Rh1AG175400

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000491
Physical Location & Seq
Reverse (-)
33555389 .. 33578565
23177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG175400.1

Sequence Viewer

Length: 2367 bp
ATGCAGATATATATGGAGTTGGACACCACAAAATCTTTGAAGACCCAGGAATCAAGACGCCAAAAGATTAAAGCAAAGAAAGCAGAAATAAAGAAGTGGATGTCAAATAATGGTATCTCTGGAAATATGGAAACCAGAATCAAGAAGTACATCAAACAATCCAATGTCTTGGAAAAGAACATAAAGGCAGATGTGGATGTGACATATTTTCTCGACGGCAATCGTTTTGGCAATTCTAGTATCAGAGAGCCTCTGTTAGAGCATCTCGTACAGAATGCGGTAAAACAGGTACCGTTGATTCAGAAGATGCAGGATGCAAGCGGGTTAAAGACCTTGTGCAGATATATGAAGCCAGTTGTCTATAACAAAGACCACATTCTTGAAGTACAGGAGCTTGTTCAGATGATCTTCATCATACAAGGTGAAATTGACCAATATTGTACCGAAACCAGCAGCAATTTTAAGCGTCTAAGTAAAGGTGATTGTTATGGATTACAAATTCAAGATTGGGCAGCAAGGAACTATATGAGGTCTTATTATGACCCTCTTCCAACTTTCACCGGATGGGTCAGATGCCAAACAAGAGTAGAAGCCTTTGAACTCAAGGCCATGGACTTGATGCAAATTATTACCCAGCAGAACTCGAGCCAGTCGAAGATTGATTCTGAGCAGTTGAAAAAGTTGGCAGGCATTGAAGACAACACTGATCAGATACCAAACGAGGATGATCTACAATCAATTCCCCAGTGTGAACAGTTAGACCCTGACTGGGTGAAGCTCAGTTGTAGTGTTGTGTGGGACCCGGAAACTAAGAAGGGGCGAACAGGTTACGTTTGCAGGGATGCGCGAGGAGCTTTCTTGGGTGCTGAAAGTAGAGGTGGTTTAGAATTCAGGGACAGATTTTTGGCTGAAGCTGACAACATAAACAAAGCCATTGCATTTTTTACTAGGAATAAATTTAAAGGCGGGGGGCGGATTGTGGTGGAGTCTGATAACAAAGATCTCATTGAGTGCCTCTGTATGCCTAATTTGGAATCAAATGAAAGACTAACTGACGTCCTTCGACCCGTCCTGGAAGAAATTATCAAGTCGGCTTCAAAATTTAAATCTAAACGTTTTAAACATTGTAAGCGCCAAAGCAATGCAGCAGCTAATTGCATAGCAAGGCATGCCGTGGATTCTCCTAATCCTTCTTCTTGGATTCGCAATGCACCTGATTGGCTGCTAAATATCCTCAACCAAGATAGCTTGCATCTTCAGGAGAAACCACCGTCAGAGCAAAACCAAGAGCTGAGAGTAGACATTGAACCGGAGAAACCACCGTCAAAGAAAAACCAAGAGCTGAGAGTAGACACTGAACCTCCGGAACCACCATCAAAGAAAAACCAGGAGATGAGAGTAGACATTGAACCTCCGGAACCACCGTCAAAGAAAAACCAGGAGCTGAGAGTCGACATTGAACCTCCGGAGCCACTGTCAGAACAAAACCAGGAGCTGACAGTAGACATTGAACATTCGGATTCCGGATTCATGAATTGCCTAGGCAAAAAAGGACACATGGATGCTGATGTTGATGTGGATGTTCTATTCGCTAATCCTCCAAGTGATATCAAACAATCCAAAAAACGCCATCTCGGCTTGAATGCCCTAAAGAAAGTACCCATACTTCAAAAGATGCACGAATACGTGTTGGAAATCATCTGCAACCATCTCGAGCCAAAGGTGTACGAGAGGAATAGCTATATTGTTAGAGCGAGAGAACCACTTGGTTTGATGGTCTTCATTCTCCGAGGCACAATTGGAATGACTGACACGACTAGCAGCAATGCCACAACAACAGGTTCCTCGGAGATCACAGAGTCACGTGATGACAAGATCCTTAGGGAGGGGGATTTTTATGGAGAACAGCTTCTGAGCTGGGCATCTCCCAACAATATTTCTTCTTCTGATAATCCCGTCATCTCAGCTATAGATGTCAGATGTCAAACAAAAGTGGAAGCCTTGATTCTCAAGGCCGAGGACTTGAAAAGTGTAGCCTCCAAATGCGGATCGCAGTGGAATTTCGACAATTGTATGAATTCTCAGCAGTTGGTTGATGATGGCAGAGATCGTGTGGCTAATACAGCACCAACGTCCACTATTGAGGAGACTATCCGGCTTCATCAGGGTCATGACATAATTCTAGAGCAACTAGTTCTAATTAGAGAGACCATATTCCGGAGATTAGATGACCAGGGACGGAAATTAGATGATCAGGGCCGGAAATTATCTGATCAGGAACGGAAATTAGACAAGATGGCTGCATTTTTAGCCAGAGAGGGTTACACAGGGATTACAGGCGCATCTTCTACTCCTTCAGCGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

788

Amino Acids

89.59

Weight (kDa)

6.0

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 265 - 391 8.2e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1059
Acc65I GGTACC 1 cut(s) 289
AccB1I GGYRCC 1 cut(s) 289
AccI GTMKAC 5 cut(s) 1299, 1350, 1401, 1452, 1503
AccII CGCG 1 cut(s) 847
AccIII TCCGGA 5 cut(s) 1363, 1414, 1465, 1523, 2217
AciI CCGC 5 cut(s) 278, 321, 966, 973, 2046
AclI AACGTT 1 cut(s) 1114
AclWI GGATC 2 cut(s) 1870, 2056
AcsI RAATTY 6 cut(s) 498, 887, 956, 1100, 2059, 2077
AcuI CTGAAG 3 cut(s) 930, 1241, 2340
AcvI CACGTG 1 cut(s) 1865
AcyI GRCGYC 2 cut(s) 58, 1056
AfaI GTAC 7 cut(s) 149, 270, 291, 387, 442, 1659, 1727
AfiI CCNNNNNNNGG 3 cut(s) 769, 1885, 2217
AflIII ACRYGT 1 cut(s) 1686
AhlI ACTAGT 1 cut(s) 2191
AjnI CCWGG 6 cut(s) 45, 1071, 1386, 1437, 1488, 2232
Alw26I GTCTC 2 cut(s) 2141, 2201
AlwI GGATC 2 cut(s) 1870, 2056
AlwNI CAGNNNCTG 3 cut(s) 1444, 1495, 1912
Ama87I CYCGRG 2 cut(s) 643, 1712
Aor13HI TCCGGA 5 cut(s) 1363, 1414, 1465, 1523, 2217
AoxI GGCC 3 cut(s) 606, 2013, 2257
ApeKI GCWGC 7 cut(s) 453, 512, 1145, 1148, 1222, 1821, 2300
ApoI RAATTY 6 cut(s) 498, 887, 956, 1100, 2059, 2077
Asp700I GAANNNNTTC 1 cut(s) 1908
Asp718I GGTACC 1 cut(s) 289
AspA2I CCTAGG 1 cut(s) 1540
AspLEI GCGC 4 cut(s) 847, 1134, 2342, 2362
AspS9I GGNCC 2 cut(s) 799, 2257
AsuC2I CCSGG 1 cut(s) 803
AsuHPI GGTGA 4 cut(s) 434, 491, 550, 784
AvaI CYCGRG 2 cut(s) 643, 1712
AvaII GGWCC 1 cut(s) 799
AvrII CCTAGG 1 cut(s) 1540
AxyI CCTNAGG 1 cut(s) 1880
BanI GGYRCC 1 cut(s) 289
BarI GAAGNNNNNNTAC 2 cut(s) 1650, 1682
BbrPI CACGTG 1 cut(s) 1865
BbsI GAAGAC 3 cut(s) 47, 702, 1771
BbvI GCAGC 7 cut(s) 465, 524, 1157, 1160, 1209, 1833, 2287
BccI CCATC 7 cut(s) 558, 1381, 1638, 1716, 1768, 2093, 2290
BceAI ACGGC 2 cut(s) 232, 1157
BcgI CGANNNNNNTGC 2 cut(s) 1693, 1727
BciT130I CCWGG 6 cut(s) 47, 1073, 1388, 1439, 1490, 2234
BclI TGATCA 3 cut(s) 706, 2251, 2272
BcnI CCSGG 1 cut(s) 803
BcoDI GTCTC 2 cut(s) 2141, 2201
BcuI ACTAGT 1 cut(s) 2191
BfaI CTAG 6 cut(s) 237, 948, 1541, 1818, 2183, 2192
BfmI CTRYAG 1 cut(s) 1968
BfoI RGCGCY 2 cut(s) 1135, 2363
BglI GCCNNNNNGGC 1 cut(s) 1635
BglII AGATCT 1 cut(s) 1000
BisI GCNGC 7 cut(s) 454, 513, 1146, 1149, 1223, 1822, 2301
BlnI CCTAGG 1 cut(s) 1540
BlsI GCNGC 7 cut(s) 455, 514, 1147, 1150, 1224, 1823, 2302
Bme1390I CCNGG 7 cut(s) 47, 803, 1073, 1388, 1439, 1490, 2234
Bme18I GGWCC 1 cut(s) 799
BmeT110I CYCGRG 2 cut(s) 643, 1712
BmgT120I GGNCC 2 cut(s) 799, 2257
BmiI GGNNCC 7 cut(s) 291, 800, 801, 1368, 1419, 1470, 1843
BmrFI CCNGG 7 cut(s) 47, 803, 1073, 1388, 1439, 1490, 2234
BmrI ACTGGG 2 cut(s) 739, 778
BmuI ACTGGG 2 cut(s) 739, 778
BpiI GAAGAC 3 cut(s) 47, 702, 1771
BpuEI CTTGAG 2 cut(s) 587, 1994
BpuMI CCSGG 1 cut(s) 803
BsaAI YACGTR 2 cut(s) 1687, 1865
BsaBI GATNNNNATC 1 cut(s) 410
BsaHI GRCGYC 2 cut(s) 58, 1056
BsaI GGTCTC 1 cut(s) 2201
BsaJI CCNNGG 8 cut(s) 45, 609, 1173, 1540, 1789, 1845, 2016, 2233
BsaWI WCCGGW 7 cut(s) 560, 1309, 1363, 1414, 1465, 1523, 2217
BsaXI ACNNNNNCTCC 4 cut(s) 1345, 1375, 1460, 1490
Bsc4I CCNNNNNNNGG 3 cut(s) 769, 1885, 2217
Bse1I ACTGG 4 cut(s) 353, 649, 745, 773
Bse21I CCTNAGG 1 cut(s) 1880
Bse3DI GCAATG 4 cut(s) 933, 1147, 1213, 1831
Bse8I GATNNNNATC 1 cut(s) 410
BseAI TCCGGA 5 cut(s) 1363, 1414, 1465, 1523, 2217
BseBI CCWGG 6 cut(s) 47, 1073, 1388, 1439, 1490, 2234
BseDI CCNNGG 8 cut(s) 45, 609, 1173, 1540, 1789, 1845, 2016, 2233
BseGI GGATG 8 cut(s) 105, 202, 319, 569, 730, 847, 1567, 1585
BseJI GATNNNNATC 1 cut(s) 410
BseLI CCNNNNNNNGG 3 cut(s) 769, 1885, 2217
BseMI GCAATG 4 cut(s) 933, 1147, 1213, 1831
BseMII CTCAG 8 cut(s) 657, 793, 1283, 1334, 1436, 1904, 1977, 2096
BseNI ACTGG 4 cut(s) 353, 649, 745, 773
BseRI GAGGAG 2 cut(s) 864, 2159
BseXI GCAGC 7 cut(s) 465, 524, 1157, 1160, 1209, 1833, 2287
BseYI CCCAGC 2 cut(s) 633, 1917
BsgI GTGCAG 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 847
BshFI GGCC 3 cut(s) 608, 2015, 2259
BshNI GGYRCC 1 cut(s) 289
BsiHKCI CYCGRG 2 cut(s) 643, 1712
BslFI GGGAC 3 cut(s) 812, 908, 2250
BslI CCNNNNNNNGG 3 cut(s) 769, 1885, 2217
BsmAI GTCTC 2 cut(s) 2141, 2201
BsmFI GGGAC 3 cut(s) 812, 908, 2250
BsmI GAATGC 2 cut(s) 280, 1648
BsnI GGCC 3 cut(s) 608, 2015, 2259
Bso31I GGTCTC 1 cut(s) 2201
BsoBI CYCGRG 2 cut(s) 643, 1712
Bsp13I TCCGGA 5 cut(s) 1363, 1414, 1465, 1523, 2217
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 5 cut(s) 278, 321, 966, 973, 2046
BspANI GGCC 3 cut(s) 608, 2015, 2259
BspCNI CTCAG 8 cut(s) 658, 792, 1284, 1335, 1437, 1905, 1976, 2095
BspEI TCCGGA 5 cut(s) 1363, 1414, 1465, 1523, 2217
BspFNI CGCG 1 cut(s) 847
BspHI TCATGA 2 cut(s) 1530, 2170
BspLI GGNNCC 7 cut(s) 291, 800, 801, 1368, 1419, 1470, 1843
BspPI GGATC 2 cut(s) 1870, 2056
BspT107I GGYRCC 1 cut(s) 289
BspTNI GGTCTC 1 cut(s) 2201
BsrDI GCAATG 4 cut(s) 933, 1147, 1213, 1831
BsrI ACTGG 4 cut(s) 353, 649, 745, 773
BssECI CCNNGG 8 cut(s) 45, 609, 1173, 1540, 1789, 1845, 2016, 2233
BssNI GRCGYC 2 cut(s) 58, 1056
BssT1I CCWWGG 2 cut(s) 609, 1540
Bst2UI CCWGG 6 cut(s) 47, 1073, 1388, 1439, 1490, 2234
Bst4CI ACNGT 7 cut(s) 294, 756, 1272, 1323, 1425, 1476, 1501
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 2 cut(s) 58, 1056
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstBAI YACGTR 2 cut(s) 1687, 1865
BstC8I GCNNGC 4 cut(s) 319, 688, 1170, 1250
BstDSI CCRYGG 2 cut(s) 609, 1173
BstENI CCTNNNNNAGG 1 cut(s) 1883
BstF5I GGATG 8 cut(s) 105, 202, 319, 569, 730, 847, 1567, 1585
BstFNI CGCG 1 cut(s) 847
BstH2I RGCGCY 2 cut(s) 1135, 2363
BstHHI GCGC 4 cut(s) 847, 1134, 2342, 2362
BstMAI GTCTC 2 cut(s) 2141, 2201
BstMWI GCNNNNNNNGC 6 cut(s) 80, 851, 1169, 1635, 2123, 2309
BstNI CCWGG 6 cut(s) 47, 1073, 1388, 1439, 1490, 2234
BstNSI RCATGY 1 cut(s) 1172
BstSCI CCNGG 7 cut(s) 45, 801, 1071, 1386, 1437, 1488, 2232
BstSFI CTRYAG 1 cut(s) 1968
BstUI CGCG 1 cut(s) 847
BstV1I GCAGC 7 cut(s) 465, 524, 1157, 1160, 1209, 1833, 2287
BstV2I GAAGAC 3 cut(s) 47, 702, 1771
BstX2I RGATCY 2 cut(s) 1000, 1875
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 2 cut(s) 1000, 1875
Bsu36I CCTNAGG 1 cut(s) 1880
BsuRI GGCC 3 cut(s) 608, 2015, 2259
BtgI CCRYGG 2 cut(s) 609, 1173
BtsCI GGATG 8 cut(s) 105, 202, 319, 569, 730, 847, 1567, 1585
BtsI GCAGTG 1 cut(s) 2060
BtsIMutI CAGTG 5 cut(s) 702, 752, 1353, 1472, 2060
Cac8I GCNNGC 4 cut(s) 319, 688, 1170, 1250
CaiI CAGNNNCTG 3 cut(s) 1444, 1495, 1912
CciI TCATGA 2 cut(s) 1530, 2170
CfoI GCGC 4 cut(s) 847, 1134, 2342, 2362
Cfr13I GGNCC 2 cut(s) 799, 2257
CseI GACGC 2 cut(s) 66, 455
Csp6I GTAC 7 cut(s) 148, 269, 290, 386, 441, 1658, 1726
CspCI CAANNNNNGTGG 2 cut(s) 1752, 1787
CviAII CATG 5 cut(s) 610, 1169, 1531, 1558, 2171
CviQI GTAC 7 cut(s) 148, 269, 290, 386, 441, 1658, 1726
DraI TTTAAA 3 cut(s) 961, 1105, 1120
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 988
Eco130I CCWWGG 2 cut(s) 609, 1540
Eco31I GGTCTC 1 cut(s) 2201
Eco32I GATATC 1 cut(s) 1609
Eco47I GGWCC 1 cut(s) 799
Eco57I CTGAAG 3 cut(s) 930, 1241, 2340
Eco72I CACGTG 1 cut(s) 1865
Eco81I CCTNAGG 1 cut(s) 1880
Eco88I CYCGRG 2 cut(s) 643, 1712
EcoNI CCTNNNNNAGG 1 cut(s) 1883
EcoO109I RGGNCCY 1 cut(s) 799
EcoRI GAATTC 2 cut(s) 887, 2077
EcoRII CCWGG 6 cut(s) 45, 1071, 1386, 1437, 1488, 2232
EcoRV GATATC 1 cut(s) 1609
EcoT14I CCWWGG 2 cut(s) 609, 1540
ErhI CCWWGG 2 cut(s) 609, 1540
FaeI CATG 5 cut(s) 613, 1172, 1534, 1561, 2174
FaqI GGGAC 3 cut(s) 812, 908, 2250
FatI CATG 5 cut(s) 609, 1168, 1530, 1557, 2170
FauI CCCGC 2 cut(s) 314, 959
FbaI TGATCA 3 cut(s) 706, 2251, 2272
FblI GTMKAC 5 cut(s) 1299, 1350, 1401, 1452, 1503
Fnu4HI GCNGC 7 cut(s) 454, 513, 1146, 1149, 1223, 1822, 2301
FokI GGATG 8 cut(s) 112, 209, 326, 576, 737, 854, 1574, 1592
Fsp4HI GCNGC 7 cut(s) 454, 513, 1146, 1149, 1223, 1822, 2301
FspBI CTAG 6 cut(s) 237, 948, 1541, 1818, 2183, 2192
GlaI GCGC 4 cut(s) 846, 1133, 2341, 2361
GluI GCNGC 7 cut(s) 454, 513, 1146, 1149, 1223, 1822, 2301
GsaI CCCAGC 2 cut(s) 637, 1921
HaeII RGCGCY 2 cut(s) 1135, 2363
HaeIII GGCC 3 cut(s) 608, 2015, 2259
HgaI GACGC 2 cut(s) 66, 455
HhaI GCGC 4 cut(s) 847, 1134, 2342, 2362
Hin1I GRCGYC 2 cut(s) 58, 1056
Hin1II CATG 5 cut(s) 613, 1172, 1534, 1561, 2174
Hin6I GCGC 4 cut(s) 845, 1132, 2340, 2360
HinP1I GCGC 4 cut(s) 845, 1132, 2340, 2360
HincII GTYRAC 1 cut(s) 1453
HindII GTYRAC 1 cut(s) 1453
HphI GGTGA 4 cut(s) 434, 491, 550, 784
Hpy166II GTNNAC 8 cut(s) 752, 1300, 1351, 1402, 1453, 1504, 1726, 2136
Hpy8I GTNNAC 8 cut(s) 752, 1300, 1351, 1402, 1453, 1504, 1726, 2136
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 4 cut(s) 808, 1070, 1200, 2364
HpyCH4III ACNGT 7 cut(s) 294, 756, 1272, 1323, 1425, 1476, 1501
HpyCH4IV ACGT 6 cut(s) 831, 1056, 1114, 1686, 1864, 2132
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 851, 1169, 1635, 2123, 2309
HpySE526I ACGT 6 cut(s) 831, 1056, 1114, 1686, 1864, 2132
Hsp92I GRCGYC 2 cut(s) 58, 1056
Hsp92II CATG 5 cut(s) 613, 1172, 1534, 1561, 2174
HspAI GCGC 4 cut(s) 845, 1132, 2340, 2360
KflI GGGWCCC 1 cut(s) 799
Kpn2I TCCGGA 5 cut(s) 1363, 1414, 1465, 1523, 2217
KpnI GGTACC 1 cut(s) 293
Ksp22I TGATCA 3 cut(s) 706, 2251, 2272
LmnI GCTCC 5 cut(s) 391, 851, 1441, 1468, 1492
Lsp1109I GCAGC 7 cut(s) 465, 524, 1157, 1160, 1209, 1833, 2287
MaeI CTAG 6 cut(s) 237, 948, 1541, 1818, 2183, 2192
MaeII ACGT 6 cut(s) 831, 1056, 1114, 1686, 1864, 2132
MaeIII GTNAC 4 cut(s) 199, 827, 1860, 2321
MfeI CAATTG 2 cut(s) 1797, 2068
MflI RGATCY 2 cut(s) 1000, 1875
MlyI GAGTC 3 cut(s) 995, 1458, 1868
MmeI TCCRAC 2 cut(s) 575, 1671
MroI TCCGGA 5 cut(s) 1363, 1414, 1465, 1523, 2217
MroXI GAANNNNTTC 1 cut(s) 1908
MseI TTAA 6 cut(s) 69, 326, 462, 960, 1104, 1119
MslI CAYNNNNRTG 1 cut(s) 1560
MspR9I CCNGG 7 cut(s) 47, 803, 1073, 1388, 1439, 1490, 2234
MunI CAATTG 2 cut(s) 1797, 2068
Mva1269I GAATGC 2 cut(s) 280, 1648
MvaI CCWGG 6 cut(s) 47, 1073, 1388, 1439, 1490, 2234
MvnI CGCG 1 cut(s) 847
MwoI GCNNNNNNNGC 6 cut(s) 80, 851, 1169, 1635, 2123, 2309
NciI CCSGG 1 cut(s) 803
NcoI CCATGG 1 cut(s) 609
NlaIII CATG 5 cut(s) 613, 1172, 1534, 1561, 2174
NlaIV GGNNCC 7 cut(s) 291, 800, 801, 1368, 1419, 1470, 1843
NmeAIII GCCGAG 2 cut(s) 1614, 2041
NmuCI GTSAC 2 cut(s) 199, 1860
NspI RCATGY 1 cut(s) 1172
PaeI GCATGC 1 cut(s) 1172
PaeR7I CTCGAG 2 cut(s) 643, 1712
PagI TCATGA 2 cut(s) 1530, 2170
PcsI WCGNNNNNNNCGW 1 cut(s) 650
PctI GAATGC 2 cut(s) 280, 1648
PdmI GAANNNNTTC 1 cut(s) 1908
PfoI TCCNGGA 1 cut(s) 1071
PkrI GCNGC 7 cut(s) 455, 514, 1147, 1150, 1224, 1823, 2302
PleI GAGTC 3 cut(s) 994, 1457, 1867
PmaCI CACGTG 1 cut(s) 1865
PmlI CACGTG 1 cut(s) 1865
PpsI GAGTC 3 cut(s) 994, 1457, 1867
Ppu21I YACGTR 2 cut(s) 1687, 1865
PpuMI RGGWCCY 1 cut(s) 799
Psp1406I AACGTT 1 cut(s) 1114
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 6 cut(s) 45, 1071, 1386, 1437, 1488, 2232
PspCI CACGTG 1 cut(s) 1865
PspFI CCCAGC 2 cut(s) 633, 1917
PspGI CCWGG 6 cut(s) 45, 1071, 1386, 1437, 1488, 2232
PspN4I GGNNCC 7 cut(s) 291, 800, 801, 1368, 1419, 1470, 1843
PspPI GGNCC 2 cut(s) 799, 2257
PspPPI RGGWCCY 1 cut(s) 799
PspXI VCTCGAGB 1 cut(s) 643
PstNI CAGNNNCTG 3 cut(s) 1444, 1495, 1912
PsuI RGATCY 2 cut(s) 1000, 1875
RsaI GTAC 7 cut(s) 149, 270, 291, 387, 442, 1659, 1727
RsaNI GTAC 7 cut(s) 148, 269, 290, 386, 441, 1658, 1726
RseI CAYNNNNRTG 1 cut(s) 1560
SalI GTCGAC 1 cut(s) 1451
SaqAI TTAA 6 cut(s) 69, 326, 462, 960, 1104, 1119
SatI GCNGC 7 cut(s) 454, 513, 1146, 1149, 1223, 1822, 2301
Sau96I GGNCC 2 cut(s) 799, 2257
SchI GAGTC 3 cut(s) 995, 1458, 1868
ScrFI CCNGG 7 cut(s) 47, 803, 1073, 1388, 1439, 1490, 2234
SfcI CTRYAG 1 cut(s) 1968
Sfr274I CTCGAG 2 cut(s) 643, 1712
SinI GGWCC 1 cut(s) 799
SlaI CTCGAG 2 cut(s) 643, 1712
SmiI ATTTAAAT 1 cut(s) 1105
SmiMI CAYNNNNRTG 1 cut(s) 1560
SmlI CTYRAG 4 cut(s) 602, 643, 1712, 2009
SmoI CTYRAG 4 cut(s) 602, 643, 1712, 2009
SpeI ACTAGT 1 cut(s) 2191
SphI GCATGC 1 cut(s) 1172
SsiI CCGC 5 cut(s) 278, 321, 966, 973, 2046
SspI AATATT 2 cut(s) 437, 1936
SspMI CTAG 6 cut(s) 237, 948, 1541, 1818, 2183, 2192
StyD4I CCNGG 7 cut(s) 45, 801, 1071, 1386, 1437, 1488, 2232
StyI CCWWGG 2 cut(s) 609, 1540
SwaI ATTTAAAT 1 cut(s) 1105
TaaI ACNGT 7 cut(s) 294, 756, 1272, 1323, 1425, 1476, 1501
TaiI ACGT 6 cut(s) 834, 1059, 1117, 1689, 1867, 2135
TaqI TCGA 7 cut(s) 213, 644, 653, 1063, 1452, 1713, 2064
TatI WGTACW 2 cut(s) 147, 385
Tru1I TTAA 6 cut(s) 69, 326, 462, 960, 1104, 1119
Tru9I TTAA 6 cut(s) 69, 326, 462, 960, 1104, 1119
TscAI CASTG 5 cut(s) 709, 752, 1360, 1479, 2060
TseFI GTSAC 2 cut(s) 199, 1860
TseI GCWGC 7 cut(s) 453, 512, 1145, 1148, 1222, 1821, 2300
Tsp45I GTSAC 2 cut(s) 199, 1860
TspDTI ATGAA 8 cut(s) 362, 400, 1056, 1519, 1547, 1771, 2090, 2150
TspGWI ACGGA 2 cut(s) 2254, 2296
TspRI CASTG 5 cut(s) 709, 752, 1360, 1479, 2060
VpaK11BI GGWCC 1 cut(s) 799
XagI CCTNNNNNAGG 1 cut(s) 1883
XapI RAATTY 6 cut(s) 498, 887, 956, 1100, 2059, 2077
XbaI TCTAGA 1 cut(s) 2182
XceI RCATGY 1 cut(s) 1172
XhoI CTCGAG 2 cut(s) 643, 1712
XmaJI CCTAGG 1 cut(s) 1540
XmiI GTMKAC 5 cut(s) 1299, 1350, 1401, 1452, 1503
XmnI GAANNNNTTC 1 cut(s) 1908
XspI CTAG 6 cut(s) 237, 948, 1541, 1818, 2183, 2192
ZraI GACGTC 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.