 2685, 3417
PspCI CACGTG 1 cut(s) 3050
PspFI CCCAGC 1 cut(s) 3102
PspGI CCWGG 7 cut(s) 973, 1902, 2373, 2580, 2634, 2685, 3417
PspN4I GGNNCC 7 cut(s) 75, 105, 387, 606, 1575, 2667, 3028
PspPI GGNCC 2 cut(s) 555, 3442
PsrI GAACNNNNNNTAC 2 cut(s) 1566, 1598
PstI CTGCAG 1 cut(s) 1938
PstNI CAGNNNCTG 2 cut(s) 793, 2587
PsuI RGATCY 4 cut(s) 385, 939, 2305, 3060
PvuI CGATCG 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 55
SatI GCNGC 7 cut(s) 102, 906, 1812, 2448, 2451, 3007, 3486
Sau96I GGNCC 2 cut(s) 555, 3442
ScaI AGTACT 1 cut(s) 501
SchI GAGTC 7 cut(s) 554, 560, 566, 584, 2300, 2330, 3053
ScrFI CCNGG 7 cut(s) 975, 1904, 2375, 2582, 2636, 2687, 3419
SduI GDGCHC 1 cut(s) 607
SfcI CTRYAG 3 cut(s) 459, 1934, 3153
Sfr274I CTCGAG 2 cut(s) 2537, 2897
SinI GGWCC 1 cut(s) 555
SlaI CTCGAG 2 cut(s) 2537, 2897
SmiMI CAYNNNNRTG 1 cut(s) 55
SmlI CTYRAG 4 cut(s) 1942, 2537, 2897, 3194
SmoI CTYRAG 4 cut(s) 1942, 2537, 2897, 3194
SpeI ACTAGT 1 cut(s) 3376
SphI GCATGC 3 cut(s) 279, 1660, 2474
SspI AATATT 3 cut(s) 111, 1399, 3121
SspMI CTAG 4 cut(s) 822, 3003, 3368, 3377
StyD4I CCNGG 7 cut(s) 973, 1902, 2373, 2580, 2634, 2685, 3417
StyI CCWWGG 1 cut(s) 797
TaaI ACNGT 5 cut(s) 1075, 1232, 1578, 2055, 2673
TaiI ACGT 7 cut(s) 1388, 1527, 2133, 2361, 2874, 3052, 3320
TaqII GACCGA 1 cut(s) 1349
TatI WGTACW 3 cut(s) 499, 1070, 1437
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 9 cut(s) 1089, 1340, 1428, 1961, 2480, 2560, 2705, 2712, 3190
TscAI CASTG 6 cut(s) 126, 140, 387, 641, 2008, 3245
TseFI GTSAC 2 cut(s) 1489, 3045
TseI GCWGC 6 cut(s) 905, 1811, 2447, 2450, 3006, 3485
Tsp45I GTSAC 2 cut(s) 1489, 3045
TspGWI ACGGA 3 cut(s) 500, 1925, 3481
TspRI CASTG 6 cut(s) 126, 140, 387, 641, 2008, 3245
Van91I CCANNNNNTGG 1 cut(s) 1863
VpaK11BI GGWCC 1 cut(s) 555
XagI CCTNNNNNAGG 1 cut(s) 3068
XapI RAATTY 9 cut(s) 519, 868, 1057, 1797, 2186, 2261, 2402, 3244, 3262
XbaI TCTAGA 1 cut(s) 3367
XceI RCATGY 8 cut(s) 83, 144, 171, 213, 279, 353, 1660, 2474
XcmI CCANNNNNNNNNTGG 2 cut(s) 156, 688
XhoI CTCGAG 2 cut(s) 2537, 2897
XmiI GTMKAC 3 cut(s) 813, 2595, 2649
XmnI GAANNNNTTC 1 cut(s) 3093
XspI CTAG 4 cut(s) 822, 3003, 3368, 3377
ZraI GACGTC 1 cut(s) 2359
ZrmI AGTACT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.