Rh5BG143700

voltage-gated potassium channel activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000491
Physical Location & Seq
Forward (+)
14925925 .. 14934971
9047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG143700.1

Sequence Viewer

Length: 1320 bp
ATGGAGTTGGCAACTACAAAATCATTGAAGTCTGAGCAATCAAGGCGCAAGAAGATTGCAGCAAAGAAAAAAGAAATAAAATTGTGGATGTCAACTAACATTACAGAAACATCGTCAAGTAATGGGGTCTCTCTGAATATGAGAAAAAGAATCAAGAAATACATCAAACAACACCACATTGTGGAGAAGGACATAAAGGCCGATGTAGATCTGAAATATTTTCTAGCCGATAATCGTTTTGCCACTGATCGTATCAGAGAGCCTCTGTGGGAGTATCTGGTCAAGAATGCAGCAGTAAAAAGTGTACCCATGCTTGAAAGGATGGATCAAAGCGGATGGAAAAGATTGTGCAGATATATGAAGCCGGTGATCTACAAAGAGAAAGAGGACATTGTAGAAAATGCAGGAGAAATGGTTGATCAGATGATCTTCATAATACAAGGTGAATTTGAGTGCACCAACACAATGCTCACCCACCCTAATCATCTAAAGAAAGGTGATTGTTATGGATCAAGACAAATTCAAAGCTGGGCAGCAAGGAACTACATGAGGTCTTATTATGACCCTCTTCCTACTTCCACCGGACGGGTCAGATGTCAAACAAAAGTTGAAGCCTTTGCACTCTGGGCCTTCGACTTCTTACGTTTTGTCCAGCAGAATTTGAGCCAAATTAAGTCTGTGCAGTTGAAAAAGTTGGCAGCGGCCGACGACATCAGTACTCCTCATCAGACAGACAACCAGGATGATCAACATTCTGTGGAGTCATGGGAATGTCCTAAGAGTGGCCTGGTGAAACTCAACTATAGTGTTGTCTGGGATTCTGTTTCTAAGAAGGCCAAAATGGGCTGCGTTTGCAGGGATAACCGAGGAGCGTTCATGCTTGCTGGAAGCAGCTGTACTTCACCATATAACGACGCCTTTCTTGCTGAAGCTTCTGGCATGGCCAAGGCTATCGAGTTCTGTCACCAGAATCGCCAGAATGTTATACTCAAAGCCGGCATTGGGATTGTTGTGGAGTCTGATAACCATGATCTCATTGATTGCCTCAATCGGCCTTTGGCTTCAAATGAAAGAATTAGTGAAACCCTTCGTTCCATCCTAGAAAGCATTCTAAATTTGGCTTCAAACTTTAAACCTAGATCTTTTATGCATTGCAACCGCCAAAGTAATACAGTTGCGAATCGCCTTGCAAACCATGCGGTGGATGCTGATGATCCTAATGTTTCAGCTTTCCGTGAAGCTCCTCCTGATTGGCTAATTCCTGCTCTGAAGGAGGATAGCCCCGAGTTTTACACAGAACACGACGAGGAAATGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
6PF2K Ank_2 Ank_4 Ank_5 F-box-like His_Phos_1 LRR_6 PAS_9 Pkinase Pkinase_Tyr RGS cNMP_binding

Protein Analysis

439

Amino Acids

50.18

Weight (kDa)

8.49

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 267 - 400 8.5e-15 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 181, 1201
AciI CCGC 4 cut(s) 333, 701, 1159, 1199
AclWI GGATC 3 cut(s) 333, 517, 1208
AcoI YGGCCR 2 cut(s) 702, 942
AcsI RAATTY 4 cut(s) 446, 519, 658, 1114
AcuI CTGAAG 2 cut(s) 948, 1289
AcyI GRCGYC 1 cut(s) 915
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 3 cut(s) 306, 718, 898
AfiI CCNNNNNNNGG 5 cut(s) 181, 585, 782, 1002, 1201
AgsI TTSAA 7 cut(s) 28, 317, 524, 611, 688, 1065, 1125
AjnI CCWGG 2 cut(s) 738, 786
AluBI AGCT 5 cut(s) 528, 894, 932, 1229, 1241
AluI AGCT 5 cut(s) 528, 894, 932, 1229, 1241
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 1 cut(s) 133
Alw44I GTGCAC 1 cut(s) 454
AlwI GGATC 3 cut(s) 333, 517, 1208
Ama87I CYCGRG 1 cut(s) 1283
AoxI GGCC 7 cut(s) 198, 627, 702, 784, 834, 942, 1052
ApaLI GTGCAC 1 cut(s) 454
ApeKI GCWGC 6 cut(s) 59, 290, 533, 698, 846, 891
ApoI RAATTY 4 cut(s) 446, 519, 658, 1114
Asp700I GAANNNNTTC 2 cut(s) 1086, 1107
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 627
AsuHPI GGTGA 7 cut(s) 379, 455, 463, 509, 802, 894, 956
AvaI CYCGRG 1 cut(s) 1283
BaeGI GKGCMC 1 cut(s) 458
BalI TGGCCA 1 cut(s) 944
BarI GAAGNNNNNNTAC 2 cut(s) 880, 912
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 6 cut(s) 71, 302, 545, 710, 833, 903
BccI CCATC 3 cut(s) 316, 330, 1103
BciT130I CCWGG 2 cut(s) 740, 788
BclI TGATCA 2 cut(s) 418, 745
BcoDI GTCTC 1 cut(s) 133
BfaI CTAG 3 cut(s) 224, 1100, 1137
BfmI CTRYAG 1 cut(s) 802
BglII AGATCT 2 cut(s) 208, 1139
BisI GCNGC 7 cut(s) 60, 291, 534, 699, 702, 847, 892
BlsI GCNGC 7 cut(s) 61, 292, 535, 700, 703, 848, 893
BmcAI AGTACT 1 cut(s) 718
Bme1390I CCNGG 2 cut(s) 740, 788
BmeT110I CYCGRG 1 cut(s) 1283
BmgT120I GGNCC 1 cut(s) 627
BmrFI CCNGG 2 cut(s) 740, 788
BmsI GCATC 1 cut(s) 1195
BsaBI GATNNNNATC 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 915
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 865, 945
BsaWI WCCGGW 1 cut(s) 581
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bsc4I CCNNNNNNNGG 5 cut(s) 181, 585, 782, 1002, 1201
Bse118I RCCGGY 2 cut(s) 364, 995
Bse3DI GCAATG 1 cut(s) 1150
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 2 cut(s) 740, 788
BseDI CCNNGG 2 cut(s) 865, 945
BseGI GGATG 6 cut(s) 93, 327, 341, 748, 1095, 1210
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 5 cut(s) 181, 585, 782, 1002, 1201
BseMI GCAATG 1 cut(s) 1150
BseMII CTCAG 1 cut(s) 24
BseRI GAGGAG 3 cut(s) 711, 882, 1233
BseSI GKGCMC 1 cut(s) 458
BseX3I CGGCCG 1 cut(s) 702
BseXI GCAGC 6 cut(s) 71, 302, 545, 710, 833, 903
BseYI CCCAGC 1 cut(s) 528
BsgI GTGCAG 2 cut(s) 370, 701
Bsh1285I CGRYCG 1 cut(s) 705
BshFI GGCC 7 cut(s) 200, 629, 704, 786, 836, 944, 1054
BsiEI CGRYCG 1 cut(s) 705
BsiHKAI GWGCWC 1 cut(s) 458
BsiHKCI CYCGRG 1 cut(s) 1283
BsiSI CCGG 3 cut(s) 365, 582, 996
BslI CCNNNNNNNGG 5 cut(s) 181, 585, 782, 1002, 1201
BsmAI GTCTC 1 cut(s) 133
BsmI GAATGC 2 cut(s) 292, 1107
BsnI GGCC 7 cut(s) 200, 629, 704, 786, 836, 944, 1054
Bso31I GGTCTC 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 1283
Bsp1286I GDGCHC 1 cut(s) 458
BspACI CCGC 4 cut(s) 333, 701, 1159, 1199
BspANI GGCC 7 cut(s) 200, 629, 704, 786, 836, 944, 1054
BspCNI CTCAG 1 cut(s) 25
BspPI GGATC 3 cut(s) 333, 517, 1208
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 1150
BsrFI RCCGGY 2 cut(s) 364, 995
BssAI RCCGGY 2 cut(s) 364, 995
BssECI CCNNGG 2 cut(s) 865, 945
BssNI GRCGYC 1 cut(s) 915
BssT1I CCWWGG 1 cut(s) 945
Bst2UI CCWGG 2 cut(s) 740, 788
Bst4CI ACNGT 1 cut(s) 1174
Bst6I CTCTTC 1 cut(s) 573
BstACI GRCGYC 1 cut(s) 915
BstAPI GCANNNNNTGC 1 cut(s) 1196
BstC8I GCNNGC 2 cut(s) 882, 997
BstDEI CTNAG 3 cut(s) 33, 777, 828
BstF5I GGATG 6 cut(s) 93, 327, 341, 748, 1095, 1210
BstHHI GCGC 1 cut(s) 48
BstMAI GTCTC 1 cut(s) 133
BstMCI CGRYCG 1 cut(s) 705
BstMWI GCNNNNNNNGC 6 cut(s) 43, 626, 852, 923, 1196, 1205
BstNI CCWGG 2 cut(s) 740, 788
BstSCI CCNGG 2 cut(s) 738, 786
BstSFI CTRYAG 1 cut(s) 802
BstSLI GKGCMC 1 cut(s) 458
BstV1I GCAGC 6 cut(s) 71, 302, 545, 710, 833, 903
BstX2I RGATCY 2 cut(s) 208, 1139
BstYI RGATCY 2 cut(s) 208, 1139
BstZI CGGCCG 1 cut(s) 702
BsuRI GGCC 7 cut(s) 200, 629, 704, 786, 836, 944, 1054
BtsCI GGATG 6 cut(s) 93, 327, 341, 748, 1095, 1210
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 882, 997
CfoI GCGC 1 cut(s) 48
Cfr10I RCCGGY 2 cut(s) 364, 995
Cfr13I GGNCC 1 cut(s) 627
CseI GACGC 1 cut(s) 923
Csp6I GTAC 3 cut(s) 305, 717, 897
CviAII CATG 7 cut(s) 310, 547, 765, 877, 940, 1028, 1196
CviQI GTAC 3 cut(s) 305, 717, 897
DdeI CTNAG 3 cut(s) 33, 777, 828
DraI TTTAAA 1 cut(s) 1132
DraIII CACNNNGTG 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 702, 942
EagI CGGCCG 1 cut(s) 702
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
EclXI CGGCCG 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 945
Eco31I GGTCTC 1 cut(s) 133
Eco52I CGGCCG 1 cut(s) 702
Eco57I CTGAAG 2 cut(s) 948, 1289
Eco88I CYCGRG 1 cut(s) 1283
EcoRII CCWGG 2 cut(s) 738, 786
EcoT14I CCWWGG 1 cut(s) 945
EcoT22I ATGCAT 1 cut(s) 1152
ErhI CCWWGG 1 cut(s) 945
FaeI CATG 7 cut(s) 313, 550, 768, 880, 943, 1031, 1199
FatI CATG 7 cut(s) 309, 546, 764, 876, 939, 1027, 1195
FbaI TGATCA 2 cut(s) 418, 745
Fnu4HI GCNGC 7 cut(s) 60, 291, 534, 699, 702, 847, 892
FokI GGATG 6 cut(s) 100, 334, 348, 755, 1082, 1217
Fsp4HI GCNGC 7 cut(s) 60, 291, 534, 699, 702, 847, 892
FspBI CTAG 3 cut(s) 224, 1100, 1137
GlaI GCGC 1 cut(s) 47
GluI GCNGC 7 cut(s) 60, 291, 534, 699, 702, 847, 892
GsaI CCCAGC 1 cut(s) 532
HaeIII GGCC 7 cut(s) 200, 629, 704, 786, 836, 944, 1054
HapII CCGG 3 cut(s) 365, 582, 996
HgaI GACGC 1 cut(s) 923
HhaI GCGC 1 cut(s) 48
Hin1I GRCGYC 1 cut(s) 915
Hin1II CATG 7 cut(s) 313, 550, 768, 880, 943, 1031, 1199
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 930
HinfI GANTC 6 cut(s) 150, 761, 818, 970, 1016, 1180
HpaII CCGG 3 cut(s) 365, 582, 996
HphI GGTGA 7 cut(s) 379, 455, 463, 509, 802, 894, 956
Hpy166II GTNNAC 3 cut(s) 93, 305, 456
Hpy188I TCNGA 9 cut(s) 34, 135, 213, 257, 423, 593, 729, 1021, 1269
Hpy188III TCNNGA 4 cut(s) 154, 283, 513, 1247
Hpy8I GTNNAC 3 cut(s) 93, 305, 456
Hpy99I CGWCG 3 cut(s) 710, 917, 1307
HpyAV CCTTC 5 cut(s) 181, 640, 826, 1097, 1264
HpyCH4III ACNGT 1 cut(s) 1174
HpyCH4IV ACGT 1 cut(s) 643
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 626, 852, 923, 1196, 1205
HpyF3I CTNAG 3 cut(s) 33, 777, 828
HpySE526I ACGT 1 cut(s) 643
Hsp92I GRCGYC 1 cut(s) 915
Hsp92II CATG 7 cut(s) 313, 550, 768, 880, 943, 1031, 1199
HspAI GCGC 1 cut(s) 46
KroI GCCGGC 1 cut(s) 995
KroNI GCCGGC 1 cut(s) 997
Ksp22I TGATCA 2 cut(s) 418, 745
LmnI GCTCC 2 cut(s) 869, 1246
Lsp1109I GCAGC 6 cut(s) 71, 302, 545, 710, 833, 903
LweI GCATC 1 cut(s) 1195
MaeI CTAG 3 cut(s) 224, 1100, 1137
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 1 cut(s) 962
MboII GAAGA 3 cut(s) 64, 421, 560
MflI RGATCY 2 cut(s) 208, 1139
MhlI GDGCHC 1 cut(s) 458
MlsI TGGCCA 1 cut(s) 944
MluCI AATT 8 cut(s) 80, 446, 519, 658, 669, 1074, 1114, 1257
MluNI TGGCCA 1 cut(s) 944
MlyI GAGTC 2 cut(s) 770, 1025
Mox20I TGGCCA 1 cut(s) 944
Mph1103I ATGCAT 1 cut(s) 1152
MroNI GCCGGC 1 cut(s) 995
MroXI GAANNNNTTC 2 cut(s) 1086, 1107
MscI TGGCCA 1 cut(s) 944
MseI TTAA 2 cut(s) 672, 1131
MslI CAYNNNNRTG 1 cut(s) 769
Msp20I TGGCCA 1 cut(s) 944
MspA1I CMGCKG 2 cut(s) 701, 894
MspI CCGG 3 cut(s) 365, 582, 996
MspR9I CCNGG 2 cut(s) 740, 788
Mva1269I GAATGC 2 cut(s) 292, 1107
MvaI CCWGG 2 cut(s) 740, 788
MwoI GCNNNNNNNGC 6 cut(s) 43, 626, 852, 923, 1196, 1205
NaeI GCCGGC 1 cut(s) 997
NgoMIV GCCGGC 1 cut(s) 995
NlaIII CATG 7 cut(s) 313, 550, 768, 880, 943, 1031, 1199
NmuCI GTSAC 1 cut(s) 962
NsiI ATGCAT 1 cut(s) 1152
PctI GAATGC 2 cut(s) 292, 1107
PdiI GCCGGC 1 cut(s) 997
PdmI GAANNNNTTC 2 cut(s) 1086, 1107
PfeI GAWTC 4 cut(s) 150, 818, 970, 1180
PflMI CCANNNNNTGG 2 cut(s) 181, 1201
PkrI GCNGC 7 cut(s) 61, 292, 535, 700, 703, 848, 893
PleI GAGTC 2 cut(s) 769, 1024
PpsI GAGTC 2 cut(s) 769, 1024
Psp6I CCWGG 2 cut(s) 738, 786
PspFI CCCAGC 1 cut(s) 528
PspGI CCWGG 2 cut(s) 738, 786
PspPI GGNCC 1 cut(s) 627
PsuI RGATCY 2 cut(s) 208, 1139
PvuII CAGCTG 1 cut(s) 894
RsaI GTAC 3 cut(s) 306, 718, 898
RsaNI GTAC 3 cut(s) 305, 717, 897
RseI CAYNNNNRTG 1 cut(s) 769
SaqAI TTAA 2 cut(s) 672, 1131
SatI GCNGC 7 cut(s) 60, 291, 534, 699, 702, 847, 892
Sau96I GGNCC 1 cut(s) 627
ScaI AGTACT 1 cut(s) 718
SchI GAGTC 2 cut(s) 770, 1025
ScrFI CCNGG 2 cut(s) 740, 788
SduI GDGCHC 1 cut(s) 458
SfaNI GCATC 1 cut(s) 1195
SfcI CTRYAG 1 cut(s) 802
SmiMI CAYNNNNRTG 1 cut(s) 769
Sse9I AATT 8 cut(s) 80, 446, 519, 658, 669, 1074, 1114, 1257
SsiI CCGC 4 cut(s) 333, 701, 1159, 1199
SspI AATATT 1 cut(s) 218
SspMI CTAG 3 cut(s) 224, 1100, 1137
StyD4I CCNGG 2 cut(s) 738, 786
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 1 cut(s) 1174
TaiI ACGT 1 cut(s) 646
TaqI TCGA 2 cut(s) 633, 954
TasI AATT 8 cut(s) 80, 446, 519, 658, 669, 1074, 1114, 1257
TatI WGTACW 2 cut(s) 716, 896
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 4 cut(s) 150, 818, 970, 1180
Tru1I TTAA 2 cut(s) 672, 1131
Tru9I TTAA 2 cut(s) 672, 1131
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 962
TseI GCWGC 6 cut(s) 59, 290, 533, 698, 846, 891
Tsp45I GTSAC 1 cut(s) 962
TspDTI ATGAA 4 cut(s) 374, 421, 865, 1083
TspGWI ACGGA 1 cut(s) 1223
TspRI CASTG 1 cut(s) 250
Van91I CCANNNNNTGG 2 cut(s) 181, 1201
VneI GTGCAC 1 cut(s) 454
XapI RAATTY 4 cut(s) 446, 519, 658, 1114
XmnI GAANNNNTTC 2 cut(s) 1086, 1107
XspI CTAG 3 cut(s) 224, 1100, 1137
ZrmI AGTACT 1 cut(s) 718
Zsp2I ATGCAT 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.