Rw2G042670

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000491
Physical Location & Seq
Forward (+)
69004878 .. 69013909
9032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G042670.1

Sequence Viewer

Length: 2382 bp
ATGGAAAACCAACAGGGCGAAAGCGTCGTCGTAAGCCTTCCATCGTCACTAGGTTGCTGTTCGGAGAGGTCAGCAGCTACAGCTTCAATTAGGAAATTGTGGTCAACAGTTACTTATCTAATTAGACAACTATGGTCAACCGTTATTTATCCAATTGGACAATGTTGCTGGGAACTGATATTTACCCCCAGTTGGGTTATATGTGAAGGTTTCTGCTTCGACGAAGTGTCATCTCCAACATGGTACATTGCATTTGTGATTACATTTGGGCTTTTATGGCTGGCACATATAATTCTTTCACCCATCAAATATACATGGAAACTGCTTAGAAGGAAGGCGATTCTGACATGTATTGCAGTGTCGTTGGATCCTTTCTTCTTTTATATTCCAATCATCAATCAGGAAAACAAGTGTCTTGGAGTCGACAAAAAACTGAGGAATACACTTCTTGTGTTGCGATCAGTGACGGATTTTGCTAGTGTATTGCATATTGTATCTCAAGTTCGGGATTGGACCATCGACCCAAACCCCGACTCCCACACCGACACCGAATCCTACTTTGACTCTGAACTTAGGCCGACAACATCCCTGTTGCAAAAACTGATGAAGTCCATAGTAGATGCAATAAATGGGACGATGCCTTGGTTATCTCTGTCTCTCGTGATTGATTTTCTTGCTCTTCTTCCAATTCCGCAGGTGGCAATAGTAGTTATCTTCTTCAAAATGGGGGGCTCTAAATTTTTCCTTCAAAGAAAGATTCTCAATGTCCTTCTAATATTTCAATACATCCCCAGGATATGTCAAATCTACCTATCAAGCAAGAAACTAGAAAGGGTTGGAATATGGGTTAAAGGTGCATTCAACTTTTTTCTTTACATCCTCGCTGGGCATGTACTTGCATCTTTTTGGTACTTTTTTTCCATTCAACGAGAGACATCATGTTGGCACCAAACTTGTAGAAATGTTATTGGATGCATATCTACTTATGAATGCGGTGGCAGTACTCCAAAGAATATATCACTTCTAAATGAATTGTGCCCTATAAGTTCACCGAATGATGCCGTATTTGATTTTGGAATATACCTTGATATCCTTCAATCTGGTAACACGGGATCAATAGATTTTTCGAGGAAATTCTTTTACTCTCTCTGGTGGGGCATCAGGAATTTAAGTAATTTTGGCACAAATCTCGAAACAAGCAGTTATGTCTGGGATAACTGTTTTGCAATTCTTATTGGTCTAATTGGCTTGCTTCTATTTTTGTATCTCATTGGAAATGTGCAGACATATATACAGTTGGCCACTACAAAGTCAGTGAAGATTGAAGAGAAAGCTGAGGAATTAAGACGATCGAGAATTAATAAGAAGTATCGAGAAATGAAAATGTGGATGTCAAGTAATGGTCTCCCCAAGGATTTAAAGACAACAATTAGAGAATATATTAAAAAATCTAACCTAGCGGAGAAATGCATAGATGCTCCAGTGAGTCTGAAAATTCTATACGATTCTACTAGAAAGGATGAAAGAATTGATATTGCACATTCTCTGGTCCACTATGTATTCGTGAAAGCGCTACAGAAAGGGGGTATGTTTGAGAGTTTCGATGACAGGGACTTGAAACGTGCCGTCGACGAGTTGTCGGGGTGGTATTTAATATACTCGGATTTTTCTCCATCTATCTACCCCGAGAATAGCTACATTGTTCGAGCGGGTGAACGGTGTAATCGGGTCATCTTAATCATAGAAGGCGTAATTGAATTGACCGACACAACGTCTAACGGTGCTGAAACAAGGGGTTCATCATCAGTGATTAAGACCGAGCGTCTTGAGAAAGGTGAGTACTATGGACAAGAATTGTTGAATTATTTGTCATCATTGATCGGTCAGCAGCTCCCGAATGGGGAAGGGCCTCCTATTTCGGCCCGAGATGTCAAATGTCGTACCGAAGTCGAAGCCCTCTGTATCAAGCCCGAATACTTTAATTGTTTACTACGACTTGTTAGCTGGGACTACATCTGGAGGCAGAACAAATGGGTGAAGCGGACCGATGACAACACTGATGAGACATTAAACAAGACAAACTTGCAGTCGGCAACTACAGAACTGGAGGAGATTGAGGAGTCAAAAAAGATGAAGATGAAGAACGTGGAACCAAGAGAAGACATTGCATCTACATCCACTACTGTTGAACAGAGACTTGAACAGATTATTCAGCTTCAGGGTGAGATGCTTCGCGAGCAGCTGCTTCTAGTCCGACAGATTCGTGATGATATGGCTACACAGGGACGAAGATTAGATGACATGGCTGCATTCTTAGCCACGTTGGGATATCCAGGCATATCTGCTCCTCCCGCACCTTCAACTCTTGAAGCGCGCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

793

Amino Acids

90.71

Weight (kDa)

7.01

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 685
Acc36I ACCTGC 1 cut(s) 685
AccB1I GGYRCC 1 cut(s) 945
AccBSI CCGCTC 1 cut(s) 1709
AccI GTMKAC 2 cut(s) 423, 1629
AccII CGCG 2 cut(s) 2235, 2374
AciI CCGC 6 cut(s) 692, 993, 1460, 1709, 2041, 2352
AclWI GGATC 3 cut(s) 362, 375, 1120
AcoI YGGCCR 1 cut(s) 1299
AcsI RAATTY 4 cut(s) 737, 1133, 1165, 1494
AcuI CTGAAG 1 cut(s) 2201
AfaI GTAC 6 cut(s) 245, 894, 911, 1003, 1841, 1942
AfeI AGCGCT 1 cut(s) 1572
AfiI CCNNNNNNNGG 3 cut(s) 192, 193, 1900
AflIII ACRYGT 1 cut(s) 347
AhdI GACNNNNNGTC 4 cut(s) 226, 1636, 1771, 1821
AjnI CCWGG 2 cut(s) 791, 2332
AluBI AGCT 8 cut(s) 77, 83, 1334, 1695, 1891, 2004, 2215, 2242
AluI AGCT 8 cut(s) 77, 83, 1334, 1695, 1891, 2004, 2215, 2242
Alw26I GTCTC 5 cut(s) 660, 926, 1409, 2057, 2188
AlwI GGATC 3 cut(s) 362, 375, 1120
Ama87I CYCGRG 2 cut(s) 1685, 1923
Aor51HI AGCGCT 1 cut(s) 1572
AoxI GGCC 4 cut(s) 575, 1299, 1907, 1920
ApeKI GCWGC 5 cut(s) 74, 1888, 2239, 2242, 2306
ApoI RAATTY 4 cut(s) 737, 1133, 1165, 1494
ArsI GACNNNNNNTTYG 4 cut(s) 12, 44, 2072, 2104
AseI ATTAAT 1 cut(s) 1359
AspLEI GCGC 3 cut(s) 1573, 2374, 2376
AspS9I GGNCC 5 cut(s) 513, 1549, 1907, 1921, 2043
AsuHPI GGTGA 6 cut(s) 291, 1041, 1724, 1847, 2047, 2234
AvaI CYCGRG 2 cut(s) 1685, 1923
AvaII GGWCC 3 cut(s) 513, 1549, 2043
BaeGI GKGCMC 1 cut(s) 1040
BalI TGGCCA 1 cut(s) 1301
BamHI GGATCC 1 cut(s) 367
BanI GGYRCC 1 cut(s) 945
BanII GRGCYC 1 cut(s) 734
BauI CACGAG 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 2166
BbvCI CCTCAGC 1 cut(s) 1335
BbvI GCAGC 5 cut(s) 86, 1900, 2229, 2251, 2293
BccI CCATC 4 cut(s) 49, 311, 524, 1681
BceAI ACGGC 2 cut(s) 1046, 1610
BcgI CGANNNNNNTGC 2 cut(s) 1171, 1205
BciT130I CCWGG 2 cut(s) 793, 2334
BcoDI GTCTC 5 cut(s) 660, 926, 1409, 2057, 2188
BfaI CTAG 6 cut(s) 50, 477, 827, 1457, 1512, 2249
BfmI CTRYAG 3 cut(s) 78, 1574, 2097
BfoI RGCGCY 1 cut(s) 1574
BfuAI ACCTGC 1 cut(s) 685
BisI GCNGC 5 cut(s) 75, 1889, 2240, 2243, 2307
BlsI GCNGC 5 cut(s) 76, 1890, 2241, 2244, 2308
BmcAI AGTACT 2 cut(s) 1003, 1841
Bme1390I CCNGG 2 cut(s) 793, 2334
Bme18I GGWCC 3 cut(s) 513, 1549, 2043
BmeRI GACNNNNNGTC 4 cut(s) 226, 1636, 1771, 1821
BmeT110I CYCGRG 2 cut(s) 1685, 1923
BmgT120I GGNCC 5 cut(s) 513, 1549, 1907, 1921, 2043
BmiI GGNNCC 3 cut(s) 369, 947, 2151
BmrFI CCNGG 2 cut(s) 793, 2334
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 9 cut(s) 610, 627, 908, 962, 1048, 1167, 1465, 2177, 2217
BmuI ACTGGG 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 2166
BpmI CTGGAG 3 cut(s) 1464, 2038, 2126
Bpu10I CCTNAGC 1 cut(s) 1335
BpuEI CTTGAG 2 cut(s) 483, 1847
BsaBI GATNNNNATC 1 cut(s) 976
BsaI GGTCTC 1 cut(s) 1409
BsaJI CCNNGG 3 cut(s) 641, 791, 1410
BsaXI ACNNNNNCTCC 2 cut(s) 518, 548
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 193, 1900
Bse1I ACTGG 3 cut(s) 189, 1481, 2109
Bse3DI GCAATG 2 cut(s) 246, 2163
Bse8I GATNNNNATC 1 cut(s) 976
BseBI CCWGG 2 cut(s) 793, 2334
BseDI CCNNGG 3 cut(s) 641, 791, 1410
BseGI GGATG 7 cut(s) 584, 786, 876, 977, 1395, 1525, 2174
BseJI GATNNNNATC 1 cut(s) 976
BseLI CCNNNNNNNGG 3 cut(s) 192, 193, 1900
BseMI GCAATG 2 cut(s) 246, 2163
BseMII CTCAG 2 cut(s) 425, 1326
BseNI ACTGG 3 cut(s) 189, 1481, 2109
BsePI GCGCGC 1 cut(s) 2372
BseRI GAGGAG 3 cut(s) 2123, 2132, 2337
BseSI GKGCMC 1 cut(s) 1040
BseXI GCAGC 5 cut(s) 86, 1900, 2229, 2251, 2293
BseYI CCCAGC 3 cut(s) 168, 884, 2004
BsgI GTGCAG 1 cut(s) 1301
Bsh1236I CGCG 2 cut(s) 2235, 2374
Bsh1285I CGRYCG 1 cut(s) 1352
BshFI GGCC 4 cut(s) 577, 1301, 1909, 1922
BshNI GGYRCC 1 cut(s) 945
BsiEI CGRYCG 1 cut(s) 1352
BsiHKCI CYCGRG 2 cut(s) 1685, 1923
BslFI GGGAC 4 cut(s) 646, 1625, 2021, 2298
BslI CCNNNNNNNGG 3 cut(s) 192, 193, 1900
BsmAI GTCTC 5 cut(s) 660, 926, 1409, 2057, 2188
BsmFI GGGAC 4 cut(s) 646, 1625, 2021, 2298
BsmI GAATGC 3 cut(s) 857, 995, 2309
BsnI GGCC 4 cut(s) 577, 1301, 1909, 1922
Bso31I GGTCTC 1 cut(s) 1409
BsoBI CYCGRG 2 cut(s) 1685, 1923
Bsp1286I GDGCHC 2 cut(s) 734, 1040
Bsp143I GATC 5 cut(s) 367, 458, 1112, 1349, 1878
Bsp68I TCGCGA 1 cut(s) 2235
BspACI CCGC 6 cut(s) 692, 993, 1460, 1709, 2041, 2352
BspANI GGCC 4 cut(s) 577, 1301, 1909, 1922
BspCNI CTCAG 2 cut(s) 426, 1327
BspFNI CGCG 2 cut(s) 2235, 2374
BspLI GGNNCC 3 cut(s) 369, 947, 2151
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 3 cut(s) 362, 375, 1120
BspQI GCTCTTC 1 cut(s) 684
BspT107I GGYRCC 1 cut(s) 945
BspTNI GGTCTC 1 cut(s) 1409
BsrBI CCGCTC 1 cut(s) 1709
BsrDI GCAATG 2 cut(s) 246, 2163
BsrI ACTGG 3 cut(s) 189, 1481, 2109
BssECI CCNNGG 3 cut(s) 641, 791, 1410
BssHII GCGCGC 1 cut(s) 2372
BssMI GATC 5 cut(s) 367, 458, 1112, 1349, 1878
BssSI CACGAG 1 cut(s) 659
BssT1I CCWWGG 2 cut(s) 641, 1410
Bst2BI CACGAG 1 cut(s) 659
Bst2UI CCWGG 2 cut(s) 793, 2334
Bst4CI ACNGT 7 cut(s) 109, 142, 1220, 1296, 1719, 1781, 2185
Bst6I CTCTTC 2 cut(s) 684, 1320
BstC8I GCNNGC 4 cut(s) 282, 1250, 2237, 2374
BstDEI CTNAG 5 cut(s) 326, 434, 572, 1335, 2314
BstF5I GGATG 7 cut(s) 584, 786, 876, 977, 1395, 1525, 2174
BstFNI CGCG 2 cut(s) 2235, 2374
BstH2I RGCGCY 1 cut(s) 1574
BstHHI GCGC 3 cut(s) 1573, 2374, 2376
BstKTI GATC 5 cut(s) 370, 461, 1115, 1352, 1881
BstMAI GTCTC 5 cut(s) 660, 926, 1409, 2057, 2188
BstMBI GATC 5 cut(s) 367, 458, 1112, 1349, 1878
BstMCI CGRYCG 1 cut(s) 1352
BstMWI GCNNNNNNNGC 5 cut(s) 80, 277, 2236, 2315, 2351
BstNI CCWGG 2 cut(s) 793, 2334
BstNSI RCATGY 2 cut(s) 351, 893
BstSCI CCNGG 2 cut(s) 791, 2332
BstSFI CTRYAG 3 cut(s) 78, 1574, 2097
BstSLI GKGCMC 1 cut(s) 1040
BstUI CGCG 2 cut(s) 2235, 2374
BstV1I GCAGC 5 cut(s) 86, 1900, 2229, 2251, 2293
BstV2I GAAGAC 1 cut(s) 2166
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuRI GGCC 4 cut(s) 577, 1301, 1909, 1922
BtsCI GGATG 7 cut(s) 584, 786, 876, 977, 1395, 1525, 2174
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 6 cut(s) 363, 468, 1320, 1488, 1812, 2055
BtuMI TCGCGA 1 cut(s) 2235
BveI ACCTGC 1 cut(s) 685
Cac8I GCNNGC 4 cut(s) 282, 1250, 2237, 2374
CfoI GCGC 3 cut(s) 1573, 2374, 2376
Cfr13I GGNCC 5 cut(s) 513, 1549, 1907, 1921, 2043
CpoI CGGWCCG 1 cut(s) 2043
CseI GACGC 2 cut(s) 13, 1811
Csp6I GTAC 6 cut(s) 244, 893, 910, 1002, 1840, 1941
CspCI CAANNNNNGTGG 2 cut(s) 2167, 2202
CspI CGGWCCG 1 cut(s) 2043
CviAII CATG 6 cut(s) 240, 315, 348, 890, 939, 2302
CviQI GTAC 6 cut(s) 244, 893, 910, 1002, 1840, 1941
DdeI CTNAG 5 cut(s) 326, 434, 572, 1335, 2314
DpnI GATC 5 cut(s) 369, 460, 1114, 1351, 1880
DpnII GATC 5 cut(s) 367, 458, 1112, 1349, 1878
DraI TTTAAA 1 cut(s) 1419
DriI GACNNNNNGTC 4 cut(s) 226, 1636, 1771, 1821
EaeI YGGCCR 1 cut(s) 1299
Eam1104I CTCTTC 2 cut(s) 684, 1320
Eam1105I GACNNNNNGTC 4 cut(s) 226, 1636, 1771, 1821
EarI CTCTTC 2 cut(s) 684, 1320
Eco130I CCWWGG 2 cut(s) 641, 1410
Eco24I GRGCYC 1 cut(s) 734
Eco31I GGTCTC 1 cut(s) 1409
Eco32I GATATC 2 cut(s) 1090, 2330
Eco47I GGWCC 3 cut(s) 513, 1549, 2043
Eco47III AGCGCT 1 cut(s) 1572
Eco57I CTGAAG 1 cut(s) 2201
Eco88I CYCGRG 2 cut(s) 1685, 1923
EcoO109I RGGNCCY 1 cut(s) 1907
EcoRII CCWGG 2 cut(s) 791, 2332
EcoRV GATATC 2 cut(s) 1090, 2330
EcoT14I CCWWGG 2 cut(s) 641, 1410
EcoT22I ATGCAT 2 cut(s) 977, 1472
EcoT38I GRGCYC 1 cut(s) 734
ErhI CCWWGG 2 cut(s) 641, 1410
FaeI CATG 6 cut(s) 243, 318, 351, 893, 942, 2305
FalI AAGNNNNNCTT 2 cut(s) 2066, 2098
FaqI GGGAC 4 cut(s) 646, 1625, 2021, 2298
FatI CATG 6 cut(s) 239, 314, 347, 889, 938, 2301
FauI CCCGC 2 cut(s) 1702, 2359
FblI GTMKAC 2 cut(s) 423, 1629
Fnu4HI GCNGC 5 cut(s) 75, 1889, 2240, 2243, 2307
FokI GGATG 7 cut(s) 571, 773, 863, 984, 1402, 1532, 2161
FriOI GRGCYC 1 cut(s) 734
Fsp4HI GCNGC 5 cut(s) 75, 1889, 2240, 2243, 2307
FspBI CTAG 6 cut(s) 50, 477, 827, 1457, 1512, 2249
GlaI GCGC 3 cut(s) 1572, 2373, 2375
GluI GCNGC 5 cut(s) 75, 1889, 2240, 2243, 2307
GsaI CCCAGC 3 cut(s) 172, 888, 2008
GsuI CTGGAG 3 cut(s) 1464, 2038, 2126
HaeII RGCGCY 1 cut(s) 1574
HaeIII GGCC 4 cut(s) 577, 1301, 1909, 1922
HgaI GACGC 2 cut(s) 13, 1811
HhaI GCGC 3 cut(s) 1573, 2374, 2376
Hin1II CATG 6 cut(s) 243, 318, 351, 893, 942, 2305
Hin6I GCGC 3 cut(s) 1571, 2372, 2374
HinP1I GCGC 3 cut(s) 1571, 2372, 2374
HincII GTYRAC 4 cut(s) 105, 138, 424, 1630
HindII GTYRAC 4 cut(s) 105, 138, 424, 1630
HphI GGTGA 6 cut(s) 291, 1041, 1724, 1847, 2047, 2234
Hpy166II GTNNAC 8 cut(s) 105, 138, 424, 1049, 1552, 1630, 1715, 1988
Hpy188I TCNGA 6 cut(s) 64, 345, 568, 1491, 1663, 2255
Hpy8I GTNNAC 8 cut(s) 105, 138, 424, 1049, 1552, 1630, 1715, 1988
Hpy99I CGWCG 5 cut(s) 29, 32, 224, 1631, 1634
HpyCH4III ACNGT 7 cut(s) 109, 142, 1220, 1296, 1719, 1781, 2185
HpyCH4IV ACGT 4 cut(s) 1621, 1772, 2145, 2321
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 277, 2236, 2315, 2351
HpyF3I CTNAG 5 cut(s) 326, 434, 572, 1335, 2314
HpySE526I ACGT 4 cut(s) 1621, 1772, 2145, 2321
Hsp92II CATG 6 cut(s) 243, 318, 351, 893, 942, 2305
HspAI GCGC 3 cut(s) 1571, 2372, 2374
Kzo9I GATC 5 cut(s) 367, 458, 1112, 1349, 1878
LguI GCTCTTC 1 cut(s) 684
LmnI GCTCC 3 cut(s) 1483, 1896, 2350
Lsp1109I GCAGC 5 cut(s) 86, 1900, 2229, 2251, 2293
LweI GCATC 9 cut(s) 610, 627, 908, 962, 1048, 1167, 1465, 2177, 2217
MaeI CTAG 6 cut(s) 50, 477, 827, 1457, 1512, 2249
MaeII ACGT 4 cut(s) 1621, 1772, 2145, 2321
MaeIII GTNAC 4 cut(s) 45, 109, 463, 1103
MalI GATC 5 cut(s) 369, 460, 1114, 1351, 1880
MbiI CCGCTC 1 cut(s) 1709
MboI GATC 5 cut(s) 367, 458, 1112, 1349, 1878
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 2 cut(s) 734, 1040
MlsI TGGCCA 1 cut(s) 1301
MluNI TGGCCA 1 cut(s) 1301
MlyI GAGTC 5 cut(s) 429, 527, 557, 1495, 2129
MmeI TCCRAC 4 cut(s) 260, 345, 817, 2278
Mox20I TGGCCA 1 cut(s) 1301
Mph1103I ATGCAT 2 cut(s) 977, 1472
MscI TGGCCA 1 cut(s) 1301
Msp20I TGGCCA 1 cut(s) 1301
MspA1I CMGCKG 1 cut(s) 2242
MspR9I CCNGG 2 cut(s) 793, 2334
MunI CAATTG 1 cut(s) 153
Mva1269I GAATGC 3 cut(s) 857, 995, 2309
MvaI CCWGG 2 cut(s) 793, 2334
MvnI CGCG 2 cut(s) 2235, 2374
MwoI GCNNNNNNNGC 5 cut(s) 80, 277, 2236, 2315, 2351
NdeII GATC 5 cut(s) 367, 458, 1112, 1349, 1878
NlaIII CATG 6 cut(s) 243, 318, 351, 893, 942, 2305
NlaIV GGNNCC 3 cut(s) 369, 947, 2151
NmuCI GTSAC 2 cut(s) 45, 463
NruI TCGCGA 1 cut(s) 2235
NsiI ATGCAT 2 cut(s) 977, 1472
NspI RCATGY 2 cut(s) 351, 893
PaqCI CACCTGC 1 cut(s) 685
PauI GCGCGC 1 cut(s) 2372
PciI ACATGT 1 cut(s) 347
PciSI GCTCTTC 1 cut(s) 684
PctI GAATGC 3 cut(s) 857, 995, 2309
PfeI GAWTC 5 cut(s) 340, 551, 757, 1505, 2260
PkrI GCNGC 5 cut(s) 76, 1890, 2241, 2244, 2308
Ple19I CGATCG 1 cut(s) 1352
PleI GAGTC 5 cut(s) 428, 527, 557, 1494, 2128
PpsI GAGTC 5 cut(s) 428, 527, 557, 1494, 2128
PscI ACATGT 1 cut(s) 347
PshBI ATTAAT 1 cut(s) 1359
Psp6I CCWGG 2 cut(s) 791, 2332
PspFI CCCAGC 3 cut(s) 168, 884, 2004
PspGI CCWGG 2 cut(s) 791, 2332
PspN4I GGNNCC 3 cut(s) 369, 947, 2151
PspPI GGNCC 5 cut(s) 513, 1549, 1907, 1921, 2043
PsrI GAACNNNNNNTAC 2 cut(s) 486, 518
PsuI RGATCY 1 cut(s) 367
PteI GCGCGC 1 cut(s) 2372
PvuI CGATCG 1 cut(s) 1352
PvuII CAGCTG 1 cut(s) 2242
RruI TCGCGA 1 cut(s) 2235
RsaI GTAC 6 cut(s) 245, 894, 911, 1003, 1841, 1942
RsaNI GTAC 6 cut(s) 244, 893, 910, 1002, 1840, 1941
Rsr2I CGGWCCG 1 cut(s) 2043
RsrII CGGWCCG 1 cut(s) 2043
SalI GTCGAC 2 cut(s) 422, 1628
SapI GCTCTTC 1 cut(s) 684
SatI GCNGC 5 cut(s) 75, 1889, 2240, 2243, 2307
Sau3AI GATC 5 cut(s) 367, 458, 1112, 1349, 1878
Sau96I GGNCC 5 cut(s) 513, 1549, 1907, 1921, 2043
ScaI AGTACT 2 cut(s) 1003, 1841
SchI GAGTC 5 cut(s) 429, 527, 557, 1495, 2129
ScrFI CCNGG 2 cut(s) 793, 2334
SduI GDGCHC 2 cut(s) 734, 1040
SfaNI GCATC 9 cut(s) 610, 627, 908, 962, 1048, 1167, 1465, 2177, 2217
SfcI CTRYAG 3 cut(s) 78, 1574, 2097
SgrDI CGTCGACG 1 cut(s) 1628
SinI GGWCC 3 cut(s) 513, 1549, 2043
SmlI CTYRAG 2 cut(s) 498, 1826
SmoI CTYRAG 2 cut(s) 498, 1826
SsiI CCGC 6 cut(s) 692, 993, 1460, 1709, 2041, 2352
SspI AATATT 1 cut(s) 777
SspMI CTAG 6 cut(s) 50, 477, 827, 1457, 1512, 2249
StyD4I CCNGG 2 cut(s) 791, 2332
StyI CCWWGG 2 cut(s) 641, 1410
TaaI ACNGT 7 cut(s) 109, 142, 1220, 1296, 1719, 1781, 2185
TaiI ACGT 4 cut(s) 1624, 1775, 2148, 2324
TaqII GACCGA 4 cut(s) 1778, 1832, 1871, 2060
TatI WGTACW 3 cut(s) 892, 1001, 1839
TfiI GAWTC 5 cut(s) 340, 551, 757, 1505, 2260
TscAI CASTG 6 cut(s) 363, 468, 1320, 1488, 1812, 2062
TseFI GTSAC 2 cut(s) 45, 463
TseI GCWGC 5 cut(s) 74, 1888, 2239, 2242, 2306
Tsp45I GTSAC 2 cut(s) 45, 463
TspDTI ATGAA 8 cut(s) 620, 1002, 1044, 1394, 1536, 1788, 2147, 2153
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 6 cut(s) 363, 468, 1320, 1488, 1812, 2062
VpaK11BI GGWCC 3 cut(s) 513, 1549, 2043
VspI ATTAAT 1 cut(s) 1359
XapI RAATTY 4 cut(s) 737, 1133, 1165, 1494
XceI RCATGY 2 cut(s) 351, 893
XmiI GTMKAC 2 cut(s) 423, 1629
XspI CTAG 6 cut(s) 50, 477, 827, 1457, 1512, 2249
ZrmI AGTACT 2 cut(s) 1003, 1841
Zsp2I ATGCAT 2 cut(s) 977, 1472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.